amo1 (SPBC15D4.10c)


Gene Standard Nameamo1 Characterisation Statuspublished
Systematic IDSPBC15D4.10c Feature Typeprotein coding
Synonyms Name DescriptionAberrent Microtubules when Overexpressed
Productnuclear rim protein Amo1 Product Size475aa, 51.79 kDa
Genomic Location Chromosome II, 3030224-3028685 (1540nt); CDS:3030192-3028705 (1488nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
metal ion binding747
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic microtubule organization11
Annotation ExtensionEvidenceWith/FromReference
establishment of nucleus localization20
Annotation ExtensionEvidenceWith/FromReference
establishment or maintenance of cell polarity regulating cell shape57
Annotation ExtensionEvidenceWith/FromReference
establishment or maintenance of microtubule cytoskeleton polarity3
Annotation ExtensionEvidenceWith/FromReference
negative regulation of microtubule polymerization1
Annotation ExtensionEvidenceWith/FromReference
during mitotic interphase
GO Cellular Component
Term NameCount
nuclear membrane33
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
NOT mitotic spindle pole body
Annotation ExtensionEvidenceWith/FromReference
NOT nuclear pore
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased mating efficiencyamo1ΔNull249
inviable vegetative cell populationamo1+ (wild type)Overexpression1428
resistance to clotrimazoleamo1ΔNull10
resistance to cobaltamo1ΔNull56
resistance to terbinafineamo1ΔNull11
sensitive to amphotericin Bamo1ΔNull69
sensitive to hydroxyureaamo1ΔNull525
viable vegetative cell populationamo1ΔNull3792

Cell Phenotype

Term NameAlleleExpressionCount
curved vegetative cellunnamed (unrecorded)107
viable vegetative cell with normal cell morphologyamo1ΔNull3092
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3030224..3028938, 3028877..3028685
mRNA3030224..3028685
5' UTR3030224..3030193AU013326
CDS3030192..3028938, 3028877..3028705
3' UTR3028704..3028685PMID:18641648
3' UTR3028704..3028685PMID:18641648
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PS50103 Prosite Profiles IPR000571 Zinc finger, CCCH-type 1 25 14
PTHR21495:SF39 HMMPANTHER 1 326 1
PTHR21495 HMMPANTHER 1 326 1
Coil ncoils Rabaptin coiled-coil domain 111 132 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000137zinc finger proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000137
PBO:0000416zf-CCCH typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000416

Protein Properties

Ave. residue weight 109.04 Da
Charge 4.00
Isoelectric point 8.59
Molecular weight 51.79 kDa
Number of residues 475
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS142
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S142 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3434during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3597during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2190during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.6during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog598
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withbag101BAG family molecular chaperone regulator Bag101 (predicted) Negative GeneticPMID:18818364
positive genetic interaction withbcp1thioredoxin peroxidase Bcp1 Positive GeneticPMID:18818364
positive genetic interaction withbub3mitotic spindle checkpoint protein Bub3 Positive GeneticPMID:18818364
positive genetic interaction withbut1neddylation pathway protein But1 Positive GeneticPMID:18818364
negative genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withcdk11serine/threonine protein kinase cdk11 Negative GeneticPMID:18818364
negative genetic interaction withcho1phosphatidyl-N-methylethanolamine N-methyltransferase (predicted) Negative GeneticPMID:18818364
negative genetic interaction withchp1chromodomain protein Chp1 Negative GeneticPMID:18818364
positive genetic interaction withcid12poly(A) polymerase Cid12 Positive GeneticPMID:18818364
negative genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:18818364
positive genetic interaction withcwf21complexed with Cdc5 protein Cwf21 Positive GeneticPMID:18818364
negative genetic interaction withdad1DASH complex subunit Dad1 Negative GeneticPMID:18818364
positive genetic interaction withdcr1dicer Positive GeneticPMID:18818364
positive genetic interaction withdsk1SR protein-specific kinase Dsk1 Positive GeneticPMID:18818364
positive genetic interaction witheaf1RNA polymerase II transcription elongation factor SpEAF Positive GeneticPMID:18818364
negative genetic interaction withebs1EST1 family nonsense-mediated mRNA decay (NMD) pathway protein Ebs1 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withelp3elongator complex subunit Elp3 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withelp5elongator complex subunit Elp5 (predicted) Negative GeneticPMID:18818364
positive genetic interaction withepe1Jmjc domain chromatin associated protein Epe1 Positive GeneticPMID:18818364
positive genetic interaction withers1RNA-silencing factor Ers1 Positive GeneticPMID:18818364
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:18818364
negative genetic interaction withfkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Negative GeneticPMID:18818364
negative genetic interaction withgcn20AAA family ATPase Gcn20 (predicted) Negative GeneticPMID:18818364
positive genetic interaction withgnr1heterotrimeric G protein beta subunit Gnr1 Positive GeneticPMID:18818364
negative genetic interaction withhht2histone H3 h3.2 Negative GeneticPMID:18818364
negative genetic interaction withhip1hira protein, histone chaperone Hip1 Negative GeneticPMID:18818364
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
negative genetic interaction withhrp1ATP-dependent DNA helicase Hrp1 Negative GeneticPMID:18818364
positive genetic interaction withhrp3ATP-dependent DNA helicase Hrp3 Positive GeneticPMID:18818364
positive genetic interaction withhta1histone H2A alpha Positive GeneticPMID:18818364
positive genetic interaction withies2Ino80 complex subunit Ies2 Positive GeneticPMID:18818364
positive genetic interaction withkap113karyopherin Kap113 Positive GeneticPMID:18818364
positive genetic interaction withkap114karyopherin Kap14 Positive GeneticPMID:18818364
positive genetic interaction withklp3kinesin-like protein Klp3 Positive GeneticPMID:18818364
positive genetic interaction withklp6kinesin-like protein Klp6 Positive GeneticPMID:18818364
negative genetic interaction withlub1WD repeat protein Lub1 Negative GeneticPMID:18818364
negative genetic interaction withmal3EB1 family Mal3 Negative GeneticPMID:18818364
positive genetic interaction withmlo2ubiquitin protein ligase E3 component human N-recognin 7 homolog Mlo2 Positive GeneticPMID:18818364
positive genetic interaction withmms19Dos2 silencing complex subunit Mms19 Positive GeneticPMID:18818364
positive genetic interaction withmod21gamma tubulin complex subunit Mod21 Positive GeneticPMID:18818364
negative genetic interaction withmre11Mre11 nuclease Negative GeneticPMID:18818364
positive genetic interaction withnap1nucleosome assembly protein Nap1 Positive GeneticPMID:18818364
negative genetic interaction withngg1SAGA complex subunit Ngg1 Negative GeneticPMID:18818364
negative genetic interaction withnup131nucleoporin Nup131 Negative GeneticPMID:18818364
negative genetic interaction withnup40nucleoporin Nup40 Negative GeneticPMID:18818364
negative genetic interaction withnup60nucleoporin Nup60 Negative GeneticPMID:18818364
positive genetic interaction withobr1ubiquitinated histone-like protein Uhp1 Positive GeneticPMID:18818364
negative genetic interaction withpdx1pyruvate dehydrogenase protein x component, Pdx1 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withpht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:18818364
positive genetic interaction withpmk1MAP kinase Pmk1 Positive GeneticPMID:18818364
negative genetic interaction withpmp1dual-specificity MAP kinase phosphatase Pmp1 Negative GeneticPMID:18818364
negative genetic interaction withpng2ING family homolog Png2 Negative GeneticPMID:18818364
negative genetic interaction withpph3serine/threonine protein phosphatase, PP4 complex subunit Pph3 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withppk16serine/threonine protein kinase Ppk16 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withppk8serine/threonine protein kinase Ppk8 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withptc2protein phosphatase 2C Ptc2 Negative GeneticPMID:18818364
negative genetic interaction withptf1Mst2 histone acetytransferase acytyltransferase complex, predicted phosphoric monoester hydrolase Ptf1 Negative GeneticPMID:18818364
negative genetic interaction withrad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Negative GeneticPMID:18818364
negative genetic interaction withrad57RecA family ATPase Rad57/Rhp57 Negative GeneticPMID:18818364
negative genetic interaction withrcd1RNA-binding protein, CCR4-NOT complex subunit Rcd1 Negative GeneticPMID:18818364
negative genetic interaction withreb1RNA polymerase I transcription termination factor/ RNA polymerase II transcription factor Reb1 Negative GeneticPMID:18818364
negative genetic interaction withrem1meiosis-specific cyclin Rem1 Negative GeneticPMID:18818364
negative genetic interaction withrev7DNA polymerase zeta Rev7 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withrhn1RNA polymerase II transcription termination factor homolog Negative GeneticPMID:18818364
negative genetic interaction withrhp26SNF2 family ATP-dependent DNA helicase Rhp26 Negative GeneticPMID:18818364
negative genetic interaction withrhp7Rad7 homolog Rhp7 Negative GeneticPMID:18818364
negative genetic interaction withrnf10ubiquitin-protein ligase E3 implicated in transcription (predicted) Negative GeneticPMID:18818364
negative genetic interaction withrox3mediator complex subunit Med19/Rox3 Negative GeneticPMID:18818364
negative genetic interaction withrpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:18818364
positive genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:18818364
negative genetic interaction withrsv2transcription factor Rsv2 Negative GeneticPMID:18818364
negative genetic interaction withsdc1Dpy-30 domain protein Sdc1 Negative GeneticPMID:18818364
negative genetic interaction withsdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:18818364
negative genetic interaction withset5histone lysine methyltransferase Set5 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withset9histone lysine H3-K20 methyltransferase Set9 Negative GeneticPMID:18818364
positive genetic interaction withsgf29SAGA complex subunit Sgf29 Positive GeneticPMID:18818364
negative genetic interaction withsir2Sirtuin family histone deacetylase Sir2 Negative GeneticPMID:18818364
positive genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:18818364
positive genetic interaction withSPAC23A1.07ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:18818364
negative genetic interaction withSPAC323.03cSchizosaccharomyces specific protein Negative GeneticPMID:18818364
negative genetic interaction withSPAC3A11.03methyltransferase (predicted) Negative GeneticPMID:18818364
negative genetic interaction withSPBC16C6.05mitochondrial translation initiation factor (predicted) Negative GeneticPMID:18818364
negative genetic interaction withSPBC16G5.03ubiquitin-protein ligase E3 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withSPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:18818364
negative genetic interaction withSPBC839.03cneddylation protein Dcn1 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withSPCC18.10pyridoxine-pyridoxal-pyridoxamine kinase (predicted) Negative GeneticPMID:18818364
negative genetic interaction withSPCC645.13transcription elongation regulator (predicted) Negative GeneticPMID:18818364
negative genetic interaction withspn1mitotic septin Spn1 Negative GeneticPMID:18818364
negative genetic interaction withsrb11cyclin CycC, Srb mediator subunit Srb11 Negative GeneticPMID:18818364
negative genetic interaction withssu72phosphoric ester hydrolase Ssu72 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withsum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:18818364
positive genetic interaction withswc5Swr1 complex subunit Swc5 Positive GeneticPMID:18818364
positive genetic interaction withswd1Set1C complex subunit Swd1 Positive GeneticPMID:18818364
negative genetic interaction withsyj2inositol-polyphosphate 5-phosphatase, synaptojanin homolog 2 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withtea2kinesin-like protein Tea2 Negative GeneticPMID:18818364
positive genetic interaction withtfs1transcription elongation factor TFIIS Positive GeneticPMID:18818364
negative genetic interaction withtma20RNA-binding protein Tma20 (predicted) Negative GeneticPMID:18818364
positive genetic interaction withtrm13tRNA 2'-O-methyltransferase Trm13 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withubp3ubiquitin C-terminal hydrolase Ubp3 Positive GeneticPMID:18818364
negative genetic interaction withufd2ubiquitin-protein ligase E4 Ufd2 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withura5orotate phosphoribosyltransferase Ura5 Negative GeneticPMID:18818364
negative genetic interaction withutp16U3 snoRNP-associated protein Utp16 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withutp4U3 snoRNP protein Utp4 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withyfh7uridine kinase Yfh7 (predicted) Negative GeneticPMID:18818364
External References
Database Identifier Description
NBRP SPBC15D4.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC15D4.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC15D4.10c BioGRID Interaction Datasets
Expression Viewer SPBC15D4.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC15D4.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC15D4.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC15D4.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC15D4.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC15D4.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC15D4.10c Cell Cycle Data
GEO SPBC15D4.10c GEO profiles
PInt SPBC15D4.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC15D4.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC15D4.10c Fission yeast phenotypic data & analysis
SPD / RIKEN24/24A05Orfeome Localization Data
UniProtKB/SwissProtO74315Nucleoporin-like protein amo1
ModBaseO74315Database of comparative protein structure models
STRINGO74315Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596250nuclear rim protein Amo1
RefSeq mRNANM_001022169972h- nuclear rim protein Amo1 (amo1), mRNA
European Nucleotide ArchiveCAA20485.1ENA Protein Mapping
UniParcUPI000006A222UniProt Archive

Literature for amo1

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015