tim21 (SPBC1289.09)

Gene Standard Nametim21 Characterisation Statusbiological_role_inferred
Systematic IDSPBC1289.09 Feature Typeprotein coding
Synonyms Name DescriptionTranslocase of the Inner Membrane
ProductTIM23 translocase complex subunit Tim21 (predicted) Product Size223aa, 25.57 kDa
Genomic Location Chromosome II, 4399249-4400401 (1153nt); CDS:4399320-4399991 (672nt)

Ensembl Gene Location
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030150protein import into mitochondrial matrixISOSGD:S000003265GO_REF:000002419
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371016
GO:0005744mitochondrial inner membrane presequence translocase complexISOSGD:S000003265GO_REF:000002411
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002620normal cell population growth on trichostatin ACell growth assaytim21ΔNullPECO:0000005, PECO:0000201, PECO:0000142PMID:23861937110
FYPO:0002617sensitive to sodium butyrateCell growth assaytim21ΔNullPECO:0000201, PECO:0000005, PECO:0000142PMID:2386193793
expressivity FYPO_EXT:0000002
FYPO:0000115sensitive to valproic acidCell growth assaytim21ΔNullPECO:0000201, PECO:0000005, PECO:0000142PMID:23861937154
expressivity FYPO_EXT:0000001
FYPO:0002060viable vegetative cell populationMicroscopytim21ΔNullPMID:204732893755
Microscopytim21ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopytim21ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Start End Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08294 Pfam IPR013261 Mitochondrial import inner membrane translocase subunit Tim21 62 199 1
TMhelix TMHMM 70 92 959
PTHR13032:SF1 HMMPANTHER 1 221 1
PTHR13032 HMMPANTHER IPR013261 Mitochondrial import inner membrane translocase subunit Tim21 1 221 1
Low complexity (SEG) seg 69 87

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.66 Da
Charge 15.50
Isoelectric point 10.27
Molecular weight 25.57 kDa
Number of residues 223
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2656.07during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
4263.44during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
6.1during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
1.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
SPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:22681890
kin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
SPBC1604.16cRNA-binding protein, G-patch type (predicted) Negative GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
prp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Positive GeneticPMID:22681890
hus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
whi5cell cycle transcriptional repressor Whi5 (predicted) Negative GeneticPMID:22681890
SPBC1773.02cthioredoxin peroxidase (predicted) Negative GeneticPMID:22681890
msa1RNA-binding protein Msa1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC1289.09 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1289.09 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1289.09 BioGRID Interaction Datasets
Expression Viewer SPBC1289.09 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1289.09 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1289.09 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1289.09 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1289.09 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1289.09 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1289.09 Cell Cycle Data
GEO SPBC1289.09 GEO profiles
PInt SPBC1289.09 Protein-Protein Interaction Predictor (Bähler Lab)
SPD / RIKEN07/07G04Orfeome Localization Data
EntrezGene2539981TIM23 translocase complex subunit Tim21 (predicted)
WikiGene2539981TIM23 translocase complex subunit Tim21 (predicted)
UniProtKB/SwissProtO94618Mitochondrial import inner membrane translocase subunit tim21
ModBaseO94618Database of comparative protein structure models
StringO94618Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596833TIM23 translocase complex subunit Tim21 (predicted)
RefSeq mRNANM_001023854972h- TIM23 translocase complex subunit Tim21 (predicted) (tim21), mRNA
European Nucleotide ArchiveCAB38689ENA Protein Mapping
UniParcUPI000006ABAAUniProt Archive

Literature for tim21

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014