sre1 (SPBC19C2.09)


Gene Standard Namesre1 Characterisation Statuspublished
Systematic IDSPBC19C2.09 Feature Typeprotein coding
Synonyms Name Description
Productsterol regulatory element binding protein, transcription factor Sre1 Product Size900aa, 98.49 kDa
Genomic Location Chromosome II, 1691270-1694691 (3422nt); CDS:1691828-1694530 (2703nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
cellular response to hypoxia7
Annotation ExtensionEvidenceWith/FromReference
oxygen metabolic process2
Annotation ExtensionEvidenceWith/FromReference
positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of phospholipid biosynthetic process by positive regulation of transcription from RNA polymerase II promoter1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription from RNA polymerase II promoter73
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription from RNA polymerase II promoter in response to hypoxia2
regulation of transcription, start site selection2
Annotation ExtensionEvidenceWith/FromReference
SREBP signaling pathway7
Annotation ExtensionEvidenceWith/FromReference
transcription from RNA polymerase II promoter336
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
integral component of cytoplasmic side of endoplasmic reticulum membrane4
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
SREBP-SCAP complex3
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
sensitive to cobaltunnamed (M410E)57
unnamed (G409E)
unnamed (G412E)
sre1Δ
unnamed (L413T)
viable vegetative cell populationsre1Δ3831

Cell Phenotype

Term NameGenotypesCount
decreased protein processing15
affecting sre1unnamed (G409E)
affecting sre1unnamed (L413T)
affecting sre1unnamed (N408E)
affecting sre1unnamed (M410E)
affecting sre1unnamed (G412E)
viable vegetative cell with normal cell morphologysre1Δ3099
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in dsc1 Golgi Dsc E3 ligase complex subunit Dsc1
FYPO affected by mutation in hhp2 serine/threonine protein kinase Hhp2
FYPO affected by mutation in scp1 Sre1 cleavage activating protein, Scap Scp1
FYPO affected by mutation in sre1 sterol regulatory element binding protein, transcription factor Sre1
FYPO affected by mutation in tom70 mitochondrial TOM complex subunit Tom70 (predicted)
GO regulated by nro1 negative regulator of Ofd1, Nro1
GO regulated by ofd1 2-oxoglutarate and Fe(II) dioxygenase domain containing protein 1
GO regulated by scp1 Sre1 cleavage activating protein, Scap Scp1
GO regulated by sre1 sterol regulatory element binding protein, transcription factor Sre1
GO substrate of hhp2 serine/threonine protein kinase Hhp2
GO substrate of hrd1 synviolin family ubiquitin-protein ligase E3, Hrd1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1691270..1694691
5' UTR1691270..1691827PMID:21511999
3' UTR1694531..1694691PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF09427 Pfam IPR019006 Domain of unknown function DUF2014 518 765 1
PF00010 Pfam IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 261 333 4
SM00353 SMART IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 266 338 4
TMhelix TMHMM 441 463 960
TMhelix TMHMM 511 530 960
PS50888 Prosite Profiles IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 260 332 4
PTHR12565 HMMPANTHER 308 522 3
PTHR12565 HMMPANTHER 209 288 3
PTHR12565 HMMPANTHER 72 145 3
PTHR12565:SF124 HMMPANTHER 308 522 2
PTHR12565:SF124 HMMPANTHER 209 288 2
PTHR12565:SF124 HMMPANTHER 72 145 2
4.10.280.10 Gene3D IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 261 351 4
SSF47459 SuperFamily IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain 258 350 4
Coil ncoils Predicted coiled-coil protein (DUF2205) 322 349 1050

View domain organization at Pfam

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465
SO:0100011cleaved_peptide_region296
PMID:15797383

Protein Properties

Ave. residue weight 109.43 Da
Charge 1.00
Codon Adaptation Index 0.48
Isoelectric point 6.62
Molecular weight 98.49 kDa
Number of residues 900
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1675
present during mitotic M phaseS245
present during mitotic M phaseS243
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S243 PMID:21712547
present during mitotic M phase experimental evidence S245 PMID:21712547
phosphorylated residue 1930
added by hhp2S245
added by hhp2S247
added by hhp2T197
added by hhp2T249
Annotation ExtensionEvidenceResidueReference
added by hhp2 IDA S245 PMID:24327658
added by hhp2 IDA S247 PMID:24327658
added by hhp2 IDA T197 PMID:24327658
added by hhp2 IDA T249 PMID:24327658
ubiquitinylated lysineK558, K570 512
Annotation ExtensionEvidenceResidueReference
IDA PMID:26412298
mass spectrometry evidence K558 PMID:26412298
mass spectrometry evidence K570 PMID:26412298
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0071456expression microarray evidencePMID:22235339

Quantitative Gene Expression

View graphical display of gene expression data for sre1 (SPBC19C2.09)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
926.54during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
771.83during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.5during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2.6during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:1653792377
target of proteasome PMID:18418381 PMID:195208583
autoregulation1
stability regulated by oxygen PMID:184183811
target of ERAD pathway PMID:195208581
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
membrane-tethered transcription factorInferred from Sequence or Structural Similarity PMID:117902534
Taxonomic Conservation
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog599
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3422
conserved in vertebrates3397
conserved in eukaryotes only2504
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load gene that interact genetically with SPBC19C2.09 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withgcn20AAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC19C2.09 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC19C2.09 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC19C2.09 BioGRID Interaction Datasets
Expression Viewer SPBC19C2.09 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC19C2.09 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC19C2.09 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC19C2.09 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC19C2.09 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC19C2.09 Transcriptome Viewer (Bähler Lab)
GEO SPBC19C2.09 GEO profiles
PInt SPBC19C2.09 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC19C2.09 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC19C2.09 Fission yeast phenotypic data & analysis
Cyclebase SPBC19C2.09.1 Cell Cycle Data
SPD / RIKEN39/39A03Orfeome Localization Data
UniProtKB/SwissProtQ9UUD1Sterol regulatory element-binding protein 1 Processed sterol regulatory element-binding protein 1
ModBaseQ9UUD1Database of comparative protein structure models
STRINGQ9UUD1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595694sterol regulatory element binding protein, transcription factor Sre1
RefSeq mRNANM_001021591972h- sterol regulatory element binding protein, transcription factor Sre1 (sre1), mRNA
European Nucleotide ArchiveCU329671ENA EMBL mapping
European Nucleotide ArchiveCAB52036ENA Protein Mapping
UniParcUPI000006C498UniProt Archive

Literature for sre1

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016