SPAPB2B4.04c


Gene Standard NameUnassigned Characterisation Statuspublished
Systematic IDSPAPB2B4.04c Feature Typeprotein coding
Synonymspmc1 Name Description
Productvacuolar calcium transporting P-type ATPase P2 type, Pmc1 Product Size1292aa, 142.24 kDa
Genomic Location Chromosome I, 2718444-2712704 (5741nt); CDS:2716931-2713053 (3879nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037557
GO:0005509calcium ion bindingISMIPR006408GO_REF:000000152
GO:0005388calcium-transporting ATPase activityISOSGD:S000002974GO_REF:00000246
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0070588calcium ion transmembrane transportISOSGD:S000002974GO_REF:000002411
GO:0006816calcium ion transportISOSGD:S000002974GO_REF:000002414
GO:0006874cellular calcium ion homeostasisIMPPMID:1692895915
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0071627integral component of fungal-type vacuolar membraneIDAPMID:154702406
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001020normal growth on calcium ionCell growth assaySPAPB2B4.04cΔNullPECO:0000114PMID:154702404
FYPO:0001420normal vegetative cell population growth rateCell growth assaySPAPB2B4.04cΔNullPECO:0000080, PECO:0000005PMID:1916898841
FYPO:0000098sensitive to calcium8
expressivity FYPO_EXT:0000003Cell growth assaySPAPB2B4.04cΔNullPECO:0000005PMID:19168988
Cell growth assaySPAPB2B4.04cΔNullPECO:0000126PMID:15470240
Cell growth assaySPAPB2B4.04cΔNullPECO:0000137PMID:15470240
FYPO:0002060viable vegetative cell populationCell growth assaySPAPB2B4.04cΔNullPECO:0000137PMID:154702403730
MicroscopySPAPB2B4.04cΔNullPMID:20473289
MicroscopySPAPB2B4.04cΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001MicroscopySPAPB2B4.04cΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
127184442712704

UTRs

Region Coordinates Reference
five_prime_UTR2718444..2716932PMID:21511999
three_prime_UTR2713052..2712704PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00690 Pfam IPR004014 Cation-transporting P-type ATPase, N-terminal 207 248 6
PF00689 Pfam IPR006068 Cation-transporting P-type ATPase, C-terminal 966 1137 3
PF00702 Pfam IPR023214 HAD-like domain 541 893 9
PF00122 Pfam IPR008250 P-type ATPase, A domain 282 535 14
SM00831 SMART IPR004014 Cation-transporting P-type ATPase, N-terminal 140 253 5
TMhelix TMHMM 1118 1136 959
TMhelix TMHMM 1018 1040 959
TMhelix TMHMM 1086 1108 959
TMhelix TMHMM 237 259 959
TMhelix TMHMM 490 512 959
TMhelix TMHMM 939 961 959
TMhelix TMHMM 448 470 959
TMhelix TMHMM 274 296 959
PS00154 Prosite Patterns IPR018303 P-type ATPase, phosphorylation site 545 551 14
PTHR24093 HMMPANTHER 38 1152 9
PTHR24093:SF254 HMMPANTHER 38 1152 1
1.20.1110.10 Gene3D IPR023298 P-type ATPase, transmembrane domain 858 1143 5
1.20.1110.10 Gene3D IPR023298 P-type ATPase, transmembrane domain 354 431 5
3.40.1110.10 Gene3D IPR023299 P-type ATPase, cytoplasmic domain N 533 784 13
0049473 SuperFamily 144 170 14
0049473 SuperFamily 929 1144 14
0049473 SuperFamily 212 313 14
0049473 SuperFamily 429 537 14
SSF81660 SuperFamily IPR023299 P-type ATPase, cytoplasmic domain N 552 784 11
0049471 SuperFamily 315 424 14
SSF56784 SuperFamily IPR023214 HAD-like domain 542 561 41
SSF56784 SuperFamily IPR023214 HAD-like domain 720 986 41
PR00119 PRINTS IPR001757 Cation-transporting P-type ATPase 879 898 14
PR00119 PRINTS IPR001757 Cation-transporting P-type ATPase 903 915 14
PR00119 PRINTS IPR001757 Cation-transporting P-type ATPase 361 375 14
PR00119 PRINTS IPR001757 Cation-transporting P-type ATPase 543 557 14
PR00119 PRINTS IPR001757 Cation-transporting P-type ATPase 777 788 14
PR00119 PRINTS IPR001757 Cation-transporting P-type ATPase 799 809 14
TIGR01517 tigrfam IPR006408 Calcium-transporting P-type ATPase, subfamily IIB 605 1141 1
TIGR01494 tigrfam IPR001757 Cation-transporting P-type ATPase 846 961 14
TIGR01494 tigrfam IPR001757 Cation-transporting P-type ATPase 479 559 14

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 110.09 Da
Charge -1.00
Isoelectric point 6.46
Molecular weight 142.24 kDa
Number of residues 1292
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS193PMID:217125471669
present during mitotic M phase
present during mitotic M phaseS18PMID:21712547
present during mitotic M phaseS15PMID:21712547
S193PMID:24763107
MOD:00696phosphorylated residueNASPMID:195477441921
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased in presence of purvalanol A expression microarray evidencePMID:22840777

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
3128during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
3504during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
3227during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
3582during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1686.07during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
3595during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1597.92during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.2during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
1.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
deafness3
Warning
DescriptionQualifierReferenceCount
gene name conflict19
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
elp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:22681890
vps35retromer complex subunit Vps35 Positive GeneticPMID:22681890
plb1phospholipase B homolog Plb1 Negative GeneticPMID:22681890
set1histone lysine methyltransferase Set1 Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Positive GeneticPMID:22681890
pmr1P-type ATPase, calcium transporting Pmr1 Dosage RescuePMID:15470240
Synthetic Lethality
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
hus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
ask1DASH complex subunit Ask1 Negative GeneticPMID:22681890
par1protein phosphatase regulatory subunit Par1 Positive GeneticPMID:22681890
spc34DASH complex subunit Spc34 Negative GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Positive GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
jmj2histone demethylase Jmj2 Positive GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Positive GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
crp79poly(A) binding protein Crp79 Affinity Capture-RNAPMID:20531409
External References
Database Identifier Description
NBRP SPAPB2B4.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAPB2B4.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAPB2B4.04c BioGRID Interaction Datasets
Expression Viewer SPAPB2B4.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAPB2B4.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAPB2B4.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAPB2B4.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAPB2B4.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAPB2B4.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAPB2B4.04c Cell Cycle Data
GEO SPAPB2B4.04c GEO profiles
PInt SPAPB2B4.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAPB2B4.04c Peptides identified in tandem mass spectrometry proteomics experiments
IntEnz3.6.3.8Integrated relational Enzyme database
Rhea3.6.3.8Annotated reactions database
WikiGene2543477vacuolar calcium transporting P-type ATPase, Pmc1
EntrezGene2543477vacuolar calcium transporting P-type ATPase, Pmc1
UniProtKB/SwissProtQ9HDW7Calcium-transporting ATPase 2
ModBaseQ9HDW7Database of comparative protein structure models
STRINGQ9HDW7Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593890vacuolar calcium transporting P-type ATPase, Pmc1
RefSeq mRNANM_001019320972h- vacuolar calcium transporting P-type ATPase, Pmc1 (SPAPB2B4.04c), mRNA
European Nucleotide ArchiveBAA87254ENA Protein Mapping
European Nucleotide ArchiveBAA87254.1ENA Protein Mapping
European Nucleotide ArchiveCAC21470ENA Protein Mapping
European Nucleotide ArchiveCAC21470.1ENA Protein Mapping
UniParcUPI000006AEE7UniProt Archive

Literature for SPAPB2B4.04c

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014