ndc80 (SPBC11C11.03)


Gene Standard Namendc80 Characterisation Statuspublished
Systematic IDSPBC11C11.03 Feature Typeprotein coding
Synonymsndc10, tid3 Name Description
ProductNMS complex subunit Ndc80 Product Size624aa, 71.86 kDa
Genomic Location Chromosome II, 3350765-3352972 (2208nt); CDS:3350848-3352814 (1967nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding859
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
chromosome, centromeric region128
Annotation ExtensionEvidenceWith/FromReference
condensed nuclear chromosome kinetochore58
Annotation ExtensionEvidenceWith/FromReference
condensed nuclear chromosome outer kinetochore13
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body216
Annotation ExtensionEvidenceWith/FromReference
Ndc80 complex4
Annotation ExtensionEvidenceWith/FromReference
NMS complex10
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth at high temperaturendc80-21 (T307A, E379G, L405P)Endogenous136
ndc80-NH12 (F420S)Not specified
increased mitotic indexNdc80Δ371-476 (371-476)Endogenous1
Ndc80Δ400-476 (400-476)Endogenous
inviable vegetative cell populationndc80ΔNull1438
loss of viability at high temperaturendc80-21 (T307A, E379G, L405P)Endogenous41
ndc80-NH12 (F420S)Not specified
sensitive to thiabendazolendc80-21 (T307A, E379G, L405P)Endogenous219
ndc80-NH12 (F420S)Not specified

Cell Phenotype

Term NameAlleleExpressionCount
abnormal attachment of mitotic spindle microtubules to kinetochorendc80-21 (T307A, E379G, L405P)Endogenous4
Ndc80-L405P (L405P)Endogenous
Ndc80Δ371-476 (371-476)Endogenous
Ndc80Δ400-476 (400-476)Endogenous
abnormal mitotic sister chromatid segregationNdc80Δ371-476 (371-476)Not specified210
Ndc80Δ400-476 (400-476)Endogenous
decreased protein binding66
affecting alp7 and ndc80ndc80-NH12 (F420S)Not specified
affecting ndc80 and dis1Ndc80Δ371-476 (371-476)Endogenous
decreased protein localization to kinetochore during vegetative growth18
affecting dis1ndc80-21 (T307A, E379G, L405P)Endogenous
affecting alp7ndc80-NH12 (F420S)Not specified
affecting alp14ndc80-NH12 (F420S)Not specified
increased activation of mitotic cell cycle spindle assembly checkpointndc80-21 (T307A, E379G, L405P)Endogenous14
increased duration of mitotic anaphase Andc80-NH12 (F420S)Not specified5
inviable after spore germination, multiple cell divisions, abnormal cell shape, normal cell sizendc80ΔNull17
inviable after spore germination, without cell divisionndc80ΔNull489
lagging mitotic chromosomesndc80-NH12 (F420S)Not specified26
mitotic metaphase/anaphase transition delayndc80-21 (T307A, E379G, L405P)Endogenous13
mitotic spindle collapsendc80-21 (T307A, E379G, L405P)Endogenous7
normal mitotic spindlendc80-NH12 (F420S)Not specified16
normal protein localization to kinetochore25
affecting nuf2ndc80-NH12 (F420S)Not specified
affecting spc25ndc80-NH12 (F420S)Not specified
affecting dis1ndc80-NH12 (F420S)Not specified
affecting ndc80unnamed (wild type)Overexpression
syntelic kinetochore attachment during mitosisndc80-21 (T307A, E379G, L405P)Endogenous1
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in cid12 poly(A) polymerase Cid12
FYPO affected by mutation in ndc80 NMS complex subunit Ndc80
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3350765..3350875, 3350925..3351108, 3351152..3352972
mRNA3350765..3352972
5' UTR3350765..3350847PMID:21511999
CDS3350848..3350875, 3350925..3351108, 3351152..3352814
3' UTR3352815..3352972PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03801 Pfam IPR005550 Kinetochore protein Ndc80 72 222 1
PTHR10643:SF2 HMMPANTHER 10 619 1
PTHR10643 HMMPANTHER IPR005550 Kinetochore protein Ndc80 10 619 1
Coil ncoils Rabaptin coiled-coil domain 484 519 968
Coil ncoils Rabaptin coiled-coil domain 326 361 968
Coil ncoils Rabaptin coiled-coil domain 267 312 968
Coil ncoils Rabaptin coiled-coil domain 523 551 968
Coil ncoils Rabaptin coiled-coil domain 378 399 968
Coil ncoils Rabaptin coiled-coil domain 602 623 968

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001080coiled_coil106

Protein Properties

Ave. residue weight 115.16 Da
Charge -13.50
Isoelectric point 5.05
Molecular weight 71.86 kDa
Number of residues 624
Sequence
Gene Expression

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.8during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesalp7centrosomal transforming acidic coiled-coil (TACC) protein ortholog Alp7 Affinity Capture-WesternPMID:23427262
(peptide) binds to proteinalp7centrosomal transforming acidic coiled-coil (TACC) protein ortholog Alp7 Protein-peptidePMID:23427262
affinity captured byalp7centrosomal transforming acidic coiled-coil (TACC) protein ortholog Alp7 Affinity Capture-WesternPMID:23427262
forms complex withalp7centrosomal transforming acidic coiled-coil (TACC) protein ortholog Alp7 Reconstituted ComplexPMID:23427262
affinity capturesdis1TOG/XMAP14 microtubule-associated protein Dis1 Affinity Capture-WesternPMID:21256022
forms complex withdis1TOG/XMAP14 microtubule-associated protein Dis1 Reconstituted ComplexPMID:21256022
binds to peptidedis1TOG/XMAP14 microtubule-associated protein Dis1 Protein-peptidePMID:21256022
affinity captured bymis12NMS complex subunit Mis12 Affinity Capture-MSPMID:16079914
affinity captured bymis12NMS complex subunit Mis12 Affinity Capture-MSPMID:21445296
affinity captured bymis6inner centromere protein, CENP-I ortholog Mis6 Affinity Capture-MSPMID:21445296
affinity captured bynuf2NMS complex subunit Nuf2 Affinity Capture-MSPMID:16079914
binds DNA-binding domain construct withnuf2NMS complex subunit Nuf2 Two-hybridPMID:15728720
affinity capturesnuf2NMS complex subunit Nuf2 Affinity Capture-WesternPMID:15728720
binds activation domain construct withnuf2NMS complex subunit Nuf2 Two-hybridPMID:15728720
binds DNA-binding domain construct withspc24NMS complex subunit Spc24 Two-hybridPMID:15728720
binds activation domain construct withspc24NMS complex subunit Spc24 Two-hybridPMID:15728720
affinity capturesspc24NMS complex subunit Spc24 Affinity Capture-WesternPMID:15728720
affinity captured byspc24NMS complex subunit Spc24 Affinity Capture-MSPMID:15371542
affinity capturesspc25NMS complex subunit Spc25 Affinity Capture-WesternPMID:15728720
affinity captured byspc7NMS complex subunit, Spc105/KNL-1 family member, blinkin, Spc7 Affinity Capture-MSPMID:16079914
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetically rescued byalp7centrosomal transforming acidic coiled-coil (TACC) protein ortholog Alp7 Synthetic RescuePMID:23427262
synthetic lethal withdis1TOG/XMAP14 microtubule-associated protein Dis1 Synthetic LethalityPMID:23427262
synthetically rescued bydis1TOG/XMAP14 microtubule-associated protein Dis1 Synthetic RescuePMID:21256022
rescued bymad2mitotic spindle checkpoint protein Mad2 Phenotypic SuppressionPMID:23427262
External References
Database Identifier Description
NBRP SPBC11C11.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC11C11.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC11C11.03 BioGRID Interaction Datasets
Expression Viewer SPBC11C11.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC11C11.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC11C11.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC11C11.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC11C11.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC11C11.03 Transcriptome Viewer (Bähler Lab)
GEO SPBC11C11.03 GEO profiles
PInt SPBC11C11.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC11C11.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC11C11.03 Fission yeast phenotypic data & analysis
Cyclebase SPBC11C11.03.1 Cell Cycle Data
SPD / RIKEN27/27B05Orfeome Localization Data
UniProtKB/SwissProtQ10198Kinetochore protein ndc80
ModBaseQ10198Database of comparative protein structure models
STRINGQ10198Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596392spindle pole body protein Ndc80
RefSeq mRNANM_001022313972h- spindle pole body protein Ndc80 (ndc80), mRNA
European Nucleotide ArchiveCAA20685.1ENA Protein Mapping
UniParcUPI000013A3F2UniProt Archive

Literature for ndc80

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015