pka1 (SPBC106.10)


Gene Standard Namepka1 Characterisation Statuspublished
Systematic IDSPBC106.10 Feature Typeprotein coding
Synonymsgit6, sam1, sam5, sam6, sam7, tpk Name DescriptionGlucose Insensitive Transcription
ProductcAMP-dependent protein kinase catalytic subunit Pka1 Product Size512aa, 57.58 kDa
Genomic Location Chromosome II, 396752-398957 (2206nt); CDS:396992-398530 (1539nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding551
Annotation ExtensionEvidenceWith/FromReference
cAMP-dependent protein kinase activity1
Annotation ExtensionEvidenceWith/FromReference
protein binding900
protein serine/threonine kinase activity105
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cAMP-mediated signaling10
Annotation ExtensionEvidenceWith/FromReference
cellular age-dependent response to reactive oxygen species1
Annotation ExtensionEvidenceWith/FromReference
glucose mediated signaling pathway8
Annotation ExtensionEvidenceWith/FromReference
negative regulation of induction of conjugation with cellular fusion13
Annotation ExtensionEvidenceWith/FromReference
negative regulation of transcription by glucose11
Annotation ExtensionEvidenceWith/FromReference
signal transduction involved in regulation of aerobic respiration3
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased bipolar indexpka1Δ27
decreased cell population growth on glucose carbon sourcepka1Δ393
decreased cell population growth on glycerol carbon sourcepka1Δ80
decreased mating efficiencypka1Δ276
decreased septation indexpka1Δ23
decreased spore germination frequencypka1Δ70
decreased sporulation frequencyT356D (T356D)103
increased conjugation frequencysam5 (G441E)10
sam6 (W461->amber)
sam7 (G441R)
sam1 (G359D)
increased mating efficiencysam6 (W461->amber)36
pka1Δ
increased monopolar indexpka1Δ39
increased sporulation frequencysam7 (G441R)12
T356A (T356A)
sam5 (G441E)
pka1Δ
increased viability in stationary phasepka1Δ62
increased viability upon nitrogen starvationpka1Δ54
normal growth during cellular response to high osmolaritysam7 (G441R)5
sam5 (G441E)
sam6 (W461->amber)
sam1 (G359D)
normal growth during cellular response to UVsam5 (G441E)81
sam7 (G441R)
sam6 (W461->amber)
sam1 (G359D)
normal vegetative cell population growthsam6 (W461->amber)846
pka1Δ
normal viability in stationary phasepka1Δ34
resistance to heat shock during vegetative growthpka1Δ7
resistance to salt stressT356D (T356D)7
sensitive to bortezomibpka1Δ255
sensitive to caffeine during vegetative growthpka1Δ110
sensitive to calciumpka1Δ29
sensitive to camptothecinpka1Δ259
sensitive to clotrimazolepka1Δ108
sensitive to cobaltpka1Δ115
sensitive to hydrogen peroxidepka1Δ160
sensitive to micafunginpka1Δ119
sensitive to potassium chloridepka1Δ30
sensitive to rotenonepka1Δ2
sensitive to salt stressT356A (T356A)102
pka1Δ
sam7 (G441R)
sam6 (W461->amber)
sam1 (G359D)
sam5 (G441E)
sam5 (G441E)
sam7 (G441R)
sensitive to terbinafinepka1Δ103
slow cell population growth during recovery from stationary phasepka1Δ3
slow vegetative cell population growthpka1Δ369
viable vegetative cell populationpka1Δ3862

Cell Phenotype

Term NameGenotypesCount
abnormal microtubule cytoskeleton morphology during mitotic interphasepka1Δ187
abnormal negative regulation of transcription by glucose15
affecting fbp1git6-261
abnormal protein phosphorylation during vegetative growth175
affecting rst2sam7 (G441R)
affecting rst2sam5 (G441E)
affecting rst2pka1Δ
affecting pka1sam5 (G441E)
affecting pka1T356A (T356A)
affecting pka1sam7 (G441R)
affecting pka1T356D (T356D)
abnormal vegetative cell morphologysam6 (W461->amber)1173
abolished protein modification during vegetative growth91
affecting pka1pka1Δ
affecting pka1sam5 (G441E)
affecting pka1sam7 (G441R)
abolished protein-protein interaction164
affecting pka1T356D (T356D)
affecting pka1G441R (G441R)
affecting pka1G441E (G441E)
affecting pka1T356A (T356A)
abolished stress granule assemblypka1Δ1
decreased alpha,alpha-trehalase activity during cellular response to nutrientpka1Δ1
decreased negative regulation of transcription by glucosegit6-26111
decreased phosphatase activity during cellular response to adenine starvationpka1Δ8
decreased phosphatase activity during cellular response to phosphate starvationpka1Δ11
decreased protein phosphorylation during vegetative growth105
affecting cut9pka1Δ
affecting atf1pka1Δ
decreased repression of transcription during cellular response to cAMP1
affecting rst2pka1Δ
affecting SO:0001865pka1Δ
decreased RNA level during cellular response to hypoxia7
affecting mae2pka1 mutant
decreased RNA level during cellular response to phosphate starvation5
affecting pho1pka1Δ
decreased transcription during cellular response to calcium ion8
affecting SO:0001865pka1Δ
affecting SO:0001865pka1+
delayed onset of sporulationpka1Δ2
elongated cell during nitrogen starvationpka1+25
elongated multiseptate vegetative cellpka1Δ69
elongated vegetative cellpka1+810
increased cellular reactive oxygen species level during vegetative growthpka1Δ33
increased cellular respirationpka1Δ3
increased cellular trehalose level during heat shockpka1Δ3
increased cytoplasmic protein level during vegetative growth3
affecting pka1G441E (G441E)
affecting pka1G441R (G441R)
affecting pka1T356D (T356D)
increased nuclear protein level during vegetative growth13
affecting rst2pka1Δ
affecting pka1T356D (T356D)
affecting ste11sam7 (G441R)
affecting ste11sam5 (G441E)
increased oxygen consumption during vegetative growthpka1Δ3
increased protein level during vegetative growth121
affecting prz1pka1Δ
affecting atf1pka1Δ
increased protein localization to nucleus24
affects localization of ste11pka1Δ
increased protein phosphorylation during vegetative growth66
affecting sty1pka1Δ
increased pseudohyphal growth during cellular hyperosmotic responsepka1+1
increased RNA catabolic process during nitrogen starvationpka1Δ1
increased RNA level during nitrogen starvation22
affecting fbp1pka1Δ
increased RNA level during vegetative growth356
affecting tpp1pka1Δ
affecting pcr1pka1Δ
affecting prz1pka1Δ
affecting fbp1git6-261
affecting fbp1pka1Δ
affecting ste11pka1Δ
affecting gpx1pka1Δ
inviable elongated vegetative cellpka1+440
mitochondrial aggregation during cellular response to reactive oxygen speciespka1Δ1
normal alpha,alpha-trehalase activitypka1Δ6
normal alpha,alpha-trehalase activity during cellular response to nutrientpka1Δ1
normal alpha,alpha-trehalase activity increase during cellular response to heat stresspka1Δ3
normal mitochondrial morphologypka1Δ5
normal protein level during vegetative growth103
affecting pka1G441R (G441R)
affecting pka1G441E (G441E)
normal protein localization to nucleus during cellular response to calcium ion1
affecting prz1pka1Δ
normal protein localization to nucleus during vegetative growth85
affecting cgs1pka1Δ
normal protein serine phosphorylation during vegetative growth12
affecting gad8pka1Δ
normal RNA level during cellular response to phosphate starvation3
affecting pho84pka1Δ
normal spore germinationpka1Δ22
normal stress granule assemblypka1Δ3
normal transcription during glucose starvation2
affecting rst2pka1Δ
normal tRNA methyltransferase activity3
affecting pmt1pka1Δ
sterilepka1+61
viable elongated vegetative cellpka1+329
viable small vegetative cellpka1Δ56
viable spheroid vegetative cellpka1Δ53
viable vegetative cell with abnormal cell shapepka1Δ709

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
abnormal mitotic cell cycle arrest with unreplicated DNAtor2-51, pka1Δ
decreased cell population growth on glucose carbon sourcepka1Δ, psk1+
decreased cell population growth on glycerol carbon sourceprz1Δ, pka1Δ
decreased mating efficiencypka1Δ, pcr1Δ
decreased spore germination frequencysck1Δ, pka1Δ
increased mating efficiencytor2-51, pka1Δ
inviable vegetative cell populationpka1Δ, cig2+, pat1-114
loss of viability in stationary phasepka1Δ, sty1Δ
sensitive to calciumprz1Δ, pka1Δ
viable vegetative cell populationpat1-114, pka1+, nrd1Δ
pka1+, cig2Δ, pat1-114
pka1Δ, nrd1+, pat1-114

Cell Phenotype

Term NameGenotypes
abnormal negative regulation of transcription by glucose
affecting fbp1git6-261, cgs1-1
branched, elongated cellprz1+ (wild type), pka1+
increased RNA level
affecting ste11tor2-51, pka1Δ
affecting rum1tor2-51, pka1Δ
affecting rum1pka1Δ, rum1d3'1 (lacks AU rich elements in 3' UTR)
RNA absent from cell during nitrogen starvation
affecting ste11pka1Δ, pcr1Δ
viable small vegetative cellcbf11Δ, pka1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in pka1 cAMP-dependent protein kinase catalytic subunit Pka1
FYPO localization affected by mutation in cgs1 cAMP-dependent protein kinase regulatory subunit Cgs1
FYPO localization affected by mutation in cyr1 adenylate cyclase
GO regulated by cgs1 cAMP-dependent protein kinase regulatory subunit Cgs1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons396752..398957
mRNA396752..398957
5' UTR396752..396991SP08622
CDS396992..398530
3' UTR398531..398957SP08622
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 202 456 108
SM00133 SMART IPR000961 AGC-kinase, C-terminal 457 512 11
SM00220 SMART IPR000719 Protein kinase domain 201 456 107
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 320 332 91
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 207 230 70
PS50011 Prosite Profiles IPR000719 Protein kinase domain 201 456 108
PS51285 Prosite Profiles IPR000961 AGC-kinase, C-terminal 457 512 13
PTHR24353 HMMPANTHER 180 512 1
1.10.510.10 Gene3D Immunoglobulin-like fold 267 468 112
3.30.200.20 Gene3D Immunoglobulin-like fold 189 266 111
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 191 485 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.46 Da
Charge 7.50
Codon Adaptation Index 0.43
Isoelectric point 7.22
Molecular weight 57.58 kDa
Number of residues 512
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-threonine 1087
present during cellular response to thiabendazoleT356
T356
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA T356 PMID:18257517
IDA T356 PMID:25720772
phosphorylated residue 2515
added during cellular response to glucose starvation
T356
Annotation ExtensionEvidenceResidueReference
added during cellular response to glucose starvation IDA PMID:21869531
IDA T356 PMID:21879336
O-phosphorylated residueS350,S352, S350,T351, S350,T356, T351,S352, T351,T356 2461
Annotation ExtensionEvidenceResidueReference
IDA S350,S352 PMID:25720772
IDA S350,T351 PMID:25720772
IDA T351,T356 PMID:25720772
IDA S350,T356 PMID:25720772
IDA T351,S352 PMID:25720772
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelincreased during GO:0071277Western blot evidencePMID:27756188
RNA levelincreased during GO:0071470expression microarray evidencePMID:12529438
increased during GO:0034605expression microarray evidencePMID:12529438
increased in presence of cadmium sulfate expression microarray evidencePMID:12529438
increased during GO:0034599expression microarray evidencePMID:12529438
increased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438

Quantitative Gene Expression

View graphical display of gene expression data for pka1 (SPBC106.10)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8313during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
9647during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8233during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
9718during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
9283during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1805.1during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
4284.72during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.7during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.94during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
Pathway
DescriptionQualifierReferenceCount
PKA2
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4608
conserved in eukaryotes4516
conserved in metazoa3498
conserved in vertebrates3473
conserved in eukaryotes only2525
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC106.10 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesatf1transcription factor, Atf-CREB family Atf1 Affinity Capture-WesternPMID:23640764
affinity capturescgs1cAMP-dependent protein kinase regulatory subunit Cgs1 Affinity Capture-WesternPMID:21869531
affinity capturessds23PP2A-type phosphatase inhibitor Sds23/Moc1 Affinity Capture-WesternPMID:23640764
affinity captured bycgs1cAMP-dependent protein kinase regulatory subunit Cgs1 Affinity Capture-WesternPMID:21869531
modifiessro1stress responsive orphan 1 Biochemical ActivityPMID:18410345
modifiesatf1transcription factor, Atf-CREB family Atf1 Biochemical ActivityPMID:23640764
modifiessds23PP2A-type phosphatase inhibitor Sds23/Moc1 Biochemical ActivityPMID:23640764
binds DNA-binding domain construct withgim1prefoldin subunit 6, Gim1 (predicted) Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC106.10 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescuesaps1diadenosine 5',5'''-p1,p6-hexaphosphate hydrolase Aps1 Phenotypic SuppressionPMID:25547512
rescuescbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Phenotypic SuppressionPMID:26366556
rescuesrcd1CCR4-NOT complex RNA-binding protein subunit Rcd1 Phenotypic SuppressionPMID:9447985
rescuesado1adenosine kinase (predicted) Phenotypic SuppressionPMID:25547512
rescuescsk1cyclin-dependent kinase/ cyclin-dependent kinase activating kinase Csk1 Phenotypic SuppressionPMID:25547512
rescuespac2cAMP-independent regulatory protein Pac2 Phenotypic SuppressionPMID:8536311
rescuesSPBC713.07cvacuolar polyphosphatase (predicted) Phenotypic SuppressionPMID:21169418
rescued bysty1MAP kinase Sty1 Phenotypic SuppressionPMID:20075862
rescued bypac2cAMP-independent regulatory protein Pac2 Phenotypic SuppressionPMID:8536311
rescued byrcd1CCR4-NOT complex RNA-binding protein subunit Rcd1 Phenotypic SuppressionPMID:9447985
rescued bypcr1transcription factor Pcr1 Phenotypic SuppressionPMID:8552099
rescued bysck1serine/threonine protein kinase Sck1 Phenotypic SuppressionPMID:11004189
rescued bypyp1tyrosine phosphatase Pyp1 Phenotypic SuppressionPMID:8832414
rescued byphp5CCAAT-binding factor complex subunit Php5 Phenotypic SuppressionPMID:11238405
rescued bytps1alpha,alpha-trehalose-phosphate synthase [UDP-forming] Phenotypic SuppressionPMID:9302019
rescued bynrd1RNA-binding protein Nrd1 Phenotypic SuppressionPMID:15166138
rescued bypyp2tyrosine phosphatase Pyp2 Phenotypic SuppressionPMID:8832414
rescued bymsa1RNA-binding protein Msa1 Phenotypic SuppressionPMID:15166138
rescued byphx1stationary phase-specific homeobox transcription factor Phx1 Phenotypic SuppressionPMID:25102102
enhances phenotype ofcbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Phenotypic EnhancementPMID:26366556
enhances phenotype oftor2serine/threonine protein kinase Tor2 Phenotypic EnhancementPMID:20634885
enhances phenotype ofcdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Phenotypic EnhancementPMID:22624651
enhances phenotype ofsck1serine/threonine protein kinase Sck1 Phenotypic EnhancementPMID:7498728
enhances phenotype ofisp6vacuolar serine protease Isp6 Phenotypic EnhancementPMID:16550352
overexpression rescuespat1serine/threonine protein kinase Ran1/Pat1 Dosage RescuePMID:9671458
overexpression rescuesgit1C2 domain protein Git1 Dosage RescuePMID:16143612
overexpression rescuescyr1adenylate cyclase Dosage RescuePMID:16143612
phenotype enhanced bysck1serine/threonine protein kinase Sck1 Phenotypic EnhancementPMID:9245826
phenotype enhanced bysds23PP2A-type phosphatase inhibitor Sds23/Moc1 Phenotypic EnhancementPMID:23640764
phenotype enhanced bysck2serine/threonine protein kinase Sck2 Phenotypic EnhancementPMID:9560431
rescued by overexpression ofsck1serine/threonine protein kinase Sck1 Dosage RescuePMID:22976295
rescued by overexpression ofrad2514-3-3 protein Rad25 Dosage RescuePMID:19584544
rescued by overexpression ofsck2serine/threonine protein kinase Sck2 Dosage RescuePMID:22976295
rescued by overexpression ofrad2414-3-3 protein Rad24 Dosage RescuePMID:19584544
rescued by overexpression ofprz1calcineurin responsive transcription factor Prz1 Dosage RescuePMID:22496451
rescued by overexpression ofesc1transcription factor Esc1 (predicted) Dosage RescuePMID:16143612
rescued by overexpression ofgad8AGC family protein kinase Gad8 Dosage RescuePMID:24928510
synthetic growth defect withprz1calcineurin responsive transcription factor Prz1 Synthetic Growth DefectPMID:27756188
synthetic growth defect withwis1MAP kinase kinase Wis1 Synthetic Growth DefectPMID:23640764
synthetically rescuescut9anaphase-promoting complex, TPR lobe subcomplex subunit Cut9/Apc6 Synthetic RescuePMID:18354085
synthetically rescuescut1separase/separin Synthetic RescuePMID:16861909
synthetically rescueshtb1histone H2B Htb1 Synthetic RescuePMID:16688222
synthetically rescuesnup184nucleoporin Nup184 Synthetic RescuePMID:10388805
synthetically rescuescut2securin, sister chromatid separation inhibitor Synthetic RescuePMID:16861909
growth defect in presence of overexpressedsds23PP2A-type phosphatase inhibitor Sds23/Moc1 Dosage Growth DefectPMID:23640764
synthetic lethal withsty1MAP kinase Sty1 Synthetic LethalityPMID:20453258
inviable in presence of overexpressedsds23PP2A-type phosphatase inhibitor Sds23/Moc1 Dosage LethalityPMID:23640764
External References
Database Identifier Description
NBRP SPBC106.10 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC106.10 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC106.10 BioGRID Interaction Datasets
Expression Viewer SPBC106.10 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC106.10 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC106.10 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC106.10 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC106.10 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC106.10 Transcriptome Viewer (Bähler Lab)
GEO SPBC106.10 GEO profiles
PInt SPBC106.10 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC106.10 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC106.10 Fission yeast phenotypic data & analysis
Cyclebase SPBC106.10.1 Cell Cycle Data
SPD / RIKEN24/24H10Orfeome Localization Data
IntEnz2.7.11.1Integrated relational Enzyme database
Rhea2.7.11.1Annotated reactions database
UniProtKB/SwissProtP40376cAMP-dependent protein kinase catalytic subunit
ModBaseP40376Database of comparative protein structure models
STRINGP40376Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595159cAMP-dependent protein kinase catalytic subunit Pka1
RefSeq mRNANM_001021068972h- cAMP-dependent protein kinase catalytic subunit Pka1 (pka1), mRNA
European Nucleotide ArchiveU08622ENA EMBL mapping
European Nucleotide ArchiveBAA04891.1ENA Protein Mapping
European Nucleotide ArchiveCAB53726.1ENA Protein Mapping
UniParcUPI000012DC1CUniProt Archive

Literature for pka1

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017