ppk21 (SPBC1778.10c)


Gene Standard Nameppk21 Characterisation Statuspublished
Systematic IDSPBC1778.10c Feature Typeprotein coding
SynonymsSPBC4C3.11 Name Description
Productserine/threonine protein kinase Ppk21 Product Size551aa, 62.72 kDa
Genomic Location Chromosome II, 3116630-3114231 (2400nt); CDS:3116386-3114461 (1926nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
protein serine/threonine kinase activity105
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

signaling


Term NameCount
protein phosphorylation130
Annotation ExtensionEvidenceWith/FromReference
signal transduction365
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site313
Annotation ExtensionEvidenceWith/FromReference
cell tip204
Annotation ExtensionEvidenceWith/FromReference
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body218
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
sensitive to bortezomibppk21Δ255
viable vegetative cell populationppk21Δ3837

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologyppk21Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3116630..3116183, 3116079..3115843, 3115788..3115679, 3115615..3115445, 3115394..3114231
Intron3116182..3116080, 3115842..3115789, 3115678..3115616, 3115444..3115395
5' UTR3116630..3116387PMID:21511999
3' UTR3114460..3114231PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 56 315 108
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 55 315 107
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 175 187 97
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 61 93 73
PS50011 Prosite Profiles IPR000719 Protein kinase domain 55 315 110
PTHR24356 HMMPANTHER 50 329 13
PTHR24356 HMMPANTHER 421 534 13
PTHR24356 HMMPANTHER 349 391 13
PTHR24356:SF163 HMMPANTHER 50 329 2
PTHR24356:SF163 HMMPANTHER 421 534 2
PTHR24356:SF163 HMMPANTHER 349 391 2
1.10.510.10 Gene3D AGC-kinase, C-terminal 165 314 112
2.30.29.30 Gene3D IPR011993 PH domain-like 446 539 38
2.30.29.30 Gene3D IPR011993 PH domain-like 315 329 38
2.30.29.30 Gene3D IPR011993 PH domain-like 390 391 38
3.30.200.20 Gene3D AGC-kinase, C-terminal 15 164 111
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 54 399 127

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0004366PKC subfamilyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0004366

Protein Properties

Ave. residue weight 113.82 Da
Charge 9.50
Codon Adaptation Index 0.43
Isoelectric point 8.38
Molecular weight 62.72 kDa
Number of residues 551
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS541
present during mitotic M phaseS538
present during mitotic M phaseS540
S534, S535, S538
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S541 PMID:21712547
present during mitotic M phase experimental evidence S538 PMID:21712547
present during mitotic M phase experimental evidence S540 PMID:21712547
experimental evidence S534 PMID:24763107
experimental evidence S535 PMID:24763107
experimental evidence S538 PMID:24763107
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for ppk21 (SPBC1778.10c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3594during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3559.93during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.1during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.64during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999433
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC1778.10c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withhtr11serine protease (predicted) Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC1778.10c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withmso1endocytic docking protein Mso1 Negative GeneticPMID:22681890
negative genetic interaction withSPBC1289.14adducin (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmak10NatC N-acetyltransferase complex subunit Mak10 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withppr8mitochondrial PPR repeat protein Ppr8 Negative GeneticPMID:22681890
negative genetic interaction withdef1RNAPII degradation factor Def1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC1805.14Schizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withmdl1mitochondrial peptide-transporting ATPase Negative GeneticPMID:22681890
negative genetic interaction withSPAC10F6.04RCC domain protein Ats1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withslt1Schizosaccharomyces specific protein Slt1 Negative GeneticPMID:22681890
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
negative genetic interaction withset3histone lysine methyltransferase Set3 Negative GeneticPMID:22681890
negative genetic interaction withtma20RNA-binding protein Tma20 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withatb2tubulin alpha 2 Negative GeneticPMID:22681890
negative genetic interaction withrpl380160S ribosomal protein L38 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withsgf29SAGA complex subunit Sgf29 Negative GeneticPMID:22681890
negative genetic interaction withknk1AAA family ATPase kink, Knk1 Negative GeneticPMID:22681890
negative genetic interaction withrok1ATP-dependent RNA helicase Rok1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withany2arrestin-related endocytic adaptor Any2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withapl6AP-3 adaptor complex subunit Apl6 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC24B11.08cCOPII-coated vesicle component Erv46 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcdb4curved DNA-binding protein Cdb4, peptidase family Negative GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc4 Negative GeneticPMID:21504829
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withies4Ino80 complex subunit Ies4 Negative GeneticPMID:22681890
negative genetic interaction withSPBC56F2.03actin-like protein Arp10 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withswc2Swr1 complex complex subunit Swc2 Negative GeneticPMID:22681890
negative genetic interaction withash2Ash2-trithorax family protein Negative GeneticPMID:22681890
negative genetic interaction witharf6ADP-ribosylation factor, Arf family Arf6 Negative GeneticPMID:22681890
negative genetic interaction withmcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:22681890
negative genetic interaction withSPAC186.04cN-terminal of transmembrane channel, truncated Negative GeneticPMID:22681890
negative genetic interaction withpun1SUR7 family protein Pun1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgim1prefoldin subunit 6, Gim1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsum2translation initiation inhibitor (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC1347.09hexaprenyldihydroxybenzoate methyltransferase, Coq3 variant (predicted) Negative GeneticPMID:22681890
negative genetic interaction withspn1mitotic septin Spn1 Negative GeneticPMID:22681890
negative genetic interaction withrpp20360S acidic ribosomal protein A2 Negative GeneticPMID:22681890
negative genetic interaction withSPAC29B12.11chuman WW domain binding protein-2 ortholog Negative GeneticPMID:22681890
negative genetic interaction withdao1D-amino acid oxidase Negative GeneticPMID:22681890
negative genetic interaction withatl1alkyltransferase-like protein Atl1 Negative GeneticPMID:22681890
negative genetic interaction withSPAC15A10.06CPA1 sodium ion/proton antiporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction withalg5dolichyl-phosphate beta-glucosyltransferase Alg5 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withfmd2glutathione-dependent formaldehyde dehydrogenase Fmd2 Positive GeneticPMID:22681890
positive genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPAC11G7.01serine-rich Schizosaccharomyces specific protein Positive GeneticPMID:22681890
positive genetic interaction withelp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPAP8A3.07cphospho-2-dehydro-3-deoxyheptonate aldolase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withbcs1mitochondrial Rieske ISP assembly ATPase Bcs1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withfus1formin Fus1 Positive GeneticPMID:22681890
synthetic lethal withcia1histone chaperone Cia1 Synthetic LethalityPMID:22291963
External References
Database Identifier Description
NBRP SPBC1778.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1778.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1778.10c BioGRID Interaction Datasets
Expression Viewer SPBC1778.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1778.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1778.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1778.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1778.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1778.10c Transcriptome Viewer (Bähler Lab)
GEO SPBC1778.10c GEO profiles
PInt SPBC1778.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1778.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1778.10c Fission yeast phenotypic data & analysis
Cyclebase SPBC1778.10c.1 Cell Cycle Data
SPD / RIKEN26/26G10Orfeome Localization Data
UniProtKB/SwissProtQ9Y7J6Serine/threonine-protein kinase ppk21
ModBaseQ9Y7J6Database of comparative protein structure models
STRINGQ9Y7J6Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001713090972h- serine/threonine protein kinase Ppk21 (ppk21), mRNA
RefSeq PeptideXP_001713142serine/threonine protein kinase Ppk21

Literature for ppk21

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016