mex67 (SPBC1921.03c)


Gene Standard Namemex67 Characterisation Statuspublished
Systematic IDSPBC1921.03c Feature Typeprotein coding
Synonyms Name Description
ProductmRNA export receptor, Tap, nucleoporin Mex67 Product Size596aa, 66.53 kDa
Genomic Location Chromosome II, 2410181-2408152 (2030nt); CDS:2410094-2408304 (1791nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding863
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
poly(A)+ mRNA export from nucleus17
Annotation ExtensionEvidenceWith/FromReference
ribosomal large subunit export from nucleus8
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle269
Annotation ExtensionEvidenceWith/FromReference
nuclear membrane33
Annotation ExtensionEvidenceWith/FromReference
nuclear pore51
Annotation ExtensionEvidenceWith/FromReference
nuclear RNA export factor complex2
Annotation ExtensionEvidenceWith/FromReference
nucleolus366
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationmex67Δ3837

Cell Phenotype

Term NameGenotypesCount
normal RNA level during vegetative growth122
affecting rec8mex67Δ
affecting mei4mex67Δ
viable vegetative cell with normal cell morphologymex67Δ3100

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
inviable vegetative cell populationrsm1Δ, mex67Δ
npp106+, SLMex3, mex67Δ
loss of viability at high temperaturemex67+, rae1-167
viable vegetative cell populationcrp79::ura4+(nt375-1953), mex67Δ
mex67+, rae1-167

Cell Phenotype

Term NameGenotypes
increased total nuclear polyadenylated mRNA level during vegetative growthrsm1Δ, mex67Δ
normal poly(A)+ mRNA export from nucleuscrp79::ura4+(nt375-1953), mex67Δ
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2410181..2408152
5' UTR2410181..2410095PMID:21511999
3' UTR2408303..2408152PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03943 Pfam IPR005637 TAP C-terminal (TAP-C) domain 547 595 1
SM00804 SMART IPR005637 TAP C-terminal (TAP-C) domain 534 596 1
PS50177 Prosite Profiles IPR018222 Nuclear transport factor 2, eukaryote 338 499 4
PS51450 Prosite Profiles IPR001611 Leucine-rich repeat 215 236 9
PS51450 Prosite Profiles IPR001611 Leucine-rich repeat 241 262 9
PS51281 Prosite Profiles IPR005637 TAP C-terminal (TAP-C) domain 543 596 1
PTHR10662:SF22 HMMPANTHER 150 595 1
PTHR10662 HMMPANTHER IPR030217 Nuclear RNA export factor 150 595 1
3.10.450.50 Gene3D IPR032710 NTF2-like domain 324 506 5
1.10.8.10 Gene3D Modification methylase HemK 543 595 19
3.80.10.10 Gene3D IPR032675 Leucine-rich repeat domain, L domain-like 154 313 16
SSF46934 SuperFamily IPR009060 UBA-like 538 595 13
SSF54427 SuperFamily IPR032710 NTF2-like domain 327 517 6
SSF52058 SuperFamily IPR032675 Leucine-rich repeat domain, L domain-like 154 313 11

View domain organization at Pfam

Term IDTerm NameReferenceCount
SO:0001531nuclear_export_signal7

Protein Properties

Ave. residue weight 111.64 Da
Charge 26.00
Codon Adaptation Index 0.38
Isoelectric point 10.46
Molecular weight 66.53 kDa
Number of residues 596
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for mex67 (SPBC1921.03c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
17043during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
16415during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
15289during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
17921during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1461.9during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
18161during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1262.67during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.59during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
heteromeric(2) PMID:1496304641
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC1921.03c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withrad51RecA family recombinase Rad51/Rhp51 Two-hybridPMID:23695164
binds DNA-binding domain construct withtoe1transcription factor, zf-fungal binuclear cluster type Two-hybridPMID:26771498
forms complex withmlo3RNA binding protein Mlo3 Reconstituted ComplexPMID:15990877
forms complex withrae1RNA export factor, nucleoporin Rae1 Reconstituted ComplexPMID:11073978
affinity captured byred5human ZC3H3 homolog Affinity Capture-MSPMID:24713849
affinity capturesrae1RNA export factor, nucleoporin Rae1 Affinity Capture-WesternPMID:11073978
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC1921.03c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withprp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
negative genetic interaction withres2MBF transcription factor complex subunit Res2 Negative GeneticPMID:22681890
negative genetic interaction withSPBC21C3.03ABC1 kinase family protein Negative GeneticPMID:22681890
negative genetic interaction withdbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
negative genetic interaction withfin1serine/threonine protein kinase, NIMA related Fin1 Negative GeneticPMID:22681890
negative genetic interaction withnot2CCR4-Not complex subunit Not2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withfkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withnot3CCR4-Not complex subunit Not3/5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPCC24B10.18human Leydig cell tumor 10 kDa protein homolog Negative GeneticPMID:22681890
negative genetic interaction withdcr1dicer Negative GeneticPMID:22681890
negative genetic interaction withcut2securin, sister chromatid separation inhibitor Negative GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
negative genetic interaction withcsn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:22681890
negative genetic interaction withSPAC19B12.11czinc finger protein, human ZNF593 ortholog Negative GeneticPMID:22681890
negative genetic interaction withusp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
negative genetic interaction withpnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:22681890
negative genetic interaction withtos4FHA domain protein Tos4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withago1argonaute Negative GeneticPMID:22681890
negative genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
negative genetic interaction withcdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
negative genetic interaction withsum2translation initiation inhibitor (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction withSPBC17A3.06phosphoprotein phosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Negative GeneticPMID:22681890
negative genetic interaction withcdr2serine/threonine protein kinase Cdr2 Negative GeneticPMID:22681890
negative genetic interaction withtea5pseudokinase Tea5 Negative GeneticPMID:22681890
negative genetic interaction withsck2serine/threonine protein kinase Sck2 Negative GeneticPMID:24463365
negative genetic interaction withsty1MAP kinase Sty1 Negative GeneticPMID:22681890
negative genetic interaction withpzh1serine/threonine protein phosphatase Pzh1 Negative GeneticPMID:22681890
negative genetic interaction withdsc5UBX domain containing protein required for Sre1 cleavage Negative GeneticPMID:22681890
negative genetic interaction withsap114U2 snRNP subunit Sap114 Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
negative genetic interaction withsdc1Dpy-30 domain protein Sdc1 Negative GeneticPMID:22681890
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
negative genetic interaction withubp6ubiquitin C-terminal hydrolase Ubp6 Negative GeneticPMID:22681890
negative genetic interaction withhrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
negative genetic interaction withpuf1pumilio family RNA-binding protein Puf1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withlub1WD repeat protein Lub1 Negative GeneticPMID:22681890
negative genetic interaction withnup132nucleoporin Nup132 Negative GeneticPMID:22681890
negative genetic interaction withtas3RITS complex subunit 3 Negative GeneticPMID:22681890
negative genetic interaction withnup40nucleoporin Nup40 Negative GeneticPMID:22681890
negative genetic interaction withrpl2260S ribosomal protein L22 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpab2poly(A) binding protein Pab2 Negative GeneticPMID:22681890
negative genetic interaction withSPAC17H9.13cglutamate 5-kinase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmug154conserved fungal protein Negative GeneticPMID:22681890
synthetic lethal withrsm1RNA export factor Rsm1 Synthetic LethalityPMID:15357289
synthetic lethal withrmn1RNA-binding protein Synthetic LethalityPMID:22936388
synthetic lethal withelf1AAA family ATPase Elf1 Synthetic LethalityPMID:12110682
synthetic lethal withnup184nucleoporin Nup184 Synthetic LethalityPMID:11073978
synthetic lethal withmlo3RNA binding protein Mlo3 Synthetic LethalityPMID:15990877
synthetic lethal withrpn15proteasome regulatory particle, lid subcomplex subunit Rpn15/Dss1 Synthetic LethalityPMID:15990877
synthetic lethal withrae1RNA export factor, nucleoporin Rae1 Synthetic LethalityPMID:11073978
synthetic lethal withnpp106nucleoporin Npp106 Synthetic LethalityPMID:11073978
positive genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
positive genetic interaction withclg1cyclin Clg1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withhrp3ATP-dependent DNA helicase Hrp3 Positive GeneticPMID:22681890
positive genetic interaction withtma23ribosome biogenesis protein Tma23 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Positive GeneticPMID:22681890
positive genetic interaction withelp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withphp3CCAAT-binding factor complex subunit Php3 Positive GeneticPMID:22681890
rescued bypst2Clr6 histone deacetylase complex subunit Pst2 Phenotypic SuppressionPMID:19547744
synthetic growth defect withred5human ZC3H3 homolog Synthetic Growth DefectPMID:23658229
overexpression rescuesrae1RNA export factor, nucleoporin Rae1 Dosage RescuePMID:12110682
overexpression rescueself1AAA family ATPase Elf1 Dosage RescuePMID:12110682
synthetically rescueself1AAA family ATPase Elf1 Synthetic RescuePMID:12110682
synthetically rescuesmad2mitotic spindle checkpoint protein Mad2 Synthetic RescuePMID:22737087
synthetically rescuesgtb1gamma-tubulin Gtb1 Synthetic RescuePMID:22737087
synthetically rescuesrae1RNA export factor, nucleoporin Rae1 Synthetic RescuePMID:11073978
rescued by overexpression ofrae1RNA export factor, nucleoporin Rae1 Dosage RescuePMID:12110682
External References
Database Identifier Description
NBRP SPBC1921.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1921.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1921.03c BioGRID Interaction Datasets
Expression Viewer SPBC1921.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1921.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1921.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1921.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1921.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1921.03c Transcriptome Viewer (Bähler Lab)
GEO SPBC1921.03c GEO profiles
PInt SPBC1921.03c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1921.03c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1921.03c Fission yeast phenotypic data & analysis
Cyclebase SPBC1921.03c.1 Cell Cycle Data
SPD / RIKEN33/33D08Orfeome Localization Data
UniProtKB/SwissProtQ9Y8G3mRNA export factor mex67
ModBaseQ9Y8G3Database of comparative protein structure models
STRINGQ9Y8G3Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595996mRNA export receptor, Tap, nucleoporin Mex67
RefSeq mRNANM_001021904972h- mRNA export receptor, Tap, nucleoporin Mex67 (mex67), mRNA

Literature for mex67

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016