brf1 (SPBC13E7.10c)


Gene Standard Namebrf1 Characterisation Statuspublished
Systematic IDSPBC13E7.10c Feature Typeprotein coding
SynonymsSPBC30D10.20 Name Description
Producttranscription factor TFIIIB complex subunit Brf1 Product Size492aa, 55.80 kDa
Genomic Location Chromosome II, 3058618-3056037 (2582nt); CDS:3058456-3056760 (1697nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0008270zinc ion bindingIEAIPR000812GO_REF:0000002249
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0045893positive regulation of transcription, DNA-templatedIEAIPR011665GO_REF:000000291
GO:0006384transcription initiation from RNA polymerase III promoterICGO:0000126PMID:1268236111
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005634nucleusIDAPMID:168233722737
GO:0000126transcription factor TFIIIB complexIDAPMID:126823613
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopybrf1ΔNullPMID:204732891337
Microscopybrf1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopybrf1ΔNullPECO:0000005, PECO:0000137PMID:23697806640
FYPO:0002262inviable after spore germination, multiple cell divisions, elongated cell95
penetrance FYPO_EXT:0000001Microscopybrf1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0000310inviable after spore germination, without cell division, with normal germ tube morphologyMicroscopybrf1ΔNullPECO:0000005, PECO:0000137PMID:23697806303
FYPO:0002151inviable sporeMicroscopybrf1ΔNullPECO:0000005, PECO:0000137PMID:23697806462
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
130586183058392
230583303058218
330580603056371
430562643056037
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08271 Pfam IPR013137 Zinc finger, TFIIB-type 1 42 2
PF07741 Pfam IPR011665 Brf1-like TBP-binding 391 487 1
PF00382 Pfam IPR013150 Transcription factor TFIIB, cyclin-like domain 183 256 2
PF00382 Pfam IPR013150 Transcription factor TFIIB, cyclin-like domain 89 156 2
SM00385 SMART IPR013763 Cyclin-like 181 265 13
SM00385 SMART IPR013763 Cyclin-like 87 168 13
PS51134 Prosite Profiles IPR013137 Zinc finger, TFIIB-type 1 30 2
PTHR11618:SF4 HMMPANTHER 1 492 1
PTHR11618 HMMPANTHER IPR000812 Transcription factor TFIIB 1 492 2
2.20.25.10 Gene3D 3 42 7
1.20.5.650 Gene3D 379 429 1
1.10.472.10 Gene3D IPR013763 Cyclin-like 75 176 13
1.10.472.10 Gene3D IPR013763 Cyclin-like 177 267 13
SSF47954 SuperFamily IPR013763 Cyclin-like 177 264 15
SSF57783 SuperFamily 2 45 8
SSF47954 SuperFamily IPR013763 Cyclin-like 84 175 15
PR00685 PRINTS IPR000812 Transcription factor TFIIB 219 235 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 251 265 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 15 35 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 129 148 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.41 Da
Charge -2.50
Isoelectric point 6.01
Molecular weight 55.80 kDa
Number of residues 492
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS315PMID:217125471670
present during mitotic M phase
MOD:00047O-phospho-L-threonineT320PMID:21712547690
present during mitotic M phase
T344PMID:24763107
MOD:00048O4'-phospho-L-tyrosineY313PMID:2171254784
present during mitotic M phase
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
278.92during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.93during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.64during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999432
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rpc25DNA-directed RNA polymerase III complex subunit Rpc25 Affinity Capture-WesternPMID:19910488
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
External References
Database Identifier Description
NBRP SPBC13E7.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC13E7.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC13E7.10c BioGRID Interaction Datasets
Expression Viewer SPBC13E7.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC13E7.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC13E7.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC13E7.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC13E7.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC13E7.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC13E7.10c Cell Cycle Data
GEO SPBC13E7.10c GEO profiles
PInt SPBC13E7.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC13E7.10c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN40/40G02Orfeome Localization Data
UniProtKB/SwissProtQ9P6R0Transcription factor IIIB 60 kDa subunit
ModBaseQ9P6R0Database of comparative protein structure models
STRINGQ9P6R0Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596265transcription factor TFIIIB complex subunit Brf1
European Nucleotide ArchiveCAB89885.2ENA Protein Mapping
UniParcUPI000228F4DFUniProt Archive

Literature for brf1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014