mpp6 (SPACUNK4.11c)


Gene Standard Namempp6 Characterisation Statusbiological role inferred
Systematic IDSPACUNK4.11c Feature Typeprotein coding
Synonyms Name Description
Productnuclear exosome-associated RNA binding protein Mpp6 Product Size188aa, 21.51 kDa
Genomic Location Chromosome I, 2890095-2890887 (793nt); CDS:2890158-2890779 (622nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
nuclear mRNA surveillance4
Annotation ExtensionEvidenceWith/FromReference
polyadenylation-dependent RNA catabolic process18
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nuclear exosome (RNase complex)13
Annotation ExtensionEvidenceWith/FromReference
nucleolus365
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
sensitive to 6-azauracilmpp6Δ70
viable vegetative cell populationmpp6Δ3840

Cell Phenotype

Term NameGenotypesCount
normal meiosismpp6Δ149
viable vegetative cell with normal cell morphologympp6Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2890095..2890184, 2890240..2890887
Intron2890185..2890239
mRNA2890095..2890887
5' UTR2890095..2890157PMID:21511999
CDS2890158..2890184, 2890240..2890779
3' UTR2890780..2890887AU010352
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF10175 Pfam IPR019324 M-phase phosphoprotein 6 2 139 1
PTHR13582 HMMPANTHER IPR019324 M-phase phosphoprotein 6 1 188 1
Coil ncoils Predicted coiled-coil protein (DUF2205) 120 140 1057
Coil ncoils Predicted coiled-coil protein (DUF2205) 173 188 1057

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.39 Da
Charge 7.50
Codon Adaptation Index 0.39
Isoelectric point 10.02
Molecular weight 21.51 kDa
Number of residues 188
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS167
level fluctuates during mitotic cell cycleS167
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S167 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence S167 PMID:24763107
phosphorylated residue 1931
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for mpp6 (SPACUNK4.11c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8102during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
12781during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
9043during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
12649during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8991during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2098.59during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.51during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2506
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPACUNK4.11c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
affinity captured byrrp43exosome subunit Rrp43 Affinity Capture-MSPMID:23503588
affinity captured byred1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
affinity captured byiss10Mmi1-driven selective elimination factor Iss10 Affinity Capture-MSPMID:24713849
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPACUNK4.11c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withSPBPB10D8.02carylsulfatase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withurk1uridine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withubc6ubiquitin conjugating enzyme E2 Ubc6 (predicted) Positive GeneticPMID:22681890
positive genetic interaction witheif21translation initiation factor eIF2A (predicted) Positive GeneticPMID:22681890
positive genetic interaction withpby1tubulin-tyrosine ligase Pby1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsgf29SAGA complex subunit Sgf29 Positive GeneticPMID:22681890
positive genetic interaction withcpd1tRNA (m1A) methyltransferase complex catalytic subunit Cpd1 Positive GeneticPMID:22681890
positive genetic interaction withphp5CCAAT-binding factor complex subunit Php5 Positive GeneticPMID:22681890
negative genetic interaction witharp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:22681890
negative genetic interaction withair1zinc knuckle TRAMP complex subunit Air1 Negative GeneticPMID:22681890
negative genetic interaction withplb1phospholipase B homolog Plb1 Negative GeneticPMID:22681890
negative genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction withsrb11cyclin CycC, Srb mediator subunit Srb11 Negative GeneticPMID:22681890
negative genetic interaction withrsc4RSC complex subunit Rsc4 Negative GeneticPMID:22681890
negative genetic interaction withpst2Clr6 histone deacetylase complex subunit Pst2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPACUNK4.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPACUNK4.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPACUNK4.11c BioGRID Interaction Datasets
Expression Viewer SPACUNK4.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPACUNK4.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPACUNK4.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPACUNK4.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPACUNK4.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPACUNK4.11c Transcriptome Viewer (Bähler Lab)
GEO SPACUNK4.11c GEO profiles
PInt SPACUNK4.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPACUNK4.11c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPACUNK4.11c Fission yeast phenotypic data & analysis
Cyclebase SPACUNK4.11c.1 Cell Cycle Data
SPD / RIKEN07/07A07Orfeome Localization Data
UniProtKB/SwissProtO14076Uncharacterized protein UNK4.11c
ModBaseO14076Database of comparative protein structure models
STRINGO14076Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593967M-phase phosphoprotein 6 family protein
RefSeq mRNANM_001019394972h- M-phase phosphoprotein 6 family protein (SPACUNK4.11c), mRNA

Literature for mpp6

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016