lcf1 (SPBC18H10.02)

Gene Standard Namelcf1 Characterisation Statuspublished
Systematic IDSPBC18H10.02 Feature Typeprotein coding
Synonyms Name Description
Productlong-chain-fatty-acid-CoA ligase Lcf1 Product Size676aa, 75.97 kDa
Genomic Location Chromosome II, 1769946-1772629 (2684nt); CDS:1770093-1772249 (2157nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0090432myristoyl-CoA ligase activityIMPPMID:180712492
GO:0090434oleoyl-CoA ligase activityIMPPMID:180712491
GO:0090433palmitoyl-CoA ligase activityIMPPMID:180712491
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0001676long-chain fatty acid metabolic processIMPPMID:180712495
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005783endoplasmic reticulumIDAPMID:16823372594
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000245loss of viability in stationary phaseCell growth assaylcf1ΔNullPECO:0000104PMID:1807124918
Cell growth assaylcf1ΔNullPECO:0000126PMID:18071249
FYPO:0001245sensitive to cobaltCell growth assaylcf1ΔNullPECO:0000201, PECO:0000005PMID:2280634456
expressivity FYPO_EXT:0000001
FYPO:0002060viable vegetative cell populationMicroscopylcf1ΔNullPMID:204732893755
Microscopylcf1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001304decreased myristoyl-CoA ligase activityEnzyme assay datalcf1ΔNullPMID:180712492
FYPO:0001302decreased oleoyl-CoA ligase activityEnzyme assay datalcf1ΔNullPMID:180712491
Enzyme assay datalcf1ΔNullPMID:18071249
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopylcf1ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Start End Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00501 Pfam IPR000873 AMP-dependent synthetase/ligase 92 540 9
PS00455 Prosite Patterns IPR020845 AMP-binding, conserved site 244 255 7
PTHR24096 HMMPANTHER 2 676 2
PTHR24096:SF46 HMMPANTHER 2 676 2
G3DSA: Gene3D 455 530 7
G3DSA: Gene3D 95 250 9
G3DSA: Gene3D 37 60 9
G3DSA: Gene3D 251 349 9
G3DSA: Gene3D 393 447 9
SSF56801 SuperFamily 89 354 9
SSF56801 SuperFamily 19 60 9
SSF56801 SuperFamily 396 674 9
Low complexity (SEG) seg 228 240

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.38 Da
Charge 14.00
Isoelectric point 8.33
Molecular weight 75.97 kDa
Number of residues 676
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
23445.64during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
33181.56during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
19during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Disease Association
Species Distribution
conserved in fungi4564
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3394

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
lcf2long-chain-fatty-acid-CoA ligase Phenotypic EnhancementPMID:18071249
Synthetic Growth Defect
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPBC18H10.02 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC18H10.02 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC18H10.02 BioGRID Interaction Datasets
Expression Viewer SPBC18H10.02 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC18H10.02 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC18H10.02 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC18H10.02 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC18H10.02 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC18H10.02 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC18H10.02 Cell Cycle Data
GEO SPBC18H10.02 GEO profiles
PInt SPBC18H10.02 Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2540731long-chain-fatty-acid-CoA ligase Lcf1
EntrezGene2540731long-chain-fatty-acid-CoA ligase Lcf1
IntEnz6.2.1.3Integrated relational Enzyme database
Rhea6.2.1.3Annotated reactions database
SPD / RIKEN37/37F06Orfeome Localization Data
UniProtKB/SwissProtO60135Long-chain-fatty-acid--CoA ligase 1
ModBaseO60135Database of comparative protein structure models
StringO60135Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595726long-chain-fatty-acid-CoA ligase Lcf1
RefSeq mRNANM_001021624972h- long-chain-fatty-acid-CoA ligase Lcf1 (lcf1), mRNA
European Nucleotide ArchiveCAA18399ENA Protein Mapping
UniParcUPI000006C32FUniProt Archive

Literature for lcf1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014