tfg2 (SPBC1198.13c)


Gene Standard Nametfg2 Characterisation Statusbiological_role_inferred
Systematic IDSPBC1198.13c Feature Typeprotein coding
SynonymsSPBC660.03c Name Description
Producttranscription factor TFIIF complex beta subunit Tfg2 (predicted) Product Size307aa, 34.62 kDa
Genomic Location Chromosome II, 197189-194038 (3152nt); CDS:197075-195990 (1086nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0004003ATP-dependent DNA helicase activityISMPFAM:PF02270GO_REF:000002442
GO:0003677DNA bindingIEAUniProtKB-KW:KW-0238GO_REF:0000037383
GO:0000991core RNA polymerase II binding transcription factor activityISSUniProtKB:P41896GO_REF:000002433
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032508DNA duplex unwindingISMPFAM:PF02270GO_REF:000000131
GO:0006357regulation of transcription from RNA polymerase II promoterISSUniProtKB:P41896GO_REF:0000001291
GO:0006367transcription initiation from RNA polymerase II promoterISSUniProtKB:P41896GO_REF:000002436
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0044732mitotic spindle pole bodyIDAPMID:16823372216
GO:0005634nucleusIDAPMID:168233722740
GO:0005674transcription factor TFIIF complexISSUniProtKB:P41896GO_REF:00000243
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopytfg2ΔNullPMID:204732891338
Microscopytfg2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000313inviable after spore germination, without cell division, with abnormal germ tube morphology146
penetrance FYPO_EXT:0000001Microscopytfg2ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1197189196551
2196491196348
3196244194038

UTRs

Region Coordinates Reference
five_prime_UTR197189..197076PMID:21511999
three_prime_UTR195989..194038PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02270 Pfam IPR003196 Transcription initiation factor IIF, beta subunit 21 253 1
PTHR10445:SF0 HMMPANTHER 11 307 1
PTHR10445 HMMPANTHER IPR003196 Transcription initiation factor IIF, beta subunit 11 307 1
1.10.10.10 Gene3D IPR011991 Winged helix-turn-helix DNA-binding domain 190 253 46
SSF46785 SuperFamily 189 254 49
SSF50916 SuperFamily IPR011039 Transcription Factor IIF, Rap30/Rap74, interaction 17 124 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.78 Da
Charge -7.00
Isoelectric point 4.88
Molecular weight 34.62 kDa
Number of residues 307
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
17849during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
17763during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
18474during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
19608during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3533.66during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
18306during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
880.94during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
mtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
pir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
rpb3RNA polymerase II subunit 3 Co-purificationPMID:15620689
Affinity Capture-MSPMID:11839823
rpc10DNA-directed RNA polymerase I, II and III subunit Rpc10 Affinity Capture-WesternPMID:15743411
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
fcp1CTD phosphatase Fcp1 Affinity Capture-MSPMID:11839823
Affinity Capture-WesternPMID:15616156
tfg3transcription factor TFIIF complex subunit Tfg3 Affinity Capture-WesternPMID:15616156
rpb5DNA-directed RNA polymerase I, II and III subunit Rpb5 Affinity Capture-WesternPMID:15743411
External References
Database Identifier Description
NBRP SPBC1198.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1198.13c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1198.13c BioGRID Interaction Datasets
Expression Viewer SPBC1198.13c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1198.13c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1198.13c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1198.13c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1198.13c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1198.13c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1198.13c Cell Cycle Data
GEO SPBC1198.13c GEO profiles
PInt SPBC1198.13c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1198.13c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1198.13c Fission yeast phenotypic data & analysis
SPD / RIKEN16/16H07Orfeome Localization Data
UniProtKB/SwissProtO94424Transcription initiation factor IIF subunit beta
ModBaseO94424Database of comparative protein structure models
STRINGO94424Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595082transcription factor TFIIF complex beta subunit Tfg2 (predicted)
RefSeq mRNANM_001020988972h- transcription factor TFIIF complex beta subunit Tfg2 (predicted) (tfg2), mRNA
European Nucleotide ArchiveCAB91188.1ENA Protein Mapping
UniParcUPI000006BB4AUniProt Archive

Literature for tfg2

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014