rav1 (SPBC1105.10)


Gene Standard Namerav1 Characterisation Statuspublished
Systematic IDSPBC1105.10 Feature Typeprotein coding
Synonyms Name Description
ProductRAVE complex subunit Rav1 Product Size1297aa, 148.74 kDa
Genomic Location Chromosome II, 3524531-3528845 (4315nt); CDS:3524673-3528681 (4009nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein complex binding47
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular response to calcium ion5
Annotation ExtensionEvidenceWith/FromReference
cellular response to zinc ion1
Annotation ExtensionEvidenceWith/FromReference
late endosome to vacuole transport44
Annotation ExtensionEvidenceWith/FromReference
vacuolar acidification17
Annotation ExtensionEvidenceWith/FromReference
vacuolar proton-transporting V-type ATPase complex assembly4
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4206
Annotation ExtensionEvidenceWith/FromReference
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
endosome membrane22
Annotation ExtensionEvidenceWith/FromReference
nucleus2697
Annotation ExtensionEvidenceWith/FromReference
RAVE complex3
Annotation ExtensionEvidenceWith/FromReference
vacuole185
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased mating efficiencyrav1ΔNull247
sensitive to cadmiumrav1ΔNull246
sensitive to camptothecinrav1ΔNull219
sensitive to doxorubicinrav1ΔNull91
rav1ΔNull
sensitive to epirubicinrav1ΔNull2
sensitive to hydrogen peroxiderav1ΔNull137
sensitive to hydroxyurearav1ΔNull525
sensitive to salt stressrav1ΔNull86
viable vegetative cell populationrav1ΔNull3783

Cell Phenotype

Term NameAlleleExpressionCount
increased cellular doxorubicin levelrav1ΔNull2
viable vegetative cell with normal cell morphologyrav1ΔNull3092
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
135245313524759
235248753528845

UTRs

Region Coordinates Reference
five_prime_UTR3524531..3524672PMID:21511999
three_prime_UTR3528682..3528845PMID:21511999
mRNA3524531..3528845
exon3524673..3524759,3524875..3528681
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF12234 Pfam IPR022033 RAVE complex protein Rav1 C-terminal 574 1231 1
PTHR13950 HMMPANTHER 502 1296 1
2.130.10.10 Gene3D IPR015943 WD40/YVTN repeat-like-containing domain 540 670 145
2.130.10.10 Gene3D IPR015943 WD40/YVTN repeat-like-containing domain 96 238 145
0049172 SuperFamily 24 270 4
SSF50978 SuperFamily IPR017986 WD40-repeat-containing domain 382 726 135

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000190WD repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000190

Protein Properties

Ave. residue weight 114.68 Da
Charge 6.50
Isoelectric point 6.82
Molecular weight 148.74 kDa
Number of residues 1297
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1043during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1305during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
239.65during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1056during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
526.23during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.3during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.44during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesskp1SCF ubiquitin ligase complex subunit Skp1 Affinity Capture-WesternPMID:18441123
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction witharp8actin-like protein, Ino80 complex subunit Arp8 Positive GeneticPMID:22681890
positive genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
synthetically rescued bycor1cornichon family protein (predicted) Synthetic RescuePMID:24059229
synthetic growth defect withcor1cornichon family protein (predicted) Synthetic Growth DefectPMID:24059229
negative genetic interaction withcut2securin, sister chromatid separation inhibitor Negative GeneticPMID:22681890
phenotype enhanced bydad5DASH complex subunit Dad5 Phenotypic EnhancementPMID:23365689
positive genetic interaction witherg31C-5 sterol desaturase Erg31 Positive GeneticPMID:22681890
negative genetic interaction withhta1histone H2A alpha Negative GeneticPMID:22681890
phenotype enhanced byiec1Ino80 complex subunit Iec1 Phenotypic EnhancementPMID:23365689
positive genetic interaction withiwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
phenotype enhanced bylac1sphingosine N-acyltransferase Lac1 Phenotypic EnhancementPMID:18441123
phenotype enhanced bylag1sphingosine N-acyltransferase Lag1 Phenotypic EnhancementPMID:18441123
negative genetic interaction withmcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
negative genetic interaction withmpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
negative genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
phenotype enhanced bypmd1leptomycin transmembrane transporter Pmd1 Phenotypic EnhancementPMID:24059229
synthetic growth defect withpmd1leptomycin transmembrane transporter Pmd1 Synthetic Growth DefectPMID:24059229
negative genetic interaction withprp43ATP-dependent RNA helicase Prp43 Negative GeneticPMID:22681890
negative genetic interaction withrad51RecA family recombinase Rad51/Rhp51 Negative GeneticPMID:22681890
phenotype enhanced byrad55RecA family ATPase Rad55/Rhp55 Phenotypic EnhancementPMID:23365689
synthetic growth defect withrad55RecA family ATPase Rad55/Rhp55 Synthetic Growth DefectPMID:23365689
positive genetic interaction withsdc1Dpy-30 domain protein Sdc1 Positive GeneticPMID:22681890
negative genetic interaction withsfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsif1Sad1 interacting factor 1 Negative GeneticPMID:22681890
negative genetic interaction withsnf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
positive genetic interaction withste20Rictor homolog, Ste20 Positive GeneticPMID:22681890
positive genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withubp2ubiquitin C-terminal hydrolase Ubp2 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC1105.10 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1105.10 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1105.10 BioGRID Interaction Datasets
Expression Viewer SPBC1105.10 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1105.10 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1105.10 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1105.10 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1105.10 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1105.10 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1105.10 Cell Cycle Data
GEO SPBC1105.10 GEO profiles
PInt SPBC1105.10 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1105.10 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1105.10 Fission yeast phenotypic data & analysis
SPD / RIKEN48/48F07Orfeome Localization Data
UniProtKB/SwissProtQ9Y817Regulator of V-ATPase in vacuolar membrane protein 1
ModBaseQ9Y817Database of comparative protein structure models
STRINGQ9Y817Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596466RAVE complex subunit Rav1
RefSeq mRNANM_001022385972h- RAVE complex subunit Rav1 (rav1), mRNA
European Nucleotide ArchiveCAB50973.1ENA Protein Mapping
UniParcUPI00000696DFUniProt Archive

Literature for rav1

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015