aip1 (SPAC9G1.05)


Gene Standard Nameaip1 Characterisation Statuspublished
Systematic IDSPAC9G1.05 Feature Typeprotein coding
Synonyms Name Description
Productactin cortical patch component Aip1 Product Size595aa, 65.27 kDa
Genomic Location Chromosome I, 1976706-1979209 (2504nt); CDS:1976902-1979061 (2160nt)

Ensembl Gene Location
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030042actin filament depolymerizationISOSGD:S000004698GO_REF:00000246
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030479actin cortical patchISOSGD:S000004698GO_REF:000002447
GO:0032153cell division siteIDAPMID:16823372301
GO:0051286cell tipIDAPMID:16823372190
GO:0005737cytoplasmISOSGD:S000004698GO_REF:00000244205
GO:0005829cytosolIDAPMID:168233722315
GO:0005634nucleusISOSGD:S000004698GO_REF:00000242740
IDAPMID:16823372
GO:0043234protein complexNASGO_REF:00000511363
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000088sensitive to hydroxyureacompetitive growth assay evidenceaip1ΔNullPMID:20537132514
expressivity FYPO_EXT:0000003Cell growth assayaip1ΔNullPECO:0000137, PECO:0000005PMID:19264558
expressivity FYPO_EXT:0000003Cell Growth Assayaip1ΔNullPECO:0000137, PECO:0000102PMID:23697806
FYPO:0002060viable vegetative cell populationMicroscopyaip1ΔNullPECO:0000005, PECO:0000137PMID:236978063759
Microscopyaip1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopyaip1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
119767061977006
219770681977191
319772621977363
419774041977449
519775561977601
619776511978592
719786391979209

UTRs

Region Coordinates Reference
five_prime_UTR1976706..1976901PMID:21511999
three_prime_UTR1979062..1979209PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00400 Pfam IPR001680 WD40 repeat 54 86 104
PF00400 Pfam IPR001680 WD40 repeat 222 259 104
PF00400 Pfam IPR001680 WD40 repeat 306 343 104
PF00400 Pfam IPR001680 WD40 repeat 474 507 104
PF00400 Pfam IPR001680 WD40 repeat 521 550 104
PF00400 Pfam IPR001680 WD40 repeat 183 217 104
SM00320 SMART IPR001680 WD40 repeat 220 259 112
SM00320 SMART IPR001680 WD40 repeat 554 592 112
SM00320 SMART IPR001680 WD40 repeat 419 463 112
SM00320 SMART IPR001680 WD40 repeat 468 507 112
SM00320 SMART IPR001680 WD40 repeat 347 384 112
SM00320 SMART IPR001680 WD40 repeat 47 86 112
SM00320 SMART IPR001680 WD40 repeat 304 343 112
SM00320 SMART IPR001680 WD40 repeat 178 217 112
SM00320 SMART IPR001680 WD40 repeat 135 175 112
SM00320 SMART IPR001680 WD40 repeat 511 550 112
PS50082 Prosite Profiles IPR001680 WD40 repeat 311 344 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 185 226 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 561 595 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 475 516 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 54 86 100
PS50294 Prosite Profiles IPR017986 WD40-repeat-containing domain 54 595 104
PS50082 Prosite Profiles IPR001680 WD40 repeat 227 268 100
PTHR19856 HMMPANTHER 1 595 1
2.130.10.10 Gene3D IPR015943 WD40/YVTN repeat-like-containing domain 54 388 145
2.130.10.10 Gene3D IPR015943 WD40/YVTN repeat-like-containing domain 419 593 145
SSF50978 SuperFamily IPR017986 WD40-repeat-containing domain 26 343 135
SSF50978 SuperFamily IPR017986 WD40-repeat-containing domain 422 593 135
SSF50978 SuperFamily IPR017986 WD40-repeat-containing domain 283 389 135

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000190WD repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000190

Protein Properties

Ave. residue weight 109.69 Da
Charge 0.00
Isoelectric point 6.50
Molecular weight 65.27 kDa
Number of residues 595
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
41607during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
43225during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
38781during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
39896during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
19028.57during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
40522during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
17904.61during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.9during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
lub1WD repeat protein Lub1 Negative GeneticPMID:22681890
prp4serine/threonine protein kinase Prp4 Positive GeneticPMID:22681890
SPAC29A4.02ctranslation elongation factor EF-1 gamma subunit Negative GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
clp1Cdc14-related protein phosphatase Clp1/Flp1 Phenotypic EnhancementPMID:21931816
acp2F-actin capping protein beta subunit Acp2 Synthetic LethalityPMID:25143407
raf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
acp1F-actin capping protein alpha subunit Phenotypic EnhancementPMID:25143407
Synthetic Growth Defect
sre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
atp11F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:22681890
pob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
adf1actin depolymerizing factor, cofilin Synthetic LethalityPMID:22024167
res2MBF transcription factor complex subunit Res2 Negative GeneticPMID:22681890
trt1telomerase reverse transcriptase 1 protein Trt1 Negative GeneticPMID:22681890
sfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
SPBC21C3.03ABC1 kinase family protein Negative GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Positive GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
vps5retromer complex subunit Vps5 Positive GeneticPMID:22681890
elp2elongator complex subunit Elp2 (predicted) Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cid13poly(A) polymerase Cid13 Affinity Capture-MSPMID:12062100
mtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
pir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
red1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
External References
Database Identifier Description
NBRP SPAC9G1.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC9G1.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC9G1.05 BioGRID Interaction Datasets
Expression Viewer SPAC9G1.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC9G1.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC9G1.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC9G1.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC9G1.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC9G1.05 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC9G1.05 Cell Cycle Data
GEO SPAC9G1.05 GEO profiles
PInt SPAC9G1.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC9G1.05 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC9G1.05 Fission yeast phenotypic data & analysis
SPD / RIKEN37/37F11Orfeome Localization Data
UniProtKB/SwissProtO14301Uncharacterized WD repeat-containing protein C9G1.05
ModBaseO14301Database of comparative protein structure models
STRINGO14301Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593560actin cortical patch component Aip1 (predicted)
RefSeq mRNANM_001018993972h- actin cortical patch component Aip1 (predicted) (SPAC9G1.05), mRNA
European Nucleotide ArchiveCAB11489.1ENA Protein Mapping
UniParcUPI000013AB52UniProt Archive

Literature for aip1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014