cys2 (SPBC106.17c)


Gene Standard Namecys2 Characterisation Statuspublished
Systematic IDSPBC106.17c Feature Typeprotein coding
Synonyms Name Description
Producthomoserine O-acetyltransferase (predicted) Product Size504aa, 56.36 kDa
Genomic Location Chromosome II, 412795-410720 (2076nt); CDS:412634-411120 (1515nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
homoserine O-acetyltransferase activity2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cysteine biosynthetic process5
Annotation ExtensionEvidenceWith/FromReference
methionine biosynthetic process20
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4212
Annotation ExtensionEvidenceWith/FromReference
mitochondrion752
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
growth auxotrophic for cysteinecys2-1 (G426D)9
normal growth on sodium butyratecys2Δ55
normal growth on trichostatin Acys2Δ114
sensitive to hydroxyureacys2Δ580
sensitive to valproic acidcys2Δ155
viable vegetative cell populationcys2Δ3844

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologycys2Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons412795..410720
mRNA412795..410720
5' UTR412795..412635PMID:21511999
CDS412634..411120
3' UTR411119..410720PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00561 Pfam IPR000073 Alpha/beta hydrolase fold-1 117 395 6
PTHR10992 HMMPANTHER 431 503 4
PTHR10992 HMMPANTHER 32 323 4
PTHR10992:SF756 HMMPANTHER 431 503 1
PTHR10992:SF756 HMMPANTHER 32 323 1
3.40.50.1820 Gene3D IPR029058 Alpha/Beta hydrolase fold 434 481 41
3.40.50.1820 Gene3D IPR029058 Alpha/Beta hydrolase fold 371 372 41
3.40.50.1820 Gene3D IPR029058 Alpha/Beta hydrolase fold 100 287 41
SSF53474 SuperFamily IPR029058 Alpha/Beta hydrolase fold 422 501 42
SSF53474 SuperFamily IPR029058 Alpha/Beta hydrolase fold 83 363 42
PIRSF000443 PIRSF IPR008220 Homoserine acetyltransferase 33 504 2
TIGR01392 TIGRFAM IPR008220 Homoserine acetyltransferase 87 371 2

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000646abhydrolase familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000646

Protein Properties

Ave. residue weight 111.83 Da
Charge 4.00
Codon Adaptation Index 0.48
Isoelectric point 6.89
Molecular weight 56.36 kDa
Number of residues 504
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for cys2 (SPBC106.17c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4607during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4421during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4517during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4425during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4778during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
40045.98during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3486.84during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Experimental Tools
DescriptionQualifierReferenceCount
selectable marker, amino acid auxotroph PMID:1762253310
Taxonomic Conservation
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog601
conserved in fungi4606
conserved in eukaryotes4516
conserved in bacteria1002
Orthologs

Orthologs in Compara


External References
Database Identifier Description
NBRP SPBC106.17c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC106.17c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC106.17c BioGRID Interaction Datasets
Expression Viewer SPBC106.17c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC106.17c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC106.17c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC106.17c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC106.17c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC106.17c Transcriptome Viewer (Bähler Lab)
GEO SPBC106.17c GEO profiles
PInt SPBC106.17c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC106.17c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC106.17c Fission yeast phenotypic data & analysis
Cyclebase SPBC106.17c.1 Cell Cycle Data
IntEnz2.3.1.-Integrated relational Enzyme database
Rhea2.3.1.-Annotated reactions database
SPD / RIKEN44/44H01Orfeome Localization Data
UniProtKB/SwissProtQ10341Probable serine-O-acetyltransferase cys2
ModBaseQ10341Database of comparative protein structure models
STRINGQ10341Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595166homoserine O-acetyltransferase (predicted)
RefSeq mRNANM_001021075972h- homoserine O-acetyltransferase (predicted) (cys2), mRNA
European Nucleotide ArchiveCAB53733.1ENA Protein Mapping
MetaCycPWY-5344L-homocysteine biosynthesis
KEGG00270+2.3.1.31Cysteine and methionine metabolism
UniParcUPI000013A3ACUniProt Archive
UniPathwayUPA00051Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3

Literature for cys2

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016