ppk21 (SPBC1778.10c)


Gene Standard Nameppk21 Characterisation Statuspublished
Systematic IDSPBC1778.10c Feature Typeprotein coding
SynonymsSPBC4C3.11 Name Description
Productserine/threonine protein kinase Ppk21 Product Size551aa, 62.72 kDa
Genomic Location Chromosome II, 3116630-3114231 (2400nt); CDS:3116386-3114461 (1926nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0004674protein serine/threonine kinase activityIEAIPR008271GO_REF:0000002112
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000281mitotic cytokinesisTASPMID:1582113982
GO:0006468protein phosphorylationISMPFAM:PF00069GO_REF:000000147
GO:0007165signal transductionNASGO_REF:0000051306
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032153cell division siteIDAPMID:16823372298
GO:0051286cell tipIDAPMID:16823372192
GO:0005829cytosolIDAPMID:168233722319
GO:0044732mitotic spindle pole bodyIDAPMID:16823372214
GO:0005634nucleusIDAPMID:168233722737
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001701sensitive to bortezomib256
expressivity FYPO_EXT:0000002Cell growth assayppk21ΔNullPECO:0000005, PECO:0000137PMID:21760946
FYPO:0002060viable vegetative cell populationMicroscopyppk21ΔNullPECO:0000005, PECO:0000137PMID:236978063751
Microscopyppk21ΔNullPMID:15821139
Microscopyppk21ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopyppk21ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
131166303116183
231160793115843
331157883115679
431156153115445
531153943114231
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 56 315 109
SM00220 SMART IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain 55 315 107
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 61 93 73
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 175 187 97
PS50011 Prosite Profiles IPR000719 Protein kinase domain 55 315 110
PTHR24352:SF0 HMMPANTHER 9 550 2
PTHR24352 HMMPANTHER 9 550 4
2.30.29.30 Gene3D IPR011993 Pleckstrin homology-like domain 390 391 38
2.30.29.30 Gene3D IPR011993 Pleckstrin homology-like domain 446 539 38
2.30.29.30 Gene3D IPR011993 Pleckstrin homology-like domain 315 329 38
3.30.200.20 Gene3D 15 164 111
1.10.510.10 Gene3D 165 314 112
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 54 399 127

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0004366PKC subfamilyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0004366

Protein Properties

Ave. residue weight 113.82 Da
Charge 9.50
Isoelectric point 8.38
Molecular weight 62.72 kDa
Number of residues 551
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS541PMID:217125471670
present during mitotic M phase
S534PMID:24763107
S538PMID:24763107
S535PMID:24763107
present during mitotic M phaseS538PMID:21712547
present during mitotic M phaseS540PMID:21712547
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3594during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
3559.93during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.1during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.64during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999432
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
pun1SUR7 family protein Pun1 (predicted) Negative GeneticPMID:22681890
SPAP8A3.07cphospho-2-dehydro-3-deoxyheptonate aldolase (predicted) Positive GeneticPMID:22681890
fus1formin Fus1 Positive GeneticPMID:22681890
cia1histone chaperone Cia1 Synthetic LethalityPMID:22291963
cdb4curved DNA-binding protein Cdb4, peptidase family Negative GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
spn1septin Spn1 Negative GeneticPMID:22681890
mso1endocytic docking protein Mso1 Negative GeneticPMID:22681890
SPAC10F6.04RCC domain protein Ats1 (predicted) Negative GeneticPMID:22681890
SPBC56F2.03actin-like protein Arp10 (predicted) Negative GeneticPMID:22681890
SPAC644.07mitochondrial Rieske ISP assembly ATPase (predicted) Positive GeneticPMID:22681890
arf6ADP-ribosylation factor, Arf family Arf6 Negative GeneticPMID:22681890
rok1ATP-dependent RNA helicase Rok1 (predicted) Negative GeneticPMID:22681890
SPBC1289.14adducin (predicted) Negative GeneticPMID:22681890
elp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:22681890
atl1alkyltransferase-like protein Atl1 Negative GeneticPMID:22681890
fmd2glutathione-dependent formaldehyde dehydrogenase (predicted) Positive GeneticPMID:22681890
SPAC1F12.05arrestin-related endocytic adaptor Any2 (predicted) Negative GeneticPMID:22681890
apl6AP-3 adaptor complex subunit Apl6 (predicted) Negative GeneticPMID:22681890
tma20RNA-binding protein Tma20 (predicted) Negative GeneticPMID:22681890
ash2Ash2-trithorax family protein Negative GeneticPMID:22681890
SPAC1805.14Schizosaccharomyces specific protein Negative GeneticPMID:22681890
rpp20360S acidic ribosomal protein A2 Negative GeneticPMID:22681890
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
dao1D-amino acid oxidase Negative GeneticPMID:22681890
SPAC24B11.08cCOPII-coated vesicle component Erv46 (predicted) Negative GeneticPMID:22681890
def1RNAPII degradation factor Def1 (predicted) Negative GeneticPMID:22681890
mcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:22681890
tom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
sgf29SAGA complex subunit Sgf29 Negative GeneticPMID:22681890
set3histone lysine methyltransferase Set3 Negative GeneticPMID:22681890
swc2Swr1 complex complex subunit Swc2 Negative GeneticPMID:22681890
mak10NatC N-acetyltransferase complex subunit Mak10 (predicted) Negative GeneticPMID:22681890
ppr8mitochondrial PPR repeat protein Ppr8 Negative GeneticPMID:22681890
mdl1mitochondrial peptide-transporting ATPase Negative GeneticPMID:22681890
atb2tubulin alpha 2 Negative GeneticPMID:22681890
SPAC186.04cN-terminal of transmembrane channel, truncated Negative GeneticPMID:22681890
dad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
alg5dolichyl-phosphate beta-glucosyltransferase Alg5 (predicted) Negative GeneticPMID:22681890
SPAC29B12.11chuman WW domain binding protein-2 ortholog Negative GeneticPMID:22681890
slt1Schizosaccharomyces specific protein Slt1 Negative GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
SPBC1347.09hexaprenyldihydroxybenzoate methyltransferase, Coq3 variant (predicted) Negative GeneticPMID:22681890
ies4Ino80 complex subunit Ies4 Negative GeneticPMID:22681890
rpl380160S ribosomal protein L38 (predicted) Negative GeneticPMID:22681890
alf1ATP-dependent microtubule severing protein (predicted) Negative GeneticPMID:22681890
SPAC3A11.13prefoldin subunit 6 (predicted) Negative GeneticPMID:22681890
SPAC15A10.06CPA1 sodium ion/proton antiporter (predicted) Negative GeneticPMID:22681890
SPAC11G7.01serine-rich Schizosaccharomyces specific protein Positive GeneticPMID:22681890
sum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPBC1778.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1778.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1778.10c BioGRID Interaction Datasets
Expression Viewer SPBC1778.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1778.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1778.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1778.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1778.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1778.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1778.10c Cell Cycle Data
GEO SPBC1778.10c GEO profiles
PInt SPBC1778.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1778.10c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN26/26G10Orfeome Localization Data
UniProtKB/SwissProtQ9Y7J6Serine/threonine-protein kinase ppk21
ModBaseQ9Y7J6Database of comparative protein structure models
STRINGQ9Y7J6Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001713090972h- serine/threonine protein kinase Ppk21 (ppk21), mRNA
RefSeq PeptideXP_001713142serine/threonine protein kinase Ppk21
European Nucleotide ArchiveCAB39805.3ENA Protein Mapping
UniParcUPI000228F3ACUniProt Archive

Literature for ppk21

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014