cut4 (SPBC106.09)

Gene Standard Namecut4 Characterisation Statuspublished
Systematic IDSPBC106.09 Feature Typeprotein coding
Synonymsapc1 Name Description
Productanaphase-promoting complex, platform subcomplex scaffold subunit Apc1 Product Size1458aa, 165.41 kDa
Genomic Location Chromosome II, 390925-396037 (5113nt); CDS:390989-395365 (4377nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
proteasome binding3
Annotation ExtensionEvidenceWith/FromReference
ubiquitin protein ligase activity66
Annotation ExtensionEvidenceWith/FromReference
has substrate cdc13
GO Biological Process
Term NameCount
anaphase-promoting complex-dependent catabolic process15
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
anaphase-promoting complex13
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
nuclear pericentric heterochromatin35
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationcut4Δ1468
sensitive to cadmiumcut4-533321
sensitive to cobaltcut4-533115
sensitive to nickelcut4-5332
slow vegetative cell population growthsng2-1369
viable vegetative cell populationcut4-5333862

Cell Phenotype

Term NameGenotypesCount
abnormal cell cycle arrest in mitotic metaphasecut4-53320
abnormal chromatin silencing at centromere outer repeatsng2-158
abnormal chromatin silencing at rDNAcut4-5538
abnormal chromatin silencing at silent mating-type cassettesng2-135
abnormal mitotic cell cyclecut4Δ903
cut cell with decreased poly(A)+ mRNA export from nucleuscut4-12
decreased DNA double-strand break formation at mating-type locus7
affecting rad21cut4-553
decreased histone H3-K9 dimethylation at centromere during vegetative growthsng2-117
decreased histone H3-K9 dimethylation at silent mating-type cassette during vegetative growthsng2-17
decreased protein level at centromere outer repeat5
affecting swi6sng2-1
affecting rad21sng2-1
affecting rad21cut4-553
decreased protein level at mating type region3
affecting swi6sng2-1
delayed onset of protein degradation during mitosis5
affecting cdc13cut4-533
increased minichromosome loss upon segregation during vegetative growth24
affecting rad21sng2-1
affecting rad21cut4-553
increased protein level during vegetative growth121
affecting slp1cut4-553
inviable elongated vegetative cellcut4Δ440
inviable vegetative cell, abnormal cell shape, normal cell sizecut4Δ199
meiosis and sporulation in haploidsng2-110
normal attachment of spindle microtubules to kinetochorecut4ts3

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased vegetative cell population growthcut4-553, mal2-1 (E277K)
inviable vegetative cell populationcut4-533, rpn10Δ
slp1-362 (AA376->stop), cut4-553
cut8-563 (T601C), cut4-553
apc15Δ, cut4-553

Cell Phenotype

Term NameGenotypes
decreased protein level during vegetative growth
affecting cdc13mts2-1, cut4ts
Target Of
FYPO affected by mutation in swi6 HP1 family chromodomain protein Swi6
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR390925..390988PMID:21511999
3' UTR395366..396037PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PTHR12827 HMMPANTHER IPR024990 Anaphase-promoting complex subunit 1 66 1417 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.45 Da
Charge 4.00
Codon Adaptation Index 0.38
Isoelectric point 6.69
Molecular weight 165.41 kDa
Number of residues 1458

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS211, S213 2293
Annotation ExtensionEvidenceResidueReference
IDA S213 PMID:25720772
IDA S211 PMID:25720772
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for cut4 (SPBC106.09)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
183.73during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.77during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
0.34during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
predominantly single copy (one to one)3087
conserved in fungi4608
conserved in eukaryotes4516
conserved in metazoa3498
conserved in vertebrates3473
conserved in eukaryotes only2525

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC106.09 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
co-fractionates withcut9anaphase-promoting complex, TPR lobe subcomplex subunit Cut9/Apc6 Co-fractionationPMID:10526233
co-fractionates withnuc2anaphase-promoting complex TPR lobe subcomplex subunit Apc3 Co-fractionationPMID:8918880
co-fractionates withcut20anaphase-promoting complex, platform subcomplex scaffold subunit Apc4/Cut20 Co-fractionationPMID:10526233
affinity captured bycut20anaphase-promoting complex, platform subcomplex scaffold subunit Apc4/Cut20 Affinity Capture-MSPMID:12477395
affinity captured byrec10meiotic recombination protein Rec10 Affinity Capture-MSPMID:19756689
affinity captured byapc2anaphase-promoting complex cullin family subunit Apc2 Affinity Capture-WesternPMID:18354085
affinity captured byapc13anaphase-promoting complex TPR lobe accessory factor Apc13 Affinity Capture-MSPMID:12477395
affinity captured bymad3mitotic spindle checkpoint protein Mad3 Affinity Capture-MSPMID:18556659
affinity captured bycut9anaphase-promoting complex, TPR lobe subcomplex subunit Cut9/Apc6 Affinity Capture-MSPMID:15060174
affinity captured byswi6HP1 family chromodomain protein Swi6 Affinity Capture-WesternPMID:19117951
forms complex withrpn1019S proteasome regulatory subunit Rpn10 Reconstituted ComplexPMID:12615927
affinity capturesnuc2anaphase-promoting complex TPR lobe subcomplex subunit Apc3 Affinity Capture-WesternPMID:8918880
affinity capturescut9anaphase-promoting complex, TPR lobe subcomplex subunit Cut9/Apc6 Affinity Capture-WesternPMID:8918880
affinity capturesswi6HP1 family chromodomain protein Swi6 Affinity Capture-WesternPMID:19117951
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC106.09 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withmal2kinetochore protein, CENP-O ortholog Mal2 Synthetic Growth DefectPMID:12242294
synthetic growth defect withcut23anaphase-promoting complex, TPR lobe subcomplex subunit Apc8 Synthetic Growth DefectPMID:18354085
synthetic growth defect withrpn1219S proteasome regulatory subunit Rpn12 Synthetic Growth DefectPMID:16904908
rescued by overexpression ofcgs2cAMP-specific phosphodiesterase Cgs2 Dosage RescuePMID:8918880
rescued by overexpression ofsti1chaperone activator Sti1 (predicted) Dosage RescuePMID:8918880
rescued by overexpression ofcgs1cAMP-dependent protein kinase regulatory subunit Cgs1 Dosage RescuePMID:8918880
synthetically rescued bymad2mitotic spindle checkpoint protein Mad2 Synthetic RescuePMID:9461438
synthetically rescued byslp1substrate-specific mitotic metaphase/anaphase APC coactivator Slp1 Synthetic RescuePMID:9461438
synthetically rescued byclr6histone deacetylase (class I) Clr6 Synthetic RescuePMID:18354085
synthetically rescued bysgf11SAGA complex subunit Sgf11 Synthetic RescuePMID:24948786
synthetically rescued bysus1SAGA complex subunit Sus1 Synthetic RescuePMID:24948786
synthetically rescued byubp8SAGA complex ubiquitin C-terminal hydrolase Ubp8 Synthetic RescuePMID:24948786
synthetic lethal withapc15anaphase-promoting complex, platform subcomplex scaffold subunit Apc15 Synthetic LethalityPMID:12477395
synthetic lethal withcut2securin, sister chromatid separation inhibitor Synthetic LethalityPMID:12653962
synthetic lethal withcut8tethering factor for nuclear proteasome Cut8 Synthetic LethalityPMID:11084332
synthetic lethal withcut1separase/separin Synthetic LethalityPMID:12653962
overexpression rescuescut23anaphase-promoting complex, TPR lobe subcomplex subunit Apc8 Dosage RescuePMID:15507118
External References
Database Identifier Description
NBRP SPBC106.09 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC106.09 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC106.09 BioGRID Interaction Datasets
Expression Viewer SPBC106.09 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC106.09 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC106.09 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC106.09 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC106.09 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC106.09 Transcriptome Viewer (Bähler Lab)
GEO SPBC106.09 GEO profiles
PInt SPBC106.09 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC106.09 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC106.09 Fission yeast phenotypic data & analysis
Cyclebase SPBC106.09.1 Cell Cycle Data
SPD / RIKEN48/48G04Orfeome Localization Data
UniProtKB/SwissProtQ9URV2Anaphase-promoting complex subunit 1
ModBaseQ9URV2Database of comparative protein structure models
STRINGQ9URV2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595158anaphase-promoting complex subunit Apc1
RefSeq mRNANM_001021067972h- anaphase-promoting complex subunit Apc1 (cut4), mRNA
European Nucleotide ArchiveBAA22618.1ENA Protein Mapping
European Nucleotide ArchiveCAB53725.1ENA Protein Mapping
UniParcUPI000012870EUniProt Archive

Literature for cut4

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017