myo1 (SPBC146.13c)


Gene Standard Namemyo1 Characterisation Statuspublished
Systematic IDSPBC146.13c Feature Typeprotein coding
Synonyms Name Description
Productmyosin type I Product Size1217aa, 135.71 kDa
Genomic Location Chromosome II, 1028192-1023963 (4230nt); CDS:1027930-1024233 (3698nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin binding38
Annotation ExtensionEvidenceWith/FromReference
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
microfilament motor activity4
Annotation ExtensionEvidenceWith/FromReference
phospholipid binding45
Annotation ExtensionEvidenceWith/FromReference
protein binding831
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin cortical patch assembly22
Annotation ExtensionEvidenceWith/FromReference
actin cortical patch localization16
Annotation ExtensionEvidenceWith/FromReference
actin cytoskeleton organization102
Annotation ExtensionEvidenceWith/FromReference
actin filament organization54
Annotation ExtensionEvidenceWith/FromReference
actin nucleation16
Annotation ExtensionEvidenceWith/FromReference
ascospore wall assembly14
Annotation ExtensionEvidenceWith/FromReference
endocytosis76
Annotation ExtensionEvidenceWith/FromReference
establishment or maintenance of cell polarity regulating cell shape58
Annotation ExtensionEvidenceWith/FromReference
plasma membrane raft distribution3
Annotation ExtensionEvidenceWith/FromReference
positive regulation of actin nucleation3
Annotation ExtensionEvidenceWith/FromReference
regulation of endocytosis14
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cortical patch47
Annotation ExtensionEvidenceWith/FromReference
actomyosin contractile ring30
Annotation ExtensionEvidenceWith/FromReference
cell tip190
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4205
Annotation ExtensionEvidenceWith/FromReference
medial cortex32
Annotation ExtensionEvidenceWith/FromReference
membrane1454
Annotation ExtensionEvidenceWith/FromReference
myosin I complex2
Annotation ExtensionEvidenceWith/FromReference
plasma membrane raft2
Annotation ExtensionEvidenceWith/FromReference
prospore membrane28
Annotation ExtensionEvidenceWith/FromReference
site of polarized growth37
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001355decreased vegetative cell population growthCell growth assaymyo1ΔNullPECO:0000005, PECO:0000200, PECO:0000142PMID:21850271381
FYPO:0001974increased number of cells with 1C DNA contentFlow cytometry datamyo1ΔNullPECO:0000170, PECO:0000137, PECO:0000005PMID:2317367238
FYPO:0000980normal growth on amphotericin BCell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:2373802117
FYPO:0001098sensitive to 4-nitroquinoline N-oxide159
expressivity FYPO_EXT:0000002Cell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:19264558
FYPO:0000095sensitive to bleomycinCell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:2317367248
FYPO:0000096sensitive to cadmium246
expressivity FYPO_EXT:0000001Cell growth assaymyo1ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
FYPO:0000097sensitive to caffeine105
expressivity FYPO_EXT:0000001Cell growth assaymyo1ΔNullPECO:0000137PMID:19672306
FYPO:0000087sensitive to hydrogen peroxide131
expressivity FYPO_EXT:0000001Cell growth assaymyo1ΔNullPECO:0000137PMID:19672306
FYPO:0000088sensitive to hydroxyureaCell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:23173672514
expressivity FYPO_EXT:0000003Cell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:19264558
FYPO:0002641sensitive to micafungin116
expressivity FYPO_EXT:0000002Cell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:23738021
FYPO:0000271sensitive to salt stress84
expressivity FYPO_EXT:0000003Cell growth assaymyo1ΔNullPECO:0000207, PECO:0000137, PECO:0000102, PECO:0000005PMID:18684775
FYPO:0000086sensitive to tacrolimus81
expressivity FYPO_EXT:0000001Cell growth assaymyo1ΔNullPECO:0000200, PECO:0000005, PECO:0000142PMID:21850271
FYPO:0000091sensitive to thiabendazoleCell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:23173672176
FYPO:0000268sensitive to UV during vegetative growthCell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:23173672123
FYPO:0002060viable vegetative cell populationMicroscopymyo1ΔNullPMID:204732893759
Microscopymyo1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopymyo1ΔNullPMID:20519959
Microscopynot recorded (unrecorded)PMID:11076964

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000196abnormal forespore formationMicroscopymyo1ΔNullPMID:2183215127
FYPO:0001430abnormal mitotic cell cycle arrest with unreplicated DNAFlow cytometry datamyo1ΔNullPECO:0000170, PECO:0000137, PECO:0000005PMID:2317367241
FYPO:0000421abolished endocytosis during vegetative growth6
affecting pmd1Microscopymyo1ΔNullPMID:21832151
affecting psy1Microscopymyo1ΔNullPMID:21832151
FYPO:0003209abolished protein localization to cell tip, with protein mislocalized to cytoplasm7
affecting syb1Microscopymyo1ΔNullPECO:0000126, PECO:0000005, PECO:0000142, PECO:0000106, PECO:0000225PMID:21850271
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidencemyo1ΔNullPECO:0000015PMID:23950735240
FYPO:0000584decreased sporulationMicroscopymyo1ΔNullPMID:2183215161
FYPO:0002106viable stubby vegetative cell69
penetrance FYPO_EXT:0000001Microscopymyo1ΔNullPECO:0000005, PECO:0000137PMID:23697806
expressivity FYPO_EXT:0000001
penetrance FYPO_EXT:0000002
experimental phenotypic evidencemyo1ΔNullPECO:0000137, PECO:0000005, PECO:0000103DOI:10.1016/j.devcel.2014.09.005
FYPO:0002197viable vegetative cell with abnormal cell shape467
penetrance FYPO_EXT:0000001experimental phenotypic evidencemyo1ΔNullPECO:0000005, PECO:0000103, PECO:0000137DOI:10.1016/j.devcel.2014.09.005
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in dip1 WISH/DIP/SPIN90 ortholog Dip1 PMID:21620704
GO localized by btn1 battenin CLN3 family protein PMID:18346214
GO localized by dip1 WISH/DIP/SPIN90 ortholog Dip1 PMID:21620704
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
110281921027928
210278831023963

UTRs

Region Coordinates Reference
five_prime_UTR1028192..1027931PMID:21511999
three_prime_UTR1024232..1023963PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00063 Pfam IPR001609 Myosin head, motor domain 42 707 5
PF06017 Pfam IPR010926 Myosin tail 2 765 963 1
PF00018 Pfam IPR001452 Src homology-3 domain 1113 1157 18
SM00242 SMART IPR001609 Myosin head, motor domain 34 721 5
SM00326 SMART IPR001452 Src homology-3 domain 1109 1164 21
PS50002 Prosite Profiles IPR001452 Src homology-3 domain 1106 1165 21
PTHR13140 HMMPANTHER 40 781 8
2.30.30.40 Gene3D 1091 1133 21
3.40.50.300 Gene3D 124 163 288
2.30.30.40 Gene3D 1134 1173 21
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 36 764 294
SSF50044 SuperFamily IPR001452 Src homology-3 domain 1096 1176 21
PR00193 PRINTS IPR001609 Myosin head, motor domain 173 200 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 70 89 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 462 490 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 409 437 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 126 151 5

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001026IQ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001026

Protein Properties

Ave. residue weight 111.51 Da
Charge 42.50
Isoelectric point 9.74
Molecular weight 135.71 kDa
Number of residues 1217
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS1072PMID:247631071670
level fluctuates during mitotic cell cycle
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
71341during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
76897during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
73687during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
80165during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
17856.67during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
76249during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
17375.55during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9.5during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
myo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
spp42U5 snRNP complex subunit Spp42 Affinity Capture-RNAPMID:19805578
cyk3cytokinesis protein Cyk3 (predicted) Affinity Capture-MSPMID:20603077
rng2IQGAP Affinity Capture-RNAPMID:19805578
arp2ARP2/3 actin-organizing complex subunit Arp2 Affinity Capture-MSPMID:16087707
arp3Arp2/3 protein complex, actin-like protein subunit Arp3 Affinity Capture-MSPMID:16087707
myo1myosin type I Affinity Capture-RNAPMID:19805578
wsp1WASp homolog Affinity Capture-RNAPMID:19805578
Reconstituted ComplexPMID:12939254
Two-hybrid
mcp7meiosis specific coiled-coil protein Mcp7 Affinity Capture-RNAPMID:19805578
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
myo51myosin type V Affinity Capture-RNAPMID:19805578
myp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
cam1calmodulin Cam1 Affinity Capture-WesternPMID:11260263
uba5NEDD8 activating enzyme (predicted) Affinity Capture-RNAPMID:19805578
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Two-hybridPMID:12939254
Reconstituted Complex
rgf1RhoGEF for Rho1, Rgf1 Affinity Capture-RNAPMID:19805578
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
cam2myosin I light chain Cam2 Affinity Capture-WesternPMID:15504913
csi1mitotic chromosome segregation protein Csi1 Affinity Capture-RNAPMID:19805578
myo52myosin type V Affinity Capture-RNAPMID:19805578
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
bzz1diacylglycerol binding protein Bzz1 (predicted) Synthetic LethalityPMID:21885283
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic RescuePMID:21885283
eng2endo-1,3-beta-glucanase Eng2 Synthetic RescuePMID:25040903
wsp1WASp homolog Synthetic LethalityPMID:21885283
Synthetic LethalityPMID:11076964
ubp9ubiquitin C-terminal hydrolase Ubp9 Synthetic Growth DefectPMID:20838651
Synthetic Lethality
vrp1verprolin Synthetic LethalityPMID:16087707
External References
Database Identifier Description
NBRP SPBC146.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC146.13c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC146.13c BioGRID Interaction Datasets
Expression Viewer SPBC146.13c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC146.13c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC146.13c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC146.13c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC146.13c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC146.13c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC146.13c Cell Cycle Data
GEO SPBC146.13c GEO profiles
PInt SPBC146.13c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC146.13c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC146.13c Fission yeast phenotypic data & analysis
SPD / RIKEN39/39C03Orfeome Localization Data
UniProtKB/SwissProtQ9Y7Z8Myosin-1
ModBaseQ9Y7Z8Database of comparative protein structure models
STRINGQ9Y7Z8Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595402myosin type I
RefSeq mRNANM_001021309972h- myosin type I (myo1), mRNA
European Nucleotide ArchiveCAB46766.1ENA Protein Mapping
UniParcUPI000006C1C9UniProt Archive

Literature for myo1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014