pst3 (SPBC1734.16c)


Gene Standard Namepst3 Characterisation Statuspublished
Systematic IDSPBC1734.16c Feature Typeprotein coding
Synonyms Name Description
ProductSIN3 family co-repressor Pst3 Product Size1154aa, 132.88 kDa
Genomic Location Chromosome II, 1094818-1089163 (5656nt); CDS:1094653-1091098 (3556nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
chromatin remodeling159
Annotation ExtensionEvidenceWith/FromReference
chromatin silencing105
Annotation ExtensionEvidenceWith/FromReference
double-strand break repair via nonhomologous end joining7
Annotation ExtensionEvidenceWith/FromReference
histone deacetylation23
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
Rpd3L-Expanded complex15
Annotation ExtensionEvidenceWith/FromReference
Rpd3S complex16
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationpst3ΔNull1427

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclepst3ΔNull794
inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells87
penetrance FYPO_EXT:0000001pst3ΔNull
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
110948181094608
210945161089163

UTRs

Region Coordinates Reference
five_prime_UTR1094818..1094654PMID:21511999
three_prime_UTR1091097..1089163PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08295 Pfam IPR013194 Histone deacetylase interacting 497 590 3
PF02671 Pfam IPR003822 Paired amphipathic helix 428 469 3
PF02671 Pfam IPR003822 Paired amphipathic helix 133 179 3
PF02671 Pfam IPR003822 Paired amphipathic helix 275 320 3
SM00761 SMART IPR013194 Histone deacetylase interacting 492 592 3
PS51477 Prosite Profiles IPR003822 Paired amphipathic helix 403 472 3
PS51477 Prosite Profiles IPR003822 Paired amphipathic helix 111 181 3
PS51477 Prosite Profiles IPR003822 Paired amphipathic helix 252 322 3
PTHR12346 HMMPANTHER 100 1140 3
PTHR12346:SF0 HMMPANTHER 100 1140 3
1g1eB00 Gene3D IPR003822 Paired amphipathic helix 108 180 3
1g1eB00 Gene3D IPR003822 Paired amphipathic helix 249 321 3
SSF47762 SuperFamily IPR003822 Paired amphipathic helix 250 321 3
SSF47762 SuperFamily IPR003822 Paired amphipathic helix 108 180 3
Coil ncoils Rabaptin coiled-coil domain 584 605 971

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001382SIN3 family co-repressorTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001382

Protein Properties

Ave. residue weight 115.15 Da
Charge 4.50
Isoelectric point 6.82
Molecular weight 132.88 kDa
Number of residues 1154
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS34
present during mitotic M phaseS32
present during mitotic M phaseS36
S32, S336
present during mitotic M phaseS329
present during mitotic M phaseS38
present during mitotic M phaseS336
level fluctuates during mitotic cell cycleS325
Annotation ExtensionEvidenceResidueReference
experimental evidence S32 PMID:24763107
present during mitotic M phase experimental evidence S32 PMID:21712547
present during mitotic M phase experimental evidence S34 PMID:21712547
present during mitotic M phase experimental evidence S36 PMID:21712547
present during mitotic M phase experimental evidence S38 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence S325 PMID:24763107
present during mitotic M phase experimental evidence S329 PMID:21712547
experimental evidence S336 PMID:24763107
present during mitotic M phase experimental evidence S336 PMID:21712547
O-phospho-L-threonine 693
level fluctuates during mitotic cell cycleT330
present during mitotic M phaseT42
present during mitotic M phaseT330
present during mitotic M phaseT326
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T42 PMID:21712547
present during mitotic M phase experimental evidence T326 PMID:21712547
present during mitotic M phase experimental evidence T330 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence T330 PMID:24763107
phosphorylated residue 1922
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3189during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3210during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3370during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3095during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3570during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1064.8during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
682.18during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.76during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
nts1Schizosaccharomyces specific protein Affinity Capture-MSPMID:25002536
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
External References
Database Identifier Description
NBRP SPBC1734.16c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1734.16c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1734.16c BioGRID Interaction Datasets
Expression Viewer SPBC1734.16c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1734.16c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1734.16c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1734.16c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1734.16c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1734.16c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1734.16c Cell Cycle Data
GEO SPBC1734.16c GEO profiles
PInt SPBC1734.16c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1734.16c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1734.16c Fission yeast phenotypic data & analysis
SPD / RIKEN30/30E08Orfeome Localization Data
UniProtKB/SwissProtO74755Paired amphipathic helix protein pst3
ModBaseO74755Database of comparative protein structure models
STRINGO74755Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595433SIN3 family co-repressor Pst3
RefSeq mRNANM_001021341972h- SIN3 family co-repressor Pst3 (pst3), mRNA
European Nucleotide ArchiveCAA21310.1ENA Protein Mapping
UniParcUPI00000697CDUniProt Archive

Literature for pst3

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015