glo1 (SPBC12C2.12c)


Gene Standard Nameglo1 Characterisation Statuspublished
Systematic IDSPBC12C2.12c Feature Typeprotein coding
SynonymsSPBC21D10.03c Name Description
Productglyoxalase I Product Size302aa, 34.41 kDa
Genomic Location Chromosome II, 2446543-2447857 (1315nt); CDS:2446698-2447606 (909nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004462lactoylglutathione lyase activityIDAPMID:150422801
heterologous_systemIGISGD:S000004463PMID:15042280
GO:0008270zinc ion bindingIDAPMID:15042280249
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0071470cellular response to osmotic stressTASPMID:1571429535
GO:0006749glutathione metabolic processIDAPMID:1504228013
GO:0019243methylglyoxal catabolic process to D-lactateIMPPMID:150422803
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISOSGD:S000004463GO_REF:00000244205
GO:0005829cytosolIDAPMID:168233722315
GO:0005634nucleusIDAPMID:168233722740
ISOSGD:S000004463GO_REF:0000024
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003407resistance to glyoxalCell growth assayglo1+ (wild type)OverexpressionPECO:0000126PMID:247587164
FYPO:0003406resistance to methylglyoxalCell growth assayglo1+ (wild type)OverexpressionPECO:0000126PMID:247587163
FYPO:0003408sensitive to methylglyoxalCell growth assayglo1ΔNullPECO:0000126PMID:247587161
FYPO:0002060viable vegetative cell populationMicroscopyglo1ΔNullPECO:0000005, PECO:0000137PMID:236978063759
Microscopyglo1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopyglo1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
124465432447857

UTRs

Region Coordinates Reference
three_prime_UTR2447607..2447857PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00903 Pfam IPR004360 Glyoxalase/fosfomycin resistance/dioxygenase domain 167 298 1
PF00903 Pfam IPR004360 Glyoxalase/fosfomycin resistance/dioxygenase domain 11 150 1
PS00935 Prosite Patterns IPR018146 Glyoxalase I, conserved site 93 110 1
PS00934 Prosite Patterns IPR018146 Glyoxalase I, conserved site 169 189 1
PS00935 Prosite Patterns IPR018146 Glyoxalase I, conserved site 241 258 1
PS00934 Prosite Patterns IPR018146 Glyoxalase I, conserved site 14 35 1
PTHR10374:SF0 HMMPANTHER 6 299 1
PTHR10374 HMMPANTHER 6 299 1
3.10.180.10 Gene3D 155 301 2
3.10.180.10 Gene3D 5 154 2
SSF54593 SuperFamily Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase 164 300 2
SSF54593 SuperFamily Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase 6 158 2
TIGR00068 tigrfam IPR004361 Glyoxalase I 7 158 1
TIGR00068 tigrfam IPR004361 Glyoxalase I 160 302 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.94 Da
Charge -2.00
Isoelectric point 6.19
Molecular weight 34.41 kDa
Number of residues 302
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
103572during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
98900during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
103477during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
98493during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
96771during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
11869.61during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
43192.49during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
3.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
monomeric PMID:150422808
Catalytic Activity Attributes
DescriptionQualifierReferenceCount
k(cat)/ K(m)m 4.3x10(7) M(-1) min(-1) PMID:150422801
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3425
conserved in vertebrates3400
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
dad1DASH complex subunit Dad1 Negative GeneticPMID:22681890
SPAC1A6.03cphospholipase (predicted) Positive GeneticPMID:22681890
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
cut2securin, sister chromatid separation inhibitor Negative GeneticPMID:22681890
php3CCAAT-binding factor complex subunit Php3 Negative GeneticPMID:22681890
SPBC1348.10cphospholipase (predicted) Negative GeneticPMID:22681890
vps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
php5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
cph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
mph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
atg6beclin family protein, involved in autophagy Negative GeneticPMID:22681890
pst2Clr6 histone deacetylase complex subunit Pst2 Negative GeneticPMID:22681890
xap5xap-5-like protein Negative GeneticPMID:24957674
hsp3101glyoxylase III Hsp3101 Phenotypic SuppressionPMID:24758716
Dosage Rescue
hsp3102glyoxylase III Hsp3102 Phenotypic SuppressionPMID:24758716
Dosage Rescue
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC12C2.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC12C2.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC12C2.12c BioGRID Interaction Datasets
Expression Viewer SPBC12C2.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC12C2.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC12C2.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC12C2.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC12C2.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC12C2.12c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC12C2.12c Cell Cycle Data
GEO SPBC12C2.12c GEO profiles
PInt SPBC12C2.12c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC12C2.12c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC12C2.12c Fission yeast phenotypic data & analysis
IntEnz4.4.1.5Integrated relational Enzyme database
Rhea4.4.1.5Annotated reactions database
SPD / RIKEN12/12E09Orfeome Localization Data
UniProtKB/SwissProtQ09751Lactoylglutathione lyase
ModBaseQ09751Database of comparative protein structure models
STRINGQ09751Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596010glyoxalase I
RefSeq mRNANM_001021918972h- glyoxalase I (glo1), mRNA
European Nucleotide ArchiveCAA20759.1ENA Protein Mapping
UniParcUPI000012E613UniProt Archive

Literature for glo1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014