pck2 (SPBC12D12.04c)


Gene Standard Namepck2 Characterisation Statuspublished
Systematic IDSPBC12D12.04c Feature Typeprotein coding
Synonymspkc1, sts6 Name DescriptionSTaurosporine Supersensitive
Productprotein kinase C (PKC)-like Pck2 Product Size1016aa, 116.01 kDa
Genomic Location Chromosome II, 2313301-2309126 (4176nt); CDS:2312837-2309787 (3051nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
metal ion binding743
Annotation ExtensionEvidenceWith/FromReference
protein binding863
Annotation ExtensionEvidenceWith/FromReference
protein kinase C activity2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
fungal-type cell wall biogenesis64
Annotation ExtensionEvidenceWith/FromReference
intracellular signal transduction217
Annotation ExtensionEvidenceWith/FromReference
negative regulation of peptidyl-tyrosine phosphorylation2
Annotation ExtensionEvidenceWith/FromReference
protein phosphorylation170
Annotation ExtensionEvidenceWith/FromReference
regulation of alpha-glucan biosynthetic process3
Annotation ExtensionEvidenceWith/FromReference
regulation of cell wall (1->3)-beta-D-glucan biosynthetic process6
Annotation ExtensionEvidenceWith/FromReference
regulation of establishment or maintenance of cell polarity regulating cell shape26
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
barrier septum22
Annotation ExtensionEvidenceWith/FromReference
cell division site309
Annotation ExtensionEvidenceWith/FromReference
cell tip200
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
decreased bipolar indexpck2Δ27
decreased cell population growth on glucose carbon sourcepck2+286
increased monopolar indexpck2Δ35
inviable vegetative cell populationpck2+1444
normal growth during cellular response to salt stresspck2Δ19
normal growth on calcium ionpck2Δ8
normal growth on Calcofluor Whitepck2D (partial disruption)5
pck2+
normal growth on cell wall-degrading enzymespck2+8
normal growth on cilofunginpck2D (partial disruption)1
normal growth on glucose carbon sourcepck2+240
normal growth on K-252apck2Δ3
normal vegetative cell population growth ratepck2+77
resistance to cilofunginpck2+1
resistance to staurosporinepck2+8
sensitive to beta-glucanasepck2Δ8
sensitive to papulacandin Bpck2D (partial disruption)4
sensitive to salt stresspck2Δ94
sensitive to staurosporinepck2Δ10
sts6-8
pck2-8
viable vegetative cell populationpck2Δ3819

Cell Phenotype

Term NameGenotypesCount
abnormal cell polaritypck2+7
abnormal cell separation after cytokinesis resulting in chained cellspck2+11
abnormal cell wall biogenesispck2Δ24
abolished protein binding104
affecting rho1 and pck2362-1017 (1-361)
affecting rho1 and pck2362-592 (1-361, 593-1017)
affecting rho1 and pck269-121 (1-68, 122-1017)
decreased cell wall alpha-glucan levelpck2D (partial disruption)6
decreased cell wall thickness during vegetative growthpck2D (partial disruption)3
decreased protein binding78
affecting rho1 and pck269-238 (1-68, 239-1017)
affecting rho1 and pck21-121 (122-1017)
decreased transcription from CDRE promoter in response to micafunginpck2Δ6
decreased transcription from CDRE promoter in response to salt stresspck2Δ6
increased 1,3-beta-D-glucan synthase activitypck2+5
increased beta-glucan levelpck2+7
increased cell wall alpha-glucan levelpck2+7
increased cell wall thickness during vegetative growthpck2+11
increased cellular calcium levelpck2+8
increased protein tyrosine phosphorylation11
affecting pmk1pck2Δ
inviable branched, elongated vegetative cellpck2+23
inviable vegetative cellpck2Δ1337
irregular cell wall during vegetative growthpck2D (partial disruption)5
normal 1,3-beta-D-glucan synthase activitypck2D (partial disruption)3
normal galactomannan levelpck2+6
normal protein binding60
affecting rho1 and pck21-361 (362-1017)
affecting rho1 and pck21-149 (150-1017)
normal vegetative cell morphologypck2+550
vegetative cell lysispck2+86
viable pear-shaped vegetative cellpck2Δ12
viable spheroid vegetative cellpck2Δ50
viable vegetative cell with normal cell morphologypck2Δ3097

Multi-allele phenotypes


Population Phenotype

Term NameGenotypes
normal growth during cellular response to salt stresspck2Δ, kin1Δ

Cell Phenotype

Term NameGenotypes
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in ksg1 serine/threonine protein kinase Ksg1
FYPO affected by mutation in pck2 protein kinase C (PKC)-like Pck2
FYPO affected by mutation in rga2 Rho-type GTPase activating protein Rga2
FYPO affected by mutation in rho1 Rho family GTPase Rho1
FYPO affected by mutation in rho2 Rho family GTPase Rho2
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2313301..2309126
mRNA2313301..2309126
5' UTR2313301..2312838PMID:21511999
CDS2312837..2309787
3' UTR2309786..2309126PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00130 Pfam IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 406 455 3
PF00130 Pfam IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 474 525 3
PF00433 Pfam IPR017892 Protein kinase, C-terminal 963 1004 8
PF02185 Pfam IPR011072 HR1 rho-binding repeat 125 188 3
PF02185 Pfam IPR011072 HR1 rho-binding repeat 8 66 3
PF00069 Pfam IPR000719 Protein kinase domain 684 942 108
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 683 942 107
SM00239 SMART IPR000008 C2 domain 212 306 12
SM00133 SMART IPR000961 AGC-kinase, C-terminal 943 1005 11
SM00109 SMART IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 406 453 5
SM00109 SMART IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 474 523 5
SM00742 SMART IPR011072 HR1 rho-binding repeat 6 69 3
SM00742 SMART IPR011072 HR1 rho-binding repeat 123 189 3
PS50011 Prosite Profiles IPR000719 Protein kinase domain 683 942 110
PS50081 Prosite Profiles IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 473 523 4
PS50081 Prosite Profiles IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 405 453 4
PS51285 Prosite Profiles IPR000961 AGC-kinase, C-terminal 943 1013 13
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 689 712 73
PS00479 Prosite Patterns IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 474 523 3
PS00479 Prosite Patterns IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 406 453 3
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 804 816 97
PTHR24356 HMMPANTHER 388 549 13
PTHR24356 HMMPANTHER 5 45 13
PTHR24356 HMMPANTHER 634 1003 13
PTHR24356:SF203 HMMPANTHER 388 549 2
PTHR24356:SF203 HMMPANTHER 5 45 2
PTHR24356:SF203 HMMPANTHER 634 1003 2
1.10.287.160 Gene3D IPR011072 HR1 rho-binding repeat 119 184 3
3.30.200.20 Gene3D GPCR kinase 677 755 111
1.10.510.10 Gene3D GPCR kinase 756 954 112
2.60.40.150 Gene3D IPR000008 C2 domain 351 375 19
2.60.40.150 Gene3D IPR000008 C2 domain 208 300 19
3.30.60.20 Gene3D Protein kinase C, theta 397 466 4
3.30.60.20 Gene3D Protein kinase C, theta 467 533 4
SSF49562 SuperFamily IPR000008 C2 domain 208 299 16
SSF49562 SuperFamily IPR000008 C2 domain 352 375 16
SSF57889 SuperFamily 396 455 5
SSF57889 SuperFamily 466 527 5
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 680 999 127
SSF46585 SuperFamily IPR011072 HR1 rho-binding repeat 117 191 3
Coil ncoils Predicted coiled-coil protein (DUF2205) 45 65 1048
Coil ncoils Predicted coiled-coil protein (DUF2205) 158 178 1048

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001518C1 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001518
PBO:0001519C2 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001519

Protein Properties

Ave. residue weight 114.18 Da
Charge 26.00
Isoelectric point 8.17
Molecular weight 116.01 kDa
Number of residues 1016
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1674
present during mitotic M phaseS988
present during mitotic M phaseS72
S20, S27, S86, S649, S988
present during mitotic M phaseS105
present during mitotic M phaseS649
Annotation ExtensionEvidenceResidueReference
experimental evidence S20 PMID:24763107
experimental evidence S27 PMID:24763107
present during mitotic M phase experimental evidence S72 PMID:21712547
experimental evidence S86 PMID:24763107
present during mitotic M phase experimental evidence S105 PMID:21712547
experimental evidence S649 PMID:24763107
present during mitotic M phase experimental evidence S649 PMID:21712547
experimental evidence S988 PMID:24763107
present during mitotic M phase experimental evidence S988 PMID:21712547
O-phospho-L-threonineT984 699
present during mitotic M phaseT984
Annotation ExtensionEvidenceResidueReference
experimental evidence T984 PMID:24763107
present during mitotic M phase experimental evidence T984 PMID:21712547
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5505during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5561during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5482during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5522during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
557.48during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
5153during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4300during GO:0072690PECO:0000012,
PECO:0000005
single cellexperimental evidencePMID:16224022
774.84during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.8during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3421
conserved in vertebrates3396
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPBC12D12.04c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withcdc42Rho family GTPase Cdc42 Two-hybridPMID:10504305
binds activation domain construct withrho2Rho family GTPase Rho2 Two-hybridPMID:10504305
binds activation domain construct withrho1Rho family GTPase Rho1 Two-hybridPMID:10504305
forms complex withrho1Rho family GTPase Rho1 Reconstituted ComplexPMID:10651902
forms complex withcpc2RACK1 ortholog Cpc2 Reconstituted ComplexPMID:11263963
affinity captured bymkh1MEK kinase (MEKK) Mkh1 Affinity Capture-WesternPMID:17005909
affinity captured byrho2Rho family GTPase Rho2 Affinity Capture-WesternPMID:18793338
affinity captured bycpc2RACK1 ortholog Cpc2 Affinity Capture-WesternPMID:11263963
Genetic Interactions

Source: BioGRID

Load gene that interact geneitically with SPBC12D12.04c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescued by overexpression ofpck1protein kinase C (PKC)-like Pck1 Dosage RescuePMID:8943330
rescued by overexpression ofspk1MAP kinase Spk1 Dosage RescuePMID:1899230
rescued by overexpression ofpap1transcription factor Pap1/Caf3 Dosage RescuePMID:1899230
rescued by overexpression ofptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:17881729
rescued by overexpression ofpmk1MAP kinase Pmk1 Dosage RescuePMID:9199286
rescued by overexpression ofbgs11,3-beta-glucan synthase catalytic subunit Bgs1 Dosage RescuePMID:10504305
rescued by overexpression ofgls2glucosidase II alpha subunit Gls2 Dosage RescuePMID:10504305
rescued by overexpression ofptc3protein phosphatase 2c homolog 3 Dosage RescuePMID:17881729
rescued by overexpression ofpmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:17881729
synthetically rescuesppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic RescuePMID:17005909
synthetically rescueskin1microtubule affinity-regulating kinase Kin1 Synthetic RescuePMID:23294323
synthetically rescuesrho2Rho family GTPase Rho2 Synthetic RescuePMID:17005909
synthetically rescued bypmk1MAP kinase Pmk1 Synthetic RescuePMID:17005909
synthetically rescued byyam8stretch-activated calcium ion channel Yam8 Synthetic RescuePMID:11016847
synthetically rescued bymkh1MEK kinase (MEKK) Mkh1 Synthetic RescuePMID:17005909
synthetically rescued bypek1MAP kinase kinase Pek1 Synthetic RescuePMID:17005909
rescuesmkh1MEK kinase (MEKK) Mkh1 Phenotypic SuppressionPMID:9199286
rescueskin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:20624220
rescuesrga4Rho-type GTPase activating protein Rga4 Phenotypic SuppressionPMID:20164182
rescued bysty1MAP kinase Sty1 Phenotypic SuppressionPMID:9199286
rescued bypmk1MAP kinase Pmk1 Phenotypic SuppressionPMID:9199286
rescued byrho2Rho family GTPase Rho2 Phenotypic SuppressionPMID:24498240
rescued bywis1MAP kinase kinase Wis1 Phenotypic SuppressionPMID:9199286
enhances phenotype ofpmk1MAP kinase Pmk1 Phenotypic EnhancementPMID:8943330
enhances phenotype ofcpc2RACK1 ortholog Cpc2 Phenotypic EnhancementPMID:11263963
synthetic growth defect withksg1serine/threonine protein kinase Ksg1 Synthetic Growth DefectPMID:14625898
synthetic growth defect withrho1Rho family GTPase Rho1 Synthetic Growth DefectPMID:24498240
synthetic growth defect withcpc2RACK1 ortholog Cpc2 Synthetic Growth DefectPMID:11263963
synthetic growth defect withags1alpha glucan synthase Ags1 Synthetic Growth DefectPMID:10087262
synthetic growth defect withrho2Rho family GTPase Rho2 Synthetic Growth DefectPMID:11102532
synthetic growth defect withmkh1MEK kinase (MEKK) Mkh1 Synthetic Growth DefectPMID:9199286
inviable in presence of overexpressedags1alpha glucan synthase Ags1 Dosage LethalityPMID:10087262
overexpression rescuessts5RNB-like protein Dosage RescuePMID:8886983
overexpression rescuesrho1Rho family GTPase Rho1 Dosage RescuePMID:10651902
overexpression rescuesksg1serine/threonine protein kinase Ksg1 Dosage RescuePMID:14625898
overexpression rescuesart1arrestin family protein Art1 Dosage RescuePMID:20739711
overexpression rescuesrho2Rho family GTPase Rho2 Dosage RescuePMID:11102532
overexpression rescuesyam8stretch-activated calcium ion channel Yam8 Dosage RescuePMID:11016847
synthetic lethal withyam8stretch-activated calcium ion channel Yam8 Synthetic LethalityPMID:11016847
synthetic lethal withits31-phosphatidylinositol-4-phosphate 5-kinase Its3 Synthetic LethalityPMID:15923187
synthetic lethal withpck1protein kinase C (PKC)-like Pck1 Synthetic LethalityPMID:8491190
External References
Database Identifier Description
NBRP SPBC12D12.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC12D12.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC12D12.04c BioGRID Interaction Datasets
Expression Viewer SPBC12D12.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC12D12.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC12D12.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC12D12.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC12D12.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC12D12.04c Transcriptome Viewer (Bähler Lab)
GEO SPBC12D12.04c GEO profiles
PInt SPBC12D12.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC12D12.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC12D12.04c Fission yeast phenotypic data & analysis
Cyclebase SPBC12D12.04c.1 Cell Cycle Data
IntEnz2.7.1.-Integrated relational Enzyme database
Rhea2.7.1.-Annotated reactions database
SPD / RIKEN34/34A04Orfeome Localization Data
UniProtKB/SwissProtP36583Protein kinase C-like 2
ModBaseP36583Database of comparative protein structure models
STRINGP36583Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595950protein kinase C (PKC)-like Pck2
RefSeq mRNANM_001021859972h- protein kinase C (PKC)-like Pck2 (pck2), mRNA
European Nucleotide ArchiveAB027824ENA EMBL mapping
European Nucleotide ArchiveCU329671ENA EMBL mapping
European Nucleotide ArchiveD14338ENA EMBL mapping
European Nucleotide ArchiveL07637ENA EMBL mapping
European Nucleotide ArchiveAAA35323ENA Protein Mapping
European Nucleotide ArchiveAAA35323.1ENA Protein Mapping
European Nucleotide ArchiveBAA03268ENA Protein Mapping
European Nucleotide ArchiveBAA03268.1ENA Protein Mapping
European Nucleotide ArchiveBAA87128ENA Protein Mapping
European Nucleotide ArchiveBAA87128.1ENA Protein Mapping
European Nucleotide ArchiveCAA22678ENA Protein Mapping
European Nucleotide ArchiveCAA22678.1ENA Protein Mapping
UniParcUPI00001313ECUniProt Archive

Literature for pck2

Search: Europe PMC or PubMed

Release Version: PomBase:29_56 - 12 Nov 2015