bot1 (SPBC14C8.16c)


Gene Standard Namebot1 Characterisation Statuspublished
Systematic IDSPBC14C8.16c Feature Typeprotein coding
Synonyms Name Description
Productmitochondrial ribosomal protein subunit S35 Product Size315aa, 35.75 kDa
Genomic Location Chromosome II, 2233275-2231539 (1737nt); CDS:2233111-2232164 (948nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
structural constituent of ribosome255
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
aerobic respiration41
Annotation ExtensionEvidenceWith/FromReference
mitochondrial translation153
Annotation ExtensionEvidenceWith/FromReference
mitochondrion distribution8
Annotation ExtensionEvidenceWith/FromReference
regulation of cell shape82
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
mitochondrial small ribosomal subunit32
Annotation ExtensionEvidenceWith/FromReference
mitochondrion758
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viability depends on conditions

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopybot1ΔNullPMID:204732891338
Microscopynot recorded (unrecorded)PMID:18245278
FYPO:0002060viable vegetative cell populationMicroscopybot1ΔNullPECO:0000005, PECO:0000137PMID:236978063759

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000014tapered cellbot1ΔNullPMID:18245278142
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopybot1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
122332752231539

UTRs

Region Coordinates Reference
five_prime_UTR2233275..2233112PMID:21511999
three_prime_UTR2232163..2231539PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF12298 Pfam IPR021036 Ribosomal protein S35, mitochondrial 117 276 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.50 Da
Charge 15.50
Isoelectric point 10.19
Molecular weight 35.75 kDa
Number of residues 315
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7991during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
8797during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
7750during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
8474during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
8336during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
6535.71during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.5during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.51during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only623
conserved in fungi4600
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPBC14C8.16c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC14C8.16c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC14C8.16c BioGRID Interaction Datasets
Expression Viewer SPBC14C8.16c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC14C8.16c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC14C8.16c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC14C8.16c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC14C8.16c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC14C8.16c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC14C8.16c Cell Cycle Data
GEO SPBC14C8.16c GEO profiles
PInt SPBC14C8.16c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC14C8.16c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC14C8.16c Fission yeast phenotypic data & analysis
SPD / RIKEN13/13C12Orfeome Localization Data
UniProtKB/SwissProtO6009637S ribosomal protein S35, mitochondrial
ModBaseO60096Database of comparative protein structure models
STRINGO60096Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595919mitochondrial ribosomal protein subunit S35
RefSeq mRNANM_001021827972h- mitochondrial ribosomal protein subunit S35 (bot1), mRNA
European Nucleotide ArchiveAF352796ENA EMBL mapping
European Nucleotide ArchiveCAA18433.1ENA Protein Mapping
UniParcUPI000006C926UniProt Archive

Literature for bot1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014