rlc1 (SPAC926.03)


Gene Standard Namerlc1 Characterisation Statuspublished
Systematic IDSPAC926.03 Feature Typeprotein coding
Synonyms Name Description
Productmyosin II regulatory light chain Rlc1 Product Size184aa, 20.00 kDa
Genomic Location Chromosome I, 3887243-3888497 (1255nt); CDS:3887570-3888124 (555nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
calcium ion binding39
Annotation ExtensionEvidenceWith/FromReference
myosin binding4
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitotic actomyosin contractile ring assembly28
Annotation ExtensionEvidenceWith/FromReference
negative regulation of septation initiation signaling17
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actomyosin contractile ring38
Annotation ExtensionEvidenceWith/FromReference
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
myosin II complex4
Annotation ExtensionEvidenceWith/FromReference
nucleus2695
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased mating efficiencyrlc1ΔNull252
normal vegetative cell population growthrlc1ΔNull724
sensitive to 5-fluorouracilrlc1ΔNull271
slow vegetative cell population growthrlc1ΔNull326
viable vegetative cell populationrlc1ΔNull3795
unnamed (unrecorded)

Cell Phenotype

Term NameAlleleExpressionCount
abnormal actomyosin contractile ring morphologyrlc1ΔNull4
abolished protein localization to actomyosin contractile ring11
affecting chs2rlc1ΔNull
abolished protein serine phosphorylation during vegetative growth6
affecting rlc1rlc1-SA (S35A, S36A)Not specified
decreased protein localization to actomyosin contractile ring11
affecting shk1rlc1ΔNull
normal protein localization to cell tip21
affecting shk1rlc1ΔNull
viable vegetative cell, abnormal cell shape, normal cell sizerlc1ΔNull215
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in cdc4 myosin II light chain PMID:21376595
FYPO affected by mutation in rlc1 myosin II regulatory light chain Rlc1 PMID:19029336
FYPO affected by mutation in rng2 IQGAP PMID:21376595
GO localized by cdc4 myosin II light chain PMID:21376595
GO localized by rng2 IQGAP PMID:21376595
GO substrate of shk1 PAK-related kinase Shk1 PMID:19029336
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3887243..3888497
mRNA3887243..3888497
5' UTR3887243..3887569PMID:21511999
CDS3887570..3888124
3' UTR3888125..3888497PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13405 Pfam IPR002048 EF-hand domain 119 147 4
PF00036 Pfam IPR002048 EF-hand domain 49 77 2
SM00054 SMART IPR002048 EF-hand domain 49 77 9
SM00054 SMART IPR002048 EF-hand domain 118 146 9
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 58 70 12
PS50222 Prosite Profiles IPR002048 EF-hand domain 45 80 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 114 149 20
PTHR23049 HMMPANTHER 7 177 1
PTHR23049:SF34 HMMPANTHER 7 177 1
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 35 108 20
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 116 178 20
SSF47473 SuperFamily 42 177 21

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276

Protein Properties

Ave. residue weight 108.68 Da
Charge -5.00
Isoelectric point 4.70
Molecular weight 20.00 kDa
Number of residues 184
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS35
present during mitotic M phaseS36
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S35 PMID:21712547
present during mitotic M phase experimental evidence S36 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8532during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
8300during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
9297during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
9600during GO:0072690PECO:0000005,
PECO:0000012
single cellexperimental evidencePMID:16224022
2550.36during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
4481.24during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.84during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturescdc4myosin II light chain Affinity Capture-WesternPMID:11069761
affinity capturescdc4myosin II light chain Affinity Capture-WesternPMID:11056543
affinity captured bymyo2myosin II heavy chain Affinity Capture-WesternPMID:15504913
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:11069761
affinity captured bymyo2myosin II heavy chain Affinity Capture-WesternPMID:11069761
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:11056543
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:19570908
co-purifies withmyo2myosin II heavy chain Co-purificationPMID:19570908
affinity capturesmyp2myosin II heavy chain Myo3 Affinity Capture-WesternPMID:11069761
affinity captured bymyp2myosin II heavy chain Myo3 Affinity Capture-WesternPMID:11069761
affinity captured bypxl1paxillin-like protein Pxl1 Affinity Capture-WesternPMID:18256290
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withcdc12formin Cdc12 Synthetic LethalityPMID:24127216
synthetic growth defect withcdc12formin Cdc12 Synthetic Growth DefectPMID:24115772
synthetic growth defect withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic Growth DefectPMID:19139265
synthetic lethal withfor3formin For3 Synthetic LethalityPMID:24127216
synthetic growth defect withimp2contractile ring protein Imp2 Synthetic Growth DefectPMID:19139265
synthetically rescued bymyo2myosin II heavy chain Synthetic RescuePMID:11056543
enhances phenotype ofmyo2myosin II heavy chain Phenotypic EnhancementPMID:19570908
synthetic growth defect withmyo51myosin type V Synthetic Growth DefectPMID:24798735
synthetic growth defect withpxl1paxillin-like protein Pxl1 Synthetic Growth DefectPMID:18256290
synthetic growth defect withrng8contractile ring myosin V regulator Rng8 Synthetic Growth DefectPMID:24798735
synthetic growth defect withrng9contractile ring myosin V regulator Rng9 Synthetic Growth DefectPMID:24798735
External References
Database Identifier Description
NBRP SPAC926.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC926.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC926.03 BioGRID Interaction Datasets
Expression Viewer SPAC926.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC926.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC926.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC926.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC926.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC926.03 Transcriptome Viewer (Bähler Lab)
GEO SPAC926.03 GEO profiles
PInt SPAC926.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC926.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC926.03 Fission yeast phenotypic data & analysis
Cyclebase SPAC926.03.1 Cell Cycle Data
SPD / RIKEN05/05F10Orfeome Localization Data
UniProtKB/SwissProtQ9UUG5Myosin regulatory light chain 1
ModBaseQ9UUG5Database of comparative protein structure models
STRINGQ9UUG5Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594364myosin II regulatory light chain Rlc1
RefSeq mRNANM_001019785972h- myosin II regulatory light chain Rlc1 (rlc1), mRNA
European Nucleotide ArchiveCAB54151.1ENA Protein Mapping
UniParcUPI000012F20CUniProt Archive

Literature for rlc1

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015