pck2 (SPBC12D12.04c)


Gene Standard Namepck2 Characterisation Statuspublished
Systematic IDSPBC12D12.04c Feature Typeprotein coding
Synonymspkc1, sts6 Name Descriptionstaurosporine supersensitive
Productprotein kinase C (PKC)-like Pck2 Product Size1016aa, 116.01 kDa
Genomic Location Chromosome II, 2313301-2309126 (4176nt); CDS:2312837-2309787 (3051nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037749
GO:0005515protein bindingIPIrho1PMID:10504305861
IPIrho2PMID:10504305
GO:0004697protein kinase C activityIEA EC:2.7.11.13GO_REF:00000032
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0050732negative regulation of peptidyl-tyrosine phosphorylation1
regulates pmk1IMPPMID:9135147
GO:0050850positive regulation of calcium-mediated signalingIMPPMID:169289596
GO:0032949regulation of alpha-glucan biosynthetic processIMPPMID:111025323
GO:0090334regulation of cell wall (1->3)-beta-D-glucan biosynthetic processIMPPMID:105043056
GO:2000769regulation of establishment or maintenance of cell polarity regulating cell shapeIMPPMID:849119026
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032153cell division siteIDAPMID:16823372295
GO:0030428cell septumIDAPMID:1065190227
GO:0051286cell tipIDAPMID:16823372188
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaypck2+ (wild type)OverexpressionPECO:0000102, PECO:0000126, PECO:0000005PMID:1101684799
FYPO:0002061inviable vegetative cell populationCell growth assaypck2+ (wild type)OverexpressionPECO:0000102, PECO:0000126PMID:91992861315
FYPO:0002161normal growth on Calcofluor WhiteCell growth assaypck2D (partial disruption)Not specifiedPECO:0000102, PECO:0000005PMID:105043052
Cell growth assaypck2+ (wild type)KnockdownPECO:0000102, PECO:0000005PMID:10504305
FYPO:0001192normal growth on cell wall-degrading enzymesCell growth assaypck2+ (wild type)OverexpressionPECO:0000126PMID:146258986
FYPO:0002160normal growth on cilofunginCell growth assaypck2D (partial disruption)Not specifiedPECO:0000102, PECO:0000005PMID:105043051
FYPO:0001164normal growth on glucose carbon sourceCell growth assaypck2+ (wild type)OverexpressionPECO:0000102, PECO:0000150, PECO:0000005, PECO:0000126PMID:11016847145
FYPO:0001420normal vegetative cell population growth rateCell growth assaypck2+ (wild type)KnockdownPMID:1050430538
FYPO:0002162resistance to cilofunginCell growth assaypck2+ (wild type)KnockdownPECO:0000102, PECO:0000005PMID:105043051
FYPO:0000767resistance to staurosporineCell growth assaypck2+ (wild type)OverexpressionPMID:84911907
FYPO:0002720sensitive to beta-glucanaseCell growth assaypck2ΔNullPECO:0000126PMID:146258985
Cell growth assaypck2ΔNullPMID:9199286
FYPO:0000109sensitive to papulacandin BCell growth assaypck2D (partial disruption)Not specifiedPECO:0000102, PECO:0000005PMID:105043054
FYPO:0000271sensitive to salt stressCell growth assaypck2ΔNullPECO:0000102, PECO:0000004, PECO:0000126, PECO:0000207PMID:919928682
FYPO:0000113sensitive to staurosporineCell growth assaysts6-8Not specifiedPECO:0000201, PECO:0000005, PECO:0000102PMID:18992307
Cell growth assaypck2ΔNullPMID:8491190
FYPO:0002060viable vegetative cell populationMicroscopypck2ΔNullPMID:204732893760
Cell growth assaypck2ΔNullPMID:8491190
Microscopypck2ΔNullPECO:0000004, PECO:0000137PMID:23697806
Microscopypck2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000026abnormal cell polarityMicroscopypck2+ (wild type)OverexpressionPMID:84911903
FYPO:0001971abnormal cytokinetic cell separation resulting in chained cellsMicroscopypck2+ (wild type)OverexpressionPMID:105043055
FYPO:0000705abolished protein binding51
affecting rho1Reporter gene assay69-121 (1-68, 122-1017)Not specifiedPMID:10504305
affecting rho1Reporter gene assay362-1017 (1-361)Not specifiedPMID:10504305
affecting rho1Reporter gene assay362-592 (1-361, 593-1017)Not specifiedPMID:10504305
FYPO:0001081decreased cell wall alpha-glucan levelSubstance quantification evidencepck2D (partial disruption)Not specifiedPMID:105043056
FYPO:0002163decreased cell wall thickness during vegetative growthMicroscopypck2D (partial disruption)Not specifiedPECO:0000126PMID:105043052
FYPO:0001645decreased protein binding29
affecting rho1Reporter gene assay69-238 (1-68, 239-1017)Not specifiedPMID:10504305
affecting rho1Reporter gene assay1-121 (122-1017)Not specifiedPMID:10504305
FYPO:0001968increased 1,3-beta-D-glucan synthase activityEnzyme assay datapck2+ (wild type)OverexpressionPMID:105043055
FYPO:0001194increased beta-glucan levelSubstance quantification evidencepck2+ (wild type)OverexpressionPMID:105043053
FYPO:0001084increased cell wall alpha-glucan levelSubstance quantification evidencepck2+ (wild type)OverexpressionPMID:105043055
FYPO:0001035increased cell wall thickness during vegetative growthMicroscopypck2+ (wild type)OverexpressionPECO:0000126PMID:105043056
FYPO:0001198increased cellular calcium levelEnzyme assay datapck2+ (wild type)OverexpressionPECO:0000126, PECO:0000005PMID:110168479
FYPO:0001180increased protein tyrosine phosphorylation6
affecting pmk1Western blot assaypck2ΔNullPMID:9135147
FYPO:0002462inviable branched, elongated vegetative cellMicroscopypck2+ (wild type)OverexpressionPMID:84911908
FYPO:0001489inviable vegetative cellMicroscopypck2ΔNullPECO:0000004, PECO:0000137PMID:236978061189
FYPO:0002157normal 1,3-beta-D-glucan synthase activityEnzyme assay datapck2D (partial disruption)Not specifiedPMID:105043052
FYPO:0001079normal galactomannan levelSubstance quantification evidencepck2+ (wild type)OverexpressionPMID:105043055
FYPO:0000703normal protein binding30
affecting rho1Reporter gene assay1-149 (150-1017)Not specifiedPMID:10504305
affecting rho1Reporter gene assay1-361 (362-1017)Not specifiedPMID:10504305
FYPO:0001315normal vegetative cell morphologyMicroscopypck2+ (wild type)KnockdownPMID:1050430570
FYPO:0000647vegetative cell lysisMicroscopypck2+ (wild type)OverexpressionPECO:0000126, PECO:0000005PMID:1101684743
FYPO:0002110viable bottle-shaped vegetative cellMicroscopypck2D (partial disruption)Not specifiedPECO:0000126, PECO:0000005PMID:105043056
FYPO:0002903viable pear-shaped vegetative cellMicroscopypck2ΔNullPMID:84911901
FYPO:0002380viable spheroid vegetative cellMicroscopypck2ΔNullPECO:0000005, PECO:0000137PMID:2369780637
Microscopypck2ΔNullPMID:8491190
Microscopypck2ΔNullPECO:0000126, PECO:0000005PMID:9199286
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopypck2ΔNullPECO:0000005, PECO:0000137PMID:236978063086
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in cdc42 Rho family GTPase Cdc42 PMID:10504305
FYPO affected by mutation in ksg1 serine/threonine protein kinase Ksg1 PMID:14625898
FYPO affected by mutation in rga2 Rho-type GTPase activating protein Rga2 PMID:18793338
FYPO affected by mutation in rho1 Rho family GTPase Rho1 PMID:10504305
FYPO affected by mutation in rho2 Rho family GTPase Rho2 PMID:10504305
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
123133012309126

UTRs

Region Coordinates Reference
five_prime_UTR2313301..2312838PMID:21511999
three_prime_UTR2309786..2309126PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 684 942 109
PF02185 Pfam IPR011072 HR1 rho-binding repeat 8 72 3
PF02185 Pfam IPR011072 HR1 rho-binding repeat 124 191 3
PF00130 Pfam IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 474 525 3
PF00130 Pfam IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 406 455 3
PF00433 Pfam IPR017892 Protein kinase, C-terminal 963 1006 7
SM00742 SMART IPR011072 HR1 rho-binding repeat 123 189 3
SM00742 SMART IPR011072 HR1 rho-binding repeat 6 69 3
SM00109 SMART IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 474 523 5
SM00109 SMART IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 406 453 5
SM00220 SMART IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain 683 942 73
SM00133 SMART IPR000961 AGC-kinase, C-terminal 943 1005 13
SM00239 SMART IPR000008 C2 domain 212 306 15
PS51285 Prosite Profiles IPR000961 AGC-kinase, C-terminal 943 1013 13
PS50011 Prosite Profiles IPR000719 Protein kinase domain 683 942 110
PS50081 Prosite Profiles IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 473 523 4
PS50081 Prosite Profiles IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 405 453 4
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 689 712 73
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 804 816 97
PS00479 Prosite Patterns IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 406 453 3
PS00479 Prosite Patterns IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding 474 523 3
PTHR24357 HMMPANTHER 76 1016 2
PTHR24357:SF73 HMMPANTHER 76 1016 2
3.30.200.20 Gene3D 677 755 109
2.60.40.150 Gene3D IPR000008 C2 domain 208 300 19
2.60.40.150 Gene3D IPR000008 C2 domain 351 375 19
3.30.60.20 Gene3D 397 466 4
3.30.60.20 Gene3D 467 533 4
1.10.287.160 Gene3D IPR011072 HR1 rho-binding repeat 119 184 3
1.10.510.10 Gene3D 756 954 112
SSF49562 SuperFamily IPR000008 C2 domain 208 299 16
SSF49562 SuperFamily IPR000008 C2 domain 352 375 16
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 680 999 127
SSF57889 SuperFamily 396 455 5
SSF57889 SuperFamily 466 527 5
SSF46585 SuperFamily IPR011072 HR1 rho-binding repeat 117 191 3
Coil ncoils Rabaptin coiled-coil domain 45 66 968
Coil ncoils Rabaptin coiled-coil domain 158 179 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001518C1 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001518
PBO:0001519C2 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001519

Protein Properties

Ave. residue weight 114.18 Da
Charge 26.00
Isoelectric point 8.17
Molecular weight 116.01 kDa
Number of residues 1016
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS86PMID:247631071663
S649PMID:24763107
present during mitotic M phaseS105PMID:21712547
present during mitotic M phaseS988PMID:21712547
present during mitotic M phaseS72PMID:21712547
present during mitotic M phaseS649PMID:21712547
S988PMID:24763107
S27PMID:24763107
S20PMID:24763107
MOD:00047O-phospho-L-threonineT984PMID:21712547682
present during mitotic M phase
T984PMID:24763107
MOD:00696phosphorylated residueNASPMID:195477441915
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
774.84during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
557.48during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
5505during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
5522during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
5153during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
5561during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
4300during GO:0072690PECO:0000012,
PECO:0000005
experimental evidencePMID:16224022
5482during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.8during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
wis1MAP kinase kinase Wis1 Phenotypic SuppressionPMID:9199286
rga4Rho-type GTPase activating protein Rga4 Phenotypic SuppressionPMID:20164182
pmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:17881729
kin1microtubule affinity-regulating kinase Kin1 Synthetic RescuePMID:23294323
Phenotypic Suppression
Phenotypic SuppressionPMID:20624220
art1arrestin family protein Art1 Dosage RescuePMID:20739711
gls2glucosidase II alpha subunit Gls2 Dosage RescuePMID:10504305
rho1Rho family GTPase Rho1 Dosage RescuePMID:10651902
Phenotypic EnhancementPMID:10504305
pek1MAP kinase kinase Pek1 Synthetic RescuePMID:17005909
rho2Rho family GTPase Rho2 Synthetic RescuePMID:17005909
Dosage RescuePMID:11102532
Synthetic Growth Defect
sty1MAP kinase Sty1 Phenotypic SuppressionPMID:9199286
pck1protein kinase C (PKC)-like Pck1 Dosage RescuePMID:15923187
Synthetic LethalityPMID:10504305
Synthetic LethalityPMID:8491190
pmk1MAP kinase Pmk1 Phenotypic EnhancementPMID:8943330
Synthetic RescuePMID:17005909
Dosage RescuePMID:9199286
Phenotypic Suppression
ptc3protein phosphatase 2c homolog 3 Dosage RescuePMID:17881729
pap1transcription factor Pap1/Caf3 Dosage RescuePMID:1899230
ptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:17881729
yam8calcium channel regulatory subunit Yam8 Dosage RescuePMID:11016847
Synthetic Rescue
Synthetic Lethality
cpc2RACK1 ortholog Cpc2 Synthetic Growth DefectPMID:11263963
Phenotypic Enhancement
its31-phosphatidylinositol-4-phosphate 5-kinase Its3 Synthetic LethalityPMID:15923187
ags1alpha glucan synthase Ags1 Dosage LethalityPMID:10087262
Synthetic Growth Defect
sts5RNB-like protein Dosage RescuePMID:8886983
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Dosage RescuePMID:10504305
spk1MAP kinase Spk1 Dosage RescuePMID:1899230
ppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic RescuePMID:9427748
Synthetic RescuePMID:17005909
ksg1serine/threonine protein kinase Ksg1 Dosage RescuePMID:14625898
Synthetic Growth Defect
mkh1MEK kinase (MEKK) Mkh1 Synthetic RescuePMID:17005909
Phenotypic SuppressionPMID:9199286
Synthetic Growth Defect
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rho2Rho family GTPase Rho2 Affinity Capture-WesternPMID:18793338
Two-hybridPMID:10504305
cpc2RACK1 ortholog Cpc2 Reconstituted ComplexPMID:11263963
Affinity Capture-Western
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
mkh1MEK kinase (MEKK) Mkh1 Affinity Capture-WesternPMID:17005909
cdc42Rho family GTPase Cdc42 Two-hybridPMID:10504305
rho1Rho family GTPase Rho1 Reconstituted ComplexPMID:10651902
Two-hybrid
Two-hybridPMID:10504305
External References
Database Identifier Description
NBRP SPBC12D12.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC12D12.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC12D12.04c BioGRID Interaction Datasets
Expression Viewer SPBC12D12.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC12D12.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC12D12.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC12D12.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC12D12.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC12D12.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC12D12.04c Cell Cycle Data
GEO SPBC12D12.04c GEO profiles
PInt SPBC12D12.04c Protein-Protein Interaction Predictor (Bähler Lab)
IntEnz2.7.1.-Integrated relational Enzyme database
Rhea2.7.1.-Annotated reactions database
EntrezGene2539649protein kinase C (PKC)-like Pck2
WikiGene2539649protein kinase C (PKC)-like Pck2
SPD / RIKEN34/34A04Orfeome Localization Data
UniProtKB/SwissProtP36583Protein kinase C-like 2
ModBaseP36583Database of comparative protein structure models
STRINGP36583Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595950protein kinase C (PKC)-like Pck2
RefSeq mRNANM_001021859972h- protein kinase C (PKC)-like Pck2 (pck2), mRNA
European Nucleotide ArchiveAAA35323ENA Protein Mapping
European Nucleotide ArchiveAAA35323.1ENA Protein Mapping
European Nucleotide ArchiveBAA03268ENA Protein Mapping
European Nucleotide ArchiveBAA03268.1ENA Protein Mapping
European Nucleotide ArchiveBAA87128ENA Protein Mapping
European Nucleotide ArchiveBAA87128.1ENA Protein Mapping
European Nucleotide ArchiveCAA22678ENA Protein Mapping
European Nucleotide ArchiveCAA22678.1ENA Protein Mapping
UniParcUPI00001313ECUniProt Archive

Literature for pck2

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014