cfr1 (SPAC6G9.12)


Gene Standard Namecfr1 Characterisation Statuspublished
Systematic IDSPAC6G9.12 Feature Typeprotein coding
Synonyms Name Description
Productexomer complex BRCT domain subunit Cfr1 Product Size620aa, 67.23 kDa
Genomic Location Chromosome I, 3270191-3272367 (2177nt); CDS:3270235-3272097 (1863nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
Golgi to endosome transport11
Annotation ExtensionEvidenceWith/FromReference
Golgi to plasma membrane transport12
Annotation ExtensionEvidenceWith/FromReference
Golgi to vacuole transport19
Annotation ExtensionEvidenceWith/FromReference
NOT chitin biosynthetic process
Annotation ExtensionEvidenceWith/FromReference
NOT glucan biosynthetic process
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2298
Annotation ExtensionEvidenceWith/FromReference
early endosome2
Annotation ExtensionEvidenceWith/FromReference
exomer complex2
Annotation ExtensionEvidenceWith/FromReference
Golgi apparatus358
Annotation ExtensionEvidenceWith/FromReference
trans-Golgi network8
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth at high temperaturecfr1Δ186
decreased cell population growth on glucose carbon sourcecfr1Δ393
normal cell population growth at low temperaturecfr1Δ33
normal growth on glucose carbon sourcecfr1Δ318
resistance to lithiumcfr1Δ4
sensitive to caspofungincfr1Δ7
sensitive to dithiothreitolcfr1Δ6
sensitive to potassium chloridecfr1Δ30
sensitive to salt stresscfr1Δ102
sensitive to tunicamycincfr1Δ8
viable vegetative cell populationcfr1Δ3862

Cell Phenotype

Term NameGenotypesCount
abnormal cell separation after cytokinesis resulting in septated cellcfr1Δ23
abnormal protein localization during vegetative growth457
affects localization of bch1cfr1Δ
decreased cell wall beta-glucan levelcfr1Δ13
excess Golgi cisternae presentcfr1Δ2
increased cell wall alpha-glucan levelcfr1Δ10
increased protein secretion during vegetative growth15
affects localization of cpy1cfr1Δ
normal endocytosiscfr1Δ10
normal protein localization during vegetative growth293
affecting pma1cfr1Δ
affecting gas1cfr1Δ
affecting bgs3cfr1Δ
affecting rho1cfr1Δ
affecting cfr1ΔCT (C terminal deletion)
affecting chr4cfr1Δ
affecting chs2cfr1Δ
affecting bgs1cfr1Δ
affecting gas2cfr1Δ
affecting clc1cfr1Δ
affecting apm1cfr1Δ
affecting ags1cfr1Δ
affecting bgs4cfr1Δ
normal protein localization to cell-cell contact point during conjugation2
affecting cfr1cfr1Δ
normal vacuolar morphology during vegetative growthcfr1Δ3
viable vegetative cell with normal cell morphologycfr1Δ3103

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
abolished cell population growth at high temperaturecfr1Δ, cps1-191
abolished cell population growth at low temperaturecfr1Δ, gga21Δ, gga22Δ
decreased cell population growth at high temperaturegga22Δ, cfr1Δ
cfr1Δ, gga21Δ, gga22Δ
decreased cell population growth at low temperaturecfr1Δ, gga22Δ
decreased cell population growth on glucose carbon sourceend4Δ, cfr1Δ
apm1Δ, cfr1Δ
cfr1Δ, gga21Δ, gga22Δ
normal cell population growth at high temperaturegga21Δ, cfr1Δ
cfr1Δ, apl3Δ
normal cell population growth at low temperaturegga21Δ, cfr1Δ
normal growth on glucose carbon sourceapm3Δ, cfr1Δ
cfr1Δ, gga21Δ
sensitive to caspofunginapm1Δ, cfr1Δ
cfr1Δ, cps1-191

Cell Phenotype

Term NameGenotypes
abnormal Golgi morphologycfr1Δ, apm1Δ
decreased 1,3-beta-D-glucan synthase activitycfr1Δ, apm1Δ
decreased protein localization to plasma membrane of cell tip during vegetative growth
affects localization of bgs3cfr1Δ, apm1Δ
affects localization of bgs1cfr1Δ, apm1Δ
affects localization of ags1cfr1Δ, apm1Δ
affects localization of bgs4cfr1Δ, apm1Δ
large vacuoles during vegetative growthgga21Δ, cfr1Δ, gga22Δ
normal protein level during vegetative growth
affecting bgs3cfr1Δ, apm1Δ
affecting bgs1cfr1Δ, apm1Δ
affecting ags1cfr1Δ, apm1Δ
affecting bgs4cfr1Δ, apm1Δ
small vacuoles during vegetative growthcfr1Δ, apm1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO localization affected by mutation in bch1 exocyst complex ChAPs family (Chs5p-Arf1p-binding) protein Bch1
GO substrate of sst2 human AMSH/STAMBP protein homolog, ubiquitin specific-protease
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
GO substrate of ubp9 ubiquitin C-terminal hydrolase Ubp9
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3270191..3272367
mRNA3270191..3272367
5' UTR3270191..3270234PMID:21511999
CDS3270235..3272097
3' UTR3272098..3272367PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF12738 Pfam IPR001357 BRCT domain 176 237 4
PF16893 Pfam IPR031669 Fibronectin type III domain, fungi 79 166 1
PF16892 Pfam IPR031673 Chitin biosynthesis protein Chs5, N-terminal 6 53 1
SM00292 SMART IPR001357 BRCT domain 169 246 10
SM00060 SMART IPR003961 Fibronectin type III 79 157 1
PS50853 Prosite Profiles IPR003961 Fibronectin type III 79 169 1
PS50172 Prosite Profiles IPR001357 BRCT domain 172 256 12
PTHR13561 HMMPANTHER 13 615 3
PTHR13561:SF28 HMMPANTHER 13 615 1
3.40.50.10190 Gene3D IPR001357 BRCT domain 172 258 13
2.60.40.10 Gene3D IPR013783 Immunoglobulin-like fold 81 167 3
SSF52113 SuperFamily IPR001357 BRCT domain 171 256 12
SSF49265 SuperFamily IPR003961 Fibronectin type III 73 168 1
Coil ncoils Predicted coiled-coil protein (DUF2205) 434 457 1049

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000443BRCT domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000443

Protein Properties

Ave. residue weight 108.44 Da
Charge -64.00
Codon Adaptation Index 0.44
Isoelectric point 4.00
Molecular weight 67.23 kDa
Number of residues 620
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2293
present during mitotic M phaseS490
present during mitotic M phaseS611
present during mitotic M phaseS610
present during mitotic M phaseS532
present during mitotic M phaseS403
present during mitotic M phaseS510
present during mitotic M phaseS391
S270, S351, S360, S391, S466, S470, S471, S490, S532, S590, S591
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S490 PMID:21712547
present during mitotic M phase experimental evidence S611 PMID:21712547
present during mitotic M phase experimental evidence S610 PMID:21712547
present during mitotic M phase experimental evidence S532 PMID:21712547
present during mitotic M phase experimental evidence S403 PMID:21712547
present during mitotic M phase experimental evidence S510 PMID:21712547
present during mitotic M phase experimental evidence S391 PMID:21712547
IDA S270 PMID:25720772
experimental evidence S532 PMID:24763107
experimental evidence S590 PMID:24763107
experimental evidence S391 PMID:24763107
IDA S351 PMID:25720772
IDA S466 PMID:25720772
IDA S360 PMID:25720772
IDA S470 PMID:25720772
experimental evidence S591 PMID:24763107
IDA S471 PMID:25720772
experimental evidence S490 PMID:24763107
O-phospho-L-threonine 1087
present during mitotic M phaseT528
present during mitotic M phaseT396
T359, T467, T569
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T528 PMID:21712547
present during mitotic M phase experimental evidence T396 PMID:21712547
IDA T359 PMID:25720772
IDA T467 PMID:25720772
experimental evidence T569 PMID:24763107
phosphorylated residue 2515
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysineK241 514
Annotation ExtensionEvidenceResidueReference
IDA PMID:26412298
mass spectrometry evidence K241 PMID:26412298
O-phosphorylated residueS267,S270, S466,S470 2461
Annotation ExtensionEvidenceResidueReference
IDA S267,S270 PMID:25720772
IDA S466,S470 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for cfr1 (SPAC6G9.12)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
11536during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
12540during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
11884during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
10977during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14814.87during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
11916during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14046.74during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.6during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.91during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
does not functionally complement S. cerevisiae CHS5 PMID:21113731
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3087
conserved in fungi only552
conserved in fungi4608
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC6G9.12 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withbch1exocyst complex ChAPs family (Chs5p-Arf1p-binding) protein Bch1 Two-hybridPMID:26771498
binds activation domain construct withSPBC428.15Obg-like ATPase (predicted) Two-hybridPMID:26771498
affinity captured byapm4AP-2 adaptor complex mu subunit Apm4 (predicted) Affinity Capture-WesternPMID:27974503
affinity captured bygga22Golgi localized Arf binding gamma-adaptin ortholog Gga22 Affinity Capture-WesternPMID:27974503
affinity captured byapm1AP-1 adaptor complex mu subunit Apm1 Affinity Capture-WesternPMID:27974503
affinity captured bybch1exocyst complex ChAPs family (Chs5p-Arf1p-binding) protein Bch1 Affinity Capture-WesternPMID:27974503
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC6G9.12 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withsfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withwhi5cell cycle transcriptional repressor Whi5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
enhances phenotype offus1formin Fus1 Phenotypic EnhancementPMID:16598689
phenotype enhanced bygga22Golgi localized Arf binding gamma-adaptin ortholog Gga22 Phenotypic EnhancementPMID:27974503
phenotype enhanced bygga21Golgi localized Arf binding gamma-adaptin ortholog Gga21 Phenotypic EnhancementPMID:27974503
phenotype enhanced bysst4sorting receptor for ubiquitinated membrane proteins, ESCRT 0 complex subunit Sst4 Phenotypic EnhancementPMID:27974503
phenotype enhanced byapm1AP-1 adaptor complex mu subunit Apm1 Phenotypic EnhancementPMID:27974503
synthetic growth defect withgga22Golgi localized Arf binding gamma-adaptin ortholog Gga22 Synthetic Growth DefectPMID:27974503
synthetic growth defect withbgs1primary septum and spore wall linear 1,3-beta-glucan synthase catalytic subunit Bgs1 Synthetic Growth DefectPMID:27974503
synthetic growth defect withapm1AP-1 adaptor complex mu subunit Apm1 Synthetic Growth DefectPMID:27974503
synthetic growth defect withclc1clathrin light chain Clc1 Synthetic Growth DefectPMID:27974503
synthetic growth defect withend4Huntingtin-interacting protein homolog Synthetic Growth DefectPMID:27974503
External References
Database Identifier Description
NBRP SPAC6G9.12 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC6G9.12 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC6G9.12 BioGRID Interaction Datasets
Expression Viewer SPAC6G9.12 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC6G9.12 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC6G9.12 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC6G9.12 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC6G9.12 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC6G9.12 Transcriptome Viewer (Bähler Lab)
GEO SPAC6G9.12 GEO profiles
PInt SPAC6G9.12 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC6G9.12 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC6G9.12 Fission yeast phenotypic data & analysis
Cyclebase SPAC6G9.12.1 Cell Cycle Data
SPD / RIKEN26/26C05Orfeome Localization Data
UniProtKB/SwissProtQ92357Cell fusion protein cfr1
ModBaseQ92357Database of comparative protein structure models
STRINGQ92357Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594121Chs five related protein Cfr1
RefSeq mRNANM_001019545972h- Chs five related protein Cfr1 (cfr1), mRNA
European Nucleotide ArchiveCAB03614.1ENA Protein Mapping
UniParcUPI0000127924UniProt Archive

Literature for cfr1

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017