pck2 (SPBC12D12.04c)


Gene Standard Namepck2 Characterisation Statuspublished
Systematic IDSPBC12D12.04c Feature Typeprotein coding
Synonymspkc1, sts6 Name Descriptionstaurosporine supersensitive
Productprotein kinase C (PKC)-like Pck2 Product Size1016aa, 116.01 kDa
Genomic Location Chromosome II, 2313301-2309126 (4176nt); CDS:2312837-2309787 (3051nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
metal ion binding743
Annotation ExtensionEvidenceWith/FromReference
protein binding859
Annotation ExtensionEvidenceWith/FromReference
protein kinase C activity2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
fungal-type cell wall biogenesis67
Annotation ExtensionEvidenceWith/FromReference
intracellular signal transduction264
Annotation ExtensionEvidenceWith/FromReference
negative regulation of peptidyl-tyrosine phosphorylation2
Annotation ExtensionEvidenceWith/FromReference
protein phosphorylation132
Annotation ExtensionEvidenceWith/FromReference
regulation of alpha-glucan biosynthetic process3
Annotation ExtensionEvidenceWith/FromReference
regulation of cell wall (1->3)-beta-D-glucan biosynthetic process6
Annotation ExtensionEvidenceWith/FromReference
regulation of establishment or maintenance of cell polarity regulating cell shape26
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site305
Annotation ExtensionEvidenceWith/FromReference
cell septum25
Annotation ExtensionEvidenceWith/FromReference
cell tip194
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased bipolar indexpck2ΔNull27
decreased cell population growth on glucose carbon sourcepck2+ (wild type)Overexpression258
increased monopolar indexpck2ΔNull35
inviable vegetative cell populationpck2+ (wild type)Overexpression1438
normal growth during cellular response to salt stresspck2ΔNull17
normal growth on calcium ionpck2ΔNull7
normal growth on Calcofluor Whitepck2+ (wild type)Knockdown1
pck2D (partial disruption)Not specified
normal growth on cell wall-degrading enzymespck2+ (wild type)Overexpression8
normal growth on cilofunginpck2D (partial disruption)Not specified1
normal growth on glucose carbon sourcepck2+ (wild type)Overexpression207
normal growth on K-252apck2ΔNull3
normal vegetative cell population growth ratepck2+ (wild type)Knockdown70
resistance to cilofunginpck2+ (wild type)Knockdown1
resistance to staurosporinepck2+ (wild type)Overexpression8
sensitive to beta-glucanasepck2ΔNull8
sensitive to papulacandin Bpck2D (partial disruption)Not specified4
sensitive to salt stresspck2ΔNull91
sensitive to staurosporinepck2-8Not specified9
pck2ΔNull
sts6-8Not specified
viable vegetative cell populationpck2ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
abnormal cell polaritypck2+ (wild type)Overexpression7
abnormal cell separation after cytokinesis resulting in chained cellspck2+ (wild type)Overexpression11
abnormal cell wall biogenesispck2ΔNull23
abolished protein binding95
affecting rho1 and pck2362-1017 (1-361)Not specified
affecting rho1 and pck2362-592 (1-361, 593-1017)Not specified
affecting rho1 and pck269-121 (1-68, 122-1017)Not specified
decreased cell wall alpha-glucan levelpck2D (partial disruption)Not specified6
decreased cell wall thickness during vegetative growthpck2D (partial disruption)Not specified3
decreased protein binding66
affecting rho1 and pck21-121 (122-1017)Not specified
affecting rho1 and pck269-238 (1-68, 239-1017)Not specified
decreased transcription from CDRE promoter in response to micafunginpck2ΔNull6
decreased transcription from CDRE promoter in response to salt stresspck2ΔNull6
increased 1,3-beta-D-glucan synthase activitypck2+ (wild type)Overexpression5
increased beta-glucan levelpck2+ (wild type)Overexpression3
increased cell wall alpha-glucan levelpck2+ (wild type)Overexpression5
increased cell wall thickness during vegetative growthpck2+ (wild type)Overexpression9
increased cellular calcium levelpck2+ (wild type)Overexpression8
increased protein tyrosine phosphorylation11
affecting pmk1pck2ΔNull
inviable branched, elongated vegetative cellpck2+ (wild type)Overexpression22
inviable vegetative cellpck2ΔNull1258
irregular cell wall during vegetative growthpck2D (partial disruption)Not specified5
normal 1,3-beta-D-glucan synthase activitypck2D (partial disruption)Not specified2
normal galactomannan levelpck2+ (wild type)Overexpression5
normal protein binding56
affecting rho1 and pck21-149 (150-1017)Not specified
affecting rho1 and pck21-361 (362-1017)Not specified
normal vegetative cell morphologypck2+ (wild type)Knockdown548
vegetative cell lysispck2+ (wild type)Overexpression83
viable pear-shaped vegetative cellpck2ΔNull12
viable spheroid vegetative cellpck2ΔNull50
viable vegetative cell with normal cell morphologypck2ΔNull3094
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in ksg1 serine/threonine protein kinase Ksg1
FYPO affected by mutation in pck2 protein kinase C (PKC)-like Pck2
FYPO affected by mutation in rga2 Rho-type GTPase activating protein Rga2
FYPO affected by mutation in rho1 Rho family GTPase Rho1
FYPO affected by mutation in rho2 Rho family GTPase Rho2
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2313301..2309126
mRNA2313301..2309126
5' UTR2313301..2312838PMID:21511999
CDS2312837..2309787
3' UTR2309786..2309126PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00130 Pfam IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 406 455 3
PF00130 Pfam IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 474 525 3
PF00433 Pfam IPR017892 Protein kinase, C-terminal 963 1006 7
PF02185 Pfam IPR011072 HR1 rho-binding repeat 124 191 3
PF02185 Pfam IPR011072 HR1 rho-binding repeat 8 72 3
PF00069 Pfam IPR000719 Protein kinase domain 684 942 109
SM00239 SMART IPR000008 C2 domain 212 306 12
SM00742 SMART IPR011072 HR1 rho-binding repeat 6 69 3
SM00742 SMART IPR011072 HR1 rho-binding repeat 123 189 3
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 683 942 107
SM00109 SMART IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 406 453 5
SM00109 SMART IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 474 523 5
SM00133 SMART IPR000961 AGC-kinase, C-terminal 943 1005 11
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 689 712 73
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 804 816 97
PS00479 Prosite Patterns IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 406 453 3
PS00479 Prosite Patterns IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 474 523 3
PS50011 Prosite Profiles IPR000719 Protein kinase domain 683 942 110
PS51285 Prosite Profiles IPR000961 AGC-kinase, C-terminal 943 1013 13
PS50081 Prosite Profiles IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 473 523 4
PS50081 Prosite Profiles IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 405 453 4
PTHR24357 HMMPANTHER 76 1016 2
PTHR24357:SF73 HMMPANTHER 76 1016 2
2.60.40.150 Gene3D IPR000008 C2 domain 208 300 19
2.60.40.150 Gene3D IPR000008 C2 domain 351 375 19
3.30.200.20 Gene3D 677 755 111
3.30.60.20 Gene3D 397 466 4
3.30.60.20 Gene3D 467 533 4
1.10.510.10 Gene3D 756 954 112
1.10.287.160 Gene3D IPR011072 HR1 rho-binding repeat 119 184 3
SSF57889 SuperFamily 396 455 5
SSF57889 SuperFamily 466 527 5
SSF46585 SuperFamily IPR011072 HR1 rho-binding repeat 117 191 3
SSF49562 SuperFamily IPR000008 C2 domain 208 299 16
SSF49562 SuperFamily IPR000008 C2 domain 352 375 16
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 680 999 127
Coil ncoils Rabaptin coiled-coil domain 45 66 968
Coil ncoils Rabaptin coiled-coil domain 158 179 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001518C1 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001518
PBO:0001519C2 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001519

Protein Properties

Ave. residue weight 114.18 Da
Charge 26.00
Isoelectric point 8.17
Molecular weight 116.01 kDa
Number of residues 1016
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS72
S20, S27, S86, S649, S988
present during mitotic M phaseS105
present during mitotic M phaseS988
present during mitotic M phaseS649
Annotation ExtensionEvidenceResidueReference
experimental evidence S20 PMID:24763107
experimental evidence S27 PMID:24763107
present during mitotic M phase experimental evidence S72 PMID:21712547
experimental evidence S86 PMID:24763107
present during mitotic M phase experimental evidence S105 PMID:21712547
experimental evidence S649 PMID:24763107
present during mitotic M phase experimental evidence S649 PMID:21712547
experimental evidence S988 PMID:24763107
present during mitotic M phase experimental evidence S988 PMID:21712547
O-phospho-L-threonineT984 698
present during mitotic M phaseT984
Annotation ExtensionEvidenceResidueReference
experimental evidence T984 PMID:24763107
present during mitotic M phase experimental evidence T984 PMID:21712547
phosphorylated residue 1927
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5505during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5561during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5482during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5522during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4300during GO:0072690PECO:0000005,
PECO:0000012
single cellexperimental evidencePMID:16224022
5153during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
557.48during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
774.84during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.8during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withcdc42Rho family GTPase Cdc42 Two-hybridPMID:10504305
forms complex withcpc2RACK1 ortholog Cpc2 Reconstituted ComplexPMID:11263963
affinity captured bycpc2RACK1 ortholog Cpc2 Affinity Capture-WesternPMID:11263963
affinity captured bymkh1MEK kinase (MEKK) Mkh1 Affinity Capture-WesternPMID:17005909
binds activation domain construct withrho1Rho family GTPase Rho1 Two-hybridPMID:10651902
forms complex withrho1Rho family GTPase Rho1 Reconstituted ComplexPMID:10651902
binds activation domain construct withrho1Rho family GTPase Rho1 Two-hybridPMID:10504305
affinity captured byrho2Rho family GTPase Rho2 Affinity Capture-WesternPMID:18793338
binds activation domain construct withrho2Rho family GTPase Rho2 Two-hybridPMID:10504305
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withags1alpha glucan synthase Ags1 Synthetic Growth DefectPMID:10087262
inviable in presence of overexpressedags1alpha glucan synthase Ags1 Dosage LethalityPMID:10087262
overexpression rescuesart1arrestin family protein Art1 Dosage RescuePMID:20739711
rescued by overexpression ofbgs11,3-beta-glucan synthase catalytic subunit Bgs1 Dosage RescuePMID:10504305
enhances phenotype ofcpc2RACK1 ortholog Cpc2 Phenotypic EnhancementPMID:11263963
synthetic growth defect withcpc2RACK1 ortholog Cpc2 Synthetic Growth DefectPMID:11263963
rescued by overexpression ofgls2glucosidase II alpha subunit Gls2 Dosage RescuePMID:10504305
synthetic lethal withits31-phosphatidylinositol-4-phosphate 5-kinase Its3 Synthetic LethalityPMID:15923187
synthetically rescueskin1microtubule affinity-regulating kinase Kin1 Synthetic RescuePMID:23294323
rescueskin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:23294323
rescueskin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:20624220
synthetic growth defect withksg1serine/threonine protein kinase Ksg1 Synthetic Growth DefectPMID:14625898
overexpression rescuesksg1serine/threonine protein kinase Ksg1 Dosage RescuePMID:14625898
synthetically rescued bymkh1MEK kinase (MEKK) Mkh1 Synthetic RescuePMID:17005909
synthetic growth defect withmkh1MEK kinase (MEKK) Mkh1 Synthetic Growth DefectPMID:9199286
rescuesmkh1MEK kinase (MEKK) Mkh1 Phenotypic SuppressionPMID:9199286
rescued by overexpression ofpap1transcription factor Pap1/Caf3 Dosage RescuePMID:1899230
rescued by overexpression ofpck1protein kinase C (PKC)-like Pck1 Dosage RescuePMID:15923187
rescued by overexpression ofpck1protein kinase C (PKC)-like Pck1 Dosage RescuePMID:8943330
synthetic lethal withpck1protein kinase C (PKC)-like Pck1 Synthetic LethalityPMID:10504305
synthetic lethal withpck1protein kinase C (PKC)-like Pck1 Synthetic LethalityPMID:8491190
synthetically rescued bypek1MAP kinase kinase Pek1 Synthetic RescuePMID:17005909
enhances phenotype ofpmk1MAP kinase Pmk1 Phenotypic EnhancementPMID:8943330
synthetically rescued bypmk1MAP kinase Pmk1 Synthetic RescuePMID:17005909
rescued by overexpression ofpmk1MAP kinase Pmk1 Dosage RescuePMID:9199286
rescued bypmk1MAP kinase Pmk1 Phenotypic SuppressionPMID:9199286
rescued by overexpression ofpmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:17881729
synthetically rescuesppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic RescuePMID:9427748
synthetically rescuesppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic RescuePMID:17005909
rescued by overexpression ofptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:17881729
rescued by overexpression ofptc3protein phosphatase 2c homolog 3 Dosage RescuePMID:17881729
rescuesrga4Rho-type GTPase activating protein Rga4 Phenotypic SuppressionPMID:20164182
overexpression rescuesrho1Rho family GTPase Rho1 Dosage RescuePMID:10651902
synthetic growth defect withrho1Rho family GTPase Rho1 Synthetic Growth DefectPMID:24498240
rescued byrho2Rho family GTPase Rho2 Phenotypic SuppressionPMID:24498240
synthetically rescuesrho2Rho family GTPase Rho2 Synthetic RescuePMID:17005909
overexpression rescuesrho2Rho family GTPase Rho2 Dosage RescuePMID:11102532
synthetic growth defect withrho2Rho family GTPase Rho2 Synthetic Growth DefectPMID:11102532
rescued by overexpression ofspk1MAP kinase Spk1 Dosage RescuePMID:1899230
overexpression rescuessts5RNB-like protein Dosage RescuePMID:8886983
rescued bysty1MAP kinase Sty1 Phenotypic SuppressionPMID:9199286
rescued bywis1MAP kinase kinase Wis1 Phenotypic SuppressionPMID:9199286
synthetically rescued byyam8stretch-activated calcium ion channel Yam8 Synthetic RescuePMID:11016847
synthetic lethal withyam8stretch-activated calcium ion channel Yam8 Synthetic LethalityPMID:11016847
overexpression rescuesyam8stretch-activated calcium ion channel Yam8 Dosage RescuePMID:11016847
External References
Database Identifier Description
NBRP SPBC12D12.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC12D12.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC12D12.04c BioGRID Interaction Datasets
Expression Viewer SPBC12D12.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC12D12.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC12D12.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC12D12.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC12D12.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC12D12.04c Transcriptome Viewer (Bähler Lab)
GEO SPBC12D12.04c GEO profiles
PInt SPBC12D12.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC12D12.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC12D12.04c Fission yeast phenotypic data & analysis
Cyclebase SPBC12D12.04c.1 Cell Cycle Data
IntEnz2.7.1.-Integrated relational Enzyme database
Rhea2.7.1.-Annotated reactions database
SPD / RIKEN34/34A04Orfeome Localization Data
UniProtKB/SwissProtP36583Protein kinase C-like 2
ModBaseP36583Database of comparative protein structure models
STRINGP36583Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595950protein kinase C (PKC)-like Pck2
RefSeq mRNANM_001021859972h- protein kinase C (PKC)-like Pck2 (pck2), mRNA
European Nucleotide ArchiveAAA35323.1ENA Protein Mapping
European Nucleotide ArchiveBAA03268.1ENA Protein Mapping
European Nucleotide ArchiveBAA87128.1ENA Protein Mapping
European Nucleotide ArchiveCAA22678.1ENA Protein Mapping
UniParcUPI00001313ECUniProt Archive

Literature for pck2

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015