tfg2 (SPBC1198.13c)


Gene Standard Nametfg2 Characterisation Statusbiological_role_inferred
Systematic IDSPBC1198.13c Feature Typeprotein coding
SynonymsSPBC660.03c Name Description
Producttranscription factor TFIIF complex beta subunit Tfg2 (predicted) Product Size307aa, 34.62 kDa
Genomic Location Chromosome II, 197189-194038 (3152nt); CDS:197075-195990 (1086nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
ATP-dependent DNA helicase activity41
Annotation ExtensionEvidenceWith/FromReference
core RNA polymerase II binding transcription factor activity33
Annotation ExtensionEvidenceWith/FromReference
DNA binding379
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
mitotic spindle pole body216
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
transcription factor TFIIF complex3
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationtfg2ΔNull1438

Cell Phenotype

Term NameAlleleExpressionCount
inviable after spore germination, without cell division, with abnormal germ tube morphologytfg2ΔNull153
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons197189..196551, 196491..196348, 196244..194038
mRNA197189..194038
5' UTR197189..197076PMID:21511999
CDS197075..196551, 196491..196348, 196244..195990
3' UTR195989..194038PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02270 Pfam IPR003196 Transcription initiation factor IIF, beta subunit 21 253 1
PTHR10445:SF0 HMMPANTHER 2 307 1
PTHR10445 HMMPANTHER IPR003196 Transcription initiation factor IIF, beta subunit 2 307 1
1.10.10.10 Gene3D IPR011991 Winged helix-turn-helix DNA-binding domain 190 253 46
SSF50916 SuperFamily IPR011039 Transcription Factor IIF, Rap30/Rap74, interaction 17 124 2
SSF46785 SuperFamily 189 254 49

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.78 Da
Charge -7.00
Isoelectric point 4.88
Molecular weight 34.62 kDa
Number of residues 307
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
17849during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
17763during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
18474during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
19608during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3533.66during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
18306during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
880.94during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byfcp1CTD phosphatase Fcp1 Affinity Capture-MSPMID:11839823
affinity captured byfcp1CTD phosphatase Fcp1 Affinity Capture-WesternPMID:15616156
affinity captured bymtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
co-purifies withrpb3RNA polymerase II subunit 3 Co-purificationPMID:15620689
affinity captured byrpb3RNA polymerase II subunit 3 Affinity Capture-MSPMID:11839823
affinity captured byrpb5DNA-directed RNA polymerase I, II and III subunit Rpb5 Affinity Capture-WesternPMID:15743411
affinity captured byrpc10DNA-directed RNA polymerase I, II and III subunit Rpc10 Affinity Capture-WesternPMID:15743411
affinity captured bytfg3transcription factor TFIIF complex subunit Tfg3 Affinity Capture-WesternPMID:15616156
External References
Database Identifier Description
NBRP SPBC1198.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1198.13c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1198.13c BioGRID Interaction Datasets
Expression Viewer SPBC1198.13c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1198.13c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1198.13c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1198.13c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1198.13c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1198.13c Transcriptome Viewer (Bähler Lab)
GEO SPBC1198.13c GEO profiles
PInt SPBC1198.13c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1198.13c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1198.13c Fission yeast phenotypic data & analysis
Cyclebase SPBC1198.13c.1 Cell Cycle Data
SPD / RIKEN16/16H07Orfeome Localization Data
UniProtKB/SwissProtO94424Transcription initiation factor IIF subunit beta
ModBaseO94424Database of comparative protein structure models
STRINGO94424Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595082transcription factor TFIIF complex beta subunit Tfg2 (predicted)
RefSeq mRNANM_001020988972h- transcription factor TFIIF complex beta subunit Tfg2 (predicted) (tfg2), mRNA
European Nucleotide ArchiveCAB91188.1ENA Protein Mapping
UniParcUPI000006BB4AUniProt Archive

Literature for tfg2

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015