spn3 (SPBC16A3.01)


Gene Standard Namespn3 Characterisation Statuspublished
Systematic IDSPBC16A3.01 Feature Typeprotein coding
SynonymsSPBC543.01c Name Description
Productseptin Spn3 Product Size412aa, 46.66 kDa
Genomic Location Chromosome II, 4300961-4298727 (2235nt); CDS:4300569-4299042 (1528nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005525GTP bindingIEAUniProtKB-KW:KW-0342GO_REF:0000037113
GO:0005515protein bindingIPIspn4PMID:15385632887
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000920cytokinetic cell separationIMPPMID:1538563224
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032153cell division siteIDAPMID:16823372292
GO:0005737cytoplasmIDAPMID:168233724198
GO:0005829cytosolIDAPMID:168233722317
GO:0031105septin complexIDAPMID:153856327
GO:0005940septin ringIDAPMID:153856325
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyspn3ΔNullPECO:0000005, PECO:0000137PMID:236978063755
Microscopyspn3ΔNullPMID:20473289
no_namePMID:15385632

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001490inviable elongated vegetative cellMicroscopyspn3ΔNullPECO:0000004, PECO:0000137PMID:23697806368
penetrance FYPO_EXT:0000001
FYPO:0001510viable vegetative cell, abnormal cell shape, normal cell sizeMicroscopyspn3ΔNullPECO:0000137, PECO:0000005PMID:23697806215
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000001
Target Of
RelationshipGeneProductReference
localized by spn1 septin Spn1 PMID:15385632
localized by spn4 septin Spn4 PMID:15385632
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
143009614300263
243000364299858
342997944298727

UTRs

Region Start End Reference
three_prime_UTR42990414298727PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00735 Pfam IPR000038 Cell division protein GTP binding 49 328 7
PTHR18884:SF16 HMMPANTHER 40 386 1
PTHR18884 HMMPANTHER IPR000038 Cell division protein GTP binding 40 386 7
G3DSA:3.40.50.300 Gene3D 48 229 279
SSF52540 SuperFamily IPR027417 45 295 294
Coil ncoils Rabaptin coiled-coil domain 361 385 975
Low complexity (SEG) seg 365 410
PIRSF006698 PIRSF IPR016491 Septin 28 410 7

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.25 Da
Charge -14.50
Isoelectric point 4.78
Molecular weight 46.66 kDa
Number of residues 412
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residuePMID:195477441192
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
7678.88during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
8983.87during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
2.9during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi only603
conserved in fungi4564
conserved in eukaryotes4482
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
prp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
SPBC1105.08EMP70 family Negative GeneticPMID:22681890
vps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
pyp1tyrosine phosphatase Pyp1 Negative GeneticPMID:22681890
elp3elongator complex, histone acetyltransferase subunit Elp3 (predicted) Negative GeneticPMID:22681890
pom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
ste20Rictor homolog, Ste20 Negative GeneticPMID:22681890
SPBC4B4.04translation initiation factor eIF2A (predicted) Negative GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
sty1MAP kinase Sty1 Negative GeneticPMID:22681890
pmk1MAP kinase Pmk1 Negative GeneticPMID:22681890
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
SPCPB16A4.02cconserved fungal protein Negative GeneticPMID:22681890
clr1cryptic loci regulator Clr1 Negative GeneticPMID:22681890
rgf3RhoGEF Rgf3 Phenotypic SuppressionPMID:16291723
erg5C-22 sterol desaturase Erg5 Negative GeneticPMID:22681890
ypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
sts5RNB-like protein Positive GeneticPMID:22681890
SPCC70.06nuclear export factor (predicted) Negative GeneticPMID:22681890
hrp3ATP-dependent DNA helicase Hrp3 Positive GeneticPMID:22681890
dsc5UBX domain containing protein required for Sre1 cleavage Negative GeneticPMID:22681890
rpb9DNA-directed RNA polymerase II complex subunit Rpb9 Negative GeneticPMID:22681890
mcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
sid2NDR kinase Sid2 Synthetic RescuePMID:16415366
vps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
mkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
ubp2ubiquitin C-terminal hydrolase Ubp2 Positive GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
spn4septin Spn4 Two-hybridPMID:15385632
Affinity Capture-MS
spn1septin Spn1 Affinity Capture-MSPMID:15385632
External References
Database Identifier Description
NBRP SPBC16A3.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC16A3.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC16A3.01 BioGRID Interaction Datasets
Expression Viewer SPBC16A3.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC16A3.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC16A3.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC16A3.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC16A3.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC16A3.01 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC16A3.01 Cell Cycle Data
GEO SPBC16A3.01 GEO profiles
PInt SPBC16A3.01 Protein-Protein Interaction Predictor (Bähler Lab)
SPD / RIKEN33/33B05Orfeome Localization Data
EntrezGene2540029septin Spn3
WikiGene2540029septin Spn3
UniProtKB/SwissProtP48008Septin homolog spn3
ModBaseP48008Database of comparative protein structure models
StringP48008Network display of known and predicted interactions and functional associations
RefSeq_mRNA_predictedXM_001713100972h- septin Spn3 (spn3), mRNA
RefSeq_peptide_predictedXP_001713152septin Spn3
European Nucleotide ArchiveAAB53691ENA Protein Mapping
European Nucleotide ArchiveCAA16852ENA Protein Mapping
UniParcUPI0000135E54UniProt Archive

Literature for spn3

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014