pyr1 (SPBC17G9.11c)

Gene Standard Namepyr1 Characterisation Statuspublished
Systematic IDSPBC17G9.11c Feature Typeprotein coding
Synonyms Name Description
Productpyruvate carboxylase Pyr1 Product Size1185aa, 130.86 kDa
Genomic Location Chromosome II, 2194621-2190645 (3977nt); CDS:2194431-2190874 (3558nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding560
Annotation ExtensionEvidenceWith/FromReference
biotin carboxylase activity2
Annotation ExtensionEvidenceWith/FromReference
DNA binding367
Annotation ExtensionEvidenceWith/FromReference
metal ion binding749
Annotation ExtensionEvidenceWith/FromReference
pyruvate carboxylase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
generation of precursor metabolites and energy82
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationpyr1Δ1452

Cell Phenotype

Term NameGenotypesCount
inviable after spore germination, single cell division, normal cell morphologypyr1Δ8
inviable after spore germination, without cell division, with normal germ tube morphologypyr1Δ303
inviable sporepyr1Δ476
Target Of
GO substrate of sst2 human AMSH/STAMBP protein homolog, ubiquitin specific-protease
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
GO substrate of ubp9 ubiquitin C-terminal hydrolase Ubp9
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR2194621..2194432PMID:21511999
3' UTR2190873..2190645AU013709
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00289 Pfam IPR005481 Biotin carboxylase-like, N-terminal domain 33 144 2
PF02785 Pfam IPR005482 Biotin carboxylase, C-terminal 373 480 2
PF00364 Pfam IPR000089 Biotin/lipoyl attachment 1117 1181 5
PF00682 Pfam IPR000891 Pyruvate carboxyltransferase 572 842 3
PF02786 Pfam IPR005479 Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain 149 357 4
PF02436 Pfam IPR003379 Carboxylase, conserved domain 865 1065 1
SM00878 SMART IPR005482 Biotin carboxylase, C-terminal 373 480 2
PS00867 Prosite Patterns IPR005479 Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain 320 327 4
PS00188 Prosite Patterns IPR001882 Biotin-binding site 1139 1156 2
PS50991 Prosite Profiles IPR000891 Pyruvate carboxyltransferase 570 838 3
PS50979 Prosite Profiles IPR011764 Biotin carboxylation domain 32 484 2
PS50968 Prosite Profiles IPR000089 Biotin/lipoyl attachment 1108 1183 6
PS50975 Prosite Profiles IPR011761 ATP-grasp fold 154 351 6
PTHR18866 HMMPANTHER 31 1185 2
PTHR18866:SF110 HMMPANTHER 31 1185 1 Gene3D IPR013785 Aldolase-type TIM barrel 547 856 35 Gene3D IPR009057 Homeodomain-like 990 1025 24
3.30.1490.20 Gene3D IPR013815 ATP-grasp fold, subdomain 1 164 236 7
3.30.470.20 Gene3D IPR013816 ATP-grasp fold, subdomain 2 238 485 11
2qf7A03 Gene3D 1026 1103 1 Gene3D 2-oxo acid dehydrogenase, lipoyl-binding site 1108 1183 7 Gene3D IPR016185 Pre-ATP-grasp domain 33 163 6
SSF56059 SuperFamily 117 383 11
SSF51569 SuperFamily 564 873 9
SSF51230 SuperFamily IPR011053 Single hybrid motif 1104 1183 6
SSF52440 SuperFamily IPR016185 Pre-ATP-grasp domain 32 147 7
SSF51246 SuperFamily IPR011054 Rudiment single hybrid motif 368 486 4
SSF89000 SuperFamily 870 1043 2
PIRSF001594 PIRSF IPR005930 Pyruvate carboxylase 28 1184 1
TIGR01235 TIGRFAM IPR005930 Pyruvate carboxylase 35 1183 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.43 Da
Charge 10.00
Codon Adaptation Index 0.62
Isoelectric point 7.10
Molecular weight 130.86 kDa
Number of residues 1185

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1677
present during mitotic M phaseS863
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S863 PMID:21712547
mannosylated residue 89
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence PMID:26644575
phosphorylated residue 1932
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysineK910, K916 512
Annotation ExtensionEvidenceResidueReference
IDA PMID:26412298
mass spectrometry evidence K910 PMID:26412298
mass spectrometry evidence K916 PMID:26412298
biotinylated residue 2
Annotation ExtensionEvidenceResidueReference
ISS PB_REF:0000001
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pyr1 (SPBC17G9.11c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
153435during GO:0000080PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
166762during GO:0000084PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
161926during GO:0000085PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
152135during GO:0000087PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
158038during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
55361.24during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
25202.24during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
27during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
5.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Disease Association
mitochondrial disorders28
metabolic disorders143
pyruvate carboxylase deficiency1
Taxonomic Conservation
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in bacteria1002
conserved in metazoa3424
conserved in vertebrates3399

Manually curated orthologous groups


Orthologs in Compara

External References
Database Identifier Description
NBRP SPBC17G9.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC17G9.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC17G9.11c BioGRID Interaction Datasets
Expression Viewer SPBC17G9.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC17G9.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC17G9.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC17G9.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC17G9.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC17G9.11c Transcriptome Viewer (Bähler Lab)
GEO SPBC17G9.11c GEO profiles
PInt SPBC17G9.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC17G9.11c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC17G9.11c Fission yeast phenotypic data & analysis
Cyclebase SPBC17G9.11c.1 Cell Cycle Data
IntEnz6.4.1.1Integrated relational Enzyme database
Rhea6.4.1.1Annotated reactions database
SPD / RIKEN41/41A07Orfeome Localization Data
UniProtKB/SwissProtQ9UUE1Pyruvate carboxylase
ModBaseQ9UUE1Database of comparative protein structure models
STRINGQ9UUE1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595900pyruvate carboxylase Pyr1
RefSeq mRNANM_001021807972h- pyruvate carboxylase Pyr1 (pyr1), mRNA
European Nucleotide ArchiveBAA11239.1ENA Protein Mapping
European Nucleotide ArchiveCAB52809.1ENA Protein Mapping
MetaCycPWY-5750Itaconate biosynthesis
MetaCycPWY-6142Gluconeogenesis II (Methanobacterium thermoautotrophicum)
MetaCycPWY-6146Methanobacterium thermoautotrophicum biosynthetic metabolism
KEGG00020+ cycle (TCA cycle)
KEGG00620+ metabolism
KEGG00720+ fixation pathways in prokaryotes
UniParcUPI000006C422UniProt Archive
UniPathwayUPA00138Carbohydrate biosynthesis; gluconeogenesis

Literature for pyr1

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016