rlc1 (SPAC926.03)


Gene Standard Namerlc1 Characterisation Statuspublished
Systematic IDSPAC926.03 Feature Typeprotein coding
Synonyms Name Description
Productmyosin II regulatory light chain Rlc1 Product Size184aa, 20.00 kDa
Genomic Location Chromosome I, 3887243-3888497 (1255nt); CDS:3887570-3888124 (555nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
calcium ion binding39
Annotation ExtensionEvidenceWith/FromReference
myosin binding4
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitotic actomyosin contractile ring assembly27
Annotation ExtensionEvidenceWith/FromReference
negative regulation of septation initiation signaling17
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actomyosin contractile ring39
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
myosin II complex4
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencyrlc1Δ275
sensitive to 5-fluorouracilrlc1Δ307
slow vegetative cell population growthrlc1Δ367
viable vegetative cell population3850
rlc1Δ

Cell Phenotype

Term NameGenotypesCount
abnormal actomyosin contractile ring morphologyrlc1Δ7
abolished protein localization to actomyosin contractile ring13
affects localization of chs2rlc1Δ
abolished protein serine phosphorylation during vegetative growth10
affecting rlc1rlc1-SA (S35A, S36A)
decreased protein localization to actomyosin contractile ring16
affects localization of shk1rlc1Δ
normal protein localization to cell tip24
affecting shk1rlc1Δ
viable vegetative cell, abnormal cell shape, normal cell sizerlc1Δ215

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
inviable vegetative cell populationrlc1Δ, pxl1Δ
slow vegetative cell population growthrlc1Δ, pxl1Δ

Cell Phenotype

Term NameGenotypes
cutase1Δ, rlc1-SA (S35A, S36A)
inviable mononucleate monoseptate vegetative cellase1Δ, rlc1-SA (S35A, S36A)
septation following abnormal mitotic chromosome segregationase1Δ, rlc1-SA (S35A, S36A)
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in rlc1 myosin II regulatory light chain Rlc1
FYPO localization affected by mutation in cdc4 myosin II light chain
FYPO localization affected by mutation in rng2 IQGAP
GO localized by cdc4 myosin II light chain
GO localized by rng2 IQGAP
GO substrate of shk1 PAK-related kinase Shk1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3887243..3888497
mRNA3887243..3888497
5' UTR3887243..3887569PMID:21511999
CDS3887570..3888124
3' UTR3888125..3888497PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00036 Pfam IPR002048 EF-hand domain 49 77 2
SM00054 SMART IPR002048 EF-hand domain 49 77 9
SM00054 SMART IPR002048 EF-hand domain 118 146 9
PS50222 Prosite Profiles IPR002048 EF-hand domain 45 80 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 114 149 20
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 58 70 12
PTHR23049 HMMPANTHER 16 184 1
PTHR23049:SF34 HMMPANTHER 16 184 1
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 116 178 20
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 35 108 20
SSF47473 SuperFamily IPR011992 EF-hand domain pair 42 177 21

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276

Protein Properties

Ave. residue weight 108.68 Da
Charge -5.00
Codon Adaptation Index 0.43
Isoelectric point 4.70
Molecular weight 20.00 kDa
Number of residues 184
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS35
present during mitotic M phaseS36
S35, S36, S183
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S35 PMID:21712547
present during mitotic M phase experimental evidence S36 PMID:21712547
IDA S35 PMID:25720772
IDA S183 PMID:25720772
IDA S36 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for rlc1 (SPAC926.03)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8532during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8300during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
9297during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
9600during GO:0072690PECO:0000012,
PECO:0000005
single cellexperimental evidencePMID:16224022
2550.36during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
4481.24during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.84during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC926.03 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bymyo2myosin II heavy chain Affinity Capture-WesternPMID:15504913
affinity captured bymyp2myosin II heavy chain Myo3 Affinity Capture-WesternPMID:11069761
affinity captured bypxl1paxillin-like protein Pxl1 Affinity Capture-WesternPMID:18256290
binds DNA-binding domain construct withmyp2myosin II heavy chain Myo3 Two-hybridPMID:26771498
affinity capturesmyp2myosin II heavy chain Myo3 Affinity Capture-WesternPMID:11069761
affinity capturescdc4myosin II light chain Affinity Capture-WesternPMID:11056543
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:19570908
co-purifies withmyo2myosin II heavy chain Co-purificationPMID:19570908
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC926.03 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic Growth DefectPMID:19139265
synthetic growth defect withimp2contractile ring protein Imp2 Synthetic Growth DefectPMID:19139265
synthetic growth defect withpxl1paxillin-like protein Pxl1 Synthetic Growth DefectPMID:18256290
synthetic growth defect withcdc12formin Cdc12 Synthetic Growth DefectPMID:24115772
synthetic growth defect withrng9contractile ring myosin V regulator Rng9 Synthetic Growth DefectPMID:24798735
synthetic growth defect withrng8contractile ring myosin V regulator Rng8 Synthetic Growth DefectPMID:24798735
synthetic growth defect withmyo51myosin type V Synthetic Growth DefectPMID:24798735
synthetic lethal withfor3formin For3 Synthetic LethalityPMID:24127216
synthetic lethal withcdc12formin Cdc12 Synthetic LethalityPMID:24127216
synthetically rescued bymyo2myosin II heavy chain Synthetic RescuePMID:11056543
enhances phenotype ofmyo2myosin II heavy chain Phenotypic EnhancementPMID:19570908
External References
Database Identifier Description
NBRP SPAC926.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC926.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC926.03 BioGRID Interaction Datasets
Expression Viewer SPAC926.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC926.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC926.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC926.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC926.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC926.03 Transcriptome Viewer (Bähler Lab)
GEO SPAC926.03 GEO profiles
PInt SPAC926.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC926.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC926.03 Fission yeast phenotypic data & analysis
Cyclebase SPAC926.03.1 Cell Cycle Data
SPD / RIKEN05/05F10Orfeome Localization Data
UniProtKB/SwissProtQ9UUG5Myosin regulatory light chain 1
ModBaseQ9UUG5Database of comparative protein structure models
STRINGQ9UUG5Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594364myosin II regulatory light chain Rlc1
RefSeq mRNANM_001019785972h- myosin II regulatory light chain Rlc1 (rlc1), mRNA
European Nucleotide ArchiveCAB54151.1ENA Protein Mapping
UniParcUPI000012F20CUniProt Archive

Literature for rlc1

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016