lcf1 (SPBC18H10.02)


Gene Standard Namelcf1 Characterisation Statuspublished
Systematic IDSPBC18H10.02 Feature Typeprotein coding
Synonyms Name Description
Productlong-chain-fatty-acid-CoA ligase Lcf1 Product Size676aa, 75.97 kDa
Genomic Location Chromosome II, 1769946-1772629 (2684nt); CDS:1770093-1772249 (2157nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0090432myristoyl-CoA ligase activityIMPPMID:180712492
GO:0090434oleoyl-CoA ligase activityIMPPMID:180712491
GO:0090433palmitoyl-CoA ligase activityIMPPMID:180712491
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0001676long-chain fatty acid metabolic processIMPPMID:180712495
redundant_withIGIlcf2PMID:18071249
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISOSGD:S000004860GO_REF:00000244204
GO:0005783endoplasmic reticulumIDAPMID:16823372594
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000245loss of viability in stationary phaseCell growth assaylcf1ΔNullPECO:0000126PMID:1807124927
Cell growth assaylcf1ΔNullPECO:0000104PMID:18071249
FYPO:0001245sensitive to cobalt56
expressivity FYPO_EXT:0000001Cell growth assaylcf1ΔNullPECO:0000201, PECO:0000005PMID:22806344
FYPO:0003559sensitive to doxorubicin91
expressivity FYPO_EXT:0000002Cell growth assaylcf1ΔNullPECO:0000137, PECO:0000142PMID:23365689
FYPO:0002060viable vegetative cell populationMicroscopylcf1ΔNullPMID:204732893751
Microscopylcf1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001304decreased myristoyl-CoA ligase activityEnzyme assay datalcf1ΔNullPMID:180712492
FYPO:0001302decreased oleoyl-CoA ligase activityEnzyme assay datalcf1ΔNullPMID:180712491
Enzyme assay datalcf1ΔNullPMID:18071249
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopylcf1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
117699461770111
217702381772629
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00501 Pfam IPR000873 AMP-dependent synthetase/ligase 92 540 9
PS00455 Prosite Patterns IPR020845 AMP-binding, conserved site 244 255 7
PTHR24096 HMMPANTHER 2 676 2
PTHR24096:SF46 HMMPANTHER 2 676 2
3.40.50.980 Gene3D 251 349 9
3.40.50.980 Gene3D 393 447 9
3.40.50.980 Gene3D 95 250 9
3.40.50.980 Gene3D 37 60 9
2.30.38.10 Gene3D 455 530 7
SSF56801 SuperFamily 19 60 9
SSF56801 SuperFamily 396 674 9
SSF56801 SuperFamily 89 354 9

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.38 Da
Charge 14.00
Isoelectric point 8.33
Molecular weight 75.97 kDa
Number of residues 676
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
55966during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
62967during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
57752during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
56774during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
58580during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
33181.56during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
23445.64during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
19during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancer142
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4599
conserved in eukaryotes4514
conserved in bacteria1002
conserved in metazoa3428
conserved in vertebrates3402
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
lcf2long-chain-fatty-acid-CoA ligase Phenotypic EnhancementPMID:18071249
Synthetic Growth Defect
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPBC18H10.02 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC18H10.02 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC18H10.02 BioGRID Interaction Datasets
Expression Viewer SPBC18H10.02 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC18H10.02 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC18H10.02 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC18H10.02 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC18H10.02 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC18H10.02 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC18H10.02 Cell Cycle Data
GEO SPBC18H10.02 GEO profiles
PInt SPBC18H10.02 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC18H10.02 Peptides identified in tandem mass spectrometry proteomics experiments
IntEnz6.2.1.3Integrated relational Enzyme database
Rhea6.2.1.3Annotated reactions database
SPD / RIKEN37/37F06Orfeome Localization Data
UniProtKB/SwissProtO60135Long-chain-fatty-acid--CoA ligase 1
ModBaseO60135Database of comparative protein structure models
STRINGO60135Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595726long-chain-fatty-acid-CoA ligase Lcf1
RefSeq mRNANM_001021624972h- long-chain-fatty-acid-CoA ligase Lcf1 (lcf1), mRNA
European Nucleotide ArchiveCAA18399.1ENA Protein Mapping
UniParcUPI000006C32FUniProt Archive

Literature for lcf1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014