hsp90 (SPAC926.04c)


Gene Standard Namehsp90 Characterisation Statuspublished
Systematic IDSPAC926.04c Feature Typeprotein coding
Synonymsgit10, swo1 Name DescriptionGlucose Insensitive Transcription
ProductHsp90 chaperone Product Size704aa, 80.60 kDa
Genomic Location Chromosome I, 3891157-3888558 (2600nt); CDS:3890888-3888774 (2115nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0042623ATPase activity, coupledISOSGD:S000006161GO_REF:0000024171
GO:0044183protein binding involved in protein foldingEXPPMID:184309262
GO:0051082unfolded protein bindingIEAIPR001404GO_REF:000000242
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006458'de novo' protein foldingISOSGD:S000006161GO_REF:00000247
GO:0034605cellular response to heatISOSGD:S000004798GO_REF:000002436
GO:0061077chaperone-mediated protein foldingNASGO_REF:000000111
GO:0006457protein foldingIMPPMID:11791728111
EXPPMID:18430926
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISOSGD:S000004798GO_REF:00000244201
GO:0005829cytosolIDAPMID:168233722316
GO:0005739mitochondrionISOSGD:S000004798GO_REF:0000024758
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyhsp90ΔNullPMID:204732891315
Microscopyhsp90ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0001357normal vegetative cell population growthCell growth assaygit10-201 (L338P)Not specifiedPECO:0000005PMID:18430926466
Cell growth assaygit10-201 (L338P)Not specifiedPECO:0000004PMID:18430926

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000031abnormal conjugationMicroscopygit10-201 (L338P)Not specifiedPMID:1843092669
FYPO:0000044abnormal negative regulation of transcription by glucose9
affecting fbp1Transcript expression level evidencegit10-201 (L338P)Not specifiedPECO:0000162PMID:1849107
affecting fbp1Transcript expression level evidencegit10-201 (L338P)Not specifiedPECO:0000162, PECO:0000056PMID:1849107
Reporter gene assaygit10-201 (L338P)Not specifiedPMID:18430926
FYPO:0001338abnormal nucleus localization during vegetative growthMicroscopyswo1-26 (G84C)Not specifiedPECO:0000004, PECO:0000126PMID:184309263
Microscopyswo1-21 (L654R)Not specifiedPECO:0000004, PECO:0000126PMID:18430926
FYPO:0001801inviable cell at high temperatureCell growth assayswo1-21 (L654R)Not specifiedPMID:1843092623
Cell growth assayswo1-26 (G84C)Not specifiedPMID:18430926
FYPO:0002151inviable spore457
penetrance FYPO_EXT:0000001Microscopyhsp90ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0000023small cellnot recorded (unrecorded)PMID:781344667
FYPO:0000647vegetative cell lysisMicroscopyswo1-21 (L654R)Not specifiedPECO:0000004, PECO:0000126PMID:1843092643
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in lkh1 dual specificity protein kinase Lkh1 PMID:23956636
FYPO affected by mutation in mas5 DNAJ domain protein Mas5 (predicted) PMID:24146635
FYPO affected by mutation in ssa1 heat shock protein Ssa1 (predicted) PMID:24146635
FYPO affected by mutation in ssa2 heat shock protein Ssa2 PMID:24146635
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
138911573888558

UTRs

Region Coordinates Reference
five_prime_UTR3891157..3890889PMID:21511999
three_prime_UTR3888773..3888558PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02518 Pfam IPR003594 Histidine kinase-like ATPase, ATP-binding domain 29 161 6
PF00183 Pfam IPR001404 Heat shock protein Hsp90 family 184 704 1
SM00387 SMART IPR003594 Histidine kinase-like ATPase, ATP-binding domain 27 165 8
PS00298 Prosite Patterns IPR019805 Heat shock protein Hsp90, conserved site 25 34 1
PTHR11528 HMMPANTHER IPR001404 Heat shock protein Hsp90 family 4 704 1
3.30.565.10 Gene3D IPR003594 Histidine kinase-like ATPase, ATP-binding domain 4 212 8
SSF54211 SuperFamily IPR020568 Ribosomal protein S5 domain 2-type fold 267 522 29
SSF55874 SuperFamily IPR003594 Histidine kinase-like ATPase, ATP-binding domain 4 211 8
0043579 SuperFamily 546 668 1
PIRSF002583 PIRSF IPR001404 Heat shock protein Hsp90 family 3 696 1
Coil ncoils Rabaptin coiled-coil domain 529 557 968
Coil ncoils Rabaptin coiled-coil domain 682 703 968
Coil ncoils Rabaptin coiled-coil domain 238 269 968
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 75 92 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 188 206 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 26 48 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 5 25 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 93 110 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 118 140 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 170 187 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.48 Da
Charge -32.50
Isoelectric point 4.60
Molecular weight 80.60 kDa
Number of residues 704
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS451PMID:247631071663
present during cellular response to thiabendazoleIDAS374PMID:18257517
S245PMID:24763107
present during mitotic M phaseS374PMID:21712547
S277PMID:24763107
S374PMID:24763107
present during mitotic M phaseS290PMID:21712547
present during cellular response to thiabendazoleIDAS245PMID:18257517
present during mitotic M phaseS245PMID:21712547
present during mitotic M phaseS451PMID:21712547
present during mitotic M phaseS277PMID:21712547
present during cellular response to thiabendazoleIDAS366PMID:18257517
present during cellular response to thiabendazoleIDAS277PMID:18257517
S290PMID:24763107
MOD:00047O-phospho-L-threonineT292PMID:21712547682
present during mitotic M phase
present during mitotic M phaseT224PMID:21712547
present during mitotic M phaseT268PMID:21712547
present during mitotic M phaseT280PMID:21712547
T68PMID:24763107
MOD:00696phosphorylated residueNASPMID:182575171915
NASPMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
940434during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
879705during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
886374during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
284621during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
60584.66during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
883920during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
817914during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
13during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
160during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancercancer_census141
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
chaperone12
Pathway
DescriptionQualifierReferenceCount
PKA PMID:184309262
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3393
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:8196617
myo2myosin II heavy chain Synthetic LethalityPMID:15755919
sck1serine/threonine protein kinase Sck1 Dosage RescuePMID:7498728
cyr1adenylate cyclase Dosage RescuePMID:1849107
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic EnhancementPMID:22543982
Phenotypic EnhancementPMID:10102358
sid2NDR kinase Sid2 Synthetic Growth DefectPMID:22543982
pyp1tyrosine phosphatase Pyp1 Phenotypic SuppressionPMID:8832414
plo1Polo kinase Plo1 Synthetic Growth DefectPMID:22543982
rng3UCS-domain protein Rng3 Synthetic LethalityPMID:15755919
cdc14SIN component Cdc14 Dosage RescuePMID:11791728
cdc37Hsp90 co-chaperone Cdc37 Dosage RescuePMID:16098195
cdc7serine/threonine protein kinase Cdc7 Synthetic Growth DefectPMID:22543982
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
dcp2mRNA decapping complex catalytic subunit Dcp2 Affinity Capture-MSPMID:23319050
cid13poly(A) polymerase Cid13 Affinity Capture-MSPMID:12062100
myo2myosin II heavy chain Two-hybridPMID:15755919
Affinity Capture-Western
SPBC1711.08chaperone activator Aha1 (predicted) Reconstituted ComplexPMID:23664927
hsp90Hsp90 chaperone Reconstituted ComplexPMID:23664927
cdc37Hsp90 co-chaperone Cdc37 Co-fractionationPMID:16098195
Affinity Capture-Western
mik1mitotic inhibitor kinase Mik1 Affinity Capture-WesternPMID:11298745
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Affinity Capture-WesternPMID:22543982
sti1chaperone activator Sti1 (predicted) Reconstituted ComplexPMID:23664927
lsd1histone demethylase SWIRM1 Affinity Capture-MSPMID:16990277
plo1Polo kinase Plo1 Affinity Capture-WesternPMID:22543982
rng3UCS-domain protein Rng3 Two-hybridPMID:15755919
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
sog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
slm9hira protein Slm9 Affinity Capture-MSPMID:20976105
wee1M phase inhibitor protein kinase Wee1 Affinity Capture-WesternPMID:11298745
External References
Database Identifier Description
NBRP SPAC926.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC926.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC926.04c BioGRID Interaction Datasets
Expression Viewer SPAC926.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC926.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC926.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC926.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC926.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC926.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC926.04c Cell Cycle Data
GEO SPAC926.04c GEO profiles
PInt SPAC926.04c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2543539Hsp90 chaperone
EntrezGene2543539Hsp90 chaperone
SPD / RIKEN28/28A02Orfeome Localization Data
UniProtKB/SwissProtP41887Heat shock protein 90 homolog
ModBaseP41887Database of comparative protein structure models
STRINGP41887Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594365Hsp90 chaperone
RefSeq mRNANM_001019786972h- Hsp90 chaperone (hsp90), mRNA
European Nucleotide ArchiveAAC41646ENA Protein Mapping
European Nucleotide ArchiveAAC41646.1ENA Protein Mapping
European Nucleotide ArchiveCAB54152ENA Protein Mapping
European Nucleotide ArchiveCAB54152.1ENA Protein Mapping
UniParcUPI000012CCBEUniProt Archive

Literature for hsp90

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014