tts1 (SPBC1539.04)


Gene Standard Nametts1 Characterisation Statuspublished
Systematic IDSPBC1539.04 Feature Typeprotein coding
Synonyms Name DescriptionTeTra Spanning protein
Producttetra spanning protein 1, Tts1 Product Size279aa, 31.57 kDa
Genomic Location Chromosome II, 4368286-4369816 (1531nt); CDS:4368389-4369414 (1026nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding863
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular protein localization508
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum tubular network organization4
Annotation ExtensionEvidenceWith/FromReference
establishment of spindle pole body localization to nuclear envelope3
Annotation ExtensionEvidenceWith/FromReference
mitotic cytokinesis, site selection7
Annotation ExtensionEvidenceWith/FromReference
nuclear envelope organization12
Annotation ExtensionEvidenceWith/FromReference
spindle pole body localization to nuclear envelope3
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site313
Annotation ExtensionEvidenceWith/FromReference
cortical endoplasmic reticulum5
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum tubular network5
Annotation ExtensionEvidenceWith/FromReference
Golgi apparatus357
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane963
Annotation ExtensionEvidenceWith/FromReference
nuclear envelope129
Annotation ExtensionEvidenceWith/FromReference
nuclear membrane33
Annotation ExtensionEvidenceWith/FromReference
nuclear pore51
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationtts1Δ3837

Cell Phenotype

Term NameGenotypesCount
abnormal cytokinesis326
abnormal mitotic spindle assemblytts1Δ17
abnormal nuclear pore distribution during mitosistts1Δ3
2FD (F248D, F255D)
2RI (R208I, R210I)
decreased protein localization to endoplasmic reticulum tubular networkΔ-H1 (196-212)1
affects localization of tts14TM (194-279)
affects localization of tts12RI (R208I, R210I)
affects localization of tts12TM-Cter (83-193)
normal nuclear envelope morphology during mitotic interphasetts1Δ1
normal nuclear pore distribution during mitotic interphasetts1Δ1
normal protein localization to mitotic spindle pole body20
affecting cut11tts1Δ
nuclear envelope protrusion present during mitosistts1Δ8
viable vegetative cell with normal cell morphologytts1Δ3100

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
abolished cell population growth at high temperaturecut11-6 (T498I), tts1Δ
inviable vegetative cell populationcut11-6 (T498I), tts1Δ
viable vegetative cell populationcut11-6 (T498I), tts1Δ
tts1+, cut11-6 (T498I)

Cell Phenotype

Term NameGenotypes
abnormal establishment of mitotic spindle pole body localization to nuclear envelopecut11-6 (T498I), tts1Δ
abnormal septum site selectionrtn1Δ, Δ-H1 (196-212), yop1Δ
rtn1Δ, yop1Δ, 2RI (R208I, R210I)
rtn1Δ, yop1Δ, tts1Δ
rtn1Δ, yop1Δ, 4TM (194-279)
2TM-Cter (83-193), rtn1Δ, yop1Δ
abolished protein localization to medial cortex
affects localization of mid2yop1Δ, rtn1Δ, tts1Δ
decreased protein localization to mitotic spindle pole body
affects localization of alp4cut11-6 (T498I), tts1Δ
affects localization of alp6cut11-6 (T498I), tts1Δ
delayed onset of mitotic spindle assemblycut11-6 (T498I), tts1Δ
mislocalized, misoriented septumrtn1Δ, yop1Δ, 2RI (R208I, R210I)
rtn1Δ, yop1Δ, 4TM (194-279)
rtn1Δ, Δ-H1 (196-212), yop1Δ
rtn1Δ, yop1Δ, tts1Δ
2TM-Cter (83-193), rtn1Δ, yop1Δ
misoriented septum during vegetative growthrtn1Δ, Δ-H1 (196-212), yop1Δ
rtn1Δ, yop1Δ, 2RI (R208I, R210I)
rtn1Δ, yop1Δ, 4TM (194-279)
rtn1Δ, yop1Δ, tts1Δ
2TM-Cter (83-193), rtn1Δ, yop1Δ
mitotic spindle absent from cellcut11-6 (T498I), tts1Δ
multiseptate vegetative cellrtn1Δ, yop1Δ, 4TM (194-279)
rtn1Δ, Δ-H1 (196-212), yop1Δ
2TM-Cter (83-193), rtn1Δ, yop1Δ
rtn1Δ, yop1Δ, tts1Δ
rtn1Δ, yop1Δ, 2RI (R208I, R210I)
normal protein localization to medial cortex during vegetative growth
affecting mid2scs2Δ, scs22Δ, tts1Δ, rtn1Δ, yop1Δ
normal septum orientationscs2Δ, scs22Δ, tts1Δ, rtn1Δ, yop1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO localization affected by mutation in tts1 tetra spanning protein 1, Tts1
GO localized by rtn1 reticulon-like protein Rtn1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4368286..4368448, 4368635..4369816
Intron4368449..4368634
5' UTR4368286..4368388PMID:21511999
3' UTR4369415..4369816PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03661 Pfam IPR005344 Per33/Pom33 family 19 256 1
TMhelix TMHMM 170 192 953
TMhelix TMHMM 100 122 953
TMhelix TMHMM 57 79 953
TMhelix TMHMM 25 47 953
PTHR12703 HMMPANTHER 2 258 1
PTHR12703:SF4 HMMPANTHER 2 258 1

View domain organization at Pfam

Term IDTerm NameReferenceCount
SO:0000418signal_peptide279
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 113.15 Da
Charge 25.00
Codon Adaptation Index 0.50
Isoelectric point 11.09
Molecular weight 31.57 kDa
Number of residues 279
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased during GO:0034599expression microarray evidencePMID:12529438
increased in presence of cadmium sulfate expression microarray evidencePMID:12529438
increased during GO:0071470expression microarray evidencePMID:12529438
increased during GO:0034605expression microarray evidencePMID:12529438

Quantitative Gene Expression

View graphical display of gene expression data for tts1 (SPBC1539.04)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
12057during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
12617during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
11542during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
11394during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
10809during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
6577.81during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
7921.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9.3during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC1539.04 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
co-purifies withrtn1reticulon-like protein Rtn1 Co-purificationPMID:20434336
binds activation domain construct withubp1ubiquitin C-terminal hydrolase Ubp1 Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC1539.04 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescued byscs2VAP family protein Scs2 Phenotypic SuppressionPMID:23041194
rescued byscs22VAP family protein Scs22 Phenotypic SuppressionPMID:23041194
overexpression rescuescut11spindle pole body docking protein Cut11 Dosage RescuePMID:25103238
phenotype enhanced byyop1ER membrane protein DP1/Yop1 Phenotypic EnhancementPMID:20434336
phenotype enhanced byrtn1reticulon-like protein Rtn1 Phenotypic EnhancementPMID:20434336
enhances phenotype ofcut11spindle pole body docking protein Cut11 Phenotypic EnhancementPMID:25103238
synthetic lethal withcut11spindle pole body docking protein Cut11 Synthetic LethalityPMID:25103238
External References
Database Identifier Description
NBRP SPBC1539.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1539.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1539.04 BioGRID Interaction Datasets
Expression Viewer SPBC1539.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1539.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1539.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1539.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1539.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1539.04 Transcriptome Viewer (Bähler Lab)
GEO SPBC1539.04 GEO profiles
PInt SPBC1539.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1539.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1539.04 Fission yeast phenotypic data & analysis
Cyclebase SPBC1539.04.1 Cell Cycle Data
SPD / RIKEN15/15D12Orfeome Localization Data
UniProtKB/SwissProtQ9Y7Z5Tetra-spanning protein 1
ModBaseQ9Y7Z5Database of comparative protein structure models
STRINGQ9Y7Z5Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596818tetra spanning protein 1, Tts1
RefSeq mRNANM_001023838972h- tetra spanning protein 1, Tts1 (tts1), mRNA

Literature for tts1

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016