pyr1 (SPBC17G9.11c)


Gene Standard Namepyr1 Characterisation Statuspublished
Systematic IDSPBC17G9.11c Feature Typeprotein coding
Synonyms Name Description
Productpyruvate carboxylase Pyr1 Product Size1185aa, 130.86 kDa
Genomic Location Chromosome II, 2194621-2190645 (3977nt); CDS:2194431-2190874 (3558nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0003677DNA bindingIEAIPR009057GO_REF:0000002385
GO:0004075biotin carboxylase activityIEAIPR005930GO_REF:00000022
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037748
GO:0004736pyruvate carboxylase activityISOSGD:S000003030GO_REF:00000241
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006091generation of precursor metabolites and energyNASGO_REF:0000051110
GO:0006094gluconeogenesisISOSGD:S000003030GO_REF:00000247
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722319
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopypyr1ΔNullPECO:0000005, PECO:0000137PMID:236978061337
Microscopypyr1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000320inviable after spore germination, single cell division, normal cell morphology8
expressivity FYPO_EXT:0000001Microscopypyr1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0000310inviable after spore germination, without cell division, with normal germ tube morphologyMicroscopypyr1ΔNullPECO:0000005, PECO:0000137PMID:23697806303
FYPO:0002151inviable sporeMicroscopypyr1ΔNullPECO:0000005, PECO:0000137PMID:23697806462
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
121946212190645
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00364 Pfam IPR000089 Biotin/lipoyl attachment 1117 1181 5
PF02786 Pfam IPR005479 Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain 149 357 4
PF02785 Pfam IPR005482 Biotin carboxylase, C-terminal 373 480 2
PF00289 Pfam IPR005481 Carbamoyl-phosphate synthase, large subunit, N-terminal 32 144 4
PF00682 Pfam IPR000891 Pyruvate carboxyltransferase 604 823 3
PF02436 Pfam IPR003379 Carboxylase, conserved domain 865 1065 1
SM00878 SMART IPR005482 Biotin carboxylase, C-terminal 373 480 2
PS50968 Prosite Profiles IPR000089 Biotin/lipoyl attachment 1115 1182 5
PS50979 Prosite Profiles IPR011764 Biotin carboxylation domain 32 484 2
PS50991 Prosite Profiles IPR000891 Pyruvate carboxyltransferase 570 838 3
PS50975 Prosite Profiles IPR011761 ATP-grasp fold 154 351 6
PS00188 Prosite Patterns IPR001882 Biotin-binding site 1139 1156 2
PS00867 Prosite Patterns IPR005479 Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain 320 327 4
PTHR18866:SF10 HMMPANTHER IPR005930 Pyruvate carboxylase 31 1185 1
PTHR18866 HMMPANTHER 31 1185 2
2.40.50.100 Gene3D 1108 1183 7
1.10.10.60 Gene3D IPR009057 Homeodomain-like 990 1025 24
3.20.20.70 Gene3D IPR013785 Aldolase-type TIM barrel 547 856 35
3.30.470.20 Gene3D IPR013816 ATP-grasp fold, subdomain 2 238 485 11
3.30.1490.20 Gene3D IPR013815 ATP-grasp fold, subdomain 1 164 236 7
2qf7A03 Gene3D 1026 1103 1
3.40.50.20 Gene3D IPR016185 Pre-ATP-grasp domain 33 163 6
SSF52440 SuperFamily IPR016185 Pre-ATP-grasp domain 32 147 7
SSF51569 SuperFamily 564 873 9
SSF51230 SuperFamily IPR011053 Single hybrid motif 1104 1183 6
SSF56059 SuperFamily 117 383 11
SSF89000 SuperFamily 870 1043 2
SSF51246 SuperFamily IPR011054 Rudiment single hybrid motif 368 486 4
PIRSF001594 PIRSF IPR005930 Pyruvate carboxylase 27 1184 1
TIGR01235 tigrfam IPR005930 Pyruvate carboxylase 35 1183 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.43 Da
Charge 10.00
Isoelectric point 7.10
Molecular weight 130.86 kDa
Number of residues 1185
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS863PMID:217125471670
present during mitotic M phase
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
153435during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
166762during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
161926during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
152135during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
158038during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
55361.24during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
25202.24during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
27during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
5.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
lactic acidosis6
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in bacteria1002
conserved in metazoa3428
conserved in vertebrates3402
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPBC17G9.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC17G9.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC17G9.11c BioGRID Interaction Datasets
Expression Viewer SPBC17G9.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC17G9.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC17G9.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC17G9.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC17G9.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC17G9.11c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC17G9.11c Cell Cycle Data
GEO SPBC17G9.11c GEO profiles
PInt SPBC17G9.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC17G9.11c Peptides identified in tandem mass spectrometry proteomics experiments
IntEnz6.4.1.1Integrated relational Enzyme database
Rhea6.4.1.1Annotated reactions database
SPD / RIKEN41/41A07Orfeome Localization Data
UniProtKB/SwissProtQ9UUE1Pyruvate carboxylase
ModBaseQ9UUE1Database of comparative protein structure models
STRINGQ9UUE1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595900pyruvate carboxylase Pyr1
RefSeq mRNANM_001021807972h- pyruvate carboxylase Pyr1 (pyr1), mRNA
European Nucleotide ArchiveBAA11239.1ENA Protein Mapping
European Nucleotide ArchiveCAB52809.1ENA Protein Mapping
MetaCycPWY-5750Itaconate biosynthesis
MetaCycPWY-6142Gluconeogenesis II (Methanobacterium thermoautotrophicum)
MetaCycPWY-6146Methanobacterium thermoautotrophicum biosynthetic metabolism
UniParcUPI000006C422UniProt Archive
UniPathwayUPA00138Carbohydrate biosynthesis; gluconeogenesis

Literature for pyr1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014