sre1 (SPBC19C2.09)


Gene Standard Namesre1 Characterisation Statuspublished
Systematic IDSPBC19C2.09 Feature Typeprotein coding
Synonyms Name Description
Productsterol regulatory element binding protein, transcription factor Sre1 Product Size900aa, 98.49 kDa
Genomic Location Chromosome II, 1691270-1694691 (3422nt); CDS:1691828-1694530 (2703nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
cellular response to hypoxia11
Annotation ExtensionEvidenceWith/FromReference
oxygen metabolic process2
Annotation ExtensionEvidenceWith/FromReference
positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of phospholipid biosynthetic process by positive regulation of transcription from RNA polymerase II promoter1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription from RNA polymerase II promoter78
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription from RNA polymerase II promoter in response to hypoxia2
regulation of transcription, start site selection2
Annotation ExtensionEvidenceWith/FromReference
SREBP signaling pathway13
Annotation ExtensionEvidenceWith/FromReference
transcription from RNA polymerase II promoter339
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
integral component of cytoplasmic side of endoplasmic reticulum membrane4
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
SREBP-SCAP complex3
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
sensitive to cobalt (G409E)57
sre1Δ
(M410E)
(L413T)
(G412E)
viable vegetative cell populationsre1Δ3837

Cell Phenotype

Term NameGenotypesCount
decreased protein processing15
affecting sre1 (G409E)
affecting sre1 (N408E)
affecting sre1 (M410E)
affecting sre1 (G412E)
affecting sre1 (L413T)
viable vegetative cell with normal cell morphologysre1Δ3100
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in dsc1 Golgi Dsc E3 ligase complex subunit Dsc1
FYPO affected by mutation in hhp2 serine/threonine protein kinase Hhp2
FYPO affected by mutation in scp1 Sre1 cleavage activating protein, Scap Scp1
FYPO affected by mutation in sre1 sterol regulatory element binding protein, transcription factor Sre1
FYPO affected by mutation in tom70 mitochondrial TOM complex subunit Tom70 (predicted)
FYPO localization affected by mutation in dsc1 Golgi Dsc E3 ligase complex subunit Dsc1
FYPO localization affected by mutation in dsc2 Golgi Dsc E3 ligase complex subunit Dsc2
FYPO localization affected by mutation in dsc3 Golgi Dsc E3 ligase complex subunit Dsc3
FYPO localization affected by mutation in dsc4 Golgi Dsc E3 ligase complex subunit Dsc4
GO regulated by dsc1 Golgi Dsc E3 ligase complex subunit Dsc1
GO regulated by dsc2 Golgi Dsc E3 ligase complex subunit Dsc2
GO regulated by dsc3 Golgi Dsc E3 ligase complex subunit Dsc3
GO regulated by dsc4 Golgi Dsc E3 ligase complex subunit Dsc4
GO regulated by nro1 negative regulator of Ofd1, Nro1
GO regulated by ofd1 2-oxoglutarate and Fe(II) dioxygenase domain containing protein 1
GO regulated by rpn12 19S proteasome regulatory subunit Rpn12
GO regulated by scp1 Sre1 cleavage activating protein, Scap Scp1
GO regulated by sre1 sterol regulatory element binding protein, transcription factor Sre1
GO regulated by ubc4 APC ubiquitin conjugating enzyme E2 Ubc4/UbcP1
GO substrate of hhp2 serine/threonine protein kinase Hhp2
GO substrate of hrd1 synviolin family ubiquitin-protein ligase E3, Hrd1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1691270..1694691
5' UTR1691270..1691827PMID:21511999
3' UTR1694531..1694691PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00010 Pfam IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 261 333 4
PF09427 Pfam IPR019006 Domain of unknown function DUF2014 518 765 1
SM00353 SMART IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 266 338 4
TMhelix TMHMM 441 463 953
TMhelix TMHMM 511 530 953
PS50888 Prosite Profiles IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 260 332 4
PTHR12565:SF124 HMMPANTHER 209 288 2
PTHR12565:SF124 HMMPANTHER 308 522 2
PTHR12565:SF124 HMMPANTHER 72 145 2
PTHR12565 HMMPANTHER 209 288 3
PTHR12565 HMMPANTHER 308 522 3
PTHR12565 HMMPANTHER 72 145 3
4.10.280.10 Gene3D IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 261 351 4
SSF47459 SuperFamily IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 258 350 4
Coil ncoils Predicted coiled-coil protein (DUF2205) 322 349 1057

View domain organization at Pfam

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465
SO:0100011cleaved_peptide_region296
PMID:15797383

Protein Properties

Ave. residue weight 109.43 Da
Charge 1.00
Codon Adaptation Index 0.48
Isoelectric point 6.62
Molecular weight 98.49 kDa
Number of residues 900
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS243
present during mitotic M phaseS245
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S243 PMID:21712547
present during mitotic M phase experimental evidence S245 PMID:21712547
phosphorylated residue 1930
added by hhp2T249
added by hhp2S245
added by hhp2S247
added by hhp2T197
Annotation ExtensionEvidenceResidueReference
added by hhp2 IDA T249 PMID:24327658
added by hhp2 IDA S245 PMID:24327658
added by hhp2 IDA S247 PMID:24327658
added by hhp2 IDA T197 PMID:24327658
ubiquitinylated lysineK558, K570 512
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence K558 PMID:26412298
mass spectrometry evidence K570 PMID:26412298
IDA PMID:26412298
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0071456expression microarray evidencePMID:22235339

Quantitative Gene Expression

View graphical display of gene expression data for sre1 (SPBC19C2.09)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
926.54during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
771.83during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.5during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2.6during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:1653792377
target of proteasome PMID:18418381 PMID:195208583
autoregulation1
stability regulated by oxygen PMID:184183811
target of ERAD pathway PMID:195208581
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
membrane-tethered transcription factorInferred from Sequence or Structural Similarity PMID:117902534
Taxonomic Conservation
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog601
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC19C2.09 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
forms complex withscp1Sre1 cleavage activating protein, Scap Scp1 Reconstituted ComplexPMID:25771684
binds DNA-binding domain construct withgmh6alpha-1,2-galactosyltransferase Gmh6 Two-hybridPMID:26771498
binds DNA-binding domain construct withubc6ubiquitin conjugating enzyme E2 Ubc6 (predicted) Two-hybridPMID:26771498
binds activation domain construct withsre1sterol regulatory element binding protein, transcription factor Sre1 Two-hybridPMID:26771498
binds activation domain construct withofd12-oxoglutarate and Fe(II) dioxygenase domain containing protein 1 Two-hybridPMID:22017871
binds activation domain construct withemc4ER membrane protein complex subunit 4 (predicted) Two-hybridPMID:26771498
binds activation domain construct withpep12SNARE Pep12 Two-hybridPMID:26771498
modified byhhp2serine/threonine protein kinase Hhp2 Biochemical ActivityPMID:24327658
affinity captured byscp1Sre1 cleavage activating protein, Scap Scp1 Affinity Capture-WesternPMID:15797383
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-WesternPMID:24327658
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC19C2.09 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withSPAC977.05cconserved fungal family Negative GeneticPMID:22681890
negative genetic interaction withatg6autophagy associated beclin family protein Atg6 Negative GeneticPMID:21504829
negative genetic interaction withccr1NADPH-cytochrome p450 reductase Negative GeneticPMID:22681890
negative genetic interaction withpnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:21504829
negative genetic interaction withest1telomerase regulator Est1 Negative GeneticPMID:21504829
negative genetic interaction witharg6acetylglutamate synthase Arg6 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withlys2homoaconitate hydratase Lys2 Negative GeneticPMID:21504829
negative genetic interaction withlys4homocitrate synthase Negative GeneticPMID:21504829
negative genetic interaction withSPAC17H9.13cglutamate 5-kinase (predicted) Negative GeneticPMID:21504829
negative genetic interaction withtsf1mitochondrial translation elongation factor EF-Ts Tsf1 Negative GeneticPMID:21504829
negative genetic interaction withlys3saccharopine dehydrogenase Lys3 Negative GeneticPMID:21504829
negative genetic interaction withpvg3galactosylxylosylprotein 3-beta-galactosyltransferase Pvg3 Negative GeneticPMID:21504829
negative genetic interaction withSPAC9G1.07Schizosaccharomyces specific protein Negative GeneticPMID:21504829
negative genetic interaction witharg3ornithine carbamoyltransferase Arg3 Negative GeneticPMID:21504829
negative genetic interaction witharg11N-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase Negative GeneticPMID:21504829
negative genetic interaction withaca1L-azetidine-2-carboxylic acid acetyltransferase Aca1 Negative GeneticPMID:21504829
negative genetic interaction withcut8tethering factor for nuclear proteasome Cut8 Negative GeneticPMID:21504829
negative genetic interaction withSPBC1271.14glutamate N-acetyltransferase (predicted) Negative GeneticPMID:21504829
negative genetic interaction withSPCC594.01DUF1769 family protein Negative GeneticPMID:21504829
negative genetic interaction withSPBC365.16conserved protein Negative GeneticPMID:21504829
overexpression rescuesdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Dosage RescuePMID:22086920
overexpression rescuesdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Dosage RescuePMID:22086920
overexpression rescuescdc48AAA family ATPase involved in ubiquitin-mediated protein degradation Cdc48 Dosage RescuePMID:22086920
overexpression rescuesdsc5UBX domain containing protein required for Sre1 cleavage Dosage RescuePMID:22086920
overexpression rescuesscp1Sre1 cleavage activating protein, Scap Scp1 Dosage RescuePMID:22086920
rescuesras1GTPase Ras1 Phenotypic SuppressionPMID:9745019
rescued byhhp2serine/threonine protein kinase Hhp2 Phenotypic SuppressionPMID:24327658
positive genetic interaction withSPAC227.17cconserved eukaryotic protein, DUF3128, human c22orf39 ortholog Positive GeneticPMID:21504829
positive genetic interaction withcmk2MAPK-activated protein kinase Cmk2 Positive GeneticPMID:21504829
positive genetic interaction withhri2eIF2 alpha kinase Hri2 Positive GeneticPMID:21504829
positive genetic interaction withSPBC13E7.03cRNA hairpin binding protein (predicted) Positive GeneticPMID:22681890
positive genetic interaction withspc2signal peptidase subunit Spc2 (predicted) Positive GeneticPMID:21504829
positive genetic interaction withgpd3glyceraldehyde 3-phosphate dehydrogenase Gpd3 Positive GeneticPMID:21504829
positive genetic interaction withpuf1pumilio family RNA-binding protein Puf1 (predicted) Positive GeneticPMID:21504829
positive genetic interaction withzds1zds family protein phosphatase type A regulator Zds1 (predicted) Positive GeneticPMID:21504829
positive genetic interaction withdef1RNAPII degradation factor Def1 (predicted) Positive GeneticPMID:21504829
positive genetic interaction withatp15F0-ATPase epsilon subunit (predicted) Positive GeneticPMID:21504829
rescued by overexpression oftom70mitochondrial TOM complex subunit Tom70 (predicted) Dosage RescuePMID:24327658
rescued by overexpression ofhhp2serine/threonine protein kinase Hhp2 Dosage RescuePMID:24327658
rescued by overexpression ofofd12-oxoglutarate and Fe(II) dioxygenase domain containing protein 1 Dosage RescuePMID:24327658
External References
Database Identifier Description
NBRP SPBC19C2.09 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC19C2.09 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC19C2.09 BioGRID Interaction Datasets
Expression Viewer SPBC19C2.09 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC19C2.09 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC19C2.09 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC19C2.09 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC19C2.09 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC19C2.09 Transcriptome Viewer (Bähler Lab)
GEO SPBC19C2.09 GEO profiles
PInt SPBC19C2.09 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC19C2.09 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC19C2.09 Fission yeast phenotypic data & analysis
Cyclebase SPBC19C2.09.1 Cell Cycle Data
SPD / RIKEN39/39A03Orfeome Localization Data
UniProtKB/SwissProtQ9UUD1Sterol regulatory element-binding protein 1 Processed sterol regulatory element-binding protein 1
ModBaseQ9UUD1Database of comparative protein structure models
STRINGQ9UUD1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595694sterol regulatory element binding protein, transcription factor Sre1
RefSeq mRNANM_001021591972h- sterol regulatory element binding protein, transcription factor Sre1 (sre1), mRNA

Literature for sre1

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016