pcm2 (SPAC869.08)

Gene Standard Namepcm2 Characterisation Statusconserved unknown
Systematic IDSPAC869.08 Feature Typeprotein coding
Synonyms Name DescriptionProtein Carboxyl Methyltransferase
Productprotein-L-isoaspartate O-methyltransferase Pcm2 (predicted) Product Size230aa, 25.33 kDa
Genomic Location Chromosome I, 5499280-5497918 (1363nt); CDS:5498965-5498273 (693nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
protein repair3
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationpcm2Δ3815

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologypcm2Δ3095
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR5499280..5498966PMID:21511999
3' UTR5498272..5497918PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01135 Pfam IPR000682 Protein-L-isoaspartate(D-aspartate) O-methyltransferase 9 223 1
PTHR11579 HMMPANTHER IPR000682 Protein-L-isoaspartate(D-aspartate) O-methyltransferase 1 230 1 Gene3D IPR029063 S-adenosyl-L-methionine-dependent methyltransferase 8 225 67
SSF53335 SuperFamily IPR029063 S-adenosyl-L-methionine-dependent methyltransferase 9 203 68
TIGR00080 tigrfam IPR000682 Protein-L-isoaspartate(D-aspartate) O-methyltransferase 9 223 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.14 Da
Charge 0.00
Codon Adpatation Index 0.37
Isoelectric point 6.51
Molecular weight 25.33 kDa
Number of residues 230
Gene Expression

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.18during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
0.53during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
no apparent S. cerevisiae ortholog600
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3421
conserved in vertebrates3396

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withatb2tubulin alpha 2 Positive GeneticPMID:22681890
negative genetic interaction withdbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
positive genetic interaction withdsk1SR protein-specific kinase Dsk1 Positive GeneticPMID:22681890
negative genetic interaction withers1RNA-silencing factor Ers1 Negative GeneticPMID:22681890
positive genetic interaction withhus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
negative genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
negative genetic interaction withppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withvps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
External References
Database Identifier Description
NBRP SPAC869.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC869.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC869.08 BioGRID Interaction Datasets
Expression Viewer SPAC869.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC869.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC869.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC869.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC869.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC869.08 Transcriptome Viewer (Bähler Lab)
GEO SPAC869.08 GEO profiles
PInt SPAC869.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC869.08 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC869.08 Fission yeast phenotypic data & analysis
Cyclebase SPAC869.08.1 Cell Cycle Data
SPD / RIKEN08/08A11Orfeome Localization Data
IntEnz2.1.1.77Integrated relational Enzyme database
Rhea2.1.1.77Annotated reactions database
UniProtKB/SwissProtQ9URZ1Probable protein-L-isoaspartate O-methyltransferase
ModBaseQ9URZ1Database of comparative protein structure models
STRINGQ9URZ1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595011protein-L-isoaspartate O-methyltransferase Pcm2 (predicted)
RefSeq mRNANM_001020442972h- protein-L-isoaspartate O-methyltransferase Pcm2 (predicted) (pcm2), mRNA
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveCAB60018ENA Protein Mapping
European Nucleotide ArchiveCAB60018.1ENA Protein Mapping
UniParcUPI000006B5A9UniProt Archive

Literature for pcm2

Search: Europe PMC or PubMed

Release Version: PomBase:28_55 - 09 Sep 2015