brf1 (SPBC13E7.10c)


Gene Standard Namebrf1 Characterisation Statuspublished
Systematic IDSPBC13E7.10c Feature Typeprotein coding
SynonymsSPBC30D10.20 Name Description
Producttranscription factor TFIIIB complex subunit Brf1 Product Size492aa, 55.80 kDa
Genomic Location Chromosome II, 3058618-3056037 (2582nt); CDS:3058456-3056760 (1697nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0008270zinc ion bindingIEAIPR000812GO_REF:0000002248
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0045893positive regulation of transcription, DNA-templatedIEAIPR011665GO_REF:000000298
GO:0006384transcription initiation from RNA polymerase III promoterTASPMID:1268236111
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005634nucleusIDAPMID:168233722730
GO:0000126transcription factor TFIIIB complexIDAPMID:126823613
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopybrf1ΔNullPECO:0000005, PECO:0000137PMID:236978061309
Microscopybrf1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopybrf1ΔNullPECO:0000005, PECO:0000137PMID:23697806564
FYPO:0002262inviable after spore germination, multiple cell divisions, elongated cellMicroscopybrf1ΔNullPECO:0000005, PECO:0000137PMID:2369780687
penetrance FYPO_EXT:0000001
FYPO:0000310inviable after spore germination, without cell division, with normal germ tube morphologyMicroscopybrf1ΔNullPECO:0000005, PECO:0000137PMID:23697806303
FYPO:0002151inviable sporeMicroscopybrf1ΔNullPECO:0000005, PECO:0000137PMID:23697806447
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
130586183058392
230583303058218
330580603056371
430562643056037

UTRs

Region Start End Reference
five_prime_UTR30586183058457PMID:21511999
three_prime_UTR30562643056037PMID:21511999
three_prime_UTR30567593056371PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08271 Pfam IPR013137 Zinc finger, TFIIB-type 1 42 2
PF00382 Pfam IPR013150 Transcription factor TFIIB, cyclin-like domain 183 256 2
PF00382 Pfam IPR013150 Transcription factor TFIIB, cyclin-like domain 89 156 2
PF07741 Pfam IPR011665 Brf1-like TBP-binding 391 487 1
SM00385 SMART IPR013763 Cyclin-like 181 265 13
SM00385 SMART IPR013763 Cyclin-like 87 168 13
PS51134 Prosite Profiles IPR013137 Zinc finger, TFIIB-type 1 30 2
PTHR11618:SF4 HMMPANTHER 1 492 1
PTHR11618 HMMPANTHER IPR000812 Transcription factor TFIIB 1 492 2
G3DSA:1.10.472.10 Gene3D IPR013763 177 267 13
G3DSA:1.10.472.10 Gene3D IPR013763 75 176 13
G3DSA:2.20.25.10 Gene3D 3 42 7
G3DSA:1.20.5.650 Gene3D 379 429 1
SSF57783 SuperFamily 2 45 8
SSF47954 SuperFamily IPR013763 177 264 15
SSF47954 SuperFamily IPR013763 84 175 15
Low complexity (SEG) seg 239 251
Low complexity (SEG) seg 381 407
Low complexity (SEG) seg 439 450
PR00685 PRINTS IPR000812 Transcription factor TFIIB 219 235 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 15 35 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 129 148 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 251 265 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.41 Da
Charge -2.50
Isoelectric point 6.01
Molecular weight 55.80 kDa
Number of residues 492
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
278.92during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.93during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.64during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999426
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rpc25DNA-directed RNA polymerase III complex subunit Rpc25 Affinity Capture-WesternPMID:19910488
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
External References
Database Identifier Description
NBRP SPBC13E7.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC13E7.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC13E7.10c BioGRID Interaction Datasets
Expression Viewer SPBC13E7.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC13E7.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC13E7.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC13E7.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC13E7.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC13E7.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC13E7.10c Cell Cycle Data
GEO SPBC13E7.10c GEO profiles
PInt SPBC13E7.10c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2540071transcription factor TFIIIB complex subunit Brf1
EntrezGene2540071transcription factor TFIIIB complex subunit Brf1
SPD / RIKEN40/40G02Orfeome Localization Data
UniProtKB/SwissProtQ9P6R0Transcription factor IIIB 60 kDa subunit
ModBaseQ9P6R0Database of comparative protein structure models
StringQ9P6R0Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596265transcription factor TFIIIB complex subunit Brf1
RefSeq mRNANM_001022185972h- transcription factor TFIIIB complex subunit Brf1 (brf1), mRNA
European Nucleotide ArchiveCAB89885ENA Protein Mapping
UniParcUPI000228F4DFUniProt Archive

Literature for brf1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014