gpt2 (SPBC15D4.04)

Gene Standard Namegpt2 Characterisation Statuspublished
Systematic IDSPBC15D4.04 Feature Typeprotein coding
Synonymsalg7, gpt Name Description
ProductUDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase Product Size446aa, 49.86 kDa
Genomic Location Chromosome II, 3018106-3019993 (1888nt); CDS:3018244-3019584 (1341nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
dolichyl diphosphate biosynthetic process1
Annotation ExtensionEvidenceWith/FromReference
N-glycan processing7
Annotation ExtensionEvidenceWith/FromReference
protein N-linked glycosylation32
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum595
Annotation ExtensionEvidenceWith/FromReference
integral component of endoplasmic reticulum membrane68
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane968
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationgpt2Δ1444
resistance to tunicamycingpt2+1

Cell Phenotype

Term NameGenotypesCount
increased UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activitygpt2+1
inviable after spore germination with normal, unseptated germ tube morphologygpt2Δ237
inviable sporegpt2Δ474
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR3018106..3018243PMID:21511999
3' UTR3019585..3019993PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00953 Pfam IPR000715 Glycosyl transferase, family 4 128 303 1
TMhelix TMHMM 309 331 959
TMhelix TMHMM 251 273 959
TMhelix TMHMM 192 209 959
TMhelix TMHMM 72 94 959
TMhelix TMHMM 414 436 959
TMhelix TMHMM 282 304 959
TMhelix TMHMM 124 143 959
TMhelix TMHMM 214 236 959
TMhelix TMHMM 155 177 959
PTHR10571:SF0 HMMPANTHER 1 446 1
PTHR10571 HMMPANTHER 1 446 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.78 Da
Charge 13.00
Isoelectric point 8.92
Molecular weight 49.86 kDa
Number of residues 446
Gene Expression

Qualitative Gene Expression

RNA levelpresent during GO:0072690Northern assay evidencePMID:7893167

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
18753during GO:0000080PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
21179during GO:0000084PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
18334during GO:0000085PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
21690during GO:0000087PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
3377.63during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
18793during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.6during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
0.23during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
functionally complemented by Chinese Hamster Ovary (CHO) L-G1PTfull PMID:7893167
functionally complemented by hamster Dpagt1full PMID:9455919
Taxonomic Conservation
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3421
conserved in vertebrates3396

Manually curated orthologous groups


Orthologs in Compara

External References
Database Identifier Description
NBRP SPBC15D4.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC15D4.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC15D4.04 BioGRID Interaction Datasets
Expression Viewer SPBC15D4.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC15D4.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC15D4.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC15D4.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC15D4.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC15D4.04 Transcriptome Viewer (Bähler Lab)
GEO SPBC15D4.04 GEO profiles
PInt SPBC15D4.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC15D4.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC15D4.04 Fission yeast phenotypic data & analysis
Cyclebase SPBC15D4.04.1 Cell Cycle Data
IntEnz2.7.8.15Integrated relational Enzyme database
Rhea2.7.8.15Annotated reactions database
SPD / RIKEN32/32H04Orfeome Localization Data
UniProtKB/SwissProtP42881UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
ModBaseP42881Database of comparative protein structure models
STRINGP42881Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596244UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
RefSeq mRNANM_001022163972h- UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (gpt2), mRNA
European Nucleotide ArchiveCU329671ENA EMBL mapping
European Nucleotide ArchiveU09454ENA EMBL mapping
European Nucleotide ArchiveAAA92799ENA Protein Mapping
European Nucleotide ArchiveAAA92799.1ENA Protein Mapping
European Nucleotide ArchiveCAA20479ENA Protein Mapping
European Nucleotide ArchiveCAA20479.1ENA Protein Mapping
MetaCycPWY-5265Peptidoglycan biosynthesis II (staphylococci)
MetaCycPWY-6470Peptidoglycan biosynthesis V (beta-lactam resistance)
MetaCycPWY-6471Peptidoglycan biosynthesis IV (Enterococcus faecium)
KEGG00550+ biosynthesis
UniParcUPI000012BA41UniProt Archive
UniPathwayUPA00219Cell wall biogenesis; peptidoglycan biosynthesis

Literature for gpt2

Search: Europe PMC or PubMed

Release Version: PomBase:29_56 - 12 Nov 2015