tim14 (SPAC824.06)


Gene Standard Nametim14 Characterisation Statusbiological_role_inferred
Systematic IDSPAC824.06 Feature Typeprotein coding
Synonyms Name Description
ProductTIM23 translocase complex subunit Tim14 (predicted) Product Size140aa, 15.27 kDa
Genomic Location Chromosome I, 1694906-1696484 (1579nt); CDS:1695102-1695624 (523nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0001671ATPase activator activityISOSGD:S000003998GO_REF:00000243
GO:0030544Hsp70 protein bindingISMPFAM:PF00226GO_REF:000000126
GO:0015450P-P-bond-hydrolysis-driven protein transmembrane transporter activity11
contributes_toISSUniProtKB:Q07914GO_REF:0000001
GO:0051082unfolded protein bindingISOSGD:S000003998GO_REF:000002442
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032781positive regulation of ATPase activityISOSGD:S000003998GO_REF:00000243
GO:0006457protein foldingNASGO_REF:0000051111
GO:0030150protein import into mitochondrial matrixISOSGD:S000003998GO_REF:000002418
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371017
GO:0005739mitochondrionIDAPMID:16823372758
GO:0001405presequence translocase-associated import motorISOSGD:S000003998GO_REF:00000246
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopytim14ΔNullPECO:0000005, PECO:0000137PMID:236978061331

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002111inviable tapered vegetative cell134
penetrance FYPO_EXT:0000001Microscopytim14ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
116949061695104
216951521695199
316952531696484

UTRs

Region Coordinates Reference
five_prime_UTR1694906..1695101PMID:21511999
three_prime_UTR1695625..1696484PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00226 Pfam IPR001623 DnaJ domain 54 108 24
SM00271 SMART IPR001623 DnaJ domain 50 105 23
PS50076 Prosite Profiles IPR001623 DnaJ domain 51 113 24
PTHR12763:SF28 HMMPANTHER IPR027134 Mitochondrial import inner membrane translocase subunit Tim14 1 139 1
PTHR12763 HMMPANTHER 1 139 1
1.10.287.110 Gene3D IPR001623 DnaJ domain 41 103 26
SSF46565 SuperFamily IPR001623 DnaJ domain 41 103 25
SignalP-noTM signalp 1 17 212

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.08 Da
Charge 15.00
Isoelectric point 11.25
Molecular weight 15.27 kDa
Number of residues 140
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
9960during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
7595during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
7718during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
11813during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
3827.42during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
7284during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1804.64during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPAC824.06 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC824.06 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC824.06 BioGRID Interaction Datasets
Expression Viewer SPAC824.06 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC824.06 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC824.06 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC824.06 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC824.06 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC824.06 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC824.06 Cell Cycle Data
GEO SPAC824.06 GEO profiles
PInt SPAC824.06 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC824.06 Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene2542780TIM23 translocase complex subunit Tim14
EntrezGene2542780TIM23 translocase complex subunit Tim14
SPD / RIKEN05/05C01Orfeome Localization Data
UniProtKB/SwissProtQ9UT37Mitochondrial import inner membrane translocase subunit tim14
ModBaseQ9UT37Database of comparative protein structure models
STRINGQ9UT37Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593445TIM23 translocase complex subunit Tim14
RefSeq mRNANM_001018878972h- TIM23 translocase complex subunit Tim14 (tim14), mRNA
European Nucleotide ArchiveCAB57336ENA Protein Mapping
European Nucleotide ArchiveCAB57336.1ENA Protein Mapping
UniParcUPI000006C54BUniProt Archive

Literature for tim14

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014