pck2 (SPBC12D12.04c)


Gene Standard Namepck2 Characterisation Statuspublished
Systematic IDSPBC12D12.04c Feature Typeprotein coding
Synonymspkc1, sts6 Name Descriptionstaurosporine supersensitive
Productprotein kinase C (PKC)-like Pck2 Product Size1016aa, 116.01 kDa
Genomic Location Chromosome II, 2313301-2309126 (4176nt); CDS:2312837-2309787 (3051nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037747
GO:0005515protein bindingIPIrho2PMID:10504305887
IPIrho1PMID:10504305
GO:0004697protein kinase C activityIEA EC:2.7.11.13GO_REF:00000032
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0051519activation of bipolar cell growthIMPPMID:2050195442
GO:0050732negative regulation of peptidyl-tyrosine phosphorylation1
regulates pmk1IMPPMID:9135147
GO:0050850positive regulation of calcium-mediated signalingIMPPMID:169289596
GO:0032949regulation of alpha-glucan biosynthetic processIMPPMID:111025323
GO:0090334regulation of cell wall (1->3)-beta-D-glucan biosynthetic processIMPPMID:105043056
GO:2000769regulation of establishment or maintenance of cell polarity regulating cell shapeIMPPMID:849119025
GO:0032995regulation of fungal-type cell wall biogenesisIMPPMID:849119014
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032153cell division siteIDAPMID:16823372292
GO:0030428cell septumIDAPMID:1065190229
GO:0051286cell tipIDAPMID:16823372186
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaypck2+ (wild type)OverexpressionPECO:0000102, PECO:0000126, PECO:0000005PMID:1101684785
FYPO:0002161normal cell population growth on Calcofluor WhiteCell growth assaypck2+ (wild type)KnockdownPECO:0000102, PECO:0000005PMID:105043052
Cell growth assaypck2D (partial disruption)Not specifiedPECO:0000102, PECO:0000005PMID:10504305
FYPO:0002160normal cell population growth on cilofunginCell growth assaypck2D (partial disruption)Not specifiedPECO:0000102, PECO:0000005PMID:105043051
FYPO:0001164normal cell population growth on glucose carbon sourceCell growth assaypck2+ (wild type)OverexpressionPECO:0000102, PECO:0000150, PECO:0000005, PECO:0000126PMID:11016847101
FYPO:0001420normal vegetative cell population growth rateCell growth assaypck2+ (wild type)KnockdownPMID:1050430529
FYPO:0002162resistance to cilofunginCell growth assaypck2+ (wild type)KnockdownPECO:0000102, PECO:0000005PMID:105043051
FYPO:0000109sensitive to papulacandin BCell growth assaypck2D (partial disruption)Not specifiedPECO:0000102, PECO:0000005PMID:105043054
FYPO:0000113sensitive to staurosporineCell growth assaysts6-8Not specifiedPECO:0000201, PECO:0000005, PECO:0000102PMID:18992306
FYPO:0002060viable vegetative cell populationMicroscopypck2ΔNullPECO:0000005, PECO:0000137PMID:236978063755
Microscopypck2ΔNullPECO:0000004, PECO:0000137PMID:23697806
Microscopypck2ΔNullPMID:20473289
no_namePMID:8491190

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001971abnormal cytokinetic cell separation resulting in chained cellsMicroscopypck2+ (wild type)OverexpressionPMID:105043054
FYPO:0000705abolished protein bindingReporter gene assay69-121 (1-68,122-1017)Not specifiedPMID:1050430530
affecting rho1
affecting rho1Reporter gene assay362-592 (1-361,593-1017)Not specifiedPMID:10504305
affecting rho1Reporter gene assay362-1017 (1-361)Not specifiedPMID:10504305
FYPO:0001081decreased cell wall alpha-glucan levelSubstance quantification evidencepck2D (partial disruption)Not specifiedPMID:105043055
FYPO:0002163decreased cell wall thickness during vegetative growthMicroscopypck2D (partial disruption)Not specifiedPECO:0000126PMID:105043052
FYPO:0001645decreased protein bindingReporter gene assay1-121 (122-1017)Not specifiedPMID:1050430521
affecting rho1
affecting rho1Reporter gene assay69-238 (1-68,239-1017)Not specifiedPMID:10504305
FYPO:0001968increased 1,3-beta-D-glucan synthase activityEnzyme assay datapck2+ (wild type)OverexpressionPMID:105043055
FYPO:0001194increased beta-glucan levelSubstance quantification evidencepck2+ (wild type)OverexpressionPMID:105043053
FYPO:0001084increased cell wall alpha-glucan levelSubstance quantification evidencepck2+ (wild type)OverexpressionPMID:105043055
FYPO:0001035increased cell wall thickness during vegetative growthMicroscopypck2+ (wild type)OverexpressionPECO:0000126PMID:105043055
FYPO:0001198increased cellular calcium levelEnzyme assay datapck2+ (wild type)OverexpressionPECO:0000126, PECO:0000005PMID:110168479
FYPO:0001180increased protein tyrosine phosphorylationWestern blot assaypck2ΔNullPMID:91351473
affecting pmk1
FYPO:0001489inviable vegetative cellMicroscopypck2ΔNullPECO:0000004, PECO:0000137PMID:23697806934
FYPO:0002157normal 1,3-beta-D-glucan synthase activityEnzyme assay datapck2D (partial disruption)Not specifiedPMID:105043052
FYPO:0001079normal galactomannan levelSubstance quantification evidencepck2+ (wild type)OverexpressionPMID:105043055
FYPO:0000703normal protein bindingReporter gene assay1-361 (362-1017)Not specifiedPMID:1050430518
affecting rho1
affecting rho1Reporter gene assay1-149 (150-1017)Not specifiedPMID:10504305
FYPO:0001315normal vegetative cell morphologyMicroscopypck2+ (wild type)KnockdownPMID:1050430542
FYPO:0000647vegetative cell lysisMicroscopypck2+ (wild type)OverexpressionPECO:0000126, PECO:0000005PMID:1101684736
FYPO:0002110viable bottle-shaped vegetative cellMicroscopypck2D (partial disruption)Not specifiedPECO:0000126, PECO:0000005PMID:105043056
FYPO:0002380viable spheroid vegetative cellMicroscopypck2ΔNullPECO:0000005, PECO:0000137PMID:2369780633
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopypck2ΔNullPECO:0000005, PECO:0000137PMID:236978063078
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
123133012309126

UTRs

Region Start End Reference
five_prime_UTR23133012312838PMID:21511999
three_prime_UTR23097862309126PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 684 942 109
PF02185 Pfam IPR011072 HR1 rho-binding repeat 8 72 3
PF02185 Pfam IPR011072 HR1 rho-binding repeat 124 191 3
PF00130 Pfam IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 474 525 3
PF00130 Pfam IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 406 455 3
PF00433 Pfam IPR017892 Protein kinase, C-terminal 963 1006 7
SM00742 SMART IPR011072 HR1 rho-binding repeat 123 189 3
SM00742 SMART IPR011072 HR1 rho-binding repeat 6 69 3
SM00109 SMART IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 474 523 5
SM00109 SMART IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 406 453 5
SM00220 SMART IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain 683 942 73
SM00133 SMART IPR000961 AGC-kinase, C-terminal 943 1005 13
SM00239 SMART IPR000008 C2 domain 212 306 15
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 689 712 73
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 804 816 97
PS00479 Prosite Patterns IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 406 453 3
PS00479 Prosite Patterns IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 474 523 3
PS51285 Prosite Profiles IPR000961 AGC-kinase, C-terminal 943 1013 13
PS50011 Prosite Profiles IPR000719 Protein kinase domain 683 942 110
PS50081 Prosite Profiles IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 473 523 4
PS50081 Prosite Profiles IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 405 453 4
PTHR24357:SF0 HMMPANTHER 104 1015 2
PTHR24357 HMMPANTHER 104 1015 2
G3DSA:3.30.200.20 Gene3D 677 755 109
G3DSA:2.60.40.150 Gene3D 208 300 19
G3DSA:2.60.40.150 Gene3D 351 375 19
G3DSA:3.30.60.20 Gene3D 397 466 4
G3DSA:3.30.60.20 Gene3D 467 533 4
G3DSA:1.10.287.160 Gene3D IPR011072 119 184 3
G3DSA:1.10.510.10 Gene3D 756 954 112
SSF49562 SuperFamily IPR000008 208 299 16
SSF49562 SuperFamily IPR000008 352 375 16
SSF56112 SuperFamily IPR011009 680 999 127
SSF57889 SuperFamily 396 455 5
SSF57889 SuperFamily 466 527 5
SSF46585 SuperFamily IPR011072 117 191 3
Coil ncoils Rabaptin coiled-coil domain 45 66 975
Coil ncoils Rabaptin coiled-coil domain 158 179 975
Low complexity (SEG) seg 55 66
Low complexity (SEG) seg 86 113
Low complexity (SEG) seg 455 463
Low complexity (SEG) seg 546 571
Low complexity (SEG) seg 579 590

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001518C1 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001518
PBO:0001519C2 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001519

Protein Properties

Ave. residue weight 114.18 Da
Charge 26.00
Isoelectric point 8.17
Molecular weight 116.01 kDa
Number of residues 1016
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residuePMID:195477441192
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
557.48during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
4300during GO:0072690PECO:0000005,
PECO:0000012
single_cellexperimental evidencePMID:16224022
774.84during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
2.8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
wis1MAP kinase kinase Wis1 Phenotypic SuppressionPMID:9199286
rga4Rho-type GTPase activating protein Rga4 Phenotypic SuppressionPMID:20164182
pmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:17881729
kin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:23294323
Synthetic Rescue
Phenotypic SuppressionPMID:20624220
art1arrestin family protein Art1 Dosage RescuePMID:20739711
gls2glucosidase II Gls2 Dosage RescuePMID:10504305
rho1Rho family GTPase Rho1 Dosage RescuePMID:10651902
Phenotypic EnhancementPMID:10504305
pek1MAP kinase kinase Pek1 Synthetic RescuePMID:17005909
rho2Rho family GTPase Rho2 Synthetic RescuePMID:17005909
Dosage RescuePMID:11102532
Synthetic Growth Defect
sty1MAP kinase Sty1 Phenotypic SuppressionPMID:9199286
pck1protein kinase C (PKC)-like Pck1 Dosage RescuePMID:15923187
Synthetic LethalityPMID:10504305
Synthetic LethalityPMID:8491190
pmk1MAP kinase Pmk1 Phenotypic EnhancementPMID:8943330
Synthetic RescuePMID:17005909
Phenotypic SuppressionPMID:9199286
Dosage Rescue
ptc3protein phosphatase 2c homolog 3 Dosage RescuePMID:17881729
pap1transcription factor Pap1/Caf3 Dosage RescuePMID:1899230
ptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:17881729
yam8calcium channel regulatory subunit Yam8 Synthetic RescuePMID:11016847
Dosage Rescue
Synthetic Lethality
cpc2RACK1 ortholog Cpc2 Phenotypic EnhancementPMID:11263963
Synthetic Growth Defect
its31-phosphatidylinositol-4-phosphate 5-kinase Its3 Synthetic LethalityPMID:15923187
ags1alpha-1,4-glucan synthase Ags1 Synthetic Growth DefectPMID:10087262
Dosage Lethality
sts5RNB-like protein Dosage RescuePMID:8886983
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Dosage RescuePMID:10504305
spk1MAP kinase Spk1 Dosage RescuePMID:1899230
ppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic RescuePMID:9427748
Synthetic RescuePMID:17005909
ksg1serine/threonine protein kinase Ksg1 Synthetic Growth DefectPMID:14625898
Dosage Rescue
mkh1MEK kinase (MEKK) Mkh1 Synthetic RescuePMID:17005909
Synthetic Growth DefectPMID:9199286
Phenotypic Suppression
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rho2Rho family GTPase Rho2 Affinity Capture-WesternPMID:18793338
Two-hybridPMID:10504305
cpc2RACK1 ortholog Cpc2 Affinity Capture-WesternPMID:11263963
Reconstituted Complex
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
mkh1MEK kinase (MEKK) Mkh1 Affinity Capture-WesternPMID:17005909
cdc42Rho family GTPase Cdc42 Two-hybridPMID:10504305
rho1Rho family GTPase Rho1 Two-hybridPMID:10651902
Reconstituted Complex
Two-hybridPMID:10504305
External References
Database Identifier Description
NBRP SPBC12D12.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC12D12.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC12D12.04c BioGRID Interaction Datasets
Expression Viewer SPBC12D12.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC12D12.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC12D12.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC12D12.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC12D12.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC12D12.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC12D12.04c Cell Cycle Data
GEO SPBC12D12.04c GEO profiles
PInt SPBC12D12.04c Protein-Protein Interaction Predictor (Bähler Lab)
IntEnz2.7.1.-Integrated relational Enzyme database
Rhea2.7.1.-Annotated reactions database
EntrezGene2539649protein kinase C (PKC)-like Pck2
WikiGene2539649protein kinase C (PKC)-like Pck2
SPD / RIKEN34/34A04Orfeome Localization Data
UniProtKB/SwissProtP36583Protein kinase C-like 2
ModBaseP36583Database of comparative protein structure models
StringP36583Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595950protein kinase C (PKC)-like Pck2
RefSeq mRNANM_001021859972h- protein kinase C (PKC)-like Pck2 (pck2), mRNA
European Nucleotide ArchiveAAA35323ENA Protein Mapping
European Nucleotide ArchiveBAA03268ENA Protein Mapping
European Nucleotide ArchiveBAA87128ENA Protein Mapping
European Nucleotide ArchiveCAA22678ENA Protein Mapping
UniParcUPI00001313ECUniProt Archive

Literature for pck2

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014