amo1 (SPBC15D4.10c)


Gene Standard Nameamo1 Characterisation Statuspublished
Systematic IDSPBC15D4.10c Feature Typeprotein coding
Synonyms Name DescriptionAberrent Microtubules when Overexpressed
Productnuclear rim protein Amo1 Product Size475aa, 51.79 kDa
Genomic Location Chromosome II, 3030224-3028685 (1540nt); CDS:3030192-3028705 (1488nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037748
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0007049cell cycleIEAUniProtKB-KW:KW-0131GO_REF:0000037631
GO:0031122cytoplasmic microtubule organizationIMPPMID:1579792512
GO:0040023establishment of nucleus localizationIMPPMID:1579792521
GO:0071963establishment or maintenance of cell polarity regulating cell shapeIGItea1PMID:1579792527
GO:0030951establishment or maintenance of microtubule cytoskeleton polarityIMPPMID:157979253
GO:0031115negative regulation of microtubule polymerization1
requiredIMPPMID:15797925
during mitotic interphase
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0031965nuclear membraneIDAPMID:1579792551
GO:0005634nucleusIDAPMID:168233722740
NOT GO:0044732mitotic spindle pole bodyIDAPMID:15797925
NOT GO:0005643nuclear poreIDAPMID:15797925
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002766resistance to clotrimazole10
expressivity FYPO_EXT:0000003Cell growth assayamo1ΔNullPECO:0000137, PECO:0000005PMID:22252817
FYPO:0002634resistance to cobalt56
expressivity FYPO_EXT:0000002Cell growth assayamo1ΔNullPECO:0000201, PECO:0000005PMID:22806344
FYPO:0002767resistance to terbinafine11
expressivity FYPO_EXT:0000003Cell growth assayamo1ΔNullPECO:0000137, PECO:0000005PMID:22252817
FYPO:0002642sensitive to amphotericin BCell growth assayamo1ΔNullPECO:0000137, PECO:0000005PMID:2225281769
FYPO:0000088sensitive to hydroxyurea511
expressivity FYPO_EXT:0000003Cell Growth Assayamo1ΔNullPECO:0000137, PECO:0000102PMID:23697806
FYPO:0002060viable vegetative cell populationMicroscopyamo1ΔNullPECO:0000005, PECO:0000137PMID:236978063730
Microscopyamo1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000016curved vegetative cellMicroscopynot recorded (unrecorded)PMID:1579792599
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidenceamo1ΔNullPECO:0000015PMID:23950735235
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopyamo1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
130302243028938
230288773028685

UTRs

Region Coordinates Reference
three_prime_UTR3028704..3028685PMID:18641648
three_prime_UTR3028704..3028685PMID:20118936
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PS50103 Prosite Profiles IPR000571 Zinc finger, CCCH-type 1 25 14
PTHR21495 HMMPANTHER 1 326 1
PTHR21495:SF39 HMMPANTHER 1 326 1
Coil ncoils Rabaptin coiled-coil domain 111 132 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000137zinc finger proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000137
PBO:0000416zf-CCCH typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000416

Protein Properties

Ave. residue weight 109.04 Da
Charge 4.00
Isoelectric point 8.59
Molecular weight 51.79 kDa
Number of residues 475
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS142PMID:217125471669
present during mitotic M phase
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
3434during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
3597during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
2190during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
NDduring GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.6during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog579
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
hip1hira protein, histone chaperone Hip1 Negative GeneticPMID:18818364
pph3serine/threonine protein phosphatase, PP4 complex subunit Pph3 (predicted) Negative GeneticPMID:18818364
klp6kinesin-like protein Klp6 Positive GeneticPMID:18818364
elp5elongator complex subunit Elp5 (predicted) Negative GeneticPMID:18818364
kap114karyopherin Kap14 Positive GeneticPMID:18818364
hht2histone H3 h3.2 Negative GeneticPMID:18818364
swd1Set1C complex subunit Swd1 Positive GeneticPMID:18818364
elp3elongator complex subunit Elp3 (predicted) Negative GeneticPMID:18818364
rem1meiosis-specific cyclin Rem1 Negative GeneticPMID:18818364
pmk1MAP kinase Pmk1 Positive GeneticPMID:18818364
bag101BAG family molecular chaperone regulator Bag101 (predicted) Negative GeneticPMID:18818364
ers1RNA-silencing factor Ers1 Positive GeneticPMID:18818364
syj2inositol-polyphosphate 5-phosphatase, synaptojanin homolog 2 (predicted) Negative GeneticPMID:18818364
rad57RecA family ATPase Rad57/Rhp57 Negative GeneticPMID:18818364
rhp7Rad7 homolog Rhp7 Negative GeneticPMID:18818364
swc5Swr1 complex subunit Swc5 Positive GeneticPMID:18818364
SPAC3A11.03methyltransferase (predicted) Negative GeneticPMID:18818364
ccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:18818364
obr1ubiquitinated histone-like protein Uhp1 Positive GeneticPMID:18818364
SPBP8B7.10cU3 snoRNP-associated protein Utp16 (predicted) Negative GeneticPMID:18818364
hip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
ebs1EST1 family nonsense-mediated mRNA decay (NMD) pathway protein Ebs1 (predicted) Negative GeneticPMID:18818364
fkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Negative GeneticPMID:18818364
eaf1RNA polymerase II transcription elongation factor SpEAF Positive GeneticPMID:18818364
nap1nucleosome assembly protein Nap1 Positive GeneticPMID:18818364
rhp26SNF2 family helicase Rhp26 Negative GeneticPMID:18818364
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:18818364
sum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:18818364
lub1WD repeat protein Lub1 Negative GeneticPMID:18818364
nup131nucleoporin Nup131 Negative GeneticPMID:18818364
utp4U3 snoRNP protein Utp4 (predicted) Negative GeneticPMID:18818364
mal3EB1 family Mal3 Negative GeneticPMID:18818364
mre11Mre11 nuclease Negative GeneticPMID:18818364
rsv2transcription factor Rsv2 Negative GeneticPMID:18818364
SPBC16C6.05mitochondrial translation initiation factor (predicted) Negative GeneticPMID:18818364
rad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Negative GeneticPMID:18818364
tma20RNA-binding protein Tma20 (predicted) Negative GeneticPMID:18818364
cdk11serine/threonine protein kinase cdk11 Negative GeneticPMID:18818364
SPBC29A3.09cAAA family ATPase Gcn20 (predicted) Negative GeneticPMID:18818364
ies2Ino80 complex subunit Ies2 Positive GeneticPMID:18818364
SPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:18818364
sgf29SAGA complex subunit Sgf29 Positive GeneticPMID:18818364
kap113karyopherin Kap113 Positive GeneticPMID:18818364
but1neddylation pathway protein But1 Positive GeneticPMID:18818364
pht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:18818364
hrp3ATP-dependent DNA helicase Hrp3 Positive GeneticPMID:18818364
SPAC227.14uridine kinase (predicted) Negative GeneticPMID:18818364
srb11cyclin CycC, Srb mediator subunit Srb11 Negative GeneticPMID:18818364
rev7DNA polymerase zeta Rev7 (predicted) Negative GeneticPMID:18818364
hrp1ATP-dependent DNA helicase Hrp1 Negative GeneticPMID:18818364
SPCC645.13transcription elongation regulator/ ubiquitin-protein ligase (predicted) Negative GeneticPMID:18818364
bcp1thioredoxin peroxidase Bcp1 Positive GeneticPMID:18818364
sdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:18818364
hta1histone H2A alpha Positive GeneticPMID:18818364
clr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:18818364
SPBC16G5.03ubiquitin-protein ligase E3 (predicted) Negative GeneticPMID:18818364
rpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:18818364
png2ING family homolog Png2 Negative GeneticPMID:18818364
cho1phosphatidyl-N-methylethanolamine N-methyltransferase (predicted) Negative GeneticPMID:18818364
SPBC839.03cneddylation protein Dcn1 (predicted) Negative GeneticPMID:18818364
trm13tRNA 2'-O-methyltransferase Trm13 (predicted) Positive GeneticPMID:18818364
nup60nucleoporin Nup60 Negative GeneticPMID:18818364
rox3mediator complex subunit Med19/Rox3 Negative GeneticPMID:18818364
epe1Jmjc domain chromatin associated protein Epe1 Positive GeneticPMID:18818364
set5histone lysine methyltransferase Set5 (predicted) Negative GeneticPMID:18818364
rpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:18818364
mod21gamma tubulin complex subunit Mod21 Positive GeneticPMID:18818364
ptc2protein phosphatase 2C Ptc2 Negative GeneticPMID:18818364
chp1chromodomain protein Chp1 Negative GeneticPMID:18818364
ptf1Mst2 histone acetytransferase acytyltransferase complex, predicted phosphoric monoester hydrolase Ptf1 Negative GeneticPMID:18818364
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:18818364
ubp3ubiquitin C-terminal hydrolase Ubp3 Positive GeneticPMID:18818364
rhn1RNA polymerase II transcription termination factor homolog Negative GeneticPMID:18818364
reb1RNA polymerase I transcription termination factor Reb1 Negative GeneticPMID:18818364
pdx1pyruvate dehydrogenase protein x component, Pdx1 (predicted) Negative GeneticPMID:18818364
SPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:18818364
ppk8serine/threonine protein kinase Ppk8 (predicted) Negative GeneticPMID:18818364
SPAC323.03csequence orphan Negative GeneticPMID:18818364
ppk16serine/threonine protein kinase Ppk16 (predicted) Negative GeneticPMID:18818364
dad1DASH complex subunit Dad1 Negative GeneticPMID:18818364
pmp1dual-specificity MAP kinase phosphatase Pmp1 Negative GeneticPMID:18818364
SPBP8B7.23ubiquitin-protein ligase E3 implicated in transcription (predicted) Negative GeneticPMID:18818364
spn1septin Spn1 Negative GeneticPMID:18818364
cid12poly(A) polymerase Cid12 Positive GeneticPMID:18818364
cwf21complexed with Cdc5 protein Cwf21 Positive GeneticPMID:18818364
rcd1RNA-binding protein, CCR4-NOT complex subunit Rcd1 Negative GeneticPMID:18818364
bub3mitotic spindle checkpoint protein Bub3 Positive GeneticPMID:18818364
set9histone lysine H3-K20 methyltransferase Set9 Negative GeneticPMID:18818364
mms19Dos2 silencing complex subunit Mms19 Positive GeneticPMID:18818364
tea2kinesin-like protein Tea2 Negative GeneticPMID:18818364
klp3kinesin-like protein Klp3 Positive GeneticPMID:18818364
nup40nucleoporin Nup40 Negative GeneticPMID:18818364
dsk1SR protein-specific kinase Dsk1 Positive GeneticPMID:18818364
ppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:18818364
sdc1Dpy-30 domain protein Sdc1 Negative GeneticPMID:18818364
SPAC23A1.07ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:18818364
SPCC18.10pyridoxine-pyridoxal-pyridoxamine kinase (predicted) Negative GeneticPMID:18818364
ura5orotate phosphoribosyltransferase Ura5 Negative GeneticPMID:18818364
ssu72phosphoric ester hydrolase Ssu72 (predicted) Negative GeneticPMID:18818364
dcr1dicer Positive GeneticPMID:18818364
tfs1transcription elongation factor TFIIS Positive GeneticPMID:18818364
mlo2ubiquitin protein ligase E3 component human N-recognin 7 homolog Mlo2 Positive GeneticPMID:18818364
sir2Sir2 family histone deacetylase Sir2 Negative GeneticPMID:18818364
gnr1heterotrimeric G protein beta subunit Gnr1 Positive GeneticPMID:18818364
ufd2ubiquitin-protein ligase E4 (predicted) Negative GeneticPMID:18818364
External References
Database Identifier Description
NBRP SPBC15D4.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC15D4.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC15D4.10c BioGRID Interaction Datasets
Expression Viewer SPBC15D4.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC15D4.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC15D4.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC15D4.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC15D4.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC15D4.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC15D4.10c Cell Cycle Data
GEO SPBC15D4.10c GEO profiles
PInt SPBC15D4.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC15D4.10c Peptides identified in tandem mass spectrometry proteomics experiments
EntrezGene2539911nuclear rim protein Amo1
WikiGene2539911nuclear rim protein Amo1
SPD / RIKEN24/24A05Orfeome Localization Data
UniProtKB/SwissProtO74315Nucleoporin-like protein amo1
ModBaseO74315Database of comparative protein structure models
STRINGO74315Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596250nuclear rim protein Amo1
RefSeq mRNANM_001022169972h- nuclear rim protein Amo1 (amo1), mRNA
European Nucleotide ArchiveCAA20485ENA Protein Mapping
European Nucleotide ArchiveCAA20485.1ENA Protein Mapping
UniParcUPI000006A222UniProt Archive

Literature for amo1

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014