pst1 (SPBC12C2.10c)


Gene Standard Namepst1 Characterisation Statuspublished
Systematic IDSPBC12C2.10c Feature Typeprotein coding
SynonymsSPBC21D10.01c Name Description
ProductClr6 histone deacetylase complex subunit Pst1 Product Size1522aa, 171.46 kDa
Genomic Location Chromosome II, 2452041-2457119 (5079nt); CDS:2452052-2456757 (4706nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding851
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
chromatin remodeling157
Annotation ExtensionEvidenceWith/FromReference
chromatin silencing at centromere61
Annotation ExtensionEvidenceWith/FromReference
histone deacetylation26
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Clr6 histone deacetylase complex I''12
Annotation ExtensionEvidenceWith/FromReference
nuclear pericentric heterochromatin36
Annotation ExtensionEvidenceWith/FromReference
nucleus2697
Annotation ExtensionEvidenceWith/FromReference
Rpd3L complex5
Annotation ExtensionEvidenceWith/FromReference
Rpd3S complex16
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationpst1ΔNull1428
sensitive to trichostatin Apst1-1 (Y1307->stop)Not specified42

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclepst1ΔNull808
decreased transpositionpst1-1 (Y1307->stop)Not specified1
decreased transposon-derived cDNA recombinationpst1-1 (Y1307->stop)Not specified1
increased pseudohyphal growthpst1-1 (Y1307->stop)Not specified2
inviable after spore germination with elongated germ tubepst1ΔNull163
normal level of transposon-derived cDNA in cellpst1-1 (Y1307->stop)Not specified1
normal level of transposon-encoded protein in cellpst1-1 (Y1307->stop)Not specified1
normal mitotic recombination6
affecting ade6pst1-1 (Y1307->stop)Not specified
normal transposon gene mRNA levelpst1-1 (Y1307->stop)Not specified1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
124520412452181
224523192457119

UTRs

Region Coordinates Reference
five_prime_UTR2452041..2452051PMID:21511999
three_prime_UTR2456758..2457119PMID:21511999
mRNA2452041..2457119
exon2452052..2452181,2452319..2456757
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08295 Pfam IPR013194 Histone deacetylase interacting 592 692 3
PF02671 Pfam IPR003822 Paired amphipathic helix 200 246 3
PF02671 Pfam IPR003822 Paired amphipathic helix 529 572 3
PF02671 Pfam IPR003822 Paired amphipathic helix 367 413 3
SM00761 SMART IPR013194 Histone deacetylase interacting 592 693 3
PS51477 Prosite Profiles IPR003822 Paired amphipathic helix 504 576 3
PS51477 Prosite Profiles IPR003822 Paired amphipathic helix 345 415 3
PS51477 Prosite Profiles IPR003822 Paired amphipathic helix 178 248 3
PTHR12346:SF0 HMMPANTHER 1 1328 3
PTHR12346 HMMPANTHER 1 1328 3
1g1eB00 Gene3D IPR003822 Paired amphipathic helix 175 247 3
1g1eB00 Gene3D IPR003822 Paired amphipathic helix 514 571 3
1g1eB00 Gene3D IPR003822 Paired amphipathic helix 344 414 3
SSF47762 SuperFamily IPR003822 Paired amphipathic helix 342 414 3
SSF47762 SuperFamily IPR003822 Paired amphipathic helix 508 576 3
SSF47762 SuperFamily IPR003822 Paired amphipathic helix 175 247 3
Coil ncoils Rabaptin coiled-coil domain 645 666 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001382SIN3 family co-repressorPMID:10022921Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001382

Protein Properties

Ave. residue weight 112.65 Da
Charge -13.00
Isoelectric point 5.98
Molecular weight 171.46 kDa
Number of residues 1522
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS1436
present during mitotic M phaseS902
present during mitotic M phaseS904
present during mitotic M phaseS442
present during mitotic M phaseS330
S1434, S1436, S1443, S1474, S1476
present during mitotic M phaseS444
present during mitotic M phaseS1443
present during mitotic M phaseS1476
present during mitotic M phaseS434
present during mitotic M phaseS1434
present during mitotic M phaseS903
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S330 PMID:21712547
present during mitotic M phase experimental evidence S434 PMID:21712547
present during mitotic M phase experimental evidence S442 PMID:21712547
present during mitotic M phase experimental evidence S444 PMID:21712547
present during mitotic M phase experimental evidence S902 PMID:21712547
present during mitotic M phase experimental evidence S903 PMID:21712547
present during mitotic M phase experimental evidence S904 PMID:21712547
experimental evidence S1434 PMID:24763107
present during mitotic M phase experimental evidence S1434 PMID:21712547
experimental evidence S1436 PMID:24763107
present during mitotic M phase experimental evidence S1436 PMID:21712547
experimental evidence S1443 PMID:24763107
present during mitotic M phase experimental evidence S1443 PMID:21712547
experimental evidence S1474 PMID:24763107
experimental evidence S1476 PMID:24763107
present during mitotic M phase experimental evidence S1476 PMID:21712547
O-phospho-L-threonine 694
present during mitotic M phaseT438
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T438 PMID:21712547
phosphorylated residue 1923
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3148during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3052during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2914during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3064during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3371during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2293.14during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
4970.92during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity capturesclr6histone deacetylase (class I) Clr6 Affinity Capture-WesternPMID:12526748
affinity captured byclr6histone deacetylase (class I) Clr6 Affinity Capture-WesternPMID:12526748
affinity captured bynts1Schizosaccharomyces specific protein Affinity Capture-MSPMID:25002536
External References
Database Identifier Description
NBRP SPBC12C2.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC12C2.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC12C2.10c BioGRID Interaction Datasets
Expression Viewer SPBC12C2.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC12C2.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC12C2.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC12C2.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC12C2.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC12C2.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC12C2.10c Cell Cycle Data
GEO SPBC12C2.10c GEO profiles
PInt SPBC12C2.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC12C2.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC12C2.10c Fission yeast phenotypic data & analysis
SPD / RIKEN48/48G08Orfeome Localization Data
UniProtKB/SwissProtQ09750Paired amphipathic helix protein pst1
ModBaseQ09750Database of comparative protein structure models
STRINGQ09750Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596012Clr6 histone deacetylase complex subunit Pst1
RefSeq mRNANM_001021920972h- Clr6 histone deacetylase complex subunit Pst1 (pst1), mRNA
European Nucleotide ArchiveAAF90180.1ENA Protein Mapping
European Nucleotide ArchiveCAA20757.1ENA Protein Mapping
UniParcUPI0000132819UniProt Archive

Literature for pst1

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015