cyk3 (SPAC9G1.06c)


Gene Standard Namecyk3 Characterisation Statusbiological role inferred
Systematic IDSPAC9G1.06c Feature Typeprotein coding
Synonyms Name Description
Productcytokinesis protein Cyk3 (predicted) Product Size886aa, 98.26 kDa
Genomic Location Chromosome I, 1982206-1976754 (5453nt); CDS:1982107-1979364 (2744nt)

Ensembl Gene Location
GO Biological Process

GO Slim Terms

mitotic cytokinesis


Term NameCount
mitotic cytokinesis104
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actomyosin contractile ring39
Annotation ExtensionEvidenceWith/FromReference
cell division site317
Annotation ExtensionEvidenceWith/FromReference
cell tip209
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationcyk3Δ3850

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell, abnormal cell shape, normal cell sizecyk3Δ215
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in pom1 DYRK family protein kinase Pom1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1982206..1982041, 1981957..1976754
Intron1982040..1981958
mRNA1982206..1976754
5' UTR1982206..1982108PMID:21511999
CDS1982107..1982041, 1981957..1979364
3' UTR1979363..1976754PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00018 Pfam IPR001452 SH3 domain 12 59 20
SM00326 SMART IPR001452 SH3 domain 9 66 21
SM00460 SMART IPR002931 Transglutaminase-like 528 595 2
PS50002 Prosite Profiles IPR001452 SH3 domain 6 67 21
PTHR11039 HMMPANTHER 456 689 1
PTHR11039:SF41 HMMPANTHER 456 689 1
2.30.30.40 Gene3D CheW-like domain 4 63 21
SSF54001 SuperFamily 494 637 30
SSF50044 SuperFamily IPR001452 SH3 domain 5 87 21

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.91 Da
Charge 31.50
Codon Adaptation Index 0.35
Isoelectric point 9.75
Molecular weight 98.26 kDa
Number of residues 886
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS207
present during mitotic M phaseS213
present during mitotic M phaseS137
present during mitotic M phaseS390
present during mitotic M phaseS140
present during mitotic M phaseS261
present during mitotic M phaseS141
present during mitotic M phaseS125
present during mitotic M phaseS209
present during mitotic M phaseS143
present during mitotic M phaseS131
present during mitotic M phaseS123
present during mitotic M phaseS337
present during mitotic M phaseS341
present during mitotic M phaseS267
S112, S140, S143, S174, S209, S213, S261, S267, S280, S283, S284, S300, S310, S390
level fluctuates during mitotic cell cycleS267
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S207 PMID:21712547
present during mitotic M phase experimental evidence S213 PMID:21712547
present during mitotic M phase experimental evidence S137 PMID:21712547
present during mitotic M phase experimental evidence S390 PMID:21712547
present during mitotic M phase experimental evidence S140 PMID:21712547
present during mitotic M phase experimental evidence S261 PMID:21712547
present during mitotic M phase experimental evidence S141 PMID:21712547
present during mitotic M phase experimental evidence S125 PMID:21712547
present during mitotic M phase experimental evidence S209 PMID:21712547
present during mitotic M phase experimental evidence S143 PMID:21712547
present during mitotic M phase experimental evidence S131 PMID:21712547
present during mitotic M phase experimental evidence S123 PMID:21712547
present during mitotic M phase experimental evidence S337 PMID:21712547
present during mitotic M phase experimental evidence S341 PMID:21712547
present during mitotic M phase experimental evidence S267 PMID:21712547
IDA S261 PMID:25720772
IDA S174 PMID:25720772
level fluctuates during mitotic cell cycle experimental evidence S267 PMID:24763107
experimental evidence S390 PMID:24763107
IDA S310 PMID:25720772
IDA S267 PMID:25720772
IDA S390 PMID:25720772
IDA S143 PMID:25720772
IDA S284 PMID:25720772
IDA S280 PMID:25720772
IDA S213 PMID:25720772
IDA S267 PMID:23297348
IDA S300 PMID:25720772
experimental evidence S209 PMID:24763107
IDA S112 PMID:25720772
IDA S140 PMID:25720772
IDA S283 PMID:25720772
experimental evidence S143 PMID:24763107
experimental evidence S213 PMID:24763107
O-phospho-L-threonine 1085
present during mitotic M phaseT393
present during mitotic M phaseT260
present during mitotic M phaseT332
present during mitotic M phaseT219
present during mitotic M phaseT338
T260, T332, T406
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T393 PMID:21712547
present during mitotic M phase experimental evidence T260 PMID:21712547
present during mitotic M phase experimental evidence T332 PMID:21712547
present during mitotic M phase experimental evidence T219 PMID:21712547
present during mitotic M phase experimental evidence T338 PMID:21712547
experimental evidence T332 PMID:24763107
IDA T406 PMID:25720772
IDA T260 PMID:25720772
O-phosphorylated residueS137,S140,S141, S137,S140,S143, S137,S143, S140,S141, S140,S141,S143, S140,S143, S141,S143, S147,S153, S147,S153,S154, S147,S153,S156, S147,S154,S156, S149,S153,S156, S149,S154, S149,S154,S156, S153,S154, S153,S156, S154,S156, S156,T158, S174,S182,S187, S184,S187, S184,S191, S207,S209,S213, S207,S213, S209,S213, S261,S267, S267,S271, S267,S272, S276,S280, S276,S280,S283, S280,S283, S280,S283,S284, S280,S284, S300,S310, S300,T311, S337,S341, T79,S80, T79,S82, T79,S83, T106,S112, T185,S187, T260,S271, T320,T332,S337, T332,S337, T332,S337,S341, T332,S341, T332,T333, T332,T333,S341, T332,T338, T332,T338,S341, T338,S341 2457
Annotation ExtensionEvidenceResidueReference
IDA S137,S140,S141 PMID:25720772
IDA S153,S156 PMID:25720772
IDA S300,T311 PMID:25720772
IDA S140,S143 PMID:25720772
IDA S140,S141,S143 PMID:25720772
IDA T338,S341 PMID:25720772
IDA S300,S310 PMID:25720772
IDA S147,S153,S156 PMID:25720772
IDA S276,S280 PMID:25720772
IDA S280,S283 PMID:25720772
IDA T260,S271 PMID:25720772
IDA S137,S143 PMID:25720772
IDA T332,T333,S341 PMID:25720772
IDA T332,S341 PMID:25720772
IDA S184,S187 PMID:25720772
IDA S137,S140,S143 PMID:25720772
IDA S184,S191 PMID:25720772
IDA S140,S141 PMID:25720772
IDA T332,S337,S341 PMID:25720772
IDA T185,S187 PMID:25720772
IDA T332,T333 PMID:25720772
IDA S147,S154,S156 PMID:25720772
IDA S280,S283,S284 PMID:25720772
IDA S147,S153,S154 PMID:25720772
IDA S149,S154 PMID:25720772
IDA S207,S213 PMID:25720772
IDA T79,S80 PMID:25720772
IDA S149,S153,S156 PMID:25720772
IDA S207,S209,S213 PMID:25720772
IDA S149,S154,S156 PMID:25720772
IDA S154,S156 PMID:25720772
IDA T332,T338,S341 PMID:25720772
IDA T79,S83 PMID:25720772
IDA S153,S154 PMID:25720772
IDA S276,S280,S283 PMID:25720772
IDA S141,S143 PMID:25720772
IDA T106,S112 PMID:25720772
IDA S174,S182,S187 PMID:25720772
IDA T320,T332,S337 PMID:25720772
IDA S280,S284 PMID:25720772
IDA S147,S153 PMID:25720772
IDA S261,S267 PMID:25720772
IDA T332,S337 PMID:25720772
IDA T332,T338 PMID:25720772
IDA T79,S82 PMID:25720772
IDA S267,S271 PMID:25720772
IDA S156,T158 PMID:25720772
IDA S267,S272 PMID:25720772
IDA S337,S341 PMID:25720772
IDA S209,S213 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for cyk3 (SPAC9G1.06c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1871during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2237during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1961during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2333during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3103.53during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1264during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4039.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.1during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.84during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi only616
conserved in fungi4607
conserved in eukaryotes4517
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC9G1.06c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesmyo1myosin type I Affinity Capture-MSPMID:20603077
affinity capturesshm2serine hydroxymethyltransferase Shm2 (predicted) Affinity Capture-MSPMID:20603077
affinity capturesala1mitochondrial and cytoplasmic alanine-tRNA ligase Ala1 (predicted) Affinity Capture-MSPMID:20603077
affinity capturesrad2514-3-3 protein Rad25 Affinity Capture-MSPMID:20603077
affinity capturesrad2414-3-3 protein Rad24 Affinity Capture-MSPMID:20603077
affinity capturesgpd1glycerol-3-phosphate dehydrogenase Gpd1 Affinity Capture-MSPMID:20603077
affinity captureshhf1histone H4 h4.1 Affinity Capture-MSPMID:20603077
affinity capturesfic1C2 domain protein Fic1 Affinity Capture-WesternPMID:23093943
affinity capturessec21coatomer gamma subunit Sec21 (predicted) Affinity Capture-MSPMID:20603077
affinity capturesaco1aconitate hydratase Aco1 (predicted) Affinity Capture-MSPMID:20603077
affinity capturesapl2AP-1 adaptor complex subunit beta subunit Apl2 Affinity Capture-MSPMID:20603077
affinity capturesnrs1cytoplasmic asparagine-tRNA ligase Nrs1 (predicted) Affinity Capture-MSPMID:20603077
affinity capturescdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Affinity Capture-MSPMID:20603077
affinity capturesnog1GTP binding protein Nog1 (predicted) Affinity Capture-MSPMID:20603077
affinity capturestif38translation initiation factor eIF3h (p40) Affinity Capture-MSPMID:20603077
affinity capturestif313translation initiation factor eIF3m Affinity Capture-MSPMID:20603077
affinity capturesmug64BAR domain protein (predicted) Affinity Capture-MSPMID:20603077
affinity capturescdc22ribonucleoside reductase large subunit Cdc22 Affinity Capture-MSPMID:20603077
affinity capturespan1actin cortical patch component, with EF hand and WH2 motif Panl (predicted) Affinity Capture-MSPMID:20603077
affinity capturesccr4CCR4-Not complex subunit Ccr4 (predicted) Affinity Capture-MSPMID:20603077
affinity capturesbbc1WIP family cytoskeletal protein Bbc1 (predicted) Affinity Capture-MSPMID:20603077
affinity capturesnog2ribosome export GTPase Nog2 (predicted) Affinity Capture-MSPMID:20603077
affinity capturesrps260240S ribosomal protein S26 (predicted) Affinity Capture-MSPMID:20603077
affinity capturesmss116mitochondrial ATP-dependent RNA helicase Mss116 (predicted) Affinity Capture-MSPMID:20603077
affinity capturesusp107U1 snRNP-associated protein Usp107 Affinity Capture-MSPMID:20603077
binds DNA-binding domain construct withgef1Cdc42 RhoGEF Gef1 Two-hybridPMID:26771498
affinity captured bycdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Affinity Capture-WesternPMID:20603077
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
modified bylkh1dual specificity protein kinase Lkh1 Biochemical ActivityPMID:26167880
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC9G1.06c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
phenotype enhanced bycdc12formin Cdc12 Phenotypic EnhancementPMID:22573890
phenotype enhanced bycdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Phenotypic EnhancementPMID:22573890
phenotype enhanced bymyo2myosin II heavy chain Phenotypic EnhancementPMID:22573890
phenotype enhanced bycdc4myosin II light chain Phenotypic EnhancementPMID:22573890
negative genetic interaction withmid1medial ring protein Mid1 Negative GeneticPMID:22681890
negative genetic interaction withSPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:22681890
negative genetic interaction withmyo51myosin type V Negative GeneticPMID:22681890
negative genetic interaction withpxl1paxillin-like protein Pxl1 Negative GeneticPMID:25428987
negative genetic interaction withimp2contractile ring protein Imp2 Negative GeneticPMID:25428987
synthetic growth defect withcdc4myosin II light chain Synthetic Growth DefectPMID:22573890
synthetic growth defect withfor3formin For3 Synthetic Growth DefectPMID:18931302
synthetic growth defect withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Synthetic Growth DefectPMID:18931302
positive genetic interaction withmbx1MADS-box transcription factor Mbx1 Positive GeneticPMID:22681890
positive genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
positive genetic interaction withhip4histone promoter control protein Hip4 Positive GeneticPMID:22681890
rescued bychs2chitin synthase homolog Chs2 Phenotypic SuppressionPMID:22573890
rescued byspa2cell polarity protein Spa2 Phenotypic SuppressionPMID:25428987
synthetic lethal withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:25428987
External References
Database Identifier Description
NBRP SPAC9G1.06c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC9G1.06c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC9G1.06c BioGRID Interaction Datasets
Expression Viewer SPAC9G1.06c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC9G1.06c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC9G1.06c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC9G1.06c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC9G1.06c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC9G1.06c Transcriptome Viewer (Bähler Lab)
GEO SPAC9G1.06c GEO profiles
PInt SPAC9G1.06c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC9G1.06c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC9G1.06c Fission yeast phenotypic data & analysis
Cyclebase SPAC9G1.06c.1 Cell Cycle Data
SPD / RIKEN47/47B08Orfeome Localization Data
UniProtKB/SwissProtO14302cytokinesis protein 3
ModBaseO14302Database of comparative protein structure models
STRINGO14302Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593561cytokinesis protein Cyk3 (predicted)
RefSeq mRNANM_001018994972h- cytokinesis protein Cyk3 (predicted) (cyk3), mRNA
European Nucleotide ArchiveCAB11490.1ENA Protein Mapping
UniParcUPI000013AB5CUniProt Archive

Literature for cyk3

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016