cdc4 (SPAP8A3.08)


Gene Standard Namecdc4 Characterisation Statuspublished
Systematic IDSPAP8A3.08 Feature Typeprotein coding
Synonyms Name Description
Productmyosin II light chain Product Size141aa, 15.66 kDa
Genomic Location Chromosome I, 5329177-5331057 (1881nt); CDS:5329218-5329906 (689nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005509calcium ion bindingIEAIPR002048GO_REF:000000252
GO:0005515protein bindingIPImyo2PMID:11069761861
IPIpik1PMID:11087749
IPImyp2PMID:10769212
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:2000689actin filament organization involved in cytokinetic actomyosin contractile ring assemblyIMPPMID:88347986
GO:0000915actomyosin contractile ring assemblyIMPPMID:762256526
requiredIMPPMID:9105045
GO:0071574protein localization to medial cortex6
localizes rng2IMPPMID:21376595
localizes cdc15IMPPMID:21376595
localizes rlc1IMPPMID:21376595
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005826actomyosin contractile ringIDAPMID:762256531
colocalizes_withIDAPMID:9105045
GO:0032153cell division siteIDAPMID:16823372295
GO:0005829cytosolIDAPMID:168233722316
GO:0071341medial cortical nodeIDAPMID:2137659510
GO:0016460myosin II complexIDAPMID:155049134
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopycdc4ΔNullPMID:204732891315
Microscopycdc4ΔNullPECO:0000005, PECO:0000137PMID:23697806
not recorded (unrecorded)PMID:11087750

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002425abnormal actin cable organization during mitosisMicroscopycdc4Not specifiedPECO:0000004PMID:88347982
FYPO:0000161abnormal actomyosin contractile ring assemblyMicroscopycdc4-8PECO:0000004PMID:76225658
Microscopycdc4-31PECO:0000004PMID:7622565
FYPO:0000731abnormal protein localization to medial cortex during vegetative growth15
affecting cdc15Microscopycdc4ΔNullPMID:21376595
affecting rlc1Microscopycdc4ΔNullPMID:21376595
affecting rng2Microscopycdc4ΔNullPMID:21376595
FYPO:0003289abnormal protein localization to medial cortical node1
affecting cdc12Microscopycdc4-377Not specifiedPMID:9105045
FYPO:0000117abnormal septationMicroscopycdc4-31PECO:0000004PMID:762256540
Microscopycdc4-8PECO:0000004PMID:7622565
FYPO:0002561abolished protein localization to actomyosin contractile ring10
affecting pxl1Microscopycdc4-8Not specifiedPECO:0000004PMID:18256290
penetrance FYPO_EXT:0000001
affecting chs2
Microscopycdc4-8Not specifiedPECO:0000004PMID:16772338
FYPO:0002555abolished protein localization to medial cortex during vegetative growth8
affecting pxl1Microscopycdc4-8Not specifiedPECO:0000004PMID:18256290
FYPO:0000272abolished septum formationMicroscopycdc4-377Not specifiedPECO:0000004PMID:88347986
FYPO:0001008contractile ring absentMicroscopycdc4-377Not specifiedPECO:0000004PMID:88347984
FYPO:0000133elongated multinucleate vegetative cellMicroscopycdc4-8PECO:0000004PMID:762256525
FYPO:0000252increased spontaneous diploidizationMicroscopycdc4-377Not specifiedPECO:0000004PMID:883479821
FYPO:0000049inviable cellMicroscopycdc4ΔNullPMID:76225651381
FYPO:0000779inviable elongated dumbbell-shaped cellMicroscopycdc4ΔNullPMID:76225656
FYPO:0001493inviable elongated multinucleate vegetative cellMicroscopycdc4-377Not specifiedPECO:0000004PMID:883479810
FYPO:0001006inviable, elongated, multinucleate dumbbell-shaped cellMicroscopycdc4-8Not specifiedPECO:0000004PMID:64907494
Microscopycdc4-31EndogenousPECO:0000004PMID:958201
Microscopycdc4-8EndogenousPECO:0000004PMID:958201
FYPO:0002489spore lysisMicroscopycdc4ΔNullPECO:0000005, PECO:0000137PMID:236978062
Microscopycdc4ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in chs2 chitin synthase homolog Chs2 PMID:16772338
FYPO affected by mutation in pxl1 paxillin-like protein Pxl1 PMID:18256290
FYPO affected by mutation in rng2 IQGAP PMID:21376595
GO regulated by ace2 transcription factor Ace2 PMID:15195092
GO localized by rng2 IQGAP PMID:21376595
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
153291775329248
253293015329432
353295065329646
453297135329805
553298785331057

UTRs

Region Coordinates Reference
five_prime_UTR5329177..5329217PMID:21511999
three_prime_UTR5329907..5331057PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13405 Pfam IPR002048 EF-hand domain 9 34 6
PF13499 Pfam IPR011992 EF-hand domain pair 83 138 3
SM00054 SMART IPR002048 EF-hand domain 7 35 14
SM00054 SMART IPR002048 EF-hand domain 78 106 14
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 87 99 12
PS50222 Prosite Profiles IPR002048 EF-hand domain 74 109 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 3 38 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 125 141 20
PTHR23048 HMMPANTHER 8 141 1
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 8 81 20
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 82 138 20
SSF47473 SuperFamily 7 138 21

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276

Protein Properties

Ave. residue weight 111.07 Da
Charge -11.00
Isoelectric point 4.16
Molecular weight 15.66 kDa
Number of residues 141
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
77000during GO:0072690PECO:0000012,
PECO:0000005
experimental evidencePMID:16224022
19695.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
16916.2during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
98134during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
114591during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
122236during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
110981during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
116485during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
7.8during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
2.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
myo2myosin II heavy chain Synthetic RescuePMID:10769212
Synthetic LethalityPMID:10022828
ase1antiparallel microtubule cross-linking factor Ase1 Synthetic LethalityPMID:15647375
rng2IQGAP Synthetic LethalityPMID:9635188
Synthetic RescuePMID:23615450
kin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:23294323
spn1septin Spn1 Synthetic LethalityPMID:20739711
imp2contractile ring protein Imp2 Synthetic Growth DefectPMID:19139265
Dosage Growth DefectPMID:9786952
Synthetic Growth Defect
spn4septin Spn4 Synthetic Growth DefectPMID:20739711
cam2myosin I light chain Cam2 Phenotypic SuppressionPMID:21693583
Synthetic Rescue
hsp9heat shock protein Hsp9 Phenotypic SuppressionPMID:8654972
clp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic Growth DefectPMID:15265986
chs2chitin synthase homolog Chs2 Synthetic LethalityPMID:16772338
acp2F-actin capping protein beta subunit Acp2 Phenotypic EnhancementPMID:16866873
Synthetic Lethality
Synthetic Growth DefectPMID:15743909
blt1Blt1 Synthetic Growth DefectPMID:24790095
act1actin Act1 Synthetic RescuePMID:10371213
Synthetic Lethality
Synthetic Growth DefectPMID:23589458
Synthetic Lethality
acp1F-actin capping protein alpha subunit Synthetic LethalityPMID:16866873
cyk3cytokinesis protein Cyk3 (predicted) Synthetic Growth DefectPMID:22573890
Phenotypic Enhancement
mob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Synthetic Growth DefectPMID:10769201
adf1actin depolymerizing factor, cofilin Synthetic LethalityPMID:16467379
Phenotypic Enhancement
cdc12formin Cdc12 Synthetic LethalityPMID:24127216
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:10503548
scw1RNA-binding protein Scw1 Synthetic Growth DefectPMID:20739711
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:19139265
Synthetic LethalityPMID:20603077
myp2myosin II heavy chain Myo3 Synthetic LethalityPMID:20739711
for3formin For3 Synthetic LethalityPMID:24127216
sep1forkhead transcription factor Sep1 Phenotypic EnhancementPMID:8505375
pxl1paxillin-like protein Pxl1 Synthetic LethalityPMID:18256290
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
myo2myosin II heavy chain Affinity Capture-WesternPMID:10769212
Two-hybrid
Affinity Capture-WesternPMID:15504913
Affinity Capture-WesternPMID:11087749
Affinity Capture-WesternPMID:11056543
Affinity Capture-WesternPMID:10022828
Affinity Capture-WesternPMID:21422229
Affinity Capture-WesternPMID:11942609
Reconstituted ComplexPMID:21693583
rng2IQGAP Two-hybridPMID:21422229
Affinity Capture-MSPMID:20603077
myp2myosin II heavy chain Myo3 Affinity Capture-WesternPMID:10769212
Two-hybrid
rlc1myosin II regulatory light chain Rlc1 Affinity Capture-WesternPMID:11069761
Affinity Capture-WesternPMID:11056543
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
mid1medial ring protein Mid1 Affinity Capture-WesternPMID:21422229
Affinity Capture-WesternPMID:22427686
pik11-phosphatidylinositol 4-kinase Pik1 Reconstituted ComplexPMID:11087749
Reconstituted ComplexPMID:21693583
External References
Database Identifier Description
NBRP SPAP8A3.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAP8A3.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAP8A3.08 BioGRID Interaction Datasets
Expression Viewer SPAP8A3.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAP8A3.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAP8A3.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAP8A3.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAP8A3.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAP8A3.08 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAP8A3.08 Cell Cycle Data
GEO SPAP8A3.08 GEO profiles
PInt SPAP8A3.08 Protein-Protein Interaction Predictor (Bähler Lab)
EntrezGene2541699myosin II light chain
WikiGene2541699myosin II light chain
UniProtKB/SwissProtQ09196Myosin regulatory light chain cdc4
ModBaseQ09196Database of comparative protein structure models
STRINGQ09196Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594947myosin II light chain
RefSeq mRNANM_001020378972h- myosin II light chain (cdc4), mRNA
ePDB1GGWThe European PDB
PDB1GGWPDB
PDBsum1GGWPDBsum
European Nucleotide ArchiveAAA67467ENA Protein Mapping
European Nucleotide ArchiveAAA67467.1ENA Protein Mapping
European Nucleotide ArchiveCAB55175ENA Protein Mapping
European Nucleotide ArchiveCAB55175.1ENA Protein Mapping
UniParcUPI000012F20EUniProt Archive

Literature for cdc4

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014