fps1 (SPAC6F12.13c)


Gene Standard Namefps1 Characterisation Statuspublished
Systematic IDSPAC6F12.13c Feature Typeprotein coding
Synonyms Name Description
Productgeranyltranstransferase Fps1 Product Size347aa, 39.52 kDa
Genomic Location Chromosome I, 1339942-1338365 (1578nt); CDS:1339700-1338452 (1249nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004161dimethylallyltranstransferase activity2
heterologous_systemIGISGD:S000003703PMID:17596513
GO:0004337geranyltranstransferase activity2
heterologous_systemISOSGD:S000003703PMID:17596513
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037747
GO:0005515protein bindingIPIspo9PMID:17596513831
GO:0004660protein farnesyltransferase activity4
contributes_toIMPPMID:17596513
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006995cellular response to nitrogen starvationIMPPMID:1936672886
GO:0006696ergosterol biosynthetic process44
heterologous_systemIGISGD:S000003703PMID:17596513
GO:0045337farnesyl diphosphate biosynthetic process4
heterologous_systemIGISGD:S000003703PMID:17596513
GO:0033384geranyl diphosphate biosynthetic processIEA UniPathway:UPA00259GO_REF:00000412
GO:0008299isoprenoid biosynthetic process16
heterologous_systemISOSGD:S000003703PMID:17596513
GO:0070317negative regulation of G0 to G1 transitionIMPPMID:1936672825
GO:0018342protein prenylationICGO:0004660GO_REF:00000017
GO:0072661protein targeting to plasma membraneICGO:0018342GO_REF:00000017
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722315
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000082decreased cell population growth at high temperatureCell growth assayfps1-439PECO:0000004, PECO:0000099PMID:19366728148
FYPO:0000709decreased cell population growth during nitrogen starvationCell growth assayfps1-439PECO:0000004, PECO:0000099PMID:1936672835
FYPO:0002061inviable vegetative cell populationMicroscopynot recorded (unrecorded)PMID:175965131338
Microscopyfps1ΔNullPMID:20473289
Microscopyfps1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002151inviable spore465
penetrance FYPO_EXT:0000001Microscopyfps1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
113399421339869
213397001339581
313393751338365

UTRs

Region Coordinates Reference
five_prime_UTR1339942..1339869PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00348 Pfam IPR000092 Polyprenyl synthetase 37 306 3
PS00723 Prosite Patterns IPR000092 Polyprenyl synthetase 92 106 3
PS00444 Prosite Patterns IPR000092 Polyprenyl synthetase 227 239 3
PTHR11525:SF0 HMMPANTHER 7 347 2
PTHR11525 HMMPANTHER 7 347 2
1.10.600.10 Gene3D IPR008949 Terpenoid synthase 4 347 5
SSF48576 SuperFamily IPR008949 Terpenoid synthase 5 346 5

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.88 Da
Charge -11.00
Isoelectric point 4.73
Molecular weight 39.52 kDa
Number of residues 347
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
269372during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
264317during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
262841during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
253275during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
48394.43during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
254592during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
18670.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
20during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complements S. cerevisiae ERG20 PMID:17596513
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
spo9farnesyl pyrophosphate synthetase Dosage RescuePMID:17596513
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
fps1geranyltranstransferase Fps1 Two-hybridPMID:17596513
spo9farnesyl pyrophosphate synthetase Affinity Capture-WesternPMID:17596513
Two-hybrid
cwf2RNA-binding protein Cwf2 Affinity Capture-MSPMID:21386897
External References
Database Identifier Description
NBRP SPAC6F12.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC6F12.13c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC6F12.13c BioGRID Interaction Datasets
Expression Viewer SPAC6F12.13c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC6F12.13c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC6F12.13c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC6F12.13c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC6F12.13c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC6F12.13c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC6F12.13c Cell Cycle Data
GEO SPAC6F12.13c GEO profiles
PInt SPAC6F12.13c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC6F12.13c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC6F12.13c Fission yeast phenotypic data & analysis
SPD / RIKEN44/44A12Orfeome Localization Data
IntEnz2.5.1.10Integrated relational Enzyme database
Rhea2.5.1.10Annotated reactions database
UniProtKB/SwissProtO14230Farnesyl pyrophosphate synthase
ModBaseO14230Database of comparative protein structure models
STRINGO14230Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593299geranyltranstransferase Fps1
RefSeq mRNANM_001018729972h- geranyltranstransferase Fps1 (fps1), mRNA
European Nucleotide ArchiveD89104ENA EMBL mapping
European Nucleotide ArchiveCAB11097.1ENA Protein Mapping
UniParcUPI000012ABBAUniProt Archive

Literature for fps1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014