spn3 (SPBC16A3.01)


Gene Standard Namespn3 Characterisation Statuspublished
Systematic IDSPBC16A3.01 Feature Typeprotein coding
SynonymsSPBC543.01c Name Description
Productseptin Spn3 Product Size412aa, 46.66 kDa
Genomic Location Chromosome II, 4300961-4298727 (2235nt); CDS:4300569-4299042 (1528nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
GTP binding113
Annotation ExtensionEvidenceWith/FromReference
protein binding831
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

cytokinesis


Term NameCount
mitotic cytokinetic cell separation23
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site301
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4205
Annotation ExtensionEvidenceWith/FromReference
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
septin complex7
Annotation ExtensionEvidenceWith/FromReference
septin ring5
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003848sensitive to hydrazinocurcuminCell growth assayspn3+/spn3- (heterozygous diploid)Not specifiedPECO:0000103, PECO:0000137PMID:183092708
FYPO:0002060viable vegetative cell populationMicroscopynot recorded (unrecorded)PMID:153856323759
Microscopyspn3ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopyspn3ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001490inviable elongated vegetative cell393
penetrance FYPO_EXT:0000001Microscopyspn3ΔNullPECO:0000004, PECO:0000137PMID:23697806
FYPO:0001510viable vegetative cell, abnormal cell shape, normal cell size215
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000001
Microscopyspn3ΔNullPECO:0000137, PECO:0000005PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in cnb1 calcineurin regulatory subunit (calcineurin B) PMID:23956092
FYPO affected by mutation in ppb1 calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 PMID:23956092
GO localized by spn1 septin Spn1 PMID:15385632
GO localized by spn4 septin Spn4 PMID:15385632
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
143009614300263
243000364299858
342997944298727

UTRs

Region Coordinates Reference
three_prime_UTR4299041..4298727PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00735 Pfam IPR000038 Cell division protein GTP binding 49 328 7
PTHR18884 HMMPANTHER IPR000038 Cell division protein GTP binding 40 386 7
PTHR18884:SF16 HMMPANTHER 40 386 1
3.40.50.300 Gene3D 48 229 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 45 295 294
Coil ncoils Rabaptin coiled-coil domain 361 385 968
PIRSF006698 PIRSF IPR016491 Septin 28 410 6

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.25 Da
Charge -14.50
Isoelectric point 4.78
Molecular weight 46.66 kDa
Number of residues 412
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7534during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
8622during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
7638during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
8217during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
7678.88during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
7665during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
8983.87during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.9during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only623
conserved in fungi4600
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
spn4septin Spn4 Affinity Capture-MSPMID:15385632
Two-hybrid
spn3septin Spn3 Affinity Capture-WesternPMID:15385632
spt20SAGA complex subunit Spt20 Affinity Capture-WesternPMID:25015293
spn1septin Spn1 Affinity Capture-MSPMID:15385632
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
prp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
SPBC1105.08EMP70 family Negative GeneticPMID:22681890
vps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
pyp1tyrosine phosphatase Pyp1 Negative GeneticPMID:22681890
xap5xap-5-like protein Negative GeneticPMID:24957674
elp3elongator complex subunit Elp3 (predicted) Negative GeneticPMID:22681890
pom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
ste20Rictor homolog, Ste20 Negative GeneticPMID:22681890
SPBC4B4.04translation initiation factor eIF2A (predicted) Negative GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
sty1MAP kinase Sty1 Negative GeneticPMID:22681890
pmk1MAP kinase Pmk1 Negative GeneticPMID:22681890
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
SPCPB16A4.02cconserved fungal protein Negative GeneticPMID:22681890
clr1cryptic loci regulator Clr1 Negative GeneticPMID:22681890
rgf3RhoGEF Rgf3 Phenotypic SuppressionPMID:16291723
erg5C-22 sterol desaturase Erg5 Negative GeneticPMID:22681890
ypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
sts5RNB-like protein Positive GeneticPMID:22681890
SPCC70.06nuclear export factor (predicted) Negative GeneticPMID:22681890
hrp3ATP-dependent DNA helicase Hrp3 Positive GeneticPMID:22681890
dsc5UBX domain containing protein required for Sre1 cleavage Negative GeneticPMID:22681890
rpb9DNA-directed RNA polymerase II complex subunit Rpb9 Negative GeneticPMID:22681890
mcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
sid2NDR kinase Sid2 Synthetic RescuePMID:16415366
vps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
mkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
ubp2ubiquitin C-terminal hydrolase Ubp2 Positive GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC16A3.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC16A3.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC16A3.01 BioGRID Interaction Datasets
Expression Viewer SPBC16A3.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC16A3.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC16A3.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC16A3.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC16A3.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC16A3.01 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC16A3.01 Cell Cycle Data
GEO SPBC16A3.01 GEO profiles
PInt SPBC16A3.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC16A3.01 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC16A3.01 Fission yeast phenotypic data & analysis
SPD / RIKEN33/33B05Orfeome Localization Data
UniProtKB/SwissProtP48008Septin homolog spn3
ModBaseP48008Database of comparative protein structure models
STRINGP48008Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001713100972h- septin Spn3 (spn3), mRNA
RefSeq PeptideXP_001713152septin Spn3
European Nucleotide ArchiveU29889ENA EMBL mapping
European Nucleotide ArchiveCAA16852.1ENA Protein Mapping
UniParcUPI0000135E54UniProt Archive

Literature for spn3

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014