ptr2 (SPBC13A2.04c)


Gene Standard Nameptr2 Characterisation Statuspublished
Systematic IDSPBC13A2.04c Feature Typeprotein coding
Synonyms Name Description
ProductPTR family peptide transmembrane transporter Ptr2 Product Size618aa, 68.50 kDa
Genomic Location Chromosome II, 3408492-3405575 (2918nt); CDS:3407692-3405836 (1857nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
dipeptide transmembrane transporter activity3
Annotation ExtensionEvidenceWith/FromReference
dipeptide transporter activity3
Annotation ExtensionEvidenceWith/FromReference
tripeptide transporter activity3
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
dipeptide transmembrane transport4
Annotation ExtensionEvidenceWith/FromReference
tripeptide transmembrane transport5
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site305
Annotation ExtensionEvidenceWith/FromReference
fungal-type vacuole133
Annotation ExtensionEvidenceWith/FromReference
integral component of plasma membrane49
Annotation ExtensionEvidenceWith/FromReference
plasma membrane of cell tip11
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
abolished cell population growth on dipeptide nitrogen sourceptr2ΔNull2
viable vegetative cell populationptr2ΔNull3792

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologyptr2ΔNull3092
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in tsc1 hamartin PMID:17556368
FYPO affected by mutation in tsc2 tuberin PMID:17556368
FYPO affected by mutation in ubr11 UBR ubiquitin-protein ligase E3 Ubr11 PMID:22226946
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3408492..3405575
mRNA3408492..3405575
5' UTR3408492..3407693PMID:21511999
CDS3407692..3405836
3' UTR3405835..3405575AU011140
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00854 Pfam IPR000109 Proton-dependent oligopeptide transporter family 158 519 1
TMhelix TMHMM 248 267 959
TMhelix TMHMM 433 455 959
TMhelix TMHMM 272 294 959
TMhelix TMHMM 363 385 959
TMhelix TMHMM 513 535 959
TMhelix TMHMM 479 501 959
TMhelix TMHMM 539 561 959
TMhelix TMHMM 189 211 959
TMhelix TMHMM 162 184 959
TMhelix TMHMM 398 420 959
PS01023 Prosite Patterns IPR018456 PTR2 family proton/oligopeptide symporter, conserved site 247 259 1
PS01022 Prosite Patterns IPR018456 PTR2 family proton/oligopeptide symporter, conserved site 147 171 1
PTHR11654:SF88 HMMPANTHER 1 613 1
PTHR11654 HMMPANTHER IPR000109 Proton-dependent oligopeptide transporter family 1 613 1
1.20.1250.20 Gene3D 402 571 72
SSF103473 SuperFamily IPR020846 Major facilitator superfamily domain 343 564 76
SSF103473 SuperFamily IPR020846 Major facilitator superfamily domain 82 305 76

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 110.84 Da
Charge -5.00
Isoelectric point 5.22
Molecular weight 68.50 kDa
Number of residues 618
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS601 1672
present during mitotic M phaseS43
present during mitotic M phaseS44
present during mitotic M phaseS22
present during mitotic M phase, cellular response to thiabendazoleS25
present during mitotic M phaseS596
present during mitotic M phaseS601
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S22 PMID:21712547
present during mitotic M phase experimental evidence S25 PMID:21712547
present during cellular response to thiabendazole IDA S25 PMID:18257517
present during mitotic M phase experimental evidence S43 PMID:21712547
present during mitotic M phase experimental evidence S44 PMID:21712547
present during mitotic M phase experimental evidence S596 PMID:21712547
experimental evidence S601 PMID:24763107
present during mitotic M phase experimental evidence S601 PMID:21712547
O-phospho-L-threonine 698
present during mitotic M phaseT45
present during mitotic M phaseT38
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T38 PMID:21712547
present during mitotic M phase experimental evidence T45 PMID:21712547
O4'-phospho-L-tyrosine 86
present during mitotic M phaseY603
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence Y603 PMID:21712547
phosphorylated residue 1926
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
920.94during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1563.88during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
conserved in metazoa3430
conserved in vertebrates3405
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byfar8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withago1argonaute Negative GeneticPMID:22681890
negative genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
positive genetic interaction withamk2AMP-activated protein kinase beta subunit Amk2 Positive GeneticPMID:22681890
negative genetic interaction withcdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
negative genetic interaction withcph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
negative genetic interaction withdad1DASH complex subunit Dad1 Negative GeneticPMID:22681890
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:22681890
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:22681890
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:22681890
negative genetic interaction withefc25Ras1 guanyl-nucleotide exchange factor Efc25 Negative GeneticPMID:22681890
negative genetic interaction witherg5C-22 sterol desaturase Erg5 Negative GeneticPMID:22681890
negative genetic interaction withgma12alpha-1,2-galactosyltransferase Gma12 Negative GeneticPMID:22681890
negative genetic interaction withhrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
synthetic growth defect withisp4OPT oligopeptide transmembrane transporter family Isp4 Synthetic Growth DefectPMID:22226946
positive genetic interaction withiwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withmcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
negative genetic interaction withmis4adherin, cohesin loading factor Mis4 Negative GeneticPMID:23050226
negative genetic interaction withmkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
positive genetic interaction withmph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
positive genetic interaction withmug80cyclin Clg1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
negative genetic interaction withppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpst2Clr6 histone deacetylase complex subunit Pst2 Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
negative genetic interaction withrcd1RNA-binding protein, CCR4-NOT complex subunit Rcd1 Negative GeneticPMID:22681890
negative genetic interaction withrga3Rho-type GTPase activating protein Rga3 Negative GeneticPMID:22681890
negative genetic interaction withrpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:22681890
negative genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsen1ATP-dependent 5' to 3' DNA/RNA helicase Sen1 Negative GeneticPMID:22681890
positive genetic interaction withset1histone lysine methyltransferase Set1 Positive GeneticPMID:22681890
negative genetic interaction withSPAC17H9.13cglutamate 5-kinase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC21C3.03ABC1 kinase family protein Negative GeneticPMID:22681890
positive genetic interaction withSPBC713.07cvacuolar polyphosphatase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
negative genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
negative genetic interaction withsty1MAP kinase Sty1 Negative GeneticPMID:22681890
negative genetic interaction withsum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:22681890
positive genetic interaction withswd1Set1C complex subunit Swd1 Positive GeneticPMID:22681890
positive genetic interaction withtif38translation initiation factor eIF3h (p40) Positive GeneticPMID:22681890
negative genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtsc1hamartin Negative GeneticPMID:22681890
negative genetic interaction withtsc2tuberin Negative GeneticPMID:22681890
overexpression rescuesubr11UBR ubiquitin-protein ligase E3 Ubr11 Dosage RescuePMID:22226946
negative genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
negative genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC13A2.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC13A2.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC13A2.04c BioGRID Interaction Datasets
Expression Viewer SPBC13A2.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC13A2.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC13A2.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC13A2.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC13A2.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC13A2.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC13A2.04c Cell Cycle Data
GEO SPBC13A2.04c GEO profiles
PInt SPBC13A2.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC13A2.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC13A2.04c Fission yeast phenotypic data & analysis
SPD / RIKEN49/49C11Orfeome Localization Data
UniProtKB/SwissProtQ9P380Probable peptide transporter ptr2
ModBaseQ9P380Database of comparative protein structure models
STRINGQ9P380Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596417PTR family peptide transporter (predicted)
RefSeq mRNANM_001022336972h- PTR family peptide transporter (predicted) (SPBC13A2.04c), mRNA
European Nucleotide ArchiveU01171ENA EMBL mapping
European Nucleotide ArchiveBAA87178.1ENA Protein Mapping
European Nucleotide ArchiveCAB99397.1ENA Protein Mapping
UniParcUPI0000069A40UniProt Archive

Literature for ptr2

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015