pst1 (SPBC12C2.10c)


Gene Standard Namepst1 Characterisation Statuspublished
Systematic IDSPBC12C2.10c Feature Typeprotein coding
SynonymsSPBC21D10.01c Name Description
ProductClr6 histone deacetylase complex subunit Pst1 Product Size1522aa, 171.46 kDa
Genomic Location Chromosome II, 2452041-2457119 (5079nt); CDS:2452052-2456757 (4706nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding863
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
chromatin remodeling156
Annotation ExtensionEvidenceWith/FromReference
chromatin silencing at centromere59
Annotation ExtensionEvidenceWith/FromReference
histone deacetylation26
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Clr6 histone deacetylase complex I''12
Annotation ExtensionEvidenceWith/FromReference
nuclear pericentric heterochromatin42
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
Rpd3L complex5
Annotation ExtensionEvidenceWith/FromReference
Rpd3S complex16
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationpst1Δ1453
sensitive to trichostatin Apst1-1 (Y1307->stop)44

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cyclepst1Δ858
decreased transpositionpst1-1 (Y1307->stop)1
decreased transposon-derived cDNA recombinationpst1-1 (Y1307->stop)1
increased pseudohyphal growthpst1-1 (Y1307->stop)2
inviable after spore germination with elongated germ tubepst1Δ164
normal level of transposon-derived cDNA in cellpst1-1 (Y1307->stop)1
normal level of transposon-encoded protein in cellpst1-1 (Y1307->stop)1
normal mitotic recombination7
affecting ade6pst1-1 (Y1307->stop)
normal transposon gene mRNA levelpst1-1 (Y1307->stop)1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2452041..2452181, 2452319..2457119
Intron2452182..2452318
5' UTR2452041..2452051PMID:21511999
3' UTR2456758..2457119PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF16879 Pfam IPR031693 Sin3, C-terminal 1004 1282 3
PF08295 Pfam IPR013194 Histone deacetylase interacting domain 594 688 3
PF02671 Pfam IPR003822 Paired amphipathic helix 201 245 2
PF02671 Pfam IPR003822 Paired amphipathic helix 368 412 2
SM00761 SMART IPR013194 Histone deacetylase interacting domain 592 693 3
PS51477 Prosite Profiles IPR003822 Paired amphipathic helix 504 576 3
PS51477 Prosite Profiles IPR003822 Paired amphipathic helix 178 248 3
PS51477 Prosite Profiles IPR003822 Paired amphipathic helix 345 415 3
PTHR12346:SF0 HMMPANTHER 48 908 3
PTHR12346:SF0 HMMPANTHER 939 1327 3
PTHR12346 HMMPANTHER 48 908 3
PTHR12346 HMMPANTHER 939 1327 3
1g1eB00 Gene3D IPR003822 Paired amphipathic helix 514 571 3
1g1eB00 Gene3D IPR003822 Paired amphipathic helix 175 247 3
1g1eB00 Gene3D IPR003822 Paired amphipathic helix 344 414 3
SSF47762 SuperFamily IPR003822 Paired amphipathic helix 342 414 3
SSF47762 SuperFamily IPR003822 Paired amphipathic helix 175 247 3
SSF47762 SuperFamily IPR003822 Paired amphipathic helix 508 576 3
Coil ncoils Predicted coiled-coil protein (DUF2205) 645 665 1057
Coil ncoils Predicted coiled-coil protein (DUF2205) 1519 1522 1057

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001382SIN3 family co-repressorPMID:10022921Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001382

Protein Properties

Ave. residue weight 112.65 Da
Charge -13.00
Codon Adaptation Index 0.41
Isoelectric point 5.98
Molecular weight 171.46 kDa
Number of residues 1522
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS904
present during mitotic M phaseS434
present during mitotic M phaseS1436
present during mitotic M phaseS1443
present during mitotic M phaseS444
present during mitotic M phaseS903
present during mitotic M phaseS1476
present during mitotic M phaseS1434
present during mitotic M phaseS330
present during mitotic M phaseS902
present during mitotic M phaseS442
S1434, S1436, S1443, S1474, S1476
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S904 PMID:21712547
present during mitotic M phase experimental evidence S434 PMID:21712547
present during mitotic M phase experimental evidence S1436 PMID:21712547
present during mitotic M phase experimental evidence S1443 PMID:21712547
present during mitotic M phase experimental evidence S444 PMID:21712547
present during mitotic M phase experimental evidence S903 PMID:21712547
present during mitotic M phase experimental evidence S1476 PMID:21712547
present during mitotic M phase experimental evidence S1434 PMID:21712547
present during mitotic M phase experimental evidence S330 PMID:21712547
present during mitotic M phase experimental evidence S902 PMID:21712547
present during mitotic M phase experimental evidence S442 PMID:21712547
experimental evidence S1436 PMID:24763107
experimental evidence S1443 PMID:24763107
experimental evidence S1476 PMID:24763107
experimental evidence S1474 PMID:24763107
experimental evidence S1434 PMID:24763107
O-phospho-L-threonine 700
present during mitotic M phaseT438
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T438 PMID:21712547
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pst1 (SPBC12C2.10c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3148during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3052during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2914during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3064during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2293.14during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3371during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4970.92during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.2during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC12C2.10c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withphp4CCAAT-binding factor complex subunit Php4 Two-hybridPMID:26771498
affinity captured bynts1Clr6 histone deacetylase complex subunit Nts1 Affinity Capture-MSPMID:25002536
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured byclr6histone deacetylase (class I) Clr6 Affinity Capture-WesternPMID:12526748
affinity capturesclr6histone deacetylase (class I) Clr6 Affinity Capture-WesternPMID:12526748
External References
Database Identifier Description
NBRP SPBC12C2.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC12C2.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC12C2.10c BioGRID Interaction Datasets
Expression Viewer SPBC12C2.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC12C2.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC12C2.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC12C2.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC12C2.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC12C2.10c Transcriptome Viewer (Bähler Lab)
GEO SPBC12C2.10c GEO profiles
PInt SPBC12C2.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC12C2.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC12C2.10c Fission yeast phenotypic data & analysis
Cyclebase SPBC12C2.10c.1 Cell Cycle Data
SPD / RIKEN48/48G08Orfeome Localization Data
UniProtKB/SwissProtQ09750Paired amphipathic helix protein pst1
ModBaseQ09750Database of comparative protein structure models
STRINGQ09750Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596012Clr6 histone deacetylase complex subunit Pst1
RefSeq mRNANM_001021920972h- Clr6 histone deacetylase complex subunit Pst1 (pst1), mRNA

Literature for pst1

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016