aip1 (SPAC9G1.05)


Gene Standard Nameaip1 Characterisation Statuspublished
Systematic IDSPAC9G1.05 Feature Typeprotein coding
Synonyms Name Description
Productactin cortical patch component Aip1 Product Size595aa, 65.27 kDa
Genomic Location Chromosome I, 1976706-1979209 (2504nt); CDS:1976902-1979061 (2160nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
actin filament depolymerization6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cortical patch47
Annotation ExtensionEvidenceWith/FromReference
cell division site305
Annotation ExtensionEvidenceWith/FromReference
cell tip194
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4208
Annotation ExtensionEvidenceWith/FromReference
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
protein complex1370
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
sensitive to hydroxyureaaip1ΔNull525
viable vegetative cell populationaip1ΔNull3792

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologyaip1ΔNull3092
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in rpb1 RNA polymerase II large subunit Rpb1 PMID:21931816
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1976706..1977006, 1977068..1977191, 1977262..1977363, 1977404..1977449, 1977556..1977601, 1977651..1978592, 1978639..1979209
mRNA1976706..1979209
5' UTR1976706..1976901PMID:21511999
CDS1976902..1977006, 1977068..1977191, 1977262..1977363, 1977404..1977449, 1977556..1977601, 1977651..1978592, 1978639..1979061
3' UTR1979062..1979209PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00400 Pfam IPR001680 WD40 repeat 521 550 104
PF00400 Pfam IPR001680 WD40 repeat 54 86 104
PF00400 Pfam IPR001680 WD40 repeat 183 217 104
PF00400 Pfam IPR001680 WD40 repeat 474 507 104
PF00400 Pfam IPR001680 WD40 repeat 306 343 104
PF00400 Pfam IPR001680 WD40 repeat 222 259 104
SM00320 SMART IPR001680 WD40 repeat 511 550 112
SM00320 SMART IPR001680 WD40 repeat 135 175 112
SM00320 SMART IPR001680 WD40 repeat 178 217 112
SM00320 SMART IPR001680 WD40 repeat 47 86 112
SM00320 SMART IPR001680 WD40 repeat 304 343 112
SM00320 SMART IPR001680 WD40 repeat 347 384 112
SM00320 SMART IPR001680 WD40 repeat 468 507 112
SM00320 SMART IPR001680 WD40 repeat 419 463 112
SM00320 SMART IPR001680 WD40 repeat 554 592 112
SM00320 SMART IPR001680 WD40 repeat 220 259 112
PS50294 Prosite Profiles IPR017986 WD40-repeat-containing domain 54 595 104
PS50082 Prosite Profiles IPR001680 WD40 repeat 185 226 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 311 344 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 227 268 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 54 86 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 561 595 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 475 516 100
PTHR19856 HMMPANTHER 1 595 1
PTHR19856:SF0 HMMPANTHER 1 595 1
2.130.10.10 Gene3D IPR015943 WD40/YVTN repeat-like-containing domain 419 593 145
2.130.10.10 Gene3D IPR015943 WD40/YVTN repeat-like-containing domain 54 388 145
SSF50978 SuperFamily IPR017986 WD40-repeat-containing domain 283 389 135
SSF50978 SuperFamily IPR017986 WD40-repeat-containing domain 26 343 135
SSF50978 SuperFamily IPR017986 WD40-repeat-containing domain 422 593 135

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000190WD repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000190

Protein Properties

Ave. residue weight 109.69 Da
Charge 0.00
Isoelectric point 6.50
Molecular weight 65.27 kDa
Number of residues 595
Modifications

Protein Modifications

Term NameResidueCount
phosphorylated residue 1926
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
41607during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
43225during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
38781during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
39896during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
19028.57during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
40522during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
17904.61during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.9during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured bycid13poly(A) polymerase Cid13 Affinity Capture-MSPMID:12062100
affinity captured bymtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
affinity captured byred1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withacp1F-actin capping protein alpha subunit Synthetic Growth DefectPMID:25143407
phenotype enhanced byacp1F-actin capping protein alpha subunit Phenotypic EnhancementPMID:25143407
synthetic lethal withacp2F-actin capping protein beta subunit Acp2 Synthetic LethalityPMID:25143407
synthetic lethal withadf1actin depolymerizing factor, cofilin Synthetic LethalityPMID:22024167
negative genetic interaction withatp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:22681890
phenotype enhanced byclp1Cdc14-related protein phosphatase Clp1/Flp1 Phenotypic EnhancementPMID:21931816
negative genetic interaction withelp2elongator complex subunit Elp2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withlub1WD repeat protein Lub1 Negative GeneticPMID:22681890
positive genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
negative genetic interaction withpob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
positive genetic interaction withprp4serine/threonine protein kinase Prp4 Positive GeneticPMID:22681890
positive genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Positive GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withraf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withres2MBF transcription factor complex subunit Res2 Negative GeneticPMID:22681890
negative genetic interaction withsfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC29A4.02ctranslation elongation factor EF-1 gamma subunit Negative GeneticPMID:22681890
negative genetic interaction withSPBC21C3.03ABC1 kinase family protein Negative GeneticPMID:22681890
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
negative genetic interaction withtrt1telomerase reverse transcriptase 1 protein Trt1 Negative GeneticPMID:22681890
positive genetic interaction withvps5retromer complex subunit Vps5 Positive GeneticPMID:22681890
positive genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC9G1.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC9G1.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC9G1.05 BioGRID Interaction Datasets
Expression Viewer SPAC9G1.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC9G1.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC9G1.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC9G1.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC9G1.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC9G1.05 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC9G1.05 Cell Cycle Data
GEO SPAC9G1.05 GEO profiles
PInt SPAC9G1.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC9G1.05 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC9G1.05 Fission yeast phenotypic data & analysis
SPD / RIKEN37/37F11Orfeome Localization Data
UniProtKB/SwissProtO14301Uncharacterized WD repeat-containing protein C9G1.05
ModBaseO14301Database of comparative protein structure models
STRINGO14301Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593560actin cortical patch component Aip1 (predicted)
RefSeq mRNANM_001018993972h- actin cortical patch component Aip1 (predicted) (SPAC9G1.05), mRNA
European Nucleotide ArchiveCAB11489.1ENA Protein Mapping
UniParcUPI000013AB52UniProt Archive

Literature for aip1

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015