alg2 (SPBC11B10.01)


Gene Standard Namealg2 Characterisation Statusbiological_role_inferred
Systematic IDSPBC11B10.01 Feature Typeprotein coding
Synonymspi010, SPACTOKYO_453.27, SPBC32H8.14 Name Description
Productmannosyltransferase complex subunit Alg2 (predicted) Product Size506aa, 57.17 kDa
Genomic Location Chromosome II, 1486094-1489535 (3442nt); CDS:1486152-1487938 (1787nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004378GDP-Man:Man1GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase activityISOSGD:S000003033GO_REF:00000241
GO:0033164glycolipid 6-alpha-mannosyltransferase activityISOSGD:S000003033GO_REF:00000242
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0097502mannosylationISOSGD:S000003033GO_REF:000002424
GO:0006490oligosaccharide-lipid intermediate biosynthetic processISOSGD:S000003033GO_REF:00000245
GO:0006486protein glycosylationIEA UniPathway:UPA00378GO_REF:000004163
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005783endoplasmic reticulumISOSGD:S000003033GO_REF:0000024593
GO:0030176integral component of endoplasmic reticulum membraneNASGO_REF:0000000167
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyalg2ΔNullPECO:0000005, PECO:0000137PMID:236978061331
Microscopyalg2ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002148inviable after spore germination, multiple cell divisions, abnormal morphology185
penetrance FYPO_EXT:0000001Microscopyalg2ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
114860941486236
214862841486400
314864721486925
414870741489535

UTRs

Region Coordinates Reference
five_prime_UTR1486094..1486151PMID:21511999
three_prime_UTR1487939..1489535PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00534 Pfam IPR001296 Glycosyl transferase, family 1 216 289 8
PF00534 Pfam IPR001296 Glycosyl transferase, family 1 305 375 8
TMhelix TMHMM 444 466 959
TMhelix TMHMM 114 136 959
TMhelix TMHMM 82 104 959
TMhelix TMHMM 481 503 959
PTHR12526 HMMPANTHER 1 447 9
PTHR12526:SF221 HMMPANTHER IPR027054 Alpha-1,3/1,6-mannosyltransferase ALG2 1 447 1
3.40.50.2000 Gene3D 220 397 15
SSF53756 SuperFamily 17 422 15

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.98 Da
Charge 19.00
Isoelectric point 9.58
Molecular weight 57.17 kDa
Number of residues 506
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS2PMID:247631071669
present during mitotic M phaseS6PMID:21712547
present during mitotic M phaseS2PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
10016during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
9780during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
8864during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
9700during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
10348during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
13412.07during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
2333.23during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.85during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
CDG-Ii PMID:126845071
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999433
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPBC11B10.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC11B10.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC11B10.01 BioGRID Interaction Datasets
Expression Viewer SPBC11B10.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC11B10.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC11B10.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC11B10.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC11B10.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC11B10.01 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC11B10.01 Cell Cycle Data
GEO SPBC11B10.01 GEO profiles
PInt SPBC11B10.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC11B10.01 Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene2539854mannosyltransferase complex subunit Alg2 (predicted)
EntrezGene2539854mannosyltransferase complex subunit Alg2 (predicted)
SPD / RIKEN49/49C05Orfeome Localization Data
UniProtKB/SwissProtQ96WW6Alpha-1,3/1,6-mannosyltransferase alg2
ModBaseQ96WW6Database of comparative protein structure models
STRINGQ96WW6Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595621mannosyltransferase complex subunit Alg2 (predicted)
RefSeq mRNANM_001021515972h- mannosyltransferase complex subunit Alg2 (predicted) (alg2), mRNA
European Nucleotide ArchiveBAA21387ENA Protein Mapping
European Nucleotide ArchiveBAA21387.2ENA Protein Mapping
European Nucleotide ArchiveCAC37504ENA Protein Mapping
European Nucleotide ArchiveCAC37504.3ENA Protein Mapping
UniParcUPI000006AE20UniProt Archive

Literature for alg2

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014