pre6 (SPBC106.16)

Gene Standard Namepre6 Characterisation Statuspublished
Systematic IDSPBC106.16 Feature Typeprotein coding
Synonyms Name Description
Product20S proteasome complex subunit alpha 4 Pre6 Product Size259aa, 28.28 kDa
Genomic Location Chromosome II, 409158-410766 (1609nt); CDS:409496-410453 (958nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
endopeptidase activity66
Annotation ExtensionEvidenceWith/FromReference
threonine-type endopeptidase activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
loss of viability at high temperaturemts7-172
normal growth on cisplatinpre6Δ39
resistance to brefeldin Amts7-127
resistance to caffeinemts7-135
resistance to carbendazimmts7-114
resistance to staurosporinemts7-116
viable vegetative cell populationpre6Δ3862

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologypre6Δ3103
Target Of
FYPO affected by mutation in cut8 tethering factor for nuclear proteasome Cut8
GO transported by crm1 importin family nuclear export receptor Crm1
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons409158..409594, 409655..410028, 410147..410766
Intron409595..409654, 410029..410146
5' UTR409158..409495PMID:21511999
CDS409496..409594, 409655..410028, 410147..410453
3' UTR410454..410766PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF10584 Pfam IPR000426 Proteasome alpha-subunit, N-terminal domain 4 26 7
PF00227 Pfam IPR001353 Proteasome, subunit alpha/beta 27 210 14
SM00948 SMART IPR000426 Proteasome alpha-subunit, N-terminal domain 4 26 7
PS00388 Prosite Patterns IPR000426 Proteasome alpha-subunit, N-terminal domain 4 26 6
PS51475 Prosite Profiles IPR023332 Proteasome A-type subunit 19 229 7
PTHR11599:SF15 HMMPANTHER 2 240 1
PTHR11599 HMMPANTHER 2 240 14 Gene3D IPR029055 Nucleophile aminohydrolases, N-terminal 3 238 19
SSF56235 SuperFamily IPR029055 Nucleophile aminohydrolases, N-terminal 2 235 22

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.18 Da
Charge 2.00
Codon Adaptation Index 0.49
Isoelectric point 7.57
Molecular weight 28.28 kDa
Number of residues 259

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2293
present during cellular response to thiabendazoleS12
S9, S12
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S12 PMID:18257517
IDA S9 PMID:25720772
IDA S12 PMID:25720772
phosphorylated residue 2515
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Gene Expression

Qualitative Gene Expression

protein levelpresent during GO:0072690Western blot evidencePMID:20133687
unchanged during GO:0044838Western blot evidencePMID:20133687

Quantitative Gene Expression

View graphical display of gene expression data for pre6 (SPBC106.16)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
51617during GO:0000080PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
53609during GO:0000084PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
47724during GO:0000085PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
49679during GO:0000087PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
51105during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
20169.47during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
25962during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8.8during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
predominantly single copy (one to one)3087
conserved in fungi4608
conserved in eukaryotes4516
conserved in bacteria1005
conserved in metazoa3498
conserved in vertebrates3473
conserved in archaea243

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC106.16 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesrpt119S proteasome regulatory subunit Rpt1 (predicted) Affinity Capture-MSPMID:22307589
affinity capturesrpn50119S proteasome regulatory subunit Rpn501 Affinity Capture-MSPMID:20133687
affinity capturespre620S proteasome complex subunit alpha 4 Pre6 Affinity Capture-MSPMID:20133687
affinity capturesrpn130219S proteasome regulatory subunit Rpn13b Affinity Capture-MSPMID:20133687
affinity capturesecm29proteasome assembly chaperone Ecm29 Affinity Capture-MSPMID:20133687
affinity capturesrpn619S proteasome regulatory subunit Rpn6 (predicted) Affinity Capture-MSPMID:20133687
affinity capturesump1proteasome maturation factor Ump1 (predicted) Affinity Capture-MSPMID:20133687
affinity capturespts120S proteasome complex subunit beta 5 Affinity Capture-MSPMID:20133687
affinity capturespre320S proteasome complex subunit beta 1 Pre3 (predicted) Affinity Capture-MSPMID:20133687
affinity capturespup120S proteasome complex subunit beta 2 (predicted) Affinity Capture-MSPMID:20133687
affinity capturesrpn130119S proteasome regulatory subunit Rpn13a Affinity Capture-MSPMID:20133687
affinity capturesmts419S proteasome regulatory subunit Mts4 Affinity Capture-MSPMID:20133687
affinity capturesrpt219S proteasome regulatory subunit Rpt2 Affinity Capture-MSPMID:20133687
affinity capturesrpt419S proteasome regulatory subunit Rpt4 (predicted) Affinity Capture-MSPMID:20133687
affinity capturesuch2ubiquitin C-terminal hydrolase Uch2 Affinity Capture-MSPMID:20133687
affinity capturespre920S proteasome complex subunit alpha 3 Pre9 (predicted) Affinity Capture-MSPMID:20133687
affinity capturespup320S proteasome complex subunit beta 3, Pup3 (predicted) Affinity Capture-MSPMID:20133687
affinity capturesrpn1119S proteasome regulatory subunit Rpn11 Affinity Capture-MSPMID:20133687
affinity capturesrpt319S proteasome regulatory subunit Rpt3 (predicted) Affinity Capture-MSPMID:20133687
affinity capturesrpn319S proteasome regulatory subunit Rpn3 Affinity Capture-MSPMID:20133687
affinity capturespre120S proteasome complex subunit beta 4 Pre1 (predicted) Affinity Capture-MSPMID:20133687
affinity capturesrpn1019S proteasome regulatory subunit Rpn10 Affinity Capture-MSPMID:20133687
affinity capturesrpt519S proteasome regulatory subunit Rpt5 (predicted) Affinity Capture-MSPMID:20133687
affinity capturespre820S proteasome complex subunit alpha 2, Pre8 (predicted) Affinity Capture-MSPMID:20133687
affinity capturesscl120S proteasome complex subunit alpha 1 (predicted) Affinity Capture-MSPMID:20133687
affinity capturespre420S proteasome complex subunit beta 7, Pre4 (predicted) Affinity Capture-MSPMID:20133687
affinity capturesrpn219S proteasome regulatory subunit Rpn2 (predicted) Affinity Capture-MSPMID:20133687
affinity capturesrpn1219S proteasome regulatory subunit Rpn12 Affinity Capture-MSPMID:20133687
affinity capturespam120S proteasome complex subunit beta 6 Affinity Capture-MSPMID:20133687
affinity capturespre520S proteasome complex subunit alpha 6 subunit Pre5 (predicted) Affinity Capture-MSPMID:20133687
affinity capturesrpn719S proteasome regulatory subunit Rpn7 Affinity Capture-MSPMID:20133687
affinity capturespre1020S proteasome complex subunit alpha 7, Pre10 (predicted) Affinity Capture-MSPMID:20133687
affinity capturespup220S proteasome complex subunit alpha 5, Pup2 (predicted) Affinity Capture-MSPMID:20133687
affinity capturesrpt619S proteasome regulatory subunit Rpt6 (predicted) Affinity Capture-MSPMID:20133687
affinity capturesrpn50219S proteasome regulatory subunit Rpn502 Affinity Capture-MSPMID:20133687
affinity capturesrpn919S proteasome regulatory subunit Rpn9 Affinity Capture-MSPMID:20133687
affinity capturesrpn819S proteasome regulatory subunit Rpn8 (predicted) Affinity Capture-MSPMID:20133687
affinity captured byrpn1119S proteasome regulatory subunit Rpn11 Affinity Capture-MSPMID:20838651
affinity captured byuch2ubiquitin C-terminal hydrolase Uch2 Affinity Capture-MSPMID:20838651
modified bylkh1dual specificity protein kinase Lkh1 Biochemical ActivityPMID:26167880
modified bydsk1SR protein-specific kinase Dsk1 Biochemical ActivityPMID:26167880
External References
Database Identifier Description
NBRP SPBC106.16 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC106.16 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC106.16 BioGRID Interaction Datasets
Expression Viewer SPBC106.16 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC106.16 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC106.16 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC106.16 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC106.16 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC106.16 Transcriptome Viewer (Bähler Lab)
GEO SPBC106.16 GEO profiles
PInt SPBC106.16 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC106.16 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC106.16 Fission yeast phenotypic data & analysis
Cyclebase SPBC106.16.1 Cell Cycle Data
IntEnz3.4.25.1Integrated relational Enzyme database
Rhea3.4.25.1Annotated reactions database
SPD / RIKEN13/13E11Orfeome Localization Data
UniProtKB/SwissProtQ10329Probable proteasome subunit alpha type-4
ModBaseQ10329Database of comparative protein structure models
STRINGQ10329Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_59516520S proteasome component alpha 4 Pre6
RefSeq mRNANM_001021074972h- 20S proteasome component alpha 4 Pre6 (pre6), mRNA
European Nucleotide ArchiveCAB53732.1ENA Protein Mapping
UniParcUPI000013246BUniProt Archive

Literature for pre6

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017