ndc80 (SPBC11C11.03)


Gene Standard Namendc80 Characterisation Statuspublished
Systematic IDSPBC11C11.03 Feature Typeprotein coding
Synonymsndc10, tid3 Name Description
ProductNMS complex subunit Ndc80 Product Size624aa, 71.86 kDa
Genomic Location Chromosome II, 3350765-3352972 (2208nt); CDS:3350848-3352814 (1967nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding868
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
chromosome, centromeric region139
Annotation ExtensionEvidenceWith/FromReference
condensed nuclear chromosome kinetochore60
Annotation ExtensionEvidenceWith/FromReference
condensed nuclear chromosome outer kinetochore13
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body219
Annotation ExtensionEvidenceWith/FromReference
Ndc80 complex4
Annotation ExtensionEvidenceWith/FromReference
NMS complex10
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth at high temperaturendc80-21 (T307A, E379G, L405P)172
ndc80-NH12 (F420S)
increased mitotic indexNdc80Δ371-476 (371-476)2
Ndc80Δ400-476 (400-476)
inviable vegetative cell populationndc80Δ1451
loss of viability at high temperaturendc80-NH12 (F420S)56
ndc80-21 (T307A, E379G, L405P)
sensitive to carbendazimndc80-AK01 (L246P)27
sensitive to thiabendazolendc80-21 (T307A, E379G, L405P)326
ndc80-NH12 (F420S)
ndc80-AK01 (L246P)

Cell Phenotype

Term NameGenotypesCount
abnormal attachment of mitotic spindle microtubules to kinetochorendc80-21 (T307A, E379G, L405P)7
Ndc80-L405P (L405P)
Ndc80Δ371-476 (371-476)
Ndc80Δ400-476 (400-476)
abnormal mitotic sister chromatid segregationNdc80Δ371-476 (371-476)271
Ndc80Δ400-476 (400-476)
abolished mitotic cell cycle spindle assembly checkpointndc80-AK01 (L246P)9
decreased protein binding89
affecting alp7 and ndc80ndc80-NH12 (F420S)
affecting ndc80 and dis1Ndc80Δ371-476 (371-476)
decreased protein localization to kinetochore during mitotic spindle assembly checkpoint signaling1
affects localization of bub3ndc80-AK01 (L246P)
affects localization of mph1ndc80-AK01 (L246P)
affects localization of mad2ndc80-AK01 (L246P)
affects localization of mad1ndc80-AK01 (L246P)
affects localization of bub1ndc80-AK01 (L246P)
affects localization of mad3ndc80-AK01 (L246P)
affects localization of ark1ndc80-AK01 (L246P)
decreased protein localization to kinetochore during vegetative growth18
affects localization of alp7ndc80-NH12 (F420S)
affects localization of dis1ndc80-21 (T307A, E379G, L405P)
affects localization of alp14ndc80-NH12 (F420S)
increased activation of mitotic cell cycle spindle assembly checkpointndc80-21 (T307A, E379G, L405P)16
increased duration of mitotic anaphase Andc80-NH12 (F420S)5
inviable after spore germination, multiple cell divisions, abnormal cell shape, normal cell sizendc80Δ17
inviable after spore germination, without cell divisionndc80Δ495
lagging mitotic chromosomesndc80-NH12 (F420S)37
mitotic metaphase/anaphase transition delayndc80-21 (T307A, E379G, L405P)19
mitotic spindle collapsendc80-21 (T307A, E379G, L405P)9
normal mitotic spindlendc80-NH12 (F420S)17
normal protein localization during vegetative growth250
affecting nuf2ndc80-AK01 (L246P)
affecting spc25ndc80-AK01 (L246P)
normal protein localization to kinetochore during vegetative growth29
affecting nuf2ndc80-NH12 (F420S)
affecting ndc80ndc80+
affecting spc25ndc80-NH12 (F420S)
affecting dis1ndc80-NH12 (F420S)
syntelic kinetochore attachment during mitosisndc80-21 (T307A, E379G, L405P)1

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
inviable vegetative cell populationdis1Δ, ndc80-NH12 (F420S)

Cell Phenotype

Term NameGenotypes
abnormal mitotic cell cycle spindle assembly checkpointndc80-NH12 (F420S), mad2Δ
cutcut7-446, ndc80-AK01 (L246P)
mitotic spindle assembly checkpoint overridecut7-446, ndc80-AK01 (L246P)
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in ndc80 NMS complex subunit Ndc80
FYPO localization affected by mutation in cid12 poly(A) polymerase Cid12
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3350765..3350875, 3350925..3351108, 3351152..3352972
Intron3350876..3350924, 3351109..3351151
mRNA3350765..3352972
5' UTR3350765..3350847PMID:21511999
CDS3350848..3350875, 3350925..3351108, 3351152..3352814
3' UTR3352815..3352972PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03801 Pfam IPR005550 Kinetochore protein Ndc80 72 216 1
PTHR10643 HMMPANTHER IPR005550 Kinetochore protein Ndc80 5 619 1
PTHR10643:SF2 HMMPANTHER 5 619 1
Coil ncoils Predicted coiled-coil protein (DUF2205) 326 360 1057
Coil ncoils Predicted coiled-coil protein (DUF2205) 602 624 1057
Coil ncoils Predicted coiled-coil protein (DUF2205) 378 398 1057
Coil ncoils Predicted coiled-coil protein (DUF2205) 523 550 1057
Coil ncoils Predicted coiled-coil protein (DUF2205) 484 518 1057
Coil ncoils Predicted coiled-coil protein (DUF2205) 267 311 1057

View domain organization at Pfam

Term IDTerm NameReferenceCount
SO:0001080coiled_coil107

Protein Properties

Ave. residue weight 115.16 Da
Charge -13.50
Codon Adaptation Index 0.41
Isoelectric point 5.05
Molecular weight 71.86 kDa
Number of residues 624
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for ndc80 (SPBC11C11.03)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.8during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2506
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC11C11.03 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
forms complex withdis1TOG/XMAP14 microtubule-associated protein Dis1 Reconstituted ComplexPMID:21256022
forms complex withalp7centrosomal transforming acidic coiled-coil (TACC) protein ortholog Alp7 Reconstituted ComplexPMID:23427262
binds to peptidedis1TOG/XMAP14 microtubule-associated protein Dis1 Protein-peptidePMID:21256022
affinity capturesspc24NMS complex subunit Spc24 Affinity Capture-WesternPMID:15728720
affinity capturesnuf2NMS complex subunit Nuf2 Affinity Capture-WesternPMID:15728720
affinity capturesspc25NMS complex subunit Spc25 Affinity Capture-WesternPMID:15728720
affinity capturesdis1TOG/XMAP14 microtubule-associated protein Dis1 Affinity Capture-WesternPMID:21256022
affinity capturesalp7centrosomal transforming acidic coiled-coil (TACC) protein ortholog Alp7 Affinity Capture-WesternPMID:23427262
affinity captured byspc7NMS complex subunit, Spc105/KNL-1 family member, blinkin, Spc7 Affinity Capture-MSPMID:16079914
affinity captured bymis6kinetochore protein, CENP-I ortholog Mis6 Affinity Capture-MSPMID:21445296
affinity captured bymis12NMS complex subunit Mis12 Affinity Capture-MSPMID:21445296
affinity captured byspc24NMS complex subunit Spc24 Affinity Capture-MSPMID:15371542
affinity captured bynuf2NMS complex subunit Nuf2 Affinity Capture-MSPMID:16079914
affinity captured byalp7centrosomal transforming acidic coiled-coil (TACC) protein ortholog Alp7 Affinity Capture-WesternPMID:23427262
binds activation domain construct withspc24NMS complex subunit Spc24 Two-hybridPMID:15728720
binds activation domain construct withnuf2NMS complex subunit Nuf2 Two-hybridPMID:15728720
binds DNA-binding domain construct withnuf2NMS complex subunit Nuf2 Two-hybridPMID:15728720
binds DNA-binding domain construct withspc24NMS complex subunit Spc24 Two-hybridPMID:15728720
(peptide) binds to proteinalp7centrosomal transforming acidic coiled-coil (TACC) protein ortholog Alp7 Protein-peptidePMID:23427262
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC11C11.03 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withdis1TOG/XMAP14 microtubule-associated protein Dis1 Synthetic LethalityPMID:23427262
synthetically rescued bydis1TOG/XMAP14 microtubule-associated protein Dis1 Synthetic RescuePMID:21256022
synthetically rescued byalp7centrosomal transforming acidic coiled-coil (TACC) protein ortholog Alp7 Synthetic RescuePMID:23427262
rescued bymad2mitotic spindle checkpoint protein Mad2 Phenotypic SuppressionPMID:23427262
External References
Database Identifier Description
NBRP SPBC11C11.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC11C11.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC11C11.03 BioGRID Interaction Datasets
Expression Viewer SPBC11C11.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC11C11.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC11C11.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC11C11.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC11C11.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC11C11.03 Transcriptome Viewer (Bähler Lab)
GEO SPBC11C11.03 GEO profiles
PInt SPBC11C11.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC11C11.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC11C11.03 Fission yeast phenotypic data & analysis
Cyclebase SPBC11C11.03.1 Cell Cycle Data
SPD / RIKEN27/27B05Orfeome Localization Data
UniProtKB/SwissProtQ10198Kinetochore protein ndc80
ModBaseQ10198Database of comparative protein structure models
STRINGQ10198Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596392spindle pole body protein Ndc80
RefSeq mRNANM_001022313972h- spindle pole body protein Ndc80 (ndc80), mRNA

Literature for ndc80

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016