cdc4 (SPAP8A3.08)

Gene Standard Namecdc4 ChromosomeI
Systematic IDSPAP8A3.08 Gene Start5329177
Synonyms Gene End5331057
Productmyosin II light chain Gene Length1881
Feature Typeprotein coding CDS Start5329218
Name Description CDS End5329906
Characterisation Statuspublished CDS Length689

Ensembl Gene Location
  1. ski7
  2. SPAP8A3.06
  3. SPAP8A3.07c
  4. SPNCRNA.1072
  5. SPNCRNA.525
  6. SPNCRNA.1073
  7. cdc4
  8. paa1
  9. SPAP8A3.10
  10. SPAP8A3.11c
  11. tpp2
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000117abnormal septationMicroscopycdc4-8 (unknown)unknownPECO:0000004PMID:7622565
Microscopycdc4-31 (unknown)unknownPECO:0000004PMID:7622565
FYPO:0000133elongated multinucleate cellMicroscopycdc4-8 (unknown)unknownPECO:0000004PMID:7622565
FYPO:0000161abnormal actomyosin contractile ring assemblyMicroscopycdc4-31 (unknown)unknownPECO:0000004PMID:7622565
Microscopycdc4-8 (unknown)unknownPECO:0000004PMID:7622565
FYPO:0000252increased spontaneous diploidizationMicroscopycdc4-377 (unknown)unknownNot specifiedPECO:0000004PMID:8834798
FYPO:0000731abnormal protein localization to medial cortex during vegetative growthMicroscopycdc4delta (deletion)deletionPMID:21376595
has_input rlc1
has_input rng2Microscopycdc4delta (deletion)deletionPMID:21376595
has_input cdc15Microscopycdc4delta (deletion)deletionPMID:21376595
FYPO:0000779dumbbell-shaped cellMicroscopycdc4delta (deletion)deletionPMID:7622565
FYPO:0001006multinucleate dumbbell-shaped cellMicroscopycdc4-8 (unknown)unknownNot specifiedPECO:0000004PMID:6490749
Microscopycdc4-31 (unknown)unknownEndogenousPECO:0000004PMID:958201
Microscopycdc4-8 (unknown)unknownEndogenousPECO:0000004PMID:958201
FYPO:0001493inviable elongated multinucleate vegetative cellMicroscopycdc4-377 (unknown)unknownNot specifiedPECO:0000004PMID:8834798
FYPO:0002061inviable vegetative cell populationno_name (unrecorded)unrecordedPMID:11087750
Microscopycdc4delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0005509calcium ion bindingIEAIPR002048, IPR011992GO_REF:0000002
GO:0005515protein bindingIPImyp2PMID:10769212
IPIpik1PMID:11087749
IPImyo2PMID:11069761
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0000915cytokinesis, actomyosin contractile ring assemblyIMPPMID:7622565
requiredIMPPMID:9105045
GO:0071574protein localization to medial cortex
has_input cdc15IMPPMID:21376595
has_input rng2IMPPMID:21376595
has_input rlc1IMPPMID:21376595
GO:2000689actin filament organization involved in cytokinetic actomyosin contractile ring assemblyIMPPMID:8834798
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0005634nucleusIDAPMID:16823372
GO:0005826actomyosin contractile ringIDAPMID:7622565
colocalizes_withIDAPMID:9105045
GO:0005829cytosolIDAPMID:16823372
GO:0016460myosin II complexIDAPMID:15504913
GO:0032153cell division siteIDAPMID:16823372
GO:0071341medial cortical node
localization_dependent_on rng2IDAPMID:21376595
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
153291775329248
253293015329432
353295065329646
453297135329805
553298785331057

References

Region Start End Reference
three_prime_UTR53299075331057PMID:21511999
five_prime_UTR53291775329217PMID:21511999
three_prime_UTR53299075331057PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF13405 Pfam EF-hand domain 9 34 6
PF13499 Pfam EF-hand-like domain 83 138 2
SM00054 IPR002048 SMART EF-hand domain 7 35 14
SM00054 IPR002048 SMART EF-hand domain 78 106 14
PS50222 IPR018249 Prosite Profiles EF-hand domain 74 109 20
PS50222 IPR018249 Prosite Profiles EF-hand domain 3 38 20
PS50222 IPR018249 Prosite Profiles EF-hand domain 125 141 20
PS00018 IPR018247 Prosite Patterns EF-hand domain 87 99 12
G3DSA:1.10.238.10 IPR011992 Gene3D 8 81 20
G3DSA:1.10.238.10 IPR011992 Gene3D 82 138 20
SSF47473 SuperFamily 7 138 21
PTHR23048 hmmpanther 8 141 1
PTHR23048:SF0 hmmpanther 8 141 1

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residueevidenceInferred from Sequence or Structural Similarity

Manually Curated Family or Sub-families

Term IDTerm NameReferenceAll Genes
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276

Protein Properties

Ave. residue weight 111.07 Da
Charge -11.00
Isoelectric point 4.16
Molecular weight 15.66 kDa
Number of residues 141
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencesingle-celled organism vegetative growth phasePECO:0000201,
PECO:0000005		single_cell77000PMID:16224022
experimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide16916.2PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide19695.2PMID:23101633
RNA levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide2.1PMID:23101633
experimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide7.8PMID:23101633
Species Distribution
DescriptionQualifierReference
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic Growth DefectPMID:15265986
pxl1paxillin-like protein Pxl1 Synthetic LethalityPMID:18256290
imp2contractile ring protein Imp2 Synthetic Growth DefectPMID:9786952
Dosage Growth Defect
Synthetic Growth DefectPMID:19139265
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:20603077
Synthetic LethalityPMID:19139265
acp2F-actin capping protein beta subunit (predicted) Synthetic Growth DefectPMID:15743909
Synthetic LethalityPMID:16866873
Phenotypic Enhancement
rng2IQGAP Synthetic LethalityPMID:9635188
scw1RNA-binding protein Scw1 Synthetic Growth DefectPMID:20739711
spn1septin Spn1 Synthetic LethalityPMID:20739711
cyk3cytokinesis protein Cyk3 (predicted) Phenotypic EnhancementPMID:22573890
Synthetic Growth Defect
sep1forkhead transcription factor Sep1 Phenotypic EnhancementPMID:8505375
myo2myosin II heavy chain Synthetic RescuePMID:10769212
Synthetic LethalityPMID:10022828
act1actin Act1 Synthetic RescuePMID:10371213
Synthetic Lethality
adf1actin depolymerizing factor, cofilin Synthetic LethalityPMID:16467379
Phenotypic Enhancement
spn4septin Spn4 Synthetic Growth DefectPMID:20739711
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:10503548
mob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Synthetic Growth DefectPMID:10769201
acp1F-actin capping protein alpha subunit Synthetic LethalityPMID:16866873
hsp9heat shock protein Hsp9 Phenotypic SuppressionPMID:8654972
chs2chitin synthase homolog Chs2 Synthetic LethalityPMID:16772338
myp2myosin II heavy chain Myo3 Synthetic LethalityPMID:20739711
ase1antiparallel microtubule cross-linking factor Ase1 Synthetic LethalityPMID:15647375
kin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:23294323
cam2myosin I light chain Cam2 Phenotypic SuppressionPMID:21693583
Synthetic Rescue
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
pik11-phosphatidylinositol 4-kinase Pik1 Reconstituted ComplexPMID:21693583
Reconstituted ComplexPMID:11087749
cdc12formin Cdc12 Co-localizationPMID:9105045
chs2chitin synthase homolog Chs2 Co-localizationPMID:16772338
myp2myosin II heavy chain Myo3 Two-hybridPMID:10769212
Affinity Capture-Western
rng2IQGAP Two-hybridPMID:21422229
Affinity Capture-MSPMID:20603077
mid1medial ring protein Mid1 Affinity Capture-WesternPMID:21422229
Affinity Capture-WesternPMID:22427686
myo2myosin II heavy chain Affinity Capture-WesternPMID:21422229
Affinity Capture-WesternPMID:10769212
Two-hybrid
Reconstituted ComplexPMID:21693583
Affinity Capture-WesternPMID:10022828
Affinity Capture-WesternPMID:11056543
Affinity Capture-WesternPMID:11087749
Affinity Capture-WesternPMID:15504913
Affinity Capture-WesternPMID:11942609
rlc1myosin II regulatory light chain Rlc1 Affinity Capture-WesternPMID:11056543
Affinity Capture-WesternPMID:11069761
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External References
Database Identifier Description
NBRP SPAP8A3.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAP8A3.08 Retrieval of eukaryotic orthologs
BioGrid SPAP8A3.08 BioGRID Interaction Datasets
Bähler Lab SPAP8A3.08 Cell Cycle Expression Profile
Bähler Lab SPAP8A3.08 Meiosis/Sporulation Expression Profies
Bähler Lab SPAP8A3.08 Pheromone response/mating expression profiles
Bähler Lab SPAP8A3.08 Environmental stress expression profiles
Bähler Lab SPAP8A3.08 Bähler Lab Transcriptome Viewer
Cyclebase SPAP8A3.08 Cell Cycle Data
PInt SPAP8A3.08 Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAP8A3.08 Entrez Gene
EntrezGene2541699myosin II light chain
Uniprot_genenameCDC4
WikiGene2541699myosin II light chain
UniProtKB/SwissProtQ09196Myosin regulatory light chain cdc4
ModBaseQ09196Database of comparative protein structure models
Pfam Protein DomainsQ09196Pfam Domain Arrangement
RefSeq PeptideNP_594947myosin II light chain
RefSeq mRNANM_001020378972h- myosin II light chain (cdc4), mRNA
European Nucleotide ArchiveAAA67467ENA Protein Mapping
European Nucleotide ArchiveCAB55175ENA Protein Mapping
UniParcUPI000012F20EUniProt Archive

Literature for cdc4
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013