myo1 (SPBC146.13c)


Gene Standard Namemyo1 Characterisation Statuspublished
Systematic IDSPBC146.13c Feature Typeprotein coding
Synonyms Name Description
Productmyosin type I Product Size1217aa, 135.71 kDa
Genomic Location Chromosome II, 1028192-1023963 (4230nt); CDS:1027930-1024233 (3698nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin binding38
Annotation ExtensionEvidenceWith/FromReference
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
microfilament motor activity4
Annotation ExtensionEvidenceWith/FromReference
phospholipid binding45
Annotation ExtensionEvidenceWith/FromReference
protein binding859
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin cortical patch assembly22
Annotation ExtensionEvidenceWith/FromReference
actin cortical patch localization16
Annotation ExtensionEvidenceWith/FromReference
actin cytoskeleton organization102
Annotation ExtensionEvidenceWith/FromReference
actin filament organization55
Annotation ExtensionEvidenceWith/FromReference
actin nucleation16
Annotation ExtensionEvidenceWith/FromReference
ascospore wall assembly13
Annotation ExtensionEvidenceWith/FromReference
endocytosis78
establishment or maintenance of cell polarity regulating cell shape56
Annotation ExtensionEvidenceWith/FromReference
plasma membrane raft distribution3
Annotation ExtensionEvidenceWith/FromReference
positive regulation of actin nucleation3
Annotation ExtensionEvidenceWith/FromReference
regulation of endocytosis14
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cortical patch47
Annotation ExtensionEvidenceWith/FromReference
actomyosin contractile ring38
Annotation ExtensionEvidenceWith/FromReference
cell tip194
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4209
Annotation ExtensionEvidenceWith/FromReference
medial cortex33
Annotation ExtensionEvidenceWith/FromReference
membrane1391
Annotation ExtensionEvidenceWith/FromReference
myosin I complex2
Annotation ExtensionEvidenceWith/FromReference
plasma membrane raft3
Annotation ExtensionEvidenceWith/FromReference
prospore membrane28
Annotation ExtensionEvidenceWith/FromReference
site of polarized growth41
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased mating efficiencymyo1ΔNull252
decreased vegetative cell population growthmyo1ΔNull571
increased number of cells with 1C DNA contentmyo1ΔNull40
normal growth on amphotericin Bmyo1ΔNull17
sensitive to 4-nitroquinoline N-oxidemyo1ΔNull159
sensitive to bleomycinmyo1ΔNull75
sensitive to cadmiummyo1ΔNull246
sensitive to caffeine during vegetative growthmyo1ΔNull106
sensitive to hydrogen peroxidemyo1ΔNull139
sensitive to hydroxyureamyo1ΔNull534
sensitive to micafunginmyo1ΔNull116
sensitive to salt stressmyo1ΔNull91
sensitive to tacrolimusmyo1ΔNull81
sensitive to thiabendazolemyo1ΔNull219
sensitive to UV during vegetative growthmyo1ΔNull142
viable vegetative cell populationmyo1ΔNull3809
unnamed (unrecorded)

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cycle arrest with unreplicated DNAmyo1ΔNull50
abnormal prospore formationmyo1ΔNull31
abnormal sporulation resulting in formation of ascus with more or fewer than four sporesmyo1ΔNull74
abolished protein localization to cell tip, with protein mislocalized to cytoplasm9
affecting syb1myo1ΔNull
decreased endocytosis during meiotic cell cyclemyo1ΔNull4
decreased endocytosis during vegetative growthmyo1ΔNull5
decreased protein localization to prospore membrane during meiosis II with protein mislocalized to plasma membrane2
affecting pmd1myo1ΔNull
affecting psy1myo1ΔNull
decreased sporulationmyo1ΔNull73
viable stubby vegetative cellmyo1ΔNull72
viable vegetative cell with abnormal cell shapemyo1ΔNull482
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in dip1 WISH/DIP/SPIN90 ortholog, endocytosis protein Dip1
GO localized by btn1 battenin CLN3 family protein
GO localized by dip1 WISH/DIP/SPIN90 ortholog, endocytosis protein Dip1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1028192..1027928, 1027883..1023963
mRNA1028192..1023963
5' UTR1028192..1027931PMID:21511999
CDS1027930..1027928, 1027883..1024233
3' UTR1024232..1023963PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00018 Pfam IPR001452 SH3 domain 1113 1157 18
PF00063 Pfam IPR001609 Myosin head, motor domain 42 707 5
PF06017 Pfam IPR010926 Myosin tail 2 765 963 1
SM00326 SMART IPR001452 SH3 domain 1109 1164 21
SM00242 SMART IPR001609 Myosin head, motor domain 34 721 5
PS50002 Prosite Profiles IPR001452 SH3 domain 1106 1165 21
PS51456 Prosite Profiles IPR001609 Myosin head, motor domain 40 720 5
PTHR13140:SF31 HMMPANTHER 38 791 1
PTHR13140 HMMPANTHER 38 791 6
2.30.30.40 Gene3D 1091 1133 21
2.30.30.40 Gene3D 1134 1173 21
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 124 163 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 36 764 294
SSF50044 SuperFamily IPR001452 SH3 domain 1096 1176 21
PR00193 PRINTS IPR001609 Myosin head, motor domain 173 200 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 70 89 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 462 490 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 409 437 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 126 151 5

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001026IQ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001026

Protein Properties

Ave. residue weight 111.51 Da
Charge 42.50
Isoelectric point 9.74
Molecular weight 135.71 kDa
Number of residues 1217
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
level fluctuates during mitotic cell cycleS1072
Annotation ExtensionEvidenceResidueReference
level fluctuates during mitotic cell cycle experimental evidence S1072 PMID:24763107
phosphorylated residue 1927
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
71341during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
76897during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
73687during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
80165during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
17856.67during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
76249during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
17375.55during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9.5during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesarp2ARP2/3 actin-organizing complex subunit Arp2 Affinity Capture-MSPMID:16087707
affinity capturesarp3Arp2/3 protein complex, actin-like protein subunit Arp3 Affinity Capture-MSPMID:16087707
affinity capturescam1calmodulin Cam1 Affinity Capture-WesternPMID:11260263
affinity capturescam2myosin I light chain Cam2 Affinity Capture-WesternPMID:15504913
binds activation domain construct withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Two-hybridPMID:12939254
forms complex withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Reconstituted ComplexPMID:12939254
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
affinity capturescsi1mitotic chromosome segregation protein Csi1 Affinity Capture-RNAPMID:19805578
affinity captured bycyk3cytokinesis protein Cyk3 (predicted) Affinity Capture-MSPMID:20603077
affinity capturesmcp7meiosis specific coiled-coil protein Mcp7 Affinity Capture-RNAPMID:19805578
affinity capturesmyo1myosin type I Affinity Capture-RNAPMID:19805578
affinity capturesmyo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
affinity captured bymyo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
affinity captured bymyo51myosin type V Affinity Capture-RNAPMID:19805578
affinity capturesmyo52myosin type V Affinity Capture-RNAPMID:19805578
affinity captured bymyo52myosin type V Affinity Capture-RNAPMID:19805578
affinity captured bymyp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
affinity capturesmyp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
affinity capturesrgf1RhoGEF for Rho1, Rgf1 Affinity Capture-RNAPMID:19805578
affinity capturesrng2IQGAP Affinity Capture-RNAPMID:19805578
affinity captured byrng3UCS-domain protein Rng3 Affinity Capture-RNAPMID:19098712
affinity capturesspp42U5 snRNP complex subunit Spp42 Affinity Capture-RNAPMID:19805578
affinity capturesuba5NEDD8 activating enzyme E1-type Uba5 (predicted) Affinity Capture-RNAPMID:19805578
affinity captured bywsp1WASp homolog Affinity Capture-RNAPMID:19805578
affinity captureswsp1WASp homolog Affinity Capture-RNAPMID:19805578
forms complex withwsp1WASp homolog Reconstituted ComplexPMID:12939254
binds DNA-binding domain construct withwsp1WASp homolog Two-hybridPMID:12939254
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withbzz1diacylglycerol binding protein Bzz1 (predicted) Synthetic LethalityPMID:21885283
synthetically rescuescdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic RescuePMID:21885283
synthetically rescued byeng2endo-1,3-beta-glucanase Eng2 Synthetic RescuePMID:25040903
synthetic lethal withubp9ubiquitin C-terminal hydrolase Ubp9 Synthetic LethalityPMID:20838651
synthetic growth defect withubp9ubiquitin C-terminal hydrolase Ubp9 Synthetic Growth DefectPMID:20838651
synthetic lethal withvrp1verprolin Synthetic LethalityPMID:16087707
synthetic lethal withwsp1WASp homolog Synthetic LethalityPMID:21885283
synthetic lethal withwsp1WASp homolog Synthetic LethalityPMID:11076964
External References
Database Identifier Description
NBRP SPBC146.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC146.13c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC146.13c BioGRID Interaction Datasets
Expression Viewer SPBC146.13c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC146.13c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC146.13c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC146.13c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC146.13c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC146.13c Transcriptome Viewer (Bähler Lab)
GEO SPBC146.13c GEO profiles
PInt SPBC146.13c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC146.13c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC146.13c Fission yeast phenotypic data & analysis
Cyclebase SPBC146.13c.1 Cell Cycle Data
SPD / RIKEN39/39C03Orfeome Localization Data
UniProtKB/SwissProtQ9Y7Z8Myosin-1
ModBaseQ9Y7Z8Database of comparative protein structure models
STRINGQ9Y7Z8Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595402myosin type I
RefSeq mRNANM_001021309972h- myosin type I (myo1), mRNA
European Nucleotide ArchiveCAB46766.1ENA Protein Mapping
UniParcUPI000006C1C9UniProt Archive

Literature for myo1

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015