bot1 (SPBC14C8.16c)

Gene Standard Namebot1 Characterisation Statuspublished
Systematic IDSPBC14C8.16c Feature Typeprotein coding
Synonyms Name Description
Productmitochondrial ribosomal protein subunit S35 Product Size315aa, 35.75 kDa
Genomic Location Chromosome II, 2233275-2231539 (1737nt); CDS:2233111-2232164 (948nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
structural constituent of ribosome256
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitochondrial translation148
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
mitochondrial small ribosomal subunit32
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viability depends on conditions

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationbot1Δ1468
resistance to cell wall-degrading enzymesbot1+4
viable vegetative cell populationbot1Δ3862

Cell Phenotype

Term NameGenotypesCount
decreased cellular respirationbot1-GFP (GFP tagged bot1)9
decreased mitochondrial translationbot1-Myc (Myc tagged bot1)4
bot1-GFP (GFP tagged bot1)
decreased oxygen consumption during vegetative growthbot1-GFP (GFP tagged bot1)19
dispersed actin cortical patch localizationbot1+34
elongated vegetative cellbot1+810
fragmented mitochondriabot1+6
increased cellular respirationbot1+3
increased oxygen consumption during vegetative growthbot1+3
inviable after spore germination, multiple cell divisions, elongated tapered cellbot1Δ4
inviable elongated tapered vegetative cellbot1+3
normal actin cytoskeleton morphology during vegetative growthbot1+17
normal cellular mtDNA levelbot1+4
normal cytoplasmic microtubulesbot1+14
normal mitochondrial morphologybot1+5
viable vegetative cell with normal cell morphologybot1Δ3103
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR2233275..2233112PMID:21511999
3' UTR2232163..2231539PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF12298 Pfam IPR021036 Ribosomal protein S35, mitochondrial 117 278 1
PTHR28158 HMMPANTHER 1 312 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.50 Da
Charge 15.50
Codon Adaptation Index 0.42
Isoelectric point 10.19
Molecular weight 35.75 kDa
Number of residues 315
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for bot1 (SPBC14C8.16c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7991during GO:0000080PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
8797during GO:0000084PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
7750during GO:0000085PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
8474during GO:0000087PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
6535.71during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
8336during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.5during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
0.51during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
predominantly single copy (one to one)3087
conserved in fungi only552
conserved in fungi4608
conserved in eukaryotes4516

Manually curated orthologous groups


Orthologs in Compara

External References
Database Identifier Description
NBRP SPBC14C8.16c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC14C8.16c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC14C8.16c BioGRID Interaction Datasets
Expression Viewer SPBC14C8.16c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC14C8.16c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC14C8.16c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC14C8.16c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC14C8.16c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC14C8.16c Transcriptome Viewer (Bähler Lab)
GEO SPBC14C8.16c GEO profiles
PInt SPBC14C8.16c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC14C8.16c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC14C8.16c Fission yeast phenotypic data & analysis
Cyclebase SPBC14C8.16c.1 Cell Cycle Data
SPD / RIKEN13/13C12Orfeome Localization Data
UniProtKB/SwissProtO6009637S ribosomal protein S35, mitochondrial
ModBaseO60096Database of comparative protein structure models
STRINGO60096Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595919mitochondrial ribosomal protein subunit S35
RefSeq mRNANM_001021827972h- mitochondrial ribosomal protein subunit S35 (bot1), mRNA
European Nucleotide ArchiveAF352796ENA EMBL mapping
European Nucleotide ArchiveCAA18433.1ENA Protein Mapping
UniParcUPI000006C926UniProt Archive

Literature for bot1

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017