cdc4 (SPAP8A3.08)


Gene Standard Namecdc4 Characterisation Statuspublished
Systematic IDSPAP8A3.08 Feature Typeprotein coding
Synonyms Name Description
Productmyosin II light chain Product Size141aa, 15.66 kDa
Genomic Location Chromosome I, 5329177-5331057 (1881nt); CDS:5329218-5329906 (689nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005509calcium ion bindingIEAIPR002048GO_REF:000000252
GO:0005515protein bindingIPIpik1PMID:11087749887
IPImyo2PMID:11069761
IPImyp2PMID:10769212
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:2000689actin filament organization involved in cytokinetic actomyosin contractile ring assemblyIMPPMID:88347986
GO:0000915cytokinesis, actomyosin contractile ring assemblyIMPPMID:762256527
requiredIMPPMID:9105045
GO:0071574protein localization to medial cortex5
localizes rng2IMPPMID:21376595
localizes rlc1IMPPMID:21376595
localizes cdc15IMPPMID:21376595
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005826actomyosin contractile ringIDAPMID:762256528
colocalizes_withIDAPMID:9105045
GO:0032153cell division siteIDAPMID:16823372292
GO:0005829cytosolIDAPMID:168233722317
GO:0071341medial cortical nodeIDAPMID:2137659510
GO:0016460myosin II complexIDAPMID:155049134
GO:0005634nucleusIDAPMID:168233722730
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopycdc4ΔNullPECO:0000005, PECO:0000137PMID:236978061309
Microscopycdc4ΔNullPMID:20473289
no_namePMID:11087750

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002425abnormal actin cable organization during mitosisMicroscopycdc4Not specifiedPECO:0000004PMID:88347982
FYPO:0000161abnormal actomyosin contractile ring assemblyMicroscopycdc4-31PECO:0000004PMID:76225658
Microscopycdc4-8PECO:0000004PMID:7622565
FYPO:0000731abnormal protein localization to medial cortex during vegetative growthMicroscopycdc4ΔNullPMID:213765955
affecting cdc15
affecting rlc1Microscopycdc4ΔNullPMID:21376595
affecting rng2Microscopycdc4ΔNullPMID:21376595
FYPO:0000117abnormal septationMicroscopycdc4-8PECO:0000004PMID:762256536
Microscopycdc4-31PECO:0000004PMID:7622565
FYPO:0000272abolished septum formationMicroscopycdc4-377Not specifiedPECO:0000004PMID:88347985
FYPO:0001008contractile ring absentMicroscopycdc4-377Not specifiedPECO:0000004PMID:88347984
FYPO:0000133elongated multinucleate cellMicroscopycdc4-8PECO:0000004PMID:762256516
FYPO:0000252increased spontaneous diploidizationMicroscopycdc4-377Not specifiedPECO:0000004PMID:88347989
FYPO:0000049inviable cellMicroscopycdc4ΔNullPMID:76225651356
FYPO:0000779inviable elongated dumbbell-shaped cellMicroscopycdc4ΔNullPMID:76225656
FYPO:0001493inviable elongated multinucleate vegetative cellMicroscopycdc4-377Not specifiedPECO:0000004PMID:883479810
FYPO:0001006inviable, elongated, multinucleate dumbbell-shaped cellMicroscopycdc4-8EndogenousPECO:0000004PMID:9582014
Microscopycdc4-31EndogenousPECO:0000004PMID:958201
Microscopycdc4-8Not specifiedPECO:0000004PMID:6490749
FYPO:0002489spore lysisMicroscopycdc4ΔNullPECO:0000005, PECO:0000137PMID:236978062
Microscopycdc4ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
RelationshipGeneProductReference
regulated by ace2 transcription factor Ace2 PMID:15195092
localised by rng2 IQGAP PMID:21376595
localized by rng2 IQGAP PMID:21376595
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
153291775329248
253293015329432
353295065329646
453297135329805
553298785331057

UTRs

Region Start End Reference
three_prime_UTR53299075331057PMID:21511999
five_prime_UTR53291775329217PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13405 Pfam IPR002048 EF-hand domain 9 34 6
PF13499 Pfam IPR011992 EF-hand domain pair 83 138 3
SM00054 SMART IPR002048 EF-hand domain 7 35 14
SM00054 SMART IPR002048 EF-hand domain 78 106 14
PS50222 Prosite Profiles IPR002048 EF-hand domain 74 109 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 3 38 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 125 141 20
PS00018 Prosite Patterns IPR018247 EF-hand domain 87 99 12
PTHR23048 HMMPANTHER 8 141 1
PTHR23048:SF0 HMMPANTHER 8 141 1
G3DSA:1.10.238.10 Gene3D IPR011992 8 81 20
G3DSA:1.10.238.10 Gene3D IPR011992 82 138 20
SSF47473 SuperFamily 7 138 21

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276

Protein Properties

Ave. residue weight 111.07 Da
Charge -11.00
Isoelectric point 4.16
Molecular weight 15.66 kDa
Number of residues 141
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
77000during GO:0072690PECO:0000005,
PECO:0000012
single_cellexperimental evidencePMID:16224022
19695.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
16916.2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
7.8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
myo2myosin II heavy chain Synthetic RescuePMID:10769212
Synthetic LethalityPMID:10022828
ase1antiparallel microtubule cross-linking factor Ase1 Synthetic LethalityPMID:15647375
rng2IQGAP Synthetic LethalityPMID:9635188
Synthetic RescuePMID:23615450
kin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:23294323
spn1septin Spn1 Synthetic LethalityPMID:20739711
imp2contractile ring protein Imp2 Synthetic Growth DefectPMID:19139265
Synthetic Growth DefectPMID:9786952
Dosage Growth Defect
spn4septin Spn4 Synthetic Growth DefectPMID:20739711
cam2myosin I light chain Cam2 Synthetic RescuePMID:21693583
Phenotypic Suppression
hsp9heat shock protein Hsp9 Phenotypic SuppressionPMID:8654972
clp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic Growth DefectPMID:15265986
chs2chitin synthase homolog Chs2 Synthetic LethalityPMID:16772338
acp2F-actin capping protein beta subunit Acp2 Phenotypic EnhancementPMID:16866873
Synthetic Lethality
Synthetic Growth DefectPMID:15743909
act1actin Act1 Synthetic LethalityPMID:10371213
Synthetic Rescue
Synthetic LethalityPMID:23589458
Synthetic Growth Defect
acp1F-actin capping protein alpha subunit Synthetic LethalityPMID:16866873
cyk3cytokinesis protein Cyk3 (predicted) Phenotypic EnhancementPMID:22573890
Synthetic Growth Defect
mob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Synthetic Growth DefectPMID:10769201
adf1actin depolymerizing factor, cofilin Synthetic LethalityPMID:16467379
Phenotypic Enhancement
cdc12formin Cdc12 Synthetic LethalityPMID:24127216
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:10503548
scw1RNA-binding protein Scw1 Synthetic Growth DefectPMID:20739711
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:19139265
Synthetic LethalityPMID:20603077
myp2myosin II heavy chain Myo3 Synthetic LethalityPMID:20739711
for3formin For3 Synthetic LethalityPMID:24127216
sep1forkhead transcription factor Sep1 Phenotypic EnhancementPMID:8505375
pxl1paxillin-like protein Pxl1 Synthetic LethalityPMID:18256290
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
myo2myosin II heavy chain Affinity Capture-WesternPMID:10769212
Two-hybrid
Affinity Capture-WesternPMID:15504913
Affinity Capture-WesternPMID:11087749
Affinity Capture-WesternPMID:11056543
Affinity Capture-WesternPMID:10022828
Affinity Capture-WesternPMID:21422229
Affinity Capture-WesternPMID:11942609
Reconstituted ComplexPMID:21693583
rng2IQGAP Two-hybridPMID:21422229
Affinity Capture-MSPMID:20603077
myp2myosin II heavy chain Myo3 Affinity Capture-WesternPMID:10769212
Two-hybrid
rlc1myosin II regulatory light chain Rlc1 Affinity Capture-WesternPMID:11069761
Affinity Capture-WesternPMID:11056543
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
mid1medial ring protein Mid1 Affinity Capture-WesternPMID:21422229
Affinity Capture-WesternPMID:22427686
pik11-phosphatidylinositol 4-kinase Pik1 Reconstituted ComplexPMID:11087749
Reconstituted ComplexPMID:21693583
External References
Database Identifier Description
NBRP SPAP8A3.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAP8A3.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAP8A3.08 BioGRID Interaction Datasets
Expression Viewer SPAP8A3.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAP8A3.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAP8A3.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAP8A3.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAP8A3.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAP8A3.08 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAP8A3.08 Cell Cycle Data
GEO SPAP8A3.08 GEO profiles
PInt SPAP8A3.08 Protein-Protein Interaction Predictor (Bähler Lab)
EntrezGene2541699myosin II light chain
WikiGene2541699myosin II light chain
UniProtKB/SwissProtQ09196Myosin regulatory light chain cdc4
ModBaseQ09196Database of comparative protein structure models
StringQ09196Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594947myosin II light chain
RefSeq mRNANM_001020378972h- myosin II light chain (cdc4), mRNA
ePDB1GGWThe European PDB
PDBsum1GGWPDBsum
European Nucleotide ArchiveAAA67467ENA Protein Mapping
European Nucleotide ArchiveCAB55175ENA Protein Mapping
UniParcUPI000012F20EUniProt Archive

Literature for cdc4

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014