gpt2 (SPBC15D4.04)


Gene Standard Namegpt2 Characterisation Statuspublished
Systematic IDSPBC15D4.04 Feature Typeprotein coding
Synonymsalg7, gpt Name Description
ProductUDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase Product Size446aa, 49.86 kDa
Genomic Location Chromosome II, 3018106-3019993 (1888nt); CDS:3018244-3019584 (1341nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
dolichyl diphosphate biosynthetic process1
Annotation ExtensionEvidenceWith/FromReference
N-glycan processing7
Annotation ExtensionEvidenceWith/FromReference
protein N-linked glycosylation32
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
integral component of endoplasmic reticulum membrane68
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationgpt2ΔNull1428
resistance to tunicamycingpt2+ (wild type)Overexpression1

Cell Phenotype

Term NameAlleleExpressionCount
increased UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activitygpt2+ (wild type)Overexpression1
inviable after spore germination with normal, unseptated germ tube morphologygpt2ΔNull237
inviable sporegpt2ΔNull468
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3018106..3019993
mRNA3018106..3019993
5' UTR3018106..3018243PMID:21511999
CDS3018244..3019584
3' UTR3019585..3019993PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00953 Pfam IPR000715 Glycosyl transferase, family 4 128 304 1
TMhelix TMHMM 251 273 959
TMhelix TMHMM 309 331 959
TMhelix TMHMM 124 143 959
TMhelix TMHMM 414 436 959
TMhelix TMHMM 282 304 959
TMhelix TMHMM 214 236 959
TMhelix TMHMM 192 209 959
TMhelix TMHMM 155 177 959
TMhelix TMHMM 72 94 959
PTHR10571:SF0 HMMPANTHER 1 446 1
PTHR10571 HMMPANTHER 1 446 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.78 Da
Charge 13.00
Isoelectric point 8.92
Molecular weight 49.86 kDa
Number of residues 446
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0072690Northern assay evidencePMID:7893167

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
18753during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
21179during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
18334during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
21690during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
18793during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3377.63during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.6during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.23during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by Chinese Hamster Ovary (CHO) L-G1PTfull PMID:7893167
functionally complemented by hamster Dpagt1full PMID:9455919
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPBC15D4.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC15D4.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC15D4.04 BioGRID Interaction Datasets
Expression Viewer SPBC15D4.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC15D4.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC15D4.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC15D4.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC15D4.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC15D4.04 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC15D4.04 Cell Cycle Data
GEO SPBC15D4.04 GEO profiles
PInt SPBC15D4.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC15D4.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC15D4.04 Fission yeast phenotypic data & analysis
IntEnz2.7.8.15Integrated relational Enzyme database
Rhea2.7.8.15Annotated reactions database
SPD / RIKEN32/32H04Orfeome Localization Data
UniProtKB/SwissProtP42881UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
ModBaseP42881Database of comparative protein structure models
STRINGP42881Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596244UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
RefSeq mRNANM_001022163972h- UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (gpt2), mRNA
European Nucleotide ArchiveAAA92799.1ENA Protein Mapping
European Nucleotide ArchiveCAA20479.1ENA Protein Mapping
MetaCycPWY-5265Peptidoglycan biosynthesis II (staphylococci)
MetaCycPWY-6470Peptidoglycan biosynthesis V (beta-lactam resistance)
MetaCycPWY-6471Peptidoglycan biosynthesis IV (Enterococcus faecium)
KEGG_Enzyme00550+2.7.8.13Peptidoglycan biosynthesis
UniParcUPI000012BA41UniProt Archive
UniPathwayUPA00219Cell wall biogenesis; peptidoglycan biosynthesis

Literature for gpt2

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015