brf1 (SPBC13E7.10c)

Gene Standard Namebrf1 Characterisation Statuspublished
Systematic IDSPBC13E7.10c Feature Typeprotein coding
SynonymsSPBC30D10.20 Name Description
Producttranscription factor TFIIIB complex subunit Brf1 Product Size492aa, 55.80 kDa
Genomic Location Chromosome II, 3058618-3056037 (2582nt); CDS:3058456-3056760 (1697nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
zinc ion binding239
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
transcription factor TFIIIB complex3
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationbrf1Δ1451

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cyclebrf1Δ877
inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cellsbrf1Δ88
inviable after spore germination, without cell division, with normal germ tube morphologybrf1Δ303
inviable sporebrf1Δ476
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3058618..3058392, 3058330..3058218, 3058060..3056371, 3056264..3056037
Intron3058391..3058331, 3058217..3058061, 3056370..3056265
5' UTR3058618..3058457PMID:21511999
CDS3058456..3058392, 3058330..3058218, 3058060..3056760
3' UTR3056759..3056371, 3056264..3056037PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00382 Pfam IPR013150 Transcription factor TFIIB, cyclin-like domain 89 156 1
PF00382 Pfam IPR013150 Transcription factor TFIIB, cyclin-like domain 183 256 1
PF08271 Pfam IPR013137 Zinc finger, TFIIB-type 2 42 1
PF07741 Pfam IPR011665 Brf1, TBP-binding domain 391 486 1
SM00385 SMART IPR013763 Cyclin-like 181 265 13
SM00385 SMART IPR013763 Cyclin-like 87 168 13
PS51134 Prosite Profiles IPR013137 Zinc finger, TFIIB-type 1 30 2
PTHR11618 HMMPANTHER IPR000812 Transcription factor TFIIB 1 488 2
PTHR11618:SF4 HMMPANTHER IPR029529 Transcription factor IIIB subunit Brf1 1 488 1
1.10.472.10 Gene3D IPR013763 Cyclin-like 75 176 13 Gene3D Transcription factor TFIIB 379 429 1
1.10.472.10 Gene3D IPR013763 Cyclin-like 177 267 13 Gene3D OsmC/Ohr family 3 42 7
SSF57783 SuperFamily 2 45 8
SSF47954 SuperFamily IPR013763 Cyclin-like 177 264 15
SSF47954 SuperFamily IPR013763 Cyclin-like 84 175 15
PR00685 PRINTS IPR000812 Transcription factor TFIIB 129 148 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 15 35 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 219 235 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 251 265 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.41 Da
Charge -2.50
Codon Adaptation Index 0.42
Isoelectric point 6.01
Molecular weight 55.80 kDa
Number of residues 492

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS315
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S315 PMID:21712547
O-phospho-L-threonine 701
present during mitotic M phaseT320
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T320 PMID:21712547
experimental evidence T344 PMID:24763107
O4'-phospho-L-tyrosine 88
present during mitotic M phaseY313
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence Y313 PMID:21712547
sumoylated lysine 173
Annotation ExtensionEvidenceResidueReference
IDA PMID:26404184
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for brf1 (SPBC13E7.10c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
278.92during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.93during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
0.64during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
gene structure updated PMID:21511999434
Taxonomic Conservation
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in archaea238

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC13E7.10c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
affinity captured bybdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
affinity captured byrpc25DNA-directed RNA polymerase III complex subunit Rpc25 Affinity Capture-WesternPMID:19910488
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
External References
Database Identifier Description
NBRP SPBC13E7.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC13E7.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC13E7.10c BioGRID Interaction Datasets
Expression Viewer SPBC13E7.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC13E7.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC13E7.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC13E7.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC13E7.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC13E7.10c Transcriptome Viewer (Bähler Lab)
GEO SPBC13E7.10c GEO profiles
PInt SPBC13E7.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC13E7.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC13E7.10c Fission yeast phenotypic data & analysis
Cyclebase SPBC13E7.10c.1 Cell Cycle Data
SPD / RIKEN40/40G02Orfeome Localization Data
UniProtKB/SwissProtQ9P6R0Transcription factor IIIB 60 kDa subunit
ModBaseQ9P6R0Database of comparative protein structure models
STRINGQ9P6R0Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596265transcription factor TFIIIB complex subunit Brf1

Literature for brf1

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016