zfs1 (SPBC1718.07c)


Gene Standard Namezfs1 Characterisation Statuspublished
Systematic IDSPBC1718.07c Feature Typeprotein coding
Synonymsmoc4 Name DescriptionMulticopy suppressor of Overexpressed Cyr1
ProductCCCH tandem zinc finger protein, human Tristetraprolin homolog Zfs1, involved in mRNA catabolism Product Size404aa, 43.44 kDa
Genomic Location Chromosome II, 3487862-3482848 (5015nt); CDS:3484668-3483454 (1215nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
double-stranded DNA binding141
Annotation ExtensionEvidenceWith/FromReference
metal ion binding751
Annotation ExtensionEvidenceWith/FromReference
mRNA 3'-UTR AU-rich region binding3
Annotation ExtensionEvidenceWith/FromReference
protein binding868
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth at high temperaturezfs1Δ172
decreased conjugation frequencyzfs1Δ51
H389I (H389I)
H351I (H351I)
H351I,H389I (H351I, H389I)
decreased mating efficiencyzfs1Δ272
decreased sporulation frequencyzfs1-/zfs1- diploid (homozygous diploid)103
normal growth on calcium ionzfs1Δ8
sensitive to cobaltzfs1Δ57
sensitive to hydroxyureazfs1Δ580
sensitive to low pHzfs1Δ5
sensitive to thiabendazolezfs1Δ326
slow vegetative cell population growthzfs1Δ357
viable vegetative cell populationzfs1Δ3840

Cell Phenotype

Term NameGenotypesCount
abnormal microtubule cytoskeleton morphology during mitotic interphasezfs1Δ186
chromosome loss during mitotic chromosome segregationzfs1Δ12
cut following normal mitotic chromosome condensationzfs1Δ9
decreased RNA catabolic process during vegetative growth10
affecting arz1zfs1Δ
decreased RNA level during nitrogen starvation43
affecting mam2zfs1Δ
affecting mat2-Pizfs1Δ
elongated telomereszfs1Δ173
increased mRNA level during vegetative growth81
affecting tpp1zfs1Δ
affecting nth1zfs1Δ
affecting rod1zfs1Δ
affecting SPAC343.06czfs1Δ
affecting spt20zfs1Δ
affecting SPAC7D4.12czfs1Δ
affecting mug63zfs1Δ
affecting gmh6zfs1Δ
affecting nep1zfs1Δ
affecting SPBC20F10.03zfs1Δ
affecting kin17zfs1Δ
affecting arz1zfs1Δ
mitotic G1/S transition delayzfs1Δ13
normal protein localization to cell division site32
affecting myo2zfs1Δ
normal RNA level during nitrogen starvation8
affecting ste11zfs1Δ
affecting mat2-Pczfs1Δ
normal spore germinationzfs1Δ21
viable small vegetative cellzfs1Δ51
viable vegetative cell with abnormal cell shapezfs1Δ700

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased mating efficiencymra1+ (wild type), zfs1Δ

Cell Phenotype

Term NameGenotypes
sterilebyr3+, zfs1Δ
zfs1+, byr3Δ
byr2Δ, zfs1+
zfs1+, gpa1Δ
byr1Δ, zfs1+
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3487862..3482848
mRNA3487862..3482848
5' UTR3487862..3484669PMID:21511999
CDS3484668..3483454
3' UTR3483453..3482848PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00642 Pfam IPR000571 Zinc finger, CCCH-type 327 352 6
PF00642 Pfam IPR000571 Zinc finger, CCCH-type 365 390 6
SM00356 SMART IPR000571 Zinc finger, CCCH-type 364 391 8
SM00356 SMART IPR000571 Zinc finger, CCCH-type 326 353 8
PS50103 Prosite Profiles IPR000571 Zinc finger, CCCH-type 326 354 14
PS50103 Prosite Profiles IPR000571 Zinc finger, CCCH-type 364 392 14
PTHR12547 HMMPANTHER 259 404 1
1m9oA00 Gene3D IPR000571 Zinc finger, CCCH-type 325 359 6
1m9oA00 Gene3D IPR000571 Zinc finger, CCCH-type 360 392 6
SSF90229 SuperFamily IPR000571 Zinc finger, CCCH-type 326 357 7
SSF90229 SuperFamily IPR000571 Zinc finger, CCCH-type 361 392 7

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000137zinc finger proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000137
PBO:0000416zf-CCCH typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000416

Protein Properties

Ave. residue weight 107.51 Da
Charge 22.00
Codon Adaptation Index 0.45
Isoelectric point 9.84
Molecular weight 43.44 kDa
Number of residues 404
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during cellular response to thiabendazole, mitotic M phaseS403
present during mitotic M phaseS280
present during mitotic M phaseS229
present during mitotic M phaseS275
level fluctuates during mitotic cell cycleS229
S403
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S403 PMID:18257517
present during mitotic M phase experimental evidence S280 PMID:21712547
present during mitotic M phase experimental evidence S403 PMID:21712547
present during mitotic M phase experimental evidence S229 PMID:21712547
present during mitotic M phase experimental evidence S275 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence S229 PMID:24763107
experimental evidence S403 PMID:24763107
O-phospho-L-threonine 701
present during mitotic M phaseT283
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T283 PMID:21712547
phosphorylated residue 1931
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0072690Northern assay evidencePMID:8534915
increased during GO:0006995Northern assay evidencePMID:8534915
increased during GO:0006995experimental evidencePMID:8534915

Quantitative Gene Expression

View graphical display of gene expression data for zfs1 (SPBC1718.07c)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2506
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC1718.07c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withpdc101pyruvate decarboxylase (predicted) Two-hybridPMID:19682301
binds activation domain construct withrpp20260S acidic ribosomal protein A4 Two-hybridPMID:19682301
binds activation domain construct withebp2rRNA processing protein Ebp2 (predicted) Two-hybridPMID:19682301
binds activation domain construct withmse1mitochondrial glutamyl-tRNA ligase Mse1 (predicted) Two-hybridPMID:19682301
binds activation domain construct witheft201translation elongation factor 2 (EF-2) Eft2,A Two-hybridPMID:19682301
binds activation domain construct witheno101enolase (predicted) Two-hybridPMID:19682301
binds activation domain construct withrpl50160S ribosomal protein L5 (predicted) Two-hybridPMID:19682301
binds activation domain construct withtdh1glyceraldehyde-3-phosphate dehydrogenase Tdh1 Two-hybridPMID:19682301
binds activation domain construct withtef101translation elongation factor EF-1 alpha Ef1a-a Two-hybridPMID:19682301
binds activation domain construct withrpl320260S ribosomal protein L32 (predicted) Two-hybridPMID:19682301
binds activation domain construct withtpx1thioredoxin peroxidase Tpx1 Two-hybridPMID:19682301
binds activation domain construct withfba1fructose-bisphosphate aldolase Fba1 Two-hybridPMID:19682301
binds activation domain construct withcrb3WD repeat protein Crb3 Two-hybridPMID:19682301
binds activation domain construct withufd2ubiquitin-protein ligase E4 Ufd2 (predicted) Two-hybridPMID:19682301
binds activation domain construct withsec6exocyst complex subunit Sec6 Two-hybridPMID:26771498
binds activation domain construct withppn1mRNA cleavage and polyadenylation specificity factor complex associated protein Two-hybridPMID:19682301
binds activation domain construct withrpl120160S ribosomal protein L12.1/L12A Two-hybridPMID:19682301
binds activation domain construct withsrp54signal recognition particle subunit Srp54 Two-hybridPMID:19682301
binds activation domain construct withpsu1cell wall protein Psu1, beta-glucosidase (predicted) Two-hybridPMID:19682301
binds activation domain construct withpup220S proteasome complex subunit alpha 5, Pup2 (predicted) Two-hybridPMID:19682301
affinity capturesmus7DNA repair protein Mus7/Mms22 Affinity Capture-RNAPMID:22907753
affinity capturesecl3extender of the chronological lifespan protein Ecl3 Affinity Capture-RNAPMID:22907753
affinity capturespfl7cell surface glycoprotein (predicted), DIPSY family Affinity Capture-RNAPMID:22907753
affinity capturesarz1armadillo repeat containing, Zfs1 target number 1 Affinity Capture-RNAPMID:22907753
affinity capturescbf12CBF1/Su(H)/LAG-1 family transcription factor Cbf12 Affinity Capture-RNAPMID:22907753
affinity capturesstg1SM22/transgelin-like actin modulating protein Stg1 Affinity Capture-RNAPMID:22907753
affinity capturesSPAC589.03cSchizosaccharomyces specific protein Affinity Capture-RNAPMID:22907753
binds DNA-binding domain construct withatg11autophagy protein Atg11 Two-hybridPMID:26771498
binds DNA-binding domain construct withmoc3transcription factor Moc3 Two-hybridPMID:16273369
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC1718.07c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescuespac1double-strand-specific ribonuclease Pac1 Phenotypic SuppressionPMID:8534915
rescuescyr1adenylate cyclase Phenotypic SuppressionPMID:16273369
rescuesmoc3transcription factor Moc3 Phenotypic SuppressionPMID:16273369
rescuesras1GTPase Ras1 Phenotypic SuppressionPMID:8534915
rescuesmob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Phenotypic SuppressionPMID:10769201
rescued bymoc3transcription factor Moc3 Phenotypic SuppressionPMID:16273369
rescued bybyr1MAP kinase kinase Byr1 Phenotypic SuppressionPMID:8534915
rescued bygpa1G-protein alpha subunit Phenotypic SuppressionPMID:8534915
rescued bymra1rRNA (pseudouridine) methyltransferase Mra1 Phenotypic SuppressionPMID:9133664
rescued bybyr2MAP kinase kinase kinase Byr2 Phenotypic SuppressionPMID:8534915
rescued bycbf12CBF1/Su(H)/LAG-1 family transcription factor Cbf12 Phenotypic SuppressionPMID:22907753
rescued byras1GTPase Ras1 Phenotypic SuppressionPMID:8534915
phenotype enhanced bysol1SWI/SNF complex subunit Sol1 Phenotypic EnhancementPMID:22624651
phenotype enhanced byppa2serine/threonine protein phosphatase Ppa2 Phenotypic EnhancementPMID:22624651
phenotype enhanced byclp1Cdc14-related protein phosphatase Clp1/Flp1 Phenotypic EnhancementPMID:22624651
phenotype enhanced bywee1M phase inhibitor protein kinase Wee1 Phenotypic EnhancementPMID:10462526
enhances phenotype ofclp1Cdc14-related protein phosphatase Clp1/Flp1 Phenotypic EnhancementPMID:22624651
enhances phenotype ofsnf5SWI/SNF complex subunit Snf5 Phenotypic EnhancementPMID:22624651
enhances phenotype ofsol1SWI/SNF complex subunit Sol1 Phenotypic EnhancementPMID:22624651
enhances phenotype ofppa2serine/threonine protein phosphatase Ppa2 Phenotypic EnhancementPMID:22624651
enhances phenotype ofsty1MAP kinase Sty1 Phenotypic EnhancementPMID:22624651
enhances phenotype ofcdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Phenotypic EnhancementPMID:22624651
positive genetic interaction withpuf3pumilio family RNA-binding protein Puf3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withppk15serine/threonine protein kinase Ppk15 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withpli1SUMO E3 ligase Pli1 Positive GeneticPMID:22681890
positive genetic interaction withlsk1P-TEFb-associated cyclin-dependent protein kinase Lsk1 Positive GeneticPMID:22681890
positive genetic interaction withset1histone lysine methyltransferase Set1 Positive GeneticPMID:22681890
positive genetic interaction withpac10prefoldin subunit 3 Pac10 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withppk16serine/threonine protein kinase Ppk16 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withyfh7uridine kinase Yfh7 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Positive GeneticPMID:22681890
positive genetic interaction withndk1nucleoside diphosphate kinase Ndk1 Positive GeneticPMID:22681890
positive genetic interaction withSPCC18B5.05cphosphomethylpyrimidine kinase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withatg1autophagy and CVT pathway serine/threonine protein kinase Atg1 Positive GeneticPMID:22681890
positive genetic interaction withrad57RecA family ATPase Rad57/Rhp57 Positive GeneticPMID:22681890
positive genetic interaction withswd1Set1C complex subunit Swd1 Positive GeneticPMID:22681890
negative genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
negative genetic interaction withtsc1hamartin Negative GeneticPMID:22681890
negative genetic interaction withrxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
negative genetic interaction withace2transcription factor Ace2 Negative GeneticPMID:22681890
negative genetic interaction withpom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
negative genetic interaction withsck2serine/threonine protein kinase Sck2 Negative GeneticPMID:24463365
negative genetic interaction withppa2serine/threonine protein phosphatase Ppa2 Negative GeneticPMID:22681890
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:22681890
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withset9histone lysine H3-K20 methyltransferase Set9 Negative GeneticPMID:22681890
negative genetic interaction withSPBC19G7.04HMG box protein Negative GeneticPMID:22681890
negative genetic interaction withtsc2tuberin Negative GeneticPMID:22681890
negative genetic interaction withpht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:22681890
negative genetic interaction withmmb1mitochondrial microtubule binder Mmb1 Negative GeneticPMID:22681890
negative genetic interaction withmid1medial ring protein Mid1 Negative GeneticPMID:22681890
negative genetic interaction withpng2ING family homolog Png2 Negative GeneticPMID:22681890
negative genetic interaction withrpb7DNA-directed RNA polymerase complex II subunit Rpb7 Negative GeneticPMID:22681890
negative genetic interaction withfhl1forkhead transcription factor Fhl1 Negative GeneticPMID:22681890
negative genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc4 Negative GeneticPMID:21504829
negative genetic interaction withpar1protein phosphatase PP2A regulatory subunit B-56 Par1 Negative GeneticPMID:22681890
negative genetic interaction withphp3CCAAT-binding factor complex subunit Php3 Negative GeneticPMID:22681890
negative genetic interaction withers1RNA-silencing factor Ers1 Negative GeneticPMID:22681890
negative genetic interaction withtol13'(2'),5'-bisphosphate nucleotidase/inositol-1,4- bisphosphate 1-phosphatase Negative GeneticPMID:22681890
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
overexpression rescuescpp1protein farnesyltransferase beta subunit Cpp1 Dosage RescuePMID:16624901
overexpression rescuescdc16two-component GAP Cdc16 Dosage RescuePMID:10462526
overexpression rescuestsc2tuberin Dosage RescuePMID:16624901
synthetically rescuescdc11SIN component scaffold protein, centriolin ortholog Cdc11 Synthetic RescuePMID:10462526
synthetically rescuescdc7serine/threonine protein kinase Cdc7 Synthetic RescuePMID:10462526
synthetically rescuessid4SIN component scaffold protein Sid4 Synthetic RescuePMID:10462526
synthetically rescuessid2NDR kinase Sid2 Synthetic RescuePMID:10462526
synthetically rescuessid1PAK-related GC kinase Sid1 Synthetic RescuePMID:10462526
synthetically rescuescdc14SIN component Cdc14 Synthetic RescuePMID:10462526
synthetically rescuesspg1GTPase Spg1 Synthetic RescuePMID:10462526
External References
Database Identifier Description
NBRP SPBC1718.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1718.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1718.07c BioGRID Interaction Datasets
Expression Viewer SPBC1718.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1718.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1718.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1718.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1718.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1718.07c Transcriptome Viewer (Bähler Lab)
GEO SPBC1718.07c GEO profiles
PInt SPBC1718.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1718.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1718.07c Fission yeast phenotypic data & analysis
Cyclebase SPBC1718.07c.1 Cell Cycle Data
SPD / RIKEN32/32F07Orfeome Localization Data
UniProtKB/SwissProtP47979Zinc finger protein zfs1
ModBaseP47979Database of comparative protein structure models
STRINGP47979Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596453zinc finger protein
RefSeq mRNANM_001022372972h- zinc finger protein (zfs1), mRNA

Literature for zfs1

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016