cdc4 (SPAP8A3.08)


Gene Standard Namecdc4 Characterisation Statuspublished
Systematic IDSPAP8A3.08 Feature Typeprotein coding
Synonyms Name Description
Productmyosin II light chain Product Size141aa, 15.66 kDa
Genomic Location Chromosome I, 5329177-5331057 (1881nt); CDS:5329218-5329906 (689nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
metal ion binding746
Annotation ExtensionEvidenceWith/FromReference
protein binding851
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitotic actomyosin contractile ring assembly28
Annotation ExtensionEvidenceWith/FromReference
mitotic actomyosin contractile ring assembly actin filament organization6
Annotation ExtensionEvidenceWith/FromReference
protein localization to medial cortex6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actomyosin contractile ring33
Annotation ExtensionEvidenceWith/FromReference
cell division site300
Annotation ExtensionEvidenceWith/FromReference
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
medial cortical node10
Annotation ExtensionEvidenceWith/FromReference
myosin II complex4
Annotation ExtensionEvidenceWith/FromReference
nucleus2697
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationcdc4ΔNull1428
unnamed (unrecorded)

Cell Phenotype

Term NameAlleleExpressionCount
abnormal actin cable organization during mitosiscdc4Not specified2
abnormal actomyosin contractile ring assemblycdc4-3168
cdc4-8
abnormal protein localization to medial cortex during vegetative growth17
affecting cdc15cdc4ΔNull
affecting rng2cdc4ΔNull
affecting rlc1cdc4ΔNull
abnormal protein localization to medial cortical node3
affecting cdc12cdc4-377Not specified
abnormal septationcdc4-31121
cdc4-8
abolished protein localization to actomyosin contractile ring11
affecting chs2cdc4-8Not specified
affecting pxl1cdc4-8Not specified
abolished protein localization to medial cortex during vegetative growth10
affecting pxl1cdc4-8Not specified
abolished septum formationcdc4-377Not specified60
actomyosin contractile ring absentcdc4-377Not specified66
elongated multinucleate multiseptate vegetative cell, septa between nucleicdc4-8Not specified16
elongated multinucleate vegetative cellcdc4-835
increased spontaneous diploidizationcdc4-377Not specified24
inviable elongated dumbbell-shaped cellcdc4ΔNull5
inviable elongated multinucleate vegetative cellcdc4-377Not specified12
inviable, elongated, multinucleate dumbbell-shaped cellcdc4-31Endogenous4
cdc4-8Endogenous
cdc4-8Not specified
spore lysiscdc4ΔNull3
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in chs2 chitin synthase homolog Chs2 PMID:16772338
FYPO affected by mutation in rng2 IQGAP PMID:21376595
GO localized by rng2 IQGAP PMID:21376595
GO regulated by ace2 transcription factor Ace2 PMID:15195092
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
153291775329248
253293015329432
353295065329646
453297135329805
553298785331057

UTRs

Region Coordinates Reference
five_prime_UTR5329177..5329217PMID:21511999
three_prime_UTR5329907..5331057PMID:21511999
mRNA5329177..5331057
exon5329218..5329248,5329301..5329432,5329506..5329646,5329713..5329805,5329878..5329906
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13405 Pfam IPR002048 EF-hand domain 9 34 4
PF13499 Pfam IPR011992 EF-hand domain pair 83 138 7
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 87 99 12
PS50222 Prosite Profiles IPR002048 EF-hand domain 3 38 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 125 141 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 74 109 20
PTHR23048 HMMPANTHER 8 141 1
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 82 138 20
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 8 81 20
SSF47473 SuperFamily 7 138 21

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276

Protein Properties

Ave. residue weight 111.07 Da
Charge -11.00
Isoelectric point 4.16
Molecular weight 15.66 kDa
Number of residues 141
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
122236during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
114591during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
116485during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
110981during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
98134during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
16916.2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
77000during GO:0072690PECO:0000005,
PECO:0000012
single cellexperimental evidencePMID:16224022
19695.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7.8during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
affinity capturesmid1medial ring protein Mid1 Affinity Capture-WesternPMID:21422229
affinity capturesmid1medial ring protein Mid1 Affinity Capture-WesternPMID:22427686
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:10769212
affinity captured bymyo2myosin II heavy chain Affinity Capture-WesternPMID:10769212
binds activation domain construct withmyo2myosin II heavy chain Two-hybridPMID:10769212
affinity captured bymyo2myosin II heavy chain Affinity Capture-WesternPMID:15504913
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:11087749
affinity captured bymyo2myosin II heavy chain Affinity Capture-WesternPMID:11056543
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:10022828
affinity captured bymyo2myosin II heavy chain Affinity Capture-WesternPMID:10022828
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:21422229
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:11942609
forms complex withmyo2myosin II heavy chain Reconstituted ComplexPMID:21693583
binds activation domain construct withmyp2myosin II heavy chain Myo3 Two-hybridPMID:10769212
affinity captured bymyp2myosin II heavy chain Myo3 Affinity Capture-WesternPMID:10769212
affinity capturesmyp2myosin II heavy chain Myo3 Affinity Capture-WesternPMID:10769212
forms complex withpik11-phosphatidylinositol 4-kinase Pik1 Reconstituted ComplexPMID:11087749
forms complex withpik11-phosphatidylinositol 4-kinase Pik1 Reconstituted ComplexPMID:21693583
affinity captured byrlc1myosin II regulatory light chain Rlc1 Affinity Capture-WesternPMID:11069761
affinity captured byrlc1myosin II regulatory light chain Rlc1 Affinity Capture-WesternPMID:11056543
binds DNA-binding domain construct withrng2IQGAP Two-hybridPMID:21422229
affinity captured byrng2IQGAP Affinity Capture-MSPMID:20603077
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withacp1F-actin capping protein alpha subunit Synthetic LethalityPMID:16866873
enhances phenotype ofacp2F-actin capping protein beta subunit Acp2 Phenotypic EnhancementPMID:16866873
synthetic lethal withacp2F-actin capping protein beta subunit Acp2 Synthetic LethalityPMID:16866873
synthetic growth defect withacp2F-actin capping protein beta subunit Acp2 Synthetic Growth DefectPMID:15743909
synthetic lethal withact1actin Act1 Synthetic LethalityPMID:10371213
synthetically rescued byact1actin Act1 Synthetic RescuePMID:10371213
synthetic lethal withact1actin Act1 Synthetic LethalityPMID:23589458
synthetic growth defect withact1actin Act1 Synthetic Growth DefectPMID:23589458
enhances phenotype ofadf1actin depolymerizing factor, cofilin Phenotypic EnhancementPMID:16467379
synthetic lethal withadf1actin depolymerizing factor, cofilin Synthetic LethalityPMID:16467379
synthetic lethal withase1antiparallel microtubule cross-linking factor Ase1 Synthetic LethalityPMID:15647375
enhances phenotype ofbgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:10503548
synthetic growth defect withblt1ubiquitin domain-like protein Blt1 Synthetic Growth DefectPMID:24790095
synthetically rescued bycam2myosin I light chain Cam2 Synthetic RescuePMID:21693583
rescued bycam2myosin I light chain Cam2 Phenotypic SuppressionPMID:21693583
synthetic lethal withcdc12formin Cdc12 Synthetic LethalityPMID:24127216
synthetic lethal withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:19139265
synthetic lethal withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:20603077
synthetic lethal withchs2chitin synthase homolog Chs2 Synthetic LethalityPMID:16772338
synthetic growth defect withclp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic Growth DefectPMID:15265986
enhances phenotype ofcyk3cytokinesis protein Cyk3 (predicted) Phenotypic EnhancementPMID:22573890
synthetic growth defect withcyk3cytokinesis protein Cyk3 (predicted) Synthetic Growth DefectPMID:22573890
synthetic lethal withfor3formin For3 Synthetic LethalityPMID:24127216
phenotype enhanced bygef2RhoGEF Gef2 Phenotypic EnhancementPMID:23966468
rescued byhsp9heat shock protein Hsp9 Phenotypic SuppressionPMID:8654972
synthetic growth defect withimp2contractile ring protein Imp2 Synthetic Growth DefectPMID:19139265
growth defect in presence of overexpressedimp2contractile ring protein Imp2 Dosage Growth DefectPMID:9786952
synthetic growth defect withimp2contractile ring protein Imp2 Synthetic Growth DefectPMID:9786952
rescued bykin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:23294323
synthetic growth defect withmob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Synthetic Growth DefectPMID:10769201
synthetically rescued bymyo2myosin II heavy chain Synthetic RescuePMID:10769212
synthetic lethal withmyo2myosin II heavy chain Synthetic LethalityPMID:10022828
synthetic lethal withmyp2myosin II heavy chain Myo3 Synthetic LethalityPMID:20739711
phenotype enhanced bynod1medial cortical node Gef2-related protein protein Nod1 Phenotypic EnhancementPMID:23966468
synthetic lethal withpxl1paxillin-like protein Pxl1 Synthetic LethalityPMID:18256290
synthetic lethal withrng2IQGAP Synthetic LethalityPMID:9635188
synthetically rescued byrng2IQGAP Synthetic RescuePMID:23615450
synthetic growth defect withscw1RNA-binding protein Scw1 Synthetic Growth DefectPMID:20739711
enhances phenotype ofsep1forkhead transcription factor Sep1 Phenotypic EnhancementPMID:8505375
synthetic lethal withspn1mitotic septin Spn1 Synthetic LethalityPMID:20739711
synthetic growth defect withspn4mitotic septin Spn4 Synthetic Growth DefectPMID:20739711
External References
Database Identifier Description
NBRP SPAP8A3.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAP8A3.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAP8A3.08 BioGRID Interaction Datasets
Expression Viewer SPAP8A3.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAP8A3.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAP8A3.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAP8A3.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAP8A3.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAP8A3.08 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAP8A3.08 Cell Cycle Data
GEO SPAP8A3.08 GEO profiles
PInt SPAP8A3.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAP8A3.08 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAP8A3.08 Fission yeast phenotypic data & analysis
UniProtKB/SwissProtQ09196Myosin regulatory light chain cdc4
ModBaseQ09196Database of comparative protein structure models
STRINGQ09196Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594947myosin II light chain
RefSeq mRNANM_001020378972h- myosin II light chain (cdc4), mRNA
ePDB1GGWThe European PDB
PDB1GGWPDB
PDBsum1GGWPDBsum
European Nucleotide ArchiveAAA67467.1ENA Protein Mapping
European Nucleotide ArchiveCAB55175.1ENA Protein Mapping
UniParcUPI000012F20EUniProt Archive

Literature for cdc4

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015