hsp90 (SPAC926.04c)


Gene Standard Namehsp90 Characterisation Statuspublished
Systematic IDSPAC926.04c Feature Typeprotein coding
Synonymsgit10, swo1 Name DescriptionGlucose Insensitive Transcription
ProductHsp90 chaperone Product Size704aa, 80.60 kDa
Genomic Location Chromosome I, 3891157-3888558 (2600nt); CDS:3890888-3888774 (2115nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
ATPase activity236
Annotation ExtensionEvidenceWith/FromReference
protein binding856
Annotation ExtensionEvidenceWith/FromReference
protein binding involved in protein folding1
Annotation ExtensionEvidenceWith/FromReference
unfolded protein binding41
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

protein folding


Term NameCount
'de novo' protein folding6
Annotation ExtensionEvidenceWith/FromReference
chaperone-mediated protein folding10
Annotation ExtensionEvidenceWith/FromReference
protein folding103
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4207
Annotation ExtensionEvidenceWith/FromReference
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
mitochondrion761
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationhsp90ΔNull1435
swo1-26 (G84C)Not specified
normal vegetative cell population growthgit10-201 (L338P)Not specified724
swo1-26 (G84C)Not specified

Cell Phenotype

Term NameAlleleExpressionCount
abnormal conjugationgit10-201 (L338P)Not specified65
abnormal negative regulation of transcription by glucosegit10-201 (L338P)Not specified9
affecting fbp1git10-201 (L338P)Not specified
abnormal nucleus localization during vegetative growthswo1-21 (L654R)Not specified13
swo1-26 (G84C)Not specified
inviable cell at high temperatureswo1-21 (L654R)Not specified22
swo1-26 (G84C)Not specified
inviable spheroid vegetative cellswo1-26 (G84C)Not specified60
inviable sporehsp90ΔNull470
inviable swollen vegetative cellswo1-26 (G84C)Not specified32
vegetative cell lysisswo1-21 (L654R)Not specified83
swo1-26 (G84C)Not specified
viable small vegetative cellswo1-26 (G84C)Not specified43
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in lkh1 dual specificity protein kinase Lkh1 PMID:23956636
FYPO affected by mutation in mas5 DNAJ domain protein Mas5 (predicted) PMID:24146635
FYPO affected by mutation in ssa2 heat shock protein Ssa2 PMID:24146635
GO regulated by aha1 chaperone activator Aha1 (predicted) PMID:23664927
GO regulated by sti1 chaperone activator Sti1 (predicted) PMID:23664927
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3891157..3888558
mRNA3891157..3888558
5' UTR3891157..3890889PMID:21511999
CDS3890888..3888774
3' UTR3888773..3888558PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00183 Pfam IPR001404 Heat shock protein Hsp90 family 184 704 1
PF02518 Pfam IPR003594 Histidine kinase-like ATPase, C-terminal domain 29 161 6
SM00387 SMART IPR003594 Histidine kinase-like ATPase, C-terminal domain 27 165 5
PS00298 Prosite Patterns IPR019805 Heat shock protein Hsp90, conserved site 25 34 1
PTHR11528 HMMPANTHER IPR001404 Heat shock protein Hsp90 family 4 704 1
3.30.565.10 Gene3D IPR003594 Histidine kinase-like ATPase, C-terminal domain 4 212 8
SSF54211 SuperFamily IPR020568 Ribosomal protein S5 domain 2-type fold 267 522 29
SSF55874 SuperFamily IPR003594 Histidine kinase-like ATPase, C-terminal domain 4 211 8
0043579 SuperFamily 546 668 1
PIRSF002583 PIRSF IPR001404 Heat shock protein Hsp90 family 3 696 1
Coil ncoils Rabaptin coiled-coil domain 238 269 968
Coil ncoils Rabaptin coiled-coil domain 682 703 968
Coil ncoils Rabaptin coiled-coil domain 529 557 968
MF_00505 hamap IPR001404 Heat shock protein Hsp90 family 2 669 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 170 187 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 26 48 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 118 140 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 93 110 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 75 92 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 5 25 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 188 206 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.48 Da
Charge -32.50
Isoelectric point 4.60
Molecular weight 80.60 kDa
Number of residues 704
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS245, S277, S290, S374, S451 1672
present during cellular response to thiabendazoleS366
present during mitotic M phaseS290
present during cellular response to thiabendazole, mitotic M phaseS374
present during mitotic M phaseS451
present during cellular response to thiabendazole, mitotic M phaseS277
present during mitotic M phase, cellular response to thiabendazoleS245
Annotation ExtensionEvidenceResidueReference
experimental evidence S245 PMID:24763107
present during cellular response to thiabendazole IDA S245 PMID:18257517
present during mitotic M phase experimental evidence S245 PMID:21712547
experimental evidence S277 PMID:24763107
present during mitotic M phase experimental evidence S277 PMID:21712547
present during cellular response to thiabendazole IDA S277 PMID:18257517
experimental evidence S290 PMID:24763107
present during mitotic M phase experimental evidence S290 PMID:21712547
present during cellular response to thiabendazole IDA S366 PMID:18257517
experimental evidence S374 PMID:24763107
present during cellular response to thiabendazole IDA S374 PMID:18257517
present during mitotic M phase experimental evidence S374 PMID:21712547
experimental evidence S451 PMID:24763107
present during mitotic M phase experimental evidence S451 PMID:21712547
O-phospho-L-threonine 698
present during mitotic M phaseT224
present during mitotic M phaseT280
present during mitotic M phaseT292
T68
present during mitotic M phaseT268
Annotation ExtensionEvidenceResidueReference
experimental evidence T68 PMID:24763107
present during mitotic M phase experimental evidence T224 PMID:21712547
present during mitotic M phase experimental evidence T268 PMID:21712547
present during mitotic M phase experimental evidence T280 PMID:21712547
present during mitotic M phase experimental evidence T292 PMID:21712547
phosphorylated residue 1926
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelincreased during GO:0034605Western blot evidencePMID:7813446

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
879705during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
883920during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
886374during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
940434during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
817914during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
284621during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
60584.66during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
160during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
13during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancercancer_census142
Subunit Composition
DescriptionQualifierReferenceCount
homomeric(2) PMID:2366492749
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
chaperone12
Pathway
DescriptionQualifierReferenceCount
PKA PMID:184309262
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
conserved in metazoa3430
conserved in vertebrates3405
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
forms complex withaha1chaperone activator Aha1 (predicted) Reconstituted ComplexPMID:23664927
affinity capturescdc2cyclin-dependent protein kinase Cdk1/Cdc2 Affinity Capture-WesternPMID:22543982
affinity captured bycdc37Hsp90 co-chaperone Cdc37 Affinity Capture-WesternPMID:16098195
co-fractionates withcdc37Hsp90 co-chaperone Cdc37 Co-fractionationPMID:16098195
affinity captured bycid13poly(A) polymerase Cid13 Affinity Capture-MSPMID:12062100
affinity captured bydcp2mRNA decapping complex catalytic subunit Dcp2 Affinity Capture-MSPMID:23319050
forms complex withhsp90Hsp90 chaperone Reconstituted ComplexPMID:23664927
affinity captured bylsd1histone demethylase SWIRM1 Affinity Capture-MSPMID:16990277
affinity captured bymik1mitotic inhibitor kinase Mik1 Affinity Capture-WesternPMID:11298745
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:15755919
binds DNA-binding domain construct withmyo2myosin II heavy chain Two-hybridPMID:15755919
affinity capturesplo1Polo kinase Plo1 Affinity Capture-WesternPMID:22543982
binds DNA-binding domain construct withrng3UCS-domain protein Rng3 Two-hybridPMID:15755919
affinity captured byslm9hira protein Slm9 Affinity Capture-MSPMID:20976105
affinity captured bysog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
forms complex withsti1chaperone activator Sti1 (predicted) Reconstituted ComplexPMID:23664927
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
affinity captureswee1M phase inhibitor protein kinase Wee1 Affinity Capture-WesternPMID:7813446
affinity captured bywee1M phase inhibitor protein kinase Wee1 Affinity Capture-WesternPMID:11298745
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
overexpression rescuescdc14SIN component Cdc14 Dosage RescuePMID:11791728
phenotype enhanced bycdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic EnhancementPMID:22543982
enhances phenotype ofcdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic EnhancementPMID:10102358
overexpression rescuescdc37Hsp90 co-chaperone Cdc37 Dosage RescuePMID:16098195
synthetic growth defect withcdc7serine/threonine protein kinase Cdc7 Synthetic Growth DefectPMID:22543982
rescued by overexpression ofcyr1adenylate cyclase Dosage RescuePMID:1849107
synthetic lethal withmyo2myosin II heavy chain Synthetic LethalityPMID:15755919
synthetic growth defect withplo1Polo kinase Plo1 Synthetic Growth DefectPMID:22543982
rescued by overexpression ofptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:8196617
rescued bypyp1tyrosine phosphatase Pyp1 Phenotypic SuppressionPMID:8832414
synthetic lethal withrng3UCS-domain protein Rng3 Synthetic LethalityPMID:15755919
rescued by overexpression ofsck1serine/threonine protein kinase Sck1 Dosage RescuePMID:7498728
synthetic growth defect withsid2NDR kinase Sid2 Synthetic Growth DefectPMID:22543982
External References
Database Identifier Description
NBRP SPAC926.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC926.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC926.04c BioGRID Interaction Datasets
Expression Viewer SPAC926.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC926.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC926.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC926.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC926.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC926.04c Transcriptome Viewer (Bähler Lab)
GEO SPAC926.04c GEO profiles
PInt SPAC926.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC926.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC926.04c Fission yeast phenotypic data & analysis
Cyclebase SPAC926.04c.1 Cell Cycle Data
SPD / RIKEN28/28A02Orfeome Localization Data
UniProtKB/SwissProtP41887Heat shock protein 90 homolog
ModBaseP41887Database of comparative protein structure models
STRINGP41887Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594365Hsp90 chaperone
RefSeq mRNANM_001019786972h- Hsp90 chaperone (hsp90), mRNA
European Nucleotide ArchiveAAC41646.1ENA Protein Mapping
European Nucleotide ArchiveCAB54152.1ENA Protein Mapping
UniParcUPI000012CCBEUniProt Archive

Literature for hsp90

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015