rpl3001 (SPAC9G1.03c)


Gene Standard Namerpl3001 Characterisation Statusbiological_role_inferred
Systematic IDSPAC9G1.03c Feature Typeprotein coding
Synonymsrpl30, rpl30-1 Name Description
Product60S ribosomal protein L30 (predicted) Product Size109aa, 11.59 kDa
Genomic Location Chromosome I, 1974092-1973479 (614nt); CDS:1974054-1973666 (389nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003735structural constituent of ribosomeISOSGD:S000002998GO_REF:0000024255
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0002181cytoplasmic translationNASGO_REF:0000051445
GO:0006364rRNA processingISOSGD:S000002998GO_REF:0000024181
GO:0006412translationISOSGD:S000002998GO_REF:0000024597
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722316
GO:0022625cytosolic large ribosomal subunitISOSGD:S000002998GO_REF:000002482
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000086sensitive to tacrolimus81
expressivity FYPO_EXT:0000003Cell growth assayrpl3001ΔNullPECO:0000201, PECO:0000005, PECO:0000142PMID:21850271
FYPO:0002060viable vegetative cell populationMicroscopyrpl3001ΔNullPMID:204732893760
Microscopyrpl3001ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002398abnormal actin cytoskeletonMicroscopyrpl3001ΔNullPMID:2369780643
FYPO:0002399abnormal microtubule cytoskeletonMicroscopyrpl3001ΔNullPMID:2369780632
FYPO:0001018abolished activation of bipolar cell growthMicroscopyrpl3001ΔNullPMID:2369780611
FYPO:0002937decreased pre-mRNA level1
affecting rpl3002Transcript expression level evidencerpl3001::kanMX6 (disruption)Not specifiedPMID:24081329
FYPO:0002400single microtubule bundleMicroscopyrpl3001ΔNullPMID:2369780610
FYPO:0001510viable vegetative cell, abnormal cell shape, normal cell size215
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000001
Microscopyrpl3001ΔNullPECO:0000137, PECO:0000005PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
119740921973816
219737561973479

UTRs

Region Coordinates Reference
five_prime_UTR1974092..1974055PMID:21511999
three_prime_UTR1973665..1973479PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01248 Pfam IPR004038 Ribosomal protein L7Ae/L30e/S12e/Gadd45 15 107 7
PS00993 Prosite Patterns IPR022991 Ribosomal protein L30e, conserved site 82 102 2
PS00709 Prosite Patterns IPR022991 Ribosomal protein L30e, conserved site 26 50 2
PTHR11449:SF1 HMMPANTHER 3 109 2
PTHR11449 HMMPANTHER IPR000231 Ribosomal protein L30e 3 109 2
3.30.1330.30 Gene3D IPR029064 50S ribosomal protein L30e-like 8 107 10
SSF55315 SuperFamily IPR029064 50S ribosomal protein L30e-like 15 107 10

View domain organization at Pfam

Protein Properties

Ave. residue weight 106.35 Da
Charge 10.00
Isoelectric point 10.25
Molecular weight 11.59 kDa
Number of residues 109
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residueNASPMID:195477441915
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
228835during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
266670during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
276697during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
251282during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
258070during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
138909.29during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
48435.93during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
62during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
5.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3393
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
pmc1mediator complex subunit Pmc1 Negative GeneticPMID:22681890
duo1DASH complex subunit Duo1 Negative GeneticPMID:22681890
cwf18complexed with Cdc5 protein Cwf18 Negative GeneticPMID:22681890
dad1DASH complex subunit Dad1 Negative GeneticPMID:22681890
cwf28splicing factor Cwf28 Negative GeneticPMID:22681890
cdr2serine/threonine protein kinase Cdr2 Negative GeneticPMID:22681890
srp1SR family protein, human SRFS2 ortholog Srp1 Negative GeneticPMID:22681890
SPBC56F2.08cRNA-binding protein (predicted) Negative GeneticPMID:22681890
SPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:22681890
aar2U5 snRNP-associated protein Aar2 (predicted) Positive GeneticPMID:22681890
ask1DASH complex subunit Ask1 Negative GeneticPMID:22681890
dad4DASH complex subunit Dad4 Negative GeneticPMID:22681890
spc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
SPBC29A3.09cAAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
not2CCR4-Not complex subunit Not2 (predicted) Negative GeneticPMID:22681890
mug154conserved fungal protein Negative GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
int6eIF3e subunit Int6 Negative GeneticPMID:22681890
swr1SNF2 family helicase Swr1 Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
sif1Sad1 interacting factor 1 Positive GeneticPMID:22681890
pfd1prefoldin subunit 1 (predicted) Negative GeneticPMID:22681890
prp19ubiquitin-protein ligase E4 Negative GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:22681890
elp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
SPAC1952.06cspliceosomal complex subunit (predicted) Negative GeneticPMID:22681890
dam1DASH complex subunit Dam1 Negative GeneticPMID:22681890
sdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cid14poly(A) polymerase Cid14 Affinity Capture-MSPMID:20403971
fic1C2 domain protein Fic1 Affinity Capture-MSPMID:19139265
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
sog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
pab2poly(A) binding protein Pab2 Affinity Capture-MSPMID:19336419
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPAC9G1.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC9G1.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC9G1.03c BioGRID Interaction Datasets
Expression Viewer SPAC9G1.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC9G1.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC9G1.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC9G1.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC9G1.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC9G1.03c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC9G1.03c Cell Cycle Data
GEO SPAC9G1.03c GEO profiles
PInt SPAC9G1.03c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene254284860S ribosomal protein L30
EntrezGene254284860S ribosomal protein L30
SPD / RIKEN02/02H06Orfeome Localization Data
UniProtKB/SwissProtP5280860S ribosomal protein L30-1
ModBaseP52808Database of comparative protein structure models
STRINGP52808Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_59355860S ribosomal protein L30
RefSeq mRNANM_001018991972h- 60S ribosomal protein L30 (rpl3001), mRNA
European Nucleotide ArchiveU97398ENA EMBL mapping
European Nucleotide ArchiveAAB17132ENA Protein Mapping
European Nucleotide ArchiveAAB17132.1ENA Protein Mapping
European Nucleotide ArchiveAAB63890ENA Protein Mapping
European Nucleotide ArchiveCAB11499ENA Protein Mapping
European Nucleotide ArchiveCAB11499.1ENA Protein Mapping
UniParcUPI0000132F9BUniProt Archive

Literature for rpl3001

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014