ndc80 (SPBC11C11.03)

Gene Standard Namendc80 Characterisation Statuspublished
Systematic IDSPBC11C11.03 Feature Typeprotein coding
Synonymsndc10, tid3 Name Description
Productspindle pole body protein Ndc80 Product Size624aa, 71.86 kDa
Genomic Location Chromosome II, 3350765-3352972 (2208nt); CDS:3350848-3352814 (1967nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding831
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
condensed nuclear chromosome kinetochore59
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body216
Annotation ExtensionEvidenceWith/FromReference
Ndc80 complex4
Annotation ExtensionEvidenceWith/FromReference
NMS complex11
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000082decreased cell population growth at high temperatureCell growth assayndc80-NH12 (F420S)Not specifiedPECO:0000137PMID:23427262148
FYPO:0002061inviable vegetative cell populationMicroscopyndc80ΔNullPMID:204732891338
Microscopyndc80ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0001387loss of viability at high temperatureCell growth assayndc80-NH12 (F420S)Not specifiedPECO:0000137PMID:2342726220
FYPO:0000091sensitive to thiabendazoleCell growth assayndc80-NH12 (F420S)Not specifiedPECO:0000137, PECO:0000005PMID:23427262176

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001645decreased protein binding38
affecting ndc80 and alp7Western blot assayndc80-NH12 (F420S)Not specifiedPMID:23427262
FYPO:0002902decreased protein localization to kinetochore10
affecting alp7Microscopyndc80-NH12 (F420S)Not specifiedPECO:0000004PMID:23427262
affecting alp14Microscopyndc80-NH12 (F420S)Not specifiedPECO:0000004PMID:23427262
FYPO:0001846increased duration of mitotic anaphase A2
penetrance FYPO_EXT:0000002Microscopyndc80-NH12 (F420S)Not specifiedPECO:0000004PMID:23427262
FYPO:0002429inviable after spore germination, multiple cell divisions, abnormal cell shape, normal cell sizeMicroscopyndc80ΔNullPECO:0000005, PECO:0000137PMID:2369780617
FYPO:0001991inviable after spore germination, without cell divisionMicroscopyndc80ΔNullPECO:0000005, PECO:0000137PMID:23697806474
FYPO:0000228lagging mitotic chromosomes13
penetrance FYPO_EXT:0000003Microscopyndc80-NH12 (F420S)Not specifiedPECO:0000004PMID:23427262
FYPO:0001399normal mitotic spindleMicroscopyndc80-NH12 (F420S)Not specifiedPECO:0000004PMID:2342726210
FYPO:0002901normal protein localization to kinetochore5
affecting ndc80Microscopynot recorded (wild type)OverexpressionPMID:23427262
affecting dis1Microscopyndc80-NH12 (F420S)Not specifiedPECO:0000004PMID:23427262
affecting nuf2Microscopyndc80-NH12 (F420S)Not specifiedPECO:0000004PMID:23427262
affecting spc25Microscopyndc80-NH12 (F420S)Not specifiedPECO:0000004PMID:23427262
Target Of
FYPO affected by mutation in ndc80 spindle pole body protein Ndc80 PMID:23427262
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03801 Pfam IPR005550 Kinetochore protein Ndc80 72 222 1
PTHR10643 HMMPANTHER IPR005550 Kinetochore protein Ndc80 4 623 1
PTHR10643:SF0 HMMPANTHER 4 623 1
Coil ncoils Rabaptin coiled-coil domain 378 399 968
Coil ncoils Rabaptin coiled-coil domain 267 312 968
Coil ncoils Rabaptin coiled-coil domain 484 519 968
Coil ncoils Rabaptin coiled-coil domain 602 623 968
Coil ncoils Rabaptin coiled-coil domain 523 551 968
Coil ncoils Rabaptin coiled-coil domain 326 361 968

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount

Protein Properties

Ave. residue weight 115.16 Da
Charge -13.50
Isoelectric point 5.05
Molecular weight 71.86 kDa
Number of residues 624
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.8during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
spc25kinetochore protein Spc25 Affinity Capture-WesternPMID:15728720
nuf2spindle pole body protein Nuf2 Affinity Capture-MSPMID:16079914
Affinity Capture-WesternPMID:15728720
dis1microtubule-associated protein Dis1 Affinity Capture-WesternPMID:21256022
mis12kinetochore protein Mis12 Affinity Capture-MSPMID:16079914
Affinity Capture-MSPMID:21445296
alp7centrosomal transforming acidic coiled-coil (TACC) protein ortholog Alp7 Reconstituted ComplexPMID:23427262
Affinity Capture-Western
spc7kinetochore protein blinkin, Spc7 Affinity Capture-MSPMID:16079914
mis6inner centromere protein, CENP-I ortholog Mis6 Affinity Capture-MSPMID:21445296
spc24spindle pole body protein Spc24 Two-hybridPMID:15728720
Affinity Capture-Western
Affinity Capture-MSPMID:15371542
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
dis1microtubule-associated protein Dis1 Synthetic LethalityPMID:23427262
Synthetic RescuePMID:21256022
mad2mitotic spindle checkpoint protein Mad2 Phenotypic SuppressionPMID:23427262
alp7centrosomal transforming acidic coiled-coil (TACC) protein ortholog Alp7 Synthetic RescuePMID:23427262
External References
Database Identifier Description
NBRP SPBC11C11.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC11C11.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC11C11.03 BioGRID Interaction Datasets
Expression Viewer SPBC11C11.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC11C11.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC11C11.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC11C11.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC11C11.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC11C11.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC11C11.03 Cell Cycle Data
GEO SPBC11C11.03 GEO profiles
PInt SPBC11C11.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC11C11.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC11C11.03 Fission yeast phenotypic data & analysis
SPD / RIKEN27/27B05Orfeome Localization Data
UniProtKB/SwissProtQ10198Kinetochore protein ndc80
ModBaseQ10198Database of comparative protein structure models
STRINGQ10198Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596392spindle pole body protein Ndc80
RefSeq mRNANM_001022313972h- spindle pole body protein Ndc80 (ndc80), mRNA
European Nucleotide ArchiveCAA20685.1ENA Protein Mapping
UniParcUPI000013A3F2UniProt Archive

Literature for ndc80

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014