rlc1 (SPAC926.03)


Gene Standard Namerlc1 Characterisation Statuspublished
Systematic IDSPAC926.03 Feature Typeprotein coding
Synonyms Name Description
Productmyosin II regulatory light chain Rlc1 Product Size184aa, 20.00 kDa
Genomic Location Chromosome I, 3887243-3888497 (1255nt); CDS:3887570-3888124 (555nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
calcium ion binding39
Annotation ExtensionEvidenceWith/FromReference
myosin binding4
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitotic actomyosin contractile ring assembly28
Annotation ExtensionEvidenceWith/FromReference
negative regulation of septation initiation signaling17
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actomyosin contractile ring39
Annotation ExtensionEvidenceWith/FromReference
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
myosin II complex4
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencyrlc1Δ269
normal vegetative cell population growthrlc1Δ819
sensitive to 5-fluorouracilrlc1Δ273
slow vegetative cell population growthrlc1Δ340
viable vegetative cell populationrlc1Δ3831

Cell Phenotype

Term NameGenotypesCount
abnormal actomyosin contractile ring morphologyrlc1Δ6
abolished protein localization to actomyosin contractile ring12
affecting chs2rlc1Δ
abolished protein serine phosphorylation during vegetative growth9
affecting rlc1rlc1-SA (S35A, S36A)
decreased protein localization to actomyosin contractile ring14
affecting shk1rlc1Δ
normal protein localization to cell tip24
affecting shk1rlc1Δ
viable vegetative cell, abnormal cell shape, normal cell sizerlc1Δ215

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
inviable vegetative cell populationrlc1Δ, pxl1Δ
slow vegetative cell population growthrlc1Δ, pxl1Δ

Cell Phenotype

Term NameGenotypes
cutase1Δ, rlc1-SA (S35A, S36A)
inviable mononucleate monoseptate vegetative cellase1Δ, rlc1-SA (S35A, S36A)
septation following abnormal mitotic chromosome segregationase1Δ, rlc1-SA (S35A, S36A)
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in cdc4 myosin II light chain
FYPO affected by mutation in rlc1 myosin II regulatory light chain Rlc1
FYPO affected by mutation in rng2 IQGAP
GO localized by cdc4 myosin II light chain
GO localized by rng2 IQGAP
GO substrate of shk1 PAK-related kinase Shk1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3887243..3888497
5' UTR3887243..3887569PMID:21511999
3' UTR3888125..3888497PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00036 Pfam IPR002048 EF-hand domain 49 77 2
SM00054 SMART IPR002048 EF-hand domain 49 77 9
SM00054 SMART IPR002048 EF-hand domain 118 146 9
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 58 70 12
PS50222 Prosite Profiles IPR002048 EF-hand domain 45 80 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 114 149 20
PTHR23049 HMMPANTHER 16 184 1
PTHR23049:SF34 HMMPANTHER 16 184 1
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 35 108 20
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 116 178 20
SSF47473 SuperFamily IPR011992 EF-hand domain pair 42 177 21

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276

Protein Properties

Ave. residue weight 108.68 Da
Charge -5.00
Codon Adaptation Index 0.43
Isoelectric point 4.70
Molecular weight 20.00 kDa
Number of residues 184
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1675
present during mitotic M phaseS36
present during mitotic M phaseS35
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S35 PMID:21712547
present during mitotic M phase experimental evidence S36 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for rlc1 (SPAC926.03)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8532during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8300during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
9600during GO:0072690PECO:0000012,
PECO:0000005
single cellexperimental evidencePMID:16224022
9297during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2550.36during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
4481.24during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.84during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3422
conserved in vertebrates3397
conserved in eukaryotes only2504
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load gene that interact genetically with SPAC926.03 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withrad2FEN-1 endonuclease Rad2 Positive GeneticPMID:18818364
positive genetic interaction withtas3RITS complex subunit 3 Positive GeneticPMID:22681890
positive genetic interaction withSPAPB1A10.13Schizosaccharomyces specific protein Positive GeneticPMID:22681890
positive genetic interaction withSPCC63.03DNAJ domain protein, DNAJC11 family Positive GeneticPMID:22681890
positive genetic interaction withgim1prefoldin subunit 6, Gim1 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withpnk1DNA kinase/phosphatase Pnk1 Positive GeneticPMID:18818364
positive genetic interaction withtoc1Tor complex Tor2 interacting protein 1 Positive GeneticPMID:22681890
positive genetic interaction withecm313-methyl-2-oxobutanoatehydroxymethyltransferase Ecm31 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsrs2ATP-dependent DNA helicase, UvrD subfamily Positive GeneticPMID:18818364
positive genetic interaction withmrpl1mitochondrial ribosomal protein subunit L1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withcdm1DNA polymerase delta subunit Cdm1 Positive GeneticPMID:22681890
positive genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withpac10prefoldin subunit 3 Pac10 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withSPAC22H10.09Schizosaccharomyces specific protein Positive GeneticPMID:22681890
positive genetic interaction withgpd1glycerol-3-phosphate dehydrogenase Gpd1 Positive GeneticPMID:22681890
positive genetic interaction withlin1U5 snRNP subunit Snu40 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withamt3ammonium transmembrane transporter Amt3 Positive GeneticPMID:22681890
positive genetic interaction withago1argonaute Positive GeneticPMID:22681890
positive genetic interaction withtea2kinesin-like protein Tea2 Positive GeneticPMID:18818364
positive genetic interaction withsnr13-hydroxyisobutyryl-CoA hydrolase snr1 Positive GeneticPMID:22681890
positive genetic interaction withcbp1CENP-B homolog Positive GeneticPMID:18818364
positive genetic interaction witharp6actin-like protein Arp6 Positive GeneticPMID:18818364
positive genetic interaction withcsn1COP9/signalosome complex subunit Csn1 Positive GeneticPMID:18818364
positive genetic interaction withmug178mitochondrial ribosomal protein subunit L51-b (predicted) Positive GeneticPMID:22681890
positive genetic interaction withssu72phosphoric ester hydrolase Ssu72 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withSPBC28E12.04Schizosaccharomyces specific protein Positive GeneticPMID:22681890
negative genetic interaction withSPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:18818364
negative genetic interaction withtif38translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
negative genetic interaction withrad57RecA family ATPase Rad57/Rhp57 Negative GeneticPMID:22681890
negative genetic interaction withyox1MBF complex corepressor Yox1 Negative GeneticPMID:22681890
negative genetic interaction withpuf1pumilio family RNA-binding protein Puf1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpub1HECT-type ubiquitin-protein ligase E3 Pub1 Negative GeneticPMID:22681890
negative genetic interaction withswi3replication fork protection complex subunit Swi3 Negative GeneticPMID:18818364
negative genetic interaction withrad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Negative GeneticPMID:22681890
negative genetic interaction withatp15F0-ATPase epsilon subunit (predicted) Negative GeneticPMID:22681890
negative genetic interaction withppr5mitochondrial PPR repeat protein Ppr5 Negative GeneticPMID:22681890
negative genetic interaction withhrq1RecQ type DNA helicase Hrq1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtos4FHA domain protein Tos4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withask1DASH complex subunit Ask1 Negative GeneticPMID:18818364
negative genetic interaction withSPCC1393.08transcription factor, zf-GATA type (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
negative genetic interaction withgld1mitochondrial glycerol dehydrogenase Gld1 Negative GeneticPMID:22681890
negative genetic interaction withSPBC365.16conserved protein Negative GeneticPMID:22681890
negative genetic interaction withgit5heterotrimeric G protein beta subunit Git5 Negative GeneticPMID:22681890
negative genetic interaction withrad1checkpoint clamp complex protein Rad1 Negative GeneticPMID:22681890
negative genetic interaction withryh1GTPase Ryh1 Negative GeneticPMID:18818364
negative genetic interaction withnud3nuclear distribution protein NUDC homolog Negative GeneticPMID:22681890
negative genetic interaction withrtt109RTT109 family histone lysine acetyltransferase Negative GeneticPMID:22681890
negative genetic interaction withSPAC227.17cconserved eukaryotic protein, DUF3128, human c22orf39 ortholog Negative GeneticPMID:22681890
negative genetic interaction withexg3glucan 1,3-beta-glucosidase Exg3 Negative GeneticPMID:22681890
negative genetic interaction withnup132nucleoporin Nup132 Negative GeneticPMID:22681890
negative genetic interaction withprs5ribose-phosphate pyrophosphokinase Prs5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withuri1unconventional prefoldin chaperone involved protein complex assembly Uri1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmms1E3 ubiquitin ligase complex subunit Mms1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withnrm1MBF complex corepressor Nrm1 Negative GeneticPMID:22681890
negative genetic interaction withscs7sphingosine hydroxylase Scs7 Negative GeneticPMID:22681890
negative genetic interaction withhip1hira protein, histone chaperone Hip1 Negative GeneticPMID:22681890
negative genetic interaction withSPBC1861.05pseudouridine-metabolizing bifunctional protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcgs2cAMP-specific phosphodiesterase Cgs2 Negative GeneticPMID:22681890
negative genetic interaction withgyp51GTPase activating protein Gyp51 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:22681890
negative genetic interaction withgit3G-protein coupled receptor Git3 Negative GeneticPMID:22681890
negative genetic interaction withssb3DNA replication factor A subunit Ssb3 Negative GeneticPMID:22681890
negative genetic interaction withsgf29SAGA complex subunit Sgf29 Negative GeneticPMID:22681890
negative genetic interaction withlag1sphingosine N-acyltransferase Lag1 Negative GeneticPMID:22681890
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:18818364
negative genetic interaction withtco89TORC1 subunit Tco89 Negative GeneticPMID:22681890
negative genetic interaction withSPAC869.03curea transmembrane transporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
negative genetic interaction withSPCC1442.02central kinetochore associated family protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrrg9mitochondrial genome maintenance protein Rrg9 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhmt2sulfide-quinone oxidoreductase Negative GeneticPMID:22681890
negative genetic interaction withamo1nuclear rim protein Amo1 Negative GeneticPMID:18818364
negative genetic interaction withmrc1mediator of replication checkpoint 1 Negative GeneticPMID:18818364
negative genetic interaction withrhn1RNA polymerase II transcription termination factor homolog Negative GeneticPMID:22681890
negative genetic interaction withapm3AP-3 adaptor complex subunit Apm3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
negative genetic interaction withhrp3ATP-dependent DNA helicase Hrp3 Negative GeneticPMID:22681890
negative genetic interaction withmid1medial ring protein Mid1 Negative GeneticPMID:22681890
negative genetic interaction withpop2F-box/WD repeat protein Pop2 Negative GeneticPMID:22681890
negative genetic interaction withcaf1CCR4-Not complex CAF1 family ribonuclease subunit Caf1 Negative GeneticPMID:18818364
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:18818364
negative genetic interaction withrad9checkpoint clamp complex protein Rad9 Negative GeneticPMID:18818364
negative genetic interaction witherg28Erg28 protein (predicted) Negative GeneticPMID:22681890
rescued bycsn5COP9/signalosome complex subunit Csn5 Phenotypic SuppressionPMID:22673520
synthetic lethal withuba3NEDD8 activating enzyme E1-type Uba3 Synthetic LethalityPMID:22673520
External References
Database Identifier Description
NBRP SPAC926.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC926.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC926.03 BioGRID Interaction Datasets
Expression Viewer SPAC926.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC926.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC926.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC926.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC926.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC926.03 Transcriptome Viewer (Bähler Lab)
GEO SPAC926.03 GEO profiles
PInt SPAC926.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC926.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC926.03 Fission yeast phenotypic data & analysis
Cyclebase SPAC926.03.1 Cell Cycle Data
SPD / RIKEN05/05F10Orfeome Localization Data
UniProtKB/SwissProtQ9UUG5Myosin regulatory light chain 1
ModBaseQ9UUG5Database of comparative protein structure models
STRINGQ9UUG5Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594364myosin II regulatory light chain Rlc1
RefSeq mRNANM_001019785972h- myosin II regulatory light chain Rlc1 (rlc1), mRNA
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveCAB54151ENA Protein Mapping
UniParcUPI000012F20CUniProt Archive

Literature for rlc1

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016