hsp90 (SPAC926.04c)


Gene Standard Namehsp90 Characterisation Statuspublished
Systematic IDSPAC926.04c Feature Typeprotein coding
Synonymsgit10, swo1 Name DescriptionGlucose Insensitive Transcription
ProductHsp90 chaperone Product Size704aa, 80.60 kDa
Genomic Location Chromosome I, 3891157-3888558 (2600nt); CDS:3890888-3888774 (2115nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
ATPase activity235
Annotation ExtensionEvidenceWith/FromReference
protein binding848
Annotation ExtensionEvidenceWith/FromReference
protein binding involved in protein folding1
Annotation ExtensionEvidenceWith/FromReference
unfolded protein binding41
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

protein folding


Term NameCount
'de novo' protein folding6
Annotation ExtensionEvidenceWith/FromReference
cellular response to heat24
Annotation ExtensionEvidenceWith/FromReference
chaperone-mediated protein folding10
Annotation ExtensionEvidenceWith/FromReference
protein folding104
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4206
Annotation ExtensionEvidenceWith/FromReference
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
mitochondrion762
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationhsp90ΔNull1427
swo1-26 (G84C)Not specified
normal vegetative cell population growthgit10-201 (L338P)Not specified685
swo1-26 (G84C)Not specified

Cell Phenotype

Term NameAlleleExpressionCount
abnormal conjugationgit10-201 (L338P)Not specified62
abnormal negative regulation of transcription by glucosegit10-201 (L338P)Not specified9
affecting fbp1git10-201 (L338P)Not specified
abnormal nucleus localization during vegetative growthswo1-21 (L654R)Not specified10
swo1-26 (G84C)Not specified
inviable cell at high temperatureswo1-21 (L654R)Not specified22
swo1-26 (G84C)Not specified
inviable spheroid vegetative cellswo1-26 (G84C)Not specified58
inviable spore467
penetrance FYPO_EXT:0000001hsp90ΔNull
inviable swollen vegetative cellswo1-26 (G84C)Not specified31
vegetative cell lysisswo1-21 (L654R)Not specified82
swo1-26 (G84C)Not specified
viable small vegetative cell38
expressivity FYPO_EXT:0000003swo1-26 (G84C)Not specified
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in lkh1 dual specificity protein kinase Lkh1 PMID:23956636
FYPO affected by mutation in mas5 DNAJ domain protein Mas5 (predicted) PMID:24146635
FYPO affected by mutation in ssa2 heat shock protein Ssa2 PMID:24146635
GO regulated by aha1 chaperone activator Aha1 (predicted) PMID:23664927
GO regulated by sti1 chaperone activator Sti1 (predicted) PMID:23664927
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
138911573888558

UTRs

Region Coordinates Reference
five_prime_UTR3891157..3890889PMID:21511999
three_prime_UTR3888773..3888558PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00183 Pfam IPR001404 Heat shock protein Hsp90 family 184 704 1
PF02518 Pfam IPR003594 Histidine kinase-like ATPase, C-terminal domain 29 161 6
SM00387 SMART IPR003594 Histidine kinase-like ATPase, C-terminal domain 27 165 5
PS00298 Prosite Patterns IPR019805 Heat shock protein Hsp90, conserved site 25 34 1
PTHR11528 HMMPANTHER IPR001404 Heat shock protein Hsp90 family 4 704 1
3.30.565.10 Gene3D IPR003594 Histidine kinase-like ATPase, C-terminal domain 4 212 8
SSF54211 SuperFamily IPR020568 Ribosomal protein S5 domain 2-type fold 267 522 29
0043579 SuperFamily 546 668 1
SSF55874 SuperFamily IPR003594 Histidine kinase-like ATPase, C-terminal domain 4 211 8
PIRSF002583 PIRSF IPR001404 Heat shock protein Hsp90 family 3 696 1
Coil ncoils Rabaptin coiled-coil domain 238 269 971
Coil ncoils Rabaptin coiled-coil domain 529 557 971
Coil ncoils Rabaptin coiled-coil domain 682 703 971
MF_00505 hamap IPR001404 Heat shock protein Hsp90 family 2 669 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 170 187 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 26 48 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 118 140 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 93 110 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 75 92 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 5 25 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 188 206 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.48 Da
Charge -32.50
Isoelectric point 4.60
Molecular weight 80.60 kDa
Number of residues 704
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during cellular response to thiabendazoleS366
present during cellular response to thiabendazole, mitotic M phaseS277
S245, S277, S290, S374, S451
present during cellular response to thiabendazole, mitotic M phaseS245
present during cellular response to thiabendazole, mitotic M phaseS374
present during mitotic M phaseS451
present during mitotic M phaseS290
Annotation ExtensionEvidenceResidueReference
experimental evidence S245 PMID:24763107
present during cellular response to thiabendazole IDA S245 PMID:18257517
present during mitotic M phase experimental evidence S245 PMID:21712547
experimental evidence S277 PMID:24763107
present during mitotic M phase experimental evidence S277 PMID:21712547
present during cellular response to thiabendazole IDA S277 PMID:18257517
experimental evidence S290 PMID:24763107
present during mitotic M phase experimental evidence S290 PMID:21712547
present during cellular response to thiabendazole IDA S366 PMID:18257517
experimental evidence S374 PMID:24763107
present during mitotic M phase experimental evidence S374 PMID:21712547
present during cellular response to thiabendazole IDA S374 PMID:18257517
experimental evidence S451 PMID:24763107
present during mitotic M phase experimental evidence S451 PMID:21712547
O-phospho-L-threonine 693
present during mitotic M phaseT280
present during mitotic M phaseT268
present during mitotic M phaseT292
T68
present during mitotic M phaseT224
Annotation ExtensionEvidenceResidueReference
experimental evidence T68 PMID:24763107
present during mitotic M phase experimental evidence T224 PMID:21712547
present during mitotic M phase experimental evidence T268 PMID:21712547
present during mitotic M phase experimental evidence T280 PMID:21712547
present during mitotic M phase experimental evidence T292 PMID:21712547
phosphorylated residue 1922
Annotation ExtensionEvidenceResidueReference
NAS PMID:18257517
IDA PMID:19547744
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelincreased during GO:0034605Western blot evidencePMID:7813446

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
879705during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
883920during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
886374during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
940434during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
817914during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
284621during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
60584.66during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
160during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
13during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancercancer_census142
Subunit Composition
DescriptionQualifierReferenceCount
homomeric(2) PMID:2366492748
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
chaperone12
Pathway
DescriptionQualifierReferenceCount
PKA PMID:184309262
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
conserved in metazoa3432
conserved in vertebrates3407
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
dcp2mRNA decapping complex catalytic subunit Dcp2 Affinity Capture-MSPMID:23319050
cid13poly(A) polymerase Cid13 Affinity Capture-MSPMID:12062100
myo2myosin II heavy chain Affinity Capture-WesternPMID:15755919
Two-hybrid
aha1chaperone activator Aha1 (predicted) Reconstituted ComplexPMID:23664927
hsp90Hsp90 chaperone Reconstituted ComplexPMID:23664927
cdc37Hsp90 co-chaperone Cdc37 Affinity Capture-WesternPMID:16098195
Co-fractionation
mik1mitotic inhibitor kinase Mik1 Affinity Capture-WesternPMID:11298745
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Affinity Capture-WesternPMID:22543982
lsd1histone demethylase SWIRM1 Affinity Capture-MSPMID:16990277
plo1Polo kinase Plo1 Affinity Capture-WesternPMID:22543982
rng3UCS-domain protein Rng3 Two-hybridPMID:15755919
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
sti1chaperone activator Sti1 (predicted) Reconstituted ComplexPMID:23664927
sog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
slm9hira protein Slm9 Affinity Capture-MSPMID:20976105
wee1M phase inhibitor protein kinase Wee1 Affinity Capture-WesternPMID:7813446
Affinity Capture-WesternPMID:11298745
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
ptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:8196617
myo2myosin II heavy chain Synthetic LethalityPMID:15755919
sck1serine/threonine protein kinase Sck1 Dosage RescuePMID:7498728
cyr1adenylate cyclase Dosage RescuePMID:1849107
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic EnhancementPMID:22543982
Phenotypic EnhancementPMID:10102358
sid2NDR kinase Sid2 Synthetic Growth DefectPMID:22543982
pyp1tyrosine phosphatase Pyp1 Phenotypic SuppressionPMID:8832414
plo1Polo kinase Plo1 Synthetic Growth DefectPMID:22543982
rng3UCS-domain protein Rng3 Synthetic LethalityPMID:15755919
cdc14SIN component Cdc14 Dosage RescuePMID:11791728
cdc37Hsp90 co-chaperone Cdc37 Dosage RescuePMID:16098195
cdc7serine/threonine protein kinase Cdc7 Synthetic Growth DefectPMID:22543982
External References
Database Identifier Description
NBRP SPAC926.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC926.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC926.04c BioGRID Interaction Datasets
Expression Viewer SPAC926.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC926.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC926.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC926.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC926.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC926.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC926.04c Cell Cycle Data
GEO SPAC926.04c GEO profiles
PInt SPAC926.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC926.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC926.04c Fission yeast phenotypic data & analysis
SPD / RIKEN28/28A02Orfeome Localization Data
UniProtKB/SwissProtP41887Heat shock protein 90 homolog
ModBaseP41887Database of comparative protein structure models
STRINGP41887Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594365Hsp90 chaperone
RefSeq mRNANM_001019786972h- Hsp90 chaperone (hsp90), mRNA
European Nucleotide ArchiveAAC41646.1ENA Protein Mapping
European Nucleotide ArchiveCAB54152.1ENA Protein Mapping
UniParcUPI000012CCBEUniProt Archive

Literature for hsp90

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015