pob1 (SPBC1289.04c)


Gene Standard Namepob1 Characterisation Statuspublished
Systematic IDSPBC1289.04c Feature Typeprotein coding
Synonyms Name DescriptionPOmbe Boi
ProductBoi family protein Product Size871aa, 93.77 kDa
Genomic Location Chromosome II, 4391271-4387796 (3476nt); CDS:4391153-4388343 (2811nt)

Ensembl Gene Location
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0008360regulation of cell shapeIMPPMID:1043602562
GO:0017157regulation of exocytosisIGIcdc42PMID:218996777
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0051285cell cortex of cell tip42
during mitotic interphaseIDAPMID:10436025
GO:0032153cell division siteIDAPMID:16823372298
IDAPMID:12206652
GO:0051286cell tipIDAPMID:12206652192
IDAPMID:16823372
GO:0031097medial cortex33
during mitotic cytokinesisIDAPMID:10436025
GO:0016020membraneIEAUniProtKB-KW:KW-0472GO_REF:00000371450
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaypob1+ (wild type)OverexpressionPECO:0000126, PECO:0000102PMID:10436025119
Cell growth assayΔ N terminus (SH3 and SAM domains deleted)Not specifiedPMID:10436025
FYPO:0002061inviable vegetative cell populationMicroscopypob1ΔNullPMID:204732891337
Cell growth assayΔ C-terminus (PH domain deleted)Not specifiedPMID:10436025
Microscopypob1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Cell growth assaypob1-664Not specifiedPECO:0000004PMID:10436025
FYPO:0001164normal growth on glucose carbon sourceCell growth assaypob1-664Not specifiedPECO:0000005PMID:10436025165

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000444abnormal mitotic cell cycle arrest with replicated DNAFlow cytometry datapob1-664Not specifiedPECO:0000004PMID:1043602526
FYPO:0000443abnormal protein localization during vegetative growth217
affecting pob1MicroscopyΔ C-terminus (PH domain deleted)Not specifiedPMID:10436025
FYPO:0001991inviable after spore germination, without cell divisionMicroscopypob1ΔNullPMID:10436025473
FYPO:0003501inviable aseptate mononucleate vegetative cell, normal cell lengthMicroscopypob1-664Not specifiedPECO:0000004PMID:104360251
FYPO:0003333inviable lemon-shaped cellMicroscopypob1-664Not specifiedPECO:0000004PMID:104360251
Microscopypob1ΔNullPMID:10436025
FYPO:0002187inviable normal volume spheroid vegetative cell49
penetrance FYPO_EXT:0000001Microscopypob1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0002674normal protein localization to plasma membrane5
affecting mac1Microscopypob1-664Not specifiedPECO:0000229PMID:12206652
FYPO:0003414normal protein localization to septum4
affecting mac1Microscopypob1-664Not specifiedPECO:0000229PMID:12206652
FYPO:0000129spherical vegetative cellMicroscopypob1+ (wild type)OverexpressionPMID:104360253
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in mac1 membrane anchored protein Mac1 PMID:12206652
FYPO affected by mutation in pob1 Boi family protein PMID:10436025
GO regulated by ace2 transcription factor Ace2 PMID:15195092
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
143912714391022
243908264387796
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07647 Pfam IPR011510 Sterile alpha motif, type 2 247 311 4
PF00018 Pfam IPR001452 Src homology-3 domain 8 57 18
PF00169 Pfam IPR001849 Pleckstrin homology domain 701 806 12
SM00326 SMART IPR001452 Src homology-3 domain 5 64 21
SM00233 SMART IPR001849 Pleckstrin homology domain 699 810 21
SM00454 SMART IPR001660 Sterile alpha motif domain 247 313 4
PS50105 Prosite Profiles IPR001660 Sterile alpha motif domain 250 313 4
PS50003 Prosite Profiles IPR001849 Pleckstrin homology domain 698 808 22
PS50002 Prosite Profiles IPR001452 Src homology-3 domain 2 65 21
PTHR10321 HMMPANTHER 685 833 4
PTHR10321:SF20 HMMPANTHER 685 833 1
1.10.150.50 Gene3D IPR013761 Sterile alpha motif/pointed domain 247 311 4
2.30.30.40 Gene3D 4 70 21
2.30.29.30 Gene3D IPR011993 Pleckstrin homology-like domain 698 810 38
SSF50044 SuperFamily IPR001452 Src homology-3 domain 4 74 21
SSF50729 SuperFamily 700 808 36
SSF47769 SuperFamily IPR013761 Sterile alpha motif/pointed domain 245 314 4

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000251pleckstrin homology domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000251

Protein Properties

Ave. residue weight 107.66 Da
Charge 5.50
Isoelectric point 8.15
Molecular weight 93.77 kDa
Number of residues 871
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS530PMID:217125471670
present during mitotic M phase
present during mitotic M phaseS475PMID:21712547
S629PMID:24763107
present during mitotic M phaseS668PMID:21712547
present during mitotic M phaseS17PMID:21712547
present during mitotic M phaseS629PMID:21712547
present during mitotic M phaseS633PMID:21712547
present during mitotic M phaseS637PMID:21712547
present during mitotic M phaseS483PMID:21712547
present during mitotic M phaseS433PMID:21712547
present during mitotic M phaseS527PMID:21712547
S483PMID:24763107
S17PMID:24763107
level fluctuates during mitotic cell cycleS433PMID:24763107
level fluctuates during mitotic cell cycleS530PMID:24763107
level fluctuates during mitotic cell cycleS527PMID:24763107
present during mitotic M phaseS652PMID:21712547
present during mitotic M phaseS839PMID:21712547
present during mitotic M phaseS656PMID:21712547
MOD:00047O-phospho-L-threonineT649PMID:21712547690
present during mitotic M phase
present during mitotic M phaseT474PMID:21712547
T649PMID:24763107
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2624during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2588during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2370during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2148during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
9330.09during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2596during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
5070.31during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by S. cerevisiae BOI2 PMID:10436025
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only620
conserved in fungi4599
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
exo70exocyst complex subunit Exo70 Synthetic Growth DefectPMID:21401840
ras1GTPase Ras1 Phenotypic SuppressionPMID:24147005
art1arrestin family protein Art1 Dosage RescuePMID:20739711
sec8exocyst complex subunit Sec8 Synthetic Growth DefectPMID:21401840
cdc42Rho family GTPase Cdc42 Phenotypic SuppressionPMID:21899677
Dosage Rescue
Dosage RescuePMID:21401840
Phenotypic SuppressionPMID:19710424
gap1GTPase activating protein Gap1 Dosage RescuePMID:24147005
rho3Rho family GTPase Rho3 Dosage RescuePMID:21401840
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
for3formin For3 Affinity Capture-WesternPMID:19710424
Two-hybrid
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
cdc42Rho family GTPase Cdc42 Two-hybridPMID:21899677
Two-hybridPMID:21401840
Affinity Capture-WesternPMID:19710424
Reconstituted Complex
Two-hybrid
rho3Rho family GTPase Rho3 Two-hybridPMID:21401840
scd2scaffold protein Scd2 Two-hybridPMID:19710424
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPBC1289.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1289.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1289.04c BioGRID Interaction Datasets
Expression Viewer SPBC1289.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1289.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1289.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1289.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1289.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1289.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1289.04c Cell Cycle Data
GEO SPBC1289.04c GEO profiles
PInt SPBC1289.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1289.04c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN33/33H12Orfeome Localization Data
UniProtKB/SwissProtO74653Protein pob1
ModBaseO74653Database of comparative protein structure models
STRINGO74653Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596828Boi family protein
RefSeq mRNANM_001023849972h- Boi family protein (pob1), mRNA
European Nucleotide ArchiveBAA33490.1ENA Protein Mapping
European Nucleotide ArchiveCAB38684.1ENA Protein Mapping
UniParcUPI0000131D9AUniProt Archive

Literature for pob1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014