zfs1 (SPBC1718.07c)

Gene Standard Namezfs1 Characterisation Statuspublished
Systematic IDSPBC1718.07c Feature Typeprotein coding
Synonymsmoc4 Name DescriptionMulticopy suppressor of Overexpressed Cyr1
ProductCCCH tandem zinc finger protein, human Tristetraprolin homolog Zfs1, involved in mRNA catabolism Product Size404aa, 43.44 kDa
Genomic Location Chromosome II, 3487862-3482848 (5015nt); CDS:3484668-3483454 (1215nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
double-stranded DNA binding26
Annotation ExtensionEvidenceWith/FromReference
metal ion binding747
Annotation ExtensionEvidenceWith/FromReference
mRNA 3'-UTR AU-rich region binding3
Annotation ExtensionEvidenceWith/FromReference
protein binding848
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth at high temperaturezfs1ΔNull151
decreased conjugation frequencyH351I (H351I)Not specified39
H351I,H389I (H351I, H389I)Not specified
H389I (H389I)Not specified
decreased mating efficiencyzfs1ΔNull247
expressivity FYPO_EXT:0000001zfs1ΔNull
normal growth on calcium ionzfs1ΔNull5
sensitive to cobalt56
expressivity FYPO_EXT:0000003zfs1ΔNull
sensitive to hydroxyurea518
expressivity FYPO_EXT:0000003zfs1ΔNull
sensitive to low pHzfs1ΔNull4
sensitive to thiabendazolezfs1ΔNull185
slow vegetative cell population growthzfs1ΔNull88
viable vegetative cell populationzfs1ΔNull3781

Cell Phenotype

Term NameAlleleExpressionCount
abnormal microtubule cytoskeleton morphology during mitotic interphase186
penetrance FYPO_EXT:0000002zfs1ΔNull
chromosome loss during mitotic chromosome segregationzfs1ΔNull7
cut following normal mitotic entryzfs1ΔNull7
decreased RNA catabolic process during vegetative growth10
expressivity FYPO_EXT:0000001, affecting arz1zfs1ΔNull
decreased RNA level during nitrogen starvation34
affecting mam2zfs1ΔNull
affecting mat2-Pizfs1ΔNull
decreased sporulationzfs1-/zfs1- diploid (homozygous diploid)Null65
elongated telomeres160
expressivity FYPO_EXT:0000003zfs1ΔNull
increased mRNA level during vegetative growth25
affecting tpp1zfs1ΔNull
affecting nth1zfs1ΔNull
affecting SPAC31A2.12zfs1ΔNull
affecting SPAC343.06czfs1ΔNull
affecting spt20zfs1ΔNull
affecting SPAC7D4.12czfs1ΔNull
affecting mug63zfs1ΔNull
affecting gmh6zfs1ΔNull
affecting nep1zfs1ΔNull
affecting SPBC20F10.03zfs1ΔNull
affecting SPBC365.09czfs1ΔNull
affecting arz1zfs1ΔNull
mitotic G1/S transition delayzfs1ΔNull10
normal protein localization to cell division site27
affecting myo2zfs1ΔNull
normal RNA level during nitrogen starvation10
affecting ste11zfs1ΔNull
affecting mat2-Pczfs1ΔNull
normal spore germinationzfs1ΔNull16
viable small vegetative cellzfs1ΔNull38
viable vegetative cell with abnormal cell shape472
penetrance FYPO_EXT:0000002zfs1ΔNull
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00642 Pfam IPR000571 Zinc finger, CCCH-type 327 352 6
PF00642 Pfam IPR000571 Zinc finger, CCCH-type 365 390 6
SM00356 SMART IPR000571 Zinc finger, CCCH-type 364 391 8
SM00356 SMART IPR000571 Zinc finger, CCCH-type 326 353 8
PS50103 Prosite Profiles IPR000571 Zinc finger, CCCH-type 326 354 14
PS50103 Prosite Profiles IPR000571 Zinc finger, CCCH-type 364 392 14
PTHR12547:SF18 HMMPANTHER 158 391 1
PTHR12547 HMMPANTHER 158 391 1
1m9oA00 Gene3D IPR000571 Zinc finger, CCCH-type 360 392 6
1m9oA00 Gene3D IPR000571 Zinc finger, CCCH-type 325 359 6
SSF90229 SuperFamily 326 357 7
SSF90229 SuperFamily 361 392 7

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000137zinc finger proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000137
PBO:0000416zf-CCCH typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000416

Protein Properties

Ave. residue weight 107.51 Da
Charge 22.00
Isoelectric point 9.84
Molecular weight 43.44 kDa
Number of residues 404

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS229
present during cellular response to thiabendazole, mitotic M phaseS403
present during mitotic M phaseS280
present during mitotic M phaseS275
level fluctuates during mitotic cell cycleS229
Annotation ExtensionEvidenceResidueReference
level fluctuates during mitotic cell cycle experimental evidence S229 PMID:24763107
present during mitotic M phase experimental evidence S229 PMID:21712547
present during mitotic M phase experimental evidence S275 PMID:21712547
present during mitotic M phase experimental evidence S280 PMID:21712547
experimental evidence S403 PMID:24763107
present during cellular response to thiabendazole IDA S403 PMID:18257517
present during mitotic M phase experimental evidence S403 PMID:21712547
O-phospho-L-threonine 693
present during mitotic M phaseT283
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T283 PMID:21712547
phosphorylated residue 1922
Annotation ExtensionEvidenceResidueReference
NAS PMID:18257517
IDA PMID:19547744
Gene Expression

Qualitative Gene Expression

RNA levelpresent during GO:0072690Northern assay evidencePMID:8534915
increased during GO:0006995experimental evidencePMID:8534915
increased during GO:0006995Northern assay evidencePMID:8534915

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
3.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633
Species Distribution
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
rpl50160S ribosomal protein L5 (predicted) Two-hybridPMID:19682301
tef101translation elongation factor EF-1 alpha Ef1a-a Two-hybridPMID:19682301
SPAC589.03cSchizosaccharomyces specific protein Affinity Capture-RNAPMID:22907753
pfl7cell surface glycoprotein (predicted), DIPSY family Affinity Capture-RNAPMID:22907753
eft202translation elongation factor 2 (EF-2) Eft2,B Two-hybridPMID:19682301
moc3transcription factor Moc3 Two-hybridPMID:16273369
ppn1PNUTS homologue, mRNA cleavage and polyadenylation specificity factor complex associated protein Two-hybridPMID:19682301
arz1armadillo repeat containing, Zfs1 target number 1 Affinity Capture-RNAPMID:22907753
mse1mitochondrial glutamyl-tRNA ligase Mse1 (predicted) Two-hybridPMID:19682301
rpl120260S ribosomal protein L12.1/L12A Two-hybridPMID:19682301
crb3WD repeat protein Crb3 Two-hybridPMID:19682301
pup220S proteasome complex subunit alpha 5, Pup2 (predicted) Two-hybridPMID:19682301
SPAC1F8.07cpyruvate decarboxylase (predicted) Two-hybridPMID:19682301
atg11autophagy protein Atg11 Two-hybridPMID:23695164
psu1cell wall protein Psu1, beta-glucosidase (predicted) Two-hybridPMID:19682301
ecl3extender of the chronological lifespan protein Ecl3 Affinity Capture-RNAPMID:22907753
eno101enolase (predicted) Two-hybridPMID:19682301
cbf12CBF1/Su(H)/LAG-1 family transcription factor Cbf12 Affinity Capture-RNAPMID:22907753
stg1SM22/transgelin-like actin modulating protein Stg1 Affinity Capture-RNAPMID:22907753
rpp20260S acidic ribosomal protein A4 Two-hybridPMID:19682301
tdh1glyceraldehyde-3-phosphate dehydrogenase Tdh1 Two-hybridPMID:19682301
ebp2rRNA processing protein Ebp2 (predicted) Two-hybridPMID:19682301
fba1fructose-bisphosphate aldolase Fba1 Two-hybridPMID:19682301
mus7DNA repair protein Mus7/Mms22 Affinity Capture-RNAPMID:22907753
rpl320260S ribosomal protein L32 (predicted) Two-hybridPMID:19682301
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
pht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:22681890
ppk16serine/threonine protein kinase Ppk16 (predicted) Positive GeneticPMID:22681890
atg1autophagy and CVT pathway serine/threonine protein kinase Atg1 Positive GeneticPMID:22681890
ndk1nucleoside diphosphate kinase Ndk1 Positive GeneticPMID:22681890
ace2transcription factor Ace2 Negative GeneticPMID:22681890
set1histone lysine methyltransferase Set1 Positive GeneticPMID:22681890
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
mra1rRNA (pseudouridine) methyltransferase Mra1 Phenotypic SuppressionPMID:9133664
clr4histone H3 lysine methyltransferase Clr4 Positive GeneticPMID:22681890
cdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Phenotypic EnhancementPMID:22624651
puf3RNA-binding protein Puf3 (predicted) Positive GeneticPMID:22681890
byr2MAP kinase kinase kinase Byr2 Phenotypic SuppressionPMID:8534915
cpp1protein farnesyltransferase beta subunit Cpp1 Dosage RescuePMID:16624901
lsk1P-TEFb-associated cyclin-dependent protein kinase Lsk1 Positive GeneticPMID:22681890
tsc1hamartin Negative GeneticPMID:22681890
mid1medial ring protein Mid1 Negative GeneticPMID:22681890
sty1MAP kinase Sty1 Phenotypic EnhancementPMID:22624651
swd1Set1C complex subunit Swd1 Positive GeneticPMID:22681890
rxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
cdc7serine/threonine protein kinase Cdc7 Synthetic RescuePMID:10462526
wee1M phase inhibitor protein kinase Wee1 Phenotypic EnhancementPMID:10462526
cdc11SIN component scaffold protein, centriolin ortholog Cdc11 Synthetic RescuePMID:10462526
clp1Cdc14-related protein phosphatase Clp1/Flp1 Phenotypic EnhancementPMID:22624651
ras1GTPase Ras1 Phenotypic SuppressionPMID:8534915
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
sol1SWI/SNF complex subunit Sol1 Phenotypic EnhancementPMID:22624651
SPBC19G7.04HMG box protein Negative GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Phenotypic EnhancementPMID:22624651
moc3transcription factor Moc3 Phenotypic SuppressionPMID:16273369
gpa1G-protein alpha subunit Phenotypic SuppressionPMID:8534915
ers1RNA-silencing factor Ers1 Negative GeneticPMID:22681890
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
cdc16two-component GAP Cdc16 Dosage RescuePMID:10462526
pli1SUMO E3 ligase Pli1 Positive GeneticPMID:22681890
rad57RecA family ATPase Rad57/Rhp57 Positive GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
sid1PAK-related GC kinase Sid1 Synthetic RescuePMID:10462526
tol13'(2'),5'-bisphosphate nucleotidase/inositol-1,4- bisphosphate 1-phosphatase Negative GeneticPMID:22681890
sck2serine/threonine protein kinase Sck2 Negative GeneticPMID:24463365
sre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
mob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Phenotypic SuppressionPMID:10769201
cbf12CBF1/Su(H)/LAG-1 family transcription factor Cbf12 Phenotypic SuppressionPMID:22907753
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
cdc14SIN component Cdc14 Synthetic RescuePMID:10462526
par1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
set9histone lysine H3-K20 methyltransferase Set9 Negative GeneticPMID:22681890
SPCC18B5.05cphosphomethylpyrimidine kinase (predicted) Positive GeneticPMID:22681890
png2ING family homolog Png2 Negative GeneticPMID:22681890
sid4SIN component scaffold protein Sid4 Synthetic RescuePMID:10462526
rpb7DNA-directed RNA polymerase complex II subunit Rpb7 Negative GeneticPMID:22681890
yfh7uridine kinase Yfh7 (predicted) Positive GeneticPMID:22681890
spg1GTPase Spg1 Synthetic RescuePMID:10462526
php3CCAAT-binding factor complex subunit Php3 Negative GeneticPMID:22681890
mad2mitotic spindle checkpoint protein Mad2 Synthetic LethalityPMID:12186944
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
prw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
sid2NDR kinase Sid2 Synthetic RescuePMID:10462526
pom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
cyr1adenylate cyclase Phenotypic SuppressionPMID:16273369
byr1MAP kinase kinase Byr1 Phenotypic SuppressionPMID:8534915
pac10prefoldin subunit 3 Pac10 (predicted) Positive GeneticPMID:22681890
fhl1forkhead transcription factor Fhl1 Negative GeneticPMID:22681890
pac1double-strand-specific ribonuclease Pac1 Phenotypic SuppressionPMID:8534915
ppa2serine/threonine protein phosphatase Ppa2 Phenotypic EnhancementPMID:22624651
Negative GeneticPMID:22681890
ppk15serine/threonine protein kinase Ppk15 (predicted) Positive GeneticPMID:22681890
tsc2tuberin Dosage RescuePMID:16624901
Negative GeneticPMID:22681890
mmb1mitochondrial microtubule binder Mmb1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC1718.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1718.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1718.07c BioGRID Interaction Datasets
Expression Viewer SPBC1718.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1718.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1718.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1718.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1718.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1718.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1718.07c Cell Cycle Data
GEO SPBC1718.07c GEO profiles
PInt SPBC1718.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1718.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1718.07c Fission yeast phenotypic data & analysis
SPD / RIKEN32/32F07Orfeome Localization Data
UniProtKB/SwissProtP47979Zinc finger protein zfs1
ModBaseP47979Database of comparative protein structure models
STRINGP47979Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596453zinc finger protein
RefSeq mRNANM_001022372972h- zinc finger protein (zfs1), mRNA
European Nucleotide ArchiveBAA08654.1ENA Protein Mapping
European Nucleotide ArchiveCAB75997.1ENA Protein Mapping
UniParcUPI000013C3B9UniProt Archive

Literature for zfs1

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015