cdc4 (SPAP8A3.08)

Gene Standard Namecdc4 Characterisation Statuspublished
Systematic IDSPAP8A3.08 Feature Typeprotein coding
Synonyms Name Description
Productmyosin II light chain Product Size141aa, 15.66 kDa
Genomic Location Chromosome I, 5329177-5331057 (1881nt); CDS:5329218-5329906 (689nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
metal ion binding747
Annotation ExtensionEvidenceWith/FromReference
protein binding848
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitotic actomyosin contractile ring assembly28
Annotation ExtensionEvidenceWith/FromReference
mitotic actomyosin contractile ring assembly actin filament organization3
Annotation ExtensionEvidenceWith/FromReference
protein localization to medial cortex6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actomyosin contractile ring32
Annotation ExtensionEvidenceWith/FromReference
cell division site299
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
medial cortical node10
Annotation ExtensionEvidenceWith/FromReference
myosin II complex4
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationcdc4ΔNull1427
not recorded (unrecorded)

Cell Phenotype

Term NameAlleleExpressionCount
abnormal actin cable organization during mitosiscdc4Not specified2
abnormal actomyosin contractile ring assemblycdc4-3167
abnormal protein localization to medial cortex during vegetative growth17
affecting cdc15cdc4ΔNull
affecting rng2cdc4ΔNull
affecting rlc1cdc4ΔNull
abnormal protein localization to medial cortical node3
affecting cdc12cdc4-377Not specified
abnormal septationcdc4-31117
abolished protein localization to actomyosin contractile ring11
penetrance FYPO_EXT:0000001, affecting chs2cdc4-8Not specified
affecting pxl1cdc4-8Not specified
abolished protein localization to medial cortex during vegetative growth10
affecting pxl1cdc4-8Not specified
abolished septum formationcdc4-377Not specified58
actomyosin contractile ring absentcdc4-377Not specified65
elongated multinucleate multiseptate vegetative cell, septa between nucleicdc4-8Not specified15
elongated multinucleate vegetative cellcdc4-834
increased spontaneous diploidizationcdc4-377Not specified24
inviable cellcdc4ΔNull1458
inviable elongated dumbbell-shaped cellcdc4ΔNull6
inviable elongated multinucleate vegetative cellcdc4-377Not specified12
inviable, elongated, multinucleate dumbbell-shaped cellcdc4-31Endogenous4
cdc4-8Not specified
spore lysiscdc4ΔNull3
Target Of
FYPO affected by mutation in chs2 chitin synthase homolog Chs2 PMID:16772338
FYPO affected by mutation in rng2 IQGAP PMID:21376595
GO localized by rng2 IQGAP PMID:21376595
GO regulated by ace2 transcription factor Ace2 PMID:15195092
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13499 Pfam IPR011992 EF-hand domain pair 83 138 7
PF13405 Pfam IPR002048 EF-hand domain 9 34 4
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 87 99 12
PS50222 Prosite Profiles IPR002048 EF-hand domain 125 141 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 74 109 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 3 38 20
PTHR23048 HMMPANTHER 8 141 1 Gene3D IPR011992 EF-hand domain pair 8 81 20 Gene3D IPR011992 EF-hand domain pair 82 138 20
SSF47473 SuperFamily 7 138 21

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276

Protein Properties

Ave. residue weight 111.07 Da
Charge -11.00
Isoelectric point 4.16
Molecular weight 15.66 kDa
Number of residues 141
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
122236during GO:0000080PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
114591during GO:0000084PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
116485during GO:0000085PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
110981during GO:0000087PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
98134during GO:0072690PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
16916.2during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
77000during GO:0072690PECO:0000005,
single cellexperimental evidencePMID:16224022
19695.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7.8during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
2.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633
Species Distribution
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
myo2myosin II heavy chain Affinity Capture-WesternPMID:10769212
Affinity Capture-WesternPMID:15504913
Affinity Capture-WesternPMID:11087749
Affinity Capture-WesternPMID:11056543
Affinity Capture-WesternPMID:10022828
Affinity Capture-WesternPMID:21422229
Affinity Capture-WesternPMID:11942609
Reconstituted ComplexPMID:21693583
rng2IQGAP Two-hybridPMID:21422229
Affinity Capture-MSPMID:20603077
myp2myosin II heavy chain Myo3 Affinity Capture-WesternPMID:10769212
rlc1myosin II regulatory light chain Rlc1 Affinity Capture-WesternPMID:11069761
Affinity Capture-WesternPMID:11056543
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
mid1medial ring protein Mid1 Affinity Capture-WesternPMID:21422229
Affinity Capture-WesternPMID:22427686
pik11-phosphatidylinositol 4-kinase Pik1 Reconstituted ComplexPMID:11087749
Reconstituted ComplexPMID:21693583
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
myo2myosin II heavy chain Synthetic RescuePMID:10769212
Synthetic LethalityPMID:10022828
ase1antiparallel microtubule cross-linking factor Ase1 Synthetic LethalityPMID:15647375
rng2IQGAP Synthetic LethalityPMID:9635188
Synthetic RescuePMID:23615450
nod1medial cortical node Gef2-related protein protein Nod1 Phenotypic EnhancementPMID:23966468
kin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:23294323
spn1septin Spn1 Synthetic LethalityPMID:20739711
imp2contractile ring protein Imp2 Synthetic Growth DefectPMID:19139265
Synthetic Growth DefectPMID:9786952
Dosage Growth Defect
spn4septin Spn4 Synthetic Growth DefectPMID:20739711
cam2myosin I light chain Cam2 Phenotypic SuppressionPMID:21693583
Synthetic Rescue
hsp9heat shock protein Hsp9 Phenotypic SuppressionPMID:8654972
clp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic Growth DefectPMID:15265986
chs2chitin synthase homolog Chs2 Synthetic LethalityPMID:16772338
acp2F-actin capping protein beta subunit Acp2 Phenotypic EnhancementPMID:16866873
Synthetic Lethality
Synthetic Growth DefectPMID:15743909
blt1ubiquitin domain-like protein Blt1 Synthetic Growth DefectPMID:24790095
act1actin Act1 Synthetic RescuePMID:10371213
Synthetic Lethality
Synthetic Growth DefectPMID:23589458
Synthetic Lethality
acp1F-actin capping protein alpha subunit Synthetic LethalityPMID:16866873
cyk3cytokinesis protein Cyk3 (predicted) Synthetic Growth DefectPMID:22573890
Phenotypic Enhancement
mob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Synthetic Growth DefectPMID:10769201
adf1actin depolymerizing factor, cofilin Phenotypic EnhancementPMID:16467379
Synthetic Lethality
cdc12formin Cdc12 Synthetic LethalityPMID:24127216
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:10503548
scw1RNA-binding protein Scw1 Synthetic Growth DefectPMID:20739711
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:19139265
Synthetic LethalityPMID:20603077
myp2myosin II heavy chain Myo3 Synthetic LethalityPMID:20739711
gef2RhoGEF Gef2 Phenotypic EnhancementPMID:23966468
for3formin For3 Synthetic LethalityPMID:24127216
sep1forkhead transcription factor Sep1 Phenotypic EnhancementPMID:8505375
pxl1paxillin-like protein Pxl1 Synthetic LethalityPMID:18256290
External References
Database Identifier Description
NBRP SPAP8A3.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAP8A3.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAP8A3.08 BioGRID Interaction Datasets
Expression Viewer SPAP8A3.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAP8A3.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAP8A3.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAP8A3.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAP8A3.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAP8A3.08 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAP8A3.08 Cell Cycle Data
GEO SPAP8A3.08 GEO profiles
PInt SPAP8A3.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAP8A3.08 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAP8A3.08 Fission yeast phenotypic data & analysis
UniProtKB/SwissProtQ09196Myosin regulatory light chain cdc4
ModBaseQ09196Database of comparative protein structure models
STRINGQ09196Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594947myosin II light chain
RefSeq mRNANM_001020378972h- myosin II light chain (cdc4), mRNA
ePDB1GGWThe European PDB
European Nucleotide ArchiveAAA67467.1ENA Protein Mapping
European Nucleotide ArchiveCAB55175.1ENA Protein Mapping
UniParcUPI000012F20EUniProt Archive

Literature for cdc4

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015