pst3 (SPBC1734.16c)


Gene Standard Namepst3 Characterisation Statuspublished
Systematic IDSPBC1734.16c Feature Typeprotein coding
Synonyms Name Description
ProductSIN3 family co-repressor Pst3 Product Size1154aa, 132.88 kDa
Genomic Location Chromosome II, 1094818-1089163 (5656nt); CDS:1094653-1091098 (3556nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
chromatin remodeling155
Annotation ExtensionEvidenceWith/FromReference
chromatin silencing110
Annotation ExtensionEvidenceWith/FromReference
double-strand break repair via nonhomologous end joining7
Annotation ExtensionEvidenceWith/FromReference
histone deacetylation26
Annotation ExtensionEvidenceWith/FromReference
regulation of transcription, DNA-templated389
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Clr6 histone deacetylase complex I''12
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
nuclear chromatin359
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
Rpd3L-Expanded complex15
Annotation ExtensionEvidenceWith/FromReference
Rpd3S complex16
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationpst3Δ1451

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cyclepst3Δ877
inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cellspst3Δ88
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1094818..1094608, 1094516..1089163
Intron1094607..1094517
mRNA1094818..1089163
5' UTR1094818..1094654PMID:21511999
CDS1094653..1094608, 1094516..1091098
3' UTR1091097..1089163PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02671 Pfam IPR003822 Paired amphipathic helix 275 319 2
PF02671 Pfam IPR003822 Paired amphipathic helix 134 178 2
PF16879 Pfam IPR031693 Sin3, C-terminal 846 1096 3
PF08295 Pfam IPR013194 Histone deacetylase interacting domain 498 580 3
SM00761 SMART IPR013194 Histone deacetylase interacting domain 492 592 3
PS51477 Prosite Profiles IPR003822 Paired amphipathic helix 403 472 3
PS51477 Prosite Profiles IPR003822 Paired amphipathic helix 111 181 3
PS51477 Prosite Profiles IPR003822 Paired amphipathic helix 252 322 3
PTHR12346:SF0 HMMPANTHER 95 1140 3
PTHR12346 HMMPANTHER 95 1140 3
1g1eB00 Gene3D IPR003822 Paired amphipathic helix 249 321 3
1g1eB00 Gene3D IPR003822 Paired amphipathic helix 108 180 3
SSF47762 SuperFamily IPR003822 Paired amphipathic helix 250 321 3
SSF47762 SuperFamily IPR003822 Paired amphipathic helix 108 180 3
Coil ncoils Predicted coiled-coil protein (DUF2205) 584 604 1057

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001382SIN3 family co-repressorTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001382

Protein Properties

Ave. residue weight 115.15 Da
Charge 4.50
Codon Adaptation Index 0.39
Isoelectric point 6.82
Molecular weight 132.88 kDa
Number of residues 1154
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS38
present during mitotic M phaseS336
present during mitotic M phaseS36
present during mitotic M phaseS32
present during mitotic M phaseS34
present during mitotic M phaseS329
S32, S336
level fluctuates during mitotic cell cycleS325
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S38 PMID:21712547
present during mitotic M phase experimental evidence S336 PMID:21712547
present during mitotic M phase experimental evidence S36 PMID:21712547
present during mitotic M phase experimental evidence S32 PMID:21712547
present during mitotic M phase experimental evidence S34 PMID:21712547
present during mitotic M phase experimental evidence S329 PMID:21712547
experimental evidence S32 PMID:24763107
experimental evidence S336 PMID:24763107
level fluctuates during mitotic cell cycle experimental evidence S325 PMID:24763107
O-phospho-L-threonine 701
present during mitotic M phaseT42
present during mitotic M phaseT330
present during mitotic M phaseT326
level fluctuates during mitotic cell cycleT330
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T42 PMID:21712547
present during mitotic M phase experimental evidence T330 PMID:21712547
present during mitotic M phase experimental evidence T326 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence T330 PMID:24763107
phosphorylated residue 1931
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pst3 (SPBC1734.16c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3189during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3210during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3370during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3095during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1064.8during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3570during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
682.18during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.76during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2506
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC1734.16c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured bynts1Clr6 histone deacetylase complex subunit Nts1 Affinity Capture-MSPMID:25002536
External References
Database Identifier Description
NBRP SPBC1734.16c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1734.16c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1734.16c BioGRID Interaction Datasets
Expression Viewer SPBC1734.16c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1734.16c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1734.16c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1734.16c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1734.16c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1734.16c Transcriptome Viewer (Bähler Lab)
GEO SPBC1734.16c GEO profiles
PInt SPBC1734.16c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1734.16c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1734.16c Fission yeast phenotypic data & analysis
Cyclebase SPBC1734.16c.1 Cell Cycle Data
SPD / RIKEN30/30E08Orfeome Localization Data
UniProtKB/SwissProtO74755Paired amphipathic helix protein pst3
ModBaseO74755Database of comparative protein structure models
STRINGO74755Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595433SIN3 family co-repressor Pst3
RefSeq mRNANM_001021341972h- SIN3 family co-repressor Pst3 (pst3), mRNA

Literature for pst3

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016