rrf1 (SPBC1709.09)


Gene Standard Namerrf1 Characterisation Statuspublished
Systematic IDSPBC1709.09 Feature Typeprotein coding
Synonyms Name Description
Productmitochondrial translation termination factor Rrf1 Product Size244aa, 27.69 kDa
Genomic Location Chromosome II, 1118360-1119457 (1098nt); CDS:1118561-1119295 (735nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
translation termination factor activity6
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitochondrial translational termination4
Annotation ExtensionEvidenceWith/FromReference
ribosome disassembly3
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
mitochondrion751
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencyrrf1Δ272
sensitive to tacrolimusrrf1Δ81
sensitive to tamoxifenrrf1Δ58
viable vegetative cell populationrrf1Δ3840

Cell Phenotype

Term NameGenotypesCount
abnormal cellular respiration16
abnormal microtubule cytoskeleton morphology during mitotic interphaserrf1Δ186
viable vegetative cell with normal cell morphologyrrf1Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1118360..1119457
mRNA1118360..1119457
5' UTR1118360..1118560PMID:21511999
CDS1118561..1119295
3' UTR1119296..1119457PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01765 Pfam IPR023584 Ribosome recycling factor domain 100 243 1
1.10.132.20 Gene3D IPR023584 Ribosome recycling factor domain 126 242 1
SSF55194 SuperFamily IPR023584 Ribosome recycling factor domain 55 242 1
Coil ncoils Predicted coiled-coil protein (DUF2205) 182 206 1057

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.48 Da
Charge 18.00
Codon Adaptation Index 0.34
Isoelectric point 10.47
Molecular weight 27.69 kDa
Number of residues 244
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS118
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S118 PMID:21712547
O-phospho-L-threonine 701
present during mitotic M phaseT106
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T106 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for rrf1 (SPBC1709.09)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
19307during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
15967during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
19934during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
16920during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
18495during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4779.56during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2280.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.55during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.11during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by human MRRF PMID:18782833
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in bacteria1001
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC1709.09 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withhip1hira protein, histone chaperone Hip1 Positive GeneticPMID:22681890
positive genetic interaction withatp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withcsn1COP9/signalosome complex subunit Csn1 Positive GeneticPMID:22681890
positive genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Positive GeneticPMID:22681890
positive genetic interaction withbob1prefoldin subunit 5 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withuap2U2 snRNP-associated protein Uap2 Positive GeneticPMID:22681890
positive genetic interaction withphp5CCAAT-binding factor complex subunit Php5 Positive GeneticPMID:22681890
positive genetic interaction withssm4dynactin microtubule-binding subunit, p150-Glued Ssm4 Positive GeneticPMID:22681890
positive genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
positive genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
positive genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
positive genetic interaction withsif1Sad1 interacting factor 1 Positive GeneticPMID:22681890
positive genetic interaction withSPBC1347.08cribonuclease H2 complex subunit (predicted) Positive GeneticPMID:22681890
positive genetic interaction withcdt2WD repeat protein Cdt2 Positive GeneticPMID:22681890
negative genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withtor1serine/threonine protein kinase Tor1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC1709.09 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1709.09 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1709.09 BioGRID Interaction Datasets
Expression Viewer SPBC1709.09 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1709.09 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1709.09 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1709.09 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1709.09 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1709.09 Transcriptome Viewer (Bähler Lab)
GEO SPBC1709.09 GEO profiles
PInt SPBC1709.09 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1709.09 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1709.09 Fission yeast phenotypic data & analysis
Cyclebase SPBC1709.09.1 Cell Cycle Data
SPD / RIKEN43/43A07Orfeome Localization Data
UniProtKB/SwissProtO74734Putative ribosomal recycling factor, mitochondrial
ModBaseO74734Database of comparative protein structure models
STRINGO74734Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595442mitochondrial translation termination factor Rrf1
RefSeq mRNANM_001021351972h- mitochondrial translation termination factor Rrf1 (rrf1), mRNA

Literature for rrf1

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016