cut4 (SPBC106.09)


Gene Standard Namecut4 Characterisation Statuspublished
Systematic IDSPBC106.09 Feature Typeprotein coding
Synonymsapc1 Name Description
Productanaphase-promoting complex, platform subcomplex scaffold subunit Apc1 Product Size1458aa, 165.41 kDa
Genomic Location Chromosome II, 390925-396037 (5113nt); CDS:390989-395365 (4377nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
positive regulation of mitotic metaphase/anaphase transition55
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
anaphase-promoting complex13
Annotation ExtensionEvidenceWith/FromReference
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
nucleus2695
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationcut4ΔNull1435

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclecut4ΔNull846
cutunnamed (unrecorded)93
inviable elongated vegetative cellcut4ΔNull408
inviable vegetative cell, abnormal cell shape, normal cell sizecut4ΔNull199
normal attachment of spindle microtubules to kinetochorecut4tsNot specified3
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons390925..396037
mRNA390925..396037
5' UTR390925..390988PMID:21511999
CDS390989..395365
3' UTR395366..396037PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF12859 Pfam IPR024990 Anaphase-promoting complex subunit 1 67 141 1
PTHR12827 HMMPANTHER IPR024990 Anaphase-promoting complex subunit 1 43 1452 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.45 Da
Charge 4.00
Isoelectric point 6.69
Molecular weight 165.41 kDa
Number of residues 1458
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
183.73during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.77during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.34during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byapc13anaphase-promoting complex TPR lobe accessory factor Apc13 Affinity Capture-MSPMID:12477395
affinity captured byapc2anaphase-promoting complex cullin family subunit Apc2 Affinity Capture-WesternPMID:18354085
affinity captured bycut20anaphase-promoting complex, platform subcomplex scaffold subunit Apc4/Cut20 Affinity Capture-WesternPMID:10526233
co-fractionates withcut20anaphase-promoting complex, platform subcomplex scaffold subunit Apc4/Cut20 Co-fractionationPMID:10526233
affinity captured bycut20anaphase-promoting complex, platform subcomplex scaffold subunit Apc4/Cut20 Affinity Capture-MSPMID:16950791
affinity captured bycut20anaphase-promoting complex, platform subcomplex scaffold subunit Apc4/Cut20 Affinity Capture-MSPMID:18556659
affinity captured bycut20anaphase-promoting complex, platform subcomplex scaffold subunit Apc4/Cut20 Affinity Capture-MSPMID:18082611
affinity captured bycut20anaphase-promoting complex, platform subcomplex scaffold subunit Apc4/Cut20 Affinity Capture-MSPMID:12477395
co-fractionates withcut9anaphase-promoting complex, TPR lobe subcomplex subunit Cut9/Apc6 Co-fractionationPMID:10526233
co-fractionates withcut9anaphase-promoting complex, TPR lobe subcomplex subunit Cut9/Apc6 Co-fractionationPMID:8918880
affinity capturescut9anaphase-promoting complex, TPR lobe subcomplex subunit Cut9/Apc6 Affinity Capture-WesternPMID:8918880
affinity captured bymad3mitotic spindle checkpoint protein Mad3 Affinity Capture-MSPMID:18556659
co-fractionates withnuc2anaphase-promoting complex TPR lobe subcomplex subunit Apc3 Co-fractionationPMID:10526233
co-fractionates withnuc2anaphase-promoting complex TPR lobe subcomplex subunit Apc3 Co-fractionationPMID:8918880
affinity capturesnuc2anaphase-promoting complex TPR lobe subcomplex subunit Apc3 Affinity Capture-WesternPMID:8918880
affinity captured byrec10meiotic recombination protein Rec10 Affinity Capture-MSPMID:19756689
forms complex withrpn1019S proteasome regulatory subunit Rpn10 Reconstituted ComplexPMID:12615927
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withapc15anaphase-promoting complex, platform subcomplex scaffold subunit Apc15 Synthetic LethalityPMID:12477395
rescued by overexpression ofcgs1cAMP-dependent protein kinase regulatory subunit Cgs1 Dosage RescuePMID:8918880
rescued by overexpression ofcgs2cAMP-specific phosphodiesterase Cgs2 Dosage RescuePMID:8918880
synthetically rescued byclr6histone deacetylase (class I) Clr6 Synthetic RescuePMID:18354085
synthetic lethal withcut1separase/separin Synthetic LethalityPMID:12653962
synthetic lethal withcut2securin, sister chromatid separation inhibitor Synthetic LethalityPMID:12653962
synthetic growth defect withcut23anaphase-promoting complex, TPR lobe subcomplex subunit Apc8 Synthetic Growth DefectPMID:18354085
overexpression rescuescut23anaphase-promoting complex, TPR lobe subcomplex subunit Apc8 Dosage RescuePMID:15507118
synthetic lethal withcut8tethering factor for nuclear proteasome Cut8 Synthetic LethalityPMID:11084332
synthetically rescued bymad2mitotic spindle checkpoint protein Mad2 Synthetic RescuePMID:9461438
synthetic growth defect withrpn1219S proteasome regulatory subunit Rpn12 Synthetic Growth DefectPMID:16904908
synthetically rescued bysgf11SAGA complex subunit Sgf11 Synthetic RescuePMID:24948786
synthetically rescued byslp1substrate-specific APC activator Slp1 Synthetic RescuePMID:9461438
rescued by overexpression ofsti1chaperone activator Sti1 (predicted) Dosage RescuePMID:8918880
synthetically rescued bysus1SAGA complex subunit Sus1 Synthetic RescuePMID:24948786
synthetically rescued byubp8SAGA complex ubiquitin C-terminal hydrolase Ubp8 Synthetic RescuePMID:24948786
External References
Database Identifier Description
NBRP SPBC106.09 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC106.09 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC106.09 BioGRID Interaction Datasets
Expression Viewer SPBC106.09 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC106.09 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC106.09 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC106.09 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC106.09 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC106.09 Transcriptome Viewer (Bähler Lab)
GEO SPBC106.09 GEO profiles
PInt SPBC106.09 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC106.09 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC106.09 Fission yeast phenotypic data & analysis
Cyclebase SPBC106.09.1 Cell Cycle Data
SPD / RIKEN48/48G04Orfeome Localization Data
UniProtKB/SwissProtQ9URV2Anaphase-promoting complex subunit 1
ModBaseQ9URV2Database of comparative protein structure models
STRINGQ9URV2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595158anaphase-promoting complex subunit Apc1
RefSeq mRNANM_001021067972h- anaphase-promoting complex subunit Apc1 (cut4), mRNA
European Nucleotide ArchiveBAA22618.1ENA Protein Mapping
European Nucleotide ArchiveCAB53725.1ENA Protein Mapping
UniParcUPI000012870EUniProt Archive

Literature for cut4

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015