rlc1 (SPAC926.03)


Gene Standard Namerlc1 Characterisation Statuspublished
Systematic IDSPAC926.03 Feature Typeprotein coding
Synonyms Name Description
Productmyosin II regulatory light chain Rlc1 Product Size184aa, 20.00 kDa
Genomic Location Chromosome I, 3887243-3888497 (1255nt); CDS:3887570-3888124 (555nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005509calcium ion bindingIEAIPR002048GO_REF:000000252
GO:0017022myosin bindingIPImyp2PMID:110697614
IPImyo2PMID:11056543
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000915actomyosin contractile ring assemblyIMPPMID:1106976126
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005826actomyosin contractile ringIDAPMID:1105654331
GO:0005829cytosolIDAPMID:168233722316
GO:0016460myosin II complexNASGO_REF:00000014
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyrlc1ΔNullPMID:204732893760
Microscopyrlc1ΔNullPECO:0000005, PECO:0000137PMID:23697806
not recorded (unrecorded)PMID:11069761

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002561abolished protein localization to actomyosin contractile ring10
penetrance FYPO_EXT:0000001
affecting chs2
Microscopyrlc1ΔNullPECO:0000006PMID:16772338
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidencerlc1ΔNullPECO:0000015PMID:23950735230
FYPO:0001510viable vegetative cell, abnormal cell shape, normal cell size215
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000001
Microscopyrlc1ΔNullPECO:0000137, PECO:0000005PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in cdc4 myosin II light chain PMID:21376595
FYPO affected by mutation in gef2 RhoGEF Gef2 PMID:23349808
FYPO affected by mutation in pxl1 paxillin-like protein Pxl1 PMID:18256290
FYPO affected by mutation in rng2 IQGAP PMID:21376595
GO localized by cdc4 myosin II light chain PMID:21376595
GO localized by rng2 IQGAP PMID:21376595
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
138872433888497

UTRs

Region Coordinates Reference
five_prime_UTR3887243..3887569PMID:21511999
three_prime_UTR3888125..3888497PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00036 Pfam IPR002048 EF-hand domain 49 77 6
PF13405 Pfam IPR002048 EF-hand domain 119 147 6
SM00054 SMART IPR002048 EF-hand domain 49 77 14
SM00054 SMART IPR002048 EF-hand domain 118 146 14
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 58 70 12
PS50222 Prosite Profiles IPR002048 EF-hand domain 45 80 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 114 149 20
PTHR23049:SF34 HMMPANTHER 7 177 1
PTHR23049 HMMPANTHER 7 177 1
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 35 108 20
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 116 178 20
SSF47473 SuperFamily 42 177 21

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276

Protein Properties

Ave. residue weight 108.68 Da
Charge -5.00
Isoelectric point 4.70
Molecular weight 20.00 kDa
Number of residues 184
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS36PMID:217125471663
present during mitotic M phase
present during mitotic M phaseS35PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
9600during GO:0072690PECO:0000012,
PECO:0000005
experimental evidencePMID:16224022
4481.24during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
2550.36during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
9297during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
8300during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
8532during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
0.84during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
rng9contractile ring myosin V regulator Rng9 Synthetic Growth DefectPMID:24798735
myo2myosin II heavy chain Synthetic RescuePMID:11056543
Phenotypic EnhancementPMID:19570908
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic Growth DefectPMID:19139265
rng8contractile ring myosin V regulator Rng8 Synthetic Growth DefectPMID:24798735
myo51myosin type V Synthetic Growth DefectPMID:24798735
for3formin For3 Synthetic LethalityPMID:24127216
imp2contractile ring protein Imp2 Synthetic Growth DefectPMID:19139265
cdc12formin Cdc12 Synthetic LethalityPMID:24127216
Synthetic Growth DefectPMID:24115772
pxl1paxillin-like protein Pxl1 Synthetic Growth DefectPMID:18256290
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
pxl1paxillin-like protein Pxl1 Affinity Capture-WesternPMID:18256290
myo2myosin II heavy chain Affinity Capture-WesternPMID:15504913
Affinity Capture-WesternPMID:11069761
Affinity Capture-WesternPMID:11056543
Co-purificationPMID:19570908
Affinity Capture-Western
cdc4myosin II light chain Affinity Capture-WesternPMID:11069761
Affinity Capture-WesternPMID:11056543
myp2myosin II heavy chain Myo3 Affinity Capture-WesternPMID:11069761
External References
Database Identifier Description
NBRP SPAC926.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC926.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC926.03 BioGRID Interaction Datasets
Expression Viewer SPAC926.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC926.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC926.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC926.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC926.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC926.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC926.03 Cell Cycle Data
GEO SPAC926.03 GEO profiles
PInt SPAC926.03 Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2543545myosin II regulatory light chain Rlc1
EntrezGene2543545myosin II regulatory light chain Rlc1
SPD / RIKEN05/05F10Orfeome Localization Data
UniProtKB/SwissProtQ9UUG5Myosin regulatory light chain 1
ModBaseQ9UUG5Database of comparative protein structure models
STRINGQ9UUG5Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594364myosin II regulatory light chain Rlc1
RefSeq mRNANM_001019785972h- myosin II regulatory light chain Rlc1 (rlc1), mRNA
European Nucleotide ArchiveCAB54151ENA Protein Mapping
European Nucleotide ArchiveCAB54151.1ENA Protein Mapping
UniParcUPI000012F20CUniProt Archive

Literature for rlc1

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014