tim21 (SPBC1289.09)


Gene Standard Nametim21 Characterisation Statusbiological_role_inferred
Systematic IDSPBC1289.09 Feature Typeprotein coding
Synonyms Name DescriptionTranslocase of the Inner Membrane
ProductTIM23 translocase complex subunit Tim21 (predicted) Product Size223aa, 25.57 kDa
Genomic Location Chromosome II, 4399249-4400401 (1153nt); CDS:4399320-4399991 (672nt)

Ensembl Gene Location
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030150protein import into mitochondrial matrixISOSGD:S000003265GO_REF:000002417
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371018
GO:0005744mitochondrial inner membrane presequence translocase complexISOSGD:S000003265GO_REF:000002411
GO:0005739mitochondrionIDAPMID:16823372758
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002620normal growth on trichostatin ACell growth assaytim21ΔNullPECO:0000005, PECO:0000201, PECO:0000142PMID:23861937110
FYPO:0002617sensitive to sodium butyrate93
expressivity FYPO_EXT:0000002Cell growth assaytim21ΔNullPECO:0000201, PECO:0000005, PECO:0000142PMID:23861937
FYPO:0000115sensitive to valproic acid155
expressivity FYPO_EXT:0000001Cell growth assaytim21ΔNullPECO:0000201, PECO:0000005, PECO:0000142PMID:23861937
FYPO:0002060viable vegetative cell populationMicroscopytim21ΔNullPMID:204732893759
Microscopytim21ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopytim21ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
143992494400401

UTRs

Region Coordinates Reference
five_prime_UTR4399249..4399319PMID:21511999
three_prime_UTR4399992..4400401PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08294 Pfam IPR013261 Mitochondrial import inner membrane translocase subunit Tim21 62 199 1
TMhelix TMHMM 70 92 959
PTHR13032 HMMPANTHER IPR013261 Mitochondrial import inner membrane translocase subunit Tim21 1 221 1
PTHR13032:SF1 HMMPANTHER 1 221 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.66 Da
Charge 15.50
Isoelectric point 10.27
Molecular weight 25.57 kDa
Number of residues 223
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
29257during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
25724during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
25317during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
25217during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
26097during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
4263.44during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2656.07during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6.1during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
SPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:22681890
kin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
SPBC1604.16cRNA-binding protein, G-patch type (predicted) Negative GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
prp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Positive GeneticPMID:22681890
hus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
whi5cell cycle transcriptional repressor Whi5 (predicted) Negative GeneticPMID:22681890
bcp1thioredoxin peroxidase Bcp1 Negative GeneticPMID:22681890
msa1RNA-binding protein Msa1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC1289.09 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1289.09 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1289.09 BioGRID Interaction Datasets
Expression Viewer SPBC1289.09 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1289.09 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1289.09 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1289.09 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1289.09 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1289.09 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1289.09 Cell Cycle Data
GEO SPBC1289.09 GEO profiles
PInt SPBC1289.09 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1289.09 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1289.09 Fission yeast phenotypic data & analysis
SPD / RIKEN07/07G04Orfeome Localization Data
UniProtKB/SwissProtO94618Mitochondrial import inner membrane translocase subunit tim21
ModBaseO94618Database of comparative protein structure models
STRINGO94618Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596833TIM23 translocase complex subunit Tim21 (predicted)
RefSeq mRNANM_001023854972h- TIM23 translocase complex subunit Tim21 (predicted) (tim21), mRNA
European Nucleotide ArchiveCAB38689.1ENA Protein Mapping
UniParcUPI000006ABAAUniProt Archive

Literature for tim21

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014