tpz1 (SPAC6F6.16c)

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Gene Standard Nametpz1 Characterisation Statuspublished
Systematic IDSPAC6F6.16c Feature Typeprotein coding
Synonymsmug169, SPAC6F6.18c Name DescriptionMeiotically Upregulated Gene
ProductTppl homolog Tpz1 Product Size508aa, 57.68 kDa
Genomic Location Chromosome I, 2765950-2762475 (3476nt); CDS:2765433-2763686 (1748nt)

Ensembl Gene Location
  1. SPAC6F6.13c
  2. SPAC6F6.19-antisense-1
  3. SPAC6F6.19
  4. ypt5
  5. tpz1
  6. rif1
  7. rif1-antisense-1
  8. ppk6
GO Molecular Function
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Term NameCount
protein binding900
protein binding involved in negative regulation of telomere maintenance via telomerase1
Annotation ExtensionEvidenceWith/FromReference
  • binds poz1, residue 426-450
  • binds pot1, residue 488-499
telomeric DNA binding14
GO Biological Process

GO Slim Terms

telomere organization


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GO Cellular Component
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FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable


Population Phenotype

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Term NameGenotypesCount
inviable vegetative cell populationtpz1Δ1468
sensitive to thiabendazoletpz1Δ334

Cell Phenotype

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Term NameGenotypesCount
abnormal mitotic cell cycletpz1Δ903
abolished protein-protein interaction164
affecting tpz1 and poz1tpz1-I501R (I501R)
affecting tpz1 and poz1tpz1-I501A/R505E (I501A, R505E)
affecting tpz1 and poz1tpz1-W498R,I501R (W498R, I501R)
affecting tpz1 and pot1tpz1-I200R (I200R)
affecting tpz1 and ccq1tpz1-L449R (L449R)
affecting tpz1 and ccq1tpz1-Y439R,L445R (Y439R, L445R)
affecting tpz1 and ccq1tpz1-L449A (L449A)
circularized chromosometpz1-[1-485]-L449R (Deletion of amino acids 485-508 (poz1-interaction domain) & point mutation L449R)8
tpz1-L449R (L449R)
tpz1-Y439R,L445R (Y439R, L445R)
tpz1-L449A (L449A)
tpz1-L449R,W498R,I501R (L449R, W498R, I501R)
decreased chromatin silencing at centromere outer repeattpz1Δ51
decreased chromatin silencing at subtelomeretpz1-[1-485] (Deletion of amino acids 485-508 (poz1-interaction domain))12
tpz1-Y439R,L445R (Y439R, L445R)
tpz1-W498R,I501R (W498R, I501R)
tpz1-L449R (L449R)
decreased protein localization to telomere11
affects localization of poz1tpz1-[1-485]-L449R (Deletion of amino acids 485-508 (poz1-interaction domain) & point mutation L449R)
affects localization of poz1tpz1-W498R,I501R (W498R, I501R)
affects localization of trt1tpz1-Y439R,L445R (Y439R, L445R)
affects localization of trt1tpz1-R81E (R81E)
affects localization of trt1tpz1-L449R (L449R)
affects localization of trt1tpz1-I77R (I77R)
decreased protein-protein interaction109
affecting tpz1 and trt1tpz1-R81E (R81E)
affecting tpz1 and trt1tpz1-I77R (I77R)
elongated telomerestpz1-L449A/I501R (L449A, I501R)176
tpz1-W498R,I501R (W498R, I501R)
tpz1-I501A/R505E (I501A, R505E)
tpz1-[1-485] (Deletion of amino acids 485-508 (poz1-interaction domain))
tpz1-I200R (I200R)
tpz1-I501R (I501R)
increased mitotic DNA damage checkpoint activationtpz1-L449A (L449A)4
increased protein localization to telomere during vegetative growth5
affects localization of trt1tpz1-[1-485]-L449R (Deletion of amino acids 485-508 (poz1-interaction domain) & point mutation L449R)
affects localization of trt1tpz1-W498R,I501R (W498R, I501R)
increased protein phosphorylation during vegetative growth66
affecting chk1tpz1-L449R (L449R)
affecting chk1tpz1-Y439R,L445R (Y439R, L445R)
inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cellstpz1Δ88
normal protein localization to telomere during vegetative growth14
affecting poz1tpz1-I501A/R505E (I501A, R505E)
affecting pot1tpz1-I501R (I501R)
affecting pot1tpz1-I200R (I200R)
affecting tpz1tpz1-I77R (I77R)
affecting tpz1tpz1-E74R (E74R)
affecting tpz1tpz1-R81E (R81E)
affecting tpz1tpz1-K75E (K75E)
affecting tpz1tpz1-R76E (R76E)
affecting trt1tpz1-K75E (K75E)
affecting trt1tpz1-E74R (E74R)
affecting trt1tpz1-L449A (L449A)
affecting trt1tpz1-R76E (R76E)
affecting ccq1tpz1-I77R (I77R)
affecting ccq1tpz1-K75E (K75E)
affecting ccq1tpz1-R76E (R76E)
affecting ccq1tpz1-R81E (R81E)
affecting ccq1tpz1-L449A (L449A)
affecting ccq1tpz1-E74R (E74R)
normal protein-protein interaction107
affecting tpz1 and trt1tpz1-R76E (R76E)
affecting tpz1 and trt1tpz1-E74R (E74R)
affecting tpz1 and trt1tpz1-K75E (K75E)
affecting tpz1 and trt1tpz1-K75A (K75A)
normal telomere lengthtpz1-E116R (E116R)35
tpz1-K124E (K124E)
tpz1-E74R (E74R)
tpz1-E108R (E108R)
progressively shortening telomerestpz1-L449R (L449R)7
tpz1-R81E (R81E)
tpz1-Y439R,L445R (Y439R, L445R)
tpz1-I77R (I77R)
tpz1-L449A (L449A)
shortened telomerestpz1-F112A (F112A)44
stable shortened telomerestpz1-L449R (L449R)5
tpz1-K75E (K75E)
tpz1-T73A (T73A)
tpz1-Y439R,L445R (Y439R, L445R)
tpz1-R76E (R76E)
tpz1-L449A (L449A)
viable elongated vegetative celltpz1-L449R (L449R)329
tpz1-Y439R,L445R (Y439R, L445R)
viable elongated vegetative cell, with progressive elongationtpz1-L449A (L449A)4

FYPO Multi-allele Phenotypes

Population Phenotype

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Term NameGenotypes
sensitive to thiabendazoletpz1Δ, dcr1Δ

Cell Phenotype

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Term NameGenotypes
circularized chromosomepot1-I453R (I453R), tpz1-I200R (I200R)
poz1Δ, tpz1-R81E (R81E)
pot1-F520A (F520A), tpz1-I200R (I200R)
decreased chromatin silencing at centromere outer repeattpz1Δ, dcr1Δ
elongated telomerestpz1-I501R (I501R), poz1-R196E (R196E)
progressively shortening telomerespoz1Δ, tpz1-R81E (R81E)
tpz1-R81E (R81E), trt1Δ
stable shortened telomeresccq1-F157A,K174E (F157A, K174E), tpz1-L449A (L449A)
Target Of
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OntologyRelationshipGeneProduct
FYPO affected by mutation in ccq1 telomere maintenance protein Ccq1
FYPO affected by mutation in pot1 telomere end-binding protein Pot1
FYPO affected by mutation in poz1 Pot1 associated protein Poz1
FYPO affected by mutation in tpz1 Tppl homolog Tpz1
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2765950..2765804, 2765706..2765320, 2765263..2765166, 2765122..2764872, 2764825..2764791, 2764752..2764611, 2764572..2762475
Intron2765803..2765707, 2765319..2765264, 2765165..2765123, 2764871..2764826, 2764790..2764753, 2764610..2764573
mRNA2765950..2762475
5' UTR2765950..2765804, 2765706..2765434PMID:21511999
CDS2765433..2765320, 2765263..2765166, 2765122..2764872, 2764825..2764791, 2764752..2764611, 2764572..2763686
3' UTR2763685..2762475PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PTHR14487 HMMPANTHER IPR028631 Adrenocortical dysplasia protein 373 508 1
PTHR14487 HMMPANTHER IPR028631 Adrenocortical dysplasia protein 1 356 1

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001718OB foldPMID:18535244Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001718

Protein Properties

Ave. residue weight 113.55 Da
Charge -19.00
Codon Adaptation Index 0.41
Isoelectric point 4.73
Molecular weight 57.68 kDa
Number of residues 508
Sequence
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Gene Expression
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Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0051321experimental evidencePMID:16303567
increased during GO:0051321experimental evidencePMID:16303567
increased during GO:0051321experimental evidencePMID:16303567
increased during GO:0051321experimental evidencePMID:16303567
increased during GO:0051321experimental evidencePMID:16303567

Quantitative Gene Expression

View graphical display of gene expression data for tpz1 (SPAC6F6.16c)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.1during GO:0072690PECO:0000126,
PECO:0000005		population wideexperimental evidencePMID:23101633
1.1during GO:0072690PECO:0000126,
PECO:0000005		population wideexperimental evidencePMID:23101633
1.1during GO:0072690PECO:0000126,
PECO:0000005		population wideexperimental evidencePMID:23101633
1.1during GO:0072690PECO:0000126,
PECO:0000005		population wideexperimental evidencePMID:23101633
1.1during GO:0072690PECO:0000126,
PECO:0000005		population wideexperimental evidencePMID:23101633
0.42during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005		population wideexperimental evidencePMID:23101633
0.42during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005		population wideexperimental evidencePMID:23101633
0.42during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005		population wideexperimental evidencePMID:23101633
0.42during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005		population wideexperimental evidencePMID:23101633
0.42during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005		population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
Dyskeratosis congenita1
Warning
DescriptionQualifierReferenceCount
gene structure updated435
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3087
conserved in fungi4608
conserved in eukaryotes4516
conserved in metazoa3498
conserved in vertebrates3473
conserved in eukaryotes only2525
Orthologs
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Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC6F6.16c into the Query Builder
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View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesccq1telomere maintenance protein Ccq1 Affinity Capture-WesternPMID:24711392
affinity capturestrt1telomerase reverse transcriptase 1 protein Trt1 Affinity Capture-WesternPMID:25131669
affinity capturespoz1Pot1 associated protein Poz1 Affinity Capture-WesternPMID:24711392
affinity capturespot1telomere end-binding protein Pot1 Affinity Capture-WesternPMID:26365187
binds DNA-binding domain construct withpoz1Pot1 associated protein Poz1 Two-hybridPMID:26771498
binds DNA-binding domain construct withstn1telomere cap complex subunit Stn1 Two-hybridPMID:24925530
forms complex withccq1telomere maintenance protein Ccq1 Reconstituted ComplexPMID:24013504
forms complex withpoz1Pot1 associated protein Poz1 Reconstituted ComplexPMID:24013504
forms complex withpot1telomere end-binding protein Pot1 Reconstituted ComplexPMID:24013504
binds activation domain construct withccq1telomere maintenance protein Ccq1 Two-hybridPMID:25131669
binds activation domain construct withpoz1Pot1 associated protein Poz1 Two-hybridPMID:25330395
binds activation domain construct withpot1telomere end-binding protein Pot1 Two-hybridPMID:25330395
affinity captured bypoz1Pot1 associated protein Poz1 Affinity Capture-WesternPMID:24711392
affinity captured bypmt3ubiquitin-like protein modifier SUMO Affinity Capture-WesternPMID:24925530
affinity captured byccq1telomere maintenance protein Ccq1 Affinity Capture-WesternPMID:26365187
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC6F6.16c into the Query Builder
View these interactions in esyN

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Gene Product Evidence Reference
synthetic growth defect withpoz1Pot1 associated protein Poz1 Synthetic Growth DefectPMID:25330395
synthetic growth defect withstn1telomere cap complex subunit Stn1 Synthetic Growth DefectPMID:24925530
rescuesdcr1dicer Phenotypic SuppressionPMID:24240238
enhances phenotype ofccq1telomere maintenance protein Ccq1 Phenotypic EnhancementPMID:24013504
negative genetic interaction withSPAC17H9.08mitochondrial coenzyme A transmembrane transporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction withulp2SUMO deconjugating cysteine peptidase Ulp2 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withSPAC227.17cconserved eukaryotic protein, human c22orf39 ortholog Positive GeneticPMID:22681890
phenotype enhanced bypot1telomere end-binding protein Pot1 Phenotypic EnhancementPMID:26365187
External References
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Database Identifier Description
NBRP SPAC6F6.16c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC6F6.16c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC6F6.16c BioGRID Interaction Datasets
Expression Viewer SPAC6F6.16c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC6F6.16c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC6F6.16c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC6F6.16c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC6F6.16c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC6F6.16c Transcriptome Viewer (Bähler Lab)
GEO SPAC6F6.16c GEO profiles
PInt SPAC6F6.16c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC6F6.16c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC6F6.16c Fission yeast phenotypic data & analysis
Cyclebase SPAC6F6.16c.1 Cell Cycle Data
SPD / RIKEN11/11A11Orfeome Localization Data
UniProtKB/SwissProtO14246Protection of telomeres protein tpz1
ModBaseO14246Database of comparative protein structure models
STRINGO14246Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593908telomere-protecting protein
RefSeq mRNANM_001019338972h- telomere-protecting protein (tpz1), mRNA
European Nucleotide ArchiveAB433170ENA EMBL mapping
European Nucleotide ArchiveCAB11738.2ENA Protein Mapping
UniParcUPI0001765F0AUniProt Archive

Literature for tpz1
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Release Version: PomBase:30_62 - 30 Jan 2017