ypt71 (SPAPB1A10.10c)


Gene Standard Nameypt71 Characterisation Statuspublished
Systematic IDSPAPB1A10.10c Feature Typeprotein coding
Synonyms Name Description
ProductGTPase Ypt71 Product Size208aa, 23.47 kDa
Genomic Location Chromosome I, 1881970-1880698 (1273nt); CDS:1881910-1880879 (1032nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
GTP binding112
Annotation ExtensionEvidenceWith/FromReference
GTPase activity62
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
endocytosis78
Annotation ExtensionEvidenceWith/FromReference
Golgi to vacuole transport18
Annotation ExtensionEvidenceWith/FromReference
intracellular protein transport342
Annotation ExtensionEvidenceWith/FromReference
negative regulation of vacuole fusion, non-autophagic1
Annotation ExtensionEvidenceWith/FromReference
small GTPase mediated signal transduction152
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
vacuolar membrane109
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
viable vegetative cell populationypt71ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
large vacuoles during vegetative growthypt71+ (wild type)Overexpression5
viable vegetative cell with normal cell morphologyypt71ΔNull3094
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1881970..1881830, 1881478..1881380, 1881325..1880698
mRNA1881970..1880698
5' UTR1881970..1881911PMID:21511999
CDS1881910..1881830, 1881478..1881380, 1881325..1880879
3' UTR1880878..1880698PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00071 Pfam IPR001806 Small GTPase superfamily 10 176 18
SM00174 SMART IPR003578 Small GTPase superfamily, Rho type 11 179 19
SM00173 SMART IPR020849 Small GTPase superfamily, Ras type 5 179 19
SM00175 SMART IPR003579 Small GTPase superfamily, Rab type 9 179 22
SM00176 SMART IPR002041 Ran GTPase 14 206 13
PS51419 Prosite Profiles 4 208 9
PTHR24073 HMMPANTHER 1 208 9
PTHR24073:SF57 HMMPANTHER 1 208 2
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 4 184 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 5 185 294
PR00449 PRINTS IPR001806 Small GTPase superfamily 9 30 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 154 176 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 32 48 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 50 72 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 115 128 19
TIGR00231 tigrfam IPR005225 Small GTP-binding protein domain 10 172 35

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001222Rab subfamilyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001222

Protein Properties

Ave. residue weight 112.86 Da
Charge -2.50
Isoelectric point 5.33
Molecular weight 23.47 kDa
Number of residues 208
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
30987during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
34422during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
29551during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
32586during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
32161during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
8075.49during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
15190.98during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.4during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.81during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
neurological disorders PMID:2329357899
Charcot-Marie-Tooth disease PMID:232935789
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
positive genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Positive GeneticPMID:22681890
positive genetic interaction withrhb1Rheb GTPase Rhb1 Positive GeneticPMID:22681890
positive genetic interaction withrxt2histone deacetylase complex subunit Rxt2 Positive GeneticPMID:22681890
negative genetic interaction withSPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:22681890
negative genetic interaction withSPCPB16A4.02cconserved fungal protein Negative GeneticPMID:22681890
negative genetic interaction withtax4autophagy/CVT pathway ENTH/VHS domain protein Tax4(predicted) Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAPB1A10.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAPB1A10.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAPB1A10.10c BioGRID Interaction Datasets
Expression Viewer SPAPB1A10.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAPB1A10.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAPB1A10.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAPB1A10.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAPB1A10.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAPB1A10.10c Transcriptome Viewer (Bähler Lab)
GEO SPAPB1A10.10c GEO profiles
PInt SPAPB1A10.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAPB1A10.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAPB1A10.10c Fission yeast phenotypic data & analysis
Cyclebase SPAPB1A10.10c.1 Cell Cycle Data
UniProtKB/SwissProtQ9HDY0GTP-binding protein ypt71
ModBaseQ9HDY0Database of comparative protein structure models
STRINGQ9HDY0Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593524GTPase Ypt71
RefSeq mRNANM_001018958972h- GTPase Ypt71 (ypt71), mRNA
European Nucleotide ArchiveCAC21483.1ENA Protein Mapping
UniParcUPI000006C051UniProt Archive

Literature for ypt71

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015