alg2 (SPBC11B10.01)


Gene Standard Namealg2 Characterisation Statusbiological role inferred
Systematic IDSPBC11B10.01 Feature Typeprotein coding
Synonymspi010, SPACTOKYO_453.27, SPBC32H8.14 Name Description
Productmannosyltransferase complex subunit Alg2 (predicted) Product Size506aa, 57.17 kDa
Genomic Location Chromosome II, 1486094-1489535 (3442nt); CDS:1486152-1487938 (1787nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
macromolecule glycosylation70
Annotation ExtensionEvidenceWith/FromReference
oligosaccharide-lipid intermediate biosynthetic process12
Annotation ExtensionEvidenceWith/FromReference
protein glycosylation70
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
integral component of endoplasmic reticulum membrane68
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationalg2Δ1452

Cell Phenotype

Term NameGenotypesCount
inviable after spore germination, multiple cell divisions, abnormal morphologyalg2Δ205
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1486094..1486236, 1486284..1486400, 1486472..1486925, 1487074..1489535
Intron1486237..1486283, 1486401..1486471, 1486926..1487073
mRNA1486094..1489535
5' UTR1486094..1486151PMID:21511999
CDS1486152..1486236, 1486284..1486400, 1486472..1486925, 1487074..1487938
3' UTR1487939..1489535PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00534 Pfam IPR001296 Glycosyl transferase, family 1 305 374 6
PF00534 Pfam IPR001296 Glycosyl transferase, family 1 219 289 6
TMhelix TMHMM 444 466 953
TMhelix TMHMM 82 104 953
TMhelix TMHMM 114 136 953
TMhelix TMHMM 481 503 953
PTHR12526:SF389 HMMPANTHER 8 290 1
PTHR12526 HMMPANTHER 307 461 8
PTHR12526 HMMPANTHER 8 290 8
PTHR12526:SF389 HMMPANTHER 307 461 1
3.40.50.2000 Gene3D Glycosyltransferase family 28, N-terminal domain 220 397 15
SSF53756 SuperFamily 17 422 15

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.98 Da
Charge 19.00
Codon Adaptation Index 0.39
Isoelectric point 9.58
Molecular weight 57.17 kDa
Number of residues 506
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1677
present during mitotic M phaseS2
present during mitotic M phaseS6
S2
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S2 PMID:21712547
present during mitotic M phase experimental evidence S6 PMID:21712547
experimental evidence S2 PMID:24763107
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for alg2 (SPBC11B10.01)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
10016during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
9780during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8864during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
9700during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
10348during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
13412.07during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2333.23during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.85during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
CDG-Ii PMID:126845071
congenital disorders of glycosylation30
metabolic disorders143
fatty acid metabolism disorders21
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999435
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC11B10.01 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withalg2mannosyltransferase complex subunit Alg2 (predicted) Two-hybridPMID:26771498
External References
Database Identifier Description
NBRP SPBC11B10.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC11B10.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC11B10.01 BioGRID Interaction Datasets
Expression Viewer SPBC11B10.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC11B10.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC11B10.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC11B10.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC11B10.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC11B10.01 Transcriptome Viewer (Bähler Lab)
GEO SPBC11B10.01 GEO profiles
PInt SPBC11B10.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC11B10.01 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC11B10.01 Fission yeast phenotypic data & analysis
Cyclebase SPBC11B10.01.1 Cell Cycle Data
SPD / RIKEN49/49C05Orfeome Localization Data
UniProtKB/SwissProtQ96WW6Alpha-1,3/1,6-mannosyltransferase alg2
ModBaseQ96WW6Database of comparative protein structure models
STRINGQ96WW6Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595621mannosyltransferase complex subunit Alg2 (predicted)
RefSeq mRNANM_001021515972h- mannosyltransferase complex subunit Alg2 (predicted) (alg2), mRNA
European Nucleotide ArchiveBAA21387.2ENA Protein Mapping
European Nucleotide ArchiveCAC37504.3ENA Protein Mapping
UniParcUPI000006AE20UniProt Archive

Literature for alg2

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016