cdc4 (SPAP8A3.08)


Gene Standard Namecdc4 Characterisation Statuspublished
Systematic IDSPAP8A3.08 Feature Typeprotein coding
Synonyms Name Description
Productmyosin II light chain Product Size141aa, 15.66 kDa
Genomic Location Chromosome I, 5329177-5331057 (1881nt); CDS:5329218-5329906 (689nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding872
Annotation ExtensionEvidenceWith/FromReference
NOT calcium ion binding
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitotic actomyosin contractile ring assembly28
Annotation ExtensionEvidenceWith/FromReference
mitotic actomyosin contractile ring assembly actin filament organization7
Annotation ExtensionEvidenceWith/FromReference
protein localization to medial cortex6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actomyosin contractile ring39
Annotation ExtensionEvidenceWith/FromReference
cell division site313
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
medial cortical node11
Annotation ExtensionEvidenceWith/FromReference
myosin II complex4
Annotation ExtensionEvidenceWith/FromReference
nucleus2691
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationcdc4Δ1452
cdc4
viable vegetative cell populationcdc43844

Cell Phenotype

Term NameGenotypesCount
abnormal actin cable organization during mitosiscdc42
abnormal actomyosin contractile ring assemblycdc416
cdc4
abnormal protein localization to medial cortex during vegetative growth29
affects localization of cdc15cdc4Δ
affects localization of rng2cdc4Δ
affects localization of rlc1cdc4Δ
abnormal protein localization to medial cortical node4
affects localization of cdc12cdc4
abnormal septationcdc4293
abolished protein localization to actomyosin contractile ring12
affects localization of chs2cdc4
affects localization of pxl1cdc4
abolished protein localization to medial cortex during vegetative growth21
affects localization of pxl1cdc4
abolished septum formationcdc489
actomyosin contractile ring absentcdc49
elongated multinucleate vegetative cellcdc447
elongated multiseptate vegetative cell, with three or more nuclei and single septa between nucleicdc417
inviable elongated multinucleate vegetative cellcdc418
inviable elongated multinucleate vegetative cell with central constrictioncdc44
inviable elongated vegetative cell with central constrictioncdc4+5
spore lysiscdc4Δ4

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
inviable vegetative cell populationchs2Δ, cdc4
pxl1Δ, cdc4

Cell Phenotype

Term NameGenotypes
inviable branched, elongated, multiseptate vegetative cellpxl1Δ, cdc4
Target Of
OntologyRelationshipGeneProduct
FYPO localization affected by mutation in chs2 chitin synthase homolog Chs2
FYPO localization affected by mutation in rng2 IQGAP
GO localized by rng2 IQGAP
GO regulated by ace2 transcription factor Ace2
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons5329177..5329248, 5329301..5329432, 5329506..5329646, 5329713..5329805, 5329878..5331057
Intron5329249..5329300, 5329433..5329505, 5329647..5329712, 5329806..5329877
mRNA5329177..5331057
5' UTR5329177..5329217PMID:21511999
CDS5329218..5329248, 5329301..5329432, 5329506..5329646, 5329713..5329805, 5329878..5329906
3' UTR5329907..5331057PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13499 Pfam IPR002048 EF-hand domain 83 138 8
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 87 99 12
PS50222 Prosite Profiles IPR002048 EF-hand domain 3 38 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 74 109 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 125 141 20
PTHR23048 HMMPANTHER 8 141 1
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 82 138 20
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 8 81 20
SSF47473 SuperFamily IPR011992 EF-hand domain pair 7 138 21

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276

Protein Properties

Ave. residue weight 111.07 Da
Charge -11.00
Codon Adaptation Index 0.49
Isoelectric point 4.16
Molecular weight 15.66 kDa
Number of residues 141
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for cdc4 (SPAP8A3.08)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
122236during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
114591during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
116485during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
110981during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
16916.2during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
98134during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
77000during GO:0072690PECO:0000012,
PECO:0000005
single cellexperimental evidencePMID:16224022
19695.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7.8during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
monomeric PMID:1108775012
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAP8A3.08 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withmyp2myosin II heavy chain Myo3 Two-hybridPMID:10769212
binds activation domain construct withsnd1RNA-binding protein, involved in chromatin silencing by small RNA, Snd1 Two-hybridPMID:26771498
binds activation domain construct withmyo2myosin II heavy chain Two-hybridPMID:10769212
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:11087749
affinity capturesmyp2myosin II heavy chain Myo3 Affinity Capture-WesternPMID:10769212
affinity capturesmid1medial ring protein Mid1 Affinity Capture-WesternPMID:22427686
affinity captured bymyo2myosin II heavy chain Affinity Capture-WesternPMID:11056543
affinity captured byrlc1myosin II regulatory light chain Rlc1 Affinity Capture-WesternPMID:11069761
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
affinity captured bymyp2myosin II heavy chain Myo3 Affinity Capture-WesternPMID:10769212
affinity captured byrng2IQGAP Affinity Capture-MSPMID:20603077
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
forms complex withpik11-phosphatidylinositol 4-kinase Pik1 Reconstituted ComplexPMID:11087749
forms complex withmyo2myosin II heavy chain Reconstituted ComplexPMID:21693583
binds DNA-binding domain construct withrng2IQGAP Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAP8A3.08 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
enhances phenotype ofbgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:10503548
enhances phenotype ofsep1forkhead transcription factor Sep1 Phenotypic EnhancementPMID:8505375
enhances phenotype ofacp2F-actin capping protein beta subunit Acp2 Phenotypic EnhancementPMID:16866873
enhances phenotype ofcyk3cytokinesis protein Cyk3 (predicted) Phenotypic EnhancementPMID:22573890
enhances phenotype ofadf1actin depolymerizing factor, cofilin Phenotypic EnhancementPMID:16467379
synthetic lethal withmyo2myosin II heavy chain Synthetic LethalityPMID:10022828
synthetic lethal withspn1mitotic septin Spn1 Synthetic LethalityPMID:20739711
synthetic lethal withacp1F-actin capping protein alpha subunit Synthetic LethalityPMID:16866873
synthetic lethal withact1actin Act1 Synthetic LethalityPMID:10371213
synthetic lethal withacp2F-actin capping protein beta subunit Acp2 Synthetic LethalityPMID:16866873
synthetic lethal withadf1actin depolymerizing factor, cofilin Synthetic LethalityPMID:16467379
synthetic lethal withfor3formin For3 Synthetic LethalityPMID:24127216
synthetic lethal withrng2IQGAP Synthetic LethalityPMID:9635188
synthetic lethal withase1antiparallel microtubule cross-linking factor Ase1 Synthetic LethalityPMID:15647375
synthetic lethal withpxl1paxillin-like protein Pxl1 Synthetic LethalityPMID:18256290
synthetic lethal withmyp2myosin II heavy chain Myo3 Synthetic LethalityPMID:20739711
synthetic lethal withcdc12formin Cdc12 Synthetic LethalityPMID:24127216
synthetic lethal withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:20603077
synthetic lethal withchs2chitin synthase homolog Chs2 Synthetic LethalityPMID:16772338
synthetically rescued byrng2IQGAP Synthetic RescuePMID:23615450
synthetically rescued byact1actin Act1 Synthetic RescuePMID:10371213
synthetically rescued bymyo2myosin II heavy chain Synthetic RescuePMID:10769212
synthetically rescued bycam2myosin I light chain Cam2 Synthetic RescuePMID:21693583
synthetic growth defect withblt1ubiquitin domain-like protein Blt1 Synthetic Growth DefectPMID:24790095
synthetic growth defect withscw1RNA-binding protein Scw1 Synthetic Growth DefectPMID:20739711
synthetic growth defect withimp2contractile ring protein Imp2 Synthetic Growth DefectPMID:19139265
synthetic growth defect withacp2F-actin capping protein beta subunit Acp2 Synthetic Growth DefectPMID:15743909
synthetic growth defect withact1actin Act1 Synthetic Growth DefectPMID:23589458
synthetic growth defect withmob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Synthetic Growth DefectPMID:10769201
synthetic growth defect withclp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic Growth DefectPMID:15265986
synthetic growth defect withspn4mitotic septin Spn4 Synthetic Growth DefectPMID:20739711
synthetic growth defect withcyk3cytokinesis protein Cyk3 (predicted) Synthetic Growth DefectPMID:22573890
rescued bykin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:23294323
rescued byhsp9heat shock protein Hsp9 Phenotypic SuppressionPMID:8654972
rescued bycam2myosin I light chain Cam2 Phenotypic SuppressionPMID:21693583
growth defect in presence of overexpressedimp2contractile ring protein Imp2 Dosage Growth DefectPMID:9786952
phenotype enhanced bygef2RhoGEF Gef2 Phenotypic EnhancementPMID:23966468
phenotype enhanced bynod1medial cortical node Gef2-related protein protein Nod1 Phenotypic EnhancementPMID:23966468
External References
Database Identifier Description
NBRP SPAP8A3.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAP8A3.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAP8A3.08 BioGRID Interaction Datasets
Expression Viewer SPAP8A3.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAP8A3.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAP8A3.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAP8A3.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAP8A3.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAP8A3.08 Transcriptome Viewer (Bähler Lab)
GEO SPAP8A3.08 GEO profiles
PInt SPAP8A3.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAP8A3.08 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAP8A3.08 Fission yeast phenotypic data & analysis
Cyclebase SPAP8A3.08.1 Cell Cycle Data
UniProtKB/SwissProtQ09196Myosin regulatory light chain cdc4
ModBaseQ09196Database of comparative protein structure models
STRINGQ09196Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594947myosin II light chain
RefSeq mRNANM_001020378972h- myosin II light chain (cdc4), mRNA
ePDB1GGWThe European PDB
PDB1GGWPDB
PDBsum1GGWPDBsum
European Nucleotide ArchiveAAA67467.1ENA Protein Mapping
European Nucleotide ArchiveCAB55175.1ENA Protein Mapping
UniParcUPI000012F20EUniProt Archive

Literature for cdc4

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016