SPAPB21F2.02


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAPB21F2.02 Feature Typeprotein coding
Synonyms Name Description
ProductDopey family protein (predicted) Product Size1687aa, 192.65 kDa
Genomic Location Chromosome I, 492335-497927 (5593nt); CDS:492461-497689 (5229nt)

Ensembl Gene Location
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006895Golgi to endosome transportISOSGD:S000002548GO_REF:000002410
GO:0007029endoplasmic reticulum organizationISOSGD:S000002548GO_REF:000002414
GO:0008360regulation of cell shapeISOSGD:S000002548GO_REF:000002482
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005794Golgi apparatusIEAUniProtKB-KW:KW-0333GO_REF:0000037355
GO:0005737cytoplasmISOSGD:S000002548GO_REF:00000244205
GO:0016020membraneIEAUniProtKB-KW:KW-0472GO_REF:00000371454
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopySPAPB21F2.02ΔNullPECO:0000005, PECO:0000137PMID:236978063759
MicroscopySPAPB21F2.02ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002019elongated telomeres158
expressivity FYPO_EXT:0000002Gel electrophoresis evidenceSPAPB21F2.02ΔNullPMID:20625380
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001MicroscopySPAPB21F2.02ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1492335492476
2492512492630
3492673494385
4494423494615
5494667497927

UTRs

Region Coordinates Reference
five_prime_UTR492335..492460PMID:21511999
three_prime_UTR497690..497927PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04118 Pfam IPR007249 Dopey, N-terminal 9 306 1
PTHR14042:SF14 HMMPANTHER 1 1683 1
PTHR14042 HMMPANTHER 1 1683 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.20 Da
Charge -12.50
Isoelectric point 5.85
Molecular weight 192.65 kDa
Number of residues 1687
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS1594PMID:247631071670
present during mitotic M phaseexperimental evidenceS1594PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1117during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1207during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1354during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1229during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1241during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2782.49during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
3825.26during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.67during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ase1antiparallel microtubule cross-linking factor Ase1 Positive GeneticPMID:22681890
pph3serine/threonine protein phosphatase, PP4 complex subunit Pph3 (predicted) Negative GeneticPMID:22681890
prp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
ago1argonaute Negative GeneticPMID:22681890
srp1SR family protein, human SRFS2 ortholog Srp1 Negative GeneticPMID:22681890
alp31tubulin specific chaperone cofactor A, Alp31 Positive GeneticPMID:22681890
elp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:22681890
cdm1DNA polymerase delta subunit Cdm1 Positive GeneticPMID:22681890
SPBC4B4.04translation initiation factor eIF2A (predicted) Negative GeneticPMID:22681890
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
SPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:22681890
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
SPBC29A3.09cAAA family ATPase Gcn20 (predicted) Positive GeneticPMID:22681890
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
clr1cryptic loci regulator Clr1 Negative GeneticPMID:22681890
dsk1SR protein-specific kinase Dsk1 Positive GeneticPMID:22681890
syj1inositol-polyphosphate 5-phosphatase (synaptojanin homolog 1) Negative GeneticPMID:22681890
dmc1RecA family ATPase Dmc1 Negative GeneticPMID:22681890
swc5Swr1 complex subunit Swc5 Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
ypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
gma12alpha-1,2-galactosyltransferase Gma12 Negative GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
hrp3ATP-dependent DNA helicase Hrp3 Positive GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
ies2Ino80 complex subunit Ies2 Positive GeneticPMID:22681890
amk2AMP-activated protein kinase beta subunit Amk2 Negative GeneticPMID:22681890
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
ssu72phosphoric ester hydrolase Ssu72 (predicted) Negative GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
vps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
elp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAPB21F2.02 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAPB21F2.02 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAPB21F2.02 BioGRID Interaction Datasets
Expression Viewer SPAPB21F2.02 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAPB21F2.02 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAPB21F2.02 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAPB21F2.02 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAPB21F2.02 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAPB21F2.02 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAPB21F2.02 Cell Cycle Data
GEO SPAPB21F2.02 GEO profiles
PInt SPAPB21F2.02 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAPB21F2.02 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAPB21F2.02 Fission yeast phenotypic data & analysis
UniProtKB/SwissProtQ9C0Z4Protein dopey
ModBaseQ9C0Z4Database of comparative protein structure models
STRINGQ9C0Z4Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592953Dopey family protein (predicted)
RefSeq mRNANM_001018353972h- Dopey family protein (predicted) (SPAPB21F2.02), mRNA
European Nucleotide ArchiveCAC36891.1ENA Protein Mapping
UniParcUPI000006B59FUniProt Archive

Literature for SPAPB21F2.02

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014