lsb4 (SPAPJ696.02)


Gene Standard Namelsb4 Characterisation Statusbiological role inferred
Systematic IDSPAPJ696.02 Feature Typeprotein coding
Synonyms Name Description
Productactin cortical patch component Lsb4 (predicted) Product Size430aa, 46.37 kDa
Genomic Location Chromosome I, 724145-725946 (1802nt); CDS:724289-725720 (1432nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
actin filament organization53
Annotation ExtensionEvidenceWith/FromReference
endocytosis76
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cortical patch47
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationlsb4Δ3840

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologylsb4Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons724145..724325, 724378..724418, 724506..725946
Intron724326..724377, 724419..724505
mRNA724145..725946
5' UTR724145..724288PMID:21511999
CDS724289..724325, 724378..724418, 724506..725720
3' UTR725721..725946PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04366 Pfam IPR007461 Ysc84 actin-binding domain 89 212 1
PF00018 Pfam IPR001452 SH3 domain 377 424 20
SM00326 SMART IPR001452 SH3 domain 374 430 21
PS50002 Prosite Profiles IPR001452 SH3 domain 371 430 21
PTHR15629:SF2 HMMPANTHER 1 429 1
PTHR15629 HMMPANTHER 1 429 1
2.30.30.40 Gene3D Spectrin repeat 368 429 21
SSF50044 SuperFamily IPR001452 SH3 domain 368 429 21
PR00452 PRINTS IPR001452 SH3 domain 374 384 12
PR00452 PRINTS IPR001452 SH3 domain 418 430 12
PR00452 PRINTS IPR001452 SH3 domain 407 416 12
PR00452 PRINTS IPR001452 SH3 domain 388 403 12

View domain organization at Pfam

Protein Properties

Ave. residue weight 107.85 Da
Charge 7.50
Codon Adaptation Index 0.43
Isoelectric point 9.51
Molecular weight 46.37 kDa
Number of residues 430
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during cellular response to thiabendazoleS285
present during cellular response to thiabendazoleS430
present during mitotic M phaseS300
present during cellular response to thiabendazoleS318
S357
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S285 PMID:18257517
present during cellular response to thiabendazole IDA S430 PMID:18257517
present during mitotic M phase experimental evidence S300 PMID:21712547
present during cellular response to thiabendazole IDA S318 PMID:18257517
experimental evidence S357 PMID:24763107
O-phospho-L-threonine 701
present during cellular response to thiabendazoleT316
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA T316 PMID:18257517
phosphorylated residue 1931
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysineK367 512
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence K367 PMID:26412298
IDA PMID:26412298
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for lsb4 (SPAPJ696.02)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
16234during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
17414during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
13949during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
16816during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5867.13during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
18346during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8017.66during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.7during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2506
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAPJ696.02 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byepe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
External References
Database Identifier Description
NBRP SPAPJ696.02 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAPJ696.02 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAPJ696.02 BioGRID Interaction Datasets
Expression Viewer SPAPJ696.02 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAPJ696.02 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAPJ696.02 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAPJ696.02 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAPJ696.02 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAPJ696.02 Transcriptome Viewer (Bähler Lab)
GEO SPAPJ696.02 GEO profiles
PInt SPAPJ696.02 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAPJ696.02 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAPJ696.02 Fission yeast phenotypic data & analysis
Cyclebase SPAPJ696.02.1 Cell Cycle Data
UniProtKB/SwissProtQ9URW6SH3 domain-containing protein PJ696.02
ModBaseQ9URW6Database of comparative protein structure models
STRINGQ9URW6Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593048actin cortical patch component Lsb4 (predicted)
RefSeq mRNANM_001018447972h- actin cortical patch component Lsb4 (predicted) (SPAPJ696.02), mRNA

Literature for lsb4

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016