hsp90 (SPAC926.04c)


Gene Standard Namehsp90 Characterisation Statuspublished
Systematic IDSPAC926.04c Feature Typeprotein coding
Synonymsgit10, swo1 Name DescriptionGlucose Insensitive Transcription
ProductHsp90 chaperone Product Size704aa, 80.60 kDa
Genomic Location Chromosome I, 3891157-3888558 (2600nt); CDS:3890888-3888774 (2115nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding560
Annotation ExtensionEvidenceWith/FromReference
ATPase activity239
Annotation ExtensionEvidenceWith/FromReference
protein binding872
Annotation ExtensionEvidenceWith/FromReference
unfolded protein binding42
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

protein folding


Term NameCount
'de novo' protein folding6
Annotation ExtensionEvidenceWith/FromReference
chaperone-mediated protein folding10
Annotation ExtensionEvidenceWith/FromReference
protein folding100
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4212
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
mitochondrion752
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
abolished cell population growth at high temperatureswo1-26 (G84C)37
decreased cell population growth at high temperaturegit10-201 (L338P)173
increased mating efficiencygit10-201 (L338P)36
inviable vegetative cell populationswo1-26 (G84C)1452
hsp90Δ
loss of viability at high temperatureswo1-26 (G84C)56
swo1-21 (L654R)
normal vegetative cell population growthgit10-201 (L338P)851
swo1-26 (G84C)

Cell Phenotype

Term NameGenotypesCount
abnormal negative regulation of transcription by glucose9
affecting fbp1git10-201 (L338P)
abnormal nucleus localization during vegetative growthswo1-26 (G84C)44
swo1-21 (L654R)
decreased negative regulation of transcription by glucosegit10-201 (L338P)4
increased level of glucose starvation gene mRNA during vegetative growthgit10-201 (L338P)1
inviable spheroid vegetative cellswo1-26 (G84C)60
inviable sporehsp90Δ476
inviable swollen vegetative cellswo1-26 (G84C)21
vegetative cell lysisswo1-26 (G84C)93
swo1-21 (L654R)
viable small vegetative cellswo1-26 (G84C)51

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased vegetative cell population growthswo1-26 (G84C), wee1+ (wild type)
swo1-26 (G84C), wee-115

Cell Phenotype

Term NameGenotypes
decreased protein level during vegetative growth
affecting wee1swo1-26 (G84C), wee-115
normal negative regulation of transcription by glucose
affecting fbp1git10-201 (L338P), cyr1+
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in lkh1 dual specificity protein kinase Lkh1
FYPO affected by mutation in mas5 DNAJ domain protein Mas5 (predicted)
FYPO affected by mutation in ssa2 heat shock protein Ssa2
GO regulated by aha1 chaperone activator Aha1 (predicted)
GO regulated by sti1 chaperone activator Sti1 (predicted)
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3891157..3888558
mRNA3891157..3888558
5' UTR3891157..3890889PMID:21511999
CDS3890888..3888774
3' UTR3888773..3888558PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02518 Pfam IPR003594 Histidine kinase-like ATPase, C-terminal domain 27 180 6
PF00183 Pfam IPR001404 Heat shock protein Hsp90 family 185 699 1
SM00387 SMART IPR003594 Histidine kinase-like ATPase, C-terminal domain 27 165 5
PS00298 Prosite Patterns IPR019805 Heat shock protein Hsp90, conserved site 25 34 1
PTHR11528 HMMPANTHER IPR001404 Heat shock protein Hsp90 family 1 704 1
3.30.565.10 Gene3D IPR003594 Histidine kinase-like ATPase, C-terminal domain 4 212 8
0043579 SuperFamily 546 668 1
SSF55874 SuperFamily IPR003594 Histidine kinase-like ATPase, C-terminal domain 4 211 8
SSF54211 SuperFamily IPR020568 Ribosomal protein S5 domain 2-type fold 267 522 29
PIRSF002583 PIRSF IPR001404 Heat shock protein Hsp90 family 1 704 1
Coil ncoils Predicted coiled-coil protein (DUF2205) 529 556 1057
Coil ncoils Predicted coiled-coil protein (DUF2205) 238 268 1057
Coil ncoils Predicted coiled-coil protein (DUF2205) 682 702 1057
MF_00505 HAMAP IPR001404 Heat shock protein Hsp90 family 2 669 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 93 110 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 5 25 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 170 187 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 118 140 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 75 92 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 188 206 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 26 48 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.48 Da
Charge -32.50
Codon Adaptation Index 0.81
Isoelectric point 4.60
Molecular weight 80.60 kDa
Number of residues 704
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1677
present during mitotic M phaseS290
present during cellular response to thiabendazole, mitotic M phaseS277
present during cellular response to thiabendazole, mitotic M phaseS245
present during mitotic M phaseS451
present during cellular response to thiabendazoleS366
present during cellular response to thiabendazole, mitotic M phaseS374
S245, S277, S290, S374, S451
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S290 PMID:21712547
present during cellular response to thiabendazole IDA S277 PMID:18257517
present during cellular response to thiabendazole IDA S245 PMID:18257517
present during mitotic M phase experimental evidence S245 PMID:21712547
present during mitotic M phase experimental evidence S451 PMID:21712547
present during cellular response to thiabendazole IDA S366 PMID:18257517
present during mitotic M phase experimental evidence S374 PMID:21712547
present during mitotic M phase experimental evidence S277 PMID:21712547
present during cellular response to thiabendazole IDA S374 PMID:18257517
experimental evidence S277 PMID:24763107
experimental evidence S290 PMID:24763107
experimental evidence S245 PMID:24763107
experimental evidence S451 PMID:24763107
experimental evidence S374 PMID:24763107
O-phospho-L-threonine 701
present during mitotic M phaseT280
present during mitotic M phaseT224
present during mitotic M phaseT268
present during mitotic M phaseT292
T68
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T280 PMID:21712547
present during mitotic M phase experimental evidence T224 PMID:21712547
present during mitotic M phase experimental evidence T268 PMID:21712547
present during mitotic M phase experimental evidence T292 PMID:21712547
experimental evidence T68 PMID:24763107
phosphorylated residue 1932
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysineK99, K418, K436, K560 512
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence K436 PMID:26412298
mass spectrometry evidence K418 PMID:26412298
mass spectrometry evidence K99 PMID:26412298
IDA PMID:26412298
mass spectrometry evidence K560 PMID:26412298
sumoylated lysine 173
Annotation ExtensionEvidenceResidueReference
IDA PMID:26404184
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelincreased during GO:0034605Western blot evidencePMID:7813446

Quantitative Gene Expression

View graphical display of gene expression data for hsp90 (SPAC926.04c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
879705during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
883920during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
886374during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
940434during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
284621during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
817914during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
60584.66during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
160during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
13during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancercancer_census148
Subunit Composition
DescriptionQualifierReferenceCount
homomeric(2) PMID:2366492753
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
chaperone12
Pathway
DescriptionQualifierReferenceCount
PKA PMID:184309262
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in bacteria1002
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC926.04c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bywee1M phase inhibitor protein kinase Wee1 Affinity Capture-WesternPMID:7813446
affinity captured bydcp2mRNA decapping complex catalytic subunit Dcp2 Affinity Capture-MSPMID:23319050
affinity captured bylsd1histone demethylase SWIRM1 Affinity Capture-MSPMID:16990277
affinity captured byslm9hira protein Slm9 Affinity Capture-MSPMID:20976105
affinity captured bycdc37Hsp90 co-chaperone Cdc37 Affinity Capture-WesternPMID:16098195
affinity captured bysog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
affinity captured bymik1mitotic inhibitor kinase Mik1 Affinity Capture-WesternPMID:11298745
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
affinity captured bycid13poly(A) polymerase Cid13 Affinity Capture-MSPMID:12062100
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:15755919
affinity capturescdc2cyclin-dependent protein kinase Cdk1/Cdc2 Affinity Capture-WesternPMID:22543982
affinity capturesplo1Polo kinase Plo1 Affinity Capture-WesternPMID:22543982
co-fractionates withcdc37Hsp90 co-chaperone Cdc37 Co-fractionationPMID:16098195
forms complex withaha1chaperone activator Aha1 (predicted) Reconstituted ComplexPMID:23664927
forms complex withhsp90Hsp90 chaperone Reconstituted ComplexPMID:23664927
forms complex withsti1chaperone activator Sti1 (predicted) Reconstituted ComplexPMID:23664927
binds DNA-binding domain construct withrng3UCS-domain protein Rng3 Two-hybridPMID:15755919
binds DNA-binding domain construct withmyo2myosin II heavy chain Two-hybridPMID:15755919
binds activation domain construct withaha1chaperone activator Aha1 (predicted) Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC926.04c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetically rescueswee1M phase inhibitor protein kinase Wee1 Synthetic RescuePMID:7813446
rescued by overexpression ofsck1serine/threonine protein kinase Sck1 Dosage RescuePMID:7498728
rescued by overexpression ofcyr1adenylate cyclase Dosage RescuePMID:1849107
rescued by overexpression ofptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:8196617
synthetic growth defect withplo1Polo kinase Plo1 Synthetic Growth DefectPMID:22543982
synthetic growth defect withcdc7serine/threonine protein kinase Cdc7 Synthetic Growth DefectPMID:22543982
synthetic growth defect withsid2NDR kinase Sid2 Synthetic Growth DefectPMID:22543982
overexpression rescuescdc37Hsp90 co-chaperone Cdc37 Dosage RescuePMID:16098195
overexpression rescuescdc14SIN component Cdc14 Dosage RescuePMID:11791728
synthetic lethal withrng3UCS-domain protein Rng3 Synthetic LethalityPMID:15755919
synthetic lethal withmyo2myosin II heavy chain Synthetic LethalityPMID:15755919
phenotype enhanced bycdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic EnhancementPMID:22543982
rescued bypyp1tyrosine phosphatase Pyp1 Phenotypic SuppressionPMID:8832414
enhances phenotype ofcdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic EnhancementPMID:10102358
External References
Database Identifier Description
NBRP SPAC926.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC926.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC926.04c BioGRID Interaction Datasets
Expression Viewer SPAC926.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC926.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC926.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC926.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC926.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC926.04c Transcriptome Viewer (Bähler Lab)
GEO SPAC926.04c GEO profiles
PInt SPAC926.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC926.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC926.04c Fission yeast phenotypic data & analysis
Cyclebase SPAC926.04c.1 Cell Cycle Data
SPD / RIKEN28/28A02Orfeome Localization Data
UniProtKB/SwissProtP41887Heat shock protein 90 homolog
ModBaseP41887Database of comparative protein structure models
STRINGP41887Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594365Hsp90 chaperone
RefSeq mRNANM_001019786972h- Hsp90 chaperone (hsp90), mRNA
European Nucleotide ArchiveAAC41646.1ENA Protein Mapping
European Nucleotide ArchiveCAB54152.1ENA Protein Mapping
UniParcUPI000012CCBEUniProt Archive

Literature for hsp90

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016