pyr1 (SPBC17G9.11c)

Gene Standard Namepyr1 Characterisation Statuspublished
Systematic IDSPBC17G9.11c Feature Typeprotein coding
Synonyms Name Description
Productpyruvate carboxylase Pyr1 Product Size1185aa, 130.86 kDa
Genomic Location Chromosome II, 2194621-2190645 (3977nt); CDS:2194431-2190874 (3558nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
DNA binding379
Annotation ExtensionEvidenceWith/FromReference
metal ion binding747
Annotation ExtensionEvidenceWith/FromReference
pyruvate carboxylase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
generation of precursor metabolites and energy94
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationpyr1ΔNull1428

Cell Phenotype

Term NameAlleleExpressionCount
inviable after spore germination, single cell division, normal cell morphologypyr1ΔNull8
inviable after spore germination, without cell division, with normal germ tube morphologypyr1ΔNull303
inviable sporepyr1ΔNull468
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR2194621..2194432PMID:21511999
3' UTR2190873..2190645AU013709
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02786 Pfam IPR005479 Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain 149 357 4
PF00364 Pfam IPR000089 Biotin/lipoyl attachment 1117 1181 5
PF02785 Pfam IPR005482 Biotin carboxylase, C-terminal 373 480 2
PF00682 Pfam IPR000891 Pyruvate carboxyltransferase 604 823 3
PF00289 Pfam IPR005481 Carbamoyl-phosphate synthase, large subunit, N-terminal 32 144 4
PF02436 Pfam IPR003379 Carboxylase, conserved domain 865 1065 1
SM00878 SMART IPR005482 Biotin carboxylase, C-terminal 373 480 2
PS00867 Prosite Patterns IPR005479 Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain 320 327 4
PS00188 Prosite Patterns IPR001882 Biotin-binding site 1139 1156 2
PS50975 Prosite Profiles IPR011761 ATP-grasp fold 154 351 6
PS50979 Prosite Profiles IPR011764 Biotin carboxylation domain 32 484 2
PS50968 Prosite Profiles IPR000089 Biotin/lipoyl attachment 1115 1182 5
PS50991 Prosite Profiles IPR000891 Pyruvate carboxyltransferase 570 838 3
PTHR18866:SF10 HMMPANTHER IPR005930 Pyruvate carboxylase 11 1185 1
PTHR18866 HMMPANTHER 11 1185 2 Gene3D IPR016185 Pre-ATP-grasp domain 33 163 6
2qf7A03 Gene3D 1026 1103 1 Gene3D 1108 1183 7 Gene3D IPR013785 Aldolase-type TIM barrel 547 856 35
3.30.470.20 Gene3D IPR013816 ATP-grasp fold, subdomain 2 238 485 11
3.30.1490.20 Gene3D IPR013815 ATP-grasp fold, subdomain 1 164 236 7 Gene3D IPR009057 Homeodomain-like 990 1025 24
SSF89000 SuperFamily 870 1043 2
SSF52440 SuperFamily IPR016185 Pre-ATP-grasp domain 32 147 7
SSF51230 SuperFamily IPR011053 Single hybrid motif 1104 1183 6
SSF51246 SuperFamily IPR011054 Rudiment single hybrid motif 368 486 4
SSF51569 SuperFamily 564 873 9
SSF56059 SuperFamily 117 383 11
PIRSF001594 PIRSF IPR005930 Pyruvate carboxylase 28 1184 1
TIGR01235 tigrfam IPR005930 Pyruvate carboxylase 35 1183 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.43 Da
Charge 10.00
Isoelectric point 7.10
Molecular weight 130.86 kDa
Number of residues 1185

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS863
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S863 PMID:21712547
phosphorylated residue 1926
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
biotinylated residue 2
Annotation ExtensionEvidenceResidueReference
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
153435during GO:0000080PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
166762during GO:0000084PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
161926during GO:0000085PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
152135during GO:0000087PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
158038during GO:0072690PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
55361.24during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
25202.24during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
27during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
5.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633
Disease Association
lactic acidosis6
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
conserved in metazoa3430
conserved in vertebrates3405

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPBC17G9.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC17G9.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC17G9.11c BioGRID Interaction Datasets
Expression Viewer SPBC17G9.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC17G9.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC17G9.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC17G9.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC17G9.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC17G9.11c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC17G9.11c Cell Cycle Data
GEO SPBC17G9.11c GEO profiles
PInt SPBC17G9.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC17G9.11c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC17G9.11c Fission yeast phenotypic data & analysis
IntEnz6.4.1.1Integrated relational Enzyme database
Rhea6.4.1.1Annotated reactions database
SPD / RIKEN41/41A07Orfeome Localization Data
UniProtKB/SwissProtQ9UUE1Pyruvate carboxylase
ModBaseQ9UUE1Database of comparative protein structure models
STRINGQ9UUE1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595900pyruvate carboxylase Pyr1
RefSeq mRNANM_001021807972h- pyruvate carboxylase Pyr1 (pyr1), mRNA
European Nucleotide ArchiveBAA11239.1ENA Protein Mapping
European Nucleotide ArchiveCAB52809.1ENA Protein Mapping
MetaCycPWY-5750Itaconate biosynthesis
MetaCycPWY-6142Gluconeogenesis II (Methanobacterium thermoautotrophicum)
MetaCycPWY-6146Methanobacterium thermoautotrophicum biosynthetic metabolism
KEGG_Enzyme00020+ cycle (TCA cycle)
KEGG_Enzyme00620+ metabolism
KEGG_Enzyme00720+ fixation pathways in prokaryotes
UniParcUPI000006C422UniProt Archive
UniPathwayUPA00138Carbohydrate biosynthesis; gluconeogenesis

Literature for pyr1

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015