sre1 (SPBC19C2.09)


Gene Standard Namesre1 Characterisation Statuspublished
Systematic IDSPBC19C2.09 Feature Typeprotein coding
Synonyms Name Description
Productsterol regulatory element binding protein, transcription factor Sre1 Product Size900aa, 98.49 kDa
Genomic Location Chromosome II, 1691270-1694691 (3422nt); CDS:1691828-1694530 (2703nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
cellular response to hypoxia7
Annotation ExtensionEvidenceWith/FromReference
negative regulation of transcription from RNA polymerase II promoter79
Annotation ExtensionEvidenceWith/FromReference
oxygen metabolic process2
Annotation ExtensionEvidenceWith/FromReference
positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of heme biosynthetic process1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of phospholipid biosynthetic process by positive regulation of transcription from RNA polymerase II promoter1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription from RNA polymerase II promoter87
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription from RNA polymerase II promoter in response to hypoxia2
regulation of transcription, start site selection2
Annotation ExtensionEvidenceWith/FromReference
SREBP signaling pathway7
Annotation ExtensionEvidenceWith/FromReference
transcription from RNA polymerase II promoter335
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
integral component of cytoplasmic side of endoplasmic reticulum membrane4
Annotation ExtensionEvidenceWith/FromReference
nucleus2740
Annotation ExtensionEvidenceWith/FromReference
SREBP-SCAP complex3
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001245sensitive to cobaltCell growth assaysre1ΔNullPMID:2372966656
Cell growth assaynot recorded (L413T)Not specifiedPMID:23729666
Cell growth assaynot recorded (G412E)Not specifiedPMID:23729666
Cell growth assaynot recorded (M410E)Not specifiedPMID:23729666
Cell growth assaynot recorded (G409E)Not specifiedPMID:23729666
FYPO:0002060viable vegetative cell populationMicroscopysre1ΔNullPMID:204732893759
Microscopynot recorded (unrecorded)PMID:15797383
Microscopysre1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001422decreased protein processing12
affecting sre1Western blot assaynot recorded (N408E)EndogenousPMID:23729666
affecting sre1Western blot assaynot recorded (G412E)EndogenousPMID:23729666
affecting sre1Western blot assaynot recorded (G409E)EndogenousPMID:23729666
affecting sre1Western blot assaynot recorded (M410E)EndogenousPMID:23729666
affecting sre1Western blot assaynot recorded (L413T)EndogenousPMID:23729666
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopysre1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in hhp2 serine/threonine protein kinase Hhp2 PMID:24327658
FYPO affected by mutation in scp1 Sre1 cleavage activating protein, Scap Scp1 PMID:18503029
FYPO affected by mutation in sre1 sterol regulatory element binding protein, transcription factor Sre1 PMID:23729666
FYPO affected by mutation in tom70 mitochondrial TOM complex subunit Tom70 (predicted) PMID:24327658
GO regulated by nro1 negative regulator of Ofd1, Nro1 PMID:22017871
GO regulated by ofd1 2-oxoglutarate and Fe(II) dioxygenase domain containing protein 1 PMID:22017871
GO regulated by scp1 Sre1 cleavage activating protein, Scap Scp1 PMID:15797383
GO regulated by sre1 sterol regulatory element binding protein, transcription factor Sre1 PMID:16537923
GO substrate of hhp2 serine/threonine protein kinase Hhp2 PMID:24327658
GO substrate of hrd1 synviolin family ubiquitin-protein ligase E3, Hrd1 PMID:19520858
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
116912701694691

UTRs

Region Coordinates Reference
five_prime_UTR1691270..1691827PMID:21511999
three_prime_UTR1694531..1694691PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00010 Pfam IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 261 333 4
PF09427 Pfam IPR019006 Domain of unknown function DUF2014 517 769 1
SM00353 SMART IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 266 338 4
TMhelix TMHMM 511 530 959
TMhelix TMHMM 441 463 959
PS50888 Prosite Profiles IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 260 332 4
PTHR12565 HMMPANTHER 261 397 3
PTHR12565:SF3 HMMPANTHER 261 397 2
4.10.280.10 Gene3D IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 261 351 4
SSF47459 SuperFamily IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 258 350 4
Coil ncoils Rabaptin coiled-coil domain 322 350 968

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465
SO:0100011cleaved_peptide_regionPMID:15797383295

Protein Properties

Ave. residue weight 109.43 Da
Charge 1.00
Isoelectric point 6.62
Molecular weight 98.49 kDa
Number of residues 900
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS243PMID:217125471670
present during mitotic M phase
present during mitotic M phaseexperimental evidenceS245PMID:21712547
MOD:00696phosphorylated residueIDAT249PMID:243276581922
added by hhp2
added by hhp2IDAS247PMID:24327658
added by hhp2IDAS245PMID:24327658
added by hhp2IDAT197PMID:24327658
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
926.54during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
771.83during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.5during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2.6during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:1653792378
target of proteasome PMID:19520858 PMID:184183813
autoregulation1
stability regulated by oxygen PMID:184183811
target of ERAD pathway PMID:195208581
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
membrane-tethered transcription factorInferred from Sequence or Structural Similarity PMID:117902534
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog602
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-WesternPMID:24327658
Biochemical Activity
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Affinity Capture-WesternPMID:23760507
scp1Sre1 cleavage activating protein, Scap Scp1 Affinity Capture-WesternPMID:15797383
ofd12-oxoglutarate and Fe(II) dioxygenase domain containing protein 1 Two-hybridPMID:22017871
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
pnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:21504829
ras1GTPase Ras1 Phenotypic SuppressionPMID:9745019
pvg3galactosylxylosylprotein 3-beta-galactosyltransferase Pvg3 Negative GeneticPMID:21504829
tsf1mitochondrial translation elongation factor EF-Ts Tsf1 Negative GeneticPMID:21504829
atp15F0-ATPase epsilon subunit (predicted) Positive GeneticPMID:21504829
cmk2MAPK-activated protein kinase Cmk2 Positive GeneticPMID:21504829
cdc48AAA family ATPase involved in ubiquitin-mediated protein degradation Cdc48 Dosage RescuePMID:22086920
SPCC594.01DUF1769 family protein Negative GeneticPMID:21504829
zds1zds family protein phosphatase type A regulator Zds1 (predicted) Positive GeneticPMID:21504829
aca1L-azetidine-2-carboxylic acid acetyltransferase Aca1 Negative GeneticPMID:21504829
SPBC56F2.08cRNA-binding protein (predicted) Positive GeneticPMID:21504829
lys2homoaconitate hydratase Lys2 Negative GeneticPMID:21504829
arg11N-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase Negative GeneticPMID:21504829
hri2eIF2 alpha kinase Hri2 Positive GeneticPMID:21504829
SPAC9G1.07Schizosaccharomyces specific protein Negative GeneticPMID:21504829
atg6beclin family protein, involved in autophagy Negative GeneticPMID:21504829
lys4homocitrate synthase Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
ofd12-oxoglutarate and Fe(II) dioxygenase domain containing protein 1 Phenotypic SuppressionPMID:24327658
SPBC13E7.03cRNA hairpin binding protein (predicted) Positive GeneticPMID:22681890
SPBC1271.14glutamate N-acetyltransferase (predicted) Negative GeneticPMID:21504829
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Dosage RescuePMID:22086920
gpd3glyceraldehyde 3-phosphate dehydrogenase Gpd3 Positive GeneticPMID:21504829
def1RNAPII degradation factor Def1 (predicted) Positive GeneticPMID:21504829
est1telomerase regulator Est1 Negative GeneticPMID:21504829
cut8tethering factor for nuclear proteasome Cut8 Negative GeneticPMID:21504829
SPAC977.05cconserved fungal family Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
tom70mitochondrial TOM complex subunit Tom70 (predicted) Phenotypic SuppressionPMID:24327658
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Dosage RescuePMID:22086920
lys3saccharopine dehydrogenase Lys3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
ccr1NADPH-cytochrome p450 reductase Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
arg3ornithine carbamoyltransferase Arg3 Negative GeneticPMID:21504829
spc2signal peptidase subunit Spc2 (predicted) Positive GeneticPMID:21504829
SPBC365.16conserved protein Negative GeneticPMID:21504829
dsc5UBX domain containing protein required for Sre1 cleavage Dosage RescuePMID:22086920
SPAC17H9.13cglutamate 5-kinase (predicted) Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
arg6acetylglutamate synthase Arg6 (predicted) Negative GeneticPMID:22681890
SPAC227.17cconserved protein Positive GeneticPMID:21504829
hhp2serine/threonine protein kinase Hhp2 Phenotypic SuppressionPMID:24327658
scp1Sre1 cleavage activating protein, Scap Scp1 Dosage RescuePMID:22086920
External References
Database Identifier Description
NBRP SPBC19C2.09 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC19C2.09 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC19C2.09 BioGRID Interaction Datasets
Expression Viewer SPBC19C2.09 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC19C2.09 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC19C2.09 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC19C2.09 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC19C2.09 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC19C2.09 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC19C2.09 Cell Cycle Data
GEO SPBC19C2.09 GEO profiles
PInt SPBC19C2.09 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC19C2.09 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC19C2.09 Fission yeast phenotypic data & analysis
SPD / RIKEN39/39A03Orfeome Localization Data
UniProtKB/SwissProtQ9UUD1Sterol regulatory element-binding protein 1
ModBaseQ9UUD1Database of comparative protein structure models
STRINGQ9UUD1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595694sterol regulatory element binding protein, transcription factor Sre1
RefSeq mRNANM_001021591972h- sterol regulatory element binding protein, transcription factor Sre1 (sre1), mRNA
European Nucleotide ArchiveCAB52036.1ENA Protein Mapping
UniParcUPI000006C498UniProt Archive

Literature for sre1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014