pcm2 (SPAC869.08)


Gene Standard Namepcm2 Characterisation Statusconserved_unknown
Systematic IDSPAC869.08 Feature Typeprotein coding
Synonyms Name DescriptionProtein Carboxyl Methyltransferase
Productprotein-L-isoaspartate O-methyltransferase Pcm2 (predicted) Product Size230aa, 25.33 kDa
Genomic Location Chromosome I, 5499280-5497918 (1363nt); CDS:5498965-5498273 (693nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004719protein-L-isoaspartate (D-aspartate) O-methyltransferase activityISSUniProtKB:Q43209GO_REF:00000011
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmIDAPMID:168233724204
GO:0005829cytosolIDAPMID:168233722319
GO:0005634nucleusIDAPMID:168233722737
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopypcm2ΔNullPECO:0000005, PECO:0000137PMID:236978063751
Microscopypcm2ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopypcm2ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
154992805497918
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01135 Pfam IPR000682 Protein-L-isoaspartate(D-aspartate) O-methyltransferase 9 223 1
PTHR11579 HMMPANTHER IPR000682 Protein-L-isoaspartate(D-aspartate) O-methyltransferase 1 230 1
PTHR11579:SF0 HMMPANTHER 1 230 1
3.40.50.150 Gene3D 8 225 67
SSF53335 SuperFamily S-adenosyl-L-methionine-dependent methyltransferase-like 9 203 68
TIGR00080 tigrfam IPR000682 Protein-L-isoaspartate(D-aspartate) O-methyltransferase 9 223 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.14 Da
Charge 0.00
Isoelectric point 6.51
Molecular weight 25.33 kDa
Number of residues 230
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.18during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.53during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog601
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in bacteria1002
conserved in metazoa3428
conserved in vertebrates3402
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
dsk1SR protein-specific kinase Dsk1 Positive GeneticPMID:22681890
dbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
ppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
rpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:22681890
ers1RNA-silencing factor Ers1 Negative GeneticPMID:22681890
hus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
xap5xap-5-like protein Negative GeneticPMID:24957674
atb2tubulin alpha 2 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC869.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC869.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC869.08 BioGRID Interaction Datasets
Expression Viewer SPAC869.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC869.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC869.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC869.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC869.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC869.08 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC869.08 Cell Cycle Data
GEO SPAC869.08 GEO profiles
PInt SPAC869.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC869.08 Peptides identified in tandem mass spectrometry proteomics experiments
IntEnz2.1.1.77Integrated relational Enzyme database
Rhea2.1.1.77Annotated reactions database
SPD / RIKEN08/08A11Orfeome Localization Data
UniProtKB/SwissProtQ9URZ1Probable protein-L-isoaspartate O-methyltransferase
ModBaseQ9URZ1Database of comparative protein structure models
STRINGQ9URZ1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595011protein-L-isoaspartate O-methyltransferase Pcm2 (predicted)
RefSeq mRNANM_001020442972h- protein-L-isoaspartate O-methyltransferase Pcm2 (predicted) (pcm2), mRNA
European Nucleotide ArchiveCAB60018.1ENA Protein Mapping
UniParcUPI000006B5A9UniProt Archive

Literature for pcm2

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014