tts1 (SPBC1539.04)


Gene Standard Nametts1 Characterisation Statuspublished
Systematic IDSPBC1539.04 Feature Typeprotein coding
Synonyms Name DescriptionTeTra Spanning protein
Producttetra spanning protein 1, Tts1 Product Size279aa, 31.57 kDa
Genomic Location Chromosome II, 4368286-4369816 (1531nt); CDS:4368389-4369414 (1026nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding854
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

mitotic cytokinesis


Term NameCount
cellular protein localization553
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum tubular network organization3
Annotation ExtensionEvidenceWith/FromReference
establishment of spindle pole body localization to nuclear envelope3
Annotation ExtensionEvidenceWith/FromReference
mitotic cytokinesis, site selection8
Annotation ExtensionEvidenceWith/FromReference
nuclear envelope organization13
Annotation ExtensionEvidenceWith/FromReference
spindle pole body localization to nuclear envelope3
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site305
Annotation ExtensionEvidenceWith/FromReference
cortical endoplasmic reticulum5
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum tubular network5
Annotation ExtensionEvidenceWith/FromReference
Golgi apparatus357
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
nuclear envelope134
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
viable vegetative cell populationtts1ΔNull3792

Cell Phenotype

Term NameAlleleExpressionCount
abnormal cytokinesisunnamed (unrecorded)225
abnormal mitotic spindle assemblytts1ΔNull12
abnormal nuclear pore distribution during mitosis2FD (F248D, F255D)Not specified2
2RI (R208I, R210I)Not specified
tts1ΔNull
decreased protein localization to endoplasmic reticulum tubular network1
affecting tts12RI (R208I, R210I)Not specified
affecting tts12TM-Cter (83-193)Not specified
affecting tts14TM (194-279)Not specified
Δ-H1 (196-212)Not specified
normal nuclear envelope morphology during mitotic interphasetts1ΔNull1
normal nuclear pore distribution during mitotic interphasetts1ΔNull1
normal protein localization to mitotic spindle pole body13
affecting cut11tts1ΔNull
nuclear envelope protrusion present during mitosistts1ΔNull5
viable vegetative cell with normal cell morphologytts1ΔNull3092
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in tts1 tetra spanning protein 1, Tts1 PMID:25103238
GO localized by rtn1 reticulon-like protein Rtn1 PMID:20434336
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4368286..4368448, 4368635..4369816
mRNA4368286..4369816
5' UTR4368286..4368388PMID:21511999
CDS4368389..4368448, 4368635..4369414
3' UTR4369415..4369816PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03661 Pfam IPR005344 Uncharacterised protein family UPF0121 25 256 1
TMhelix TMHMM 100 122 959
TMhelix TMHMM 25 47 959
TMhelix TMHMM 170 192 959
TMhelix TMHMM 57 79 959
PTHR12703 HMMPANTHER 2 267 1
PTHR12703:SF4 HMMPANTHER 2 267 1

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0000418signal_peptide279
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 113.15 Da
Charge 25.00
Isoelectric point 11.09
Molecular weight 31.57 kDa
Number of residues 279
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased during GO:0071470expression microarray evidencePMID:12529438
increased during GO:0034605expression microarray evidencePMID:12529438
increased during GO:0034599expression microarray evidencePMID:12529438
increased in presence of cadmium sulfate expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
12057during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
12617during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
11542during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
11394during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6577.81during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
10809during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
7921.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9.3during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
co-purifies withrtn1reticulon-like protein Rtn1 Co-purificationPMID:20434336
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withcut11spindle pole body docking protein Cut11 Synthetic LethalityPMID:25103238
overexpression rescuescut11spindle pole body docking protein Cut11 Dosage RescuePMID:25103238
enhances phenotype ofcut11spindle pole body docking protein Cut11 Phenotypic EnhancementPMID:25103238
phenotype enhanced byrtn1reticulon-like protein Rtn1 Phenotypic EnhancementPMID:20434336
rescued byscs2VAP family protein Scs2 Phenotypic SuppressionPMID:23041194
rescued byscs22VAP family protein Scs22 Phenotypic SuppressionPMID:23041194
phenotype enhanced byyop1ER membrane protein DP1/Yop1 Phenotypic EnhancementPMID:20434336
External References
Database Identifier Description
NBRP SPBC1539.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1539.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1539.04 BioGRID Interaction Datasets
Expression Viewer SPBC1539.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1539.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1539.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1539.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1539.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1539.04 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1539.04 Cell Cycle Data
GEO SPBC1539.04 GEO profiles
PInt SPBC1539.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1539.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1539.04 Fission yeast phenotypic data & analysis
SPD / RIKEN15/15D12Orfeome Localization Data
UniProtKB/SwissProtQ9Y7Z5Tetra-spanning protein 1
ModBaseQ9Y7Z5Database of comparative protein structure models
STRINGQ9Y7Z5Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596818tetra spanning protein 1, Tts1
RefSeq mRNANM_001023838972h- tetra spanning protein 1, Tts1 (tts1), mRNA
European Nucleotide ArchiveCAB51336.1ENA Protein Mapping
UniParcUPI000006AA70UniProt Archive

Literature for tts1

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015