dop1 (SPAPB21F2.02)


Gene Standard Namedop1 Characterisation Statusbiological role inferred
Systematic IDSPAPB21F2.02 Feature Typeprotein coding
Synonyms Name Description
ProductDopey family protein Dop1 Product Size1687aa, 192.65 kDa
Genomic Location Chromosome I, 492335-497927 (5593nt); CDS:492461-497689 (5229nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
endoplasmic reticulum organization17
Annotation ExtensionEvidenceWith/FromReference
Golgi to endosome transport12
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4212
Annotation ExtensionEvidenceWith/FromReference
Golgi apparatus357
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationdop1Δ3844

Cell Phenotype

Term NameGenotypesCount
elongated telomeresdop1Δ174
viable vegetative cell with normal cell morphologydop1Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons492335..492476, 492512..492630, 492673..494385, 494423..494615, 494667..497927
Intron492477..492511, 492631..492672, 494386..494422, 494616..494666
mRNA492335..497927
5' UTR492335..492460PMID:21511999
CDS492461..492476, 492512..492630, 492673..494385, 494423..494615, 494667..497689
3' UTR497690..497927PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04118 Pfam IPR007249 Dopey, N-terminal 10 302 1
PTHR14042 HMMPANTHER 1 1664 1
PTHR14042:SF24 HMMPANTHER 1 1664 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.20 Da
Charge -12.50
Codon Adaptation Index 0.39
Isoelectric point 5.85
Molecular weight 192.65 kDa
Number of residues 1687
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1677
present during mitotic M phaseS1594
S1594
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S1594 PMID:21712547
experimental evidence S1594 PMID:24763107
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for dop1 (SPAPB21F2.02)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1117during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1207during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1354during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1229during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2782.49during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1241during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3825.26during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.67during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAPB21F2.02 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
negative genetic interaction withpph3serine/threonine protein phosphatase, PP4 complex subunit Pph3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc4 Negative GeneticPMID:21504829
negative genetic interaction withypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
negative genetic interaction withsyj1inositol-polyphosphate 5-phosphatase (synaptojanin homolog 1) Negative GeneticPMID:22681890
negative genetic interaction withdmc1RecA family ATPase Dmc1 Negative GeneticPMID:22681890
negative genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
negative genetic interaction withsrp1SR family protein, human SRFS2 ortholog Srp1 Negative GeneticPMID:22681890
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withSPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
negative genetic interaction withprp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
negative genetic interaction withgma12alpha-1,2-galactosyltransferase Gma12 Negative GeneticPMID:22681890
negative genetic interaction withago1argonaute Negative GeneticPMID:22681890
negative genetic interaction withssu72phosphoric ester hydrolase Ssu72 (predicted) Negative GeneticPMID:22681890
negative genetic interaction witheif21translation initiation factor eIF2A (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
negative genetic interaction withclr1SHREC complex subunit Clr1 Negative GeneticPMID:22681890
negative genetic interaction withamk2AMP-activated protein kinase beta subunit Amk2 Negative GeneticPMID:22681890
negative genetic interaction withvps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
positive genetic interaction withhrp3ATP-dependent DNA helicase Hrp3 Positive GeneticPMID:22681890
positive genetic interaction withalp31tubulin specific chaperone cofactor A, Alp31 Positive GeneticPMID:22681890
positive genetic interaction withcdm1DNA polymerase delta subunit Cdm1 Positive GeneticPMID:22681890
positive genetic interaction withdsk1SR protein-specific kinase Dsk1 Positive GeneticPMID:22681890
positive genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Positive GeneticPMID:22681890
positive genetic interaction withies2Ino80 complex subunit Ies2 Positive GeneticPMID:22681890
positive genetic interaction withgcn20AAA family ATPase Gcn20 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withelp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withswc5Swr1 complex subunit Swc5 Positive GeneticPMID:22681890
positive genetic interaction withelp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAPB21F2.02 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAPB21F2.02 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAPB21F2.02 BioGRID Interaction Datasets
Expression Viewer SPAPB21F2.02 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAPB21F2.02 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAPB21F2.02 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAPB21F2.02 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAPB21F2.02 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAPB21F2.02 Transcriptome Viewer (Bähler Lab)
GEO SPAPB21F2.02 GEO profiles
PInt SPAPB21F2.02 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAPB21F2.02 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAPB21F2.02 Fission yeast phenotypic data & analysis
Cyclebase SPAPB21F2.02.1 Cell Cycle Data
UniProtKB/SwissProtQ9C0Z4Protein dopey
ModBaseQ9C0Z4Database of comparative protein structure models
STRINGQ9C0Z4Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592953Dopey family protein (predicted)
RefSeq mRNANM_001018353972h- Dopey family protein (predicted) (SPAPB21F2.02), mRNA
European Nucleotide ArchiveCAC36891.1ENA Protein Mapping
UniParcUPI000006B59FUniProt Archive

Literature for dop1

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016