myo1 (SPBC146.13c)


Gene Standard Namemyo1 Characterisation Statuspublished
Systematic IDSPBC146.13c Feature Typeprotein coding
Synonyms Name Description
Productmyosin type I Product Size1217aa, 135.71 kDa
Genomic Location Chromosome II, 1028192-1023963 (4230nt); CDS:1027930-1024233 (3698nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0003779actin bindingIEAUniProtKB-KW:KW-0009GO_REF:000003738
GO:0000146microfilament motor activityISMPFAM:PF00063GO_REF:00000014
GO:0005543phospholipid bindingTASPMID:1605117945
GO:0005515protein bindingIPIcam1PMID:11260263861
IPIcdc15PMID:12939254
IPIwsp1PMID:12939254
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000147actin cortical patch assembly22
different_pathwayIGIvrp1PMID:16087707
GO:0051666actin cortical patch localizationIMPPMID:1126026314
GO:0030036actin cytoskeleton organizationIMPPMID:1107696496
GO:0007015actin filament organizationIMPPMID:1126026348
GO:0045010actin nucleationIDAPMID:1608770711
different_pathwayIGIvrp1PMID:16087707
GO:0030476ascospore wall assemblyIMPPMID:1126026315
GO:0006897endocytosisISOSGD:S000004715GO_REF:000002465
GO:0071963establishment or maintenance of cell polarity regulating cell shapeIMPPMID:1107696437
GO:0031580membrane raft distributionIMPPMID:160511793
GO:1902411mitotic barrier septum assemblyIMPPMID:1107696410
GO:0051127positive regulation of actin nucleationIMPPMID:110769643
GO:0030100regulation of endocytosisIMPPMID:1834621414
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030479actin cortical patchIDAPMID:1107696447
IDAPMID:21620704
GO:0005826actomyosin contractile ringTASPMID:1221423631
GO:0051286cell tipIDAPMID:16051179188
GO:0005737cytoplasmIDAPMID:168233724201
GO:0031097medial cortexIDAPMID:1605117933
GO:0016020membraneIMPPMID:160511791442
GO:0045121membrane raftIDAPMID:160511792
GO:0045160myosin I complexTASPMID:110769642
GO:0005628prospore membrane24
during meiosis IIIDAPMID:17202724
GO:0030427site of polarized growthIDAGO_REF:000000134
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001355decreased vegetative cell population growthCell growth assaymyo1ΔNullPECO:0000005, PECO:0000200, PECO:0000142PMID:21850271305
FYPO:0001974increased number of cells with 1C DNA contentFlow cytometry datamyo1ΔNullPECO:0000170, PECO:0000137, PECO:0000005PMID:2317367234
FYPO:0000980normal growth on amphotericin BCell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:2373802117
FYPO:0001098sensitive to 4-nitroquinoline N-oxide159
expressivity FYPO_EXT:0000002Cell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:19264558
FYPO:0000268sensitive to UV during vegetative growthCell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:23173672117
FYPO:0000095sensitive to bleomycinCell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:2317367247
FYPO:0000096sensitive to cadmium246
expressivity FYPO_EXT:0000001cell growth assaymyo1ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
FYPO:0000097sensitive to caffeine98
expressivity FYPO_EXT:0000001Cell growth assaymyo1ΔNullPECO:0000137PMID:19672306
FYPO:0000087sensitive to hydrogen peroxide121
expressivity FYPO_EXT:0000001Cell growth assaymyo1ΔNullPECO:0000137PMID:19672306
FYPO:0000088sensitive to hydroxyureaCell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:23173672506
expressivity FYPO_EXT:0000003Cell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:19264558
FYPO:0002641sensitive to micafungin108
expressivity FYPO_EXT:0000002Cell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:23738021
FYPO:0000271sensitive to salt stress82
expressivity FYPO_EXT:0000003cell growth assaymyo1ΔNullPECO:0000207, PECO:0000137, PECO:0000102, PECO:0000005PMID:18684775
FYPO:0000086sensitive to tacrolimus81
expressivity FYPO_EXT:0000001Cell growth assaymyo1ΔNullPECO:0000200, PECO:0000005, PECO:0000142PMID:21850271
FYPO:0000091sensitive to thiabendazoleCell growth assaymyo1ΔNullPECO:0000137, PECO:0000005PMID:23173672164
FYPO:0002060viable vegetative cell populationmyo1ΔNullPMID:205199593760
Microscopymyo1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopymyo1ΔNullPMID:20473289
not recorded (unrecorded)PMID:11076964

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001430abnormal mitotic cell cycle arrest with unreplicated DNAFlow cytometry datamyo1ΔNullPECO:0000170, PECO:0000137, PECO:0000005PMID:2317367237
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidencemyo1ΔNullPECO:0000015PMID:23950735230
FYPO:0002106viable stubby vegetative cell50
penetrance FYPO_EXT:0000001Microscopymyo1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in dip1 WISH/DIP/SPIN90 ortholog Dip1 PMID:21620704
GO localized by btn1 battenin CLN3 family protein PMID:18346214
GO localized by dip1 WISH/DIP/SPIN90 ortholog Dip1 PMID:21620704
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
110281921027928
210278831023963

UTRs

Region Coordinates Reference
five_prime_UTR1028192..1027931PMID:21511999
three_prime_UTR1024232..1023963PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00063 Pfam IPR001609 Myosin head, motor domain 42 707 5
PF00018 Pfam IPR001452 SH3 domain 1113 1157 18
PF06017 Pfam IPR010926 Myosin tail 2 765 963 1
SM00326 SMART IPR001452 SH3 domain 1109 1164 21
SM00242 SMART IPR001609 Myosin head, motor domain 34 721 5
PS50002 Prosite Profiles IPR001452 SH3 domain 1106 1165 21
PTHR13140 HMMPANTHER 38 791 6
PTHR13140:SF31 HMMPANTHER 38 791 1
2.30.30.40 Gene3D 1091 1133 21
2.30.30.40 Gene3D 1134 1173 21
SSF50044 SuperFamily IPR001452 SH3 domain 1096 1176 21
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 36 764 294
PR00193 PRINTS IPR001609 Myosin head, motor domain 409 437 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 126 151 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 462 490 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 70 89 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 173 200 5

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001026IQ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001026

Protein Properties

Ave. residue weight 111.51 Da
Charge 42.50
Isoelectric point 9.74
Molecular weight 135.71 kDa
Number of residues 1217
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS1072PMID:247631071663
level fluctuates during mitotic cell cycle
MOD:00696phosphorylated residueNASPMID:195477441915
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
17856.67during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
76249during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
76897during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
71341during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
17375.55during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
73687during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
80165during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
9.5during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
2.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
bzz1diacylglycerol binding protein Bzz1 (predicted) Synthetic LethalityPMID:21885283
ubp9ubiquitin C-terminal hydrolase Ubp9 Synthetic LethalityPMID:20838651
Synthetic Growth Defect
vrp1verprolin Synthetic LethalityPMID:16087707
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic RescuePMID:21885283
wsp1WASp homolog Synthetic LethalityPMID:21885283
Synthetic LethalityPMID:11076964
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
myo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
spp42U5 snRNP complex subunit Spp42 Affinity Capture-RNAPMID:19805578
cyk3cytokinesis protein Cyk3 (predicted) Affinity Capture-MSPMID:20603077
rng2IQGAP Affinity Capture-RNAPMID:19805578
arp2ARP2/3 actin-organizing complex subunit Arp2 Affinity Capture-MSPMID:16087707
arp3Arp2/3 protein complex, actin-like protein subunit Arp3 Affinity Capture-MSPMID:16087707
myo1myosin type I Affinity Capture-RNAPMID:19805578
wsp1WASp homolog Affinity Capture-RNAPMID:19805578
Reconstituted ComplexPMID:12939254
Two-hybrid
mcp7meiosis specific coiled-coil protein Mcp7 Affinity Capture-RNAPMID:19805578
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
myo51myosin type V Affinity Capture-RNAPMID:19805578
myp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
cam1calmodulin Cam1 Affinity Capture-WesternPMID:11260263
uba5NEDD8 activating enzyme (predicted) Affinity Capture-RNAPMID:19805578
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Two-hybridPMID:12939254
Reconstituted Complex
rgf1RhoGEF for Rho1, Rgf1 Affinity Capture-RNAPMID:19805578
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
cam2myosin I light chain Cam2 Affinity Capture-WesternPMID:15504913
csi1chromosome segregation protein Csi1 Affinity Capture-RNAPMID:19805578
myo52myosin type V Affinity Capture-RNAPMID:19805578
External References
Database Identifier Description
NBRP SPBC146.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC146.13c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC146.13c BioGRID Interaction Datasets
Expression Viewer SPBC146.13c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC146.13c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC146.13c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC146.13c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC146.13c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC146.13c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC146.13c Cell Cycle Data
GEO SPBC146.13c GEO profiles
PInt SPBC146.13c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2539659myosin type I
EntrezGene2539659myosin type I
SPD / RIKEN39/39C03Orfeome Localization Data
UniProtKB/SwissProtQ9Y7Z8Myosin-1
ModBaseQ9Y7Z8Database of comparative protein structure models
STRINGQ9Y7Z8Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595402myosin type I
RefSeq mRNANM_001021309972h- myosin type I (myo1), mRNA
European Nucleotide ArchiveCAB46766ENA Protein Mapping
European Nucleotide ArchiveCAB46766.1ENA Protein Mapping
UniParcUPI000006C1C9UniProt Archive

Literature for myo1

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014