pob1 (SPBC1289.04c)


Gene Standard Namepob1 Characterisation Statuspublished
Systematic IDSPBC1289.04c Feature Typeprotein coding
Synonyms Name DescriptionPOmbe Boi
ProductBoi family protein Product Size871aa, 93.77 kDa
Genomic Location Chromosome II, 4391271-4387796 (3476nt); CDS:4391153-4388343 (2811nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
regulation of cell shape74
Annotation ExtensionEvidenceWith/FromReference
regulation of exocytosis7
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell cortex of cell tip44
Annotation ExtensionEvidenceWith/FromReference
cell division site305
Annotation ExtensionEvidenceWith/FromReference
cell tip194
Annotation ExtensionEvidenceWith/FromReference
medial cortex33
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth on glucose carbon sourceΔ N terminus (SH3 and SAM domains deleted)Not specified247
pob1+ (wild type)Overexpression
inviable vegetative cell populationΔ C-terminus (PH domain deleted)Not specified1428
pob1-664Not specified
pob1ΔNull
normal growth on glucose carbon sourcepob1-664Not specified200

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cycle arrest with replicated DNApob1-664Not specified35
abolished protein localization to cell tip, with protein mislocalized to cytoplasm9
affecting pob1Δ C-terminus (PH domain deleted)Not specified
inviable after spore germination, without cell divisionpob1ΔNull481
inviable aseptate mononucleate vegetative cell, normal cell lengthpob1-664Not specified1
inviable lemon-shaped cellpob1-664Not specified2
pob1ΔNull
inviable normal volume spheroid vegetative cellpob1ΔNull49
normal protein localization to plasma membrane6
affecting mac1pob1-664Not specified
normal protein localization to septum4
affecting mac1pob1-664Not specified
spherical vegetative cellpob1+ (wild type)Overexpression13
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in pob1 Boi family protein PMID:10436025
GO regulated by ace2 transcription factor Ace2 PMID:15195092
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4391271..4391022, 4390826..4387796
mRNA4391271..4387796
5' UTR4391271..4391154PMID:21511999
CDS4391153..4391022, 4390826..4388343
3' UTR4388342..4387796PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00018 Pfam IPR001452 SH3 domain 8 57 18
PF07647 Pfam IPR011510 Sterile alpha motif, type 2 247 311 4
PF00169 Pfam IPR001849 Pleckstrin homology domain 701 806 12
SM00454 SMART IPR001660 Sterile alpha motif domain 247 313 4
SM00233 SMART IPR001849 Pleckstrin homology domain 699 810 21
SM00326 SMART IPR001452 SH3 domain 5 64 21
PS50105 Prosite Profiles IPR001660 Sterile alpha motif domain 250 313 4
PS50002 Prosite Profiles IPR001452 SH3 domain 2 65 21
PS50003 Prosite Profiles IPR001849 Pleckstrin homology domain 698 808 22
PTHR10321 HMMPANTHER 1 849 4
PTHR10321:SF29 HMMPANTHER IPR030508 Boi1/Boi2 1 849 1
2.30.29.30 Gene3D IPR011993 Pleckstrin homology-like domain 698 810 38
1.10.150.50 Gene3D IPR013761 Sterile alpha motif/pointed domain 247 311 4
2.30.30.40 Gene3D 4 70 21
SSF50729 SuperFamily 700 808 36
SSF50044 SuperFamily IPR001452 SH3 domain 4 74 21
SSF47769 SuperFamily IPR013761 Sterile alpha motif/pointed domain 245 314 4

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000251pleckstrin homology domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000251

Protein Properties

Ave. residue weight 107.66 Da
Charge 5.50
Isoelectric point 8.15
Molecular weight 93.77 kDa
Number of residues 871
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS17, S483, S629 1672
level fluctuates during mitotic cell cycleS433
present during mitotic M phaseS475
level fluctuates during mitotic cell cycleS530
present during mitotic M phaseS530
level fluctuates during mitotic cell cycleS527
present during mitotic M phaseS17
present during mitotic M phaseS656
present during mitotic M phaseS652
present during mitotic M phaseS629
present during mitotic M phaseS433
present during mitotic M phaseS483
present during mitotic M phaseS668
present during mitotic M phaseS839
present during mitotic M phaseS527
present during mitotic M phaseS637
present during mitotic M phaseS633
Annotation ExtensionEvidenceResidueReference
experimental evidence S17 PMID:24763107
present during mitotic M phase experimental evidence S17 PMID:21712547
present during mitotic M phase experimental evidence S433 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence S433 PMID:24763107
present during mitotic M phase experimental evidence S475 PMID:21712547
experimental evidence S483 PMID:24763107
present during mitotic M phase experimental evidence S483 PMID:21712547
present during mitotic M phase experimental evidence S527 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence S527 PMID:24763107
present during mitotic M phase experimental evidence S530 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence S530 PMID:24763107
experimental evidence S629 PMID:24763107
present during mitotic M phase experimental evidence S629 PMID:21712547
present during mitotic M phase experimental evidence S633 PMID:21712547
present during mitotic M phase experimental evidence S637 PMID:21712547
present during mitotic M phase experimental evidence S652 PMID:21712547
present during mitotic M phase experimental evidence S656 PMID:21712547
present during mitotic M phase experimental evidence S668 PMID:21712547
present during mitotic M phase experimental evidence S839 PMID:21712547
O-phospho-L-threonine 698
present during mitotic M phaseT649
present during mitotic M phaseT474
T649
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T474 PMID:21712547
experimental evidence T649 PMID:24763107
present during mitotic M phase experimental evidence T649 PMID:21712547
phosphorylated residue 1926
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2624during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2588during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2370during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2148during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2596during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
9330.09during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
5070.31during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by S. cerevisiae BOI2 PMID:10436025
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only620
conserved in fungi4604
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
binds activation domain construct withcdc42Rho family GTPase Cdc42 Two-hybridPMID:21899677
binds activation domain construct withcdc42Rho family GTPase Cdc42 Two-hybridPMID:21401840
binds DNA-binding domain construct withcdc42Rho family GTPase Cdc42 Two-hybridPMID:19710424
affinity captured bycdc42Rho family GTPase Cdc42 Affinity Capture-WesternPMID:19710424
forms complex withcdc42Rho family GTPase Cdc42 Reconstituted ComplexPMID:19710424
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
affinity captured byepe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
affinity capturesfor3formin For3 Affinity Capture-WesternPMID:19710424
binds activation domain construct withfor3formin For3 Two-hybridPMID:19710424
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
binds activation domain construct withrho3Rho family GTPase Rho3 Two-hybridPMID:21401840
binds DNA-binding domain construct withscd2scaffold protein Scd2 Two-hybridPMID:19710424
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
overexpression rescuesart1arrestin family protein Art1 Dosage RescuePMID:20739711
overexpression rescuescdc42Rho family GTPase Cdc42 Dosage RescuePMID:21899677
rescuescdc42Rho family GTPase Cdc42 Phenotypic SuppressionPMID:21899677
rescued by overexpression ofcdc42Rho family GTPase Cdc42 Dosage RescuePMID:21401840
rescuescdc42Rho family GTPase Cdc42 Phenotypic SuppressionPMID:19710424
synthetic growth defect withexo70exocyst complex subunit Exo70 Synthetic Growth DefectPMID:21401840
overexpression rescuesgap1GTPase activating protein Gap1 Dosage RescuePMID:24147005
rescuesras1GTPase Ras1 Phenotypic SuppressionPMID:24147005
rescued by overexpression ofrho3Rho family GTPase Rho3 Dosage RescuePMID:21401840
synthetic growth defect withsec8exocyst complex subunit Sec8 Synthetic Growth DefectPMID:21401840
External References
Database Identifier Description
NBRP SPBC1289.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1289.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1289.04c BioGRID Interaction Datasets
Expression Viewer SPBC1289.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1289.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1289.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1289.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1289.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1289.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1289.04c Cell Cycle Data
GEO SPBC1289.04c GEO profiles
PInt SPBC1289.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1289.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1289.04c Fission yeast phenotypic data & analysis
SPD / RIKEN33/33H12Orfeome Localization Data
UniProtKB/SwissProtO74653Protein pob1
ModBaseO74653Database of comparative protein structure models
STRINGO74653Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596828Boi family protein
RefSeq mRNANM_001023849972h- Boi family protein (pob1), mRNA
European Nucleotide ArchiveBAA33490.1ENA Protein Mapping
European Nucleotide ArchiveCAB38684.1ENA Protein Mapping
UniParcUPI0000131D9AUniProt Archive

Literature for pob1

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015