cfr1 (SPAC6G9.12)


Gene Standard Namecfr1 Characterisation Statuspublished
Systematic IDSPAC6G9.12 Feature Typeprotein coding
Synonyms Name Description
ProductChs five related protein Cfr1 Product Size620aa, 67.23 kDa
Genomic Location Chromosome I, 3270191-3272367 (2177nt); CDS:3270235-3272097 (1863nt)

Ensembl Gene Location
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006893Golgi to plasma membrane transportISOSGD:S000004322GO_REF:000002410
GO:0032219cell wall macromolecule catabolic process involved in cytogamyIMPPMID:165986891
different_pathwayIGIfus1PMID:16598689
GO:0032220plasma membrane fusion involved in cytogamyIMPPMID:165986896
NOT GO:0006031chitin biosynthetic processIMPPMID:16598689
NOT GO:0009250glucan biosynthetic processIMPPMID:16598689
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005794Golgi apparatusIDAPMID:21113731355
IDAPMID:16598689
GO:0005829cytosolIDAPMID:168233722319
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopycfr1ΔNullPMID:204732893751
Microscopycfr1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000644normal protein localization during vegetative growth119
affecting cfr1MicroscopyΔCT (C terminal deletion)Not specifiedPMID:21113731
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopycfr1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in cfr1 Chs five related protein Cfr1 PMID:21113731
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
132701913272367
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00533 Pfam IPR001357 BRCT domain 171 242 8
SM00060 SMART IPR003961 Fibronectin, type III 79 157 1
SM00292 SMART IPR001357 BRCT domain 169 246 10
PS50172 Prosite Profiles IPR001357 BRCT domain 172 256 13
PS50853 Prosite Profiles IPR003961 Fibronectin, type III 80 166 1
PTHR13561 HMMPANTHER 6 566 3
2.60.40.10 Gene3D IPR013783 Immunoglobulin-like fold 81 167 3
3.40.50.10190 Gene3D IPR001357 BRCT domain 172 258 13
SSF52113 SuperFamily IPR001357 BRCT domain 171 256 12
SSF49265 SuperFamily IPR003961 Fibronectin, type III 73 168 1
Coil ncoils Rabaptin coiled-coil domain 434 458 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000443BRCT domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000443

Protein Properties

Ave. residue weight 108.44 Da
Charge -64.00
Isoelectric point 4.00
Molecular weight 67.23 kDa
Number of residues 620
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS610PMID:217125471670
present during mitotic M phase
present during mitotic M phaseS611PMID:21712547
present during mitotic M phaseS490PMID:21712547
S490PMID:24763107
present during mitotic M phaseS403PMID:21712547
present during mitotic M phaseS510PMID:21712547
S591PMID:24763107
S532PMID:24763107
S391PMID:24763107
present during mitotic M phaseS391PMID:21712547
present during mitotic M phaseS532PMID:21712547
S590PMID:24763107
MOD:00047O-phospho-L-threonineT396PMID:21712547690
present during mitotic M phase
T569PMID:24763107
present during mitotic M phaseT528PMID:21712547
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
11536during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
12540during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
11884during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
10977during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
14814.87during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
11916during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
14046.74during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.6during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.91during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
does not functionally complement S. cerevisiae CHS5 PMID:21113731
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only620
conserved in fungi4599
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
fus1formin Fus1 Phenotypic EnhancementPMID:16598689
rxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
sfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
whi5cell cycle transcriptional repressor Whi5 (predicted) Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPAC6G9.12 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC6G9.12 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC6G9.12 BioGRID Interaction Datasets
Expression Viewer SPAC6G9.12 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC6G9.12 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC6G9.12 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC6G9.12 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC6G9.12 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC6G9.12 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC6G9.12 Cell Cycle Data
GEO SPAC6G9.12 GEO profiles
PInt SPAC6G9.12 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC6G9.12 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN26/26C05Orfeome Localization Data
UniProtKB/SwissProtQ92357Cell fusion protein cfr1
ModBaseQ92357Database of comparative protein structure models
STRINGQ92357Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594121Chs five related protein Cfr1
RefSeq mRNANM_001019545972h- Chs five related protein Cfr1 (cfr1), mRNA
European Nucleotide ArchiveCAB03614.1ENA Protein Mapping
UniParcUPI0000127924UniProt Archive

Literature for cfr1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014