pob1 (SPBC1289.04c)


Gene Standard Namepob1 Characterisation Statuspublished
Systematic IDSPBC1289.04c Feature Typeprotein coding
Synonyms Name Description
ProductBoi family protein Product Size871aa, 93.77 kDa
Genomic Location Chromosome II, 4391271-4387796 (3476nt); CDS:4391153-4388343 (2811nt)

Ensembl Gene Location
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000920cytokinetic cell separation24
requiredIMPPMID:10436025
GO:0008360regulation of cell shapeIMPPMID:1043602562
GO:0017157regulation of exocytosisIGIcdc42PMID:218996777
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0051285cell cortex of cell tip38
during mitotic interphaseIDAPMID:10436025
GO:0032153cell division siteIDAPMID:16823372292
GO:0051286cell tipIDAPMID:16823372186
GO:0031097medial cortex32
during mitotic cytokinesisIDAPMID:10436025
GO:0016020membraneIEAUniProtKB-KW:KW-0472GO_REF:00000371439
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopypob1ΔNullPECO:0000005, PECO:0000137PMID:236978061309
no_namePMID:10436025
Microscopypob1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002187inviable normal volume spheroid vegetative cellMicroscopypob1ΔNullPECO:0000005, PECO:0000137PMID:2369780648
penetrance FYPO_EXT:0000001
FYPO:0000224lemon-shapedno_namePECO:0000004PMID:104360258
FYPO:0000024stubby cellno_namePECO:0000004PMID:1043602570
FYPO:0000025swollen cellnot recorded (overexpression)PMID:1043602528
Target Of
RelationshipGeneProductReference
regulated by ace2 transcription factor Ace2 PMID:15195092
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
143912714391022
243908264387796

UTRs

Region Start End Reference
three_prime_UTR43883424387796PMID:21511999
five_prime_UTR43912714391154PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00169 Pfam IPR001849 Pleckstrin homology domain 701 806 13
PF00018 Pfam IPR001452 Src homology-3 domain 8 57 18
PF07647 Pfam IPR011510 Sterile alpha motif, type 2 247 311 4
SM00326 SMART IPR001452 Src homology-3 domain 5 64 21
SM00233 SMART IPR001849 Pleckstrin homology domain 699 810 24
SM00454 SMART IPR001660 Sterile alpha motif domain 247 313 5
PS50002 Prosite Profiles IPR001452 Src homology-3 domain 2 65 21
PS50105 Prosite Profiles IPR001660 Sterile alpha motif domain 250 313 4
PS50003 Prosite Profiles IPR001849 Pleckstrin homology domain 698 808 22
PTHR10321 HMMPANTHER 685 833 4
PTHR10321:SF20 HMMPANTHER 685 833 1
G3DSA:2.30.30.40 Gene3D 4 70 21
G3DSA:2.30.29.30 Gene3D IPR011993 698 810 35
G3DSA:1.10.150.50 Gene3D IPR013761 247 311 4
SSF47769 SuperFamily IPR013761 245 314 4
SSF50729 SuperFamily 700 808 36
SSF50044 SuperFamily IPR001452 4 74 21
Low complexity (SEG) seg 857 871
Low complexity (SEG) seg 627 639
Low complexity (SEG) seg 549 560
Low complexity (SEG) seg 456 467
Low complexity (SEG) seg 328 345
Low complexity (SEG) seg 176 188
Low complexity (SEG) seg 88 102
Low complexity (SEG) seg 106 124
Low complexity (SEG) seg 64 75
Low complexity (SEG) seg 35 48

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000251pleckstrin homology domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000251

Protein Properties

Ave. residue weight 107.66 Da
Charge 5.50
Isoelectric point 8.15
Molecular weight 93.77 kDa
Number of residues 871
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residuePMID:195477441192
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
9330.09during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
5070.31during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
4.2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi only603
conserved in fungi4564
conserved in eukaryotes4482
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
exo70exocyst complex subunit Exo70 Synthetic Growth DefectPMID:21401840
ras1GTPase Ras1 Phenotypic SuppressionPMID:24147005
art1arrestin family protein Art1 Dosage RescuePMID:20739711
sec8exocyst complex subunit Sec8 Synthetic Growth DefectPMID:21401840
cdc42Rho family GTPase Cdc42 Dosage RescuePMID:21899677
Phenotypic Suppression
Dosage RescuePMID:21401840
Phenotypic SuppressionPMID:19710424
gap1GTPase activating protein Gap1 Dosage RescuePMID:24147005
rho3Rho family GTPase Rho3 Dosage RescuePMID:21401840
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
for3formin For3 Affinity Capture-WesternPMID:19710424
Two-hybrid
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
cdc42Rho family GTPase Cdc42 Two-hybridPMID:21899677
Two-hybridPMID:21401840
Affinity Capture-WesternPMID:19710424
Two-hybrid
Reconstituted Complex
rho3Rho family GTPase Rho3 Two-hybridPMID:21401840
scd2scaffold protein Scd2 Two-hybridPMID:19710424
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPBC1289.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1289.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1289.04c BioGRID Interaction Datasets
Expression Viewer SPBC1289.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1289.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1289.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1289.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1289.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1289.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1289.04c Cell Cycle Data
GEO SPBC1289.04c GEO profiles
PInt SPBC1289.04c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2539992Boi family protein
EntrezGene2539992Boi family protein
SPD / RIKEN33/33H12Orfeome Localization Data
UniProtKB/SwissProtO74653Protein pob1
ModBaseO74653Database of comparative protein structure models
StringO74653Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596828Boi family protein
RefSeq mRNANM_001023849972h- Boi family protein (pob1), mRNA
European Nucleotide ArchiveBAA33490ENA Protein Mapping
European Nucleotide ArchiveCAB38684ENA Protein Mapping
UniParcUPI0000131D9AUniProt Archive

Literature for pob1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014