pre6 (SPBC106.16)


Gene Standard Namepre6 Characterisation Statuspublished
Systematic IDSPBC106.16 Feature Typeprotein coding
Synonyms Name Description
Product20S proteasome complex subunit alpha 4 Pre6 Product Size259aa, 28.28 kDa
Genomic Location Chromosome II, 409158-410766 (1609nt); CDS:409496-410453 (958nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004175endopeptidase activity66
contributes_toISSUniProtKB:P40303GO_REF:0000001
GO:0004298threonine-type endopeptidase activityIEAUniProtKB-KW:KW-0888GO_REF:000003715
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0043161proteasome-mediated ubiquitin-dependent protein catabolic processISSUniProtKB:P40303GO_REF:0000001110
GO:0007346regulation of mitotic cell cycleNASGO_REF:0000051281
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722316
GO:0005634nucleusIDAPMID:168233722740
GO:0019773proteasome core complex, alpha-subunit complexIDAPMID:177616707
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001023normal growth on cisplatinCell growth assaypre6ΔNullPECO:0000137, PECO:0000103, PECO:0000005PMID:2124741636
FYPO:0002060viable vegetative cell populationMicroscopypre6ΔNullPMID:204732893760
Microscopypre6ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3086
penetrance FYPO_EXT:0000001Microscopypre6ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1409158409594
2409655410028
3410147410766

UTRs

Region Coordinates Reference
five_prime_UTR409158..409495PMID:21511999
three_prime_UTR410454..410766PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00227 Pfam IPR001353 Proteasome, subunit alpha/beta 27 210 14
PF10584 Pfam IPR000426 Proteasome alpha-subunit, N-terminal domain 4 26 7
SM00948 SMART IPR000426 Proteasome alpha-subunit, N-terminal domain 4 26 7
PS00388 Prosite Patterns IPR000426 Proteasome alpha-subunit, N-terminal domain 4 26 7
PS51475 Prosite Profiles IPR023332 Proteasome A-type subunit 19 229 7
PTHR11599 HMMPANTHER 2 257 14
PTHR11599:SF15 HMMPANTHER 2 257 1
3.60.20.10 Gene3D IPR029055 Nucleophile aminohydrolases, N-terminal 3 238 19
SSF56235 SuperFamily IPR029055 Nucleophile aminohydrolases, N-terminal 2 235 22

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.18 Da
Charge 2.00
Isoelectric point 7.57
Molecular weight 28.28 kDa
Number of residues 259
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAS12PMID:182575171663
present during cellular response to thiabendazole
MOD:00696phosphorylated residueNASPMID:182575171915
NASPMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
51105during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
53609during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
25962during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
20169.47during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
51617during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
49679during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
47724during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
8.8during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3393
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
bag102BAG family molecular chaperone regulator Bag102 Affinity Capture-WesternPMID:24497846
Reconstituted Complex
uch2ubiquitin C-terminal hydrolase Uch2 Affinity Capture-MSPMID:20838651
bag101BAG family molecular chaperone regulator Bag101 (predicted) Reconstituted ComplexPMID:24497846
Affinity Capture-Western
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
rpt119S proteasome regulatory subunit Rpt1 (predicted) Affinity Capture-MSPMID:22307589
rpn1119S proteasome regulatory subunit Rpn11 Affinity Capture-MSPMID:20838651
External References
Database Identifier Description
NBRP SPBC106.16 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC106.16 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC106.16 BioGRID Interaction Datasets
Expression Viewer SPBC106.16 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC106.16 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC106.16 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC106.16 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC106.16 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC106.16 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC106.16 Cell Cycle Data
GEO SPBC106.16 GEO profiles
PInt SPBC106.16 Protein-Protein Interaction Predictor (Bähler Lab)
IntEnz3.4.25.1Integrated relational Enzyme database
Rhea3.4.25.1Annotated reactions database
EntrezGene254017520S proteasome component alpha 4 Pre6
WikiGene254017520S proteasome component alpha 4 Pre6
SPD / RIKEN13/13E11Orfeome Localization Data
UniProtKB/SwissProtQ10329Probable proteasome subunit alpha type-4
ModBaseQ10329Database of comparative protein structure models
STRINGQ10329Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_59516520S proteasome component alpha 4 Pre6
RefSeq mRNANM_001021074972h- 20S proteasome component alpha 4 Pre6 (pre6), mRNA
MEROPST01.974
European Nucleotide ArchiveCAB53732ENA Protein Mapping
European Nucleotide ArchiveCAB53732.1ENA Protein Mapping
UniParcUPI000013246BUniProt Archive

Literature for pre6

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014