mex67 (SPBC1921.03c)


Gene Standard Namemex67 Characterisation Statuspublished
Systematic IDSPBC1921.03c Feature Typeprotein coding
Synonyms Name Description
ProductmRNA export receptor, Tap, nucleoporin Mex67 Product Size596aa, 66.53 kDa
Genomic Location Chromosome II, 2410181-2408152 (2030nt); CDS:2410094-2408304 (1791nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005515protein bindingIPIrae1PMID:11073978865
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0016973poly(A)+ mRNA export from nucleusIGIrae1PMID:1107397817
GO:0000055ribosomal large subunit export from nucleusISOSGD:S000006090GO_REF:00000248
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722319
GO:0072686mitotic spindleIDAPMID:16823372254
GO:0042272nuclear RNA export factor complexIPInxt1PMID:149630462
GO:0031965nuclear membrane51
predominantlyIDAPMID:15840944
GO:0005643nuclear poreISOSGD:S000006090GO_REF:000002454
GO:0005730nucleolus357
during cellular response to stressIDAPMID:15840944
GO:0005634nucleusIDAPMID:168233722737
IDAPMID:15840944
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopymex67ΔNullPMID:204732893751
Microscopymex67ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopynot recorded (unrecorded)PMID:11073978

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001317normal RNA level during vegetative growth68
affecting rec8Transcript expression level evidencemex67ΔNullPMID:23658229
affecting mei4Transcript expression level evidencemex67ΔNullPMID:23658229
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopymex67ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
124101812408152
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03943 Pfam IPR005637 TAP C-terminal (TAP-C) domain 546 596 1
SM00804 SMART IPR005637 TAP C-terminal (TAP-C) domain 534 596 1
PS51281 Prosite Profiles IPR005637 TAP C-terminal (TAP-C) domain 543 596 1
PS50177 Prosite Profiles IPR018222 Nuclear transport factor 2, eukaryote 338 499 4
PS51450 Prosite Profiles IPR001611 Leucine-rich repeat 215 236 9
PS51450 Prosite Profiles IPR001611 Leucine-rich repeat 241 262 9
PTHR10662 HMMPANTHER 8 595 1
PTHR10662:SF22 HMMPANTHER 8 595 1
3.10.450.50 Gene3D 324 506 5
1.10.8.10 Gene3D 543 595 19
3.80.10.10 Gene3D 154 313 16
SSF54427 SuperFamily 327 517 6
SSF52058 SuperFamily 154 313 11
SSF46934 SuperFamily IPR009060 UBA-like 538 595 13

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001531nuclear_export_signalPMID:149630467

Protein Properties

Ave. residue weight 111.64 Da
Charge 26.00
Isoelectric point 10.46
Molecular weight 66.53 kDa
Number of residues 596
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
17043during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
16415during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
15289during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
17921during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1461.9during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
18161during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1262.67during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.59during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
heteromeric(2) PMID:1496304642
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
pnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:22681890
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
ase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
lub1WD repeat protein Lub1 Negative GeneticPMID:22681890
prp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
rmn1RNA-binding protein Synthetic LethalityPMID:22936388
ubp6ubiquitin C-terminal hydrolase Ubp6 Negative GeneticPMID:22681890
ago1argonaute Negative GeneticPMID:22681890
cdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Negative GeneticPMID:22681890
nup132nucleoporin Nup132 Negative GeneticPMID:22681890
cdr2serine/threonine protein kinase Cdr2 Negative GeneticPMID:22681890
SPBC56F2.08cRNA-binding protein (predicted) Negative GeneticPMID:22681890
red5human ZC3H3 homolog Synthetic Growth DefectPMID:23658229
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
SPCC24B10.18human Leydig cell tumor 10 kDa protein homolog Negative GeneticPMID:22681890
elp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:22681890
rpn15proteasome regulatory particle, lid subcomplex subunit Rpn15/Dss11 Synthetic LethalityPMID:15990877
gtb1gamma-tubulin Gtb1 Synthetic RescuePMID:22737087
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
rpl2260S ribosomal protein L22 (predicted) Negative GeneticPMID:22681890
sty1MAP kinase Sty1 Negative GeneticPMID:22681890
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
pst2Clr6 histone deacetylase complex subunit Pst2 Phenotypic SuppressionPMID:19547744
pab2poly(A) binding protein Pab2 Negative GeneticPMID:22681890
mug80cyclin Clg1 (predicted) Positive GeneticPMID:22681890
not2CCR4-Not complex subunit Not2 (predicted) Negative GeneticPMID:22681890
tma23ribosome biogenesis protein Tma23 (predicted) Positive GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
mug154conserved fungal protein Negative GeneticPMID:22681890
fin1serine/threonine protein kinase, NIMA related Fin1 Negative GeneticPMID:22681890
rsm1RNA export factor Rsm1 Synthetic LethalityPMID:15357289
sck2serine/threonine protein kinase Sck2 Negative GeneticPMID:24463365
npp106nucleoporin Npp106 Synthetic LethalityPMID:11073978
pzh1serine/threonine protein phosphatase Pzh1 Negative GeneticPMID:22681890
SPBC17A3.06phosphoprotein phosphatase (predicted) Negative GeneticPMID:22681890
not3CCR4-Not complex subunit Not3/5 (predicted) Negative GeneticPMID:22681890
nup40nucleoporin Nup40 Negative GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
ypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Positive GeneticPMID:22681890
tas3RITS complex subunit 3 Negative GeneticPMID:22681890
sdc1Dpy-30 domain protein Sdc1 Negative GeneticPMID:22681890
cut2securin, sister chromatid separation inhibitor Negative GeneticPMID:22681890
elf1AAA family ATPase Elf1 Dosage RescuePMID:12110682
Synthetic Rescue
Synthetic Lethality
csn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:22681890
hrp3ATP-dependent DNA helicase Hrp3 Positive GeneticPMID:22681890
usp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
res2MBF transcription factor complex subunit Res2 Negative GeneticPMID:22681890
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
php3CCAAT-binding factor complex subunit Php3 Positive GeneticPMID:22681890
dsc5UBX domain containing protein required for Sre1 cleavage Negative GeneticPMID:22681890
SPAC17H9.13cglutamate 5-kinase (predicted) Negative GeneticPMID:22681890
fkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Negative GeneticPMID:22681890
mad2spindle checkpoint protein Mad2 Synthetic RescuePMID:22737087
tea5pseudokinase Tea5 Negative GeneticPMID:22681890
dcr1dicer Negative GeneticPMID:22681890
tos4FHA domain protein Tos4 (predicted) Negative GeneticPMID:22681890
dbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
nup184nucleoporin Nup184 Synthetic LethalityPMID:11073978
sap114U2 snRNP subunit Sap114 Negative GeneticPMID:22681890
mlo3RNA binding protein Mlo3 Synthetic LethalityPMID:15990877
SPBC21C3.03ABC1 kinase family protein Negative GeneticPMID:22681890
SPAC19B12.11czinc finger protein, human ZNF593 ortholog Negative GeneticPMID:22681890
rae1RNA export factor, nucleoporin Rae1 Synthetic RescuePMID:11073978
Synthetic Lethality
Dosage RescuePMID:12110682
sum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rad51RecA family recombinase Rad51/Rhp51 Two-hybridPMID:23695164
mlo3RNA binding protein Mlo3 Reconstituted ComplexPMID:15990877
rae1RNA export factor, nucleoporin Rae1 Reconstituted ComplexPMID:11073978
Affinity Capture-Western
External References
Database Identifier Description
NBRP SPBC1921.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1921.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1921.03c BioGRID Interaction Datasets
Expression Viewer SPBC1921.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1921.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1921.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1921.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1921.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1921.03c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1921.03c Cell Cycle Data
GEO SPBC1921.03c GEO profiles
PInt SPBC1921.03c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1921.03c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN33/33D08Orfeome Localization Data
UniProtKB/SwissProtQ9Y8G3mRNA export factor mex67
ModBaseQ9Y8G3Database of comparative protein structure models
STRINGQ9Y8G3Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595996mRNA export receptor, Tap, nucleoporin Mex67
RefSeq mRNANM_001021904972h- mRNA export receptor, Tap, nucleoporin Mex67 (mex67), mRNA
European Nucleotide ArchiveAAD43831.1ENA Protein Mapping
European Nucleotide ArchiveBAA87120.1ENA Protein Mapping
European Nucleotide ArchiveCAB58969.1ENA Protein Mapping
UniParcUPI000012FAA5UniProt Archive

Literature for mex67

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014