pre6 (SPBC106.16)

Gene Standard Namepre6 Characterisation Statuspublished
Systematic IDSPBC106.16 Feature Typeprotein coding
Synonyms Name Description
Product20S proteasome complex subunit alpha 4 Pre6 Product Size259aa, 28.28 kDa
Genomic Location Chromosome II, 409158-410766 (1609nt); CDS:409496-410453 (958nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
endopeptidase activity65
Annotation ExtensionEvidenceWith/FromReference
threonine-type endopeptidase activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
positive regulation of mitotic metaphase/anaphase transition51
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001023normal growth on cisplatinCell growth assaypre6ΔNullPECO:0000137, PECO:0000103, PECO:0000005PMID:2124741636
FYPO:0002060viable vegetative cell populationMicroscopypre6ΔNullPECO:0000005, PECO:0000137PMID:236978063759

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopypre6ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
GO transported by crm1 importin family nuclear export receptor Crm1 PMID:20133687
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF10584 Pfam IPR000426 Proteasome alpha-subunit, N-terminal domain 4 26 7
PF00227 Pfam IPR001353 Proteasome, subunit alpha/beta 27 210 14
SM00948 SMART IPR000426 Proteasome alpha-subunit, N-terminal domain 4 26 7
PS00388 Prosite Patterns IPR000426 Proteasome alpha-subunit, N-terminal domain 4 26 7
PS51475 Prosite Profiles IPR023332 Proteasome A-type subunit 19 229 7
PTHR11599:SF15 HMMPANTHER 2 248 1
PTHR11599 HMMPANTHER 2 248 14 Gene3D 3 238 19
SSF56235 SuperFamily Nucleophile aminohydrolases, N-terminal 2 235 22

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.18 Da
Charge 2.00
Isoelectric point 7.57
Molecular weight 28.28 kDa
Number of residues 259

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
present during cellular response to thiabendazole
MOD:00696phosphorylated residueNASPMID:182575171922
Gene Expression

Qualitative Gene Expression

protein levelunchanged during GO:0044838Western blot evidencePMID:20133687
present during GO:0072690Western blot evidencePMID:20133687

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
51617during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
53609during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
47724during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
49679during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
20169.47during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
51105during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
25962during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8.8during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3425
conserved in vertebrates3400
conserved in archaea237

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
rpn219S proteasome regulatory subunit Rpn2 (predicted) Affinity Capture-MSPMID:20133687
scl120S proteasome complex subunit alpha 1 (predicted) Affinity Capture-MSPMID:20133687
bag102BAG family molecular chaperone regulator Bag102 Affinity Capture-WesternPMID:24497846
Reconstituted Complex
mts419S proteasome regulatory subunit Mts4 Affinity Capture-MSPMID:20133687
pre920S proteasome complex subunit alpha 3 Pre9 (predicted) Affinity Capture-MSPMID:20133687
ecm29proteasome assembly chaperone Ecm29 Affinity Capture-MSPMID:20133687
pre520S proteasome complex subunit alpha 6 subunit Pre5 (predicted) Affinity Capture-MSPMID:20133687
rpn719S proteasome regulatory subunit Rpn7 Affinity Capture-MSPMID:20133687
rpt119S proteasome regulatory subunit Rpt1 (predicted) Affinity Capture-MSPMID:22307589
Affinity Capture-MSPMID:20133687
uch2ubiquitin C-terminal hydrolase Uch2 Affinity Capture-MSPMID:20838651
Affinity Capture-MSPMID:20133687
rpt319S proteasome regulatory subunit Rpt3 (predicted) Affinity Capture-MSPMID:20133687
rpn619S proteasome regulatory subunit Rpn6 (predicted) Affinity Capture-MSPMID:20133687
bag101BAG family molecular chaperone regulator Bag101 (predicted) Reconstituted ComplexPMID:24497846
Affinity Capture-Western
pre820S proteasome complex subunit alpha 2, Pre8 (predicted) Affinity Capture-MSPMID:20133687
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
rpn1119S proteasome regulatory subunit Rpn11 Affinity Capture-MSPMID:20838651
Affinity Capture-MSPMID:20133687
rpn319S proteasome regulatory subunit Rpn3 Affinity Capture-MSPMID:20133687
rpn50219S proteasome regulatory subunit Rpn502 Affinity Capture-MSPMID:20133687
pam120S proteasome complex subunit beta 6 Affinity Capture-MSPMID:20133687
rpn919S proteasome regulatory subunit Rpn9 Affinity Capture-MSPMID:20133687
rpn130119S proteasome regulatory subunit Rpn13a Affinity Capture-MSPMID:20133687
pre120S proteasome complex subunit beta 4 Pre1 (predicted) Affinity Capture-MSPMID:20133687
pup120S proteasome complex subunit beta 2 (predicted) Affinity Capture-MSPMID:20133687
pup220S proteasome complex subunit alpha 5, Pup2 (predicted) Affinity Capture-MSPMID:20133687
rpn1019S proteasome regulatory subunit Rpn10 Affinity Capture-MSPMID:20133687
pre620S proteasome complex subunit alpha 4 Pre6 Affinity Capture-MSPMID:20133687
rpt419S proteasome regulatory subunit Rpt4 (predicted) Affinity Capture-MSPMID:20133687
pre320S proteasome complex subunit beta 1 Pre3 (predicted) Affinity Capture-MSPMID:20133687
rpn819S proteasome regulatory subunit Rpn8 (predicted) Affinity Capture-MSPMID:20133687
ump1proteasome maturation factor Ump1 (predicted) Affinity Capture-MSPMID:20133687
rpn130219S proteasome regulatory subunit Rpn13b Affinity Capture-MSPMID:20133687
rpt219S proteasome regulatory subunit Rpt2 Affinity Capture-MSPMID:20133687
pts120S proteasome complex subunit beta 5 Affinity Capture-MSPMID:20133687
pre420S proteasome complex subunit beta 7, Pre4 (predicted) Affinity Capture-MSPMID:20133687
pre1020S proteasome complex subunit alpha 7, Pre10 (predicted) Affinity Capture-MSPMID:20133687
pup320S proteasome complex subunit beta 3, Pup3 (predicted) Affinity Capture-MSPMID:20133687
rpn1219S proteasome regulatory subunit Rpn12 Affinity Capture-MSPMID:20133687
rpn50119S proteasome regulatory subunit Rpn501 Affinity Capture-MSPMID:20133687
rpt619S proteasome regulatory subunit Rpt6 (predicted) Affinity Capture-MSPMID:20133687
rpt519S proteasome regulatory subunit Rpt5 (predicted) Affinity Capture-MSPMID:20133687
External References
Database Identifier Description
NBRP SPBC106.16 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC106.16 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC106.16 BioGRID Interaction Datasets
Expression Viewer SPBC106.16 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC106.16 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC106.16 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC106.16 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC106.16 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC106.16 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC106.16 Cell Cycle Data
GEO SPBC106.16 GEO profiles
PInt SPBC106.16 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC106.16 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC106.16 Fission yeast phenotypic data & analysis
IntEnz3.4.25.1Integrated relational Enzyme database
Rhea3.4.25.1Annotated reactions database
SPD / RIKEN13/13E11Orfeome Localization Data
UniProtKB/SwissProtQ10329Probable proteasome subunit alpha type-4
ModBaseQ10329Database of comparative protein structure models
STRINGQ10329Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_59516520S proteasome component alpha 4 Pre6
RefSeq mRNANM_001021074972h- 20S proteasome component alpha 4 Pre6 (pre6), mRNA
European Nucleotide ArchiveCAB53732.1ENA Protein Mapping
UniParcUPI000013246BUniProt Archive

Literature for pre6

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014