brf1 (SPBC13E7.10c)

Gene Standard Namebrf1 Characterisation Statuspublished
Systematic IDSPBC13E7.10c Feature Typeprotein coding
SynonymsSPBC30D10.20 Name Description
Producttranscription factor TFIIIB complex subunit Brf1 Product Size492aa, 55.80 kDa
Genomic Location Chromosome II, 3058618-3056037 (2582nt); CDS:3058456-3056760 (1697nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
zinc ion binding249
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
transcription factor TFIIIB complex3
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopybrf1ΔNullPECO:0000005, PECO:0000137PMID:236978061338

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopybrf1ΔNullPECO:0000005, PECO:0000137PMID:23697806686
FYPO:0003529inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells87
penetrance FYPO_EXT:0000001Microscopybrf1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0000310inviable after spore germination, without cell division, with normal germ tube morphologyMicroscopybrf1ΔNullPECO:0000005, PECO:0000137PMID:23697806303
FYPO:0002151inviable sporeMicroscopybrf1ΔNullPECO:0000005, PECO:0000137PMID:23697806465
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07741 Pfam IPR011665 Brf1-like TBP-binding 391 487 1
PF00382 Pfam IPR013150 Transcription factor TFIIB, cyclin-like domain 89 156 2
PF00382 Pfam IPR013150 Transcription factor TFIIB, cyclin-like domain 183 256 2
PF08271 Pfam IPR013137 Zinc finger, TFIIB-type 1 42 2
SM00385 SMART IPR013763 Cyclin-like 87 168 13
SM00385 SMART IPR013763 Cyclin-like 181 265 13
PS51134 Prosite Profiles IPR013137 Zinc finger, TFIIB-type 1 30 2
PTHR11618:SF4 HMMPANTHER 1 492 1
PTHR11618 HMMPANTHER IPR000812 Transcription factor TFIIB 1 492 2
1.10.472.10 Gene3D IPR013763 Cyclin-like 75 176 13
1.10.472.10 Gene3D IPR013763 Cyclin-like 177 267 13 Gene3D 3 42 7 Gene3D 379 429 1
SSF47954 SuperFamily IPR013763 Cyclin-like 84 175 15
SSF47954 SuperFamily IPR013763 Cyclin-like 177 264 15
SSF57783 SuperFamily 2 45 8
PR00685 PRINTS IPR000812 Transcription factor TFIIB 129 148 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 219 235 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 15 35 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 251 265 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.41 Da
Charge -2.50
Isoelectric point 6.01
Molecular weight 55.80 kDa
Number of residues 492

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS315PMID:217125471670
present during mitotic M phase
MOD:00047O-phospho-L-threonineexperimental evidenceT320PMID:21712547692
present during mitotic M phase
experimental evidenceT344PMID:24763107
MOD:00048O4'-phospho-L-tyrosineexperimental evidenceY313PMID:2171254785
present during mitotic M phase
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
278.92during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.93during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.64during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
gene structure updated PMID:21511999432
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in archaea237

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
rpc25DNA-directed RNA polymerase III complex subunit Rpc25 Affinity Capture-WesternPMID:19910488
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
pir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
External References
Database Identifier Description
NBRP SPBC13E7.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC13E7.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC13E7.10c BioGRID Interaction Datasets
Expression Viewer SPBC13E7.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC13E7.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC13E7.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC13E7.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC13E7.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC13E7.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC13E7.10c Cell Cycle Data
GEO SPBC13E7.10c GEO profiles
PInt SPBC13E7.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC13E7.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC13E7.10c Fission yeast phenotypic data & analysis
SPD / RIKEN40/40G02Orfeome Localization Data
UniProtKB/SwissProtQ9P6R0Transcription factor IIIB 60 kDa subunit
ModBaseQ9P6R0Database of comparative protein structure models
STRINGQ9P6R0Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596265transcription factor TFIIIB complex subunit Brf1
European Nucleotide ArchiveCAB89885.2ENA Protein Mapping
UniParcUPI000228F4DFUniProt Archive

Literature for brf1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014