glo1 (SPBC12C2.12c)


Gene Standard Nameglo1 Characterisation Statuspublished
Systematic IDSPBC12C2.12c Feature Typeprotein coding
SynonymsSPBC21D10.03c Name Description
Productglyoxalase I Product Size302aa, 34.41 kDa
Genomic Location Chromosome II, 2446543-2447857 (1315nt); CDS:2446698-2447606 (909nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004462lactoylglutathione lyase activityIDAPMID:150422801
heterologous_systemIGISGD:S000004463PMID:15042280
GO:0008270zinc ion bindingIDAPMID:15042280248
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0071470cellular response to osmotic stressTASPMID:1571429544
GO:0006749glutathione metabolic processIDAPMID:1504228013
GO:0019243methylglyoxal catabolic process to D-lactateIMPPMID:150422803
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISOSGD:S000004463GO_REF:00000244198
GO:0005829cytosolIDAPMID:168233722317
GO:0005634nucleusISOSGD:S000004463GO_REF:00000242730
IDAPMID:16823372
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyglo1ΔNullPECO:0000005, PECO:0000137PMID:236978063755
Microscopyglo1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopyglo1ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
124465432447857

UTRs

Region Start End Reference
three_prime_UTR24476072447857PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00903 Pfam IPR004360 Glyoxalase/fosfomycin resistance/dioxygenase domain 11 150 1
PF00903 Pfam IPR004360 Glyoxalase/fosfomycin resistance/dioxygenase domain 167 298 1
PS00934 Prosite Patterns IPR018146 Glyoxalase I, conserved site 169 189 1
PS00934 Prosite Patterns IPR018146 Glyoxalase I, conserved site 14 35 1
PS00935 Prosite Patterns IPR018146 Glyoxalase I, conserved site 241 258 1
PS00935 Prosite Patterns IPR018146 Glyoxalase I, conserved site 93 110 1
PTHR10374 HMMPANTHER 6 299 1
PTHR10374:SF0 HMMPANTHER 6 299 1
G3DSA:3.10.180.10 Gene3D 5 154 2
G3DSA:3.10.180.10 Gene3D 155 301 2
SSF54593 SuperFamily 6 158 2
SSF54593 SuperFamily 164 300 2
TIGR00068 tigrfam IPR004361 Glyoxalase I 7 158 1
TIGR00068 tigrfam IPR004361 Glyoxalase I 160 302 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.94 Da
Charge -2.00
Isoelectric point 6.19
Molecular weight 34.41 kDa
Number of residues 302
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
43192.49during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
11869.61during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
3.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
monomeric PMID:150422806
Catalytic Activity Attributes
DescriptionQualifierReferenceCount
k(cat)/ K(m)m 4.3x10(7) M(-1) min(-1) PMID:150422801
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3394
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
dad1DASH complex subunit Dad1 Negative GeneticPMID:22681890
SPAC1A6.03cphospholipase (predicted) Positive GeneticPMID:22681890
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
cut2securin, sister chromatid separation inhibitor Negative GeneticPMID:22681890
php3CCAAT-binding factor complex subunit Php3 Negative GeneticPMID:22681890
SPBC1348.10cphospholipase (predicted) Negative GeneticPMID:22681890
vps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
php5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
cph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
mph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
atg6beclin family protein, involved in autophagy Negative GeneticPMID:22681890
pst2Clr6 histone deacetylase complex subunit Pst2 Negative GeneticPMID:22681890
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC12C2.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC12C2.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC12C2.12c BioGRID Interaction Datasets
Expression Viewer SPBC12C2.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC12C2.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC12C2.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC12C2.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC12C2.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC12C2.12c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC12C2.12c Cell Cycle Data
GEO SPBC12C2.12c GEO profiles
PInt SPBC12C2.12c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2539736glyoxalase I
EntrezGene2539736glyoxalase I
SPD / RIKEN12/12E09Orfeome Localization Data
IntEnz4.4.1.5Integrated relational Enzyme database
Rhea4.4.1.5Annotated reactions database
UniProtKB/SwissProtQ09751Lactoylglutathione lyase
ModBaseQ09751Database of comparative protein structure models
StringQ09751Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596010glyoxalase I
RefSeq mRNANM_001021918972h- glyoxalase I (glo1), mRNA
European Nucleotide ArchiveCAA20759ENA Protein Mapping
UniParcUPI000012E613UniProt Archive

Literature for glo1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014