cyk3 (SPAC9G1.06c)

Gene Standard Namecyk3 Characterisation Statusbiological_role_inferred
Systematic IDSPAC9G1.06c Feature Typeprotein coding
Synonyms Name Description
Productcytokinesis protein Cyk3 (predicted) Product Size886aa, 98.26 kDa
Genomic Location Chromosome I, 1982206-1976754 (5453nt); CDS:1982107-1979364 (2744nt)

Ensembl Gene Location
GO Biological Process

GO Slim Terms


Term NameCount
mitotic cytokinesis132
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actomyosin contractile ring30
Annotation ExtensionEvidenceWith/FromReference
cell division site301
Annotation ExtensionEvidenceWith/FromReference
cell tip190
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopycyk3ΔNullPMID:204732893759
Microscopycyk3ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001510viable vegetative cell, abnormal cell shape, normal cell size215
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000001
Microscopycyk3ΔNullPECO:0000137, PECO:0000005PMID:23697806
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00018 Pfam IPR001452 Src homology-3 domain 12 59 18
SM00460 SMART IPR002931 Transglutaminase-like 528 595 2
SM00326 SMART IPR001452 Src homology-3 domain 9 66 21
PS50002 Prosite Profiles IPR001452 Src homology-3 domain 6 67 21
PTHR10661:SF104 HMMPANTHER 1 98 1 Gene3D 4 63 21
SSF54001 SuperFamily 494 637 30
SSF50044 SuperFamily IPR001452 Src homology-3 domain 5 87 21

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.91 Da
Charge 31.50
Isoelectric point 9.75
Molecular weight 98.26 kDa
Number of residues 886

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS390PMID:247631071670
experimental evidenceS209PMID:24763107
present during mitotic M phaseexperimental evidenceS341PMID:21712547
experimental evidenceS213PMID:24763107
present during mitotic M phaseexperimental evidenceS390PMID:21712547
present during mitotic M phaseexperimental evidenceS131PMID:21712547
experimental evidenceS143PMID:24763107
level fluctuates during mitotic cell cycleexperimental evidenceS267PMID:24763107
present during mitotic M phaseexperimental evidenceS137PMID:21712547
present during mitotic M phaseexperimental evidenceS141PMID:21712547
present during mitotic M phaseexperimental evidenceS337PMID:21712547
present during mitotic M phaseexperimental evidenceS267PMID:21712547
present during mitotic M phaseexperimental evidenceS140PMID:21712547
present during mitotic M phaseexperimental evidenceS207PMID:21712547
present during mitotic M phaseexperimental evidenceS125PMID:21712547
present during mitotic M phaseexperimental evidenceS123PMID:21712547
present during mitotic M phaseexperimental evidenceS143PMID:21712547
present during mitotic M phaseexperimental evidenceS213PMID:21712547
present during mitotic M phaseexperimental evidenceS209PMID:21712547
present during mitotic M phaseexperimental evidenceS261PMID:21712547
MOD:00047O-phospho-L-threonineexperimental evidenceT219PMID:21712547692
present during mitotic M phase
present during mitotic M phaseexperimental evidenceT332PMID:21712547
present during mitotic M phaseexperimental evidenceT393PMID:21712547
present during mitotic M phaseexperimental evidenceT260PMID:21712547
present during mitotic M phaseexperimental evidenceT338PMID:21712547
experimental evidenceT332PMID:24763107
MOD:01455O-phosphorylated residueIDAS267PMID:232973481749
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1871during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
2237during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
1961during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
2333during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
3103.53during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
1264during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
4039.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.1during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.84during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi only623
conserved in fungi4600
conserved in eukaryotes4514

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
SPBC691.04mitochondrial ATP-dependent RNA helicase Mss116 (predicted) Affinity Capture-MSPMID:20603077
rad2414-3-3 protein Rad24 Affinity Capture-MSPMID:20603077
myo1myosin type I Affinity Capture-MSPMID:20603077
mug64BAR domain protein (predicted) Affinity Capture-MSPMID:20603077
gpd1glycerol-3-phosphate dehydrogenase Gpd1 Affinity Capture-MSPMID:20603077
cdc22ribonucleoside reductase large subunit Cdc22 Affinity Capture-MSPMID:20603077
SPAC23A1.17WIP family cytoskeletal protein (predicted) Affinity Capture-MSPMID:20603077
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
rps260240S ribosomal protein S26 (predicted) Affinity Capture-MSPMID:20603077
SPAC821.05translation initiation factor eIF3h (p40) Affinity Capture-MSPMID:20603077
SPAC24C9.06caconitate hydratase (predicted) Affinity Capture-MSPMID:20603077
rad2514-3-3 protein Rad25 Affinity Capture-MSPMID:20603077
SPAC1751.03translation initiation factor eIF3m Affinity Capture-MSPMID:20603077
pan1actin cortical patch component, with EF hand and WH2 motif Panl (predicted) Affinity Capture-MSPMID:20603077
ccr4CCR4-Not complex subunit Ccr4 (predicted) Affinity Capture-MSPMID:20603077
shm2serine hydroxymethyltransferase Shm2 (predicted) Affinity Capture-MSPMID:20603077
SPAC6F6.03cribosome export GTPase (predicted) Affinity Capture-MSPMID:20603077
ala1mitochondrial and cytoplasmic alanine-tRNA ligase Ala1 (predicted) Affinity Capture-MSPMID:20603077
hhf1histone H4 h4.1 Affinity Capture-MSPMID:20603077
fic1C2 domain protein Fic1 Affinity Capture-WesternPMID:23093943
apl2AP-1 adaptor complex subunit beta subunit Apl2 Affinity Capture-MSPMID:20603077
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Affinity Capture-WesternPMID:20603077
Affinity Capture-MS
sec21coatomer gamma subunit Sec21 (predicted) Affinity Capture-MSPMID:20603077
usp107U1 snRNP-associated protein Usp107 Affinity Capture-MSPMID:20603077
nrs1cytoplasmic asparagine-tRNA ligase Nrs1 (predicted) Affinity Capture-MSPMID:20603077
nog1GTP binding protein Nog1 (predicted) Affinity Capture-MSPMID:20603077
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
SPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:22681890
chs2chitin synthase homolog Chs2 Phenotypic SuppressionPMID:22573890
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Phenotypic EnhancementPMID:22573890
myo2myosin II heavy chain Phenotypic EnhancementPMID:22573890
mbx1MADS-box transcription factor Mbx1 Positive GeneticPMID:22681890
for3formin For3 Synthetic Growth DefectPMID:18931302
myo51myosin type V Negative GeneticPMID:22681890
cdc12formin Cdc12 Phenotypic EnhancementPMID:22573890
hip4histone promoter control protein Hip4 Positive GeneticPMID:22681890
SPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
mid1medial ring protein Mid1 Negative GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Synthetic Growth DefectPMID:18931302
cdc4myosin II light chain Synthetic Growth DefectPMID:22573890
Phenotypic Enhancement
External References
Database Identifier Description
NBRP SPAC9G1.06c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC9G1.06c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC9G1.06c BioGRID Interaction Datasets
Expression Viewer SPAC9G1.06c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC9G1.06c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC9G1.06c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC9G1.06c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC9G1.06c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC9G1.06c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC9G1.06c Cell Cycle Data
GEO SPAC9G1.06c GEO profiles
PInt SPAC9G1.06c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC9G1.06c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC9G1.06c Fission yeast phenotypic data & analysis
SPD / RIKEN47/47B08Orfeome Localization Data
UniProtKB/SwissProtO14302cytokinesis protein 3
ModBaseO14302Database of comparative protein structure models
STRINGO14302Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593561cytokinesis protein Cyk3 (predicted)
RefSeq mRNANM_001018994972h- cytokinesis protein Cyk3 (predicted) (cyk3), mRNA
European Nucleotide ArchiveCAB11490.1ENA Protein Mapping
UniParcUPI000013AB5CUniProt Archive

Literature for cyk3

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014