mcl1 (SPAPB1E7.02c)


Gene Standard Namemcl1 Characterisation Statuspublished
Systematic IDSPAPB1E7.02c Feature Typeprotein coding
Synonymsslr3 Name Description
ProductDNA polymerase alpha accessory factor Mcl1 Product Size815aa, 90.99 kDa
Genomic Location Chromosome I, 3285778-3283004 (2775nt); CDS:3285745-3283195 (2551nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding872
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viability depends on conditions

Population Phenotype

Term NameGenotypesCount
abnormal mitotic cell cycle arrest with unreplicated DNAmcl1+57
decreased cell population growth during glucose starvationmcl1Δ16
decreased cell population growth on glucose carbon sourcemcl1Δ320
decreased vegetative cell population growthmcl1-101 (C1477T, C2287T, G2303A)662
mcl1-1 (ntG372A)
mcl1-102 (Mcl1 amino acids 1-268 plus KPSLAMVIIVS at C-terminus)
mcl1Δ
inviable vegetative cell populationmcl1+1452
cos1-86 (Q765->stop)
cos1-22 (G1721A)
mcl1-1 (ntG372A)
cos1-7 (G724A)
cos1-38 (G1865A)
mcl1Δ
cos1-17 (T698A)
normal vegetative cell population growthmcl1-101 (C1477T, C2287T, G2303A)851
mcl1Δ
sensitive to bleomycinmcl1-101 (C1477T, C2287T, G2303A)80
sensitive to bortezomibmcl1Δ255
sensitive to camptothecinmcl1Δ248
sensitive to cisplatinmcl1Δ106
sensitive to clotrimazolemcl1Δ107
sensitive to cobaltmcl1Δ57
sensitive to DNA damage during mitotic S phasemcl1+1
sensitive to doxorubicinmcl1Δ95
sensitive to hydroxyureamcl1+580
mcl1Δ
mcl1-1 (ntG372A)
mcl1-101 (C1477T, C2287T, G2303A)
sensitive to ionizing radiation during vegetative growthmcl1-1 (ntG372A)73
sensitive to methyl methanesulfonatemcl1-1 (ntG372A)321
mcl1Δ
mcl1-101 (C1477T, C2287T, G2303A)
mcl1+
sensitive to sodium butyratemcl1Δ93
sensitive to tacrolimusmcl1Δ81
sensitive to terbinafinemcl1Δ103
sensitive to thiabendazolecos1-17 (T698A)327
mcl1-1 (ntG372A)
cos1-38 (G1865A)
cos1-86 (Q765->stop)
cos1-22 (G1721A)
mcl1+
cos1-7 (G724A)
mcl1-101 (C1477T, C2287T, G2303A)
mcl1Δ
sensitive to trichostatin Amcl1Δ44
sensitive to UV during vegetative growthmcl1-101 (C1477T, C2287T, G2303A)152
sensitive to valproic acidmcl1Δ155
viable vegetative cell populationcos1-86 (Q765->stop)3844
cos1-7 (G724A)
cos1-38 (G1865A)
mcl1Δ
cos1-22 (G1721A)
cos1-17 (T698A)

Cell Phenotype

Term NameGenotypesCount
abnormal ascospore wall biogenesismcl1Δ18
abnormal CENP-A containing chromatin organizationmcl1-101 (C1477T, C2287T, G2303A)15
abnormal chromosome morphologymcl1-1 (ntG372A)25
abnormal microtubule cytoskeleton morphology during mitotic interphasemcl1Δ186
abnormal mitotic cell cyclemcl1Δ882
abnormal mitotic cell cycle arrest with replicated DNAmcl1-102 (Mcl1 amino acids 1-268 plus KPSLAMVIIVS at C-terminus)45
abolished protein localization to CENP-A containing chromatin1
affects localization of cnp1mcl1Δ
complete but unequal mitotic sister chromatid segregationmcl1-1 (ntG372A)35
cutmcl1-1 (ntG372A)128
decreased chromatin silencing at centromere central corecos1-86 (Q765->stop)9
cos1-22 (G1721A)
mcl1-101 (C1477T, C2287T, G2303A)
cos1-17 (T698A)
cos1-7 (G724A)
cos1-38 (G1865A)
decreased chromatin silencing at centromere outer repeatmcl1Δ48
cos1-86 (Q765->stop)
cos1-17 (T698A)
cos1-38 (G1865A)
mcl1-101 (C1477T, C2287T, G2303A)
cos1-7 (G724A)
cos1-22 (G1721A)
decreased mitotic centromeric sister chromatid cohesionmcl1-1 (ntG372A)8
decreased origin firing efficiencymcl1-1 (ntG372A)2
decreased protein localization to CENP-A containing chromatin3
affects localization of cnp1mcl1-101 (C1477T, C2287T, G2303A)
decreased spatial extent of CENP-A containing nucleosome assemblymcl1-101 (C1477T, C2287T, G2303A)3
elongated telomeresmcl1-1 (ntG372A)174
elongated vegetative cellmcl1+794
incomplete mitotic sister chromatid segregation, with chromatin bridgemcl1-1 (ntG372A)4
increased duration of mitotic S phasemcl1-1 (ntG372A)23
increased histone H4-K16 acetylation at centromere central coremcl1-101 (C1477T, C2287T, G2303A)2
increased histone H4-K5, H4-K8, H4-K12 and H4-K16 acetylation at centromere central coremcl1-101 (C1477T, C2287T, G2303A)2
increased minichromosome loss during vegetative growthmcl1-1 (ntG372A)68
increased number of double-strand break sites during vegetative growthmcl1-102 (Mcl1 amino acids 1-268 plus KPSLAMVIIVS at C-terminus)19
mcl1Δ
inviable after spore germination, multiple cell divisions, unequal chromosome segregationmcl1Δ4
inviable elongated mononucleate aseptate cellmcl1Δ44
mcl1-101 (C1477T, C2287T, G2303A)
mcl1-102 (Mcl1 amino acids 1-268 plus KPSLAMVIIVS at C-terminus)
lagging mitotic chromosomescos1-17 (T698A)40
cos1-22 (G1721A)
cos1-38 (G1865A)
cos1-86 (Q765->stop)
mcl1-1 (ntG372A)
cos1-7 (G724A)
large and small daughter nucleimcl1Δ19
mislocalized mitotic spindlemcl1-1 (ntG372A)1
monoseptate vegetative cell with binucleate and anucleate compartmentsmcl1-1 (ntG372A)31
normal chromatin silencing at telomeremcl1Δ16
normal conjugation frequencymcl1Δ15
normal protein binding77
affecting pol1 and mcl1mcl1ΔN (1-419)
spores sensitive to acetonemcl1Δ3
unequal mitotic sister chromatid segregationcos1-38 (G1865A)63
cos1-7 (G724A)
cos1-22 (G1721A)
cos1-17 (T698A)
cos1-86 (Q765->stop)
viable branched, elongated vegetative cellmcl1Δ20
viable elongated vegetative cellmcl1Δ320
viable vegetative cell with abnormal cell shapemcl1Δ702

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased vegetative cell population growthwee-115, mcl1-1 (ntG372A)
mis16-myc (wild type Mis16 plus C-terminal Myc tag), mcl1-101 (C1477T, C2287T, G2303A)
rad1Δ, mcl1-1 (ntG372A)
mis6-302 (G135E), mcl1-101 (C1477T, C2287T, G2303A)
mcl1-1 (ntG372A), cds1Δ
bub1Δ, mcl1+
rad9Δ, mcl1-1 (ntG372A)
cds1Δ, mcl1-1 (ntG372A)
mcl1-1 (ntG372A), rad9Δ
mcl1-1 (ntG372A), bub1Δ
chk1Δ, cds1Δ, mcl1-1 (ntG372A)
inviable vegetative cell populationrad51Δ, mcl1-1 (ntG372A)
mcl1-1 (ntG372A), rqh1Δ
cds1-KD, mcl1-1 (ntG372A)
mcl1+, rad26Δ
cds1+, mcl1-1 (ntG372A)
mcl1-1 (ntG372A), rad54Δ
rqh1-K457I (K457I), mcl1-1 (ntG372A)
cdc24-M38 (R370->stop), mcl1-1 (ntG372A)
mcl1-101 (C1477T, C2287T, G2303A), ams2Δ
mcl1+, rad3Δ
rad3Δ, mcl1-1 (ntG372A)
hsk1-1312, mcl1-1 (ntG372A)
mcl1-101 (C1477T, C2287T, G2303A), cnp1-FH (wild type Cnp1 plus C-terminal 3 x FLAG and 6 x His)
rad21-K1, mcl1-1 (ntG372A)
rad26Δ, mcl1-1 (ntG372A)
mcl1+, rqh1Δ
loss of viability at high temperaturemcl1+, bub1Δ
cdc25-22 (C532Y), mcl1-1 (ntG372A)
mcl1-1 (ntG372A), bub1Δ
wee-115, mcl1-1 (ntG372A)
normal growth on methyl methanesulfonatedna2+, mcl1-1 (ntG372A)
normal growth on thiabendazolecds1Δ, mcl1-1 (ntG372A)
sensitive to ionizing radiation during vegetative growthchk1Δ, cds1Δ, mcl1-1 (ntG372A)
cds1Δ, mcl1-1 (ntG372A)
sensitive to thiabendazolesir2+, mcl1-101 (C1477T, C2287T, G2303A), clr3+
mcl1-101 (C1477T, C2287T, G2303A), clr3+

Cell Phenotype

Term NameGenotypes
abnormal sporulation resulting in formation of ascus with more than four sporeshsk1-1312, mcl1-1 (ntG372A)
decreased chromatin silencing at centromere outer repeatmcl1-101 (C1477T, C2287T, G2303A), clr3+
mcl1-101 (C1477T, C2287T, G2303A), sir2+
sir2+, mcl1-101 (C1477T, C2287T, G2303A), clr3+
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in cds1 replication checkpoint kinase Cds1
FYPO affected by mutation in hsk1 Dbf4(Dfp1)-dependent protein kinase Hsk1
FYPO affected by mutation in pof3 F-box protein Pof3
FYPO localization affected by mutation in cds1 replication checkpoint kinase Cds1
FYPO localization affected by mutation in hsk1 Dbf4(Dfp1)-dependent protein kinase Hsk1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3285778..3285554, 3285508..3285357, 3285298..3283004
Intron3285553..3285509, 3285356..3285299
mRNA3285778..3283004
5' UTR3285778..3285746PMID:21511999
CDS3285745..3285554, 3285508..3285357, 3285298..3283195
3' UTR3283194..3283004PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF12341 Pfam IPR022100 Minichromosome loss protein Mcl1, middle region 415 704 1
SM00320 SMART IPR001680 WD40 repeat 126 165 112
SM00320 SMART IPR001680 WD40 repeat 217 258 112
SM00320 SMART IPR001680 WD40 repeat 3 41 112
SM00320 SMART IPR001680 WD40 repeat 44 81 112
SM00320 SMART IPR001680 WD40 repeat 175 214 112
SM00320 SMART IPR001680 WD40 repeat 84 123 112
PS50082 Prosite Profiles IPR001680 WD40 repeat 226 267 100
PS50294 Prosite Profiles IPR017986 WD40-repeat-containing domain 9 267 104
PTHR19932 HMMPANTHER 1 812 1
2.130.10.10 Gene3D IPR015943 WD40/YVTN repeat-like-containing domain 10 294 145
SSF50978 SuperFamily IPR017986 WD40-repeat-containing domain 10 294 135

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000190WD repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000190

Protein Properties

Ave. residue weight 111.64 Da
Charge -21.50
Codon Adaptation Index 0.40
Isoelectric point 4.96
Molecular weight 90.99 kDa
Number of residues 815
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for mcl1 (SPAPB1E7.02c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5376during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5075during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4606during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4646during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4733during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
183.3during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.52during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAPB1E7.02c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
interacts withmcl1DNA polymerase alpha accessory factor Mcl1 PCAPMID:26771498
affinity capturespol1DNA polymerase alpha catalytic subunit Affinity Capture-WesternPMID:15643072
affinity capturespof3F-box protein Pof3 Affinity Capture-WesternPMID:16997270
forms complex withpol1DNA polymerase alpha catalytic subunit Reconstituted ComplexPMID:15643072
binds activation domain construct withmcl1DNA polymerase alpha accessory factor Mcl1 Two-hybridPMID:26771498
binds activation domain construct withlsm4U6 snRNP-associated protein Lsm4 (predicted) Two-hybridPMID:26771498
binds activation domain construct withpol1DNA polymerase alpha catalytic subunit Two-hybridPMID:15915339
binds DNA-binding domain construct withpof3F-box protein Pof3 Two-hybridPMID:16997270
binds DNA-binding domain construct withpol1DNA polymerase alpha catalytic subunit Two-hybridPMID:15915339
affinity captured bypof3F-box protein Pof3 Affinity Capture-WesternPMID:16997270
affinity captured bypol1DNA polymerase alpha catalytic subunit Affinity Capture-WesternPMID:15915339
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAPB1E7.02c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withmre11Mre11 nuclease Negative GeneticPMID:18818364
negative genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withraf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
negative genetic interaction withdcc1Ctf18 RFC-like complex subunit Dcc1 Negative GeneticPMID:18818364
negative genetic interaction withcsn71COP9/signalosome complex subunit 7a (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsts5RNB-like protein Negative GeneticPMID:22681890
negative genetic interaction withnot3CCR4-Not complex subunit Not3/5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withdbl8tRNA-splicing endonuclease positive effector Dbl8 Negative GeneticPMID:22681890
negative genetic interaction withhhf2histone H4 h4.2 Negative GeneticPMID:22681890
negative genetic interaction withtif38translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
negative genetic interaction withtrm10tRNA m(1)G methyltransferase Trm10 Negative GeneticPMID:22681890
negative genetic interaction withask1DASH complex subunit Ask1 Negative GeneticPMID:22681890
negative genetic interaction withSPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:18818364
negative genetic interaction withrdp1RNA-directed RNA polymerase Rdp1 Negative GeneticPMID:18818364
negative genetic interaction withrec8meiotic cohesin complex subunit Rec8 Negative GeneticPMID:22681890
negative genetic interaction withhhp2serine/threonine protein kinase Hhp2 Negative GeneticPMID:22681890
negative genetic interaction withmgr2TIM23 translocase complex subunit Mgr2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withdef1RNAPII degradation factor Def1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withchp1chromodomain protein Chp1 Negative GeneticPMID:18818364
negative genetic interaction withrik1silencing protein Rik1 Negative GeneticPMID:22681890
negative genetic interaction withatd1aldehyde dehydrogenase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
negative genetic interaction withsdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:18818364
negative genetic interaction withmcs6TFIIH associated cyclin-dependent protein kinase Mcs6 Negative GeneticPMID:22681890
negative genetic interaction withdad4DASH complex subunit Dad4 Negative GeneticPMID:22681890
negative genetic interaction withphp5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
negative genetic interaction witharp6actin-like protein Arp6 Negative GeneticPMID:18818364
negative genetic interaction withmik1mitotic inhibitor kinase Mik1 Negative GeneticPMID:22681890
negative genetic interaction withhht1histone H3 h3.1 Negative GeneticPMID:22681890
negative genetic interaction withnto1histone acetyltransferase complex subunit Nto1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withswd2Set1C complex subunit Swd2.1 Negative GeneticPMID:18818364
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
negative genetic interaction withsvf1Svf1 family protein Svf1 Negative GeneticPMID:22681890
negative genetic interaction withdnm1mitochondrial dynamin family GTPase Dnm1 Negative GeneticPMID:22681890
negative genetic interaction withswd3WD repeat protein Swd3 Negative GeneticPMID:18818364
negative genetic interaction withirc3ATP helicase Irc3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhmo1HMG box protein Hmo1 Negative GeneticPMID:18818364
negative genetic interaction withdad3DASH complex subunit Dad3 Negative GeneticPMID:22681890
negative genetic interaction withppk26protein kinase like PAN complex subunit Ppk26 Negative GeneticPMID:22681890
negative genetic interaction withppk27serine/threonine protein kinase Ppk27 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtel1ATM checkpoint kinase Negative GeneticPMID:22681890
negative genetic interaction withSPBC15C4.06cubiquitin-protein ligase E3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPCC1442.02central kinetochore associated family protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgar1snoRNP pseudouridylase box H/ACA snoRNP complex protein Gar1 Negative GeneticPMID:22681890
negative genetic interaction withnup60nucleoporin Nup60 Negative GeneticPMID:22681890
negative genetic interaction withrad1checkpoint clamp complex protein Rad1 Negative GeneticPMID:22681890
negative genetic interaction withhrq1RecQ type DNA helicase Hrq1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withutp16U3 snoRNP-associated protein Utp16 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcaf1CCR4-Not complex CAF1 family ribonuclease subunit Caf1 Negative GeneticPMID:18818364
negative genetic interaction withbzz1diacylglycerol binding protein Bzz1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withngg1SAGA complex subunit Ngg1 Negative GeneticPMID:18818364
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:18818364
negative genetic interaction withpof3F-box protein Pof3 Negative GeneticPMID:22681890
negative genetic interaction withhip1hira protein, histone chaperone Hip1 Negative GeneticPMID:22681890
negative genetic interaction withrad17RFC related checkpoint protein Rad17 Negative GeneticPMID:22681890
negative genetic interaction withSPBC1348.03S. pombe specific 5Tm protein family Negative GeneticPMID:22681890
negative genetic interaction withklp5kinesin-like protein Klp5 Negative GeneticPMID:22681890
negative genetic interaction withklp6kinesin-like protein Klp6 Negative GeneticPMID:22681890
negative genetic interaction withset11ribosomal protein lysine methyltransferase Set11 Negative GeneticPMID:22681890
negative genetic interaction withset1histone lysine methyltransferase Set1 Negative GeneticPMID:18818364
negative genetic interaction withhhf1histone H4 h4.1 Negative GeneticPMID:22681890
negative genetic interaction withpmc2mediator complex subunit Med1 Negative GeneticPMID:18818364
negative genetic interaction withspc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
negative genetic interaction withatp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withrad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Negative GeneticPMID:22681890
negative genetic interaction withrtt109RTT109 family histone lysine acetyltransferase Negative GeneticPMID:18818364
negative genetic interaction withfar10SIP/FAR complex FHA domain subunit Far10/Csc1 Negative GeneticPMID:22681890
negative genetic interaction withpar1protein phosphatase PP2A regulatory subunit B-56 Par1 Negative GeneticPMID:22681890
negative genetic interaction withSPBC29A10.16ccytochrome b5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhif2Set3 complex subunit Hif2 Negative GeneticPMID:22681890
negative genetic interaction withssb3DNA replication factor A subunit Ssb3 Negative GeneticPMID:22681890
negative genetic interaction withrad9checkpoint clamp complex protein Rad9 Negative GeneticPMID:18818364
negative genetic interaction withskp1SCF ubiquitin ligase complex subunit Skp1 Negative GeneticPMID:22681890
negative genetic interaction withrtn1reticulon-like protein Rtn1 Negative GeneticPMID:22681890
negative genetic interaction withrsc1RSC complex subunit Rsc1 Negative GeneticPMID:18818364
negative genetic interaction withmrc1mediator of replication checkpoint 1 Negative GeneticPMID:18818364
negative genetic interaction withgmh5alpha-1,2-galactosyltransferase (predicted) Negative GeneticPMID:18818364
negative genetic interaction withsty1MAP kinase Sty1 Negative GeneticPMID:22681890
negative genetic interaction withswr1SNF2 family ATP-dependent DNA helicase Swr1 Negative GeneticPMID:18818364
negative genetic interaction withmok11alpha-1,3-glucan synthase Mok11 Negative GeneticPMID:22681890
negative genetic interaction withsap14U2 snRNP-associated protein SF3B14 Sap14 Negative GeneticPMID:22681890
negative genetic interaction withcwf21complexed with Cdc5 protein Cwf21 Negative GeneticPMID:18818364
negative genetic interaction withhus1checkpoint clamp complex protein Hus1 Negative GeneticPMID:18818364
negative genetic interaction withssp2AMP-activated protein serine/threonine kinase alpha subunit Ssp2 Negative GeneticPMID:22681890
negative genetic interaction withpli1SUMO E3 ligase Pli1 Negative GeneticPMID:22681890
negative genetic interaction withswc2Swr1 complex complex subunit Swc2 Negative GeneticPMID:22681890
negative genetic interaction withrem1meiosis-specific cyclin Rem1 Negative GeneticPMID:22681890
negative genetic interaction withptn1phosphatidylinositol-3,4,5-trisphosphate3-phosphatase Ptn1 Negative GeneticPMID:22681890
negative genetic interaction withdcr1dicer Negative GeneticPMID:18818364
negative genetic interaction withcsn3COP9/signalosome complex subunit Csn3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcsn4COP9/signalosome complex subunit Csn4 Negative GeneticPMID:22681890
negative genetic interaction withtbf1DNA binding factor Trf1 Negative GeneticPMID:22681890
negative genetic interaction withmms19Dos2 silencing complex subunit Mms19 Negative GeneticPMID:18818364
negative genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
negative genetic interaction withrhp14XP-A family homolog Rhp14 Negative GeneticPMID:22681890
negative genetic interaction withhrd1synviolin family ubiquitin-protein ligase E3, Hrd1 Negative GeneticPMID:22681890
negative genetic interaction withsfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsir2Sirtuin family histone deacetylase Sir2 Negative GeneticPMID:22681890
negative genetic interaction withpub1HECT-type ubiquitin-protein ligase E3 Pub1 Negative GeneticPMID:22681890
negative genetic interaction withmed14mediator complex subunit Med14 Negative GeneticPMID:22681890
negative genetic interaction withswi6HP1 family chromodomain protein Swi6 Negative GeneticPMID:22681890
negative genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
negative genetic interaction withmsc1Swr1 complex subunit Chk1 Negative GeneticPMID:18818364
negative genetic interaction withhsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Negative GeneticPMID:22681890
negative genetic interaction withtrt1telomerase reverse transcriptase 1 protein Trt1 Negative GeneticPMID:22681890
negative genetic interaction withmga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Negative GeneticPMID:18818364
negative genetic interaction withmfm1M-factor precursor Mfm1 Negative GeneticPMID:22681890
negative genetic interaction withers1RNA-silencing factor Ers1 Negative GeneticPMID:22681890
negative genetic interaction withdpb4DNA polymerase epsilon subunit Dpb4 Negative GeneticPMID:22681890
negative genetic interaction withapc10anaphase-promoting complex substrate recognition subunit Apc10 Negative GeneticPMID:22681890
negative genetic interaction witharb1argonaute inhibitor protein 1 Negative GeneticPMID:22681890
negative genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:18818364
negative genetic interaction withspf1Set1C PHD Finger protein Spf1 Negative GeneticPMID:18818364
negative genetic interaction withSPBC36B7.08cnucleosome assembly protein (predicted) Negative GeneticPMID:18818364
negative genetic interaction withppa2serine/threonine protein phosphatase Ppa2 Negative GeneticPMID:22681890
negative genetic interaction withash2Ash2-trithorax family protein Negative GeneticPMID:18818364
negative genetic interaction withsgf29SAGA complex subunit Sgf29 Negative GeneticPMID:18818364
negative genetic interaction withark1aurora-B kinase Ark1 Negative GeneticPMID:22681890
negative genetic interaction withamk2AMP-activated protein kinase beta subunit Amk2 Negative GeneticPMID:18818364
negative genetic interaction withmad2mitotic spindle checkpoint protein Mad2 Negative GeneticPMID:22681890
negative genetic interaction withtas3RITS complex subunit 3 Negative GeneticPMID:22681890
negative genetic interaction withcrb2DNA repair protein Rad9 homolog Crb2 Negative GeneticPMID:18818364
negative genetic interaction withbdc1bromodomain containing protein 1, Bdc1 Negative GeneticPMID:18818364
negative genetic interaction withrpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:22681890
negative genetic interaction withvps71Swr1 complex subunit Vps71 Negative GeneticPMID:18818364
negative genetic interaction withmph1dual specificity protein kinase Mph1 Negative GeneticPMID:22681890
negative genetic interaction withhrp1ATP-dependent DNA helicase Hrp1 Negative GeneticPMID:22681890
negative genetic interaction withpds5mitotic and meiotic cohesin-associated protein Pds5 Negative GeneticPMID:18818364
negative genetic interaction withsap10splicing factor 3B Negative GeneticPMID:22681890
negative genetic interaction withrad3ATR checkpoint kinase Rad3 Negative GeneticPMID:22681890
negative genetic interaction withcdc7serine/threonine protein kinase Cdc7 Negative GeneticPMID:22681890
negative genetic interaction withSPBC1778.03cNADH pyrophosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcsn5COP9/signalosome complex subunit Csn5 Negative GeneticPMID:22681890
negative genetic interaction withswi3replication fork protection complex subunit Swi3 Negative GeneticPMID:18818364
negative genetic interaction withrad55RecA family ATPase Rad55/Rhp55 Negative GeneticPMID:22681890
negative genetic interaction withswc5Swr1 complex subunit Swc5 Negative GeneticPMID:18818364
negative genetic interaction withSPAC2F3.11exopolyphosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrad51RecA family recombinase Rad51/Rhp51 Negative GeneticPMID:18818364
negative genetic interaction withSPBC839.03cneddylation protein Dcn1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpus4tRNA pseudouridine synthase Pus4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC7D4.05hydrolase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmug154conserved fungal protein Negative GeneticPMID:22681890
negative genetic interaction withbdf2BET family double bromodomain protein Bdf2 Negative GeneticPMID:18818364
negative genetic interaction withrad57RecA family ATPase Rad57/Rhp57 Negative GeneticPMID:18818364
negative genetic interaction withmms1E3 ubiquitin ligase complex subunit Mms1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withbdf1Swr1 complex bromodomain subunit Bdf1 Negative GeneticPMID:22681890
negative genetic interaction withcsn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:22681890
negative genetic interaction withrrg9mitochondrial genome maintenance protein Rrg9 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtfs1transcription elongation factor TFIIS Negative GeneticPMID:22681890
negative genetic interaction withpht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:22681890
negative genetic interaction withgcn20AAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC144.17c6-phosphofructo-2-kinase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:18818364
negative genetic interaction withraf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Negative GeneticPMID:22681890
negative genetic interaction withrnc1RNA-binding protein that suppresses calcineurin deletion Rnc1 Negative GeneticPMID:22681890
negative genetic interaction withcph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
negative genetic interaction withbqt3bouquet formation protein Bqt3 Negative GeneticPMID:22681890
negative genetic interaction withrxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
negative genetic interaction withspc34DASH complex subunit Spc34 Negative GeneticPMID:22681890
negative genetic interaction withmto1MT organizer Mto1 Negative GeneticPMID:22681890
negative genetic interaction withalp14TOG/XMAP14 family protein Alp14 Negative GeneticPMID:18818364
negative genetic interaction withpcf2CAF assembly factor (CAF-1) complex subunit B, Pcf2 Negative GeneticPMID:22681890
negative genetic interaction withcdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
negative genetic interaction withpms1MutL family mismatch-repair protein Pms1 Negative GeneticPMID:22681890
negative genetic interaction withmhf2kinetochore protein, CENP-X ortholog, FANCM-MHF complex subunit Mhf2 Negative GeneticPMID:22681890
negative genetic interaction withphp3CCAAT-binding factor complex subunit Php3 Negative GeneticPMID:22681890
negative genetic interaction witheif21translation initiation factor eIF2A (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpzh1serine/threonine protein phosphatase Pzh1 Negative GeneticPMID:22681890
negative genetic interaction withctf18RFC-like complex subunit Ctf18 Negative GeneticPMID:22681890
negative genetic interaction withpob3FACT complex subunit Pob3 Negative GeneticPMID:18818364
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withtor1serine/threonine protein kinase Tor1 Negative GeneticPMID:22681890
negative genetic interaction withsrb11cyclin CycC, Srb mediator subunit Srb11 Negative GeneticPMID:18818364
negative genetic interaction withdbl2meiotic chromosome segregation protein Dbl2 Negative GeneticPMID:22681890
negative genetic interaction withbio2biotin synthase Negative GeneticPMID:22681890
negative genetic interaction withyaf9YEATS family histone acetyltransferase subunit Yaf9 Negative GeneticPMID:18818364
negative genetic interaction withypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
negative genetic interaction witharp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:22681890
negative genetic interaction withtos4FHA domain protein Tos4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withzds1zds family protein phosphatase type A regulator Zds1 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withcut2securin, sister chromatid separation inhibitor Negative GeneticPMID:22681890
negative genetic interaction withsnt2Lid2 complex subunit Snt2 Negative GeneticPMID:22681890
negative genetic interaction withswd1Set1C complex subunit Swd1 Negative GeneticPMID:18818364
negative genetic interaction withppk21serine/threonine protein kinase Ppk21 Negative GeneticPMID:22681890
negative genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
negative genetic interaction withsey1GTP binding protein Sey1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrpl160360S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsrp1SR family protein, human SRFS2 ortholog Srp1 Negative GeneticPMID:22681890
negative genetic interaction withsrs2ATP-dependent DNA helicase, UvrD subfamily Negative GeneticPMID:18818364
negative genetic interaction withcdm1DNA polymerase delta subunit Cdm1 Negative GeneticPMID:22681890
negative genetic interaction withSPAC732.02cfructose-2,6-bisphosphate 2-phosphatase activity (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpsl1cyclin pho85 family Psl1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withduo1DASH complex subunit Duo1 Negative GeneticPMID:22681890
positive genetic interaction withatg6autophagy associated beclin family protein Atg6 Positive GeneticPMID:22681890
positive genetic interaction withmtr4ATP-dependent RNA helicase, TRAMP complex subunit Mtr4 Positive GeneticPMID:22681890
positive genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
positive genetic interaction withSPBC1683.06curidine ribohydrolase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withpex11peroxisomal biogenesis factor 11 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withlsg1Lsk1 complex gamma subunit Lsg1 Positive GeneticPMID:22681890
positive genetic interaction withcsk1cyclin-dependent kinase/ cyclin-dependent kinase activating kinase Csk1 Positive GeneticPMID:22681890
positive genetic interaction withlcl3mitochondrial endonuclease, possibly DNA damage related (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPAC26F1.08cconserved protein Positive GeneticPMID:22681890
positive genetic interaction withdbr1RNA lariat debranching enzyme Dbr1 Positive GeneticPMID:22681890
positive genetic interaction withgrx3monothiol glutaredoxin Grx3 Positive GeneticPMID:22681890
positive genetic interaction withbmt2rRNA (adenine) methyltransferase activity Bmt2 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Positive GeneticPMID:18818364
positive genetic interaction withSPCC777.04amino acid transmembrane transporter (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPCC757.12alpha-amylase homolog (predicted) Positive GeneticPMID:22681890
positive genetic interaction withcgi121EKC/KEOPS complex subunit Cgi121 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withmlo2ubiquitin protein ligase E3 component human N-recognin 7 homolog Mlo2 Positive GeneticPMID:18818364
positive genetic interaction withSPAC18G6.13Schizosaccharomyces specific protein Positive GeneticPMID:22681890
positive genetic interaction withfbp1fructose-1,6-bisphosphatase Fbp1 Positive GeneticPMID:22681890
positive genetic interaction withhop1linear element associated protein Hop1 Positive GeneticPMID:18818364
positive genetic interaction withptc2protein phosphatase 2C Ptc2 Positive GeneticPMID:22681890
positive genetic interaction withrcd1RNA-binding protein, CCR4-NOT complex subunit Rcd1 Positive GeneticPMID:18818364
positive genetic interaction withnud3nuclear distribution protein NUDC homolog Positive GeneticPMID:22681890
positive genetic interaction withSPBC16G5.06Schizosaccharomyces specific protein Positive GeneticPMID:22681890
positive genetic interaction withmeu7alpha-amylase homolog Aah4 Positive GeneticPMID:22681890
positive genetic interaction withtif452translation initiation factor eIF4E, 4F complex E subunit isoform 2, stress response factor Positive GeneticPMID:22681890
positive genetic interaction withSPBC336.05csmall RNA 2'-O-methyltransferase activity (predicted) Positive GeneticPMID:18818364
positive genetic interaction withubp3ubiquitin C-terminal hydrolase Ubp3 Positive GeneticPMID:22681890
positive genetic interaction withSPBPB2B2.18Schizosaccharomyces specific protein Positive GeneticPMID:22681890
positive genetic interaction withSPCC576.02hydantoin racemase family (predicted) Positive GeneticPMID:22681890
positive genetic interaction withphf2Lsd1/2 complex PHD finger containing protein Phf2 Positive GeneticPMID:22681890
positive genetic interaction withcar1arginase Car1 Positive GeneticPMID:22681890
positive genetic interaction withSPCC736.13short chain dehydrogenase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withmug27meiosis specific protein kinase Mug27/Slk1 Positive GeneticPMID:22681890
positive genetic interaction withpuf3pumilio family RNA-binding protein Puf3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withpoz1Pot1 associated protein Poz1 Positive GeneticPMID:22681890
positive genetic interaction withSPCC70.03cproline dehydrogenase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withppk8serine/threonine protein kinase Ppk8 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withgnr1heterotrimeric G protein beta subunit Gnr1 Positive GeneticPMID:18818364
positive genetic interaction withSPCC4B3.13MatE family transmembrane transporter (predicted) Positive GeneticPMID:22681890
positive genetic interaction withtma20RNA-binding protein Tma20 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withjmj1histone demethylase Jmj1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withmit1SHREC complex ATP-dependent DNA helicase subunit Mit1 Positive GeneticPMID:22681890
positive genetic interaction withpil2meiotic eisosome BAR domain protein Pil2 Positive GeneticPMID:22681890
positive genetic interaction withSPBC1703.11optic atrophy 3 family protein Positive GeneticPMID:18818364
positive genetic interaction withnrl1spliceosome-associated protein Nrl1 Positive GeneticPMID:22681890
positive genetic interaction withpmc3mediator complex subunit Med27 Positive GeneticPMID:18818364
positive genetic interaction withprp4serine/threonine protein kinase Prp4 Positive GeneticPMID:22681890
positive genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsrp2splicing factor Srp2 Positive GeneticPMID:22681890
positive genetic interaction withdsc5UBX domain containing protein required for Sre1 cleavage Positive GeneticPMID:22681890
positive genetic interaction withbob1prefoldin subunit 5 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withvps38phophatidylinositol 3-kinase complex subunit Vps38 Positive GeneticPMID:22681890
synthetic growth defect withcdc22ribonucleoside reductase large subunit Cdc22 Synthetic Growth DefectPMID:12455694
synthetic growth defect withrhp18Rad18 homolog ubiquitin protein ligase E3, Rhp18 Synthetic Growth DefectPMID:12455694
synthetic growth defect withrad2FEN-1 endonuclease Rad2 Synthetic Growth DefectPMID:15915339
synthetic growth defect withrad1checkpoint clamp complex protein Rad1 Synthetic Growth DefectPMID:12455694
synthetic growth defect withmik1mitotic inhibitor kinase Mik1 Synthetic Growth DefectPMID:12455694
synthetic growth defect withbub1mitotic spindle checkpoint kinase Bub1 Synthetic Growth DefectPMID:12455694
synthetic growth defect withpol1DNA polymerase alpha catalytic subunit Synthetic Growth DefectPMID:12455694
synthetic growth defect withrad9checkpoint clamp complex protein Rad9 Synthetic Growth DefectPMID:12455694
synthetic growth defect withwee1M phase inhibitor protein kinase Wee1 Synthetic Growth DefectPMID:12455694
synthetic lethal withpol1DNA polymerase alpha catalytic subunit Synthetic LethalityPMID:15915339
synthetic lethal withdna2DNA replication endonuclease-helicase Dna2 Synthetic LethalityPMID:15915339
synthetic lethal withrad51RecA family recombinase Rad51/Rhp51 Synthetic LethalityPMID:15915339
synthetic lethal withrqh1RecQ type DNA helicase Rqh1 Synthetic LethalityPMID:15643072
synthetic lethal withpof3F-box protein Pof3 Synthetic LethalityPMID:16997270
synthetic lethal withhsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Synthetic LethalityPMID:15643072
synthetic lethal withrad3ATR checkpoint kinase Rad3 Synthetic LethalityPMID:12455694
synthetic lethal withrad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Synthetic LethalityPMID:12455694
synthetic lethal withrad21mitotic cohesin complex, non-SMC subunit Rad21 (kleisin) Synthetic LethalityPMID:12455694
synthetic lethal withcdc24DNA replication protein Cdc24 Synthetic LethalityPMID:12455694
synthetic lethal withcnp1centromere-specific histone H3 CENP-A Synthetic LethalityPMID:18493607
synthetic lethal withams2cell cycle regulated GATA-type transcription factor Ams2 Synthetic LethalityPMID:18493607
synthetically rescued bycds1replication checkpoint kinase Cds1 Synthetic RescuePMID:12455694
rescued by overexpression ofdna2DNA replication endonuclease-helicase Dna2 Dosage RescuePMID:12455694
rescued by overexpression ofsir2Sirtuin family histone deacetylase Sir2 Dosage RescuePMID:18493607
rescued by overexpression ofclr3histone deacetylase (class II) Clr3 Dosage RescuePMID:18493607
rescued bytop2DNA topoisomerase II Phenotypic SuppressionPMID:25669599
External References
Database Identifier Description
NBRP SPAPB1E7.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAPB1E7.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAPB1E7.02c BioGRID Interaction Datasets
Expression Viewer SPAPB1E7.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAPB1E7.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAPB1E7.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAPB1E7.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAPB1E7.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAPB1E7.02c Transcriptome Viewer (Bähler Lab)
GEO SPAPB1E7.02c GEO profiles
PInt SPAPB1E7.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAPB1E7.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAPB1E7.02c Fission yeast phenotypic data & analysis
Cyclebase SPAPB1E7.02c.1 Cell Cycle Data
UniProtKB/SwissProtQ9C107Minichromosome loss protein 1
ModBaseQ9C107Database of comparative protein structure models
STRINGQ9C107Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594128DNA polymerase alpha accessory factor Mcl1
RefSeq mRNANM_001019552972h- DNA polymerase alpha accessory factor Mcl1 (mcl1), mRNA
European Nucleotide ArchiveCAC36919.1ENA Protein Mapping
UniParcUPI000006C769UniProt Archive

Literature for mcl1

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016