cys2 (SPBC106.17c)


Gene Standard Namecys2 Characterisation Statuspublished
Systematic IDSPBC106.17c Feature Typeprotein coding
Synonyms Name Description
Producthomoserine O-acetyltransferase (predicted) Product Size504aa, 56.36 kDa
Genomic Location Chromosome II, 412795-410720 (2076nt); CDS:412634-411120 (1515nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
homoserine O-acetyltransferase activity2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cysteine biosynthetic process11
Annotation ExtensionEvidenceWith/FromReference
methionine biosynthetic process30
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4205
Annotation ExtensionEvidenceWith/FromReference
mitochondrion758
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000037growth auxotrophic for cysteineCell growth assaycys2-1 (G426D)Not specifiedPECO:0000126, PECO:0000005, PECO:0000102PMID:176225339
FYPO:0002619normal growth on sodium butyrateCell growth assaycys2ΔNullPECO:0000005, PECO:0000201, PECO:0000142PMID:2386193755
FYPO:0002620normal growth on trichostatin ACell growth assaycys2ΔNullPECO:0000005, PECO:0000201, PECO:0000142PMID:23861937110
FYPO:0000088sensitive to hydroxyurea514
expressivity FYPO_EXT:0000003Cell Growth Assaycys2ΔNullPECO:0000137, PECO:0000102PMID:23697806
FYPO:0000115sensitive to valproic acid155
expressivity FYPO_EXT:0000003Cell growth assaycys2ΔNullPECO:0000201, PECO:0000005, PECO:0000142PMID:23861937
FYPO:0002060viable vegetative cell populationMicroscopycys2ΔNullPMID:126183703759
Microscopycys2ΔNullPMID:20473289
Microscopycys2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopycys2ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1412795410720

UTRs

Region Coordinates Reference
five_prime_UTR412795..412635PMID:21511999
three_prime_UTR411119..410720PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00561 Pfam IPR000073 Alpha/beta hydrolase fold-1 158 395 5
PTHR10992 HMMPANTHER 42 502 5
PTHR10992:SF262 HMMPANTHER 42 502 1
3.40.50.1820 Gene3D 100 287 41
3.40.50.1820 Gene3D 434 481 41
3.40.50.1820 Gene3D 371 372 41
SSF53474 SuperFamily Alpha/Beta hydrolase fold 422 501 42
SSF53474 SuperFamily Alpha/Beta hydrolase fold 83 363 42
PIRSF000443 PIRSF IPR008220 Homoserine acetyltransferase 22 504 2
TIGR01392 tigrfam IPR008220 Homoserine acetyltransferase 87 371 2

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000646abhydrolase familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000646

Protein Properties

Ave. residue weight 111.83 Da
Charge 4.00
Isoelectric point 6.89
Molecular weight 56.36 kDa
Number of residues 504
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4607during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
4421during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
4517during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
4425during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
40045.98during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
4778during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3486.84during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Experimental Tools
DescriptionQualifierReferenceCount
selectable marker, amino acid auxotroph PMID:1762253310
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog602
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
Orthologs

Orthologs in Compara


External References
Database Identifier Description
NBRP SPBC106.17c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC106.17c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC106.17c BioGRID Interaction Datasets
Expression Viewer SPBC106.17c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC106.17c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC106.17c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC106.17c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC106.17c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC106.17c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC106.17c Cell Cycle Data
GEO SPBC106.17c GEO profiles
PInt SPBC106.17c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC106.17c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC106.17c Fission yeast phenotypic data & analysis
SPD / RIKEN44/44H01Orfeome Localization Data
IntEnz2.3.1.-Integrated relational Enzyme database
Rhea2.3.1.-Annotated reactions database
UniProtKB/SwissProtQ10341Probable serine-O-acetyltransferase cys2
ModBaseQ10341Database of comparative protein structure models
STRINGQ10341Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595166homoserine O-acetyltransferase (predicted)
RefSeq mRNANM_001021075972h- homoserine O-acetyltransferase (predicted) (cys2), mRNA
European Nucleotide ArchiveCAB53733.1ENA Protein Mapping
UniParcUPI000013A3ACUniProt Archive

Literature for cys2

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014