hsp90 (SPAC926.04c)


Gene Standard Namehsp90 Characterisation Statuspublished
Systematic IDSPAC926.04c Feature Typeprotein coding
Synonymsgit10, swo1 Name DescriptionGlucose Insensitive Transcription
ProductHsp90 chaperone Product Size704aa, 80.60 kDa
Genomic Location Chromosome I, 3891157-3888558 (2600nt); CDS:3890888-3888774 (2115nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIDAPMID:23664927558
GO:0016887ATPase activityIDAPMID:23664927232
GO:0042623ATPase activity, coupledISOSGD:S000006161GO_REF:0000024171
GO:0005515protein bindingIPIaha1PMID:23664927831
IPIsti1PMID:23664927
GO:0044183protein binding involved in protein foldingEXPPMID:184309261
GO:0051082unfolded protein bindingIDAPMID:2366492742
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006458'de novo' protein foldingISOSGD:S000006161GO_REF:00000246
GO:0034605cellular response to heatISOSGD:S000004798GO_REF:000002426
GO:0061077chaperone-mediated protein foldingNASGO_REF:000000110
GO:0006457protein foldingIMPPMID:11791728106
EXPPMID:18430926
IDAPMID:23664927
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISOSGD:S000004798GO_REF:00000244205
GO:0005829cytosolIDAPMID:168233722315
GO:0005739mitochondrionISOSGD:S000004798GO_REF:0000024758
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyhsp90ΔNullPMID:204732891338
Microscopyhsp90ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0001357normal vegetative cell population growthCell growth assaygit10-201 (L338P)Not specifiedPECO:0000005PMID:18430926641
Cell growth assaygit10-201 (L338P)Not specifiedPECO:0000004PMID:18430926

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000031abnormal conjugationMicroscopygit10-201 (L338P)Not specifiedPMID:1843092687
FYPO:0000044abnormal negative regulation of transcription by glucose9
affecting fbp1Transcript expression level evidencegit10-201 (L338P)Not specifiedPECO:0000162, PECO:0000056PMID:1849107
affecting fbp1Transcript expression level evidencegit10-201 (L338P)Not specifiedPECO:0000162PMID:1849107
Reporter gene assaygit10-201 (L338P)Not specifiedPMID:18430926
FYPO:0001338abnormal nucleus localization during vegetative growthMicroscopyswo1-21 (L654R)Not specifiedPECO:0000004, PECO:0000126PMID:1843092610
Microscopyswo1-26 (G84C)Not specifiedPECO:0000004, PECO:0000126PMID:18430926
FYPO:0001801inviable cell at high temperatureCell growth assayswo1-26 (G84C)Not specifiedPMID:1843092623
Cell growth assayswo1-21 (L654R)Not specifiedPMID:18430926
FYPO:0002151inviable spore465
penetrance FYPO_EXT:0000001Microscopyhsp90ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0000023small cellMicroscopynot recorded (unrecorded)PMID:781344672
FYPO:0000647vegetative cell lysisMicroscopyswo1-21 (L654R)Not specifiedPECO:0000004, PECO:0000126PMID:1843092680
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in lkh1 dual specificity protein kinase Lkh1 PMID:23956636
FYPO affected by mutation in mas5 DNAJ domain protein Mas5 (predicted) PMID:24146635
FYPO affected by mutation in ssa2 heat shock protein Ssa2 PMID:24146635
GO regulated by aha1 chaperone activator Aha1 (predicted) PMID:23664927
GO regulated by sti1 chaperone activator Sti1 (predicted) PMID:23664927
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
138911573888558

UTRs

Region Coordinates Reference
five_prime_UTR3891157..3890889PMID:21511999
three_prime_UTR3888773..3888558PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00183 Pfam IPR001404 Heat shock protein Hsp90 family 184 704 1
PF02518 Pfam IPR003594 Histidine kinase-like ATPase, ATP-binding domain 29 161 6
SM00387 SMART IPR003594 Histidine kinase-like ATPase, ATP-binding domain 27 165 5
PS00298 Prosite Patterns IPR019805 Heat shock protein Hsp90, conserved site 25 34 1
PTHR11528 HMMPANTHER IPR001404 Heat shock protein Hsp90 family 4 704 1
3.30.565.10 Gene3D IPR003594 Histidine kinase-like ATPase, ATP-binding domain 4 212 8
0043579 SuperFamily 546 668 1
SSF54211 SuperFamily IPR020568 Ribosomal protein S5 domain 2-type fold 267 522 29
SSF55874 SuperFamily IPR003594 Histidine kinase-like ATPase, ATP-binding domain 4 211 8
PIRSF002583 PIRSF IPR001404 Heat shock protein Hsp90 family 3 696 1
Coil ncoils Rabaptin coiled-coil domain 529 557 968
Coil ncoils Rabaptin coiled-coil domain 682 703 968
Coil ncoils Rabaptin coiled-coil domain 238 269 968
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 188 206 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 26 48 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 75 92 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 93 110 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 118 140 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 170 187 1
PR00775 PRINTS IPR020575 Heat shock protein Hsp90, N-terminal 5 25 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.48 Da
Charge -32.50
Isoelectric point 4.60
Molecular weight 80.60 kDa
Number of residues 704
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS245PMID:217125471670
present during mitotic M phase
present during mitotic M phaseexperimental evidenceS451PMID:21712547
present during mitotic M phaseexperimental evidenceS277PMID:21712547
present during mitotic M phaseexperimental evidenceS290PMID:21712547
experimental evidenceS451PMID:24763107
present during mitotic M phaseexperimental evidenceS374PMID:21712547
experimental evidenceS245PMID:24763107
experimental evidenceS290PMID:24763107
experimental evidenceS374PMID:24763107
experimental evidenceS277PMID:24763107
present during cellular response to thiabendazoleIDAS366PMID:18257517
present during cellular response to thiabendazoleIDAS374PMID:18257517
present during cellular response to thiabendazoleIDAS245PMID:18257517
present during cellular response to thiabendazoleIDAS277PMID:18257517
MOD:00047O-phospho-L-threonineexperimental evidenceT292PMID:21712547692
present during mitotic M phase
present during mitotic M phaseexperimental evidenceT224PMID:21712547
present during mitotic M phaseexperimental evidenceT280PMID:21712547
present during mitotic M phaseexperimental evidenceT268PMID:21712547
experimental evidenceT68PMID:24763107
MOD:00696phosphorylated residueNASPMID:195477441922
NASPMID:18257517
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
879705during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
883920during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
886374during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
940434during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
817914during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
284621during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
60584.66during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
160during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
13during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancercancer_census142
Subunit Composition
DescriptionQualifierReferenceCount
homomeric(2) PMID:2366492746
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
chaperone12
Pathway
DescriptionQualifierReferenceCount
PKA PMID:184309262
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3425
conserved in vertebrates3400
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:8196617
myo2myosin II heavy chain Synthetic LethalityPMID:15755919
sck1serine/threonine protein kinase Sck1 Dosage RescuePMID:7498728
cyr1adenylate cyclase Dosage RescuePMID:1849107
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic EnhancementPMID:22543982
Phenotypic EnhancementPMID:10102358
sid2NDR kinase Sid2 Synthetic Growth DefectPMID:22543982
pyp1tyrosine phosphatase Pyp1 Phenotypic SuppressionPMID:8832414
plo1Polo kinase Plo1 Synthetic Growth DefectPMID:22543982
rng3UCS-domain protein Rng3 Synthetic LethalityPMID:15755919
cdc14SIN component Cdc14 Dosage RescuePMID:11791728
cdc37Hsp90 co-chaperone Cdc37 Dosage RescuePMID:16098195
cdc7serine/threonine protein kinase Cdc7 Synthetic Growth DefectPMID:22543982
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
dcp2mRNA decapping complex catalytic subunit Dcp2 Affinity Capture-MSPMID:23319050
cid13poly(A) polymerase Cid13 Affinity Capture-MSPMID:12062100
myo2myosin II heavy chain Two-hybridPMID:15755919
Affinity Capture-Western
aha1chaperone activator Aha1 (predicted) Reconstituted ComplexPMID:23664927
hsp90Hsp90 chaperone Reconstituted ComplexPMID:23664927
cdc37Hsp90 co-chaperone Cdc37 Affinity Capture-WesternPMID:16098195
Co-fractionation
mik1mitotic inhibitor kinase Mik1 Affinity Capture-WesternPMID:11298745
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Affinity Capture-WesternPMID:22543982
lsd1histone demethylase SWIRM1 Affinity Capture-MSPMID:16990277
plo1Polo kinase Plo1 Affinity Capture-WesternPMID:22543982
rng3UCS-domain protein Rng3 Two-hybridPMID:15755919
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
sti1chaperone activator Sti1 (predicted) Reconstituted ComplexPMID:23664927
sog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
slm9hira protein Slm9 Affinity Capture-MSPMID:20976105
wee1M phase inhibitor protein kinase Wee1 Affinity Capture-WesternPMID:11298745
External References
Database Identifier Description
NBRP SPAC926.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC926.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC926.04c BioGRID Interaction Datasets
Expression Viewer SPAC926.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC926.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC926.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC926.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC926.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC926.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC926.04c Cell Cycle Data
GEO SPAC926.04c GEO profiles
PInt SPAC926.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC926.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC926.04c Fission yeast phenotypic data & analysis
SPD / RIKEN28/28A02Orfeome Localization Data
UniProtKB/SwissProtP41887Heat shock protein 90 homolog
ModBaseP41887Database of comparative protein structure models
STRINGP41887Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594365Hsp90 chaperone
RefSeq mRNANM_001019786972h- Hsp90 chaperone (hsp90), mRNA
European Nucleotide ArchiveAAC41646.1ENA Protein Mapping
European Nucleotide ArchiveCAB54152.1ENA Protein Mapping
UniParcUPI000012CCBEUniProt Archive

Literature for hsp90

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014