pck2 (SPBC12D12.04c)


Gene Standard Namepck2 Characterisation Statuspublished
Systematic IDSPBC12D12.04c Feature Typeprotein coding
Synonymspkc1, sts6 Name DescriptionSTaurosporine Supersensitive
Productprotein kinase C (PKC)-like Pck2 Product Size1016aa, 116.01 kDa
Genomic Location Chromosome II, 2313301-2309126 (4176nt); CDS:2312837-2309787 (3051nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding551
Annotation ExtensionEvidenceWith/FromReference
metal ion binding742
Annotation ExtensionEvidenceWith/FromReference
protein binding900
Annotation ExtensionEvidenceWith/FromReference
protein kinase C activity2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
fungal-type cell wall biogenesis65
Annotation ExtensionEvidenceWith/FromReference
intracellular signal transduction206
Annotation ExtensionEvidenceWith/FromReference
negative regulation of peptidyl-tyrosine phosphorylation1
Annotation ExtensionEvidenceWith/FromReference
regulation of alpha-glucan biosynthetic process3
Annotation ExtensionEvidenceWith/FromReference
regulation of cell wall (1->3)-beta-D-glucan biosynthetic process6
Annotation ExtensionEvidenceWith/FromReference
regulation of establishment or maintenance of cell polarity regulating cell shape28
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
barrier septum27
Annotation ExtensionEvidenceWith/FromReference
cell division site318
Annotation ExtensionEvidenceWith/FromReference
cell tip211
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased bipolar indexpck2Δ27
decreased cell population growth on glucose carbon sourcepck2+393
increased monopolar indexpck2Δ39
inviable vegetative cell populationpck2+1468
normal growth during cellular response to salt stresspck2Δ25
normal growth on calcium ionpck2Δ8
normal growth on Calcofluor Whitepck2::LEU25
pck2+
normal growth on cell wall-degrading enzymespck2+11
normal growth on cilofunginpck2::LEU21
normal growth on glucose carbon sourcepck2+318
normal growth on K-252apck2Δ3
normal vegetative cell population growth ratepck2+85
resistance to cilofunginpck2+1
resistance to staurosporinepck2+16
sensitive to benomylpck2Δ37
sensitive to beta-glucanasepck2Δ10
sensitive to latrunculin Apck2Δ39
sensitive to papulacandin Bpck2::LEU24
sensitive to salt stresspck2Δ102
sensitive to staurosporinests6-815
pck2::LEU2
pck2-8
viable vegetative cell populationpck2::LEU23862
pck2Δ

Cell Phenotype

Term NameGenotypesCount
abnormal cell separation after cytokinesis resulting in chained cellspck2+13
abnormal cell wall biogenesispck2Δ40
abnormal vegetative cell polaritypck2+9
abolished protein-protein interaction164
affecting rho1 and pck2362-1017 (1-361)
affecting rho1 and pck2362-592 (1-361, 593-1017)
affecting rho1 and pck269-121 (1-68, 122-1017)
decreased cell wall alpha-glucan levelpck2::LEU26
decreased cell wall thickness during vegetative growthpck2::LEU23
decreased protein-protein interaction109
affecting rho1 and pck269-238 (1-68, 239-1017)
affecting rho1 and pck21-121 (122-1017)
decreased transcription from CDRE promoter in response to micafunginpck2Δ6
decreased transcription from CDRE promoter in response to salt stresspck2Δ6
increased 1,3-beta-D-glucan synthase activitypck2+5
increased beta-glucan levelpck2+8
increased cell wall alpha-glucan levelpck2+10
increased cell wall thickness during vegetative growthpck2+12
increased cellular calcium levelpck2+8
increased protein tyrosine phosphorylation during vegetative growth11
affecting pmk1pck2Δ
inviable branched, elongated vegetative cellpck2+24
inviable vegetative cellpck2Δ1377
irregular cell wall during vegetative growthpck2::LEU26
normal 1,3-beta-D-glucan synthase activitypck2::LEU24
normal galactomannan levelpck2+6
normal protein-protein interaction107
affecting rho1 and pck21-361 (362-1017)
affecting rho1 and pck21-149 (150-1017)
normal vegetative cell morphologypck2+582
vegetative cell lysispck2+98
viable pear-shaped vegetative cellpck2::LEU212
viable spheroid vegetative cellpck2Δ53
pck2::LEU2
viable vegetative cellpck2Δ3680
viable vegetative cell with normal cell morphologypck2Δ3103

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased cell population growthhmg1-1 (W234->opal), pck2Δ
normal growth during cellular response to salt stresspck2Δ, kin1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in ksg1 serine/threonine protein kinase Ksg1
FYPO affected by mutation in pck2 protein kinase C (PKC)-like Pck2
FYPO affected by mutation in rga2 RhoGAP, GTPase activating protein Rga2
FYPO affected by mutation in rho1 Rho family GTPase Rho1
FYPO affected by mutation in rho2 Rho family GTPase Rho2
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2313301..2309126
mRNA2313301..2309126
5' UTR2313301..2312838PMID:21511999
CDS2312837..2309787
3' UTR2309786..2309126PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00130 Pfam IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 406 455 3
PF00130 Pfam IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 474 525 3
PF00433 Pfam IPR017892 Protein kinase, C-terminal 964 1003 7
PF00069 Pfam IPR000719 Protein kinase domain 684 942 108
PF02185 Pfam IPR011072 HR1 rho-binding repeat 125 188 3
PF02185 Pfam IPR011072 HR1 rho-binding repeat 8 66 3
SM00109 SMART IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 406 453 5
SM00109 SMART IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 474 523 5
SM00742 SMART IPR011072 HR1 rho-binding repeat 6 69 3
SM00742 SMART IPR011072 HR1 rho-binding repeat 123 189 3
SM00220 SMART IPR000719 Protein kinase domain 683 942 107
SM00133 SMART IPR000961 AGC-kinase, C-terminal 943 1005 11
SM00239 SMART IPR000008 C2 domain 212 306 12
PS00479 Prosite Patterns IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 474 523 3
PS00479 Prosite Patterns IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 406 453 3
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 804 816 91
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 689 712 70
PS50081 Prosite Profiles IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 405 453 4
PS50081 Prosite Profiles IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 473 523 4
PS50011 Prosite Profiles IPR000719 Protein kinase domain 683 942 108
PS51285 Prosite Profiles IPR000961 AGC-kinase, C-terminal 943 1013 13
PTHR24356:SF203 HMMPANTHER 388 549 2
PTHR24356:SF203 HMMPANTHER 634 1003 2
PTHR24356 HMMPANTHER 388 549 13
PTHR24356 HMMPANTHER 634 1003 13
PTHR24356:SF203 HMMPANTHER 5 45 2
PTHR24356 HMMPANTHER 5 45 13
1.10.510.10 Gene3D Immunoglobulin-like fold 756 954 112
3.30.60.20 Gene3D Chimaerin 467 533 4
3.30.60.20 Gene3D Chimaerin 397 466 4
3.30.200.20 Gene3D Immunoglobulin-like fold 677 755 111
1.10.287.160 Gene3D IPR011072 HR1 rho-binding repeat 119 184 3
2.60.40.150 Gene3D IPR000008 C2 domain 351 375 19
2.60.40.150 Gene3D IPR000008 C2 domain 208 300 19
SSF49562 SuperFamily IPR000008 C2 domain 352 375 16
SSF49562 SuperFamily IPR000008 C2 domain 208 299 16
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 680 999 127
SSF46585 SuperFamily IPR011072 HR1 rho-binding repeat 117 191 3
SSF57889 SuperFamily 396 455 5
SSF57889 SuperFamily 466 527 5
Coil ncoils Predicted coiled-coil protein (DUF2205) 45 65 1049
Coil ncoils Predicted coiled-coil protein (DUF2205) 158 178 1049

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001518C1 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001518
PBO:0001519C2 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001519

Protein Properties

Ave. residue weight 114.18 Da
Charge 26.00
Codon Adaptation Index 0.46
Isoelectric point 8.17
Molecular weight 116.01 kDa
Number of residues 1016
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2293
present during mitotic M phaseS72
present during mitotic M phaseS105
present during mitotic M phaseS649
present during mitotic M phaseS988
S20, S27, S86, S88, S95, S399, S649, S910, S912, S988
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S72 PMID:21712547
present during mitotic M phase experimental evidence S105 PMID:21712547
present during mitotic M phase experimental evidence S649 PMID:21712547
present during mitotic M phase experimental evidence S988 PMID:21712547
IDA S86 PMID:25720772
experimental evidence S27 PMID:24763107
IDA S95 PMID:25720772
experimental evidence S86 PMID:24763107
experimental evidence S649 PMID:24763107
IDA S988 PMID:25720772
IDA S88 PMID:25720772
experimental evidence S20 PMID:24763107
IDA S910 PMID:25720772
IDA S912 PMID:25720772
IDA S399 PMID:25720772
experimental evidence S988 PMID:24763107
O-phospho-L-threonine 1087
present during mitotic M phaseT984
T984
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T984 PMID:21712547
experimental evidence T984 PMID:24763107
IDA T984 PMID:25720772
phosphorylated residue 2515
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
O-phosphorylated residueS92,S95, T984,S988 2461
Annotation ExtensionEvidenceResidueReference
IDA T984,S988 PMID:25720772
IDA S92,S95 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pck2 (SPBC12D12.04c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5505during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5561during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5482during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5522during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4300during GO:0072690PECO:0000137,
PECO:0000005
single cellexperimental evidencePMID:16224022
5153during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
557.48during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
774.84during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.8during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4608
conserved in eukaryotes4516
conserved in metazoa3498
conserved in vertebrates3473
conserved in eukaryotes only2525
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC12D12.04c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withrho1Rho family GTPase Rho1 Two-hybridPMID:10651902
binds activation domain construct withcdc42Rho family GTPase Cdc42 Two-hybridPMID:10504305
binds activation domain construct withrho2Rho family GTPase Rho2 Two-hybridPMID:10504305
forms complex withcpc2RACK1 ortholog Cpc2 Reconstituted ComplexPMID:11263963
forms complex withrho1Rho family GTPase Rho1 Reconstituted ComplexPMID:10651902
affinity captured bymkh1MEK kinase (MEKK) Mkh1 Affinity Capture-WesternPMID:17005909
affinity captured bycpc2RACK1 ortholog Cpc2 Affinity Capture-WesternPMID:11263963
affinity captured byrho2Rho family GTPase Rho2 Affinity Capture-WesternPMID:18793338
binds DNA-binding domain construct withgef1Cdc42 RhoGEF Gef1 Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC12D12.04c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withhmg13-hydroxy-3-methylglutaryl-CoA reductase Hmg1 Synthetic Growth DefectPMID:19486165
synthetic growth defect withcpc2RACK1 ortholog Cpc2 Synthetic Growth DefectPMID:11263963
synthetic growth defect withags1alpha glucan synthase Ags1 Synthetic Growth DefectPMID:10087262
synthetic growth defect withrho1Rho family GTPase Rho1 Synthetic Growth DefectPMID:24498240
synthetic growth defect withrho2Rho family GTPase Rho2 Synthetic Growth DefectPMID:11102532
synthetic growth defect withmkh1MEK kinase (MEKK) Mkh1 Synthetic Growth DefectPMID:9199286
synthetic growth defect withksg1serine/threonine protein kinase Ksg1 Synthetic Growth DefectPMID:14625898
rescued by overexpression ofpck1protein kinase C (PKC)-like Pck1 Dosage RescuePMID:8943330
rescued by overexpression ofpap1transcription factor Pap1/Caf3 Dosage RescuePMID:1899230
rescued by overexpression ofspk1MAP kinase Spk1 Dosage RescuePMID:1899230
rescued by overexpression ofpmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:17881729
rescued by overexpression ofgls2glucosidase II alpha subunit Gls2 Dosage RescuePMID:10504305
rescued by overexpression ofbgs1primary septum and spore wall linear 1,3-beta-glucan synthase catalytic subunit Bgs1 Dosage RescuePMID:10504305
rescued by overexpression ofpmk1MAP kinase Pmk1 Dosage RescuePMID:9199286
rescued by overexpression ofptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:17881729
rescued by overexpression ofptc3protein phosphatase 2c homolog 3 Dosage RescuePMID:17881729
synthetic lethal withpck1protein kinase C (PKC)-like Pck1 Synthetic LethalityPMID:10504305
synthetic lethal withyam8stretch-activated calcium ion channel Yam8 Synthetic LethalityPMID:11016847
synthetic lethal withits31-phosphatidylinositol-4-phosphate 5-kinase Its3 Synthetic LethalityPMID:15923187
overexpression rescuesyam8stretch-activated calcium ion channel Yam8 Dosage RescuePMID:11016847
overexpression rescuesrho2Rho family GTPase Rho2 Dosage RescuePMID:11102532
overexpression rescuesart1arrestin family protein Art1 Dosage RescuePMID:20739711
overexpression rescuesrho1Rho family GTPase Rho1 Dosage RescuePMID:10651902
overexpression rescuesksg1serine/threonine protein kinase Ksg1 Dosage RescuePMID:14625898
overexpression rescuessts5RNB-like protein Dosage RescuePMID:8886983
synthetically rescued bymkh1MEK kinase (MEKK) Mkh1 Synthetic RescuePMID:17005909
synthetically rescued bypek1MAP kinase kinase Pek1 Synthetic RescuePMID:17005909
synthetically rescued bypmk1MAP kinase Pmk1 Synthetic RescuePMID:17005909
synthetically rescued byyam8stretch-activated calcium ion channel Yam8 Synthetic RescuePMID:11016847
rescuesmkh1MEK kinase (MEKK) Mkh1 Phenotypic SuppressionPMID:9199286
rescueskin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:23294323
rescuesrga4RhoGAP, GTPase activating protein Rga4 Phenotypic SuppressionPMID:20164182
enhances phenotype ofcpc2RACK1 ortholog Cpc2 Phenotypic EnhancementPMID:11263963
enhances phenotype ofpmk1MAP kinase Pmk1 Phenotypic EnhancementPMID:8943330
synthetically rescueskin1microtubule affinity-regulating kinase Kin1 Synthetic RescuePMID:23294323
synthetically rescuesrho2Rho family GTPase Rho2 Synthetic RescuePMID:17005909
synthetically rescuesppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic RescuePMID:9427748
inviable in presence of overexpressedags1alpha glucan synthase Ags1 Dosage LethalityPMID:10087262
rescued bysty1MAP kinase Sty1 Phenotypic SuppressionPMID:9199286
rescued bypmk1MAP kinase Pmk1 Phenotypic SuppressionPMID:9199286
rescued byrho2Rho family GTPase Rho2 Phenotypic SuppressionPMID:24498240
rescued bywis1MAP kinase kinase Wis1 Phenotypic SuppressionPMID:9199286
External References
Database Identifier Description
NBRP SPBC12D12.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC12D12.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC12D12.04c BioGRID Interaction Datasets
Expression Viewer SPBC12D12.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC12D12.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC12D12.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC12D12.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC12D12.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC12D12.04c Transcriptome Viewer (Bähler Lab)
GEO SPBC12D12.04c GEO profiles
PInt SPBC12D12.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC12D12.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC12D12.04c Fission yeast phenotypic data & analysis
Cyclebase SPBC12D12.04c.1 Cell Cycle Data
IntEnz2.7.1.-Integrated relational Enzyme database
Rhea2.7.1.-Annotated reactions database
SPD / RIKEN34/34A04Orfeome Localization Data
UniProtKB/SwissProtP36583Protein kinase C-like 2
ModBaseP36583Database of comparative protein structure models
STRINGP36583Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595950protein kinase C (PKC)-like Pck2
RefSeq mRNANM_001021859972h- protein kinase C (PKC)-like Pck2 (pck2), mRNA
European Nucleotide ArchiveAAA35323.1ENA Protein Mapping
European Nucleotide ArchiveBAA03268.1ENA Protein Mapping
European Nucleotide ArchiveBAA87128.1ENA Protein Mapping
European Nucleotide ArchiveCAA22678.1ENA Protein Mapping
UniParcUPI00001313ECUniProt Archive

Literature for pck2

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017