cdc4 (SPAP8A3.08)


Gene Standard Namecdc4 Characterisation Statuspublished
Systematic IDSPAP8A3.08 Feature Typeprotein coding
Synonyms Name Description
Productmyosin II light chain Product Size141aa, 15.66 kDa
Genomic Location Chromosome I, 5329177-5331057 (1881nt); CDS:5329218-5329906 (689nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
metal ion binding743
Annotation ExtensionEvidenceWith/FromReference
protein binding859
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitotic actomyosin contractile ring assembly27
Annotation ExtensionEvidenceWith/FromReference
mitotic actomyosin contractile ring assembly actin filament organization6
Annotation ExtensionEvidenceWith/FromReference
protein localization to medial cortex6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actomyosin contractile ring38
Annotation ExtensionEvidenceWith/FromReference
cell division site305
Annotation ExtensionEvidenceWith/FromReference
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
medial cortical node10
Annotation ExtensionEvidenceWith/FromReference
myosin II complex4
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationcdc4-A1Not specified1438
cdc4-A11Not specified
cdc4-A14Not specified
cdc4-A2Not specified
cdc4-A4Not specified
cdc4-A9Not specified
cdc4-B4Not specified
cdc4-C2Not specified
cdc4-D1Not specified
cdc4-D2Not specified
cdc4-D3Not specified
cdc4-E2Not specified
cdc4-E4Not specified
cdc4ΔNull
unnamed (unrecorded)
viable vegetative cell populationcdc4-8Not specified3809
cdc4-A1Not specified
cdc4-A11Not specified
cdc4-A14Not specified
cdc4-A2Not specified
cdc4-A4Not specified
cdc4-A9Not specified
cdc4-B4Not specified
cdc4-C2Not specified
cdc4-D1Not specified
cdc4-D2Not specified
cdc4-D3Not specified
cdc4-E2Not specified
cdc4-E4Not specified

Cell Phenotype

Term NameAlleleExpressionCount
abnormal actin cable organization during mitosiscdc4Not specified2
abnormal actomyosin contractile ring assemblycdc4-3115
cdc4-8
cdc4-D3Not specified
abnormal protein localization to medial cortex during vegetative growth25
affecting cdc15cdc4ΔNull
affecting rng2cdc4ΔNull
affecting rlc1cdc4ΔNull
abnormal protein localization to medial cortical node4
affecting cdc12cdc4-377Not specified
abnormal septationcdc4-31151
cdc4-8
abolished protein localization to actomyosin contractile ring11
affecting chs2cdc4-8Not specified
affecting pxl1cdc4-8Not specified
abolished protein localization to medial cortex during vegetative growth18
affecting pxl1cdc4-8Not specified
abolished septum formationcdc4-377Not specified64
actomyosin contractile ring absentcdc4-377Not specified9
elongated multinucleate multiseptate vegetative cell, septa between nucleicdc4-8Not specified17
elongated multinucleate vegetative cellcdc4-839
inviable elongated multinucleate vegetative cellcdc4-377Not specified13
inviable elongated multinucleate vegetative cell with central constrictioncdc4-31Endogenous4
cdc4-8Endogenous
cdc4-8Not specified
inviable elongated vegetative cell with central constrictioncdc4+ (wild type)Knockdown5
spore lysiscdc4ΔNull3
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in chs2 chitin synthase homolog Chs2
FYPO affected by mutation in rng2 IQGAP
GO localized by rng2 IQGAP
GO regulated by ace2 transcription factor Ace2
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons5329177..5329248, 5329301..5329432, 5329506..5329646, 5329713..5329805, 5329878..5331057
mRNA5329177..5331057
5' UTR5329177..5329217PMID:21511999
CDS5329218..5329248, 5329301..5329432, 5329506..5329646, 5329713..5329805, 5329878..5329906
3' UTR5329907..5331057PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13405 Pfam IPR002048 EF-hand domain 9 34 4
PF13499 Pfam IPR011992 EF-hand domain pair 83 138 7
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 87 99 12
PS50222 Prosite Profiles IPR002048 EF-hand domain 125 141 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 74 109 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 3 38 20
PTHR23048 HMMPANTHER 8 141 1
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 8 81 20
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 82 138 20
SSF47473 SuperFamily 7 138 21

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276

Protein Properties

Ave. residue weight 111.07 Da
Charge -11.00
Isoelectric point 4.16
Molecular weight 15.66 kDa
Number of residues 141
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
122236during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
114591during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
116485during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
110981during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
77000during GO:0072690PECO:0000005,
PECO:0000012
single cellexperimental evidencePMID:16224022
16916.2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
98134during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
19695.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7.8during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
affinity capturesmid1medial ring protein Mid1 Affinity Capture-WesternPMID:21422229
affinity capturesmid1medial ring protein Mid1 Affinity Capture-WesternPMID:22427686
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:10769212
affinity captured bymyo2myosin II heavy chain Affinity Capture-WesternPMID:10769212
binds activation domain construct withmyo2myosin II heavy chain Two-hybridPMID:10769212
affinity captured bymyo2myosin II heavy chain Affinity Capture-WesternPMID:15504913
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:11087749
affinity captured bymyo2myosin II heavy chain Affinity Capture-WesternPMID:11056543
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:10022828
affinity captured bymyo2myosin II heavy chain Affinity Capture-WesternPMID:10022828
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:21422229
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:11942609
forms complex withmyo2myosin II heavy chain Reconstituted ComplexPMID:21693583
binds activation domain construct withmyp2myosin II heavy chain Myo3 Two-hybridPMID:10769212
affinity capturesmyp2myosin II heavy chain Myo3 Affinity Capture-WesternPMID:10769212
affinity captured bymyp2myosin II heavy chain Myo3 Affinity Capture-WesternPMID:10769212
forms complex withpik11-phosphatidylinositol 4-kinase Pik1 Reconstituted ComplexPMID:11087749
forms complex withpik11-phosphatidylinositol 4-kinase Pik1 Reconstituted ComplexPMID:21693583
affinity captured byrlc1myosin II regulatory light chain Rlc1 Affinity Capture-WesternPMID:11069761
affinity captured byrlc1myosin II regulatory light chain Rlc1 Affinity Capture-WesternPMID:11056543
binds DNA-binding domain construct withrng2IQGAP Two-hybridPMID:21422229
affinity captured byrng2IQGAP Affinity Capture-MSPMID:20603077
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withacp1F-actin capping protein alpha subunit Synthetic LethalityPMID:16866873
enhances phenotype ofacp2F-actin capping protein beta subunit Acp2 Phenotypic EnhancementPMID:16866873
synthetic lethal withacp2F-actin capping protein beta subunit Acp2 Synthetic LethalityPMID:16866873
synthetic growth defect withacp2F-actin capping protein beta subunit Acp2 Synthetic Growth DefectPMID:15743909
synthetic lethal withact1actin Act1 Synthetic LethalityPMID:10371213
synthetically rescued byact1actin Act1 Synthetic RescuePMID:10371213
synthetic growth defect withact1actin Act1 Synthetic Growth DefectPMID:23589458
synthetic lethal withact1actin Act1 Synthetic LethalityPMID:23589458
enhances phenotype ofadf1actin depolymerizing factor, cofilin Phenotypic EnhancementPMID:16467379
synthetic lethal withadf1actin depolymerizing factor, cofilin Synthetic LethalityPMID:16467379
synthetic lethal withase1antiparallel microtubule cross-linking factor Ase1 Synthetic LethalityPMID:15647375
enhances phenotype ofbgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:10503548
synthetic growth defect withblt1ubiquitin domain-like protein Blt1 Synthetic Growth DefectPMID:24790095
synthetically rescued bycam2myosin I light chain Cam2 Synthetic RescuePMID:21693583
rescued bycam2myosin I light chain Cam2 Phenotypic SuppressionPMID:21693583
synthetic lethal withcdc12formin Cdc12 Synthetic LethalityPMID:24127216
synthetic lethal withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:7634333
synthetic lethal withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:19139265
synthetic lethal withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:20603077
synthetic lethal withchs2chitin synthase homolog Chs2 Synthetic LethalityPMID:16772338
synthetic growth defect withclp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic Growth DefectPMID:15265986
synthetic growth defect withcyk3cytokinesis protein Cyk3 (predicted) Synthetic Growth DefectPMID:22573890
enhances phenotype ofcyk3cytokinesis protein Cyk3 (predicted) Phenotypic EnhancementPMID:22573890
synthetic lethal withfor3formin For3 Synthetic LethalityPMID:24127216
phenotype enhanced bygef2RhoGEF Gef2 Phenotypic EnhancementPMID:23966468
rescued byhsp9heat shock protein Hsp9 Phenotypic SuppressionPMID:8654972
synthetic growth defect withimp2contractile ring protein Imp2 Synthetic Growth DefectPMID:19139265
synthetic growth defect withimp2contractile ring protein Imp2 Synthetic Growth DefectPMID:9786952
growth defect in presence of overexpressedimp2contractile ring protein Imp2 Dosage Growth DefectPMID:9786952
rescued bykin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:23294323
synthetic growth defect withmob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Synthetic Growth DefectPMID:10769201
synthetically rescued bymyo2myosin II heavy chain Synthetic RescuePMID:10769212
synthetic lethal withmyo2myosin II heavy chain Synthetic LethalityPMID:10022828
synthetic lethal withmyp2myosin II heavy chain Myo3 Synthetic LethalityPMID:20739711
phenotype enhanced bynod1medial cortical node Gef2-related protein protein Nod1 Phenotypic EnhancementPMID:23966468
synthetic lethal withpxl1paxillin-like protein Pxl1 Synthetic LethalityPMID:18256290
synthetic lethal withrng2IQGAP Synthetic LethalityPMID:9635188
synthetically rescued byrng2IQGAP Synthetic RescuePMID:23615450
synthetic growth defect withscw1RNA-binding protein Scw1 Synthetic Growth DefectPMID:20739711
enhances phenotype ofsep1forkhead transcription factor Sep1 Phenotypic EnhancementPMID:8505375
synthetic lethal withspn1mitotic septin Spn1 Synthetic LethalityPMID:20739711
synthetic growth defect withspn4mitotic septin Spn4 Synthetic Growth DefectPMID:20739711
External References
Database Identifier Description
NBRP SPAP8A3.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAP8A3.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAP8A3.08 BioGRID Interaction Datasets
Expression Viewer SPAP8A3.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAP8A3.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAP8A3.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAP8A3.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAP8A3.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAP8A3.08 Transcriptome Viewer (Bähler Lab)
GEO SPAP8A3.08 GEO profiles
PInt SPAP8A3.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAP8A3.08 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAP8A3.08 Fission yeast phenotypic data & analysis
Cyclebase SPAP8A3.08.1 Cell Cycle Data
UniProtKB/SwissProtQ09196Myosin regulatory light chain cdc4
ModBaseQ09196Database of comparative protein structure models
STRINGQ09196Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594947myosin II light chain
RefSeq mRNANM_001020378972h- myosin II light chain (cdc4), mRNA
ePDB1GGWThe European PDB
PDB1GGWPDB
PDBsum1GGWPDBsum
European Nucleotide ArchiveAAA67467.1ENA Protein Mapping
European Nucleotide ArchiveCAB55175.1ENA Protein Mapping
UniParcUPI000012F20EUniProt Archive

Literature for cdc4

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Release Version: PomBase:26_52 - 11 May 2015