sen2 (SPAPB17E12.07c)


Gene Standard Namesen2 Characterisation Statusbiological_role_inferred
Systematic IDSPAPB17E12.07c Feature Typeprotein coding
Synonyms Name Description
ProducttRNA-splicing endonuclease subunit catalytic subunit Sen2 (predicted) Product Size380aa, 44.61 kDa
Genomic Location Chromosome I, 1279149-1277120 (2030nt); CDS:1278974-1277785 (1190nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0016829lyase activityIEAUniProtKB-KW:KW-0456GO_REF:000003786
GO:0000213tRNA-intron endonuclease activityISOSGD:S000004095GO_REF:00000244
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0090502RNA phosphodiester bond hydrolysis, endonucleolyticISOSGD:S000004095GO_REF:000002424
GO:0000379tRNA-type intron splice site recognition and cleavageISOSGD:S000004095GO_REF:00000244
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmIDAPMID:168233724205
GO:0005741mitochondrial outer membraneISOSGD:S000004095GO_REF:000002444
GO:0000214tRNA-intron endonuclease complexISOSGD:S000004095GO_REF:00000244
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopysen2ΔNullPMID:204732891338
Microscopysen2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002187inviable normal volume spheroid vegetative cell49
penetrance FYPO_EXT:0000001Microscopysen2ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
112791491278140
212780921277120

UTRs

Region Coordinates Reference
five_prime_UTR1279149..1278975PMID:21511999
three_prime_UTR1277784..1277120PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01974 Pfam IPR006677 tRNA intron endonuclease, catalytic domain-like 251 333 2
TMhelix TMHMM 13 35 959
PTHR21227 HMMPANTHER 1 380 1
PTHR21227:SF0 HMMPANTHER 1 380 1
3.40.1350.10 Gene3D IPR011856 tRNA endonuclease-like domain 250 337 3
SSF53032 SuperFamily IPR006677 tRNA intron endonuclease, catalytic domain-like 250 335 3
PIRSF011789 PIRSF IPR016589 tRNA-splicing endonuclease, SEN2 subunit 1 380 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 117.39 Da
Charge 13.00
Isoelectric point 9.06
Molecular weight 44.61 kDa
Number of residues 380
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.76during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.19during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPAPB17E12.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAPB17E12.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAPB17E12.07c BioGRID Interaction Datasets
Expression Viewer SPAPB17E12.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAPB17E12.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAPB17E12.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAPB17E12.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAPB17E12.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAPB17E12.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAPB17E12.07c Cell Cycle Data
GEO SPAPB17E12.07c GEO profiles
PInt SPAPB17E12.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAPB17E12.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAPB17E12.07c Fission yeast phenotypic data & analysis
UniProtKB/SwissProtQ8TFH7Probable tRNA-splicing endonuclease subunit sen2
ModBaseQ8TFH7Database of comparative protein structure models
STRINGQ8TFH7Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_001018222tRNA-splicing endonuclease subunit catalytic subunit Sen2 (predicted)
RefSeq mRNANM_001018702972h- tRNA-splicing endonuclease subunit catalytic subunit Sen2 (predicted) (sen2), mRNA
European Nucleotide ArchiveCAD27500.1ENA Protein Mapping
UniParcUPI000006A3A1UniProt Archive

Literature for sen2

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014