fps1 (SPAC6F12.13c)


Gene Standard Namefps1 Characterisation Statuspublished
Systematic IDSPAC6F12.13c Feature Typeprotein coding
Synonyms Name Description
Productgeranyltranstransferase Fps1 Product Size347aa, 39.52 kDa
Genomic Location Chromosome I, 1339942-1338365 (1578nt); CDS:1339700-1338452 (1249nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
dimethylallyltranstransferase activity1
Annotation ExtensionEvidenceWith/FromReference
farnesyltranstransferase activity4
Annotation ExtensionEvidenceWith/FromReference
geranyltranstransferase activity2
Annotation ExtensionEvidenceWith/FromReference
metal ion binding750
Annotation ExtensionEvidenceWith/FromReference
protein binding863
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ergosterol biosynthetic process45
Annotation ExtensionEvidenceWith/FromReference
farnesyl diphosphate biosynthetic process4
Annotation ExtensionEvidenceWith/FromReference
geranyl diphosphate biosynthetic process2
Annotation ExtensionEvidenceWith/FromReference
geranylgeranyl diphosphate biosynthetic process2
Annotation ExtensionEvidenceWith/FromReference
isoprenoid biosynthetic process16
Annotation ExtensionEvidenceWith/FromReference
negative regulation of G0 to G1 transition23
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationfps1Δ1453
loss of viability upon nitrogen starvationfps1-43953

Cell Phenotype

Term NameGenotypesCount
inviable after spore germination, single or double cell divisionfps1Δ106
inviable sporefps1Δ476
normal farnesyltranstransferase activityfps1-R104Q (R104Q)1

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
viable vegetative cell populationspo9Δ, fps1+

Cell Phenotype

Term NameGenotypes
decreased farnesyltranstransferase activityspo9-R109Q (R109Q), fps1-R104Q (R104Q)
normal sporulationspo9Δ, fps1+
Target Of
OntologyRelationshipGeneProduct
GO substrate of sst2 human AMSH/STAMBP protein homolog, ubiquitin specific-protease
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
GO substrate of ubp9 ubiquitin C-terminal hydrolase Ubp9
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1339942..1339869, 1339700..1339581, 1339375..1338365
Intron1339868..1339701, 1339580..1339376
5' UTR1339942..1339869PMID:21511999
3' UTR1338451..1338365SPC10849
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00348 Pfam IPR000092 Polyprenyl synthetase 42 303 4
PS00444 Prosite Patterns IPR000092 Polyprenyl synthetase 227 239 3
PS00723 Prosite Patterns IPR000092 Polyprenyl synthetase 92 106 3
PTHR11525:SF0 HMMPANTHER 5 347 1
PTHR11525 HMMPANTHER 5 347 2
1.10.600.10 Gene3D IPR008949 Isoprenoid synthase domain 4 347 5
SSF48576 SuperFamily IPR008949 Isoprenoid synthase domain 5 346 5

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.88 Da
Charge -11.00
Codon Adaptation Index 0.56
Isoelectric point 4.73
Molecular weight 39.52 kDa
Number of residues 347
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0072690Northern assay evidencePMID:17596513
decreased during GO:0006995Northern assay evidencePMID:17596513

Quantitative Gene Expression

View graphical display of gene expression data for fps1 (SPAC6F12.13c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
269372during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
264317during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
262841during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
253275during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
254592during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
48394.43during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
18670.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
20during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complements S. cerevisiae ERG20 PMID:17596513
functionally complemented by S. cerevisiae ERG20 PMID:17596513
functionally complements S. cerevisiae BTS1 PMID:17596513
functionally complements E. coli ispA PMID:17596513
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC6F12.13c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withfps1geranyltranstransferase Fps1 Two-hybridPMID:17596513
binds activation domain construct withspo9farnesyl pyrophosphate synthetase Two-hybridPMID:17596513
affinity captured bycwf2RNA-binding protein Cwf2 Affinity Capture-MSPMID:21386897
binds DNA-binding domain construct withspo9farnesyl pyrophosphate synthetase Two-hybridPMID:17596513
affinity capturesspo9farnesyl pyrophosphate synthetase Affinity Capture-WesternPMID:17596513
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC6F12.13c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
overexpression rescuesspo9farnesyl pyrophosphate synthetase Dosage RescuePMID:17596513
External References
Database Identifier Description
NBRP SPAC6F12.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC6F12.13c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC6F12.13c BioGRID Interaction Datasets
Expression Viewer SPAC6F12.13c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC6F12.13c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC6F12.13c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC6F12.13c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC6F12.13c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC6F12.13c Transcriptome Viewer (Bähler Lab)
GEO SPAC6F12.13c GEO profiles
PInt SPAC6F12.13c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC6F12.13c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC6F12.13c Fission yeast phenotypic data & analysis
Cyclebase SPAC6F12.13c.1 Cell Cycle Data
IntEnz2.5.1.10Integrated relational Enzyme database
Rhea2.5.1.10Annotated reactions database
SPD / RIKEN44/44A12Orfeome Localization Data
UniProtKB/SwissProtO14230Farnesyl pyrophosphate synthase
ModBaseO14230Database of comparative protein structure models
STRINGO14230Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593299geranyltranstransferase Fps1
RefSeq mRNANM_001018729972h- geranyltranstransferase Fps1 (fps1), mRNA

Literature for fps1

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016