gpt2 (SPBC15D4.04)


Gene Standard Namegpt2 Characterisation Statuspublished
Systematic IDSPBC15D4.04 Feature Typeprotein coding
Synonymsalg7, gpt Name Description
ProductUDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase Product Size446aa, 49.86 kDa
Genomic Location Chromosome II, 3018106-3019993 (1888nt); CDS:3018244-3019584 (1341nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003975UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activityIMPPMID:94559191
heterologous_systemIGISGD:S000000447PMID:7893167
GO:0008963phospho-N-acetylmuramoyl-pentapeptide-transferase activityIEAIPR000715GO_REF:00000021
GO:0016757transferase activity, transferring glycosyl groupsIEAUniProtKB-KW:KW-0328GO_REF:000003793
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006491N-glycan processingIMPPMID:78931677
GO:0006489dolichyl diphosphate biosynthetic processIMPPMID:78931671
GO:0006487protein N-linked glycosylation31
heterologous_systemIGISGD:S000000447PMID:7893167
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005783endoplasmic reticulumIDAPMID:16823372593
heterologous_systemIGISGD:S000000447PMID:7893167
GO:0030176integral component of endoplasmic reticulum membraneICGO:0005783, GO:0016021GO_REF:000000167
GO:0016021integral component of membraneEXPPMID:78931671017
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopygpt2ΔNullPMID:204732891331
Cell growth assaygpt2ΔNullPMID:9455919
Microscopygpt2ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0001034resistance to tunicamycinCell growth assaygpt2+ (wild type)OverexpressionPMID:94559191
Cell growth assaynot recorded (wild type)OverexpressionPECO:0000103, PECO:0000126, PECO:0000005PMID:7893167
Cell growth assaynot recorded (wild type)OverexpressionPECO:0000102, PECO:0000126, PECO:0000005PMID:7893167

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003148increased UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activityEnzyme assay datagpt2+ (wild type)OverexpressionPMID:94559191
FYPO:0000311inviable after spore germination with normal, unseptated germ tube morphology237
penetrance FYPO_EXT:0000003Microscopygpt2ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0002151inviable spore459
penetrance FYPO_EXT:0000001Microscopygpt2ΔNullPECO:0000005, PECO:0000137PMID:23697806
Cell growth assaygpt2ΔNullPMID:7893167
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
130181063019993

UTRs

Region Coordinates Reference
five_prime_UTR3018106..3018243PMID:21511999
three_prime_UTR3019585..3019993PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00953 Pfam IPR000715 Glycosyl transferase, family 4 128 304 1
TMhelix TMHMM 192 209 959
TMhelix TMHMM 124 143 959
TMhelix TMHMM 251 273 959
TMhelix TMHMM 282 304 959
TMhelix TMHMM 414 436 959
TMhelix TMHMM 155 177 959
TMhelix TMHMM 72 94 959
TMhelix TMHMM 214 236 959
TMhelix TMHMM 309 331 959
PTHR10571 HMMPANTHER 1 446 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.78 Da
Charge 13.00
Isoelectric point 8.92
Molecular weight 49.86 kDa
Number of residues 446
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0072690Northern assay evidencePMID:7893167

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
18753during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
21179during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
18334during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
21690during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
3377.63during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
18793during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.6during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.23during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by Chinese Hamster Ovary (CHO) L-G1PTfull PMID:7893167
functionally complemented by hamster Dpagt1full PMID:9455919
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPBC15D4.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC15D4.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC15D4.04 BioGRID Interaction Datasets
Expression Viewer SPBC15D4.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC15D4.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC15D4.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC15D4.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC15D4.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC15D4.04 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC15D4.04 Cell Cycle Data
GEO SPBC15D4.04 GEO profiles
PInt SPBC15D4.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC15D4.04 Peptides identified in tandem mass spectrometry proteomics experiments
IntEnz2.7.8.15Integrated relational Enzyme database
Rhea2.7.8.15Annotated reactions database
EntrezGene2539795UDP-N-acetylglucosamine--dolichyl-phosphateN-acetylglucosaminephosphotransferase
WikiGene2539795UDP-N-acetylglucosamine--dolichyl-phosphateN-acetylglucosaminephosphotransferase
SPD / RIKEN32/32H04Orfeome Localization Data
UniProtKB/SwissProtP42881UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
ModBaseP42881Database of comparative protein structure models
STRINGP42881Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596244UDP-N-acetylglucosamine--dolichyl-phosphateN- acetylglucosaminephosphotransferase
RefSeq mRNANM_001022163972h- UDP-N-acetylglucosamine--dolichyl-phosphateN-acetylglucosaminephosphotransferase (gpt2), mRNA
European Nucleotide ArchiveAAA92799ENA Protein Mapping
European Nucleotide ArchiveAAA92799.1ENA Protein Mapping
European Nucleotide ArchiveCAA20479ENA Protein Mapping
European Nucleotide ArchiveCAA20479.1ENA Protein Mapping
UniParcUPI000012BA41UniProt Archive

Literature for gpt2

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014