gpt2 (SPBC15D4.04)


Gene Standard Namegpt2 Characterisation Statuspublished
Systematic IDSPBC15D4.04 Feature Typeprotein coding
Synonymsalg7, gpt Name Description
ProductUDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase Product Size446aa, 49.86 kDa
Genomic Location Chromosome II, 3018106-3019993 (1888nt); CDS:3018244-3019584 (1341nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003975UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity1
heterologous_systemIMPPMID:9455919
heterologous_systemIGISGD:S000000447PMID:7893167
GO:0008963phospho-N-acetylmuramoyl-pentapeptide-transferase activityIEAIPR000715GO_REF:00000021
GO:0016757transferase activity, transferring glycosyl groupsIEAUniProtKB-KW:KW-0328GO_REF:000003793
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006491N-glycan processingIMPPMID:78931673
GO:0006489dolichyl diphosphate biosynthetic processIMPPMID:78931671
GO:0006487protein N-linked glycosylation29
heterologous_systemIGISGD:S000000447PMID:7893167
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005783endoplasmic reticulumIDAPMID:16823372594
heterologous_systemIGISGD:S000000447PMID:7893167
GO:0030176integral component of endoplasmic reticulum membraneICGO:0005783, GO:0016021GO_REF:000000167
GO:0016021integral component of membraneEXPPMID:78931671016
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopygpt2ΔNullPECO:0000005, PECO:0000137PMID:236978061309
Microscopygpt2ΔNullPMID:20473289
FYPO:0001034resistance to tunicamycinCell growth assaynot recorded (wild type)OverexpressionPECO:0000103, PECO:0000126, PECO:0000005PMID:78931671
Cell growth assaynot recorded (wild type)OverexpressionPECO:0000102, PECO:0000126, PECO:0000005PMID:7893167

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000306abolished spore germinationCell growth assaygpt2ΔNullPMID:789316715
FYPO:0000311inviable after spore germination with normal, unseptated germ tube morphologyMicroscopygpt2ΔNullPECO:0000005, PECO:0000137PMID:23697806237
penetrance FYPO_EXT:0000003
FYPO:0002151inviable sporeMicroscopygpt2ΔNullPECO:0000005, PECO:0000137PMID:23697806447
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
130181063019993

UTRs

Region Start End Reference
five_prime_UTR30181063018243PMID:21511999
three_prime_UTR30195853019993PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00953 Pfam IPR000715 Glycosyl transferase, family 4 128 304 1
TMhelix TMHMM 192 209 959
TMhelix TMHMM 124 143 959
TMhelix TMHMM 282 304 959
TMhelix TMHMM 251 273 959
TMhelix TMHMM 155 177 959
TMhelix TMHMM 414 436 959
TMhelix TMHMM 72 94 959
TMhelix TMHMM 214 236 959
TMhelix TMHMM 309 331 959
PTHR10571:SF0 HMMPANTHER 1 446 1
PTHR10571 HMMPANTHER 1 446 1
Low complexity (SEG) seg 123 146
Low complexity (SEG) seg 167 180

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.78 Da
Charge 13.00
Isoelectric point 8.92
Molecular weight 49.86 kDa
Number of residues 446
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresentNorthern assay evidencePMID:7893167
during GO:0072690

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
3377.63during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.23during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
1.6during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReferenceCount
functionally complemented by Chinese Hamster Ovary (CHO) L-G1PTfull PMID:78931671
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPBC15D4.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC15D4.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC15D4.04 BioGRID Interaction Datasets
Expression Viewer SPBC15D4.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC15D4.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC15D4.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC15D4.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC15D4.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC15D4.04 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC15D4.04 Cell Cycle Data
GEO SPBC15D4.04 GEO profiles
PInt SPBC15D4.04 Protein-Protein Interaction Predictor (Bähler Lab)
IntEnz2.7.8.15Integrated relational Enzyme database
Rhea2.7.8.15Annotated reactions database
EntrezGene2539795UDP-N-acetylglucosamine--dolichyl-phosphateN-acetylglucosaminephosphotransferase
WikiGene2539795UDP-N-acetylglucosamine--dolichyl-phosphateN-acetylglucosaminephosphotransferase
SPD / RIKEN32/32H04Orfeome Localization Data
UniProtKB/SwissProtP42881UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
ModBaseP42881Database of comparative protein structure models
StringP42881Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596244UDP-N-acetylglucosamine--dolichyl-phosphateN- acetylglucosaminephosphotransferase
RefSeq mRNANM_001022163972h- UDP-N-acetylglucosamine--dolichyl-phosphateN-acetylglucosaminephosphotransferase (gpt2), mRNA
European Nucleotide ArchiveAAA92799ENA Protein Mapping
European Nucleotide ArchiveCAA20479ENA Protein Mapping
UniParcUPI000012BA41UniProt Archive

Literature for gpt2

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014