pst1 (SPBC12C2.10c)


Gene Standard Namepst1 Characterisation Statuspublished
Systematic IDSPBC12C2.10c Feature Typeprotein coding
SynonymsSPBC21D10.01c Name Description
ProductClr6 histone deacetylase complex subunit Pst1 Product Size1522aa, 171.46 kDa
Genomic Location Chromosome II, 2452041-2457119 (5079nt); CDS:2452052-2456757 (4706nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005515protein bindingIPIclr6PMID:12526748831
GO:0003714transcription corepressor activityISOSGD:S000005364PMID:1556532212
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0007049cell cycleIEAUniProtKB-KW:KW-0131GO_REF:0000037959
GO:0006338chromatin remodelingISOSGD:S000005364GO_REF:0000024160
GO:0030702chromatin silencing at centromereIMPPMID:1252674856
GO:0016575histone deacetylationIGIclr6PMID:1252674823
GO:1903507negative regulation of nucleic acid-templated transcriptionISOSGD:S000005364PMID:15565322169
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0033698Rpd3L complexIDAPMID:174501515
GO:0032221Rpd3S complexIDAPMID:1277339216
GO:0031618nuclear pericentric heterochromatinIDAPMID:1252674835
GO:0005634nucleusIDAPMID:100229212740
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopypst1ΔNullPECO:0000005, PECO:0000137PMID:236978061338
Cell growth assaypst1ΔNullPECO:0000004PMID:10022921
Cell growth assaypst1ΔNullPECO:0000005PMID:10022921
Microscopypst1ΔNullPMID:20473289
FYPO:0002546sensitive to trichostatin ACell growth assaypst1-1 (Y1307->stop)Not specifiedPMID:1002292141

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopypst1ΔNullPECO:0000005, PECO:0000137PMID:23697806686
FYPO:0000593decreased transpositionCell growth assaypst1-1 (Y1307->stop)Not specifiedPMID:100229211
FYPO:0003775decreased transposon-derived cDNA recombinationCell growth assaypst1-1 (Y1307->stop)Not specifiedPMID:100229211
FYPO:0003776increased pseudohyphal growthMicroscopypst1-1 (Y1307->stop)Not specifiedPECO:0000268PMID:100229212
FYPO:0000314inviable after spore germination with elongated germ tube163
penetrance FYPO_EXT:0000001Microscopypst1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0003774normal level of transposon-derived cDNA in cellGel electrophoresis evidencepst1-1 (Y1307->stop)Not specifiedPMID:100229211
FYPO:0003773normal level of transposon-encoded protein in cellWestern blot assaypst1-1 (Y1307->stop)Not specifiedPMID:100229211
FYPO:0000503normal mitotic recombination4
affecting ade6Cell growth assaypst1-1 (Y1307->stop)Not specifiedPMID:10022921
FYPO:0003772normal transposon gene mRNA levelTranscript expression level evidencepst1-1 (Y1307->stop)Not specifiedPMID:100229211
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
124520412452181
224523192457119

UTRs

Region Coordinates Reference
five_prime_UTR2452041..2452051PMID:21511999
three_prime_UTR2456758..2457119PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08295 Pfam IPR013194 Histone deacetylase interacting 592 692 3
PF02671 Pfam IPR003822 Paired amphipathic helix 529 572 3
PF02671 Pfam IPR003822 Paired amphipathic helix 200 246 3
PF02671 Pfam IPR003822 Paired amphipathic helix 367 413 3
SM00761 SMART IPR013194 Histone deacetylase interacting 592 693 3
PS51477 Prosite Profiles IPR003822 Paired amphipathic helix 178 248 3
PS51477 Prosite Profiles IPR003822 Paired amphipathic helix 345 415 3
PS51477 Prosite Profiles IPR003822 Paired amphipathic helix 504 576 3
PTHR12346 HMMPANTHER 18 1327 3
1g1eB00 Gene3D IPR003822 Paired amphipathic helix 514 571 3
1g1eB00 Gene3D IPR003822 Paired amphipathic helix 175 247 3
1g1eB00 Gene3D IPR003822 Paired amphipathic helix 344 414 3
SSF47762 SuperFamily IPR003822 Paired amphipathic helix 342 414 3
SSF47762 SuperFamily IPR003822 Paired amphipathic helix 508 576 3
SSF47762 SuperFamily IPR003822 Paired amphipathic helix 175 247 3
Coil ncoils Rabaptin coiled-coil domain 645 666 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001382SIN3 family co-repressorPMID:10022921Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001382

Protein Properties

Ave. residue weight 112.65 Da
Charge -13.00
Isoelectric point 5.98
Molecular weight 171.46 kDa
Number of residues 1522
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS1436PMID:247631071670
present during mitotic M phaseexperimental evidenceS903PMID:21712547
experimental evidenceS1434PMID:24763107
present during mitotic M phaseexperimental evidenceS442PMID:21712547
experimental evidenceS1476PMID:24763107
experimental evidenceS1474PMID:24763107
present during mitotic M phaseexperimental evidenceS904PMID:21712547
present during mitotic M phaseexperimental evidenceS330PMID:21712547
present during mitotic M phaseexperimental evidenceS902PMID:21712547
present during mitotic M phaseexperimental evidenceS1443PMID:21712547
experimental evidenceS1443PMID:24763107
present during mitotic M phaseexperimental evidenceS434PMID:21712547
present during mitotic M phaseexperimental evidenceS1476PMID:21712547
present during mitotic M phaseexperimental evidenceS444PMID:21712547
present during mitotic M phaseexperimental evidenceS1436PMID:21712547
present during mitotic M phaseexperimental evidenceS1434PMID:21712547
MOD:00047O-phospho-L-threonineexperimental evidenceT438PMID:21712547692
present during mitotic M phase
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3148during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3052during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2914during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3064during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3371during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2293.14during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
4970.92during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
nts1Schizosaccharomyces specific protein Affinity Capture-MSPMID:25002536
clr6histone deacetylase (class I) Clr6 Affinity Capture-WesternPMID:12526748
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
External References
Database Identifier Description
NBRP SPBC12C2.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC12C2.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC12C2.10c BioGRID Interaction Datasets
Expression Viewer SPBC12C2.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC12C2.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC12C2.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC12C2.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC12C2.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC12C2.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC12C2.10c Cell Cycle Data
GEO SPBC12C2.10c GEO profiles
PInt SPBC12C2.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC12C2.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC12C2.10c Fission yeast phenotypic data & analysis
SPD / RIKEN48/48G08Orfeome Localization Data
UniProtKB/SwissProtQ09750Paired amphipathic helix protein pst1
ModBaseQ09750Database of comparative protein structure models
STRINGQ09750Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596012Clr6 histone deacetylase complex subunit Pst1
RefSeq mRNANM_001021920972h- Clr6 histone deacetylase complex subunit Pst1 (pst1), mRNA
European Nucleotide ArchiveAAF90180.1ENA Protein Mapping
European Nucleotide ArchiveCAA20757.1ENA Protein Mapping
UniParcUPI0000132819UniProt Archive

Literature for pst1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014