pck2 (SPBC12D12.04c)

Gene Standard Namepck2 ChromosomeII
Systematic IDSPBC12D12.04c Gene Start2313301
Synonymspkc1, sts6 Gene End2309126
Productprotein kinase C (PKC)-like Pck2 Gene Length4176
Feature Typeprotein coding CDS Start2312837
Name Descriptionstaurosporine supersensitive CDS End2309787
Characterisation Statuspublished CDS Length3051

Ensembl Gene Location
  1. sad1
  2. cdm1
  3. cct1
  4. pck2
  5. rev7
  6. SPBC12D12.05c
  7. srb11
  8. trx2
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000113sensitive to staurosporineCell growth assaysts6-8 (unknown)unknownNot specifiedPECO:0000005, PECO:0000201, PECO:0000102PMID:1899230
FYPO:0000647cell lysisMicroscopypck2+ (wild type)wild_typeOverexpressionPECO:0000005, PECO:0000126PMID:11016847
FYPO:0001161decreased aerobic cell population growth on glucose carbon sourceCell growth assaypck2+ (wild type)wild_typeOverexpressionPECO:0000126, PECO:0000102, PECO:0000005PMID:11016847
FYPO:0001164normal cell population growth on glucose carbon sourceCell growth assaypck2+ (wild type)wild_typeOverexpressionPECO:0000150, PECO:0000102, PECO:0000005, PECO:0000126PMID:11016847
FYPO:0001180increased protein tyrosine phosphorylationWestern blot assaypck2delta (deletion)deletionPMID:9135147
assayed_using pmk1
FYPO:0001198increased cellular calcium levelEnzyme assay datapck2+ (wild type)wild_typeOverexpressionPECO:0000005, PECO:0000126PMID:11016847
FYPO:0002060viable vegetative cell populationno_name (unrecorded)unrecordedPMID:8491190
Microscopypck2delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0004697protein kinase C activityIEAEC:2.7.11.13GO_REF:0000003
GO:0005515protein bindingIPIrho1PMID:10504305
IPIrho2PMID:10504305
GO:0005524ATP bindingIEAUniProtKB:KW-0067GO_REF:0000037
GO:0046872metal ion bindingIEAUniProtKB:KW-0479GO_REF:0000037
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0006468protein phosphorylationICGO:0004697GO_REF:0000001
GO:0032949regulation of alpha-glucan biosynthetic processIMPPMID:11102532
GO:0032995regulation of fungal-type cell wall biogenesisIMPPMID:8491190
GO:0035556intracellular signal transductionICGO:0004697GO_REF:0000001
GO:0050732negative regulation of peptidyl-tyrosine phosphorylation
has_regulation_target pmk1IMPPMID:9135147
GO:0050850positive regulation of calcium-mediated signalingIMPPMID:16928959
GO:0051519activation of bipolar cell growthIMPPMID:20501954
GO:0090334regulation of cell wall (1->3)-beta-D-glucan biosynthetic processIMPPMID:10504305
GO:2000769regulation of establishment or maintenance of cell polarity regulating cell shapeIMPPMID:8491190
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0030428cell septumIDAPMID:10651902
GO:0032153cell division siteIDAPMID:16823372
GO:0051286cell tipIDAPMID:16823372
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
123133012309126

References

Region Start End Reference
three_prime_UTR23097862309126PMID:21511999
five_prime_UTR23133012312838PMID:21511999
three_prime_UTR23097862309126PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00130 IPR002219 Pfam Protein kinase C-like, phorbol ester/diacylglycerol binding 474 525 3
PF00130 IPR002219 Pfam Protein kinase C-like, phorbol ester/diacylglycerol binding 406 455 3
PF00433 IPR017892 Pfam Protein kinase, C-terminal 963 1006 9
PF00069 IPR000719 Pfam Protein kinase, catalytic domain 684 942 109
PF02185 IPR011072 Pfam HR1 rho-binding repeat 125 191 3
PF02185 IPR011072 Pfam HR1 rho-binding repeat 7 72 3
SM00742 IPR011072 SMART HR1 rho-binding repeat 123 189 3
SM00742 IPR011072 SMART HR1 rho-binding repeat 6 69 3
SM00109 IPR002219 SMART Protein kinase C-like, phorbol ester/diacylglycerol binding 474 523 5
SM00109 IPR002219 SMART Protein kinase C-like, phorbol ester/diacylglycerol binding 406 453 5
SM00220 IPR002290 SMART Serine/threonine- / dual specificity protein kinase, catalytic domain 683 942 74
SM00133 IPR000961 SMART AGC-kinase, C-terminal 943 1005 13
SM00239 IPR000008 SMART C2 calcium-dependent membrane targeting 212 306 15
PS00107 IPR017441 Prosite Patterns Protein kinase, ATP binding site 689 712 73
PS00108 IPR008271 Prosite Patterns Serine/threonine-protein kinase, active site 804 816 97
PS00479 IPR002219 Prosite Patterns Protein kinase C-like, phorbol ester/diacylglycerol binding 406 453 3
PS00479 IPR002219 Prosite Patterns Protein kinase C-like, phorbol ester/diacylglycerol binding 474 523 3
PS51285 IPR000961 Prosite Profiles AGC-kinase, C-terminal 943 1013 13
PS50011 IPR000719 Prosite Profiles Protein kinase, catalytic domain 683 942 110
PS50081 IPR002219 Prosite Profiles Protein kinase C-like, phorbol ester/diacylglycerol binding 473 523 4
PS50081 IPR002219 Prosite Profiles Protein kinase C-like, phorbol ester/diacylglycerol binding 405 453 4
G3DSA:3.30.200.20 Gene3D 677 755 108
G3DSA:2.60.40.150 Gene3D 208 300 19
G3DSA:2.60.40.150 Gene3D 351 375 19
G3DSA:3.30.60.20 Gene3D 397 466 4
G3DSA:3.30.60.20 Gene3D 467 533 4
G3DSA:1.10.287.160 IPR011072 Gene3D 119 184 3
G3DSA:1.10.510.10 Gene3D 756 954 111
SSF49562 IPR008973 SuperFamily 208 299 16
SSF49562 IPR008973 SuperFamily 352 375 16
SSF56112 IPR011009 SuperFamily 680 999 127
SSF57889 SuperFamily 396 455 5
SSF57889 SuperFamily 466 527 5
SSF46585 IPR011072 SuperFamily 117 191 3
PTHR24357:SF0 hmmpanther 4 1012 2
PTHR24357 hmmpanther 4 1012 2
Coil ncoils Rabaptin coiled-coil domain 45 66 974
Coil ncoils Rabaptin coiled-coil domain 158 179 974

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residuePMID:19547744

Manually Curated Family or Sub-families

Term IDTerm NameReferenceAll Genes
PBO:0001518C1 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001518
PBO:0001519C2 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001519

Protein Properties

Ave. residue weight 114.18 Da
Charge 26.00
Isoelectric point 8.17
Molecular weight 116.01 kDa
Number of residues 1016
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide774.84PMID:23101633
experimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide557.48PMID:23101633
experimental evidencesingle-celled organism vegetative growth phasePECO:0000201,
PECO:0000005		single_cell4300PMID:16224022
RNA levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide1.1PMID:23101633
experimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide2.8PMID:23101633
Species Distribution
DescriptionQualifierReference
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ptc3protein phosphatase 2c homolog 3 Dosage RescuePMID:17881729
ptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:17881729
rho2Rho family GTPase Rho2 Synthetic RescuePMID:17005909
Synthetic Growth DefectPMID:11102532
Dosage Rescue
pap1transcription factor Pap1/Caf3 Dosage RescuePMID:1899230
spk1MAP kinase Spk1 Dosage RescuePMID:1899230
cpc2RACK1 ortholog Cpc2 Phenotypic EnhancementPMID:11263963
Synthetic Growth Defect
pmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:17881729
yam8calcium channel regulatory subunit Yam8 Synthetic RescuePMID:11016847
Synthetic Lethality
Dosage Rescue
sty1MAP kinase Sty1 Phenotypic SuppressionPMID:9199286
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Dosage RescuePMID:10504305
rho1Rho family GTPase Rho1 Dosage RescuePMID:10651902
rga4Rho-type GTPase activating protein Rga4 Phenotypic SuppressionPMID:20164182
pek1MAP kinase kinase Pek1 Synthetic RescuePMID:17005909
ppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic RescuePMID:9427748
Synthetic RescuePMID:17005909
wis1MAP kinase kinase Wis1 Phenotypic SuppressionPMID:9199286
gls2glucosidase II Gls2 Dosage RescuePMID:10504305
pck1protein kinase C (PKC)-like Pck1 Synthetic LethalityPMID:10504305
Synthetic LethalityPMID:8491190
Dosage RescuePMID:15923187
sts5RNB-like protein Dosage RescuePMID:8886983
pmk1MAP kinase Pmk1 Synthetic RescuePMID:17005909
Phenotypic SuppressionPMID:9199286
Dosage Rescue
Phenotypic EnhancementPMID:8943330
mkh1MEK kinase (MEKK) Mkh1 Synthetic RescuePMID:17005909
Phenotypic SuppressionPMID:9199286
Synthetic Growth Defect
ags1alpha-1,4-glucan synthase Ags1 Synthetic Growth DefectPMID:10087262
Dosage Lethality
art1arrestin family protein Dosage RescuePMID:20739711
ksg1serine/threonine protein kinase Ksg1 Synthetic Growth DefectPMID:14625898
Dosage Rescue
kin1microtubule affinity-regulating kinase Kin1 Synthetic RescuePMID:23294323
Phenotypic Suppression
Phenotypic SuppressionPMID:20624220
its31-phosphatidylinositol-4-phosphate 5-kinase Its3 Synthetic LethalityPMID:15923187
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rho1Rho family GTPase Rho1 Two-hybridPMID:10504305
Reconstituted ComplexPMID:10651902
Two-hybrid
cdc42Rho family GTPase Cdc42 Two-hybridPMID:10504305
mkh1MEK kinase (MEKK) Mkh1 Affinity Capture-WesternPMID:17005909
ags1alpha-1,4-glucan synthase Ags1 Co-localizationPMID:10087262
cpc2RACK1 ortholog Cpc2 Affinity Capture-WesternPMID:11263963
Reconstituted Complex
rho2Rho family GTPase Rho2 Two-hybridPMID:10504305
Affinity Capture-WesternPMID:18793338
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External References
Database Identifier Description
NBRP SPBC12D12.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC12D12.04c Retrieval of eukaryotic orthologs
BioGrid SPBC12D12.04c BioGRID Interaction Datasets
Bähler Lab SPBC12D12.04c Cell Cycle Expression Profile
Bähler Lab SPBC12D12.04c Meiosis/Sporulation Expression Profies
Bähler Lab SPBC12D12.04c Pheromone response/mating expression profiles
Bähler Lab SPBC12D12.04c Environmental stress expression profiles
Bähler Lab SPBC12D12.04c Bähler Lab Transcriptome Viewer
Cyclebase SPBC12D12.04c Cell Cycle Data
PInt SPBC12D12.04c Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPBC12D12.04c Entrez Gene
IntEnz2.7.1.-
EntrezGene2539649protein kinase C (PKC)-like Pck2
Uniprot_genenamePCK2
WikiGene2539649protein kinase C (PKC)-like Pck2
SPD / RIKEN34/34A04Orfeome Localization Data
UniProtKB/SwissProtP36583Protein kinase C-like 2
ModBaseP36583Database of comparative protein structure models
Pfam Protein DomainsP36583Pfam Domain Arrangement
RefSeq PeptideNP_595950protein kinase C (PKC)-like Pck2
RefSeq mRNANM_001021859972h- protein kinase C (PKC)-like Pck2 (pck2), mRNA
European Nucleotide ArchiveAAA35323ENA Protein Mapping
European Nucleotide ArchiveBAA03268ENA Protein Mapping
European Nucleotide ArchiveBAA87128ENA Protein Mapping
European Nucleotide ArchiveCAA22678ENA Protein Mapping
UniParcUPI00001313ECUniProt Archive

Literature for pck2
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013