ndc80 (SPBC11C11.03)


Gene Standard Namendc80 Characterisation Statuspublished
Systematic IDSPBC11C11.03 Feature Typeprotein coding
Synonymsndc10, tid3 Name Description
ProductNMS complex subunit Ndc80 Product Size624aa, 71.86 kDa
Genomic Location Chromosome II, 3350765-3352972 (2208nt); CDS:3350848-3352814 (1967nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding848
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
chromosome, centromeric region127
Annotation ExtensionEvidenceWith/FromReference
condensed nuclear chromosome kinetochore58
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body216
Annotation ExtensionEvidenceWith/FromReference
NMS complex10
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth at high temperaturendc80-NH12 (F420S)Not specified151
inviable vegetative cell populationndc80ΔNull1427
loss of viability at high temperaturendc80-NH12 (F420S)Not specified35
sensitive to thiabendazolendc80-NH12 (F420S)Not specified185

Cell Phenotype

Term NameAlleleExpressionCount
decreased protein binding51
affecting alp7 and ndc80ndc80-NH12 (F420S)Not specified
decreased protein localization to kinetochore during vegetative growth14
affecting alp7ndc80-NH12 (F420S)Not specified
affecting alp14ndc80-NH12 (F420S)Not specified
increased duration of mitotic anaphase A5
penetrance FYPO_EXT:0000002ndc80-NH12 (F420S)Not specified
inviable after spore germination, multiple cell divisions, abnormal cell shape, normal cell sizendc80ΔNull17
inviable after spore germination, without cell divisionndc80ΔNull485
lagging mitotic chromosomes19
penetrance FYPO_EXT:0000003ndc80-NH12 (F420S)Not specified
normal mitotic spindlendc80-NH12 (F420S)Not specified11
normal protein localization to kinetochore12
affecting nuf2ndc80-NH12 (F420S)Not specified
affecting spc25ndc80-NH12 (F420S)Not specified
affecting dis1ndc80-NH12 (F420S)Not specified
affecting ndc80not recorded (wild type)Overexpression
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in cid12 poly(A) polymerase Cid12 PMID:16738311
FYPO affected by mutation in ndc80 NMS complex subunit Ndc80 PMID:23427262
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
133507653350875
233509253351108
333511523352972

UTRs

Region Coordinates Reference
five_prime_UTR3350765..3350847PMID:21511999
three_prime_UTR3352815..3352972PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03801 Pfam IPR005550 Kinetochore protein Ndc80 72 222 1
PTHR10643:SF2 HMMPANTHER 10 619 1
PTHR10643 HMMPANTHER IPR005550 Kinetochore protein Ndc80 10 619 1
Coil ncoils Rabaptin coiled-coil domain 267 312 971
Coil ncoils Rabaptin coiled-coil domain 378 399 971
Coil ncoils Rabaptin coiled-coil domain 602 623 971
Coil ncoils Rabaptin coiled-coil domain 523 551 971
Coil ncoils Rabaptin coiled-coil domain 326 361 971
Coil ncoils Rabaptin coiled-coil domain 484 519 971

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001080coiled_coil106

Protein Properties

Ave. residue weight 115.16 Da
Charge -13.50
Isoelectric point 5.05
Molecular weight 71.86 kDa
Number of residues 624
Sequence
Gene Expression

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.8during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
spc25NMS complex subunit Spc25 Affinity Capture-WesternPMID:15728720
nuf2NMS complex subunit Nuf2 Affinity Capture-MSPMID:16079914
Two-hybridPMID:15728720
Affinity Capture-Western
dis1microtubule-associated protein Dis1 Affinity Capture-WesternPMID:21256022
mis12kinetochore protein Mis12 Affinity Capture-MSPMID:16079914
Affinity Capture-MSPMID:21445296
alp7centrosomal transforming acidic coiled-coil (TACC) protein ortholog Alp7 Affinity Capture-WesternPMID:23427262
Reconstituted Complex
Protein-peptide
spc7NMS complex subunit, Spc105/KNL-1 family member, blinkin, Spc7 Affinity Capture-MSPMID:16079914
mis6inner centromere protein, CENP-I ortholog Mis6 Affinity Capture-MSPMID:21445296
spc24NMS complex subunit Spc24 Two-hybridPMID:15728720
Affinity Capture-Western
Affinity Capture-MSPMID:15371542
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
dis1microtubule-associated protein Dis1 Synthetic LethalityPMID:23427262
Synthetic RescuePMID:21256022
mad2mitotic spindle checkpoint protein Mad2 Phenotypic SuppressionPMID:23427262
alp7centrosomal transforming acidic coiled-coil (TACC) protein ortholog Alp7 Synthetic RescuePMID:23427262
External References
Database Identifier Description
NBRP SPBC11C11.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC11C11.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC11C11.03 BioGRID Interaction Datasets
Expression Viewer SPBC11C11.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC11C11.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC11C11.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC11C11.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC11C11.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC11C11.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC11C11.03 Cell Cycle Data
GEO SPBC11C11.03 GEO profiles
PInt SPBC11C11.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC11C11.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC11C11.03 Fission yeast phenotypic data & analysis
SPD / RIKEN27/27B05Orfeome Localization Data
UniProtKB/SwissProtQ10198Kinetochore protein ndc80
ModBaseQ10198Database of comparative protein structure models
STRINGQ10198Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596392spindle pole body protein Ndc80
RefSeq mRNANM_001022313972h- spindle pole body protein Ndc80 (ndc80), mRNA
European Nucleotide ArchiveCAA20685.1ENA Protein Mapping
UniParcUPI000013A3F2UniProt Archive

Literature for ndc80

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015