alg2 (SPBC11B10.01)


Gene Standard Namealg2 Characterisation Statusbiological_role_inferred
Systematic IDSPBC11B10.01 Feature Typeprotein coding
Synonymspi010, SPACTOKYO_453.27, SPBC32H8.14 Name Description
Productmannosyltransferase complex subunit Alg2 (predicted) Product Size506aa, 57.17 kDa
Genomic Location Chromosome II, 1486094-1489535 (3442nt); CDS:1486152-1487938 (1787nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
macromolecule glycosylation63
Annotation ExtensionEvidenceWith/FromReference
mannosylation24
Annotation ExtensionEvidenceWith/FromReference
oligosaccharide-lipid intermediate biosynthetic process5
Annotation ExtensionEvidenceWith/FromReference
protein glycosylation63
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
integral component of endoplasmic reticulum membrane68
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationalg2ΔNull1437

Cell Phenotype

Term NameAlleleExpressionCount
inviable after spore germination, multiple cell divisions, abnormal morphologyalg2ΔNull195
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1486094..1486236, 1486284..1486400, 1486472..1486925, 1487074..1489535
mRNA1486094..1489535
5' UTR1486094..1486151PMID:21511999
CDS1486152..1486236, 1486284..1486400, 1486472..1486925, 1487074..1487938
3' UTR1487939..1489535PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00534 Pfam IPR001296 Glycosyl transferase, family 1 305 375 8
PF00534 Pfam IPR001296 Glycosyl transferase, family 1 216 289 8
TMhelix TMHMM 444 466 959
TMhelix TMHMM 114 136 959
TMhelix TMHMM 481 503 959
TMhelix TMHMM 82 104 959
PTHR12526:SF221 HMMPANTHER IPR027054 Alpha-1,3/1,6-mannosyltransferase ALG2 1 447 1
PTHR12526 HMMPANTHER 1 447 9
3.40.50.2000 Gene3D 220 397 15
SSF53756 SuperFamily 17 422 15

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.98 Da
Charge 19.00
Isoelectric point 9.58
Molecular weight 57.17 kDa
Number of residues 506
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS2 1672
present during mitotic M phaseS6
present during mitotic M phaseS2
Annotation ExtensionEvidenceResidueReference
experimental evidence S2 PMID:24763107
present during mitotic M phase experimental evidence S2 PMID:21712547
present during mitotic M phase experimental evidence S6 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
10016during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
9780during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
8864during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
9700during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
13412.07during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
10348during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2333.23during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.85during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
CDG-Ii PMID:126845071
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999433
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPBC11B10.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC11B10.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC11B10.01 BioGRID Interaction Datasets
Expression Viewer SPBC11B10.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC11B10.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC11B10.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC11B10.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC11B10.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC11B10.01 Transcriptome Viewer (Bähler Lab)
GEO SPBC11B10.01 GEO profiles
PInt SPBC11B10.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC11B10.01 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC11B10.01 Fission yeast phenotypic data & analysis
Cyclebase SPBC11B10.01.1 Cell Cycle Data
SPD / RIKEN49/49C05Orfeome Localization Data
UniProtKB/SwissProtQ96WW6Alpha-1,3/1,6-mannosyltransferase alg2
ModBaseQ96WW6Database of comparative protein structure models
STRINGQ96WW6Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595621mannosyltransferase complex subunit Alg2 (predicted)
RefSeq mRNANM_001021515972h- mannosyltransferase complex subunit Alg2 (predicted) (alg2), mRNA
European Nucleotide ArchiveBAA21387.2ENA Protein Mapping
European Nucleotide ArchiveCAC37504.3ENA Protein Mapping
KEGG_Enzyme00510+2.4.1.257+2.4.1.132N-Glycan biosynthesis
KEGG_Enzyme00513+2.4.1.257+2.4.1.132Various types of N-glycan biosynthesis
UniParcUPI000006AE20UniProt Archive
UniPathwayUPA00378Protein modification; protein glycosylation

Literature for alg2

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015