pck2 (SPBC12D12.04c)


Gene Standard Namepck2 Characterisation Statuspublished
Systematic IDSPBC12D12.04c Feature Typeprotein coding
Synonymspkc1, sts6 Name Descriptionstaurosporine supersensitive
Productprotein kinase C (PKC)-like Pck2 Product Size1016aa, 116.01 kDa
Genomic Location Chromosome II, 2313301-2309126 (4176nt); CDS:2312837-2309787 (3051nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
metal ion binding747
Annotation ExtensionEvidenceWith/FromReference
protein binding848
Annotation ExtensionEvidenceWith/FromReference
protein kinase C activity2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
fungal-type cell wall biogenesis70
Annotation ExtensionEvidenceWith/FromReference
negative regulation of peptidyl-tyrosine phosphorylation1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of calcium-mediated signaling6
Annotation ExtensionEvidenceWith/FromReference
regulation of alpha-glucan biosynthetic process3
Annotation ExtensionEvidenceWith/FromReference
regulation of cell wall (1->3)-beta-D-glucan biosynthetic process6
Annotation ExtensionEvidenceWith/FromReference
regulation of establishment or maintenance of cell polarity regulating cell shape25
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site299
Annotation ExtensionEvidenceWith/FromReference
cell septum27
Annotation ExtensionEvidenceWith/FromReference
cell tip191
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased bipolar indexpck2ΔNull27
decreased cell population growth on glucose carbon sourcepck2+ (wild type)Overexpression154
increased monopolar indexpck2ΔNull35
inviable vegetative cell populationpck2+ (wild type)Overexpression1427
normal growth during cellular response to salt stresspck2ΔNull13
normal growth on calcium ionpck2ΔNull5
normal growth on Calcofluor Whitepck2+ (wild type)Knockdown1
pck2D (partial disruption)Not specified
normal growth on cell wall-degrading enzymespck2+ (wild type)Overexpression8
normal growth on cilofunginpck2D (partial disruption)Not specified1
normal growth on glucose carbon sourcepck2+ (wild type)Overexpression189
normal vegetative cell population growth ratepck2+ (wild type)Knockdown57
resistance to cilofunginpck2+ (wild type)Knockdown1
resistance to staurosporinepck2+ (wild type)Overexpression8
sensitive to beta-glucanasepck2ΔNull7
sensitive to papulacandin Bpck2D (partial disruption)Not specified4
sensitive to salt stresspck2ΔNull86
sensitive to staurosporinepck2-8Not specified9
pck2ΔNull
sts6-8Not specified
viable vegetative cell populationpck2ΔNull3781

Cell Phenotype

Term NameAlleleExpressionCount
abnormal cell polaritypck2+ (wild type)Overexpression5
abnormal cell wall biogenesispck2ΔNull23
abnormal cytokinetic cell separation resulting in chained cellspck2+ (wild type)Overexpression7
abolished protein binding68
affecting rho1 and pck2362-1017 (1-361)Not specified
affecting rho1 and pck2362-592 (1-361, 593-1017)Not specified
affecting rho1 and pck269-121 (1-68, 122-1017)Not specified
decreased cell wall alpha-glucan levelpck2D (partial disruption)Not specified6
decreased cell wall thickness during vegetative growthpck2D (partial disruption)Not specified3
decreased protein binding51
affecting rho1 and pck21-121 (122-1017)Not specified
affecting rho1 and pck269-238 (1-68, 239-1017)Not specified
increased 1,3-beta-D-glucan synthase activitypck2+ (wild type)Overexpression5
increased beta-glucan levelpck2+ (wild type)Overexpression3
increased cell wall alpha-glucan levelpck2+ (wild type)Overexpression5
increased cell wall thickness during vegetative growthpck2+ (wild type)Overexpression9
increased cellular calcium levelpck2+ (wild type)Overexpression9
increased protein tyrosine phosphorylation10
affecting pmk1pck2ΔNull
inviable branched, elongated vegetative cellpck2+ (wild type)Overexpression9
inviable vegetative cellpck2ΔNull1268
irregular cell wall during vegetative growthpck2D (partial disruption)Not specified5
normal 1,3-beta-D-glucan synthase activitypck2D (partial disruption)Not specified2
normal galactomannan levelpck2+ (wild type)Overexpression5
normal protein binding49
affecting rho1 and pck21-149 (150-1017)Not specified
affecting rho1 and pck21-361 (362-1017)Not specified
normal vegetative cell morphologypck2+ (wild type)Knockdown3580
vegetative cell lysispck2+ (wild type)Overexpression82
viable pear-shaped vegetative cellpck2ΔNull12
viable spheroid vegetative cellpck2ΔNull47
viable vegetative cell with normal cell morphologypck2ΔNull3092
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in ksg1 serine/threonine protein kinase Ksg1 PMID:14625898
FYPO affected by mutation in pck2 protein kinase C (PKC)-like Pck2 PMID:10504305
FYPO affected by mutation in rga2 Rho-type GTPase activating protein Rga2 PMID:18793338
FYPO affected by mutation in rho1 Rho family GTPase Rho1 PMID:10504305
FYPO affected by mutation in rho2 Rho family GTPase Rho2 PMID:10504305
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
123133012309126

UTRs

Region Coordinates Reference
five_prime_UTR2313301..2312838PMID:21511999
three_prime_UTR2309786..2309126PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02185 Pfam IPR011072 HR1 rho-binding repeat 124 191 3
PF02185 Pfam IPR011072 HR1 rho-binding repeat 8 72 3
PF00069 Pfam IPR000719 Protein kinase domain 684 942 109
PF00433 Pfam IPR017892 Protein kinase, C-terminal 963 1006 7
PF00130 Pfam IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 474 525 3
PF00130 Pfam IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 406 455 3
SM00109 SMART IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 406 453 5
SM00109 SMART IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 474 523 5
SM00239 SMART IPR000008 C2 domain 212 306 12
SM00133 SMART IPR000961 AGC-kinase, C-terminal 943 1005 11
SM00742 SMART IPR011072 HR1 rho-binding repeat 6 69 3
SM00742 SMART IPR011072 HR1 rho-binding repeat 123 189 3
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 683 942 107
PS50081 Prosite Profiles IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 473 523 4
PS50081 Prosite Profiles IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 405 453 4
PS51285 Prosite Profiles IPR000961 AGC-kinase, C-terminal 943 1013 13
PS50011 Prosite Profiles IPR000719 Protein kinase domain 683 942 110
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 804 816 97
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 689 712 73
PS00479 Prosite Patterns IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 474 523 3
PS00479 Prosite Patterns IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 406 453 3
PTHR24357 HMMPANTHER 76 1016 2
PTHR24357:SF73 HMMPANTHER 76 1016 2
1.10.510.10 Gene3D 756 954 112
2.60.40.150 Gene3D IPR000008 C2 domain 351 375 19
2.60.40.150 Gene3D IPR000008 C2 domain 208 300 19
3.30.200.20 Gene3D 677 755 111
3.30.60.20 Gene3D 397 466 4
3.30.60.20 Gene3D 467 533 4
1.10.287.160 Gene3D IPR011072 HR1 rho-binding repeat 119 184 3
SSF57889 SuperFamily 396 455 5
SSF57889 SuperFamily 466 527 5
SSF46585 SuperFamily IPR011072 HR1 rho-binding repeat 117 191 3
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 680 999 127
SSF49562 SuperFamily IPR000008 C2 domain 208 299 16
SSF49562 SuperFamily IPR000008 C2 domain 352 375 16
Coil ncoils Rabaptin coiled-coil domain 158 179 971
Coil ncoils Rabaptin coiled-coil domain 45 66 971

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001518C1 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001518
PBO:0001519C2 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001519

Protein Properties

Ave. residue weight 114.18 Da
Charge 26.00
Isoelectric point 8.17
Molecular weight 116.01 kDa
Number of residues 1016
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS72
present during mitotic M phaseS105
S20, S27, S86, S649, S988
present during mitotic M phaseS988
present during mitotic M phaseS649
Annotation ExtensionEvidenceResidueReference
experimental evidence S20 PMID:24763107
experimental evidence S27 PMID:24763107
present during mitotic M phase experimental evidence S72 PMID:21712547
experimental evidence S86 PMID:24763107
present during mitotic M phase experimental evidence S105 PMID:21712547
experimental evidence S649 PMID:24763107
present during mitotic M phase experimental evidence S649 PMID:21712547
experimental evidence S988 PMID:24763107
present during mitotic M phase experimental evidence S988 PMID:21712547
O-phospho-L-threonineT984 693
present during mitotic M phaseT984
Annotation ExtensionEvidenceResidueReference
experimental evidence T984 PMID:24763107
present during mitotic M phase experimental evidence T984 PMID:21712547
phosphorylated residue 1922
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5505during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5561during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5482during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5522during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4300during GO:0072690PECO:0000005,
PECO:0000012
single cellexperimental evidencePMID:16224022
5153during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
557.48during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
774.84during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.8during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
rho2Rho family GTPase Rho2 Affinity Capture-WesternPMID:18793338
Two-hybridPMID:10504305
cpc2RACK1 ortholog Cpc2 Affinity Capture-WesternPMID:11263963
Reconstituted Complex
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
mkh1MEK kinase (MEKK) Mkh1 Affinity Capture-WesternPMID:17005909
cdc42Rho family GTPase Cdc42 Two-hybridPMID:10504305
rho1Rho family GTPase Rho1 Two-hybridPMID:10651902
Reconstituted Complex
Two-hybridPMID:10504305
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
wis1MAP kinase kinase Wis1 Phenotypic SuppressionPMID:9199286
rga4Rho-type GTPase activating protein Rga4 Phenotypic SuppressionPMID:20164182
pmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:17881729
kin1microtubule affinity-regulating kinase Kin1 Synthetic RescuePMID:23294323
Phenotypic Suppression
Phenotypic SuppressionPMID:20624220
art1arrestin family protein Art1 Dosage RescuePMID:20739711
gls2glucosidase II alpha subunit Gls2 Dosage RescuePMID:10504305
rho1Rho family GTPase Rho1 Dosage RescuePMID:10651902
Synthetic Growth DefectPMID:24498240
pek1MAP kinase kinase Pek1 Synthetic RescuePMID:17005909
rho2Rho family GTPase Rho2 Phenotypic SuppressionPMID:24498240
Synthetic RescuePMID:17005909
Synthetic Growth DefectPMID:11102532
Dosage Rescue
sty1MAP kinase Sty1 Phenotypic SuppressionPMID:9199286
pck1protein kinase C (PKC)-like Pck1 Dosage RescuePMID:15923187
Dosage RescuePMID:8943330
Synthetic LethalityPMID:10504305
Synthetic LethalityPMID:8491190
pmk1MAP kinase Pmk1 Phenotypic EnhancementPMID:8943330
Synthetic RescuePMID:17005909
Phenotypic SuppressionPMID:9199286
Dosage Rescue
ptc3protein phosphatase 2c homolog 3 Dosage RescuePMID:17881729
pap1transcription factor Pap1/Caf3 Dosage RescuePMID:1899230
ptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:17881729
yam8stretch-activated calcium channel Yam8 Synthetic RescuePMID:11016847
Synthetic Lethality
Dosage Rescue
cpc2RACK1 ortholog Cpc2 Synthetic Growth DefectPMID:11263963
Phenotypic Enhancement
its31-phosphatidylinositol-4-phosphate 5-kinase Its3 Synthetic LethalityPMID:15923187
ags1alpha glucan synthase Ags1 Synthetic Growth DefectPMID:10087262
Dosage Lethality
sts5RNB-like protein Dosage RescuePMID:8886983
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Dosage RescuePMID:10504305
spk1MAP kinase Spk1 Dosage RescuePMID:1899230
ppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic RescuePMID:9427748
Synthetic RescuePMID:17005909
ksg1serine/threonine protein kinase Ksg1 Dosage RescuePMID:14625898
Synthetic Growth Defect
mkh1MEK kinase (MEKK) Mkh1 Synthetic RescuePMID:17005909
Synthetic Growth DefectPMID:9199286
Phenotypic Suppression
External References
Database Identifier Description
NBRP SPBC12D12.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC12D12.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC12D12.04c BioGRID Interaction Datasets
Expression Viewer SPBC12D12.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC12D12.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC12D12.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC12D12.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC12D12.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC12D12.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC12D12.04c Cell Cycle Data
GEO SPBC12D12.04c GEO profiles
PInt SPBC12D12.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC12D12.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC12D12.04c Fission yeast phenotypic data & analysis
IntEnz2.7.1.-Integrated relational Enzyme database
Rhea2.7.1.-Annotated reactions database
SPD / RIKEN34/34A04Orfeome Localization Data
UniProtKB/SwissProtP36583Protein kinase C-like 2
ModBaseP36583Database of comparative protein structure models
STRINGP36583Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595950protein kinase C (PKC)-like Pck2
RefSeq mRNANM_001021859972h- protein kinase C (PKC)-like Pck2 (pck2), mRNA
European Nucleotide ArchiveAAA35323.1ENA Protein Mapping
European Nucleotide ArchiveBAA03268.1ENA Protein Mapping
European Nucleotide ArchiveBAA87128.1ENA Protein Mapping
European Nucleotide ArchiveCAA22678.1ENA Protein Mapping
UniParcUPI00001313ECUniProt Archive

Literature for pck2

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015