adn2 (SPBC1289.10c)


Gene Standard Nameadn2 Characterisation Statusbiological_role_inferred
Systematic IDSPBC1289.10c Feature Typeprotein coding
Synonyms Name DescriptionADhesioN defective
Producttranscription factor (predicted) Product Size743aa, 81.16 kDa
Genomic Location Chromosome II, 4402885-4400383 (2503nt); CDS:4402847-4400616 (2232nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000978RNA polymerase II core promoter proximal region sequence-specific DNA bindingISOSGD:S000000911GO_REF:000002494
GO:0000982RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activityISOSGD:S000000911GO_REF:000002491
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0010811positive regulation of cell-substrate adhesionIMPPMID:195423127
GO:0045944positive regulation of transcription from RNA polymerase II promoterISOSGD:S000000911GO_REF:000002481
GO:0006366transcription from RNA polymerase II promoterISOSGD:S000000911GO_REF:0000024139
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmIDAPMID:168233724204
GO:0005634nucleusISOSGD:S000000911GO_REF:00000242737
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000088sensitive to hydroxyureaCell growth assayadn2ΔNullPECO:0000137, PECO:0000005PMID:23173672513
FYPO:0000089sensitive to methyl methanesulfonateCell growth assayadn2ΔNullPECO:0000137, PECO:0000005PMID:23173672214
FYPO:0002060viable vegetative cell populationMicroscopyadn2ΔNullPMID:204732893751
Microscopyadn2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000010abolished cell-substrate adhesionadn2ΔNullPMID:195423129
FYPO:0000946normal volume spheroid vegetative cell55
penetrance FYPO_EXT:0000001Microscopyadn2ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0000551pseudohyphal growth abolishedMicroscopyadn2ΔNullPMID:1954231212
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
144028854400383
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04503 Pfam 156 700 1
PF08513 Pfam IPR013720 LisH dimerisation motif, subgroup 41 66 8
SM00667 SMART IPR006594 LisH dimerisation motif 38 70 10
PS50896 Prosite Profiles IPR006594 LisH dimerisation motif 38 70 11
PTHR12610 HMMPANTHER 1 223 2
PTHR12610:SF12 HMMPANTHER 1 223 2
Coil ncoils Rabaptin coiled-coil domain 250 271 968
Coil ncoils Rabaptin coiled-coil domain 451 472 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.23 Da
Charge 6.50
Isoelectric point 7.21
Molecular weight 81.16 kDa
Number of residues 743
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS519PMID:217125471670
present during mitotic M phase
present during mitotic M phaseS318PMID:21712547
present during mitotic M phaseS89PMID:21712547
present during mitotic M phaseS310PMID:21712547
present during mitotic M phaseS314PMID:21712547
present during mitotic M phaseS483PMID:21712547
present during mitotic M phaseS485PMID:21712547
S175PMID:24763107
S89PMID:24763107
present during mitotic M phaseS175PMID:21712547
S519PMID:24763107
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
898during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1289during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1007during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1226during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1626.65during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1326during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.5during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi only620
conserved in fungi4599
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ams2cell cycle regulated GATA-type transcription factor Ams2 Positive GeneticPMID:22681890
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
lub1WD repeat protein Lub1 Negative GeneticPMID:22681890
dad1DASH complex subunit Dad1 Negative GeneticPMID:22681890
bdc1bromodomain containing protein 1, Bdc1 Negative GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
pyp1tyrosine phosphatase Pyp1 Positive GeneticPMID:22681890
usp102U1 snRNP-associated protein Usp102 Positive GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
elp3elongator complex subunit Elp3 (predicted) Negative GeneticPMID:22681890
SPAC821.05translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
rad55RecA family ATPase Rad55/Rhp55 Negative GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
raf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
gsf2galactose-specific flocculin Gsf2 Phenotypic SuppressionPMID:23236291
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
rik1silencing protein Rik1 Negative GeneticPMID:22681890
spc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
ubp2ubiquitin C-terminal hydrolase Ubp2 Negative GeneticPMID:22681890
dad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
dad3DASH complex subunit Dad3 Negative GeneticPMID:22681890
sap10splicing factor 3B Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
rsv2transcription factor Rsv2 Negative GeneticPMID:22681890
atp11F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:22681890
tom70mitochondrial TOM complex subunit Tom70 (predicted) Negative GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
ypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
SPBC1778.03cNADH pyrophosphatase (predicted) Negative GeneticPMID:22681890
sts5RNB-like protein Negative GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
tsc1hamartin Negative GeneticPMID:22681890
rpb9DNA-directed RNA polymerase II complex subunit Rpb9 Negative GeneticPMID:22681890
sif1Sad1 interacting factor 1 Negative GeneticPMID:22681890
bzz1diacylglycerol binding protein Bzz1 (predicted) Positive GeneticPMID:22681890
reb1RNA polymerase I transcription termination factor Reb1 Negative GeneticPMID:22681890
raf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Negative GeneticPMID:22681890
int6eIF3e subunit Int6 Negative GeneticPMID:22681890
SPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
ppm1leucine carboxyl methyltransferase Ppm1 (predicted) Negative GeneticPMID:22681890
sap114U2 snRNP subunit Sap114 Positive GeneticPMID:22681890
knd1Cullin-associated NEDD8-dissociated protein Knd1 (predicted) Positive GeneticPMID:22681890
SPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
dam1DASH complex subunit Dam1 Negative GeneticPMID:22681890
tsc2tuberin Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC1289.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1289.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1289.10c BioGRID Interaction Datasets
Expression Viewer SPBC1289.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1289.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1289.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1289.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1289.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1289.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1289.10c Cell Cycle Data
GEO SPBC1289.10c GEO profiles
PInt SPBC1289.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1289.10c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN38/38B02Orfeome Localization Data
UniProtKB/SwissProtO94619Adhesion defective protein 2
ModBaseO94619Database of comparative protein structure models
STRINGO94619Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596834transcription factor (predicted)
RefSeq mRNANM_001023855972h- transcription factor (predicted) (adn2), mRNA
European Nucleotide ArchiveCAB38690.1ENA Protein Mapping
UniParcUPI0000069FF7UniProt Archive

Literature for adn2

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014