myo1 (SPBC146.13c)


Gene Standard Namemyo1 Characterisation Statuspublished
Systematic IDSPBC146.13c Feature Typeprotein coding
Synonyms Name Description
Productmyosin type I Product Size1217aa, 135.71 kDa
Genomic Location Chromosome II, 1028192-1023963 (4230nt); CDS:1027930-1024233 (3698nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin binding37
Annotation ExtensionEvidenceWith/FromReference
ATP binding551
Annotation ExtensionEvidenceWith/FromReference
microfilament motor activity4
Annotation ExtensionEvidenceWith/FromReference
phospholipid binding47
Annotation ExtensionEvidenceWith/FromReference
protein binding900
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin cortical patch assembly21
Annotation ExtensionEvidenceWith/FromReference
actin cytoskeleton organization92
Annotation ExtensionEvidenceWith/FromReference
actin nucleation14
Annotation ExtensionEvidenceWith/FromReference
endocytosis74
establishment or maintenance of cell polarity regulating cell shape55
Annotation ExtensionEvidenceWith/FromReference
plasma membrane raft distribution1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of actin nucleation2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cortical patch46
Annotation ExtensionEvidenceWith/FromReference
actomyosin contractile ring38
Annotation ExtensionEvidenceWith/FromReference
cell cortex of cell tip49
Annotation ExtensionEvidenceWith/FromReference
cell division site318
Annotation ExtensionEvidenceWith/FromReference
cell tip211
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4212
Annotation ExtensionEvidenceWith/FromReference
mating projection tip29
Annotation ExtensionEvidenceWith/FromReference
medial cortex35
Annotation ExtensionEvidenceWith/FromReference
myosin I complex2
Annotation ExtensionEvidenceWith/FromReference
plasma membrane raft3
Annotation ExtensionEvidenceWith/FromReference
prospore membrane33
Annotation ExtensionEvidenceWith/FromReference
site of polarized growth59
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
abnormal mitotic cell cycle arrest with unreplicated DNAmyo1Δ58
decreased cell population growth at high temperaturemyo1Δ186
decreased cell population growth on glucose carbon sourcemyo1Δ393
decreased mating efficiencymyo1Δ276
decreased sporulation frequencymyo1Δ103
decreased vegetative cell population growthmyo1Δ745
increased number of cells with 1C DNA contentmyo1Δ66
increased vegetative cell population growth during phosphate starvationmyo1Δ3
S361A (S361A)
inviable vegetative cell populationS361A (S361A)1468
myo1head (head domain only)
loss of viability in stationary phasemyo1Δ73
normal growth on amphotericin Bmyo1Δ17
normal growth on sorbitolmyo1Δ21
normal vegetative cell population growthS361A (S361A)846
myo1head (head domain only)
S361D (S361D)
myo1-tail (tail domain only)
normal vegetative cell population growth during phosphate starvationS361D (S361D)3
sensitive to 4-nitroquinoline N-oxidemyo1Δ159
sensitive to bleomycinmyo1Δ93
sensitive to cadmiummyo1Δ321
sensitive to caffeine during vegetative growthmyo1Δ110
sensitive to hydrogen peroxidemyo1Δ160
sensitive to hydroxyureamyo1Δ595
sensitive to latrunculin Amyo1Δ39
sensitive to methyl methanesulfonatemyo1Δ333
sensitive to micafunginmyo1Δ119
sensitive to potassium chloridemyo1head (head domain only)30
myo1Δ
S361A (S361A)
sensitive to salt stressmyo1Δ102
sensitive to tacrolimusmyo1Δ81
sensitive to thiabendazolemyo1Δ334
sensitive to UV during vegetative growthmyo1Δ161
slow vegetative cell population growthS361A (S361A)369
myo1Δ
viable vegetative cell populationmyo1Δ3862

Cell Phenotype

Term NameGenotypesCount
abnormal actin cytoskeleton during meiosismyo1Δ1
abnormal cell wallmyo1Δ52
abnormal plasma membrane sterol distributionmyo1Δ7
abnormal prospore formationmyo1Δ38
abnormal sporulation resulting in formation of ascus with more or fewer than four sporesmyo1Δ103
abolished entry into G0myo1Δ1
abolished protein localization to cell tip, with protein mislocalized to cytoplasm10
affects localization of syb1myo1Δ
abolished sporulationmyo1Δ56
actin cortical patches present in increased numbersmyo1Δ1
decreased endocytosis during meiotic cell cyclemyo1Δ4
decreased endocytosis during vegetative growthmyo1Δ6
decreased level of stress responsive gene mRNA during cellular response to phosphate starvationmyo1Δ1
decreased protein localization to prospore membrane during meiosis II with protein mislocalized to plasma membrane2
affects localization of pmd1myo1Δ
affects localization of psy1myo1Δ
dispersed actin cortical patch localizationmyo1Δ34
viable elongated vegetative cell during phosphate starvationmyo1Δ1
viable stubby vegetative cellmyo1Δ76
viable vegetative cell with abnormal cell shapemyo1Δ709

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
abolished cell population growth at high temperatureubp9Δ, myo1Δ
sensitive to latrunculin Bubp9Δ, myo1Δ

Cell Phenotype

Term NameGenotypes
abnormal vegetative cell shapeubp9Δ, myo1Δ
decreased endocytosis during vegetative growthubp9Δ, myo1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO localization affected by mutation in btn1 battenin CLN3 family protein
FYPO localization affected by mutation in dip1 WISH/DIP/SPIN90 ortholog, endocytosis protein Dip1
GO localized by dip1 WISH/DIP/SPIN90 ortholog, endocytosis protein Dip1
GO substrate of sst2 human AMSH/STAMBP protein homolog, ubiquitin specific-protease
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
GO substrate of ubp9 ubiquitin C-terminal hydrolase Ubp9
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1028192..1027928, 1027883..1023963
Intron1027927..1027884
mRNA1028192..1023963
5' UTR1028192..1027931PMID:21511999
CDS1027930..1027928, 1027883..1024233
3' UTR1024232..1023963PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF06017 Pfam IPR010926 Class I myosin tail homology domain 765 958 1
PF00018 Pfam IPR001452 SH3 domain 1113 1157 20
PF00063 Pfam IPR001609 Myosin head, motor domain 42 707 5
SM00242 SMART IPR001609 Myosin head, motor domain 34 721 5
SM00326 SMART IPR001452 SH3 domain 1109 1164 21
PS51456 Prosite Profiles IPR001609 Myosin head, motor domain 40 720 5
PS51757 Prosite Profiles IPR010926 Class I myosin tail homology domain 778 964 1
PS50002 Prosite Profiles IPR001452 SH3 domain 1106 1165 21
PTHR13140:SF463 HMMPANTHER 774 794 1
PTHR13140:SF463 HMMPANTHER 32 753 1
PTHR13140 HMMPANTHER 32 753 7
PTHR13140 HMMPANTHER 774 794 7
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 124 163 288
2.30.30.40 Gene3D Phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma 1134 1173 21
2.30.30.40 Gene3D Phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma 1091 1133 21
SSF50044 SuperFamily IPR001452 SH3 domain 1096 1176 21
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 36 764 294
PR00193 PRINTS IPR001609 Myosin head, motor domain 173 200 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 462 490 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 126 151 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 70 89 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 409 437 5

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001026IQ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001026

Protein Properties

Ave. residue weight 111.51 Da
Charge 42.50
Codon Adaptation Index 0.50
Isoelectric point 9.74
Molecular weight 135.71 kDa
Number of residues 1217
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS361, S782, S1173, S1174, S1211 2293
level fluctuates during mitotic cell cycleS1072
Annotation ExtensionEvidenceResidueReference
IDA S1173 PMID:25720772
IDA S782 PMID:25720772
IDA S361 PMID:25720772
IDA S1211 PMID:25720772
IDA S1174 PMID:25720772
level fluctuates during mitotic cell cycle experimental evidence S1072 PMID:24763107
O-phospho-L-threonineT39 1087
Annotation ExtensionEvidenceResidueReference
IDA T39 PMID:25720772
O4'-phospho-L-tyrosineY363, Y781 281
Annotation ExtensionEvidenceResidueReference
IDA Y781 PMID:25720772
IDA Y363 PMID:25720772
phosphorylated residue 2515
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
O-phosphorylated residueS361,Y363 2461
Annotation ExtensionEvidenceResidueReference
IDA S361,Y363 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for myo1 (SPBC146.13c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
71341during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
76897during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
73687during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
80165during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
76249during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
17856.67during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
17375.55during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9.5during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
2.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4608
conserved in eukaryotes4516
conserved in metazoa3498
conserved in vertebrates3473
conserved in eukaryotes only2525
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC146.13c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byrng3UCS-domain protein Rng3 Affinity Capture-RNAPMID:19098712
affinity captured bymyo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
affinity captured bymyo51myosin type V Affinity Capture-RNAPMID:19805578
affinity captured bywsp1WASp homolog Affinity Capture-RNAPMID:19805578
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
affinity captured bycyk3Nebulin-family actin filament anchoring protein Cyk3 (predicted) Affinity Capture-MSPMID:20603077
affinity captured bymyo52myosin type V Affinity Capture-RNAPMID:19805578
affinity captured bymyp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
affinity capturescam2myosin I light chain Cam2 Affinity Capture-WesternPMID:15504913
affinity capturesrng2IQGAP Affinity Capture-RNAPMID:19805578
affinity capturescam1calmodulin Cam1 Affinity Capture-WesternPMID:11260263
affinity capturescsi1mitotic centromere-SPB clustering protein Csi1 Affinity Capture-RNAPMID:19805578
affinity capturesmcp7meiosis specific coiled-coil protein Mcp7 Affinity Capture-RNAPMID:19805578
affinity captureswsp1WASp homolog Affinity Capture-RNAPMID:19805578
affinity capturesrgf1RhoGEF for Rho1, Rgf1 Affinity Capture-RNAPMID:19805578
affinity capturesmyo52myosin type V Affinity Capture-RNAPMID:19805578
affinity capturesmyo1myosin type I Affinity Capture-RNAPMID:19805578
affinity capturesspp42U5 snRNP complex subunit Spp42 Affinity Capture-RNAPMID:19805578
affinity capturesarp2ARP2/3 actin-organizing complex subunit Arp2 Affinity Capture-MSPMID:16087707
affinity capturesarp3Arp2/3 protein complex, actin-like protein subunit Arp3 Affinity Capture-MSPMID:16087707
affinity capturesmyp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
affinity capturesmyo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
affinity capturesuba5NEDD8 activating enzyme E1-type Uba5 (predicted) Affinity Capture-RNAPMID:19805578
binds activation domain construct withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Two-hybridPMID:12939254
binds DNA-binding domain construct withwsp1WASp homolog Two-hybridPMID:12939254
forms complex withwsp1WASp homolog Reconstituted ComplexPMID:12939254
forms complex withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Reconstituted ComplexPMID:12939254
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC146.13c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withbzz1diacylglycerol binding protein Bzz1 (predicted) Synthetic LethalityPMID:21885283
synthetic lethal withvrp1verprolin Synthetic LethalityPMID:16087707
synthetic lethal withubp9ubiquitin C-terminal hydrolase Ubp9 Synthetic LethalityPMID:20838651
synthetic lethal withwsp1WASp homolog Synthetic LethalityPMID:21885283
synthetically rescued byeng2endo-1,3-beta-glucanase Eng2 Synthetic RescuePMID:25040903
synthetically rescuescdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic RescuePMID:21885283
synthetic growth defect withubp9ubiquitin C-terminal hydrolase Ubp9 Synthetic Growth DefectPMID:20838651
External References
Database Identifier Description
NBRP SPBC146.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC146.13c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC146.13c BioGRID Interaction Datasets
Expression Viewer SPBC146.13c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC146.13c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC146.13c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC146.13c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC146.13c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC146.13c Transcriptome Viewer (Bähler Lab)
GEO SPBC146.13c GEO profiles
PInt SPBC146.13c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC146.13c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC146.13c Fission yeast phenotypic data & analysis
Cyclebase SPBC146.13c.1 Cell Cycle Data
SPD / RIKEN39/39C03Orfeome Localization Data
UniProtKB/SwissProtQ9Y7Z8Myosin-1
ModBaseQ9Y7Z8Database of comparative protein structure models
STRINGQ9Y7Z8Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595402myosin type I
RefSeq mRNANM_001021309972h- myosin type I (myo1), mRNA
European Nucleotide ArchiveCAB46766.1ENA Protein Mapping
UniParcUPI000006C1C9UniProt Archive

Literature for myo1

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017