adn2 (SPBC1289.10c)


Gene Standard Nameadn2 Characterisation Statusbiological_role_inferred
Systematic IDSPBC1289.10c Feature Typeprotein coding
Synonyms Name DescriptionADhesioN defective
Producttranscription factor (predicted) Product Size743aa, 81.16 kDa
Genomic Location Chromosome II, 4402885-4400383 (2503nt); CDS:4402847-4400616 (2232nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
GO Cellular Component
Term NameCount
cytoplasm4206
Annotation ExtensionEvidenceWith/FromReference
nucleus2697
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
sensitive to hydroxyureaadn2ΔNull525
sensitive to methyl methanesulfonateadn2ΔNull227
viable vegetative cell populationadn2ΔNull3783

Cell Phenotype

Term NameAlleleExpressionCount
abolished cell-substrate adhesionadn2ΔNull9
normal volume spheroid vegetative celladn2ΔNull55
pseudohyphal growth abolishedadn2ΔNull12
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
144028854400383

UTRs

Region Coordinates Reference
five_prime_UTR4402885..4402848PMID:21511999
three_prime_UTR4400615..4400383PMID:21511999
exon4402847..4400616
mRNA4402885..4400383
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08513 Pfam IPR013720 LisH dimerisation motif, subgroup 41 66 8
PF04503 Pfam 156 700 1
SM00667 SMART IPR006594 LisH dimerisation motif 38 70 10
PS50896 Prosite Profiles IPR006594 LisH dimerisation motif 38 70 11
PTHR12610 HMMPANTHER 7 186 2
Coil ncoils Rabaptin coiled-coil domain 250 271 968
Coil ncoils Rabaptin coiled-coil domain 451 472 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.23 Da
Charge 6.50
Isoelectric point 7.21
Molecular weight 81.16 kDa
Number of residues 743
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS318
present during mitotic M phaseS519
present during mitotic M phaseS314
present during mitotic M phaseS483
S89, S175, S519
present during mitotic M phaseS485
present during mitotic M phaseS175
present during mitotic M phaseS310
present during mitotic M phaseS89
Annotation ExtensionEvidenceResidueReference
experimental evidence S89 PMID:24763107
present during mitotic M phase experimental evidence S89 PMID:21712547
experimental evidence S175 PMID:24763107
present during mitotic M phase experimental evidence S175 PMID:21712547
present during mitotic M phase experimental evidence S310 PMID:21712547
present during mitotic M phase experimental evidence S314 PMID:21712547
present during mitotic M phase experimental evidence S318 PMID:21712547
present during mitotic M phase experimental evidence S483 PMID:21712547
present during mitotic M phase experimental evidence S485 PMID:21712547
experimental evidence S519 PMID:24763107
present during mitotic M phase experimental evidence S519 PMID:21712547
phosphorylated residue 1923
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
898during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1289during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1007during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1226during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1326during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1626.65during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.5during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi only619
conserved in fungi4604
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Positive GeneticPMID:22681890
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
negative genetic interaction withatp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withbdc1bromodomain containing protein 1, Bdc1 Negative GeneticPMID:22681890
positive genetic interaction withbzz1diacylglycerol binding protein Bzz1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
negative genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
negative genetic interaction withdad1DASH complex subunit Dad1 Negative GeneticPMID:22681890
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
negative genetic interaction withdad3DASH complex subunit Dad3 Negative GeneticPMID:22681890
negative genetic interaction withdam1DASH complex subunit Dam1 Negative GeneticPMID:22681890
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
negative genetic interaction withelp3elongator complex subunit Elp3 (predicted) Negative GeneticPMID:22681890
rescued bygsf2galactose-specific flocculin Gsf2 Phenotypic SuppressionPMID:23236291
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction withint6eIF3e subunit Int6 Negative GeneticPMID:22681890
positive genetic interaction withknd1Cullin-associated NEDD8-dissociated protein Knd1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withlub1WD repeat protein Lub1 Negative GeneticPMID:22681890
negative genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
negative genetic interaction withppm1leucine carboxyl methyltransferase Ppm1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
positive genetic interaction withpyp1tyrosine phosphatase Pyp1 Positive GeneticPMID:22681890
negative genetic interaction withrad55RecA family ATPase Rad55/Rhp55 Negative GeneticPMID:22681890
negative genetic interaction withraf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Negative GeneticPMID:22681890
negative genetic interaction withraf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
negative genetic interaction withreb1RNA polymerase I transcription termination factor/ RNA polymerase II transcription factor Reb1 Negative GeneticPMID:22681890
negative genetic interaction withrik1silencing protein Rik1 Negative GeneticPMID:22681890
negative genetic interaction withrpb9DNA-directed RNA polymerase II complex subunit Rpb9 Negative GeneticPMID:22681890
negative genetic interaction withrsv2transcription factor Rsv2 Negative GeneticPMID:22681890
negative genetic interaction withsap10splicing factor 3B Negative GeneticPMID:22681890
positive genetic interaction withsap114U2 snRNP subunit Sap114 Positive GeneticPMID:22681890
negative genetic interaction withsif1Sad1 interacting factor 1 Negative GeneticPMID:22681890
negative genetic interaction withsnf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
positive genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPBC1778.03cNADH pyrophosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withspc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
positive genetic interaction withSPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
negative genetic interaction withsts5RNB-like protein Negative GeneticPMID:22681890
negative genetic interaction withtif38translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
negative genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtsc1hamartin Negative GeneticPMID:22681890
negative genetic interaction withtsc2tuberin Negative GeneticPMID:22681890
negative genetic interaction withubp2ubiquitin C-terminal hydrolase Ubp2 Negative GeneticPMID:22681890
positive genetic interaction withusp102U1 snRNP-associated protein Usp102 Positive GeneticPMID:22681890
negative genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC1289.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1289.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1289.10c BioGRID Interaction Datasets
Expression Viewer SPBC1289.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1289.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1289.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1289.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1289.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1289.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1289.10c Cell Cycle Data
GEO SPBC1289.10c GEO profiles
PInt SPBC1289.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1289.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1289.10c Fission yeast phenotypic data & analysis
SPD / RIKEN38/38B02Orfeome Localization Data
UniProtKB/SwissProtO94619Adhesion defective protein 2
ModBaseO94619Database of comparative protein structure models
STRINGO94619Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596834transcription factor (predicted)
RefSeq mRNANM_001023855972h- transcription factor (predicted) (adn2), mRNA
European Nucleotide ArchiveCAB38690.1ENA Protein Mapping
UniParcUPI0000069FF7UniProt Archive

Literature for adn2

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015