spn3 (SPBC16A3.01)


Gene Standard Namespn3 Characterisation Statuspublished
Systematic IDSPBC16A3.01 Feature Typeprotein coding
SynonymsSPBC543.01c Name Description
Productmitotic septin Spn3 Product Size412aa, 46.66 kDa
Genomic Location Chromosome II, 4300961-4298727 (2235nt); CDS:4300569-4299042 (1528nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
GTP binding113
Annotation ExtensionEvidenceWith/FromReference
protein binding863
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

mitotic cytokinesis


Term NameCount
mitotic cytokinesis106
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site313
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4213
Annotation ExtensionEvidenceWith/FromReference
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
septin complex4
Annotation ExtensionEvidenceWith/FromReference
septin ring7
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
increased septation indexspn3Δ61
sensitive to hydrazinocurcuminspn3+/spn3- (heterozygous diploid)8
viable vegetative cell population3837
spn3Δ

Cell Phenotype

Term NameGenotypesCount
decreased protein localization to medial cortex during vegetative growthspn3Δ7
inviable elongated vegetative cellspn3Δ416
viable vegetative cell, abnormal cell shape, normal cell sizespn3Δ215

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
increased septation indexpxl1Δ, spn3Δ
rho4Δ, spn3Δ
Target Of
OntologyRelationshipGeneProduct
FYPO localization affected by mutation in cnb1 calcineurin regulatory subunit (calcineurin B)
FYPO localization affected by mutation in ppb1 calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1
GO localized by spn1 mitotic septin Spn1
GO localized by spn4 mitotic septin Spn4
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4300961..4300263, 4300036..4299858, 4299794..4298727
Intron4300262..4300037, 4299857..4299795
5' UTR4300961..4300570SP29889
3' UTR4299041..4298727PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00735 Pfam IPR030379 Septin-type guanine nucleotide-binding (G) domain 49 328 7
PS51719 Prosite Profiles IPR030379 Septin-type guanine nucleotide-binding (G) domain 49 325 7
PTHR18884 HMMPANTHER IPR016491 Septin 26 386 7
PTHR18884:SF52 HMMPANTHER 26 386 3
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 48 229 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 45 295 294
Coil ncoils Predicted coiled-coil protein (DUF2205) 361 384 1057
PIRSF006698 PIRSF IPR016491 Septin 8 412 7

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.25 Da
Charge -14.50
Codon Adaptation Index 0.40
Isoelectric point 4.78
Molecular weight 46.66 kDa
Number of residues 412
Modifications

Protein Modifications

Term NameResidueCount
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for spn3 (SPBC16A3.01)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7534during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8622during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
7638during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8217during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
7678.88during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
7665during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8983.87during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.9during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only618
conserved in fungi4605
conserved in eukaryotes4515
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC16A3.01 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withspn4mitotic septin Spn4 Two-hybridPMID:15385632
affinity capturesspn1mitotic septin Spn1 Affinity Capture-MSPMID:15385632
affinity capturesspt20SAGA complex subunit Spt20 Affinity Capture-WesternPMID:25015293
affinity capturesspn3mitotic septin Spn3 Affinity Capture-WesternPMID:15385632
affinity capturesspn4mitotic septin Spn4 Affinity Capture-MSPMID:15385632
affinity captured byrho4Rho family GTPase Rho4 Affinity Capture-WesternPMID:25724972
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC16A3.01 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withsts5RNB-like protein Positive GeneticPMID:22681890
positive genetic interaction withubp2ubiquitin C-terminal hydrolase Ubp2 Positive GeneticPMID:22681890
positive genetic interaction withhrp3ATP-dependent DNA helicase Hrp3 Positive GeneticPMID:22681890
negative genetic interaction withsnf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
negative genetic interaction withypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
negative genetic interaction withrpb9DNA-directed RNA polymerase II complex subunit Rpb9 Negative GeneticPMID:22681890
negative genetic interaction withvps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc4 Negative GeneticPMID:22681890
negative genetic interaction withmcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withelp3elongator complex subunit Elp3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
negative genetic interaction withpmk1MAP kinase Pmk1 Negative GeneticPMID:22681890
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
negative genetic interaction withste20Rictor homolog, Ste20 Negative GeneticPMID:22681890
negative genetic interaction witherg5C-22 sterol desaturase Erg5 Negative GeneticPMID:22681890
negative genetic interaction withvps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
negative genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
negative genetic interaction withemp70EMP70 family endosomal transport protein Emp70 Negative GeneticPMID:22681890
negative genetic interaction withmkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
negative genetic interaction withclr1SHREC complex subunit Clr1 Negative GeneticPMID:22681890
negative genetic interaction withdsc5UBX domain containing protein required for Sre1 cleavage Negative GeneticPMID:22681890
negative genetic interaction withSPCC70.06nuclear export factor (predicted) Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
negative genetic interaction withSPCPB16A4.02cconserved fungal protein Negative GeneticPMID:22681890
negative genetic interaction witheif21translation initiation factor eIF2A (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpyp1tyrosine phosphatase Pyp1 Negative GeneticPMID:22681890
negative genetic interaction withpom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
negative genetic interaction withprp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
negative genetic interaction withsty1MAP kinase Sty1 Negative GeneticPMID:22681890
synthetically rescuessid2NDR kinase Sid2 Synthetic RescuePMID:16415366
rescuesrgf3RhoGEF Rgf3 Phenotypic SuppressionPMID:16291723
enhances phenotype ofrho4Rho family GTPase Rho4 Phenotypic EnhancementPMID:25724972
External References
Database Identifier Description
NBRP SPBC16A3.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC16A3.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC16A3.01 BioGRID Interaction Datasets
Expression Viewer SPBC16A3.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC16A3.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC16A3.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC16A3.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC16A3.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC16A3.01 Transcriptome Viewer (Bähler Lab)
GEO SPBC16A3.01 GEO profiles
PInt SPBC16A3.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC16A3.01 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC16A3.01 Fission yeast phenotypic data & analysis
Cyclebase SPBC16A3.01.1 Cell Cycle Data
SPD / RIKEN33/33B05Orfeome Localization Data
UniProtKB/SwissProtP48008Septin homolog spn3
ModBaseP48008Database of comparative protein structure models
STRINGP48008Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001713100972h- septin Spn3 (spn3), mRNA
RefSeq PeptideXP_001713152septin Spn3

Literature for spn3

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016