pob1 (SPBC1289.04c)


Gene Standard Namepob1 Characterisation Statuspublished
Systematic IDSPBC1289.04c Feature Typeprotein coding
Synonyms Name DescriptionPOmbe Boi
ProductBoi family protein Product Size871aa, 93.77 kDa
Genomic Location Chromosome II, 4391271-4387796 (3476nt); CDS:4391153-4388343 (2811nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
regulation of cell shape66
Annotation ExtensionEvidenceWith/FromReference
regulation of exocytosis7
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell cortex of cell tip44
Annotation ExtensionEvidenceWith/FromReference
cell division site313
Annotation ExtensionEvidenceWith/FromReference
cell tip204
Annotation ExtensionEvidenceWith/FromReference
medial cortex33
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth on glucose carbon sourcepob1+319
Δ N terminus (SH3 and SAM domains deleted)
inviable vegetative cell populationpob1-6641451
Δ C-terminus (PH domain deleted)
pob1Δ
normal growth on glucose carbon sourcepob1-664282

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cycle arrest with replicated DNApob1-66444
abolished protein localization to cell tip, with protein mislocalized to cytoplasm10
affects localization of pob1Δ C-terminus (PH domain deleted)
inviable after spore germination, without cell divisionpob1Δ495
inviable aseptate mononucleate vegetative cell, normal cell lengthpob1-6641
inviable lemon-shaped cellpob1-6642
pob1Δ
inviable normal volume spheroid vegetative cellpob1Δ48
normal protein localization to plasma membrane12
affecting mac1pob1-664
normal protein localization to septum6
affecting mac1pob1-664
spherical vegetative cellpob1+14

FYPO Multi-allele Phenotypes

Cell Phenotype

Term NameGenotypes
meiosis and sporulation in haploidbyr2Δ, pob1+, ras1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO localization affected by mutation in pob1 Boi family protein
GO regulated by ace2 transcription factor Ace2
GO substrate of sst2 human AMSH/STAMBP protein homolog, ubiquitin specific-protease
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
GO substrate of ubp9 ubiquitin C-terminal hydrolase Ubp9
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4391271..4391022, 4390826..4387796
Intron4391021..4390827
mRNA4391271..4387796
5' UTR4391271..4391154PMID:21511999
CDS4391153..4391022, 4390826..4388343
3' UTR4388342..4387796PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00018 Pfam IPR001452 SH3 domain 8 57 20
PF07647 Pfam IPR001660 Sterile alpha motif domain 247 311 4
PF00169 Pfam IPR001849 Pleckstrin homology domain 701 806 12
SM00233 SMART IPR001849 Pleckstrin homology domain 699 810 21
SM00454 SMART IPR001660 Sterile alpha motif domain 247 313 4
SM00326 SMART IPR001452 SH3 domain 5 64 21
PS50002 Prosite Profiles IPR001452 SH3 domain 2 65 21
PS50105 Prosite Profiles IPR001660 Sterile alpha motif domain 250 313 4
PS50003 Prosite Profiles IPR001849 Pleckstrin homology domain 698 808 22
PTHR12844 HMMPANTHER 236 329 1
PTHR12844:SF25 HMMPANTHER 236 329 1
1.10.150.50 Gene3D IPR013761 Sterile alpha motif/pointed domain 247 311 4
2.30.30.40 Gene3D Spectrin repeat 4 70 21
2.30.29.30 Gene3D IPR011993 PH domain-like 698 810 38
SSF47769 SuperFamily IPR013761 Sterile alpha motif/pointed domain 245 314 4
SSF50729 SuperFamily IPR011993 PH domain-like 700 808 36
SSF50044 SuperFamily IPR001452 SH3 domain 4 74 21

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000251pleckstrin homology domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000251

Protein Properties

Ave. residue weight 107.66 Da
Charge 5.50
Codon Adaptation Index 0.40
Isoelectric point 8.15
Molecular weight 93.77 kDa
Number of residues 871
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS668
present during mitotic M phaseS629
present during mitotic M phaseS633
present during mitotic M phaseS433
present during mitotic M phaseS530
present during mitotic M phaseS483
present during mitotic M phaseS839
present during mitotic M phaseS475
present during mitotic M phaseS17
present during mitotic M phaseS637
present during mitotic M phaseS656
present during mitotic M phaseS527
present during mitotic M phaseS652
S17, S483, S629
level fluctuates during mitotic cell cycleS433
level fluctuates during mitotic cell cycleS527
level fluctuates during mitotic cell cycleS530
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S668 PMID:21712547
present during mitotic M phase experimental evidence S629 PMID:21712547
present during mitotic M phase experimental evidence S633 PMID:21712547
present during mitotic M phase experimental evidence S433 PMID:21712547
present during mitotic M phase experimental evidence S530 PMID:21712547
present during mitotic M phase experimental evidence S483 PMID:21712547
present during mitotic M phase experimental evidence S839 PMID:21712547
present during mitotic M phase experimental evidence S475 PMID:21712547
present during mitotic M phase experimental evidence S17 PMID:21712547
present during mitotic M phase experimental evidence S637 PMID:21712547
present during mitotic M phase experimental evidence S656 PMID:21712547
present during mitotic M phase experimental evidence S527 PMID:21712547
present during mitotic M phase experimental evidence S652 PMID:21712547
experimental evidence S17 PMID:24763107
experimental evidence S629 PMID:24763107
level fluctuates during mitotic cell cycle experimental evidence S433 PMID:24763107
level fluctuates during mitotic cell cycle experimental evidence S527 PMID:24763107
level fluctuates during mitotic cell cycle experimental evidence S530 PMID:24763107
experimental evidence S483 PMID:24763107
O-phospho-L-threonine 701
present during mitotic M phaseT649
present during mitotic M phaseT474
T649
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T649 PMID:21712547
present during mitotic M phase experimental evidence T474 PMID:21712547
experimental evidence T649 PMID:24763107
phosphorylated residue 1931
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysineK598, K863 512
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence K863 PMID:26412298
mass spectrometry evidence K598 PMID:26412298
IDA PMID:26412298
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pob1 (SPBC1289.04c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2624during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2588during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2370during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2148during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
9330.09during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2596during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5070.31during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.2during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by S. cerevisiae BOI2 PMID:10436025
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only618
conserved in fungi4606
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC1289.04c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withcdc42Rho family GTPase Cdc42 Two-hybridPMID:19710424
binds DNA-binding domain construct withscd2scaffold protein Scd2 Two-hybridPMID:19710424
binds activation domain construct withrho3Rho family GTPase Rho3 Two-hybridPMID:21401840
binds activation domain construct withcdc42Rho family GTPase Cdc42 Two-hybridPMID:21401840
binds activation domain construct withfor3formin For3 Two-hybridPMID:19710424
affinity captured bycdc42Rho family GTPase Cdc42 Affinity Capture-WesternPMID:19710424
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured byepe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
affinity capturesfor3formin For3 Affinity Capture-WesternPMID:19710424
forms complex withcdc42Rho family GTPase Cdc42 Reconstituted ComplexPMID:19710424
modified bylkh1dual specificity protein kinase Lkh1 Biochemical ActivityPMID:26167880
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC1289.04c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
overexpression rescuesgap1GTPase activating protein Gap1 Dosage RescuePMID:24147005
overexpression rescuescdc42Rho family GTPase Cdc42 Dosage RescuePMID:21899677
overexpression rescuesart1arrestin family protein Art1 Dosage RescuePMID:20739711
synthetic growth defect withsec8exocyst complex subunit Sec8 Synthetic Growth DefectPMID:21401840
synthetic growth defect withexo70exocyst complex subunit Exo70 Synthetic Growth DefectPMID:21401840
rescued by overexpression ofrho3Rho family GTPase Rho3 Dosage RescuePMID:21401840
rescued by overexpression ofcdc42Rho family GTPase Cdc42 Dosage RescuePMID:21401840
rescuesras1GTPase Ras1 Phenotypic SuppressionPMID:24147005
rescuescdc42Rho family GTPase Cdc42 Phenotypic SuppressionPMID:19710424
External References
Database Identifier Description
NBRP SPBC1289.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1289.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1289.04c BioGRID Interaction Datasets
Expression Viewer SPBC1289.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1289.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1289.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1289.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1289.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1289.04c Transcriptome Viewer (Bähler Lab)
GEO SPBC1289.04c GEO profiles
PInt SPBC1289.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1289.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1289.04c Fission yeast phenotypic data & analysis
Cyclebase SPBC1289.04c.1 Cell Cycle Data
SPD / RIKEN33/33H12Orfeome Localization Data
UniProtKB/SwissProtO74653Protein pob1
ModBaseO74653Database of comparative protein structure models
STRINGO74653Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596828Boi family protein
RefSeq mRNANM_001023849972h- Boi family protein (pob1), mRNA

Literature for pob1

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016