glo1 (SPBC12C2.12c)


Gene Standard Nameglo1 Characterisation Statuspublished
Systematic IDSPBC12C2.12c Feature Typeprotein coding
SynonymsSPBC21D10.03c Name Description
Productglyoxalase I Product Size302aa, 34.41 kDa
Genomic Location Chromosome II, 2446543-2447857 (1315nt); CDS:2446698-2447606 (909nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
lactoylglutathione lyase activity1
Annotation ExtensionEvidenceWith/FromReference
zinc ion binding239
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

detoxification


Term NameCount
cellular response to osmotic stress34
Annotation ExtensionEvidenceWith/FromReference
detoxification35
Annotation ExtensionEvidenceWith/FromReference
glutathione metabolic process12
Annotation ExtensionEvidenceWith/FromReference
methylglyoxal catabolic process to D-lactate3
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4209
Annotation ExtensionEvidenceWith/FromReference
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
increased cell population growth rateglo1ΔNull247
resistance to glyoxalglo1+ (wild type)Overexpression4
resistance to methylglyoxalglo1+ (wild type)Overexpression3
sensitive to methylglyoxalglo1ΔNull4
viable vegetative cell populationglo1ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
increased duration of protein phosphorylation during cellular response to methylglyoxal1
affecting sty1glo1ΔNull
increased protein localization to nucleus during cellular response to methylglyoxal1
affecting pap1glo1ΔNull
normal protein localization to cytoplasm7
affecting pap1glo1ΔNull
normal protein phosphorylation during cellular response to heat3
affecting sty1glo1ΔNull
normal protein phosphorylation during cellular response to sorbitol2
affecting sty1glo1ΔNull
viable vegetative cell with normal cell morphologyglo1ΔNull3094
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2446543..2447857
mRNA2446543..2447857
5' UTR2446543..2446697AU006571
CDS2446698..2447606
3' UTR2447607..2447857PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00903 Pfam IPR004360 Glyoxalase/fosfomycin resistance/dioxygenase domain 11 150 1
PF00903 Pfam IPR004360 Glyoxalase/fosfomycin resistance/dioxygenase domain 167 298 1
PS00935 Prosite Patterns IPR018146 Glyoxalase I, conserved site 93 110 1
PS00935 Prosite Patterns IPR018146 Glyoxalase I, conserved site 241 258 1
PS00934 Prosite Patterns IPR018146 Glyoxalase I, conserved site 169 189 1
PS00934 Prosite Patterns IPR018146 Glyoxalase I, conserved site 14 35 1
PTHR10374:SF8 HMMPANTHER 1 194 1
PTHR10374 HMMPANTHER 1 194 1
3.10.180.10 Gene3D IPR029068 Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase 5 154 2
3.10.180.10 Gene3D IPR029068 Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase 155 301 2
SSF54593 SuperFamily IPR029068 Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase 6 158 2
SSF54593 SuperFamily IPR029068 Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase 164 300 2
TIGR00068 tigrfam IPR004361 Glyoxalase I 7 158 1
TIGR00068 tigrfam IPR004361 Glyoxalase I 160 302 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.94 Da
Charge -2.00
Isoelectric point 6.19
Molecular weight 34.41 kDa
Number of residues 302
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
103572during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
98900during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
103477during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
98493during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
96771during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
11869.61during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
43192.49during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
3.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
monomeric PMID:150422809
Catalytic Activity Attributes
DescriptionQualifierReferenceCount
k(cat)/ K(m)m 4.3x10(7) M(-1) min(-1) PMID:150422801
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in bacteria1001
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withatg6autophagy associated beclin family protein Atg6 Negative GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
negative genetic interaction withcph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
negative genetic interaction withcut2securin, sister chromatid separation inhibitor Negative GeneticPMID:22681890
negative genetic interaction withdad1DASH complex subunit Dad1 Negative GeneticPMID:22681890
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
rescued by overexpression ofhsp3101glyoxylase III Hsp3101 Dosage RescuePMID:24758716
rescued byhsp3101glyoxylase III Hsp3101 Phenotypic SuppressionPMID:24758716
rescued by overexpression ofhsp3102glyoxylase III Hsp3102 Dosage RescuePMID:24758716
rescued byhsp3102glyoxylase III Hsp3102 Phenotypic SuppressionPMID:24758716
positive genetic interaction withmph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
negative genetic interaction withphp3CCAAT-binding factor complex subunit Php3 Negative GeneticPMID:22681890
negative genetic interaction withphp5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withpst2Clr6 histone deacetylase complex subunit Pst2 Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
positive genetic interaction withSPAC1A6.03cphospholipase (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPBC1348.10cphospholipase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
negative genetic interaction withvps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
External References
Database Identifier Description
NBRP SPBC12C2.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC12C2.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC12C2.12c BioGRID Interaction Datasets
Expression Viewer SPBC12C2.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC12C2.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC12C2.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC12C2.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC12C2.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC12C2.12c Transcriptome Viewer (Bähler Lab)
GEO SPBC12C2.12c GEO profiles
PInt SPBC12C2.12c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC12C2.12c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC12C2.12c Fission yeast phenotypic data & analysis
Cyclebase SPBC12C2.12c.1 Cell Cycle Data
IntEnz4.4.1.5Integrated relational Enzyme database
Rhea4.4.1.5Annotated reactions database
SPD / RIKEN12/12E09Orfeome Localization Data
UniProtKB/SwissProtQ09751Lactoylglutathione lyase
ModBaseQ09751Database of comparative protein structure models
STRINGQ09751Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596010glyoxalase I
RefSeq mRNANM_001021918972h- glyoxalase I (glo1), mRNA
European Nucleotide ArchiveCAA20759.1ENA Protein Mapping
MetaCycPWY-5386Methylglyoxal degradation I
KEGG_Enzyme00620+4.4.1.5Pyruvate metabolism
UniParcUPI000012E613UniProt Archive
UniPathwayUPA00619Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2

Literature for glo1

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015