rad24 (SPAC8E11.02c)

Gene Standard Namerad24 ChromosomeI
Systematic IDSPAC8E11.02c Gene Start3384190
Synonymssam4 Gene End3385685
Product14-3-3 protein Rad24 Gene Length1496
Feature Typeprotein coding CDS Start3384268
Name Description CDS End3385487
Characterisation Statuspublished CDS Length1220

Ensembl Gene Location
  1. alp31
  2. SPAC8E11.06
  3. SPAC8E11.05c
  4. SPAC8E11.04c
  5. SPNCRNA.904
  6. dmc1
  7. rad24
  8. SPNCRNA.905
  9. SPAC8E11.01c
  10. sec17
  11. SPAC959.03c
  12. SPAC959.11
  13. omh6
  14. sen15
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000011abnormal cell cycle progressionnot recorded (overexpression)unknownPMID:12237855
FYPO:0000023small cellrad24delta (deletion)deletionPMID:8036497
FYPO:0000266sensitive to DNA damaging agentscompetitive growth assay evidencerad24delta (deletion)deletionPMID:20537132
FYPO:0000268sensitive to UVCell growth assayrad24delta (deletion)deletionPECO:0000012PMID:19584544
competitive growth assay evidencerad24delta (deletion)deletionPMID:20537132
FYPO:0002060viable vegetative cell populationMicroscopyrad24delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0004864protein phosphatase inhibitor activityIGIcdc25PMID:9832516
GO:0005515protein bindingIPIclp1PMID:16085489
has_direct_input PR:000027543IPIste11PMID:11702950
IPIcid13PMID:12062100
IPIcdc25PMID:9774107
IPIchk1PMID:12415000
IPImei2PMID:10523629
IPIbyr2PMID:12242289
GO:0046982protein heterodimerization activityIPIrad25PMID:12242289
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0000077DNA damage checkpointIMPPMID:8036497
GO:0000122negative regulation of transcription from RNA polymerase II promoterIMPPMID:11702950
GO:0006281DNA repairIEAUniProtKB:KW-0234GO_REF:0000037
GO:0007126meiosisIMPPMID:10788621
GO:0009267cellular response to starvationIMPPMID:19584544
GO:0010515negative regulation of induction of conjugation with cellular fusionIMPPMID:19584544
GO:0031031positive regulation of septation initiation signaling cascadeIGIclp1PMID:16085489
GO:0031565cytokinesis checkpointIMPPMID:15265986
GO:0034613cellular protein localization
has_input byr2IMPPMID:12242289
has_input clp1IMPPMID:16085489
GO:0034644cellular response to UVIMPPMID:19584544
GO:0043433negative regulation of sequence-specific DNA binding transcription factor activityIMPPMID:11702950
GO:0051038negative regulation of transcription during meiosisIMPPMID:11702950
GO:0071277cellular response to calcium ionIMPPMID:19584544
GO:0071472cellular response to salt stressIMPPMID:19584544
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0005737cytoplasmIDAPMID:16085489
GO:0005829cytosolIDAPMID:16823372
GO:0032153cell division siteIDAPMID:16823372
exists_during mitotic cell cycleIDAPMID:16085489
GO:0044732mitotic spindle pole bodyIDAPMID:16823372
GO:0051233spindle midzone
exists_during mitotic cell cycleIDAPMID:16085489
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
133841903384337
233847453385685

References

Region Start End Reference
three_prime_UTR33854883385685PMID:21511999
three_prime_UTR33854883385685PMID:21511999
five_prime_UTR33841903384267PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00244 IPR023410 Pfam 14-3-3 protein 6 238 2
SM00101 IPR023410 SMART 14-3-3 domain 6 247 2
PS00796 IPR023409 Prosite Patterns 14-3-3 protein 44 54 2
PS00797 IPR023409 Prosite Patterns 14-3-3 protein 216 235 2
G3DSA:1.20.190.20 IPR023410 Gene3D 4 234 2
SSF48445 IPR023410 SuperFamily 5 236 2
PIRSF000868 IPR000308 PIRSF 14-3-3 protein 4 246 2
PTHR18860 IPR000308 hmmpanther 14-3-3 protein 4 255 2
PTHR18860:SF0 hmmpanther 4 255 2
Coil ncoils Rabaptin coiled-coil domain 12 33 974
PR00305 IPR000308 PRINTS 14-3-3 protein 207 236 2
PR00305 IPR000308 PRINTS 14-3-3 protein 38 67 2
PR00305 IPR000308 PRINTS 14-3-3 protein 87 111 2
PR00305 IPR000308 PRINTS 14-3-3 protein 118 140 2
PR00305 IPR000308 PRINTS 14-3-3 protein 153 179 2
PR00305 IPR000308 PRINTS 14-3-3 protein 180 206 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.42 Da
Charge -16.50
Isoelectric point 4.37
Molecular weight 30.08 kDa
Number of residues 270
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide128664.86PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide61836.74PMID:23101633
RNA levelexperimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide89PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide9.7PMID:23101633
Genome Organisation
DescriptionQualifierReference
co-expressed with adjacent tandem gene
Pathway
DescriptionQualifierReference
negative regulator of the Ras1-Byr2 signalling pathway, acting downstream of Ras1 PMID:12242289
Species Distribution
DescriptionQualifierReference
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Phenotypic EnhancementPMID:18385517
rad13DNA repair nuclease Rad13 Phenotypic EnhancementPMID:9613582
orc1origin recognition complex subunit Orc1 Phenotypic EnhancementPMID:18667534
prz1calcineurin responsive transcription factor Prz1 Dosage RescuePMID:22496451
sre2membrane-tethered transcription factor (predicted) Negative GeneticPMID:21504829
rst2transcription factor Rst2 Dosage RescuePMID:22496451
ran1serine/threonine protein kinase Ran1 Synthetic LethalityPMID:11818066
Dosage Rescue
hsp9heat shock protein Hsp9 Dosage RescuePMID:22182414
cdc20DNA polymerase epsilon catalytic subunit Pol2 Phenotypic EnhancementPMID:18667534
cut14condensin complex subunit Cut14 Synthetic LethalityPMID:22645654
dbr1RNA lariat debranching enzyme Dbr1 Synthetic Growth DefectPMID:16049013
chk1Chk1 protein kinase Synthetic RescuePMID:16816416
ste11transcription factor Ste11 Phenotypic SuppressionPMID:11702950
dis2serine/threonine protein phosphatase PP1 Synthetic Growth DefectPMID:21965289
mis4adherin, cohesin loading factor Mis4 Negative GeneticPMID:23050226
ras1GTPase Ras1 Phenotypic SuppressionPMID:12242289
SPBC4B4.04translation initiation factor eIF2A (predicted) Negative GeneticPMID:22681890
cdc25M phase inducer phosphatase Cdc25 Phenotypic SuppressionPMID:11166180
Dosage RescuePMID:9832516
rad2514-3-3 protein Rad25 Dosage RescuePMID:22496451
cut9anaphase-promoting complex subunit Cut9 Dosage RescuePMID:15507118
Dosage RescuePMID:12653962
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
cds1replication checkpoint kinase Cds1 Phenotypic EnhancementPMID:9832516
prp12U2 snRNP-associated protein Sap130 Synthetic Growth DefectPMID:16049013
cdc28ATP-dependent RNA helicase Cdc28 Synthetic Growth DefectPMID:16049013
cdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Synthetic RescuePMID:16816416
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
rad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Dosage RescuePMID:16930478
emc1ER membrane protein complex subunit Emc1 (predicted) Negative GeneticPMID:22681890
srk1MAPK-activated protein kinase Srk1 Phenotypic SuppressionPMID:15629716
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
mei2RNA-binding protein involved in meiosis Mei2 Synthetic RescuePMID:10788621
Dosage LethalityPMID:11818066
rad57RecA family ATPase Rad57/Rhp57 Negative GeneticPMID:22681890
spp27RNA polymerase I upstream activation factor complex subunit Spp27 Negative GeneticPMID:22681890
pka1cAMP-dependent protein kinase catalytic subunit Pka1 Dosage RescuePMID:19584544
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23394829
Reconstituted ComplexPMID:16085489
Co-localization
Affinity Capture-WesternPMID:22918952
Affinity Capture-MSPMID:23297348
rpt219S proteasome regulatory subunit Rpt2 Affinity Capture-WesternPMID:20231270
plc1phosphoinositide phospholipase C Plc1 Two-hybridPMID:9613582
Affinity Capture-Western
prz1calcineurin responsive transcription factor Prz1 Affinity Capture-WesternPMID:22496451
SPAC25G10.08translation initiation factor eIF3b (p84) Affinity Capture-WesternPMID:20231270
cdc22ribonucleoside reductase large subunit Cdc22 Affinity Capture-WesternPMID:20231270
scw1RNA-binding protein Scw1 Affinity Capture-MSPMID:23394829
cyk3cytokinesis protein Cyk3 (predicted) Affinity Capture-MSPMID:20603077
rpn50219S proteasome regulatory subunit Rpn502 Affinity Capture-WesternPMID:20231270
mts419S proteasome regulatory subunit Mts4 Affinity Capture-WesternPMID:20231270
mph1dual specificity protein kinase Mph1 Affinity Capture-MSPMID:23394829
mug72oxidoreductase (predicted) Affinity Capture-MSPMID:23394829
rpn619S proteasome regulatory subunit Rpn6 (predicted) Affinity Capture-WesternPMID:20231270
hsp9heat shock protein Hsp9 Affinity Capture-WesternPMID:22182414
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
ago1argonaute Affinity Capture-WesternPMID:17043360
chk1Chk1 protein kinase Affinity Capture-WesternPMID:12415000
Two-hybridPMID:10090724
Affinity Capture-Western
Two-hybridPMID:17502373
Affinity Capture-WesternPMID:15585577
Affinity Capture-WesternPMID:20639859
ipp1inorganic pyrophosphatase (predicted) Affinity Capture-MSPMID:23394829
ase1antiparallel microtubule cross-linking factor Ase1 Affinity Capture-MSPMID:23394829
SPAC16E8.08sequence orphan Affinity Capture-MSPMID:23394829
ste11transcription factor Ste11 Affinity Capture-WesternPMID:11702950
Reconstituted Complex
cid13poly(A) polymerase Cid13 Affinity Capture-MSPMID:12062100
cdc11SIN component scaffold protein, centriolin ortholog Cdc11 Affinity Capture-MSPMID:23394829
SPAC3G9.05GTPase activating protein (predicted) Affinity Capture-MSPMID:23394829
rae1RNA export factor, nucleoporin Rae1 Affinity Capture-WesternPMID:20231270
Reconstituted Complex
cdc25M phase inducer phosphatase Cdc25 Affinity Capture-WesternPMID:20231270
Affinity Capture-WesternPMID:9923681
Reconstituted ComplexPMID:9774107
Affinity Capture-WesternPMID:10090724
Affinity Capture-WesternPMID:10523629
Reconstituted Complex
Affinity Capture-WesternPMID:22182414
rad2514-3-3 protein Rad25 Affinity Capture-WesternPMID:20231270
Affinity Capture-WesternPMID:12242289
slm9hira protein Slm9 Affinity Capture-MSPMID:20976105
rpn50119S proteasome regulatory subunit Rpn501 Affinity Capture-WesternPMID:20231270
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Affinity Capture-WesternPMID:20603077
Affinity Capture-WesternPMID:20231270
rng2IQGAP Affinity Capture-MSPMID:20603077
ppk25serine/threonine protein kinase Ppk25 (predicted) Affinity Capture-MSPMID:23394829
ssp1serine/threonine protein kinase Ssp1 Affinity Capture-MSPMID:19371376
tif32translation initiation factor eIF3a Affinity Capture-WesternPMID:20231270
nak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23394829
Affinity Capture-MSPMID:23462181
rgf1RhoGEF for Rho1, Rgf1 Affinity Capture-MSPMID:23394829
rad51RecA family recombinase Rad51/Rhp51 Reconstituted ComplexPMID:20231270
Affinity Capture-Western
tif471translation initiation factor eIF4G Affinity Capture-WesternPMID:20231270
rik1silencing protein Rik1 Affinity Capture-MSPMID:17114925
rad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Co-localizationPMID:16930478
ssa2heat shock protein Ssa2 (predicted) Affinity Capture-WesternPMID:20231270
mei2RNA-binding protein involved in meiosis Mei2 Two-hybridPMID:10788621
Affinity Capture-WesternPMID:11818066
Two-hybrid
lrp1leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
Affinity Capture-MSPMID:23394829
byr2MAP kinase kinase kinase Byr2 Affinity Capture-WesternPMID:12242289
Reconstituted Complex
dss1mRNA export protein Dss1 Reconstituted ComplexPMID:20231270
Affinity Capture-Western
ssa1heat shock protein Ssa1 (predicted) Affinity Capture-WesternPMID:20231270
raf2Rik1-associated factor Raf2 Affinity Capture-MSPMID:17114925
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External References
Database Identifier Description
NBRP SPAC8E11.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC8E11.02c Retrieval of eukaryotic orthologs
BioGrid SPAC8E11.02c BioGRID Interaction Datasets
Bähler Lab SPAC8E11.02c Cell Cycle Expression Profile
Bähler Lab SPAC8E11.02c Meiosis/Sporulation Expression Profies
Bähler Lab SPAC8E11.02c Pheromone response/mating expression profiles
Bähler Lab SPAC8E11.02c Environmental stress expression profiles
Bähler Lab SPAC8E11.02c Bähler Lab Transcriptome Viewer
Cyclebase SPAC8E11.02c Cell Cycle Data
PInt SPAC8E11.02c Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAC8E11.02c Entrez Gene
WikiGene254202914-3-3 protein Rad24
Uniprot_genenameRAD24
EntrezGene254202914-3-3 protein Rad24
SPD / RIKEN40/40D06Orfeome Localization Data
UniProtKB/SwissProtP42656DNA damage checkpoint protein rad24
ModBaseP42656Database of comparative protein structure models
Pfam Protein DomainsP42656Pfam Domain Arrangement
RefSeq PeptideNP_59416714-3-3 protein Rad24
RefSeq mRNANM_001019591972h- 14-3-3 protein Rad24 (rad24), mRNA
European Nucleotide ArchiveBAA24800ENA Protein Mapping
European Nucleotide ArchiveBAA28672ENA Protein Mapping
European Nucleotide ArchiveCAA17023ENA Protein Mapping
European Nucleotide ArchiveCAA55795ENA Protein Mapping
UniParcUPI000013301CUniProt Archive

Literature for rad24
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013