exo70 (SPBC106.20)

Gene Standard Nameexo70 ChromosomeII
Systematic IDSPBC106.20 Gene Start417141
SynonymsSPBC582.02 Gene End419867
Productexocyst complex subunit Exo70 (predicted) Gene Length2727
Feature Typeprotein coding CDS Start417319
Name Description CDS End419476
Characterisation Statuspublished CDS Length2158

Ensembl Gene Location
  1. cys2
  2. SPNCRNA.1355
  3. rpl2501
  4. SPBC106.19
  5. exo70
  6. cdc13
  7. dsh1
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0001587normal protein localization to cell tipProtein localization experimentexo70delta (deletion)deletionPECO:0000004PMID:22768263
assayed_using SPCC622.10c
assayed_using SPAC6F12.08c
assayed_using sec3
FYPO:0002060viable vegetative cell populationexo70delta (deletion)deletionPMID:12618370
Microscopyexo70delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0005515protein bindingIPIsec8PMID:11854409
IPIsec10PMID:11854409
IPIsec6PMID:11854409
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0000920cytokinetic cell separationTASPMID:11854409
GO:0006886intracellular protein transportICGO:0006887GO_REF:0000001
GO:0006887exocytosisICGO:0000145GO_REF:0000001
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0000145exocystIDAPMID:11854409
GO:0005634nucleusIDAPMID:16823372
GO:0005829cytosolIDAPMID:16823372
GO:0032153cell division site
exists_during mitotic prophaseIDAPMID:11854409
GO:0051285cell cortex of cell tipIDAPMID:11854409
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1417141417320
2417371417399
3417460417618
4417665419219
5419273419309
6419411419867

References

Region Start End Reference
three_prime_UTR419477419867PMID:21511999
five_prime_UTR417141417318PMID:21511999
three_prime_UTR419477419867PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF03081 IPR004140 Pfam Exocyst complex protein Exo70 250 608 1
SSF74788 IPR016159 SuperFamily 104 611 6
PTHR12542 IPR004140 hmmpanther Exocyst complex protein Exo70 6 611 1
PTHR12542:SF7 hmmpanther 6 611 1

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residuePMID:18257517

Protein Properties

Ave. residue weight 112.26 Da
Charge -6.00
Isoelectric point 5.44
Molecular weight 69.04 kDa
Number of residues 615
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide775.37PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide414.57PMID:23101633
RNA levelexperimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide1.4PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide0.5PMID:23101633
Species Distribution
DescriptionQualifierReference
predominantly single copy (one to one)
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
elp2elongator complex subunit Elp2 (predicted) Negative GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
hus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
myo52myosin type V Phenotypic EnhancementPMID:21148300
Synthetic Lethality
Synthetic Growth DefectPMID:21652630
pob1Boi family protein Synthetic Growth DefectPMID:21401840
sec3exocyst complex subunit Sec3 Synthetic LethalityPMID:22891673
Synthetic LethalityPMID:22768263
sec8exocyst complex subunit Sec8 Synthetic LethalityPMID:21652630
dcc1Ctf18 RFC-like complex subunit Dcc1 Positive GeneticPMID:22681890
par1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
aut12autophagy associated protein Aut12 (predicted) Positive GeneticPMID:22681890
SPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:22681890
gyp10GTPase activating protein Gyp10 Synthetic Growth DefectPMID:16291723
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
rho3Rho family GTPase Rho3 Synthetic Growth DefectPMID:12930742
Dosage Rescue
int6eIF3e subunit Int6 Positive GeneticPMID:22681890
for3formin For3 Synthetic LethalityPMID:21148300
Phenotypic Enhancement
Synthetic Growth DefectPMID:21652630
Phenotypic Enhancement
vps35retromer complex subunit Vps35 Positive GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cdc42Rho family GTPase Cdc42 Two-hybridPMID:21899677
Affinity Capture-Western
pob1Boi family protein Two-hybridPMID:21899677
sec8exocyst complex subunit Sec8 Affinity Capture-WesternPMID:11854409
chs2chitin synthase homolog Chs2 Co-localizationPMID:16772338
eng1endo-1,3-beta-glucanase Eng1 Co-localizationPMID:16079182
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External References
Database Identifier Description
NBRP SPBC106.20 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC106.20 Retrieval of eukaryotic orthologs
BioGrid SPBC106.20 BioGRID Interaction Datasets
Bähler Lab SPBC106.20 Cell Cycle Expression Profile
Bähler Lab SPBC106.20 Meiosis/Sporulation Expression Profies
Bähler Lab SPBC106.20 Pheromone response/mating expression profiles
Bähler Lab SPBC106.20 Environmental stress expression profiles
Bähler Lab SPBC106.20 Bähler Lab Transcriptome Viewer
Cyclebase SPBC106.20 Cell Cycle Data
PInt SPBC106.20 Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPBC106.20 Entrez Gene
EntrezGene2540127exocyst complex subunit Exo70 (predicted)
Uniprot_genenameEXO70
WikiGene2540127exocyst complex subunit Exo70 (predicted)
SPD / RIKEN37/37F08Orfeome Localization Data
UniProtKB/SwissProtQ10339Exocyst complex component exo70
ModBaseQ10339Database of comparative protein structure models
Pfam Protein DomainsQ10339Pfam Domain Arrangement
RefSeq PeptideNP_595170exocyst complex subunit Exo70 (predicted)
RefSeq mRNANM_001021078972h- exocyst complex subunit Exo70 (predicted) (exo70), mRNA
European Nucleotide ArchiveCAB53736ENA Protein Mapping
UniParcUPI000012A2D9UniProt Archive

Literature for exo70
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013