sre1 (SPBC19C2.09)


Gene Standard Namesre1 Characterisation Statuspublished
Systematic IDSPBC19C2.09 Feature Typeprotein coding
Synonyms Name Description
Productsterol regulatory element binding protein, transcription factor Sre1 Product Size900aa, 98.49 kDa
Genomic Location Chromosome II, 1691270-1694691 (3422nt); CDS:1691828-1694530 (2703nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
cellular response to hypoxia7
Annotation ExtensionEvidenceWith/FromReference
negative regulation of transcription from RNA polymerase II promoter80
Annotation ExtensionEvidenceWith/FromReference
oxygen metabolic process2
Annotation ExtensionEvidenceWith/FromReference
positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of heme biosynthetic process1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of phospholipid biosynthetic process by positive regulation of transcription from RNA polymerase II promoter1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription from RNA polymerase II promoter87
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription from RNA polymerase II promoter in response to hypoxia2
regulation of transcription, start site selection2
Annotation ExtensionEvidenceWith/FromReference
SREBP signaling pathway7
Annotation ExtensionEvidenceWith/FromReference
transcription from RNA polymerase II promoter334
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
integral component of cytoplasmic side of endoplasmic reticulum membrane4
Annotation ExtensionEvidenceWith/FromReference
nucleus2697
Annotation ExtensionEvidenceWith/FromReference
SREBP-SCAP complex3
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
sensitive to cobaltsre1ΔNull56
unnamed (G409E)Not specified
unnamed (G412E)Not specified
unnamed (L413T)Not specified
unnamed (M410E)Not specified
viable vegetative cell populationsre1ΔNull3783
unnamed (unrecorded)

Cell Phenotype

Term NameAlleleExpressionCount
decreased protein processing13
affecting sre1unnamed (G409E)Endogenous
affecting sre1unnamed (G412E)Endogenous
affecting sre1unnamed (L413T)Endogenous
affecting sre1unnamed (M410E)Endogenous
affecting sre1unnamed (N408E)Endogenous
viable vegetative cell with normal cell morphologysre1ΔNull3092
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in hhp2 serine/threonine protein kinase Hhp2 PMID:24327658
FYPO affected by mutation in scp1 Sre1 cleavage activating protein, Scap Scp1 PMID:18503029
FYPO affected by mutation in sre1 sterol regulatory element binding protein, transcription factor Sre1 PMID:23729666
FYPO affected by mutation in tom70 mitochondrial TOM complex subunit Tom70 (predicted) PMID:24327658
GO regulated by nro1 negative regulator of Ofd1, Nro1 PMID:22017871
GO regulated by ofd1 2-oxoglutarate and Fe(II) dioxygenase domain containing protein 1 PMID:22017871
GO regulated by scp1 Sre1 cleavage activating protein, Scap Scp1 PMID:15797383
GO regulated by sre1 sterol regulatory element binding protein, transcription factor Sre1 PMID:16537923
GO substrate of hhp2 serine/threonine protein kinase Hhp2 PMID:24327658
GO substrate of hrd1 synviolin family ubiquitin-protein ligase E3, Hrd1 PMID:19520858
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
116912701694691

UTRs

Region Coordinates Reference
five_prime_UTR1691270..1691827PMID:21511999
three_prime_UTR1694531..1694691PMID:21511999
mRNA1691270..1694691
exon1691828..1694530
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00010 Pfam IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 261 333 4
PF09427 Pfam IPR019006 Domain of unknown function DUF2014 517 769 1
SM00353 SMART IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 266 338 4
TMhelix TMHMM 511 530 959
TMhelix TMHMM 441 463 959
PS50888 Prosite Profiles IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 260 332 4
PTHR12565:SF3 HMMPANTHER 31 438 2
PTHR12565 HMMPANTHER 31 438 3
4.10.280.10 Gene3D IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 261 351 4
SSF47459 SuperFamily IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 258 350 4
Coil ncoils Rabaptin coiled-coil domain 322 350 968

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465
SO:0100011cleaved_peptide_region295
PMID:15797383

Protein Properties

Ave. residue weight 109.43 Da
Charge 1.00
Isoelectric point 6.62
Molecular weight 98.49 kDa
Number of residues 900
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS245
present during mitotic M phaseS243
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S243 PMID:21712547
present during mitotic M phase experimental evidence S245 PMID:21712547
phosphorylated residue 1923
added by hhp2T197
added by hhp2S245
added by hhp2S247
added by hhp2T249
Annotation ExtensionEvidenceResidueReference
added by hhp2 IDA S245 PMID:24327658
added by hhp2 IDA S247 PMID:24327658
added by hhp2 IDA T197 PMID:24327658
added by hhp2 IDA T249 PMID:24327658
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
926.54during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
771.83during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.5during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2.6during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:1653792378
target of proteasome PMID:19520858 PMID:184183813
autoregulation1
stability regulated by oxygen PMID:184183811
target of ERAD pathway PMID:195208581
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
membrane-tethered transcription factorInferred from Sequence or Structural Similarity PMID:117902534
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog597
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bydsc2Golgi Dsc E3 ligase complex subunit Dsc2 Affinity Capture-WesternPMID:23760507
modified byhhp2serine/threonine protein kinase Hhp2 Biochemical ActivityPMID:24327658
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-WesternPMID:24327658
binds activation domain construct withofd12-oxoglutarate and Fe(II) dioxygenase domain containing protein 1 Two-hybridPMID:22017871
affinity captured byscp1Sre1 cleavage activating protein, Scap Scp1 Affinity Capture-WesternPMID:15797383
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withaca1L-azetidine-2-carboxylic acid acetyltransferase Aca1 Negative GeneticPMID:21504829
negative genetic interaction witharg11N-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase Negative GeneticPMID:21504829
negative genetic interaction witharg3ornithine carbamoyltransferase Arg3 Negative GeneticPMID:21504829
negative genetic interaction witharg6acetylglutamate synthase Arg6 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withatg6beclin family protein, involved in autophagy Negative GeneticPMID:21504829
positive genetic interaction withatp15F0-ATPase epsilon subunit (predicted) Positive GeneticPMID:21504829
negative genetic interaction withccr1NADPH-cytochrome p450 reductase Negative GeneticPMID:21504829
negative genetic interaction withccr1NADPH-cytochrome p450 reductase Negative GeneticPMID:22681890
overexpression rescuescdc48AAA family ATPase involved in ubiquitin-mediated protein degradation Cdc48 Dosage RescuePMID:22086920
positive genetic interaction withcmk2MAPK-activated protein kinase Cmk2 Positive GeneticPMID:21504829
negative genetic interaction withcut8tethering factor for nuclear proteasome Cut8 Negative GeneticPMID:21504829
positive genetic interaction withdef1RNAPII degradation factor Def1 (predicted) Positive GeneticPMID:21504829
overexpression rescuesdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Dosage RescuePMID:22086920
overexpression rescuesdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Dosage RescuePMID:22086920
overexpression rescuesdsc5UBX domain containing protein required for Sre1 cleavage Dosage RescuePMID:22086920
negative genetic interaction withest1telomerase regulator Est1 Negative GeneticPMID:21504829
positive genetic interaction withgpd3glyceraldehyde 3-phosphate dehydrogenase Gpd3 Positive GeneticPMID:21504829
rescued byhhp2serine/threonine protein kinase Hhp2 Phenotypic SuppressionPMID:24327658
positive genetic interaction withhri2eIF2 alpha kinase Hri2 Positive GeneticPMID:21504829
negative genetic interaction withlys2homoaconitate hydratase Lys2 Negative GeneticPMID:21504829
negative genetic interaction withlys3saccharopine dehydrogenase Lys3 Negative GeneticPMID:21504829
negative genetic interaction withlys3saccharopine dehydrogenase Lys3 Negative GeneticPMID:22681890
negative genetic interaction withlys4homocitrate synthase Negative GeneticPMID:21504829
negative genetic interaction withlys4homocitrate synthase Negative GeneticPMID:22681890
rescued byofd12-oxoglutarate and Fe(II) dioxygenase domain containing protein 1 Phenotypic SuppressionPMID:24327658
negative genetic interaction withpnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:21504829
negative genetic interaction withpvg3galactosylxylosylprotein 3-beta-galactosyltransferase Pvg3 Negative GeneticPMID:21504829
rescuesras1GTPase Ras1 Phenotypic SuppressionPMID:9745019
overexpression rescuesscp1Sre1 cleavage activating protein, Scap Scp1 Dosage RescuePMID:22086920
negative genetic interaction withSPAC17H9.13cglutamate 5-kinase (predicted) Negative GeneticPMID:21504829
negative genetic interaction withSPAC17H9.13cglutamate 5-kinase (predicted) Negative GeneticPMID:22681890
positive genetic interaction withSPAC227.17cconserved protein Positive GeneticPMID:21504829
negative genetic interaction withSPAC977.05cconserved fungal family Negative GeneticPMID:21504829
negative genetic interaction withSPAC977.05cconserved fungal family Negative GeneticPMID:22681890
negative genetic interaction withSPAC9G1.07Schizosaccharomyces specific protein Negative GeneticPMID:21504829
negative genetic interaction withSPBC1271.14glutamate N-acetyltransferase (predicted) Negative GeneticPMID:21504829
positive genetic interaction withSPBC13E7.03cRNA hairpin binding protein (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPBC365.16conserved protein Negative GeneticPMID:21504829
positive genetic interaction withSPBC56F2.08cRNA-binding protein (predicted) Positive GeneticPMID:21504829
positive genetic interaction withspc2signal peptidase subunit Spc2 (predicted) Positive GeneticPMID:21504829
negative genetic interaction withSPCC594.01DUF1769 family protein Negative GeneticPMID:21504829
rescued bytom70mitochondrial TOM complex subunit Tom70 (predicted) Phenotypic SuppressionPMID:24327658
negative genetic interaction withtsf1mitochondrial translation elongation factor EF-Ts Tsf1 Negative GeneticPMID:21504829
positive genetic interaction withzds1zds family protein phosphatase type A regulator Zds1 (predicted) Positive GeneticPMID:21504829
External References
Database Identifier Description
NBRP SPBC19C2.09 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC19C2.09 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC19C2.09 BioGRID Interaction Datasets
Expression Viewer SPBC19C2.09 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC19C2.09 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC19C2.09 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC19C2.09 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC19C2.09 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC19C2.09 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC19C2.09 Cell Cycle Data
GEO SPBC19C2.09 GEO profiles
PInt SPBC19C2.09 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC19C2.09 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC19C2.09 Fission yeast phenotypic data & analysis
SPD / RIKEN39/39A03Orfeome Localization Data
UniProtKB/SwissProtQ9UUD1Sterol regulatory element-binding protein 1
ModBaseQ9UUD1Database of comparative protein structure models
STRINGQ9UUD1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595694sterol regulatory element binding protein, transcription factor Sre1
RefSeq mRNANM_001021591972h- sterol regulatory element binding protein, transcription factor Sre1 (sre1), mRNA
European Nucleotide ArchiveCAB52036.1ENA Protein Mapping
UniParcUPI000006C498UniProt Archive

Literature for sre1

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015