rad24 (SPAC8E11.02c)


Gene Standard Namerad24 Characterisation Statuspublished
Systematic IDSPAC8E11.02c Feature Typeprotein coding
Synonymsanr5, sam4 Name Description
Product14-3-3 protein Rad24 Product Size270aa, 30.08 kDa
Genomic Location Chromosome I, 3384190-3385685 (1496nt); CDS:3384268-3385487 (1220nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005515protein bindingIPIbyr2PMID:12242289861
IPIchk1PMID:12415000
IPIclp1PMID:16085489
IPIcdc25PMID:9774107
IPIcid13PMID:12062100
binds transcription factor ste11 phosphorylated 1 (fission yeast)IPIste11PMID:11702950
IPImei2PMID:10523629
GO:0004864protein phosphatase inhibitor activityIGIcdc25PMID:98325164
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0034613cellular protein localization552
localizes clp1IMPPMID:16085489
localizes byr2IMPPMID:12242289
GO:0031565cytokinesis checkpointIMPPMID:152659863
GO:0007126meiotic nuclear divisionIMPPMID:10788621110
GO:0044773mitotic DNA damage checkpointIMPPMID:803649743
GO:0010515negative regulation of induction of conjugation with cellular fusionIMPPMID:1958454412
GO:0043433negative regulation of sequence-specific DNA binding transcription factor activityIMPPMID:117029504
GO:0000122negative regulation of transcription from RNA polymerase II promoterIMPPMID:1170295078
GO:0031031positive regulation of septation initiation signalingIGIclp1PMID:160854899
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032153cell division siteIDAPMID:16823372296
during mitotic cell cycleIDAPMID:16085489
GO:0005737cytoplasmIDAPMID:160854894201
GO:0005829cytosolIDAPMID:168233722316
GO:0044732mitotic spindle pole bodyIDAPMID:16823372214
GO:0051233spindle midzone13
during mitotic cell cycleIDAPMID:16085489
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002620normal growth on trichostatin ACell growth assayrad24ΔNullPECO:0000005, PECO:0000201, PECO:0000142PMID:23861937110
FYPO:0001098sensitive to 4-nitroquinoline N-oxide159
expressivity FYPO_EXT:0000003Cell growth assayrad24ΔNullPECO:0000137, PECO:0000005PMID:19264558
FYPO:0000268sensitive to UV during vegetative growthCell growth assaysam4 (Q70->ochre)PECO:0000137PMID:19584544118
Cell growth assayrad24ΔNullPECO:0000137PMID:19584544
competitive growth assay evidencerad24ΔNullPMID:20537132
FYPO:0001701sensitive to bortezomib256
expressivity FYPO_EXT:0000002Cell growth assayrad24ΔNullPECO:0000005, PECO:0000137PMID:21760946
FYPO:0000098sensitive to calciumCell growth assaysam4 (Q70->ochre)PECO:0000137PMID:195845448
FYPO:0000085sensitive to camptothecin211
expressivity FYPO_EXT:0000003Cell growth assayrad24ΔNullPECO:0000137, PECO:0000005PMID:19264558
FYPO:0003559sensitive to doxorubicin91
expressivity FYPO_EXT:0000003Cell growth assayrad24ΔNullPECO:0000137, PECO:0000142PMID:23365689
FYPO:0000081sensitive to high osmolarityCell growth assaysam4 (Q70->ochre)PECO:0000137PMID:195845448
FYPO:0000088sensitive to hydroxyurea511
expressivity FYPO_EXT:0000003Cell growth assayrad24ΔNullPECO:0000137, PECO:0000005PMID:19264558
FYPO:0000089sensitive to methyl methanesulfonate212
expressivity FYPO_EXT:0000003Cell growth assayrad24ΔNullPECO:0000137, PECO:0000005PMID:19264558
FYPO:0000271sensitive to salt stressCell growth assaysam4 (Q70->ochre)PECO:0000137PMID:1958454482
FYPO:0002617sensitive to sodium butyrate93
expressivity FYPO_EXT:0000002Cell growth assayrad24ΔNullPECO:0000201, PECO:0000005, PECO:0000142PMID:23861937
FYPO:0000086sensitive to tacrolimus81
expressivity FYPO_EXT:0000002Cell growth assayrad24ΔNullPECO:0000200, PECO:0000005, PECO:0000142PMID:21850271
Cell growth assayrad24+ (wild type)OverexpressionPECO:0000005, PECO:0000201PMID:22496451
FYPO:0000115sensitive to valproic acid155
expressivity FYPO_EXT:0000003Cell growth assayrad24ΔNullPECO:0000201, PECO:0000005, PECO:0000142PMID:23861937
FYPO:0002060viable vegetative cell populationMicroscopyrad24ΔNullPECO:0000005, PECO:0000137PMID:236978063730
Microscopyrad24ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000011abnormal cell cyclenot recorded (overexpression)PMID:12237855901
FYPO:0002894decreased transcription during cellular response to calcium ion6
affecting SO:0001865Reporter gene assayrad24+ (wild type)OverexpressionPECO:0000126PMID:22496451
affecting SO:0001843Reporter gene assayrad24+ (wild type)OverexpressionPECO:0000126PMID:22496451
FYPO:0001122elongated vegetative cellMicroscopyrad24+ (wild type)OverexpressionPMID:12237855657
FYPO:0002243increased acid phosphatase activitySubstance quantification evidenceanr5-13 (L225S)Not specifiedPMID:139451010
FYPO:0000761increased conjugation frequencyMicroscopysam4 (Q70->ochre)PECO:0000137PMID:195845449
FYPO:0001043increased mating efficiencyCell growth assayrad24ΔNullPECO:0000127PMID:1516613817
FYPO:0002895increased transcription during cellular response to calcium ion2
affecting SO:0001865Reporter gene assayrad24ΔNullPECO:0000126PMID:22496451
FYPO:0000023small cellMicroscopyrad24ΔNullPMID:803649770
FYPO:0002106viable stubby vegetative cell52
penetrance FYPO_EXT:0000001Microscopyrad24ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in prp2 U2AF large subunit (U2AF-59) PMID:23754748
FYPO affected by mutation in prz1 calcineurin responsive transcription factor Prz1 PMID:22496451
FYPO affected by mutation in slu7 splicing factor Slu7 PMID:23754748
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
133841903384337
233847453385685

UTRs

Region Coordinates Reference
five_prime_UTR3384190..3384267PMID:21511999
three_prime_UTR3385488..3385685PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00244 Pfam IPR023410 14-3-3 domain 6 238 2
SM00101 SMART IPR023410 14-3-3 domain 6 247 2
PS00797 Prosite Patterns IPR023409 14-3-3 protein, conserved site 216 235 2
PS00796 Prosite Patterns IPR023409 14-3-3 protein, conserved site 44 54 2
PTHR18860 HMMPANTHER IPR000308 14-3-3 protein 1 270 2
3iquA00 Gene3D IPR023410 14-3-3 domain 4 234 2
SSF48445 SuperFamily IPR023410 14-3-3 domain 5 236 2
PIRSF000868 PIRSF IPR000308 14-3-3 protein 4 246 2
Coil ncoils Rabaptin coiled-coil domain 12 33 968
PR00305 PRINTS IPR000308 14-3-3 protein 118 140 2
PR00305 PRINTS IPR000308 14-3-3 protein 38 67 2
PR00305 PRINTS IPR000308 14-3-3 protein 180 206 2
PR00305 PRINTS IPR000308 14-3-3 protein 207 236 2
PR00305 PRINTS IPR000308 14-3-3 protein 87 111 2
PR00305 PRINTS IPR000308 14-3-3 protein 153 179 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.42 Da
Charge -16.50
Isoelectric point 4.37
Molecular weight 30.08 kDa
Number of residues 270
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS212PMID:217125471669
present during mitotic M phase
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
459410during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
446427during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
445942during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
448301during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
128664.86during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
457536during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
61836.74during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
89during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
9.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
heteromeric(2) PMID:1224228942
Genome Organisation
DescriptionQualifierReferenceCount
co-expressed with adjacent tandem gene2
Pathway
DescriptionQualifierReferenceCount
negative regulator of the Ras1-Byr2 signalling pathway, acting downstream of Ras1 PMID:122422891
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ras1GTPase Ras1 Phenotypic SuppressionPMID:12242289
cdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Synthetic RescuePMID:16816416
mei2RNA-binding protein involved in meiosis Mei2 Synthetic RescuePMID:10788621
Dosage LethalityPMID:11818066
mis4adherin, cohesin loading factor Mis4 Negative GeneticPMID:23050226
cdc25M phase inducer tyrosine phosphatase Cdc25 Phenotypic SuppressionPMID:11166180
Dosage RescuePMID:9832516
hsp9heat shock protein Hsp9 Dosage RescuePMID:22182414
clp1Cdc14-related protein phosphatase Clp1/Flp1 Phenotypic EnhancementPMID:18385517
cdc20DNA polymerase epsilon catalytic subunit Pol2 Phenotypic EnhancementPMID:18667534
cut14condensin complex subunit Cut14 Synthetic LethalityPMID:22645654
rad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Dosage RescuePMID:16930478
rad2514-3-3 protein Rad25 Dosage RescuePMID:22496451
chk1Chk1 protein kinase Synthetic RescuePMID:16816416
msa1RNA-binding protein Msa1 Dosage RescuePMID:15166138
rad57RecA family ATPase Rad57/Rhp57 Negative GeneticPMID:22681890
gsf1transcription factor, zf-fungal binuclear cluster type Gsf1 Dosage RescuePMID:23311928
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
ste11transcription factor Ste11 Phenotypic SuppressionPMID:11702950
sre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
orc1origin recognition complex subunit Orc1 Phenotypic EnhancementPMID:18667534
pka1cAMP-dependent protein kinase catalytic subunit Pka1 Dosage RescuePMID:19584544
cds1replication checkpoint kinase Cds1 Phenotypic EnhancementPMID:9832516
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
dis2serine/threonine protein phosphatase PP1 subfamily, Dis2 Synthetic Growth DefectPMID:21965289
dbr1RNA lariat debranching enzyme Dbr1 Synthetic Growth DefectPMID:16049013
cut9anaphase-promoting complex subunit Cut9 Dosage RescuePMID:12653962
Dosage RescuePMID:15507118
srk1MAPK-activated protein kinase Srk1 Phenotypic SuppressionPMID:15629716
spp27RNA polymerase I upstream activation factor complex subunit Spp27 Negative GeneticPMID:22681890
emc1ER membrane protein complex subunit Emc1 (predicted) Negative GeneticPMID:22681890
prp12U2 snRNP-associated protein Sap130 Synthetic Growth DefectPMID:16049013
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
cdc28ATP-dependent RNA helicase Cdc28 Synthetic Growth DefectPMID:16049013
ran1serine/threonine protein kinase Ran1 Dosage RescuePMID:11818066
Synthetic Lethality
prz1calcineurin responsive transcription factor Prz1 Dosage RescuePMID:22496451
SPBC4B4.04translation initiation factor eIF2A (predicted) Negative GeneticPMID:22681890
rad13DNA repair nuclease Rad13 Phenotypic EnhancementPMID:9613582
rst2transcription factor Rst2 Dosage RescuePMID:22496451
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
tif32translation initiation factor eIF3a Affinity Capture-WesternPMID:20231270
ase1antiparallel microtubule cross-linking factor Ase1 Affinity Capture-MSPMID:23394829
ppk25serine/threonine protein kinase Ppk25 (predicted) Affinity Capture-MSPMID:23394829
rng2IQGAP Affinity Capture-MSPMID:20603077
mph1dual specificity protein kinase Mph1 Affinity Capture-MSPMID:23394829
rad2414-3-3 protein Rad24 Affinity Capture-WesternPMID:20231270
Affinity Capture-WesternPMID:12242289
ssa2heat shock protein Ssa2 Affinity Capture-WesternPMID:20231270
ago1argonaute Affinity Capture-WesternPMID:17043360
mts419S proteasome regulatory subunit Mts4 Affinity Capture-WesternPMID:20231270
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
cdc22ribonucleoside reductase large subunit Cdc22 Affinity Capture-WesternPMID:20231270
mei2RNA-binding protein involved in meiosis Mei2 Two-hybridPMID:10788621
Affinity Capture-WesternPMID:11818066
Two-hybrid
byr2MAP kinase kinase kinase Byr2 Affinity Capture-WesternPMID:12242289
Reconstituted Complex
cdc25M phase inducer tyrosine phosphatase Cdc25 Affinity Capture-WesternPMID:22182414
Affinity Capture-WesternPMID:20231270
Reconstituted ComplexPMID:9774107
Affinity Capture-WesternPMID:10090724
Affinity Capture-WesternPMID:10523629
Reconstituted Complex
Affinity Capture-WesternPMID:9923681
hsp9heat shock protein Hsp9 Affinity Capture-WesternPMID:22182414
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
Affinity Capture-MSPMID:23394829
Reconstituted ComplexPMID:16085489
Affinity Capture-WesternPMID:22918952
rgf1RhoGEF for Rho1, Rgf1 Affinity Capture-MSPMID:23394829
rpn619S proteasome regulatory subunit Rpn6 (predicted) Affinity Capture-WesternPMID:20231270
ssp1Ca2+/calmodulin-dependent (CaMMK)-like protein kinase Ssp1 Affinity Capture-MSPMID:19371376
SPAC16E8.08sequence orphan Affinity Capture-MSPMID:23394829
rad2514-3-3 protein Rad25 Affinity Capture-WesternPMID:20231270
Affinity Capture-WesternPMID:12242289
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
rik1silencing protein Rik1 Affinity Capture-MSPMID:17114925
rpn50219S proteasome regulatory subunit Rpn502 Affinity Capture-WesternPMID:20231270
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
slm9hira protein Slm9 Affinity Capture-MSPMID:20976105
rae1RNA export factor, nucleoporin Rae1 Reconstituted ComplexPMID:20231270
Affinity Capture-Western
cdc11SIN component scaffold protein, centriolin ortholog Cdc11 Affinity Capture-MSPMID:23394829
Affinity Capture-WesternPMID:22419817
cyk3cytokinesis protein Cyk3 (predicted) Affinity Capture-MSPMID:20603077
chk1Chk1 protein kinase Affinity Capture-WesternPMID:10090724
Two-hybrid
Two-hybridPMID:17502373
Affinity Capture-WesternPMID:20639859
Affinity Capture-WesternPMID:12415000
Affinity Capture-WesternPMID:15585577
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
SPAC25G10.08translation initiation factor eIF3b (p84) Affinity Capture-WesternPMID:20231270
rpn15proteasome regulatory particle, lid subcomplex subunit Rpn15/Dss11 Affinity Capture-WesternPMID:20231270
Reconstituted Complex
rpt219S proteasome regulatory subunit Rpt2 Affinity Capture-WesternPMID:20231270
scw1RNA-binding protein Scw1 Affinity Capture-MSPMID:23394829
ssa1heat shock protein Ssa1 (predicted) Affinity Capture-WesternPMID:20231270
nak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23394829
Affinity Capture-MSPMID:23462181
cid13poly(A) polymerase Cid13 Affinity Capture-MSPMID:12062100
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Affinity Capture-WesternPMID:20231270
Affinity Capture-WesternPMID:20603077
mug72oxidoreductase (predicted) Affinity Capture-MSPMID:23394829
ipp1inorganic pyrophosphatase ipp1 (predicted) Affinity Capture-MSPMID:23394829
spa2GTPase activating protein Spa2 (predicted) Affinity Capture-MSPMID:23394829
rad51RecA family recombinase Rad51/Rhp51 Affinity Capture-WesternPMID:20231270
Reconstituted Complex
sog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23394829
Affinity Capture-MSPMID:23462181
prz1calcineurin responsive transcription factor Prz1 Affinity Capture-WesternPMID:22496451
ste11transcription factor Ste11 Affinity Capture-WesternPMID:11702950
Reconstituted Complex
rpn50119S proteasome regulatory subunit Rpn501 Affinity Capture-WesternPMID:20231270
tif471translation initiation factor eIF4G Affinity Capture-WesternPMID:20231270
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
raf2Rik1-associated factor Raf2 Affinity Capture-MSPMID:17114925
plc1phosphoinositide phospholipase C Plc1 Affinity Capture-WesternPMID:9613582
Two-hybrid
External References
Database Identifier Description
NBRP SPAC8E11.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC8E11.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC8E11.02c BioGRID Interaction Datasets
Expression Viewer SPAC8E11.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC8E11.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC8E11.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC8E11.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC8E11.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC8E11.02c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC8E11.02c Cell Cycle Data
GEO SPAC8E11.02c GEO profiles
PInt SPAC8E11.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC8E11.02c Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene254202914-3-3 protein Rad24
EntrezGene254202914-3-3 protein Rad24
SPD / RIKEN40/40D06Orfeome Localization Data
UniProtKB/SwissProtP42656DNA damage checkpoint protein rad24
ModBaseP42656Database of comparative protein structure models
STRINGP42656Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_59416714-3-3 protein Rad24
RefSeq mRNANM_001019591972h- 14-3-3 protein Rad24 (rad24), mRNA
European Nucleotide ArchiveAB010899ENA EMBL mapping
European Nucleotide ArchiveBAA24800ENA Protein Mapping
European Nucleotide ArchiveBAA28672ENA Protein Mapping
European Nucleotide ArchiveBAA28672.1ENA Protein Mapping
European Nucleotide ArchiveCAA17023ENA Protein Mapping
European Nucleotide ArchiveCAA17023.1ENA Protein Mapping
European Nucleotide ArchiveCAA55795ENA Protein Mapping
European Nucleotide ArchiveCAA55795.1ENA Protein Mapping
ReactomeREACT_202295
ReactomeREACT_213398
UniParcUPI000013301CUniProt Archive

Literature for rad24

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014