sre1 (SPBC19C2.09)


Gene Standard Namesre1 Characterisation Statuspublished
Systematic IDSPBC19C2.09 Feature Typeprotein coding
Synonyms Name Description
Productsterol regulatory element binding protein, transcription factor Sre1 Product Size900aa, 98.49 kDa
Genomic Location Chromosome II, 1691270-1694691 (3422nt); CDS:1691828-1694530 (2703nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003677DNA bindingIDAPMID:16537923385
GO:0000978RNA polymerase II core promoter proximal region sequence-specific DNA bindingIMPPMID:1653792396
at sterol_regulatory_elementIDAPMID:22017871
at sterol_regulatory_elementIDAPMID:16537923
GO:0001078RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription8
during cellular response to hypoxia, regulates SPCC4G3.17IMPPMID:16537923
during cellular response to hypoxia, regulates SPAC3A11.07IMPPMID:16537923
GO:0001077RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription26
has direct input hem13IMPPMID:22017871
GO:0005515protein bindingIPIhrd1PMID:19520858887
GO:0046983protein dimerization activityIEAIPR011598GO_REF:0000002109
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032933SREBP signaling pathwayIMPPMID:157973834
GO:0071456cellular response to hypoxiaIMPPMID:220178718
IMPPMID:15797383
GO:0000122negative regulation of transcription from RNA polymerase II promoterIMPPMID:1653792374
GO:0072592oxygen metabolic process2
requiredIMPPMID:15797383
GO:0035961positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoterIMPPMID:157973831
GO:0070455positive regulation of heme biosynthetic processIEPPMID:165379231
GO:1900413positive regulation of phospholipid biosynthetic process by positive regulation of transcription from RNA polymerase II promoterIMPPMID:165379231
GO:0045944positive regulation of transcription from RNA polymerase II promoter85
regulates hem13IMPPMID:15797383
regulates erg25IMPPMID:15797383
regulates erg31IMPPMID:15797383
GO:0061419positive regulation of transcription from RNA polymerase II promoter in response to hypoxia2
during cellular response to hypoxia, regulates erg11IMPPMID:16537923
during cellular response to hypoxia, regulates hem15IMPPMID:16537923
during cellular response to hypoxia, regulates scs7IMPPMID:16537923
during cellular response to hypoxia, regulates dap1IMPPMID:16537923
during cellular response to hypoxia, regulates SPBC106.12cIMPPMID:16537923
during cellular response to hypoxia, regulates erg25IMPPMID:16537923
during cellular response to hypoxia, regulates css1IMPPMID:16537923
during cellular response to hypoxia, regulates osm1IMPPMID:16537923
during cellular response to hypoxia, regulates mmd1IMPPMID:16537923
during cellular response to hypoxia, regulates sur2IMPPMID:16537923
during cellular response to hypoxia, regulates SPBC215.11cIMPPMID:16537923
during cellular response to hypoxia, regulates erg31IMPPMID:16537923
during cellular response to hypoxia, regulates hem14IMPPMID:16537923
during cellular response to hypoxia, regulates hem13IMPPMID:16537923
during cellular response to hypoxia, regulates sre1IMPPMID:16537923
GO:0010630regulation of transcription, start site selectionIEPPMID:182766452
GO:0006366transcription from RNA polymerase II promoterIMPPMID:16537923130
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032936SREBP-SCAP complexIDAPMID:157973833
GO:0071458integral component of cytoplasmic side of endoplasmic reticulum membraneTASPMID:165379234
GO:0005634nucleusIEAUniProtKB-KW:KW-0539GO_REF:00000372730
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001245sensitive to cobaltCell growth assaynot recorded (M410E)Not specifiedPMID:2372966656
Cell growth assaynot recorded (G409E)Not specifiedPMID:23729666
Cell growth assaynot recorded (L413T)Not specifiedPMID:23729666
Cell growth assaynot recorded (G412E)Not specifiedPMID:23729666
Cell growth assaysre1ΔNullPMID:23729666
FYPO:0002060viable vegetative cell populationMicroscopysre1ΔNullPECO:0000137, PECO:0000005PMID:236978063755
no_namePMID:15797383
Microscopysre1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001422decreased protein processingWestern blot assaynot recorded (G409E)EndogenousPMID:2372966611
affecting sre1
affecting sre1Western blot assaynot recorded (G412E)EndogenousPMID:23729666
affecting sre1Western blot assaynot recorded (M410E)EndogenousPMID:23729666
affecting sre1Western blot assaynot recorded (N408E)EndogenousPMID:23729666
affecting sre1Western blot assaynot recorded (L413T)EndogenousPMID:23729666
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopysre1ΔNullPECO:0000137, PECO:0000005PMID:236978063078
penetrance FYPO_EXT:0000001
Target Of
RelationshipGeneProductReference
substrate of hrd1 ubiquitin-protein ligase Hrd1, synviolin family PMID:19520858
regulated by nro1 negative regulator of Ofd1, Nro1 PMID:22017871
regulated by ofd1 2-oxoglutarate and Fe(II) dioxygenase domain containing protein 1 PMID:22017871
regulated by scp1 Sre1 cleavage activating protein, Scap Scp1 PMID:15797383
regulated by sre1 sterol regulatory element binding protein, transcription factor Sre1 PMID:16537923
substrate of ubc7 ubiquitin conjugating enzyme Ubc7/UbcP3 PMID:19520858
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
116912701694691

UTRs

Region Start End Reference
five_prime_UTR16912701691827PMID:21511999
three_prime_UTR16945311694691PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00010 Pfam IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 261 333 4
PF09427 Pfam IPR019006 Domain of unknown function DUF2014 517 769 1
SM00353 SMART IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 266 338 4
TMhelix TMHMM 441 463 959
TMhelix TMHMM 511 530 959
PS50888 Prosite Profiles IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 260 332 4
PTHR12565 HMMPANTHER 261 397 3
PTHR12565:SF3 HMMPANTHER 261 397 2
G3DSA:4.10.280.10 Gene3D IPR011598 261 351 4
SSF47459 SuperFamily IPR011598 258 350 4
Coil ncoils Rabaptin coiled-coil domain 322 350 975
Low complexity (SEG) seg 721 730
Low complexity (SEG) seg 3 16
Low complexity (SEG) seg 35 53
Low complexity (SEG) seg 85 96
Low complexity (SEG) seg 368 385
Low complexity (SEG) seg 680 694

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465
SO:0100011cleaved_peptide_regionPMID:15797383292

Protein Properties

Ave. residue weight 109.43 Da
Charge 1.00
Isoelectric point 6.62
Molecular weight 98.49 kDa
Number of residues 900
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
771.83during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population_wideexperimental evidencePMID:23101633
926.54during GO:0072690PECO:0000014,
PECO:0000005
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.6during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population_wideexperimental evidencePMID:23101633
5.5during GO:0072690PECO:0000014,
PECO:0000005
population_wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:1653792379
target of proteasome PMID:19520858 PMID:184183813
autoregulation1
stability regulated by oxygen PMID:184183811
target of ERAD pathway PMID:195208581
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
membrane-tethered transcription factorInferred from Sequence or Structural Similarity PMID:117902534
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog579
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
pvg3galactosylxylosylprotein 3-beta-galactosyltransferase Pvg1 Negative GeneticPMID:21504829
hhp2serine/threonine protein kinase Hhp2 Phenotypic SuppressionPMID:24327658
SPAC9G1.07sequence orphan Negative GeneticPMID:21504829
est1telomerase regulator Est1 Negative GeneticPMID:21504829
tom70mitochondrial TOM complex subunit Tom70 (predicted) Phenotypic SuppressionPMID:24327658
SPBC1271.14glutamate N-acetyltransferase (predicted) Negative GeneticPMID:21504829
dsc5UBX domain containing protein required for Sre1 cleavage Dosage RescuePMID:22086920
lys2homoaconitate hydratase Lys2 Negative GeneticPMID:21504829
atp15F0-ATPase epsilon subunit (predicted) Positive GeneticPMID:21504829
spc2signal peptidase subunit Spc2 (predicted) Positive GeneticPMID:21504829
hri2eIF2 alpha kinase Hri2 Positive GeneticPMID:21504829
SPAC227.17cconserved protein Positive GeneticPMID:21504829
SPAC17H9.13cglutamate 5-kinase (predicted) Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
cut8tethering factor for nuclear proteasome Cut8 Negative GeneticPMID:21504829
SPAC977.05cconserved fungal family Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
SPBC13E7.03cRNA hairpin binding protein (predicted) Positive GeneticPMID:22681890
arg6acetylglutamate synthase Arg6 (predicted) Negative GeneticPMID:22681890
SPBC365.16conserved protein Negative GeneticPMID:21504829
atg6beclin family protein, involved in autophagy Negative GeneticPMID:21504829
pnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:21504829
def1RNAPII degradation factor Def1 (predicted) Positive GeneticPMID:21504829
ras1GTPase Ras1 Phenotypic SuppressionPMID:9745019
arg11N-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase Negative GeneticPMID:21504829
aca1L-azetidine-2-carboxylic acid acetyltransferase Aca1 Negative GeneticPMID:21504829
gpd3glyceraldehyde 3-phosphate dehydrogenase Gpd3 Positive GeneticPMID:21504829
lys4homocitrate synthase Negative GeneticPMID:22681890
Negative GeneticPMID:21504829
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Dosage RescuePMID:22086920
ofd12-oxoglutarate and Fe(II) dioxygenase domain containing protein 1 Phenotypic SuppressionPMID:24327658
cdc48AAA family ATPase involved in ubiquitin-mediated protein degradation Cdc48 Dosage RescuePMID:22086920
zds1zds family protein phosphatase type A regulator Zds1 (predicted) Positive GeneticPMID:21504829
tsf1mitochondrial translation elongation factor EF-Ts Tsf1 Negative GeneticPMID:21504829
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Dosage RescuePMID:22086920
SPCC594.01DUF1769 family protein Negative GeneticPMID:21504829
arg3ornithine carbamoyltransferase Arg3 Negative GeneticPMID:21504829
ccr1NADPH-cytochrome p450 reductase Negative GeneticPMID:22681890
Negative GeneticPMID:21504829
lys3saccharopine dehydrogenase Lys3 Negative GeneticPMID:22681890
Negative GeneticPMID:21504829
SPBC56F2.08cRNA-binding protein (predicted) Positive GeneticPMID:21504829
scp1Sre1 cleavage activating protein, Scap Scp1 Dosage RescuePMID:22086920
cmk2MAPK-activated protein kinase Cmk2 Positive GeneticPMID:21504829
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Affinity Capture-WesternPMID:23760507
scp1Sre1 cleavage activating protein, Scap Scp1 Affinity Capture-WesternPMID:15797383
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-WesternPMID:24327658
Biochemical Activity
ofd12-oxoglutarate and Fe(II) dioxygenase domain containing protein 1 Two-hybridPMID:22017871
External References
Database Identifier Description
NBRP SPBC19C2.09 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC19C2.09 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC19C2.09 BioGRID Interaction Datasets
Expression Viewer SPBC19C2.09 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC19C2.09 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC19C2.09 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC19C2.09 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC19C2.09 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC19C2.09 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC19C2.09 Cell Cycle Data
GEO SPBC19C2.09 GEO profiles
PInt SPBC19C2.09 Protein-Protein Interaction Predictor (Bähler Lab)
EntrezGene2540730sterol regulatory element binding protein, transcription factor Sre1
WikiGene2540730sterol regulatory element binding protein, transcription factor Sre1
SPD / RIKEN39/39A03Orfeome Localization Data
UniProtKB/SwissProtQ9UUD1Sterol regulatory element-binding protein 1 Processed sterol regulatory element-binding protein 1
ModBaseQ9UUD1Database of comparative protein structure models
StringQ9UUD1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595694sterol regulatory element binding protein, transcription factor Sre1
RefSeq mRNANM_001021591972h- sterol regulatory element binding protein, transcription factor Sre1 (sre1), mRNA
European Nucleotide ArchiveCAB52036ENA Protein Mapping
UniParcUPI000006C498UniProt Archive

Literature for sre1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014