cfr1 (SPAC6G9.12)


Gene Standard Namecfr1 Characterisation Statuspublished
Systematic IDSPAC6G9.12 Feature Typeprotein coding
Synonyms Name Description
ProductChs five related protein Cfr1 Product Size620aa, 67.23 kDa
Genomic Location Chromosome I, 3270191-3272367 (2177nt); CDS:3270235-3272097 (1863nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
cell wall macromolecule catabolic process involved in cytogamy1
Annotation ExtensionEvidenceWith/FromReference
Golgi to plasma membrane transport10
Annotation ExtensionEvidenceWith/FromReference
plasma membrane fusion involved in cytogamy6
Annotation ExtensionEvidenceWith/FromReference
NOT chitin biosynthetic process
Annotation ExtensionEvidenceWith/FromReference
NOT glucan biosynthetic process
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
Golgi apparatus357
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationcfr1Δ3837

Cell Phenotype

Term NameGenotypesCount
normal protein localization during vegetative growth250
affecting cfr1ΔCT (C terminal deletion)
normal protein localization to cell-cell contact point during conjugation2
affecting cfr1cfr1Δ
viable vegetative cell with normal cell morphologycfr1Δ3100
Target Of
OntologyRelationshipGeneProduct
GO substrate of sst2 human AMSH/STAMBP protein homolog, ubiquitin specific-protease
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
GO substrate of ubp9 ubiquitin C-terminal hydrolase Ubp9
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3270191..3272367
5' UTR3270191..3270234PMID:21511999
3' UTR3272098..3272367PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF12738 Pfam IPR001357 BRCT domain 176 237 4
PF16892 Pfam IPR031673 Chitin biosynthesis protein Chs5, N-terminal 6 53 1
PF16893 Pfam IPR031669 Fibronectin type III domain, fungi 79 166 1
SM00060 SMART IPR003961 Fibronectin type III 79 157 1
SM00292 SMART IPR001357 BRCT domain 169 246 10
PS50853 Prosite Profiles IPR003961 Fibronectin type III 79 169 1
PS50172 Prosite Profiles IPR001357 BRCT domain 172 256 13
PTHR13561 HMMPANTHER 13 615 3
PTHR13561:SF28 HMMPANTHER 13 615 1
2.60.40.10 Gene3D IPR013783 Immunoglobulin-like fold 81 167 3
3.40.50.10190 Gene3D IPR001357 BRCT domain 172 258 13
SSF49265 SuperFamily IPR003961 Fibronectin type III 73 168 1
SSF52113 SuperFamily IPR001357 BRCT domain 171 256 12
Coil ncoils Predicted coiled-coil protein (DUF2205) 434 457 1057

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000443BRCT domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000443

Protein Properties

Ave. residue weight 108.44 Da
Charge -64.00
Codon Adaptation Index 0.44
Isoelectric point 4.00
Molecular weight 67.23 kDa
Number of residues 620
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS611
present during mitotic M phaseS490
present during mitotic M phaseS610
present during mitotic M phaseS391
present during mitotic M phaseS532
present during mitotic M phaseS510
present during mitotic M phaseS403
S391, S490, S532, S590, S591
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S611 PMID:21712547
present during mitotic M phase experimental evidence S490 PMID:21712547
present during mitotic M phase experimental evidence S610 PMID:21712547
present during mitotic M phase experimental evidence S391 PMID:21712547
present during mitotic M phase experimental evidence S532 PMID:21712547
present during mitotic M phase experimental evidence S510 PMID:21712547
present during mitotic M phase experimental evidence S403 PMID:21712547
experimental evidence S590 PMID:24763107
experimental evidence S490 PMID:24763107
experimental evidence S391 PMID:24763107
experimental evidence S532 PMID:24763107
experimental evidence S591 PMID:24763107
O-phospho-L-threonine 700
present during mitotic M phaseT396
present during mitotic M phaseT528
T569
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T396 PMID:21712547
present during mitotic M phase experimental evidence T528 PMID:21712547
experimental evidence T569 PMID:24763107
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysineK241 512
Annotation ExtensionEvidenceResidueReference
IDA PMID:26412298
mass spectrometry evidence K241 PMID:26412298
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for cfr1 (SPAC6G9.12)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
11536during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
12540during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
11884during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
10977during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
11916during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14814.87during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
14046.74during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.6during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.91during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
does not functionally complement S. cerevisiae CHS5 PMID:21113731
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only618
conserved in fungi4605
conserved in eukaryotes4515
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC6G9.12 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withcfp1ChAPs family protein Cfp1 Two-hybridPMID:26771498
binds activation domain construct withSPBC428.15Obg-like ATPase (predicted) Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC6G9.12 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withsfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withwhi5cell cycle transcriptional repressor Whi5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
enhances phenotype offus1formin Fus1 Phenotypic EnhancementPMID:16598689
External References
Database Identifier Description
NBRP SPAC6G9.12 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC6G9.12 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC6G9.12 BioGRID Interaction Datasets
Expression Viewer SPAC6G9.12 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC6G9.12 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC6G9.12 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC6G9.12 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC6G9.12 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC6G9.12 Transcriptome Viewer (Bähler Lab)
GEO SPAC6G9.12 GEO profiles
PInt SPAC6G9.12 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC6G9.12 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC6G9.12 Fission yeast phenotypic data & analysis
Cyclebase SPAC6G9.12.1 Cell Cycle Data
SPD / RIKEN26/26C05Orfeome Localization Data
UniProtKB/SwissProtQ92357Cell fusion protein cfr1
ModBaseQ92357Database of comparative protein structure models
STRINGQ92357Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594121Chs five related protein Cfr1
RefSeq mRNANM_001019545972h- Chs five related protein Cfr1 (cfr1), mRNA

Literature for cfr1

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016