cut4 (SPBC106.09)


Gene Standard Namecut4 Characterisation Statuspublished
Systematic IDSPBC106.09 Feature Typeprotein coding
Synonymsapc1 Name Description
Productanaphase-promoting complex subunit Apc1 Product Size1458aa, 165.41 kDa
Genomic Location Chromosome II, 390925-396037 (5113nt); CDS:390989-395365 (4377nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0061630ubiquitin protein ligase activity71
contributes_toICGO:0005680GO_REF:0000001
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0031145anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic processICGO:0005680GO_REF:000000114
GO:0008054cyclin catabolic processICGO:0005680PMID:891888011
GO:0016567protein ubiquitinationICGO:0005680GO_REF:0000001108
GO:0030071regulation of mitotic metaphase/anaphase transitionICGO:0005680PMID:891888049
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005680anaphase-promoting complexIDAPMID:891888013
GO:0005829cytosolIDAPMID:168233722319
GO:0005634nucleusIDAPMID:168233722737
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopycut4ΔNullPMID:204732891337
Microscopycut4ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopycut4ΔNullPMID:12618370

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopycut4ΔNullPECO:0000004, PECO:0000137PMID:23697806640
FYPO:0003165cutMicroscopynot recorded (unrecorded)PMID:891888080
FYPO:0001490inviable elongated vegetative cell389
penetrance FYPO_EXT:0000003Microscopycut4ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0001511inviable vegetative cell, abnormal cell shape, normal cell size199
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000001
Microscopycut4ΔNullPECO:0000137, PECO:0000005PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1390925396037
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF12859 Pfam IPR024990 Anaphase-promoting complex subunit 1 67 141 1
PTHR12827:SF4 HMMPANTHER 66 1457 1
PTHR12827 HMMPANTHER IPR024990 Anaphase-promoting complex subunit 1 66 1457 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.45 Da
Charge 4.00
Isoelectric point 6.69
Molecular weight 165.41 kDa
Number of residues 1458
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
183.73during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.77during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.34during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
sus1SAGA complex subunit Sus1 Synthetic RescuePMID:24948786
apc15anaphase-promoting complex subunit Apc15 Synthetic LethalityPMID:12477395
cut2securin, sister chromatid separation inhibitor Synthetic LethalityPMID:12653962
cut23anaphase-promoting complex subunit Apc8 Synthetic Growth DefectPMID:18354085
Dosage RescuePMID:15507118
cgs2cAMP-specific phosphodiesterase Cgs2 Dosage RescuePMID:8918880
cut8tethering factor for nuclear proteasome Cut8 Synthetic LethalityPMID:11084332
ubp8SAGA complex ubiquitin C-terminal hydrolase Ubp8 Synthetic RescuePMID:24948786
slp1sleepy homolog Slp1 Synthetic RescuePMID:9461438
mad2spindle checkpoint protein Mad2 Synthetic RescuePMID:9461438
rpn1219S proteasome regulatory subunit Rpn12 Synthetic Growth DefectPMID:16904908
cgs1cAMP-dependent protein kinase regulatory subunit Cgs1 Dosage RescuePMID:8918880
sti1chaperone activator Sti1 (predicted) Dosage RescuePMID:8918880
clr6histone deacetylase (class I) Clr6 Synthetic RescuePMID:18354085
sgf11SAGA complex subunit Sgf11 Synthetic RescuePMID:24948786
cut1separase/separin Synthetic LethalityPMID:12653962
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
apc13anaphase-promoting complex subunit Apc13 Affinity Capture-MSPMID:12477395
mad3mitotic spindle checkpoint protein Mad3 Affinity Capture-MSPMID:18556659
cut2securin, sister chromatid separation inhibitor Biochemical ActivityPMID:21389117
rpn1019S proteasome regulatory subunit Rpn10 Reconstituted ComplexPMID:12615927
mes1meiosis II protein Mes1 Biochemical ActivityPMID:21389117
cdc13G2/M B-type cyclin Cdc13 Biochemical ActivityPMID:21389117
apc2anaphase-promoting complex cullin family subunit Apc2 Affinity Capture-WesternPMID:18354085
cut9anaphase-promoting complex subunit Cut9 Co-fractionationPMID:10526233
Affinity Capture-WesternPMID:8918880
Co-fractionation
cut20anaphase-promoting complex subunit Apc4 Affinity Capture-WesternPMID:10526233
Co-fractionation
Affinity Capture-MSPMID:16950791
Affinity Capture-MSPMID:18556659
Affinity Capture-MSPMID:18082611
Affinity Capture-MSPMID:12477395
rec10meiotic recombination protein Rec10 Affinity Capture-MSPMID:19756689
nuc2anaphase-promoting complex subunit Apc3 Co-fractionationPMID:10526233
Co-fractionationPMID:8918880
Affinity Capture-Western
External References
Database Identifier Description
NBRP SPBC106.09 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC106.09 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC106.09 BioGRID Interaction Datasets
Expression Viewer SPBC106.09 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC106.09 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC106.09 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC106.09 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC106.09 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC106.09 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC106.09 Cell Cycle Data
GEO SPBC106.09 GEO profiles
PInt SPBC106.09 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC106.09 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN48/48G04Orfeome Localization Data
UniProtKB/SwissProtQ9URV2Anaphase-promoting complex subunit 1
ModBaseQ9URV2Database of comparative protein structure models
STRINGQ9URV2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595158anaphase-promoting complex subunit Apc1
RefSeq mRNANM_001021067972h- anaphase-promoting complex subunit Apc1 (cut4), mRNA
European Nucleotide ArchiveBAA22618.1ENA Protein Mapping
European Nucleotide ArchiveCAB53725.1ENA Protein Mapping
UniParcUPI000012870EUniProt Archive

Literature for cut4

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014